EA201690274A1 - Способ сайт-специфического ферментативного мечения нуклеиновых кислот in vitro введением не встречающихся в природе нуклеотидов - Google Patents

Способ сайт-специфического ферментативного мечения нуклеиновых кислот in vitro введением не встречающихся в природе нуклеотидов

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EA201690274A1
EA201690274A1 EA201690274A EA201690274A EA201690274A1 EA 201690274 A1 EA201690274 A1 EA 201690274A1 EA 201690274 A EA201690274 A EA 201690274A EA 201690274 A EA201690274 A EA 201690274A EA 201690274 A1 EA201690274 A1 EA 201690274A1
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naturally occurring
site
swiping
nucleotides
meeting
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EA201690274A
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EA038576B1 (ru
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Флойд Е. Роумсберг
Денис А. Малышев
Линцзюнь Ли
Томас Лаверн
Чжэнтао Ли
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Дзе Скриппс Рисёч Инститьют
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Publication of EA038576B1 publication Critical patent/EA038576B1/ru

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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction

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Abstract

Настоящее изобретение относится к аналогам не встречающихся в природе нуклеотидов, содержащим преимущественно гидрофобные аналоги нуклеотидных оснований, которые образуют не встречающиеся в природе пары оснований во время опосредованной ДНК-полимеразой репликации ДНК или опосредованной РНК-полимеразой транскрипции РНК. Таким образом не встречающиеся в природе нуклеотидные основания могут быть введены сайт-специфически в олигонуклеотиды (одно- или двухцепочечные ДНК или РНК), где они могут обеспечивать сайт-специфическое расщепление или могут предоставлять реакционноспособный линкер, который может подвергаться функционализации с карго-несущим реагентом посредством реакции с первичной аминогруппой или с помощью клик-химии с алкиновой группой линкера не встречающегося в природе нуклеотидного основания.
EA201690274A 2013-08-08 2014-08-08 Способ сайт-специфического ферментативного мечения нуклеиновых кислот in vitro введением не встречающихся в природе нуклеотидов EA038576B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361863649P 2013-08-08 2013-08-08
PCT/US2014/050423 WO2015021432A1 (en) 2013-08-08 2014-08-08 A method for the site-specific enzymatic labelling of nucleic acids in vitro by incorporation of unnatural nucleotides

Publications (2)

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EA201690274A1 true EA201690274A1 (ru) 2016-07-29
EA038576B1 EA038576B1 (ru) 2021-09-16

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EA201690274A EA038576B1 (ru) 2013-08-08 2014-08-08 Способ сайт-специфического ферментативного мечения нуклеиновых кислот in vitro введением не встречающихся в природе нуклеотидов
EA202191796A EA202191796A2 (ru) 2013-08-08 2014-08-08 Способ сайт-специфического ферментативного мечения нуклеиновых кислот in vitro введением не встречающихся в природе нуклеотидов

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US (4) US10626138B2 (ru)
EP (3) EP4079745A1 (ru)
JP (4) JP2016531122A (ru)
AU (4) AU2014306271A1 (ru)
CA (1) CA2920527A1 (ru)
DK (1) DK3041854T3 (ru)
EA (2) EA038576B1 (ru)
ES (1) ES2773547T3 (ru)
HR (1) HRP20200250T1 (ru)
HU (1) HUE048710T2 (ru)
LT (1) LT3041854T (ru)
PL (1) PL3041854T3 (ru)
PT (1) PT3041854T (ru)
RS (1) RS59991B1 (ru)
SI (1) SI3041854T1 (ru)
WO (1) WO2015021432A1 (ru)

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