SG11201908579PA - Method for producing complex of rna molecule and peptide, and utilization thereof - Google Patents

Method for producing complex of rna molecule and peptide, and utilization thereof

Info

Publication number
SG11201908579PA
SG11201908579PA SG11201908579PA SG11201908579PA SG 11201908579P A SG11201908579P A SG 11201908579PA SG 11201908579P A SG11201908579P A SG 11201908579PA SG 11201908579P A SG11201908579P A SG 11201908579PA
Authority
SG
Singapore
Prior art keywords
peptide
utilization
rna molecule
producing complex
present
Prior art date
Application number
Inventor
Hiroki Ueda
Yoshihiro Shimizu
Shoko Harada
Katsuhiko Matsumoto
Original Assignee
Cubicstars Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cubicstars Inc filed Critical Cubicstars Inc
Publication of SG11201908579PA publication Critical patent/SG11201908579PA/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1062—Isolating an individual clone by screening libraries mRNA-Display, e.g. polypeptide and encoding template are connected covalently
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/322—2'-R Modification

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention provides a method for efficiently obtaining a complex in which a gene (mRNA) and a peptide as a translation product thereof are linked via a peptide receptor molecule, and utilization thereof. In the method of 5 the present invention, at least one 2'-modified nucleoside derivative is introduced into a 5' end side of an antisense strand of a template DNA. [No suitable figure] 10
SG11201908579P 2017-03-17 2018-03-15 Method for producing complex of rna molecule and peptide, and utilization thereof SG11201908579PA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2017053621 2017-03-17
PCT/JP2018/010223 WO2018168999A1 (en) 2017-03-17 2018-03-15 Method for producing complex of rna molecule and peptide, and utilization thereof

Publications (1)

Publication Number Publication Date
SG11201908579PA true SG11201908579PA (en) 2019-10-30

Family

ID=63523803

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201908579P SG11201908579PA (en) 2017-03-17 2018-03-15 Method for producing complex of rna molecule and peptide, and utilization thereof

Country Status (8)

Country Link
US (1) US11286481B2 (en)
EP (1) EP3597746B1 (en)
JP (1) JP6852917B2 (en)
CN (1) CN110637086B (en)
AU (1) AU2018236568B2 (en)
CA (1) CA3056756C (en)
SG (1) SG11201908579PA (en)
WO (1) WO2018168999A1 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7416715B2 (en) 2018-11-07 2024-01-17 公益財団法人川崎市産業振興財団 Peptide-nucleic acid complex
CN111041025B (en) 2019-12-17 2021-06-18 深圳市瑞吉生物科技有限公司 mRNA targeting molecule based on combination of N-acetylgalactosamine polypeptide and preparation method thereof
CN112111524B (en) * 2020-01-10 2024-02-27 深圳瑞吉生物科技有限公司 Preparation method of mRNA-GalNAc targeting molecule, in-vivo delivery system and application thereof
CN111235198B (en) * 2020-01-20 2021-06-15 深圳市瑞吉生物科技有限公司 Method for large-scale synthesis of long-chain RNA and site-directed modification method thereof
CN111117978B (en) * 2020-01-27 2022-07-15 黄种山 Single-chain DNA peptide ligase sDPlaseI and use method thereof
CN111088235B (en) * 2020-01-27 2022-07-12 黄种山 Double-stranded DNA peptide ligase dDPlaseI and use method thereof
CN111088236B (en) * 2020-01-27 2022-07-22 黄种山 Single-stranded RNA peptide ligase sRPoseI and use method thereof
CN111088234B (en) * 2020-01-27 2022-07-15 黄种山 Double-stranded DNA peptide ligase dDPlaseII and use method thereof
CN111744019B (en) 2020-07-01 2023-08-04 深圳瑞吉生物科技有限公司 Mannose-based mRNA targeted delivery system and application thereof
CN115010794A (en) * 2022-06-30 2022-09-06 武汉瑞佶生物科技有限公司 Protein-mediated mRNA (messenger ribonucleic acid) targeting molecule as well as preparation method and application thereof

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6261804B1 (en) 1997-01-21 2001-07-17 The General Hospital Corporation Selection of proteins using RNA-protein fusions
EP0971946B1 (en) 1997-01-21 2006-07-05 The General Hospital Corporation Selection of proteins using rna-protein fusions
US8207093B2 (en) 1997-01-21 2012-06-26 The General Hospital Corporation Selection of proteins using RNA-protein fusions
AU2003225085A1 (en) * 2002-04-19 2003-11-03 California Institute Of Technology Unnatural amino acid containing display libraries
US8715984B2 (en) * 2008-02-07 2014-05-06 Riken Modified tRNA containing unnatural base and use thereof
EP2492344B1 (en) 2009-10-22 2016-04-06 PeptiDream Inc. Rapid display method in translational synthesis of peptide
JP5818237B2 (en) * 2010-09-09 2015-11-18 国立大学法人 東京大学 Translational construction and search for active species of special peptide compound library containing N-methylamino acid and other special amino acids
JP5920835B2 (en) * 2010-12-01 2016-05-18 田辺三菱製薬株式会社 Polynucleotide construct capable of presenting Fab in cell-free translation system, and Fab production method and screening method using the same
WO2012074129A1 (en) * 2010-12-03 2012-06-07 国立大学法人東京大学 Peptide with safer secondary structure, peptide library, and production methods for same
EP2657334B1 (en) * 2012-04-26 2016-07-06 GeneFrontier Corporation Efficient method for displaying protein multimer
WO2014119600A1 (en) * 2013-01-30 2014-08-07 ペプチドリーム株式会社 Flexible display method
CN103382579B (en) * 2013-07-06 2015-10-21 中国科学院成都生物研究所 A kind of method of in-vitro screening polypeptide
JP6325632B2 (en) 2016-11-02 2018-05-16 東芝ライフスタイル株式会社 refrigerator

Also Published As

Publication number Publication date
US20200283761A1 (en) 2020-09-10
CA3056756A1 (en) 2018-09-20
CA3056756C (en) 2021-12-07
AU2018236568A1 (en) 2019-11-07
WO2018168999A1 (en) 2018-09-20
EP3597746A4 (en) 2020-12-23
AU2018236568B2 (en) 2021-07-01
EP3597746B1 (en) 2023-04-19
JP6852917B2 (en) 2021-03-31
US11286481B2 (en) 2022-03-29
EP3597746A1 (en) 2020-01-22
CN110637086B (en) 2024-02-06
CN110637086A (en) 2019-12-31
JPWO2018168999A1 (en) 2020-02-27

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