RS59991B1 - Metoda enzimskog in vitro obeležavanja specifičnog za položaj nukleinskih kiselina uvođenjem neprirodnih nukleotida - Google Patents

Metoda enzimskog in vitro obeležavanja specifičnog za položaj nukleinskih kiselina uvođenjem neprirodnih nukleotida

Info

Publication number
RS59991B1
RS59991B1 RS20200210A RSP20200210A RS59991B1 RS 59991 B1 RS59991 B1 RS 59991B1 RS 20200210 A RS20200210 A RS 20200210A RS P20200210 A RSP20200210 A RS P20200210A RS 59991 B1 RS59991 B1 RS 59991B1
Authority
RS
Serbia
Prior art keywords
incorporation
vitro
site
nucleic acids
specific enzymatic
Prior art date
Application number
RS20200210A
Other languages
English (en)
Inventor
Floyd E Romesberg
Denis A Malyshev
Lingjun Li
Thomas Lavergne
Zhengtao Li
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Publication of RS59991B1 publication Critical patent/RS59991B1/sr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/24—Heterocyclic radicals containing oxygen or sulfur as ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
RS20200210A 2013-08-08 2014-08-08 Metoda enzimskog in vitro obeležavanja specifičnog za položaj nukleinskih kiselina uvođenjem neprirodnih nukleotida RS59991B1 (sr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361863649P 2013-08-08 2013-08-08
PCT/US2014/050423 WO2015021432A1 (en) 2013-08-08 2014-08-08 A method for the site-specific enzymatic labelling of nucleic acids in vitro by incorporation of unnatural nucleotides
EP14834827.9A EP3041854B1 (en) 2013-08-08 2014-08-08 A method for the site-specific enzymatic labelling of nucleic acids in vitro by incorporation of unnatural nucleotides

Publications (1)

Publication Number Publication Date
RS59991B1 true RS59991B1 (sr) 2020-04-30

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
RS20200210A RS59991B1 (sr) 2013-08-08 2014-08-08 Metoda enzimskog in vitro obeležavanja specifičnog za položaj nukleinskih kiselina uvođenjem neprirodnih nukleotida

Country Status (16)

Country Link
US (4) US10626138B2 (sr)
EP (3) EP3656781A1 (sr)
JP (4) JP2016531122A (sr)
AU (4) AU2014306271A1 (sr)
CA (1) CA2920527A1 (sr)
DK (1) DK3041854T3 (sr)
EA (2) EA202191796A2 (sr)
ES (1) ES2773547T3 (sr)
HR (1) HRP20200250T1 (sr)
HU (1) HUE048710T2 (sr)
LT (1) LT3041854T (sr)
PL (1) PL3041854T3 (sr)
PT (1) PT3041854T (sr)
RS (1) RS59991B1 (sr)
SI (1) SI3041854T1 (sr)
WO (1) WO2015021432A1 (sr)

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CA2931547A1 (en) 2013-12-09 2015-06-18 Durect Corporation Pharmaceutically active agent complexes, polymer complexes, and compositions and methods involving the same
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WO2017106767A1 (en) 2015-12-18 2017-06-22 The Scripps Research Institute Production of unnatural nucleotides using a crispr/cas9 system
PL415967A1 (pl) * 2016-01-29 2017-07-31 Univ Warszawski 5'-tiofosforanowe analogi końca 5' mRNA (kapu), sposób ich otrzymywania i zastosowanie
WO2017223528A1 (en) 2016-06-24 2017-12-28 The Scripps Research Institute Novel nucleoside triphosphate transporter and uses thereof
MA49716A (fr) * 2017-07-11 2021-04-07 Scripps Research Inst Incorporation de nucléotides non naturels et procédés d'utilisationin vivo
AR112756A1 (es) * 2017-07-11 2019-12-11 Synthorx Inc Incorporación de nucleótidos no naturales, y su método
EP3661955A4 (en) 2017-08-03 2021-05-26 Synthorx, Inc. CYTOKINE CONJUGATES FOR THE TREATMENT OF AUTOIMMUNE DISEASES
CN114949240A (zh) 2019-02-06 2022-08-30 新索思股份有限公司 Il-2缀合物及其使用方法
CN110478950B (zh) * 2019-05-14 2021-08-06 江苏四新科技应用研究所股份有限公司 一种用于透明液体洗涤剂的消泡剂
JP2022538784A (ja) * 2019-06-14 2022-09-06 ザ スクリプス リサーチ インスティテュート 半合成生物における複製、転写および翻訳のための試薬ならびに方法
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CN115038789A (zh) 2019-12-02 2022-09-09 塑造治疗公司 治疗性编辑
WO2021137844A1 (en) * 2019-12-30 2021-07-08 Dna Syn Tech Inc. Apparatus and method for preparing nucleic acid sequences using enzyme
KR20220104058A (ko) * 2019-12-30 2022-07-25 위안디안 바이오랩스 컴퍼니 리미티드 효소를 이용한 핵산 서열 제조 방법
CA3183834A1 (en) 2020-06-25 2021-12-30 Giovanni Abbadessa Immuno oncology combination therapy with il-2 conjugates and anti-egfr antibodies
BR112023006364A2 (pt) 2020-10-09 2023-05-09 Synthorx Inc Terapia de combinação de imuno-oncologia com conjugados de il-2 e pembrolizumabe
WO2022076859A1 (en) 2020-10-09 2022-04-14 Synthorx, Inc. Immuno oncology therapies with il-2 conjugates
EP4261285A1 (en) * 2020-12-08 2023-10-18 Yudai Tabuchi Neutralizable covalent drug
WO2022123947A1 (ja) * 2020-12-08 2022-06-16 雄大 田淵 中和可能コバレントドラッグ
EP4291243A1 (en) 2021-02-12 2023-12-20 Synthorx, Inc. Lung cancer combination therapy with il-2 conjugates and an anti-pd-1 antibody or antigen-binding fragment thereof
WO2022174101A1 (en) 2021-02-12 2022-08-18 Synthorx, Inc. Skin cancer combination therapy with il-2 conjugates and cemiplimab
WO2022256538A1 (en) 2021-06-03 2022-12-08 Synthorx, Inc. Head and neck cancer combination therapy comprising an il-2 conjugate and cetuximab
WO2023288111A2 (en) 2021-07-16 2023-01-19 Aptah Bio, Inc. Polynucleotide compositions and methods for gene expression regulation
CN113759107A (zh) * 2021-08-18 2021-12-07 苏州立禾生物医学工程有限公司 生物素标记缓冲液
CN114196714B (zh) * 2021-11-04 2023-09-29 华南理工大学 利用末端脱氧核糖核苷酸转移酶无模板合成含非天然碱基寡核苷酸链的方法及其应用
CN114031648A (zh) * 2021-11-08 2022-02-11 河南师范大学 一类非天然碱基三磷酸及其合成方法和应用
CN114249788B (zh) * 2021-12-17 2023-08-08 河南师范大学 非天然碱基核苷酸磷酸单酯类前药分子及其制备方法和应用
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