MX342195B - Nucleótidos de terminación de rápida fotodescomposición 5-metoxi, 3' -oh no bloqueados y métodos para secuenciación de ácido nucleico. - Google Patents

Nucleótidos de terminación de rápida fotodescomposición 5-metoxi, 3' -oh no bloqueados y métodos para secuenciación de ácido nucleico.

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Publication number
MX342195B
MX342195B MX2014003075A MX2014003075A MX342195B MX 342195 B MX342195 B MX 342195B MX 2014003075 A MX2014003075 A MX 2014003075A MX 2014003075 A MX2014003075 A MX 2014003075A MX 342195 B MX342195 B MX 342195B
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Mexico
Prior art keywords
methoxy
methods
unblocked
fast
nucleic acid
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MX2014003075A
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English (en)
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MX2014003075A (es
Inventor
Philip Stupi Brian
Li Hong
Wu Weidong
N Hersh Megan
Hertzog David
E Morris Sidney
L Metzker Michael
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Lasergen Inc
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Publication of MX2014003075A publication Critical patent/MX2014003075A/es
Publication of MX342195B publication Critical patent/MX342195B/es

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    • C12Q1/6869—Methods for sequencing
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    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
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    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
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    • C07—ORGANIC CHEMISTRY
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    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
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    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
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    • C12Q2600/00—Oligonucleotides characterized by their use
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

La presente invención se refiere generalmente a nucleótidos y nucleósidos 3'- OH marcados y no marcados con grupos de terminación que se pueden fotodescomponerse basados en nitrobencilo substituidos con 5-metoxi para usarse en métodos y sistemas relacionados con secuenciación y análisis de ADN y ARN. Estos compuestos pueden ser utilizados como terminadores reversibles ya que exhiben una rápida cinética de incorporación de nucleótido, terminación de base simple, alta selectividad de nucleótido y rápida descomposición de grupo de terminación que da como resultado un nucleótido de origen natural.
MX2014003075A 2011-09-13 2012-09-13 Nucleótidos de terminación de rápida fotodescomposición 5-metoxi, 3' -oh no bloqueados y métodos para secuenciación de ácido nucleico. MX342195B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161534347P 2011-09-13 2011-09-13
US201161627211P 2011-10-07 2011-10-07
PCT/US2012/055231 WO2013040257A1 (en) 2011-09-13 2012-09-13 5-methoxy. 3'-oh unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing

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MX2014003075A MX2014003075A (es) 2014-11-25
MX342195B true MX342195B (es) 2016-09-20

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US (5) US8889860B2 (es)
EP (2) EP3670523B1 (es)
JP (1) JP6196972B2 (es)
KR (1) KR102048274B1 (es)
CN (1) CN104024269B (es)
AU (1) AU2012308518B2 (es)
CA (1) CA2848049C (es)
IL (1) IL231460B (es)
MX (1) MX342195B (es)
WO (1) WO2013040257A1 (es)

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