MX342195B - Nucleótidos de terminación de rápida fotodescomposición 5-metoxi, 3' -oh no bloqueados y métodos para secuenciación de ácido nucleico. - Google Patents

Nucleótidos de terminación de rápida fotodescomposición 5-metoxi, 3' -oh no bloqueados y métodos para secuenciación de ácido nucleico.

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Publication number
MX342195B
MX342195B MX2014003075A MX2014003075A MX342195B MX 342195 B MX342195 B MX 342195B MX 2014003075 A MX2014003075 A MX 2014003075A MX 2014003075 A MX2014003075 A MX 2014003075A MX 342195 B MX342195 B MX 342195B
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Mexico
Prior art keywords
methoxy
methods
unblocked
fast
nucleic acid
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MX2014003075A
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English (en)
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MX2014003075A (es
Inventor
Philip Stupi Brian
Li Hong
Wu Weidong
N Hersh Megan
Hertzog David
E Morris Sidney
L Metzker Michael
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Lasergen Inc
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Publication of MX2014003075A publication Critical patent/MX2014003075A/es
Publication of MX342195B publication Critical patent/MX342195B/es

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    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
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    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

La presente invención se refiere generalmente a nucleótidos y nucleósidos 3'- OH marcados y no marcados con grupos de terminación que se pueden fotodescomponerse basados en nitrobencilo substituidos con 5-metoxi para usarse en métodos y sistemas relacionados con secuenciación y análisis de ADN y ARN. Estos compuestos pueden ser utilizados como terminadores reversibles ya que exhiben una rápida cinética de incorporación de nucleótido, terminación de base simple, alta selectividad de nucleótido y rápida descomposición de grupo de terminación que da como resultado un nucleótido de origen natural.
MX2014003075A 2011-09-13 2012-09-13 Nucleótidos de terminación de rápida fotodescomposición 5-metoxi, 3' -oh no bloqueados y métodos para secuenciación de ácido nucleico. MX342195B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161534347P 2011-09-13 2011-09-13
US201161627211P 2011-10-07 2011-10-07
PCT/US2012/055231 WO2013040257A1 (en) 2011-09-13 2012-09-13 5-methoxy. 3'-oh unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing

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MX2014003075A MX2014003075A (es) 2014-11-25
MX342195B true MX342195B (es) 2016-09-20

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US (5) US8889860B2 (es)
EP (2) EP3670523B1 (es)
JP (1) JP6196972B2 (es)
KR (1) KR102048274B1 (es)
CN (1) CN104024269B (es)
AU (1) AU2012308518B2 (es)
CA (1) CA2848049C (es)
HK (1) HK1200838A1 (es)
IL (1) IL231460B (es)
MX (1) MX342195B (es)
WO (1) WO2013040257A1 (es)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3670523B1 (en) 2011-09-13 2021-08-11 Agilent Technologies, Inc. 3'-oh unblocked, fast photocleavable terminating nucleotides and their use in methods for nucleic acid sequencing
US10683536B2 (en) 2013-04-02 2020-06-16 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US11331643B2 (en) 2013-04-02 2022-05-17 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US11384377B2 (en) 2013-04-02 2022-07-12 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
CN107109452A (zh) * 2014-08-18 2017-08-29 分子组装公司 合成核酸的方法和设备
BR112018002525A2 (pt) 2015-08-06 2018-09-25 Lasergen Inc detecção de antígenos usando marcadores fotocliváveis
US11085076B2 (en) 2015-09-28 2021-08-10 The Trustees Of Columbia University In The City Of New York Synthesis of novel disulfide linker based nucleotides as reversible terminators for DNA sequencing by synthesis
WO2017079489A1 (en) * 2015-11-04 2017-05-11 Scepter Incorporated Atmospheric sensor network and analytical information system related thereto
US10336785B2 (en) * 2015-11-06 2019-07-02 Qiagen Sciences, Llc Methods for synthesizing nucleotide analogues with disulfide linkers
WO2017087823A1 (en) 2015-11-18 2017-05-26 Mir Kalim U Super-resolution sequencing
KR102254451B1 (ko) 2016-04-22 2021-05-20 옴니옴 인코포레이티드 뉴클레오타이드-특이적 삼원 복합체 형성의 향상된 검출을 사용하는 핵산 서열 결정 방법 및 시스템
AU2017258619B2 (en) 2016-04-29 2020-05-14 Pacific Biosciences Of California, Inc. Sequencing method employing ternary complex destabilization to identify cognate nucleotides
JP6828140B2 (ja) 2016-08-15 2021-02-10 オムニオム インコーポレイテッドＯｍｎｉｏｍｅ， Ｉｎｃ． 核酸をシーケンシングするための方法及びシステム
WO2018034780A1 (en) 2016-08-15 2018-02-22 Omniome, Inc. Sequencing method for rapid identification and processing of cognate nucleotide pairs
WO2018064297A1 (en) * 2016-09-29 2018-04-05 Ultima Genomics, Inc. Systems and methods for nucleic acid sequencing
WO2018125759A1 (en) 2016-12-30 2018-07-05 Omniome, Inc. Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides
EP3568490B1 (en) 2017-01-10 2021-03-10 Omniome, Inc. Polymerases engineered to reduce nucleotide-independent dna binding
CA3050695C (en) 2017-01-20 2024-02-20 Omniome, Inc. Process for cognate nucleotide detection in a nucleic acid sequencing workflow
SG11201906567YA (en) 2017-01-20 2019-08-27 Omniome Inc Allele-specific capture of nucleic acids
SG11201906569XA (en) 2017-01-20 2019-08-27 Omniome Inc Genotyping by polymerase binding
WO2018152162A1 (en) 2017-02-15 2018-08-23 Omniome, Inc. Distinguishing sequences by detecting polymerase dissociation
EP3596099A4 (en) 2017-03-06 2020-12-09 Singular Genomics Systems, Inc. NUCLEIC ACID SYNTHETIC SEQUENCING (SBS) METHODS COMBINING SBS CYCLE STEPS
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US9951385B1 (en) 2017-04-25 2018-04-24 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
WO2019156910A1 (en) 2018-02-06 2019-08-15 Omniome, Inc. Compositions and techniques for nucleic acid primer extension
CN110343612B (zh) * 2018-04-08 2021-07-13 上海交通大学 基于多色荧光可逆终止核苷酸的dna单分子测序系统与装置
US10400272B1 (en) 2018-04-26 2019-09-03 Omniome, Inc. Methods and compositions for stabilizing nucleic acid-nucleotide-polymerase complexes
US11339428B2 (en) 2018-05-31 2022-05-24 Pacific Biosciences Of California, Inc. Increased signal to noise in nucleic acid sequencing
US11180794B2 (en) 2018-05-31 2021-11-23 Omniome, Inc. Methods and compositions for capping nucleic acids
CA3107165A1 (en) 2018-07-24 2020-01-30 Omniome, Inc. Serial formation of ternary complex species
EP3850110A4 (en) 2018-09-11 2022-06-08 Singular Genomics Systems, Inc. MODIFIED ARCHAEA FAMILY B POLYMERASES
US10731141B2 (en) 2018-09-17 2020-08-04 Omniome, Inc. Engineered polymerases for improved sequencing
US20230028359A1 (en) 2018-10-25 2023-01-26 Singular Genomics Systems, Inc. Nucleotide analogues
US20240011086A1 (en) 2018-11-15 2024-01-11 Omniome, Inc. Electronic detection of nucleic acid structure
US10710076B2 (en) 2018-12-04 2020-07-14 Omniome, Inc. Mixed-phase fluids for nucleic acid sequencing and other analytical assays
WO2020132487A1 (en) * 2018-12-20 2020-06-25 Life Technologies Corporation Modified rhodamine dye and use thereof in biological assays
CN113227348A (zh) 2018-12-20 2021-08-06 欧姆尼欧美公司 用于分析核酸和其他分析物的温度控制
WO2020146312A1 (en) 2019-01-07 2020-07-16 Agilent Technologies, Inc. Compositions and methods for genomic dna and gene expression analysis in single cells
WO2020146397A1 (en) 2019-01-08 2020-07-16 Singular Genomics Systems, Inc. Nucleotide cleavable linkers and uses thereof
US11499189B2 (en) 2019-02-14 2022-11-15 Pacific Biosciences Of California, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
TW202124406A (zh) 2019-09-10 2021-07-01 美商歐姆尼歐美公司 核苷酸之可逆修飾
US11512295B2 (en) 2019-09-12 2022-11-29 Singular Genomics Systems, Inc. Modified thermoccocus polymerases
EP4045683A1 (en) 2019-10-18 2022-08-24 Omniome, Inc. Methods and compositions for capping nucleic acids
US20210139867A1 (en) 2019-11-08 2021-05-13 Omniome, Inc. Engineered polymerases for improved sequencing by binding
DE202019106694U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
DE202019106695U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
US11034942B1 (en) 2020-02-27 2021-06-15 Singular Genomics Systems, Inc. Modified pyrococcus polymerases and uses thereof
CN115516104A (zh) 2020-03-03 2022-12-23 加利福尼亚太平洋生物科学股份有限公司 用于对双链核酸进行测序的方法和组合物
CN115997030A (zh) 2020-04-30 2023-04-21 德迈逊科技有限公司 用于大分子操作的装置和方法
CN115836135A (zh) 2020-05-05 2023-03-21 加利福尼亚太平洋生物科学股份有限公司 用于修饰聚合酶-核酸复合物的组合物和方法
WO2021226327A1 (en) * 2020-05-08 2021-11-11 Singular Genomics Systems, Inc. Nucleotide cleavable linkers with rigid spacers and uses thereof
CN113341058A (zh) * 2021-06-09 2021-09-03 南京溯远基因科技有限公司 一种三色高通量测序试剂及测序方法
WO2023277953A1 (en) 2021-06-29 2023-01-05 Agilent Technologies, Inc. Polymerase mutants and use with 3'-oh unblocked reversible terminators
WO2023282245A1 (ja) * 2021-07-05 2023-01-12 国立研究開発法人科学技術振興機構 ヌクレオチド類の精製方法及びヌクレオチド類の精製装置並びに疎水性試薬及び疎水性基質
US20240084378A1 (en) 2022-05-11 2024-03-14 10X Genomics, Inc. Compositions and methods for in situ sequencing

Family Cites Families (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3859045A (en) 1971-07-22 1975-01-07 Allied Chem Ethoxylated aliphatic tertiary amines to reduce ozone attack on dyes in polyamide fibers
DE2136624A1 (de) 1971-07-22 1973-02-08 Boehringer Mannheim Gmbh N (6)-substituierte adenosin-derivate und verfahren zur herstellung derselben
US3822996A (en) 1972-05-22 1974-07-09 Allied Chem Water-soluble thioureas to inhibit ozone fading of dyed polyamides
US3917499A (en) 1973-10-11 1975-11-04 Dayco Corp Method of bonding components made of thermoplastic materials
US3917449A (en) 1974-02-11 1975-11-04 Allied Chem Polythioureas to inhibit ozone fading of dyed polyamides
US4304568A (en) 1980-05-19 1981-12-08 Akzona Incorporated Minimizing ozone fading in dyed polyamides by treating same with substituted piperidine thioureas
US4439356A (en) 1981-03-03 1984-03-27 Syva Company Unsymmetrical fluorescein derivatives
US4477556A (en) * 1982-08-18 1984-10-16 E. I. Du Pont De Nemours And Company Acidic o-nitroaromatics as photoinhibitors of polymerization in positive working films
US4631066A (en) 1983-07-26 1986-12-23 Teijin Limited Method for improving light-resistance of aromatic polyamide fibers
US5151507A (en) 1986-07-02 1992-09-29 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
JP2527340B2 (ja) 1986-12-15 1996-08-21 アプライド バイオシステムズ インコーポレイテッド ロ―ダミン染料の５−及び６−スクシニミジルカルボキシレ―ト異性体
US4737155A (en) 1987-06-05 1988-04-12 The Goodyear Tire & Rubber Company Stabilizers for improving the ozone fastness of dyes with oxadiazine-4-thione or triazine-4-thione compounds
USRE38416E1 (en) 1988-09-28 2004-02-03 Epoch Biosciences, Inc. Cross-linking oligonucleotides
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US5366860A (en) 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US5188934A (en) 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
AU7331094A (en) 1993-07-13 1995-02-13 United States Of America, As Represented By The Secretary, Department Of Health And Human Services, The A3 adenosine receptor agonists
GB9315847D0 (en) 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
DE69416317T2 (de) * 1993-11-19 1999-09-09 Equinap Pty Ltd Harn- und kotfänger für equiden
US5808045A (en) 1994-09-02 1998-09-15 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
US5872244A (en) 1994-09-02 1999-02-16 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US6214987B1 (en) 1994-09-02 2001-04-10 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent formation of phosphodiester bonds using protected nucleotides
US5763594A (en) 1994-09-02 1998-06-09 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US5684142A (en) 1995-06-07 1997-11-04 Oncor, Inc. Modified nucleotides for nucleic acid labeling
US5728529A (en) 1995-06-23 1998-03-17 Baylor College Of Medicine Alternative dye-labeled ribonucleotides, deoxyribonucleotides, and dideoxyribonucleotides for automated DNA analysis
US5994063A (en) 1995-06-23 1999-11-30 Metzker; Michael L. Substituted 4,4-difluoro-4-bora-3A,4A-diaza-s-indacene compounds for homogenous amplification/detection assays
US5861287A (en) 1995-06-23 1999-01-19 Baylor College Of Medicine Alternative dye-labeled primers for automated DNA sequencing
US5614386A (en) 1995-06-23 1997-03-25 Baylor College Of Medicine Alternative dye-labeled primers for automated DNA sequencing
EP0833936A4 (en) 1995-06-23 2005-03-02 Baylor College Medicine ALTERNATIVE DYE-MARKED PRIMERS, RIBONUCLEOTIDES, DEOXYRIBONUCLEOTIDES AND DIDEOXYRIBONUCLEOTIDES FOR AUTOMATIC DNA ANALYSIS AND HOMOGENEOUS AMPLIFICATION / DETECTION DETECTION
US6657052B1 (en) 1997-04-11 2003-12-02 University Of Arkansas Biomolecular labeling
US6211165B1 (en) 1997-05-09 2001-04-03 The Trustees Of The University Of Pennsylvania Methods and compositions for reducing ischemic injury of the heart by administering adenosine receptor agonists and antagonists
GB9727186D0 (en) 1997-12-24 1998-02-25 Du Pont Uk Photoactive materials applicable to imaging systems
WO1999049082A2 (en) 1998-03-23 1999-09-30 Invitrogen Corporation Modified nucleotides and methods useful for nucleic acid sequencing
US6232075B1 (en) 1998-12-14 2001-05-15 Li-Cor, Inc. Heterogeneous assay for pyrophosphate detection
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
EP1218543A2 (en) 1999-09-29 2002-07-03 Solexa Ltd. Polynucleotide sequencing
US6586413B2 (en) 1999-11-05 2003-07-01 The United States Of America As Represented By The Department Of Health And Human Services Methods and compositions for reducing ischemic injury of the heart by administering adenosine receptor agonists and antagonists
EP1294735A2 (en) 2000-05-26 2003-03-26 Novirio Pharmaceuticals Limited Methods and compositions for treating flaviviruses and pestiviruses
US6869764B2 (en) 2000-06-07 2005-03-22 L--Cor, Inc. Nucleic acid sequencing using charge-switch nucleotides
MY141594A (en) 2000-06-15 2010-05-14 Novirio Pharmaceuticals Ltd 3'-PRODRUGS OF 2'-DEOXY-ß-L-NUCLEOSIDES
EP1975251A3 (en) 2000-07-07 2009-03-25 Visigen Biotechnologies, Inc. Real-time sequence determination
US7125660B2 (en) 2000-09-13 2006-10-24 Archemix Corp. Nucleic acid sensor molecules and methods of using same
EP3034627B1 (en) 2000-10-06 2019-01-30 The Trustees of Columbia University in the City of New York Massive parallel method for decoding dna and rna
DK1410023T3 (da) 2001-04-10 2006-10-09 Ecole Polytech Fremgangsmåder til anvendelse af 06-alkylguanin-DNA-alkyltransferaser
EP1401851B8 (de) 2001-07-03 2008-09-10 febit holding GmbH Zweistufige schutzgruppen für die synthese von biopolymeren
US6995841B2 (en) 2001-08-28 2006-02-07 Rice University Pulsed-multiline excitation for color-blind fluorescence detection
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
WO2003066812A2 (en) 2002-02-05 2003-08-14 Baylor College Of Medecine Substituted 4,4-difluoro-4-bora-3a, 4a-diaza-s-indacene compounds for 8-color dna sequencing
AU2003224836A1 (en) 2002-04-12 2003-10-27 Stratagene Dual-labeled nucleotides
JP4014142B2 (ja) 2002-05-02 2007-11-28 独立行政法人科学技術振興機構 光分解性シランカップリング剤
JP2005536747A (ja) 2002-08-21 2005-12-02 データスコープ・インヴェストメント・コーポレイション 保護コーティングをアッセイ基質に形成する為の方法と組成物及び、これにより生産された基質
ES2407681T3 (es) 2002-08-23 2013-06-13 Illumina Cambridge Limited Nucleótidos modificados para la secuenciación de polinucleótidos.
DK3147292T3 (en) 2002-08-23 2018-12-03 Illumina Cambridge Ltd LABELED NUCLEOTIDES
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
CZ294538B6 (cs) 2002-12-30 2005-01-12 Ústav Experimentální Botaniky Akademie Vědčeské Re Substituční deriváty N6-benzyladenosinu, způsob jejich přípravy, jejich použití pro přípravu léčiv, kosmetických přípravků a růstových regulátorů, farmaceutické přípravky, kosmetické přípravky a růstové regulátory tyto sloučeniny obsahující
US7427679B2 (en) 2003-08-30 2008-09-23 Agilent Technologies, Inc. Precursors for two-step polynucleotide synthesis
US7193077B2 (en) 2003-08-30 2007-03-20 Agilent Technologies, Inc. Exocyclic amine triaryl methyl protecting groups in two-step polynucleotide synthesis
US7417139B2 (en) 2003-08-30 2008-08-26 Agilent Technologies, Inc. Method for polynucleotide synthesis
EP1725572B1 (de) 2003-11-05 2017-05-31 AGCT GmbH Makromolekulare nukleotidverbindungen und methoden zu deren anwendung
EP2436778A3 (en) 2004-03-03 2012-07-11 The Trustees of Columbia University in the City of New York Photocleavable fluorescent nucleotides for DNA sequencing on chip constructed by site-specific coupling chemistry
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
EP3373174A1 (en) * 2006-03-31 2018-09-12 Illumina, Inc. Systems and devices for sequence by synthesis analysis
US7897737B2 (en) * 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
JP5513122B2 (ja) * 2006-12-05 2014-06-04 レーザーゲン インコーポレイテッド 光開裂性標識ヌクレオチドとヌクレオシドおよび標識ヌクレオチドとヌクレオシドならびにｄｎａ配列決定におけるそれらの使用法
US7893227B2 (en) 2006-12-05 2011-02-22 Lasergen, Inc. 3′-OH unblocked nucleotides and nucleosides base modified with non-cleavable, terminating groups and methods for their use in DNA sequencing
US8481259B2 (en) * 2007-02-05 2013-07-09 Intelligent Bio-Systems, Inc. Methods and devices for sequencing nucleic acids in smaller batches
US7556537B2 (en) 2007-11-15 2009-07-07 Gm Global Technology Operations, Inc. Solenoid and connector assembly
JP5385538B2 (ja) 2008-03-10 2014-01-08 学校法人神奈川大学 ｏ−ニトロベンジル基含有シラザン化合物、その製造方法及び用途
CN103588839B (zh) 2008-06-11 2017-04-12 激光基因公司 核苷酸和核苷以及将其用于dna测序的方法
JP5446793B2 (ja) 2009-12-04 2014-03-19 大日本印刷株式会社 感光性樹脂組成物、およびこれを用いた物品、及びネガ型パターン形成方法
JP2011116869A (ja) 2009-12-04 2011-06-16 San Apro Kk 光塩基発生剤
US9005565B2 (en) 2010-06-24 2015-04-14 Hamid-Reza Jahangiri-Famenini Method and apparatus for forming graphene
WO2012037394A1 (en) 2010-09-16 2012-03-22 Ibis Biosciences, Inc. Stabilization of ozone-labile fluorescent dyes by thiourea
EP3670523B1 (en) 2011-09-13 2021-08-11 Agilent Technologies, Inc. 3'-oh unblocked, fast photocleavable terminating nucleotides and their use in methods for nucleic acid sequencing

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