MX338089B - Nucleotidos y necleosidos marcados de foto-escision y necleotidos y necleosidos marcados y metodos para su uso en secuenciacion de adn. - Google Patents

Nucleotidos y necleosidos marcados de foto-escision y necleotidos y necleosidos marcados y metodos para su uso en secuenciacion de adn.

Info

Publication number
MX338089B
MX338089B MX2011013323A MX2011013323A MX338089B MX 338089 B MX338089 B MX 338089B MX 2011013323 A MX2011013323 A MX 2011013323A MX 2011013323 A MX2011013323 A MX 2011013323A MX 338089 B MX338089 B MX 338089B
Authority
MX
Mexico
Prior art keywords
nucleosides
group
labeled nucleotides
photocleavable
dna
Prior art date
Application number
MX2011013323A
Other languages
English (en)
Inventor
Vladislav A Litosh
Brian P Stupi
Michael L Metzker
Weidong Wu
Original Assignee
Lasergen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/567,193 external-priority patent/US7893227B2/en
Priority claimed from US11/567,189 external-priority patent/US7897737B2/en
Application filed by Lasergen Inc filed Critical Lasergen Inc
Publication of MX338089B publication Critical patent/MX338089B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

La presente invención se refiere a un compuesto de acuerdo con la siguiente Fórmula o una sal del mismo: en donde R1 es H, monofosfato, difosfato, trifosfato, o alfa tiotrifosfato; R2 es H u OH; la base es citosina, uracilo, timina. Adenina, guanina, o su derivado de existencia natural, la porción de terminación de Escisión-Enlazdor-Colorante es un grupo de la Fórmula: en donde R3 y R4 son cada uno independientemente H, en alquilo de cadena lineal o ramificada de 1 a 12 átomos de carbono, un alquenilo o polienilo de cadena lineal o ramificada de 2 a 12 átomos de carbono, un alquinilo o polialquinilo de cadena lineal o ramificada de 2 a 12 átomos de carbono o un grupo aromático, con la condición de que al menos uno de R3 y R4 es H; R5, R6, R7 y R8 son cada uno independientes H, OCH3, NO2, CN, un haluro, un alquilo de cadena lineal o ramificada de 1 a 12 átomos de carbono, un alquenilo o polienilo de cadena lineal o ramificada de 2 a 12 átomos de carbono, un alquinilo o polialquinilo de cadena lineal o ramificada de 2 a 12 átomos de carbono, un grupo aromático, o el grupo Enlazador-Colorante, con la condición de que al menos uno de R5, R6, R7 y R8 es el grupo Enlazador-Colorante; la porción de terminación de escisión que imparte propiedades de terminación de la polimerasa para el compuesto y está unido a la base mediante un enlace directo o un grupo enlazador; el enlace y el grupo enlazador son, independientemente, un grupo bifuncional; y el Colorante es un fluoróforo.
MX2011013323A 2006-12-05 2007-12-05 Nucleotidos y necleosidos marcados de foto-escision y necleotidos y necleosidos marcados y metodos para su uso en secuenciacion de adn. MX338089B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11/567,193 US7893227B2 (en) 2006-12-05 2006-12-05 3′-OH unblocked nucleotides and nucleosides base modified with non-cleavable, terminating groups and methods for their use in DNA sequencing
US11/567,189 US7897737B2 (en) 2006-12-05 2006-12-05 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
PCT/US2007/086559 WO2008070749A2 (en) 2006-12-05 2007-12-05 Photocleavable labeled nucleotides and nucleosides and labeled nucleotides and nucleosides and methods for their use in dna sequencing

Publications (1)

Publication Number Publication Date
MX338089B true MX338089B (es) 2016-04-01

Family

ID=39493724

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2011013323A MX338089B (es) 2006-12-05 2007-12-05 Nucleotidos y necleosidos marcados de foto-escision y necleotidos y necleosidos marcados y metodos para su uso en secuenciacion de adn.
MX2009005936A MX2009005936A (es) 2006-12-05 2007-12-05 Nucleotidos y nucleosidos marcados de foto-escision y nucleotidos y nucleosidos marcados y metodos para su uso en secunciacion de adn.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2009005936A MX2009005936A (es) 2006-12-05 2007-12-05 Nucleotidos y nucleosidos marcados de foto-escision y nucleotidos y nucleosidos marcados y metodos para su uso en secunciacion de adn.

Country Status (12)

Country Link
EP (1) EP2125856B1 (es)
JP (1) JP5513122B2 (es)
KR (1) KR101504640B1 (es)
CN (1) CN102443030B (es)
AU (1) AU2007329361B2 (es)
BR (1) BRPI0719923B1 (es)
CA (1) CA2670937C (es)
IL (1) IL199051A (es)
MX (2) MX338089B (es)
NZ (1) NZ577303A (es)
RU (1) RU2009121089A (es)
WO (1) WO2008070749A2 (es)

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* Cited by examiner, † Cited by third party
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US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
EA201100001A3 (ru) 2008-06-11 2013-03-29 Лейзеджен, Инк. Нуклеотиды и нуклеозиды и методы их использования в секвенировании днк
US10759824B2 (en) 2008-09-03 2020-09-01 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags
WO2017120148A1 (en) 2016-01-04 2017-07-13 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags
EP2321429B1 (en) 2008-09-03 2016-08-17 Quantumdx Group Limited Methods and kits for nucleic acid sequencing
MX342195B (es) 2011-09-13 2016-09-20 Lasergen Inc Nucleótidos de terminación de rápida fotodescomposición 5-metoxi, 3' -oh no bloqueados y métodos para secuenciación de ácido nucleico.
BR112014016846B1 (pt) 2012-01-13 2020-09-29 Koninklijke Philips N.V Dispositivo para o controle óptico de uma reação em etapas iterativa para determinar uma sequência de um ácido nucleico por síntese e método para o controle óptico de uma reação em etapas iterativa para determinar uma sequência de um ácido nucleico por síntese
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CN105555966B (zh) * 2013-07-31 2020-09-25 株式会社日立高新技术 核酸分析用流动池和核酸分析装置
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CN105001292A (zh) * 2015-07-14 2015-10-28 深圳市瀚海基因生物科技有限公司 一种光可断裂的荧光标记可逆终端化合物及其在dna或rna测序中的用途
GB201711219D0 (en) 2017-07-12 2017-08-23 Illumina Cambridge Ltd Short pendant arm linkers for nucleotides in sequencing applications
CA3125920A1 (en) 2019-01-07 2020-07-16 Agilent Technologies, Inc. Compositions and methods for genomic dna and gene expression analysis in single cells
CN110568039B (zh) * 2019-09-06 2021-05-04 中国科学院生物物理研究所 使用新型电化学传感器的氨基酸特异实时检测方法
WO2021194640A1 (en) * 2020-03-26 2021-09-30 Agilent Technologies, Inc. Methods and compositions for multistage primer extension reactions
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EP4355757A1 (en) * 2021-06-17 2024-04-24 F. Hoffmann-La Roche AG Nucleoside-5 -oligophosphates having a cationically-modified nucleobase
WO2023277953A1 (en) * 2021-06-29 2023-01-05 Agilent Technologies, Inc. Polymerase mutants and use with 3'-oh unblocked reversible terminators
CN114250283B (zh) * 2021-10-15 2022-10-04 深圳铭毅智造科技有限公司 基于环境敏感染料的单色荧光mrt基因测序试剂及方法

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Also Published As

Publication number Publication date
AU2007329361A1 (en) 2008-06-12
EP2125856A2 (en) 2009-12-02
EP2125856A4 (en) 2013-12-25
JP2011506265A (ja) 2011-03-03
BRPI0719923A2 (pt) 2014-03-04
EP2125856B1 (en) 2016-09-14
AU2007329361B2 (en) 2013-07-18
BRPI0719923B1 (pt) 2021-08-17
MX2009005936A (es) 2010-01-15
CA2670937A1 (en) 2008-06-12
CN102443030A (zh) 2012-05-09
WO2008070749A2 (en) 2008-06-12
CN102443030B (zh) 2015-12-16
RU2009121089A (ru) 2011-01-20
WO2008070749A3 (en) 2008-11-27
KR20090101451A (ko) 2009-09-28
HK1169413A1 (zh) 2013-01-25
NZ577303A (en) 2011-12-22
JP5513122B2 (ja) 2014-06-04
CA2670937C (en) 2016-07-26
IL199051A (en) 2013-11-28
KR101504640B1 (ko) 2015-03-20

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