BRPI0915173B8 - compostos de nucleotídeos e nucleosídeos e método de sequenciamento de um ácido nucleico-alvo - Google Patents

compostos de nucleotídeos e nucleosídeos e método de sequenciamento de um ácido nucleico-alvo

Info

Publication number
BRPI0915173B8
BRPI0915173B8 BRPI0915173A BRPI0915173A BRPI0915173B8 BR PI0915173 B8 BRPI0915173 B8 BR PI0915173B8 BR PI0915173 A BRPI0915173 A BR PI0915173A BR PI0915173 A BRPI0915173 A BR PI0915173A BR PI0915173 B8 BRPI0915173 B8 BR PI0915173B8
Authority
BR
Brazil
Prior art keywords
sequencing
nucleotide
nucleic acid
target nucleic
nucleoside compounds
Prior art date
Application number
BRPI0915173A
Other languages
English (en)
Inventor
P Stupi Brian
N Hersh Megan
L Metzker Michael
A Litosh Vladislav
Wu Weidong
Original Assignee
Agilent Technologies Inc
Lasergen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agilent Technologies Inc, Lasergen Inc filed Critical Agilent Technologies Inc
Publication of BRPI0915173A2 publication Critical patent/BRPI0915173A2/pt
Publication of BRPI0915173B1 publication Critical patent/BRPI0915173B1/pt
Publication of BRPI0915173B8 publication Critical patent/BRPI0915173B8/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/14—Pyrrolo-pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/117—Modifications characterised by incorporating modified base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Pyrane Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

composto de nucleotídeos e nucleosídeos e método para uso dos mesmos em sequenciamento de dna. a presente invenção refere-se, de modo geral, a grupos terminais cliváveis rotulados e não rotulados e métodos para sequenciamento de dna e outros tipos de análise de dna. mais particularmente, a invenção refere-se, em parte, a nucleotídeos e nucleosídeos com grupos quimicamente cliváveis, fotocliváveis, enzimaticamente cliváveis ou não fotocliváveis e métodos para seu uso em sequenciamento de dna e sua aplicação em pesquisa biomédica.
BRPI0915173A 2008-06-11 2009-06-11 compostos de nucleotídeos e nucleosídeos e método de sequenciamento de um ácido nucleico-alvo BRPI0915173B8 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US6079508P 2008-06-11 2008-06-11
US61/060,795 2008-06-11
US18477909P 2009-06-05 2009-06-05
US61/184,779 2009-06-05
PCT/US2009/047071 WO2009152353A2 (en) 2008-06-11 2009-06-11 Nucleotides and nucleosides and methods for their use in dna sequencing

Publications (3)

Publication Number Publication Date
BRPI0915173A2 BRPI0915173A2 (pt) 2015-08-18
BRPI0915173B1 BRPI0915173B1 (pt) 2021-05-04
BRPI0915173B8 true BRPI0915173B8 (pt) 2021-05-25

Family

ID=41417396

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0915173A BRPI0915173B8 (pt) 2008-06-11 2009-06-11 compostos de nucleotídeos e nucleosídeos e método de sequenciamento de um ácido nucleico-alvo

Country Status (15)

Country Link
US (4) US8148503B2 (pt)
EP (1) EP2307565B1 (pt)
JP (1) JP5143953B2 (pt)
KR (1) KR101677772B1 (pt)
CN (2) CN102264917B (pt)
AU (1) AU2009257369B2 (pt)
BR (1) BRPI0915173B8 (pt)
CA (2) CA2727709C (pt)
EA (1) EA201100001A3 (pt)
HK (1) HK1163184A1 (pt)
IL (1) IL209917A (pt)
MX (1) MX2010013678A (pt)
NZ (2) NZ590265A (pt)
WO (1) WO2009152353A2 (pt)
ZA (1) ZA201100122B (pt)

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IL209917A (en) 2014-06-30
US20100041041A1 (en) 2010-02-18
CN102264917A (zh) 2011-11-30
AU2009257369A1 (en) 2009-12-17
CA2727709A1 (en) 2009-12-17
KR20110031191A (ko) 2011-03-24
IL209917A0 (en) 2011-02-28
AU2009257369B2 (en) 2015-03-26
CN103588839A (zh) 2014-02-19
BRPI0915173A2 (pt) 2015-08-18
US20130035237A1 (en) 2013-02-07
KR101677772B1 (ko) 2016-11-18
US9200319B2 (en) 2015-12-01
EA201100001A3 (ru) 2013-03-29
MX2010013678A (es) 2011-02-24
US8148503B2 (en) 2012-04-03
WO2009152353A3 (en) 2013-09-26
CN102264917B (zh) 2015-06-10
EP2307565B1 (en) 2017-11-29
NZ590265A (en) 2012-11-30
EP2307565A2 (en) 2011-04-13
CA2979146A1 (en) 2009-12-17
US8497360B2 (en) 2013-07-30
CA2727709C (en) 2017-11-14
WO2009152353A2 (en) 2009-12-17
HK1163184A1 (en) 2012-09-07
US20150140559A1 (en) 2015-05-21
EA201100001A2 (ru) 2012-10-30
CN103588839B (zh) 2017-04-12
NZ594812A (en) 2012-12-21
CA2979146C (en) 2020-07-07
JP5143953B2 (ja) 2013-02-13
BRPI0915173B1 (pt) 2021-05-04
US20140051848A1 (en) 2014-02-20
US8877905B2 (en) 2014-11-04
JP2011529449A (ja) 2011-12-08
ZA201100122B (en) 2013-09-25

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