MX2009005936A - Nucleotidos y nucleosidos marcados de foto-escision y nucleotidos y nucleosidos marcados y metodos para su uso en secunciacion de adn. - Google Patents

Nucleotidos y nucleosidos marcados de foto-escision y nucleotidos y nucleosidos marcados y metodos para su uso en secunciacion de adn.

Info

Publication number
MX2009005936A
MX2009005936A MX2009005936A MX2009005936A MX2009005936A MX 2009005936 A MX2009005936 A MX 2009005936A MX 2009005936 A MX2009005936 A MX 2009005936A MX 2009005936 A MX2009005936 A MX 2009005936A MX 2009005936 A MX2009005936 A MX 2009005936A
Authority
MX
Mexico
Prior art keywords
nucleosides
group
labeled nucleotides
photocleavable
dna
Prior art date
Application number
MX2009005936A
Other languages
English (en)
Inventor
Vladislav A Litosh
Brian P Stupi
Michael L Metzker
Weidong Wu
Original Assignee
Lasergen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/567,193 external-priority patent/US7893227B2/en
Priority claimed from US11/567,189 external-priority patent/US7897737B2/en
Application filed by Lasergen Inc filed Critical Lasergen Inc
Publication of MX2009005936A publication Critical patent/MX2009005936A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Se proporcionan nucleótidos, nucleósidos, y sus derivados descritos aquí, que pueden ser usados en tecnología de secuenciación de ADN y otros tipos de análisis de ADN. En un modalidad, el nucleótido o nucleósido con el grupo 3'-OH no protegido es derivatizado en la núcleobase para incluir un colorante fluorescente unido a través de un enlazador a un grupo de terminación de no escisión. El grupo fluorescente de no escisión está diseñado para terminar la síntesis de ADN, de manera que los oligómeros de ADN pueden ser secuenciados eficientemente en un formato paralelo. En otra modalidad, el nucleótido o nucleósido con un grupo 3'-OH no protegidos es derivatizado en la núcleobase para incluir un colorante fluorescente unido a través de un enlazador a un grupo de terminación de foto-escisión. El grupo fluorescente de foto-escisión está diseñado para terminar la síntesis de ADN así como puede ser dividido de manera que los oligómeros de ADN pueden ser secuenciados eficientemente en un formato paralelo.
MX2009005936A 2006-12-05 2007-12-05 Nucleotidos y nucleosidos marcados de foto-escision y nucleotidos y nucleosidos marcados y metodos para su uso en secunciacion de adn. MX2009005936A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11/567,193 US7893227B2 (en) 2006-12-05 2006-12-05 3′-OH unblocked nucleotides and nucleosides base modified with non-cleavable, terminating groups and methods for their use in DNA sequencing
US11/567,189 US7897737B2 (en) 2006-12-05 2006-12-05 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
PCT/US2007/086559 WO2008070749A2 (en) 2006-12-05 2007-12-05 Photocleavable labeled nucleotides and nucleosides and labeled nucleotides and nucleosides and methods for their use in dna sequencing

Publications (1)

Publication Number Publication Date
MX2009005936A true MX2009005936A (es) 2010-01-15

Family

ID=39493724

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2009005936A MX2009005936A (es) 2006-12-05 2007-12-05 Nucleotidos y nucleosidos marcados de foto-escision y nucleotidos y nucleosidos marcados y metodos para su uso en secunciacion de adn.
MX2011013323A MX338089B (es) 2006-12-05 2007-12-05 Nucleotidos y necleosidos marcados de foto-escision y necleotidos y necleosidos marcados y metodos para su uso en secuenciacion de adn.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2011013323A MX338089B (es) 2006-12-05 2007-12-05 Nucleotidos y necleosidos marcados de foto-escision y necleotidos y necleosidos marcados y metodos para su uso en secuenciacion de adn.

Country Status (13)

Country Link
EP (1) EP2125856B1 (es)
JP (1) JP5513122B2 (es)
KR (1) KR101504640B1 (es)
CN (1) CN102443030B (es)
AU (1) AU2007329361B2 (es)
BR (1) BRPI0719923B1 (es)
CA (1) CA2670937C (es)
HK (1) HK1169413A1 (es)
IL (1) IL199051A (es)
MX (2) MX2009005936A (es)
NZ (1) NZ577303A (es)
RU (1) RU2009121089A (es)
WO (1) WO2008070749A2 (es)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
MX2010013678A (es) 2008-06-11 2011-02-24 Lasergen Inc Nucleotidos y nucleosidos y metodos para su uso en secuenciacion de adn.
US10759824B2 (en) 2008-09-03 2020-09-01 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags
WO2010026488A2 (en) 2008-09-03 2010-03-11 Quantumdx Group Limited Methods and kits for nucleic acid sequencing
AU2012308518B2 (en) 2011-09-13 2017-08-17 Agilent Technologies, Inc. 5-methoxy, 3'-oh unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
RU2611207C2 (ru) * 2012-01-13 2017-02-21 Конинклейке Филипс Н.В. Секвенирование днк с повторным использованием реагентов на проволочной сетке
CN103602719B (zh) * 2013-04-07 2016-06-08 北京迈基诺基因科技有限责任公司 一种基因测序方法
US10112195B2 (en) 2013-07-31 2018-10-30 Hitachi High-Technologies Corporation Flow cell for nucleic acid analysis and nucleic acid analyzer
GB201414098D0 (en) 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers
RU2582198C1 (ru) * 2014-11-20 2016-04-20 Федеральное государственное бюджетное учреждение науки Лимнологический институт Сибирского отделения Российской академии наук (ЛИН СО РАН) Аналоги природных дезоксирибонуклеозидтрифосфатов и рибонуклеозидтрифосфатов, содержащие репортёрные флуоресцентные группы, для использования в аналитической биоорганической химии
CN105001292A (zh) * 2015-07-14 2015-10-28 深圳市瀚海基因生物科技有限公司 一种光可断裂的荧光标记可逆终端化合物及其在dna或rna测序中的用途
EP3400444A4 (en) * 2016-01-04 2019-10-16 QuantuMDx Group Limited DESIGN, SYNTHESIS AND USE OF SYNTHETIC NUCLEOTIDES COMPRISING SPECIFIC CHARGE LABELS
GB201711219D0 (en) 2017-07-12 2017-08-23 Illumina Cambridge Ltd Short pendant arm linkers for nucleotides in sequencing applications
CN113272443A (zh) 2019-01-07 2021-08-17 安捷伦科技有限公司 用于单细胞中的基因组dna和基因表达分析的组合物和方法
CN110568039B (zh) * 2019-09-06 2021-05-04 中国科学院生物物理研究所 使用新型电化学传感器的氨基酸特异实时检测方法
GB2605404A (en) * 2021-03-30 2022-10-05 Sumitomo Chemical Co Sequencing method
EP4355757A1 (en) * 2021-06-17 2024-04-24 F. Hoffmann-La Roche AG Nucleoside-5 -oligophosphates having a cationically-modified nucleobase
CN114250283B (zh) * 2021-10-15 2022-10-04 深圳铭毅智造科技有限公司 基于环境敏感染料的单色荧光mrt基因测序试剂及方法

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US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
YU187991A (sh) * 1990-12-11 1994-09-09 Hoechst Aktiengesellschaft 3-(2)-amino-ali tiol-modifikovani, s fluorescentnom bojom vezani nukleozidi, nukleotidi i oligonukleotidi, postupak za njihovo dobijanje i njihova upotreba
US6211165B1 (en) * 1997-05-09 2001-04-03 The Trustees Of The University Of Pennsylvania Methods and compositions for reducing ischemic injury of the heart by administering adenosine receptor agonists and antagonists
US6586413B2 (en) * 1999-11-05 2003-07-01 The United States Of America As Represented By The Department Of Health And Human Services Methods and compositions for reducing ischemic injury of the heart by administering adenosine receptor agonists and antagonists
ATE356222T1 (de) 2000-10-06 2007-03-15 Univ Columbia Massives parallelverfahren zur dekodierung von dna und rna
AU2002251257B2 (en) * 2001-04-10 2007-08-30 Ecole Polytechnique Federale De Lausanne Methods of Using O6-alkylguanine-DNA Alkyltransferases
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CZ294538B6 (cs) * 2002-12-30 2005-01-12 Ústav Experimentální Botaniky Akademie Vědčeské Re Substituční deriváty N6-benzyladenosinu, způsob jejich přípravy, jejich použití pro přípravu léčiv, kosmetických přípravků a růstových regulátorů, farmaceutické přípravky, kosmetické přípravky a růstové regulátory tyto sloučeniny obsahující
US7622279B2 (en) * 2004-03-03 2009-11-24 The Trustees Of Columbia University In The City Of New York Photocleavable fluorescent nucleotides for DNA sequencing on chip constructed by site-specific coupling chemistry

Also Published As

Publication number Publication date
EP2125856B1 (en) 2016-09-14
KR101504640B1 (ko) 2015-03-20
JP5513122B2 (ja) 2014-06-04
BRPI0719923B1 (pt) 2021-08-17
WO2008070749A3 (en) 2008-11-27
EP2125856A4 (en) 2013-12-25
RU2009121089A (ru) 2011-01-20
JP2011506265A (ja) 2011-03-03
KR20090101451A (ko) 2009-09-28
CN102443030B (zh) 2015-12-16
BRPI0719923A2 (pt) 2014-03-04
CA2670937C (en) 2016-07-26
WO2008070749A2 (en) 2008-06-12
HK1169413A1 (zh) 2013-01-25
AU2007329361B2 (en) 2013-07-18
CN102443030A (zh) 2012-05-09
CA2670937A1 (en) 2008-06-12
AU2007329361A1 (en) 2008-06-12
MX338089B (es) 2016-04-01
EP2125856A2 (en) 2009-12-02
IL199051A (en) 2013-11-28
NZ577303A (en) 2011-12-22

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