WO2007140506A8 - Modified microbial nucleic acid for use in detection and analysis of microorganisms - Google Patents

Modified microbial nucleic acid for use in detection and analysis of microorganisms Download PDF

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Publication number
WO2007140506A8
WO2007140506A8 PCT/AU2006/000755 AU2006000755W WO2007140506A8 WO 2007140506 A8 WO2007140506 A8 WO 2007140506A8 AU 2006000755 W AU2006000755 W AU 2006000755W WO 2007140506 A8 WO2007140506 A8 WO 2007140506A8
Authority
WO
WIPO (PCT)
Prior art keywords
microorganisms
nucleic acid
analysis
detection
modified
Prior art date
Application number
PCT/AU2006/000755
Other languages
French (fr)
Other versions
WO2007140506A1 (en
Inventor
Douglas Spencer Millar
John R. Melki
Original Assignee
Human Genetic Signatures Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Human Genetic Signatures Pty Ltd filed Critical Human Genetic Signatures Pty Ltd
Priority to EP06741172A priority Critical patent/EP2021497A1/en
Priority to AU2006344331A priority patent/AU2006344331A1/en
Priority to CN200680055508A priority patent/CN101522908A/en
Priority to JP2009512366A priority patent/JP2009538603A/en
Priority to PCT/AU2006/000755 priority patent/WO2007140506A1/en
Priority to CA002654010A priority patent/CA2654010A1/en
Publication of WO2007140506A1 publication Critical patent/WO2007140506A1/en
Publication of WO2007140506A8 publication Critical patent/WO2007140506A8/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides derivatives or modified nucleic acid sequences of several microorganisms for use in the detection and analysis of said microorganisms. The derivative nucleic acids contain the bases adenosine (A), guanosine (G), T (thymine) and U (uracil or some other non-A, G or T base or base-like entity). Given that microbial nucleic acids do not contain methylated cytosine (C) or other C alterations, all C are converted to U. These sequences are amplified where the U in the derivative nucleic acid is replaced by a T, resulting in a modified sequence with the same number of total bases as the corresponding unmodified microbial nucleic acid sequence but made up of a combination of three bases only: A, G and T. As a consequence of this process the nucleic acids derived from the upper and lower strands of the original dsDNA are no longer complementary and the modified microbial sequences have reduced relative genomic complexity for use in detection and analysis of microorganisms.
PCT/AU2006/000755 2006-06-02 2006-06-02 Modified microbial nucleic acid for use in detection and analysis of microorganisms WO2007140506A1 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP06741172A EP2021497A1 (en) 2006-06-02 2006-06-02 Modified microbial nucleic acid for use in detection and analysis of microorganisms
AU2006344331A AU2006344331A1 (en) 2006-06-02 2006-06-02 Modified microbial nucleic acid for use in detection and analysis of microorganisms
CN200680055508A CN101522908A (en) 2006-06-02 2006-06-02 Modified microbial nucleic acid for use in detection and analysis of microorganisms
JP2009512366A JP2009538603A (en) 2006-06-02 2006-06-02 Microbial modified nucleic acids for use in the detection and analysis of microorganisms
PCT/AU2006/000755 WO2007140506A1 (en) 2006-06-02 2006-06-02 Modified microbial nucleic acid for use in detection and analysis of microorganisms
CA002654010A CA2654010A1 (en) 2006-06-02 2006-06-02 Modified microbial nucleic acid for use in detection and analysis of microorganisms

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/AU2006/000755 WO2007140506A1 (en) 2006-06-02 2006-06-02 Modified microbial nucleic acid for use in detection and analysis of microorganisms

Publications (2)

Publication Number Publication Date
WO2007140506A1 WO2007140506A1 (en) 2007-12-13
WO2007140506A8 true WO2007140506A8 (en) 2010-04-15

Family

ID=38800944

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AU2006/000755 WO2007140506A1 (en) 2006-06-02 2006-06-02 Modified microbial nucleic acid for use in detection and analysis of microorganisms

Country Status (6)

Country Link
EP (1) EP2021497A1 (en)
JP (1) JP2009538603A (en)
CN (1) CN101522908A (en)
AU (1) AU2006344331A1 (en)
CA (1) CA2654010A1 (en)
WO (1) WO2007140506A1 (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100092972A1 (en) * 2007-03-16 2010-04-15 Human Genetic Signatures Pty Ltd. Assay for gene expression
FR2940805B1 (en) 2009-01-05 2015-10-16 Biomerieux Sa METHOD FOR AMPLIFYING AND / OR DETECTING NUCLEIC ACIDS, KITS AND USES THEREOF
WO2010121308A1 (en) * 2009-04-23 2010-10-28 Human Genetic Signatures Pty Ltd Detection of hepatitis c virus
US8628947B2 (en) 2010-08-30 2014-01-14 Intervet Inc. Potomac horse fever isolates
US9732375B2 (en) * 2011-09-07 2017-08-15 Human Genetic Signatures Pty. Ltd. Molecular detection assay using direct treatment with a bisulphite reagent
WO2013082500A2 (en) 2011-12-02 2013-06-06 Rhode Island Hospital Vaccine for falciparum malaria
CN103173536B (en) * 2012-12-21 2014-10-08 山东出入境检验检疫局检验检疫技术中心 Kit for nicked-endonuclease-nucleic-acid-isothermal-amplification rapid detection of Escherichia coli
US9775894B2 (en) 2013-07-09 2017-10-03 University Of Washington Through Its Center For Commercialization Methods and compositions for activation of innate immune responses through RIG-I like receptor signaling
CN106103470B (en) * 2013-12-30 2020-10-27 丹尼斯科美国公司 Enhanced protein expression
US10342861B2 (en) 2014-05-27 2019-07-09 University Of Rochester Arenavirus vaccine
CN108977457B (en) * 2018-08-31 2021-04-02 长江大学 Preparation method of finless eel antibacterial peptide
CA3163139A1 (en) * 2018-12-27 2020-07-02 Vivienne I. Rebel Compositions and methods for treating cancer
CN110157710B (en) * 2019-07-11 2022-12-27 贵州大学 NaD1 gene promoter of nicotiana tabacum and application thereof
CN110343703B (en) * 2019-07-17 2022-12-27 中国科学院海洋研究所 Portunus trituberculatus C-type lectin PtCLec1 gene, and coding protein and application thereof
CN111019943A (en) * 2019-12-03 2020-04-17 西北农林科技大学 Promoter sequence of apple fructokinase gene, deletion mutant and application
CN112662670B (en) * 2020-12-25 2024-04-02 河南省农业科学院 Peanut fatty acyl-acyl carrier protein thioesterase AhFATB2 gene promoter, and preparation method and application thereof
WO2022175356A1 (en) * 2021-02-17 2022-08-25 Viralga Sas Control of green algae blooms
WO2023201300A2 (en) * 2022-04-14 2023-10-19 Shift Pharmaceuticals Holding Inc. Polynucleotide treatments for charcot-marie-tooth disease

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10236406C1 (en) * 2002-08-02 2003-12-24 Epigenomics Ag Method for the amplification of nucleic acids of low complexity
KR101293713B1 (en) * 2004-12-03 2013-08-12 휴먼 제네틱 시그너처스 피티와이 엘티디 Methods for simplifying microbial nucleic acids by chemical modification of cytosines

Also Published As

Publication number Publication date
JP2009538603A (en) 2009-11-12
AU2006344331A1 (en) 2007-12-13
CN101522908A (en) 2009-09-02
EP2021497A1 (en) 2009-02-11
CA2654010A1 (en) 2007-12-13
WO2007140506A1 (en) 2007-12-13

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