CO6241166A2 - Metodos de produccion de proteinas utilizando compuestos anti-senectud - Google Patents
Metodos de produccion de proteinas utilizando compuestos anti-senectudInfo
- Publication number
- CO6241166A2 CO6241166A2 CO09118627A CO09118627A CO6241166A2 CO 6241166 A2 CO6241166 A2 CO 6241166A2 CO 09118627 A CO09118627 A CO 09118627A CO 09118627 A CO09118627 A CO 09118627A CO 6241166 A2 CO6241166 A2 CO 6241166A2
- Authority
- CO
- Colombia
- Prior art keywords
- cell culture
- senescence compound
- carnosine
- increased
- cell
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70578—NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/32—Amino acids
- C12N2500/33—Amino acids other than alpha-amino carboxylic acids, e.g. beta-amino acids, taurine
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Biomedical Technology (AREA)
- Toxicology (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Se describen los métodos para producir una proteína en cultivo celular que comprende un compuesto anti-senectud, tal como el antioxidante carnosina. De acuerdo a las enseñanzas de la presente invención, las células desarrolladas en un medio de cultivo celular que comprende un compuesto anti-senectud muestran viabilidad y productividad incrementadas. Además, los cultivos celulares desarrollados en presencia de un compuesto anti-senectud muestran niveles disminuidos de agregados de alto peso molecular en el medio de cultivo celular. 1.- Un método para producir una proteína de fusión TNFR en cultivo celular, caracterizado porque comprende los pasos de: cultivar las células de mamífero que contienen un gen que codifica para la proteína de fusión TNFR, cuyo gen es expresado bajo condiciones de cultivo celular, en un medio de cultivo celular que comprende un compuesto anti-senectud; y mantener el cultivo bajo condiciones y por un tiempo suficiente para permitir la expresión de la proteína;en donde el cultivo celular muestra una característica mejorada de cultivo celular que difiere de una característica correspondiente de cultivo celular que pudiera ser observada bajo condiciones de otro modo idénticas en un medio de otro nodo idéntico, que carece del compuesto anti-senectud; en donde la característica de cultivo mejorada se selecciona del grupo que consiste de: título incrementado, productividad específica celular incrementada, viabilidad celular incrementada, densidad incrementada de células viables integradas, acumulación disminuida de agregados de alto peso molecular, acumulación disminuida de especies ácidas, y combinaciones de las mismas. 2.- El método de conformidad con la reivindicación 1, caracterizado porque el compuesto anti-senectud se selecciona del grupo que consiste de carnosina, acetil-carnosina, homocarnosina, anserina, y beta-alanina y combinaciones de las mismas. 3.- El método de conformidad con la reivindicación 1, caracterizado porque el compuesto anti-senectud comprende carnosina. 4.- El método de conformidad con la reivindicación 1, 2 ó 3, caracterizado porque el compuesto anti-senectud está presente en el medio de cultivo celular a una concentración de entre aproximadamente 5 mM y aproximadamente100 mM.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US91338207P | 2007-04-23 | 2007-04-23 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CO6241166A2 true CO6241166A2 (es) | 2011-01-20 |
Family
ID=39493848
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CO09117978A CO6241165A2 (es) | 2007-04-23 | 2009-10-21 | Uso de temperatura baja y/o ph bajo en el cultivo de celulas |
| CO09118627A CO6241166A2 (es) | 2007-04-23 | 2009-10-22 | Metodos de produccion de proteinas utilizando compuestos anti-senectud |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CO09117978A CO6241165A2 (es) | 2007-04-23 | 2009-10-21 | Uso de temperatura baja y/o ph bajo en el cultivo de celulas |
Country Status (26)
| Country | Link |
|---|---|
| US (2) | US9988662B2 (es) |
| EP (2) | EP2139986B1 (es) |
| JP (3) | JP5557736B2 (es) |
| KR (3) | KR101560421B1 (es) |
| CN (3) | CN101668845B (es) |
| AR (2) | AR066238A1 (es) |
| AU (2) | AU2008242633B2 (es) |
| BR (2) | BRPI0810511A2 (es) |
| CA (2) | CA2684727C (es) |
| CL (2) | CL2008001159A1 (es) |
| CO (2) | CO6241165A2 (es) |
| DK (2) | DK2139986T3 (es) |
| ES (2) | ES2424217T3 (es) |
| HK (2) | HK1142095A1 (es) |
| HU (1) | HUE034776T2 (es) |
| IL (2) | IL201720A (es) |
| MX (2) | MX2009011363A (es) |
| PA (2) | PA8777701A1 (es) |
| PE (2) | PE20090731A1 (es) |
| PL (2) | PL2139987T3 (es) |
| PT (2) | PT2139987E (es) |
| RU (2) | RU2478702C2 (es) |
| SI (2) | SI2139987T1 (es) |
| TW (2) | TW200902708A (es) |
| WO (2) | WO2008131374A1 (es) |
| ZA (1) | ZA200907426B (es) |
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| EP2188371B1 (en) | 2007-08-09 | 2017-12-20 | Wyeth LLC | Use of perfusion to enhance production of fed-batch cell culture in bioreactors |
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