DE602005027414D1 - Verfahren zur übertragung episomaler vektoren in tierzellen - Google Patents

Verfahren zur übertragung episomaler vektoren in tierzellen

Info

Publication number
DE602005027414D1
DE602005027414D1 DE602005027414T DE602005027414T DE602005027414D1 DE 602005027414 D1 DE602005027414 D1 DE 602005027414D1 DE 602005027414 T DE602005027414 T DE 602005027414T DE 602005027414 T DE602005027414 T DE 602005027414T DE 602005027414 D1 DE602005027414 D1 DE 602005027414D1
Authority
DE
Germany
Prior art keywords
transmitting
episomal vectors
animal cells
transfer
episomal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602005027414T
Other languages
English (en)
Inventor
Marialuisa Lavitrano
Maria Laura Bacci
Monica Forni
Hans Joachim Lipps
Pierluigi Donini
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universita degli Studi di Milano Bicocca
Original Assignee
Universita degli Studi di Milano Bicocca
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universita degli Studi di Milano Bicocca filed Critical Universita degli Studi di Milano Bicocca
Publication of DE602005027414D1 publication Critical patent/DE602005027414D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8778—Swine embryos
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/10—Conditioning of cells for in vitro fecondation or nuclear transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE602005027414T 2005-10-05 2005-10-05 Verfahren zur übertragung episomaler vektoren in tierzellen Active DE602005027414D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IT2005/000573 WO2007039920A1 (en) 2005-10-05 2005-10-05 A method for the transfer of episomal vectors into animal cells

Publications (1)

Publication Number Publication Date
DE602005027414D1 true DE602005027414D1 (de) 2011-05-19

Family

ID=35645877

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602005027414T Active DE602005027414D1 (de) 2005-10-05 2005-10-05 Verfahren zur übertragung episomaler vektoren in tierzellen

Country Status (7)

Country Link
US (1) US9068200B2 (de)
EP (1) EP1943345B1 (de)
JP (1) JP2009511010A (de)
AT (1) ATE504655T1 (de)
DE (1) DE602005027414D1 (de)
ES (1) ES2364342T3 (de)
WO (1) WO2007039920A1 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102007022274A1 (de) * 2007-05-09 2008-11-20 Jacobs University Bremen Ggmbh Verfahren und Reagenzien zur Untersuchung von Nukleinsäure-Methylierungsreaktionen
US20150024497A1 (en) * 2013-07-19 2015-01-22 Elwha Llc Methods and Systems for Utilizing Sperm for Molecular Delivery
CN103642838B (zh) * 2013-11-29 2015-08-26 新乡医学院 一种人类及哺乳动物细胞表达载体及其应用
EP3456821B2 (de) * 2017-09-19 2024-01-24 Deutsches Krebsforschungszentrum Nicht integrierende dna-vektoren zur genetischen modifikation von zellen

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19848017A1 (de) * 1998-10-17 2000-04-20 Multigene Biotech Gmbh Episomal replizierender Vektor zur Expression eines Transgens in Säugerzellen
US20020086428A1 (en) * 2000-09-01 2002-07-04 The Regents Of The University Of California, Office Of Technology Transfer Methods and compositions for independent DNA replication in eukaryotic cells
WO2002034040A2 (en) * 2000-10-23 2002-05-02 Mermaid Pharmaceuticals Gmbh Method for generating transgenic fish embryos using an episomal vector system
PT1354034E (pt) * 2000-11-30 2008-02-28 Medarex Inc Roedores transgénicos transcromossómicos para produção de anticorpos humanos
DE10109780A1 (de) * 2001-02-28 2002-09-05 Cardion Ag Episomale Vektoren enthaltend Matrix-Anheftungsregionen zur zellspezifischen Genexpression

Also Published As

Publication number Publication date
US9068200B2 (en) 2015-06-30
ATE504655T1 (de) 2011-04-15
EP1943345B1 (de) 2011-04-06
JP2009511010A (ja) 2009-03-19
US20090158450A1 (en) 2009-06-18
ES2364342T3 (es) 2011-08-31
WO2007039920A1 (en) 2007-04-12
EP1943345A1 (de) 2008-07-16

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