CN1463364A - 免疫反应测定方法及其所使用的免疫反应测定试剂 - Google Patents
免疫反应测定方法及其所使用的免疫反应测定试剂 Download PDFInfo
- Publication number
- CN1463364A CN1463364A CN02802082A CN02802082A CN1463364A CN 1463364 A CN1463364 A CN 1463364A CN 02802082 A CN02802082 A CN 02802082A CN 02802082 A CN02802082 A CN 02802082A CN 1463364 A CN1463364 A CN 1463364A
- Authority
- CN
- China
- Prior art keywords
- antigen
- antibody
- immunoreaction measurement
- reagent
- tricarboxylate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 91
- 238000000034 method Methods 0.000 title claims abstract description 53
- 230000036046 immunoreaction Effects 0.000 title claims description 119
- 238000003556 assay Methods 0.000 title claims description 13
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 149
- 238000013016 damping Methods 0.000 claims description 129
- 239000012530 fluid Substances 0.000 claims description 129
- 238000006243 chemical reaction Methods 0.000 claims description 127
- 239000000427 antigen Substances 0.000 claims description 119
- 102000036639 antigens Human genes 0.000 claims description 119
- 108091007433 antigens Proteins 0.000 claims description 119
- 150000003628 tricarboxylic acids Chemical class 0.000 claims description 95
- 238000005259 measurement Methods 0.000 claims description 80
- 238000000691 measurement method Methods 0.000 claims description 60
- GTZCVFVGUGFEME-UHFFFAOYSA-N trans-aconitic acid Natural products OC(=O)CC(C(O)=O)=CC(O)=O GTZCVFVGUGFEME-UHFFFAOYSA-N 0.000 claims description 56
- 102000008100 Human Serum Albumin Human genes 0.000 claims description 50
- 108091006905 Human Serum Albumin Proteins 0.000 claims description 50
- 239000000203 mixture Substances 0.000 claims description 45
- 239000000463 material Substances 0.000 claims description 43
- 150000001455 metallic ions Chemical class 0.000 claims description 16
- 239000002253 acid Substances 0.000 claims description 13
- 150000001875 compounds Chemical class 0.000 claims description 13
- 230000008859 change Effects 0.000 claims description 11
- 239000001124 (E)-prop-1-ene-1,2,3-tricarboxylic acid Substances 0.000 claims description 10
- 229940091181 aconitic acid Drugs 0.000 claims description 10
- 239000010695 polyglycol Substances 0.000 claims description 8
- 229920000151 polyglycol Polymers 0.000 claims description 8
- 230000001900 immune effect Effects 0.000 claims description 6
- 230000003287 optical effect Effects 0.000 claims description 5
- 229910021645 metal ion Inorganic materials 0.000 claims description 3
- GTZCVFVGUGFEME-IWQZZHSRSA-N cis-aconitic acid Chemical compound OC(=O)C\C(C(O)=O)=C\C(O)=O GTZCVFVGUGFEME-IWQZZHSRSA-N 0.000 claims 2
- 239000000126 substance Substances 0.000 abstract description 10
- 150000003839 salts Chemical class 0.000 abstract description 6
- 230000002378 acidificating effect Effects 0.000 abstract description 3
- 150000003627 tricarboxylic acid derivatives Chemical class 0.000 abstract 2
- 239000000243 solution Substances 0.000 description 146
- 102100032752 C-reactive protein Human genes 0.000 description 61
- GTZCVFVGUGFEME-HNQUOIGGSA-N trans-aconitic acid Chemical compound OC(=O)C\C(C(O)=O)=C/C(O)=O GTZCVFVGUGFEME-HNQUOIGGSA-N 0.000 description 51
- 229960004106 citric acid Drugs 0.000 description 45
- 230000000694 effects Effects 0.000 description 31
- DVLFYONBTKHTER-UHFFFAOYSA-N 3-(N-morpholino)propanesulfonic acid Chemical compound OS(=O)(=O)CCCN1CCOCC1 DVLFYONBTKHTER-UHFFFAOYSA-N 0.000 description 22
- 238000002360 preparation method Methods 0.000 description 22
- 239000007993 MOPS buffer Substances 0.000 description 21
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 20
- 229960003511 macrogol Drugs 0.000 description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 20
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 18
- 230000000052 comparative effect Effects 0.000 description 16
- 102000009027 Albumins Human genes 0.000 description 15
- 108010088751 Albumins Proteins 0.000 description 15
- 239000007979 citrate buffer Substances 0.000 description 13
- 230000009467 reduction Effects 0.000 description 13
- KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 11
- 238000005303 weighing Methods 0.000 description 11
- 239000000725 suspension Substances 0.000 description 10
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 9
- 210000004408 hybridoma Anatomy 0.000 description 9
- 229910001424 calcium ion Inorganic materials 0.000 description 8
- 150000004677 hydrates Chemical class 0.000 description 8
- 238000004848 nephelometry Methods 0.000 description 8
- 239000007787 solid Substances 0.000 description 8
- 241000699666 Mus <mouse, genus> Species 0.000 description 7
- 239000000872 buffer Substances 0.000 description 7
- 238000000502 dialysis Methods 0.000 description 7
- 238000010790 dilution Methods 0.000 description 7
- 239000012895 dilution Substances 0.000 description 7
- 238000002156 mixing Methods 0.000 description 7
- 230000007935 neutral effect Effects 0.000 description 7
- 229920003169 water-soluble polymer Polymers 0.000 description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 101710153593 Albumin A Proteins 0.000 description 5
- 206010003445 Ascites Diseases 0.000 description 5
- 230000004520 agglutination Effects 0.000 description 5
- 239000007864 aqueous solution Substances 0.000 description 5
- 239000007853 buffer solution Substances 0.000 description 5
- 239000011575 calcium Substances 0.000 description 5
- 238000004440 column chromatography Methods 0.000 description 5
- 230000001419 dependent effect Effects 0.000 description 5
- 230000028993 immune response Effects 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 239000001509 sodium citrate Substances 0.000 description 5
- 239000001384 succinic acid Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 5
- 229940038773 trisodium citrate Drugs 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 4
- 241001482592 Oreamnos americanus Species 0.000 description 4
- 229960004543 anhydrous citric acid Drugs 0.000 description 4
- 230000000890 antigenic effect Effects 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- FWBOFUGDKHMVPI-UHFFFAOYSA-K dicopper;2-oxidopropane-1,2,3-tricarboxylate Chemical compound [Cu+2].[Cu+2].[O-]C(=O)CC([O-])(C([O-])=O)CC([O-])=O FWBOFUGDKHMVPI-UHFFFAOYSA-K 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 239000006193 liquid solution Substances 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- ODBLHEXUDAPZAU-UHFFFAOYSA-N threo-D-isocitric acid Natural products OC(=O)C(O)C(C(O)=O)CC(O)=O ODBLHEXUDAPZAU-UHFFFAOYSA-N 0.000 description 4
- 241000283707 Capra Species 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 3
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 3
- 101000942118 Homo sapiens C-reactive protein Proteins 0.000 description 3
- 101000922020 Homo sapiens Cysteine and glycine-rich protein 1 Proteins 0.000 description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- 239000012980 RPMI-1640 medium Substances 0.000 description 3
- 235000011941 Tilia x europaea Nutrition 0.000 description 3
- 210000000683 abdominal cavity Anatomy 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 229940091179 aconitate Drugs 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 239000012149 elution buffer Substances 0.000 description 3
- 239000004571 lime Substances 0.000 description 3
- 239000011777 magnesium Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 239000004094 surface-active agent Substances 0.000 description 3
- ODBLHEXUDAPZAU-ZAFYKAAXSA-N D-threo-isocitric acid Chemical compound OC(=O)[C@H](O)[C@@H](C(O)=O)CC(O)=O ODBLHEXUDAPZAU-ZAFYKAAXSA-N 0.000 description 2
- 208000007342 Diabetic Nephropathies Diseases 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
- ODBLHEXUDAPZAU-FONMRSAGSA-N Isocitric acid Natural products OC(=O)[C@@H](O)[C@H](C(O)=O)CC(O)=O ODBLHEXUDAPZAU-FONMRSAGSA-N 0.000 description 2
- WMFOQBRAJBCJND-UHFFFAOYSA-M Lithium hydroxide Chemical compound [Li+].[OH-] WMFOQBRAJBCJND-UHFFFAOYSA-M 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- 101710120037 Toxin CcdB Proteins 0.000 description 2
- GPSAAQWVJOVCBK-UHFFFAOYSA-N [K].[K].[K].OC(=O)CC(O)(C(O)=O)CC(O)=O Chemical compound [K].[K].[K].OC(=O)CC(O)(C(O)=O)CC(O)=O GPSAAQWVJOVCBK-UHFFFAOYSA-N 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000003139 buffering effect Effects 0.000 description 2
- FNAQSUUGMSOBHW-UHFFFAOYSA-H calcium citrate Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O FNAQSUUGMSOBHW-UHFFFAOYSA-H 0.000 description 2
- 239000001354 calcium citrate Substances 0.000 description 2
- KLOIYEQEVSIOOO-UHFFFAOYSA-N carbocromen Chemical compound CC1=C(CCN(CC)CC)C(=O)OC2=CC(OCC(=O)OCC)=CC=C21 KLOIYEQEVSIOOO-UHFFFAOYSA-N 0.000 description 2
- 208000033679 diabetic kidney disease Diseases 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 2
- 229910000397 disodium phosphate Inorganic materials 0.000 description 2
- 235000019800 disodium phosphate Nutrition 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 235000013905 glycine and its sodium salt Nutrition 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 102000051143 human CRP Human genes 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 239000001508 potassium citrate Substances 0.000 description 2
- 229960002635 potassium citrate Drugs 0.000 description 2
- QEEAPRPFLLJWCF-UHFFFAOYSA-K potassium citrate (anhydrous) Chemical compound [K+].[K+].[K+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O QEEAPRPFLLJWCF-UHFFFAOYSA-K 0.000 description 2
- 235000011082 potassium citrates Nutrition 0.000 description 2
- XOJVVFBFDXDTEG-UHFFFAOYSA-N pristane Chemical compound CC(C)CCCC(C)CCCC(C)CCCC(C)C XOJVVFBFDXDTEG-UHFFFAOYSA-N 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 description 2
- 239000001393 triammonium citrate Substances 0.000 description 2
- 235000011046 triammonium citrate Nutrition 0.000 description 2
- 235000013337 tricalcium citrate Nutrition 0.000 description 2
- SOBHUZYZLFQYFK-UHFFFAOYSA-K trisodium;hydroxy-[[phosphonatomethyl(phosphonomethyl)amino]methyl]phosphinate Chemical compound [Na+].[Na+].[Na+].OP(O)(=O)CN(CP(O)([O-])=O)CP([O-])([O-])=O SOBHUZYZLFQYFK-UHFFFAOYSA-K 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- UMCMPZBLKLEWAF-BCTGSCMUSA-N 3-[(3-cholamidopropyl)dimethylammonio]propane-1-sulfonate Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)C)[C@@]2(C)[C@@H](O)C1 UMCMPZBLKLEWAF-BCTGSCMUSA-N 0.000 description 1
- 238000011725 BALB/c mouse Methods 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 108010074051 C-Reactive Protein Proteins 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 102000009151 Luteinizing Hormone Human genes 0.000 description 1
- 108010073521 Luteinizing Hormone Proteins 0.000 description 1
- 206010029719 Nonspecific reaction Diseases 0.000 description 1
- 206010035226 Plasma cell myeloma Diseases 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- BZHJMEDXRYGGRV-UHFFFAOYSA-N Vinyl chloride Chemical compound ClC=C BZHJMEDXRYGGRV-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- KGUHOFWIXKIURA-VQXBOQCVSA-N [(2r,3s,4s,5r,6r)-6-[(2s,3s,4s,5r)-3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]oxy-3,4,5-trihydroxyoxan-2-yl]methyl dodecanoate Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](COC(=O)CCCCCCCCCCC)O[C@@H]1O[C@@]1(CO)[C@@H](O)[C@H](O)[C@@H](CO)O1 KGUHOFWIXKIURA-VQXBOQCVSA-N 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 238000011091 antibody purification Methods 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 230000000621 autoagglutination Effects 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 1
- 230000007910 cell fusion Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000009920 chelation Effects 0.000 description 1
- 229960004407 chorionic gonadotrophin Drugs 0.000 description 1
- 229940001468 citrate Drugs 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- CSVGEMRSDNSWRF-UHFFFAOYSA-L disodium;dihydrogen phosphate Chemical compound [Na+].[Na+].OP(O)([O-])=O.OP(O)([O-])=O CSVGEMRSDNSWRF-UHFFFAOYSA-L 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 238000013399 early diagnosis Methods 0.000 description 1
- CCIVGXIOQKPBKL-UHFFFAOYSA-N ethanesulfonic acid Chemical compound CCS(O)(=O)=O CCIVGXIOQKPBKL-UHFFFAOYSA-N 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 239000012847 fine chemical Substances 0.000 description 1
- 238000012921 fluorescence analysis Methods 0.000 description 1
- 230000003325 follicular Effects 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000007499 fusion processing Methods 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 238000010324 immunological assay Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000007912 intraperitoneal administration Methods 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 239000006249 magnetic particle Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 229910000403 monosodium phosphate Inorganic materials 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- 201000000050 myeloid neoplasm Diseases 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- HEGSGKPQLMEBJL-RKQHYHRCSA-N octyl beta-D-glucopyranoside Chemical compound CCCCCCCCO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HEGSGKPQLMEBJL-RKQHYHRCSA-N 0.000 description 1
- 239000005304 optical glass Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000003352 sequestering agent Substances 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- IHQKEDIOMGYHEB-UHFFFAOYSA-M sodium dimethylarsinate Chemical compound [Na+].C[As](C)([O-])=O IHQKEDIOMGYHEB-UHFFFAOYSA-M 0.000 description 1
- DAJSVUQLFFJUSX-UHFFFAOYSA-M sodium;dodecane-1-sulfonate Chemical compound [Na+].CCCCCCCCCCCCS([O-])(=O)=O DAJSVUQLFFJUSX-UHFFFAOYSA-M 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 229940032085 sucrose monolaurate Drugs 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
Claims (23)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP179710/2001 | 2001-06-14 | ||
JP2001179710 | 2001-06-14 | ||
JP368286/2001 | 2001-12-03 | ||
JP2001368286A JP2003066047A (ja) | 2001-06-14 | 2001-12-03 | 免疫反応測定方法及びそれに用いる免疫反応測定用試薬 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1463364A true CN1463364A (zh) | 2003-12-24 |
CN1258089C CN1258089C (zh) | 2006-05-31 |
Family
ID=26616877
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB028020820A Expired - Lifetime CN1258089C (zh) | 2001-06-14 | 2002-06-05 | 免疫反应测定方法及其所使用的免疫反应测定试剂 |
Country Status (7)
Country | Link |
---|---|
US (1) | US20030180806A1 (zh) |
EP (1) | EP1396724B1 (zh) |
JP (1) | JP2003066047A (zh) |
KR (1) | KR100549360B1 (zh) |
CN (1) | CN1258089C (zh) |
DE (1) | DE60213984T2 (zh) |
WO (1) | WO2003010541A1 (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109564223A (zh) * | 2016-09-06 | 2019-04-02 | 富士瑞必欧株式会社 | 肿瘤标记物的测定方法及测定试剂 |
CN110618263A (zh) * | 2018-06-20 | 2019-12-27 | 厦门万泰凯瑞生物技术有限公司 | 一种c反应蛋白全程检测的方法以及相应的试剂盒 |
CN114544978A (zh) * | 2022-03-04 | 2022-05-27 | 美康生物科技股份有限公司 | 一种igf-1检测试剂盒及其制备方法和检测方法 |
Families Citing this family (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1679516A1 (en) * | 2003-09-26 | 2006-07-12 | Matsushita Electric Industrial Co., Ltd. | Method of immunoreaction measurement and, for use therein, reagent, kit and optical cell |
JPWO2006057225A1 (ja) * | 2004-11-25 | 2008-06-05 | 松下電器産業株式会社 | センサデバイス |
DE602006013053D1 (de) * | 2006-12-15 | 2010-04-29 | Biotrin Intellectual Pty Ltd | Verfahren zum nachweis von menschlichem parvovirus-antigen |
NZ597767A (en) | 2007-05-21 | 2013-06-28 | Bristol Myers Squibb Co | Antibodies to IL-6 and use thereof |
US8178101B2 (en) | 2007-05-21 | 2012-05-15 | Alderbio Holdings Inc. | Use of anti-IL-6 antibodies having specific binding properties to treat cachexia |
US8404235B2 (en) | 2007-05-21 | 2013-03-26 | Alderbio Holdings Llc | Antagonists of IL-6 to raise albumin and/or lower CRP |
US8252286B2 (en) | 2007-05-21 | 2012-08-28 | Alderbio Holdings Llc | Antagonists of IL-6 to prevent or treat thrombosis |
US8062864B2 (en) | 2007-05-21 | 2011-11-22 | Alderbio Holdings Llc | Nucleic acids encoding antibodies to IL-6, and recombinant production of anti-IL-6 antibodies |
US7906117B2 (en) | 2007-05-21 | 2011-03-15 | Alderbio Holdings Llc | Antagonists of IL-6 to prevent or treat cachexia, weakness, fatigue, and/or fever |
US9701747B2 (en) | 2007-05-21 | 2017-07-11 | Alderbio Holdings Llc | Method of improving patient survivability and quality of life by anti-IL-6 antibody administration |
US8323649B2 (en) | 2008-11-25 | 2012-12-04 | Alderbio Holdings Llc | Antibodies to IL-6 and use thereof |
EP2361096B1 (en) * | 2008-11-25 | 2019-01-02 | AlderBio Holdings LLC | Il-6-specific antibody to prevent or treat cachexia |
US9212223B2 (en) | 2008-11-25 | 2015-12-15 | Alderbio Holdings Llc | Antagonists of IL-6 to prevent or treat thrombosis |
US9452227B2 (en) | 2008-11-25 | 2016-09-27 | Alderbio Holdings Llc | Methods of treating or diagnosing conditions associated with elevated IL-6 using anti-IL-6 antibodies or fragments |
US8420089B2 (en) | 2008-11-25 | 2013-04-16 | Alderbio Holdings Llc | Antagonists of IL-6 to raise albumin and/or lower CRP |
US8992920B2 (en) | 2008-11-25 | 2015-03-31 | Alderbio Holdings Llc | Anti-IL-6 antibodies for the treatment of arthritis |
US8337847B2 (en) | 2008-11-25 | 2012-12-25 | Alderbio Holdings Llc | Methods of treating anemia using anti-IL-6 antibodies |
US9775921B2 (en) | 2009-11-24 | 2017-10-03 | Alderbio Holdings Llc | Subcutaneously administrable composition containing anti-IL-6 antibody |
US9468676B2 (en) | 2009-11-24 | 2016-10-18 | Alderbio Holdings Llc | Antagonists of IL-6 to prevent or treat thrombosis |
WO2012071554A2 (en) | 2010-11-23 | 2012-05-31 | Alder Biopharmaceuticals, Inc. | Anti-il-6 antibodies for the treatment of oral mucositis |
WO2013108168A2 (en) * | 2012-01-16 | 2013-07-25 | Koninklijke Philips N.V. | Determining a presence of target molecules in a body fluid comprising cells |
JP6736797B1 (ja) | 2018-11-09 | 2020-08-05 | 積水メディカル株式会社 | 自動分析装置での免疫測定における異常検出抑制方法、及び免疫測定試薬 |
CN114152755A (zh) * | 2021-12-06 | 2022-03-08 | 石家庄斯巴克生物科技有限公司 | 一种c反应蛋白检测试剂处理液、试剂盒及检测方法 |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS57182169A (en) * | 1981-05-02 | 1982-11-09 | Mitsubishi Chem Ind Ltd | Measuring method for antigen-antibody reaction |
JPS6057257A (ja) * | 1983-09-09 | 1985-04-03 | Hitachi Ltd | イムノアツセイ法 |
US4931385A (en) * | 1985-06-24 | 1990-06-05 | Hygeia Sciences, Incorporated | Enzyme immunoassays and immunologic reagents |
GB8800702D0 (en) * | 1988-01-13 | 1988-02-10 | Nycomed As | Test method & reagent kit therefor |
JP2572267B2 (ja) * | 1988-07-27 | 1997-01-16 | 雪印乳業株式会社 | 紛末状非特異的吸着防止剤の製造方法 |
JP2691575B2 (ja) * | 1988-08-26 | 1997-12-17 | 第一化学薬品株式会社 | 免疫反応の測定方法 |
US5272258A (en) * | 1989-06-29 | 1993-12-21 | Rush-Presbyterian-St. Luke's Medical Center | Monoclonal antibodies to C-reactive protein |
WO1991010911A1 (en) * | 1990-01-19 | 1991-07-25 | Repligen Corporation | Immunoassay of protein a under acidic conditions |
JPH0682450A (ja) * | 1992-09-04 | 1994-03-22 | Eiken Chem Co Ltd | 免疫学的測定試薬 |
US5358852A (en) * | 1992-12-21 | 1994-10-25 | Eastman Kodak Company | Use of calcium in immunoassay for measurement of C-reactive protein |
JP3337818B2 (ja) * | 1994-03-31 | 2002-10-28 | 雪印乳業株式会社 | 非特異的吸着防止剤 |
US5616460A (en) * | 1995-06-07 | 1997-04-01 | Abbott Laboratories | Buffer composition for reagents for immunoassay |
JP3886639B2 (ja) * | 1998-06-01 | 2007-02-28 | 栄研化学株式会社 | 免疫学的凝集反応試薬およびこれを用いたプロゾーン現象の抑制方法 |
JP4219491B2 (ja) * | 1999-06-18 | 2009-02-04 | 富士フイルム株式会社 | 乾式分析方法及び乾式分析要素 |
-
2001
- 2001-12-03 JP JP2001368286A patent/JP2003066047A/ja not_active Withdrawn
-
2002
- 2002-06-05 US US10/311,775 patent/US20030180806A1/en not_active Abandoned
- 2002-06-05 DE DE60213984T patent/DE60213984T2/de not_active Expired - Lifetime
- 2002-06-05 EP EP02733326A patent/EP1396724B1/en not_active Expired - Lifetime
- 2002-06-05 WO PCT/JP2002/005568 patent/WO2003010541A1/ja active IP Right Grant
- 2002-06-05 KR KR1020037002112A patent/KR100549360B1/ko not_active IP Right Cessation
- 2002-06-05 CN CNB028020820A patent/CN1258089C/zh not_active Expired - Lifetime
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109564223A (zh) * | 2016-09-06 | 2019-04-02 | 富士瑞必欧株式会社 | 肿瘤标记物的测定方法及测定试剂 |
CN110618263A (zh) * | 2018-06-20 | 2019-12-27 | 厦门万泰凯瑞生物技术有限公司 | 一种c反应蛋白全程检测的方法以及相应的试剂盒 |
CN110618263B (zh) * | 2018-06-20 | 2021-10-29 | 厦门万泰凯瑞生物技术有限公司 | 一种c反应蛋白全程检测的方法以及相应的试剂盒 |
CN114544978A (zh) * | 2022-03-04 | 2022-05-27 | 美康生物科技股份有限公司 | 一种igf-1检测试剂盒及其制备方法和检测方法 |
Also Published As
Publication number | Publication date |
---|---|
KR20030031153A (ko) | 2003-04-18 |
EP1396724B1 (en) | 2006-08-16 |
KR100549360B1 (ko) | 2006-02-08 |
WO2003010541A1 (fr) | 2003-02-06 |
US20030180806A1 (en) | 2003-09-25 |
CN1258089C (zh) | 2006-05-31 |
DE60213984D1 (de) | 2006-09-28 |
EP1396724A1 (en) | 2004-03-10 |
DE60213984T2 (de) | 2007-01-25 |
EP1396724A4 (en) | 2004-07-14 |
JP2003066047A (ja) | 2003-03-05 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN1258089C (zh) | 免疫反应测定方法及其所使用的免疫反应测定试剂 | |
CA2964771C (en) | Anti-human igm monoclonal antibody and immunoassay using the same | |
WO2010058860A1 (ja) | 試料中のc反応性蛋白質の測定方法及び測定試薬 | |
JP2011530514A5 (zh) | ||
KR20200024304A (ko) | 항인간 IgG4 모노클로날 항체, 및 그 항체를 이용한 인간 IgG4 측정 시약 | |
CN1247996C (zh) | 免疫反应测定方法以及方法中使用的免疫反应测定用试剂盒 | |
JPS60237363A (ja) | 免疫グロブリンの定量方法 | |
CN1314965C (zh) | 免疫学的测定方法 | |
EP2776464A1 (en) | Antibody specific for trans - resveratrol and use thereof | |
JP7315968B2 (ja) | 生物学的試料中の遊離aimの免疫学的分析方法及び対象におけるnashの検出方法 | |
CN110133270A (zh) | 一种增强免疫抑制比浊抗体筛选试剂及其制作和使用方法 | |
JPH1090268A (ja) | 免疫学的粒子凝集反応方法 | |
US20170241994A1 (en) | Composition for enzyme immunoassay using immunofluorescence and uses thereof | |
JP4452324B2 (ja) | 精製血清アルブミン及び免疫学的測定方法 | |
JP4689521B2 (ja) | 抗鉛モノクローナル抗体 | |
JP7315967B2 (ja) | 生物学的試料中の遊離aimの免疫学的分析方法及び測定キット | |
JP2515533B2 (ja) | 蛋白の定量方法 | |
JP7315966B2 (ja) | 生物学的試料中の遊離aimの免疫学的分析方法 | |
JPS62239056A (ja) | トランスフエリンの定量方法 | |
WO2024048583A1 (ja) | 免疫学的測定方法、非特異反応抑制方法、免疫学的測定試薬、免疫学的測定試薬キット、組成物、非特異反応抑制剤、及び使用 | |
TW201003071A (en) | Test specimen and method for detecting inflammation, polyclonal and monoclonal antibodies made by the same, hybridoma cell lines for producing the antibodies and applications of the antibodies | |
JP2010271270A (ja) | ヒト心筋組織由来の脂肪酸結合蛋白の測定試薬 | |
JPS62238461A (ja) | 補体の定量方法 | |
CN1692281A (zh) | 免疫反应测定方法 | |
JPH07103979A (ja) | 競合法を利用する生体成分の測定方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
ASS | Succession or assignment of patent right |
Owner name: PANASONIC HEALTHCARE + MEDICAL EQUIPMENT CO., LTD. Free format text: FORMER OWNER: MATSUSHITA ELECTRIC INDUSTRIAL CO, LTD. Effective date: 20140428 |
|
C41 | Transfer of patent application or patent right or utility model | ||
TR01 | Transfer of patent right |
Effective date of registration: 20140428 Address after: Ehime Prefecture, Japan Patentee after: Panasonic Healthcare Co., Ltd Address before: Osaka Patentee before: Matsushita Electric Industrial Co., Ltd. |
|
ASS | Succession or assignment of patent right |
Owner name: PANASONIC HEALTHCARE HOLDINGS CO., LTD. Free format text: FORMER OWNER: PANASONIC HEALTHCARE + MEDICAL EQUIPMENT CO., LTD. Effective date: 20150403 |
|
TR01 | Transfer of patent right |
Effective date of registration: 20150403 Address after: Tokyo, Japan Patentee after: Panasonic's health medical treatment is controlled interest Co., Ltd. Address before: Japan Ehime Patentee before: Panasonic Healthcare Co., Ltd |
|
CP01 | Change in the name or title of a patent holder | ||
CP01 | Change in the name or title of a patent holder |
Address after: Tokyo, Japan Patentee after: Pu Hei holding company Address before: Tokyo, Japan Patentee before: Panasonic's health medical treatment is controlled interest Co., Ltd. |
|
CX01 | Expiry of patent term | ||
CX01 | Expiry of patent term |
Granted publication date: 20060531 |