CN1303214C - 分离的荧光素酶及其用途 - Google Patents
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
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- C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
- C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
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Abstract
Description
基因产物 | 生物 | 底物 | λ | 表达 | 参考文献/专利 |
Lux基因 | Vibrio fischerii | FMN,十二烷醛NADH | 495nm | 细胞质 | Apley et al.,1985Gustafson G.,US 5196524 |
基因产物 | 生物 | 底物 | λ | 表达 | 参考文献/专利 |
Renilla荧光素酶 | Renillareniformis | Coelenterazine | 480nm | 细胞质 | Mathews et al.,1977Lorenz et al,1991Lorenz et al.1996Alan,P;WO 0020619Milton J.,US 5418155Roelant C.,WO 9938999 |
Vargula/Cypridia荧光素酶 | Vargulahilgendorferii | Vargula荧光素 | 460nm | 分泌 | Thomspon et al.,1989Thompson et al.,1990Tora,JP 05064583Tora,JP 08027200Renard et al.,WO 9520653 |
Watasemia荧光素酶 | Wataseniascintillans | Watasemia荧光素 | ? | 细胞质 | Inoue et al.,1976 |
Olophorris荧光素酶 | Olophorusgracilirostris | Coelenterazine | 454 | 分泌 | Inouye et al,2000 |
水母蛋白 | Aequoriaaequoria | Coelenterazine(Ca2+激活的) | 470nm | 细胞质 | Head et al.2000Shimomura et al.,2000Jones et al.,1999Kendall et al.,1998Inouye et al.,1985Shimomura et al.,1969Cormier et al.,US 579844lCormier et al.,US 5422266 |
Obelin | Obelia | Coelenterazine | 470nm | 细胞质 | Matveev et al.,l999Berestovskaya,1999 |
基因产物 | 生物 | 底物 | λ | 表达 | 参考文献 |
萤火虫荧光素酶 | Photinuspyralis | 萤火虫荧光素,ATP | 550nm | 细胞质 | Webster et al.,1980Gould et al.,1988Sala-Newby et al,1992Bonin et al.,1994SherfB.,US 5670356KIKK,JP 09187281 |
Claims (6)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10058091.2 | 2000-11-23 | ||
DE10058091A DE10058091A1 (de) | 2000-11-23 | 2000-11-23 | Isolierte Luziferasen Lu164, LuAL und Lu22, sowie deren Verwendung |
Related Child Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN200510118849XA Division CN1847399B (zh) | 2000-11-23 | 2001-11-22 | 分离的荧光素酶及其用途 |
CN2012102094057A Division CN102732537A (zh) | 2000-11-23 | 2001-11-22 | 分离的荧光素酶及其用途 |
CN2010105056541A Division CN101979586B (zh) | 2000-11-23 | 2001-11-22 | 分离的荧光素酶及其用途 |
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CNB018194338A Expired - Lifetime CN1303214C (zh) | 2000-11-23 | 2001-11-22 | 分离的荧光素酶及其用途 |
CN2010105056541A Expired - Lifetime CN101979586B (zh) | 2000-11-23 | 2001-11-22 | 分离的荧光素酶及其用途 |
CN200510118849XA Expired - Lifetime CN1847399B (zh) | 2000-11-23 | 2001-11-22 | 分离的荧光素酶及其用途 |
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CN2010105056541A Expired - Lifetime CN101979586B (zh) | 2000-11-23 | 2001-11-22 | 分离的荧光素酶及其用途 |
CN200510118849XA Expired - Lifetime CN1847399B (zh) | 2000-11-23 | 2001-11-22 | 分离的荧光素酶及其用途 |
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US (4) | US7297483B2 (zh) |
EP (1) | EP1339853B1 (zh) |
JP (2) | JP4179877B2 (zh) |
KR (1) | KR100841808B1 (zh) |
CN (4) | CN102732537A (zh) |
AT (1) | ATE335824T1 (zh) |
AU (3) | AU2002220697A1 (zh) |
BR (2) | BRPI0115534B1 (zh) |
CA (1) | CA2429451C (zh) |
DE (2) | DE10058091A1 (zh) |
DK (1) | DK1339853T3 (zh) |
ES (1) | ES2271111T3 (zh) |
HK (3) | HK1096423A1 (zh) |
IL (2) | IL155878A0 (zh) |
MX (1) | MXPA03004539A (zh) |
PL (1) | PL206385B1 (zh) |
PT (1) | PT1339853E (zh) |
WO (2) | WO2002042469A1 (zh) |
ZA (1) | ZA200303928B (zh) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
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DE10058091A1 (de) | 2000-11-23 | 2002-06-06 | Bayer Ag | Isolierte Luziferasen Lu164, LuAL und Lu22, sowie deren Verwendung |
DE102007005803A1 (de) | 2007-02-06 | 2008-08-07 | Bayer Healthcare Ag | Sekretierte Luziferase MLuc7 und deren Verwendung |
DE102007005804A1 (de) | 2007-02-06 | 2008-08-07 | Bayer Healthcare Ag | Sekretierte Luziferase Lu164M3 und deren Verwendung |
JP5605727B2 (ja) | 2010-04-06 | 2014-10-15 | Jnc株式会社 | セレンテラジン類縁体及びセレンテラミド類縁体 |
JP6147669B2 (ja) * | 2010-12-03 | 2017-06-14 | ジーン・ストリーム・ピーティワイ・リミテッド | 改良光放射分子 |
US8435777B1 (en) | 2012-01-20 | 2013-05-07 | Cayla | CPG-free gene for a new secreted reporter protein |
JP6132350B2 (ja) * | 2012-10-26 | 2017-05-24 | 国立研究開発法人産業技術総合研究所 | 人工生物発光酵素 |
US10533231B2 (en) | 2012-10-26 | 2020-01-14 | National Institute Of Advanced Industrial Science And Technology | Artificial bioluminescent enzyme |
BR112015013710B1 (pt) * | 2012-12-12 | 2022-08-09 | Promega Corporation | Métodos in vitro para detectar uma interação entre um agente bioativo e um alvo celular |
US10214766B2 (en) | 2013-10-18 | 2019-02-26 | National Institute Of Advanced Industrial Science And Technology | Luminescent substrate for use in artificial bioluminescent enzyme |
Citations (2)
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US5604123A (en) * | 1988-08-09 | 1997-02-18 | Toray Industries, Inc. | Luciferase, gene encoding the same and production process of the same |
WO1999049019A2 (en) * | 1998-03-27 | 1999-09-30 | Prolume, Ltd. | Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics |
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US5838313A (en) * | 1995-11-20 | 1998-11-17 | Siemens Corporate Research, Inc. | Multimedia-based reporting system with recording and playback of dynamic annotation |
US6853375B2 (en) * | 2000-05-10 | 2005-02-08 | Cognos Incorporated | Method for preemptive screen rendering |
DE10058091A1 (de) * | 2000-11-23 | 2002-06-06 | Bayer Ag | Isolierte Luziferasen Lu164, LuAL und Lu22, sowie deren Verwendung |
US6643635B2 (en) * | 2001-03-15 | 2003-11-04 | Sagemetrics Corporation | Methods for dynamically accessing, processing, and presenting data acquired from disparate data sources |
US8036939B2 (en) * | 2001-06-08 | 2011-10-11 | Servigistics, Inc. | Reporting in a supply chain |
US7587755B2 (en) * | 2004-07-02 | 2009-09-08 | Citrix Systems, Inc. | System and method for executing interactive applications with minimal privileges |
US20060230234A1 (en) * | 2005-03-30 | 2006-10-12 | Sap Ag. | Browser cache management |
US7779347B2 (en) * | 2005-09-02 | 2010-08-17 | Fourteen40, Inc. | Systems and methods for collaboratively annotating electronic documents |
US7831464B1 (en) * | 2006-04-06 | 2010-11-09 | ClearPoint Metrics, Inc. | Method and system for dynamically representing distributed information |
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2000
- 2000-11-23 DE DE10058091A patent/DE10058091A1/de not_active Withdrawn
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2001
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- 2001-11-12 AU AU2002220697A patent/AU2002220697A1/en not_active Withdrawn
- 2001-11-22 ES ES01994705T patent/ES2271111T3/es not_active Expired - Lifetime
- 2001-11-22 CN CN2012102094057A patent/CN102732537A/zh active Pending
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- 2001-11-22 KR KR1020037006911A patent/KR100841808B1/ko active IP Right Grant
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- 2001-11-22 WO PCT/EP2001/013597 patent/WO2002042470A1/de active IP Right Grant
- 2001-11-22 AU AU2002224876A patent/AU2002224876B2/en not_active Expired
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- 2001-11-22 CA CA2429451A patent/CA2429451C/en not_active Expired - Lifetime
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2003
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2004
- 2004-06-08 HK HK07103527.9A patent/HK1096423A1/xx not_active IP Right Cessation
- 2004-06-08 HK HK04104076A patent/HK1061044A1/xx not_active IP Right Cessation
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2011
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2012
- 2012-07-18 US US13/552,486 patent/US20130115641A1/en not_active Abandoned
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US5604123A (en) * | 1988-08-09 | 1997-02-18 | Toray Industries, Inc. | Luciferase, gene encoding the same and production process of the same |
WO1999049019A2 (en) * | 1998-03-27 | 1999-09-30 | Prolume, Ltd. | Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics |
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