KR100841808B1 - 단리된 루시퍼라제 및 그의 용도 - Google Patents
단리된 루시퍼라제 및 그의 용도 Download PDFInfo
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- KR100841808B1 KR100841808B1 KR1020037006911A KR20037006911A KR100841808B1 KR 100841808 B1 KR100841808 B1 KR 100841808B1 KR 1020037006911 A KR1020037006911 A KR 1020037006911A KR 20037006911 A KR20037006911 A KR 20037006911A KR 100841808 B1 KR100841808 B1 KR 100841808B1
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- South Korea
- Prior art keywords
- luciferase
- lys
- ala
- gly
- lual
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
- C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
- G01N2333/90241—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
Abstract
Description
박테리아 루시퍼라제 | |||||
유전자 산물 | 생물체 | 기질 | 파장 (λ) | 발현 | 참고문헌/특허 |
Lux 유전자 산물 (Lux Genes) | 비브리오 피스체리 (Vibrio fischerii) | FMN, 도데카날 (dodecanal), NADH | 495 nm | 세포질 | Apley et al., 1985 Gustafson G., US 5196524 |
코엘렌테라진 (Coelenterazine)-의존성 진핵생물 루시퍼라제 | |||||
유전자 산물 | 생물체 | 기질 | 파장 (λ) | 발현 | 참고문헌/특허 |
레닐라 (Renilla) 루시퍼라제 | 레닐라 레니포르미스 (Renilla reniformis) | 코엘렌테라진 | 480 nm | 세포질 | Mathews et al., 1977 Lorenz et al., 1991 Lorenz et al., 1996 Alan, P; WO 0020619 Milton J., US 5418155 Roelant C., WO 9938999 |
바르굴라 (Vargula)/ 시프리디아 (Cypridia) 루시퍼라제 | 바르굴라 힐겐도르페리 (Vargula hilgendorferii) | 바르굴라 루시페린 | 460 nm | 분비 | Thompson et al., 1989 Thompson et al., 1990 Tora, JP 05064583 Tora, JP 08027200 Renard et al., WO 9520653 |
와타세니아 (Watasenia) 루시퍼라제 | 와타세니아 신틸란스 (Watasenia scintillans) | 와타세니아 루시페린 | ? | 세포질 | Inoue et al., 1976 |
올로포러스 (Olophorus) 루시퍼라제 | 올로포러스 그라실리로스트리스 (Olophorus gracilirostris) | 코엘렌테라진 | 454 nm | 분비 | Inouye et al., 2000 |
에쿠오린 (Aequorin) | 에쿠오리아 에쿠오리아 (Aequoria aequoria) | 코엘렌테라진 (Ca2+로 활성화된 형태) | 470 nm | 세포질 | Head et al., 2000 Shimomura et al., 2000 Jones et al., 1999 Kendall et al., 1998 Inouye et al., 1985 Shimomura et al., 1969 Cormier et al., US 5798441 Cormier et al., US 5422266 |
오벨린 (Obelin) | 오벨리아 (Obelia) | 코엘렌테라진 | 470 nm | 세포질 | Matveev et al., 1999 Berestovskaya, 1999 |
코엘렌테라진-독립성 진핵생물 루시퍼라제 | |||||
유전자 산물 | 생물체 | 기질 | 파장 (λ) | 발현 | 참고문헌/특허 |
반딧불이 (firefly) 루시퍼라제 | 포티너스 피랄리스 (Photinus pyralis) | 반딧불이 루시페린, ATP | 550 nm | 세포질 | Webster et al., 1980 Gould et al., 1988 Sala-Newby et at.,1992 Bonin et al., 1994 Sherf B., US 5670356 KIKK, JP 09187281 |
Claims (8)
- 서열 5의 루시퍼라제 LuAL, 서열 1의 루시퍼라제 Lu164 또는 서열 3의 루시퍼라제 Lu22를 갖는 DNA 또는 RNA 분자.
- 제1항에 있어서, 상기 서열이 서열의 5' 위치에 기능적 프로모터를 갖는 분자.
- 제2항에 있어서, 재조합 DNA 또는 RNA 벡터의 한 성분인 분자.
- 제3항에 기재된 벡터를 함유한 생물체.
- 삭제
- 제1항의 뉴클레오티드 서열에 의해 코딩된 펩티드.
- 삭제
- 제1항 내지 제4항 및 제6항 중 어느 한 항에 기재된 루시퍼라제를 리포터 (reporter) 유전자로서 사용하는 방법.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10058091.2 | 2000-11-23 | ||
DE10058091A DE10058091A1 (de) | 2000-11-23 | 2000-11-23 | Isolierte Luziferasen Lu164, LuAL und Lu22, sowie deren Verwendung |
PCT/EP2001/013597 WO2002042470A1 (de) | 2000-11-23 | 2001-11-22 | Isolierte luziferasen sowie deren verwendung |
Publications (2)
Publication Number | Publication Date |
---|---|
KR20030051865A KR20030051865A (ko) | 2003-06-25 |
KR100841808B1 true KR100841808B1 (ko) | 2008-06-26 |
Family
ID=7664340
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020037006911A KR100841808B1 (ko) | 2000-11-23 | 2001-11-22 | 단리된 루시퍼라제 및 그의 용도 |
Country Status (19)
Country | Link |
---|---|
US (4) | US7297483B2 (ko) |
EP (1) | EP1339853B1 (ko) |
JP (2) | JP4179877B2 (ko) |
KR (1) | KR100841808B1 (ko) |
CN (4) | CN1847399B (ko) |
AT (1) | ATE335824T1 (ko) |
AU (3) | AU2002220697A1 (ko) |
BR (2) | BR0115534A (ko) |
CA (1) | CA2429451C (ko) |
DE (2) | DE10058091A1 (ko) |
DK (1) | DK1339853T3 (ko) |
ES (1) | ES2271111T3 (ko) |
HK (3) | HK1096423A1 (ko) |
IL (2) | IL155878A0 (ko) |
MX (1) | MXPA03004539A (ko) |
PL (1) | PL206385B1 (ko) |
PT (1) | PT1339853E (ko) |
WO (2) | WO2002042469A1 (ko) |
ZA (1) | ZA200303928B (ko) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10058091A1 (de) * | 2000-11-23 | 2002-06-06 | Bayer Ag | Isolierte Luziferasen Lu164, LuAL und Lu22, sowie deren Verwendung |
DE102007005804A1 (de) | 2007-02-06 | 2008-08-07 | Bayer Healthcare Ag | Sekretierte Luziferase Lu164M3 und deren Verwendung |
DE102007005803A1 (de) * | 2007-02-06 | 2008-08-07 | Bayer Healthcare Ag | Sekretierte Luziferase MLuc7 und deren Verwendung |
JP5605727B2 (ja) | 2010-04-06 | 2014-10-15 | Jnc株式会社 | セレンテラジン類縁体及びセレンテラミド類縁体 |
AU2011335901B2 (en) * | 2010-12-03 | 2016-12-15 | Gene Stream Pty Ltd | Improved light-emitting molecules |
US8435777B1 (en) | 2012-01-20 | 2013-05-07 | Cayla | CPG-free gene for a new secreted reporter protein |
CN104781401B (zh) | 2012-10-26 | 2018-06-05 | 独立行政法人产业技术综合研究所 | 人工生物发光酶 |
JP6132350B2 (ja) * | 2012-10-26 | 2017-05-24 | 国立研究開発法人産業技術総合研究所 | 人工生物発光酵素 |
BR112015013710B1 (pt) * | 2012-12-12 | 2022-08-09 | Promega Corporation | Métodos in vitro para detectar uma interação entre um agente bioativo e um alvo celular |
WO2015056762A1 (ja) * | 2013-10-18 | 2015-04-23 | 独立行政法人産業技術総合研究所 | 人工生物発光酵素に用いるための発光基質 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999049019A2 (en) * | 1998-03-27 | 1999-09-30 | Prolume, Ltd. | Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5604123A (en) * | 1988-08-09 | 1997-02-18 | Toray Industries, Inc. | Luciferase, gene encoding the same and production process of the same |
US5838313A (en) * | 1995-11-20 | 1998-11-17 | Siemens Corporate Research, Inc. | Multimedia-based reporting system with recording and playback of dynamic annotation |
US6853375B2 (en) * | 2000-05-10 | 2005-02-08 | Cognos Incorporated | Method for preemptive screen rendering |
DE10058091A1 (de) * | 2000-11-23 | 2002-06-06 | Bayer Ag | Isolierte Luziferasen Lu164, LuAL und Lu22, sowie deren Verwendung |
US6643635B2 (en) * | 2001-03-15 | 2003-11-04 | Sagemetrics Corporation | Methods for dynamically accessing, processing, and presenting data acquired from disparate data sources |
US8036939B2 (en) * | 2001-06-08 | 2011-10-11 | Servigistics, Inc. | Reporting in a supply chain |
US7587755B2 (en) * | 2004-07-02 | 2009-09-08 | Citrix Systems, Inc. | System and method for executing interactive applications with minimal privileges |
US20060230234A1 (en) * | 2005-03-30 | 2006-10-12 | Sap Ag. | Browser cache management |
US7779347B2 (en) * | 2005-09-02 | 2010-08-17 | Fourteen40, Inc. | Systems and methods for collaboratively annotating electronic documents |
US7831464B1 (en) * | 2006-04-06 | 2010-11-09 | ClearPoint Metrics, Inc. | Method and system for dynamically representing distributed information |
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2000
- 2000-11-23 DE DE10058091A patent/DE10058091A1/de not_active Withdrawn
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2001
- 2001-11-12 AU AU2002220697A patent/AU2002220697A1/en not_active Withdrawn
- 2001-11-12 WO PCT/EP2001/013057 patent/WO2002042469A1/de unknown
- 2001-11-22 PT PT01994705T patent/PT1339853E/pt unknown
- 2001-11-22 IL IL15587801A patent/IL155878A0/xx active IP Right Grant
- 2001-11-22 BR BR0115534-2A patent/BR0115534A/pt active IP Right Grant
- 2001-11-22 AU AU2487602A patent/AU2487602A/xx active Pending
- 2001-11-22 CN CN200510118849XA patent/CN1847399B/zh not_active Expired - Lifetime
- 2001-11-22 CN CN2012102094057A patent/CN102732537A/zh active Pending
- 2001-11-22 JP JP2002545175A patent/JP4179877B2/ja not_active Expired - Lifetime
- 2001-11-22 MX MXPA03004539A patent/MXPA03004539A/es active IP Right Grant
- 2001-11-22 WO PCT/EP2001/013597 patent/WO2002042470A1/de active IP Right Grant
- 2001-11-22 CN CNB018194338A patent/CN1303214C/zh not_active Expired - Lifetime
- 2001-11-22 DE DE50110723T patent/DE50110723D1/de not_active Expired - Lifetime
- 2001-11-22 CN CN2010105056541A patent/CN101979586B/zh not_active Expired - Lifetime
- 2001-11-22 EP EP01994705A patent/EP1339853B1/de not_active Expired - Lifetime
- 2001-11-22 DK DK01994705T patent/DK1339853T3/da active
- 2001-11-22 CA CA2429451A patent/CA2429451C/en not_active Expired - Lifetime
- 2001-11-22 AU AU2002224876A patent/AU2002224876B2/en not_active Expired
- 2001-11-22 ES ES01994705T patent/ES2271111T3/es not_active Expired - Lifetime
- 2001-11-22 PL PL362774A patent/PL206385B1/pl unknown
- 2001-11-22 AT AT01994705T patent/ATE335824T1/de active
- 2001-11-22 US US10/416,752 patent/US7297483B2/en not_active Expired - Lifetime
- 2001-11-22 KR KR1020037006911A patent/KR100841808B1/ko active IP Right Grant
- 2001-11-22 BR BRPI0115534-2A patent/BRPI0115534B1/pt unknown
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2003
- 2003-05-13 IL IL155878A patent/IL155878A/en unknown
- 2003-05-21 ZA ZA200303928A patent/ZA200303928B/en unknown
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2004
- 2004-06-08 HK HK07103527.9A patent/HK1096423A1/xx not_active IP Right Cessation
- 2004-06-08 HK HK04104076A patent/HK1061044A1/xx not_active IP Right Cessation
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- 2007-10-10 JP JP2007264492A patent/JP4519163B2/ja not_active Expired - Lifetime
- 2007-10-22 US US11/975,752 patent/US8236540B2/en active Active
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2011
- 2011-08-18 HK HK11108724.3A patent/HK1154623A1/xx not_active IP Right Cessation
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2012
- 2012-07-18 US US13/552,486 patent/US20130115641A1/en not_active Abandoned
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2015
- 2015-05-15 US US14/714,166 patent/US9745557B2/en not_active Expired - Lifetime
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999049019A2 (en) * | 1998-03-27 | 1999-09-30 | Prolume, Ltd. | Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics |
Non-Patent Citations (1)
Title |
---|
J. Bioluminescence and Chemiluminescence, Vol. 12(2), pp. 87-91 |
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