CN1847399B - 分离的荧光素酶及其用途 - Google Patents
分离的荧光素酶及其用途 Download PDFInfo
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- CN1847399B CN1847399B CN200510118849XA CN200510118849A CN1847399B CN 1847399 B CN1847399 B CN 1847399B CN 200510118849X A CN200510118849X A CN 200510118849XA CN 200510118849 A CN200510118849 A CN 200510118849A CN 1847399 B CN1847399 B CN 1847399B
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- China
- Prior art keywords
- luciferase
- lual
- pcdna3
- dna
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
- C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
- G01N2333/90241—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Ultra Sonic Daignosis Equipment (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
Description
基因产物 | 生物 | 底物 | λ | 表达 | 参考文献/专利 |
Lux基因 | Vibrio fischerii | FMN,十二烷醛NADH | 495nm | 细胞质 | Apley et al.,1985Gustafson G.,US5196524 |
基因产物 | 生物 | 底物 | λ | 表达 | 参考文献/专利 |
Renilla荧光素酶 | Renillareniformis | Coelenterazine | 480nm | 细胞质 | Mathews et al.,1977Lorenz et al,1991Lorenz et al.1996Alan,P;WO0020619Milton J.,US5418155Roelant C.,WO9938999 |
Vargula/Cypridia荧光素酶 | Vargulahilgendorferii | Vargula荧光素 | 460nm | 分泌 | Thomspon et al.,1989Thompson et al.,1990Tora,JP05064583Tora,JP08027200Renard et al.,WO9520653 |
Watasemia荧光素酶 | Wataseniascintillans | Watasemia荧光素 | ? | 细胞质 | Inoue et al.,1976 |
Olophorus荧光素酶 | Olophorusgracilirostris | Coelenterazine | 454 | 分泌 | Inouye et al,2000 |
水母蛋白 | Aequoriaaequoria | Coelenterazine(Ca<sup>2+</sup>激活的) | 470nm | 细胞质 | Headet al.2000Shimomura et al.,2000Jones et al.,1999Kendall et al.,1998Inouye et al.,1985Shimomura et al.,1969Cormier et al.,US5798441Cormier et al.,US5422266 |
Obelin | Obelia | Coelenterazine | 470nm | 细胞质 | Matveev et al.,1999Berestovskaya,1999 |
基因产物 | 生物 | 底物 | λ | 表达 | 参考文献 |
萤火虫荧光素酶 | Photinuspyralis | 萤火虫荧光素,ATP | 550nm | 细胞质 | Webster et al.,1980Gould et al.,1988Sala-Newby et al,1992Bonin et al.,1994SherfB.,US5670356KIKK,JP09187281 |
Claims (6)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10058091.2 | 2000-11-23 | ||
DE10058091A DE10058091A1 (de) | 2000-11-23 | 2000-11-23 | Isolierte Luziferasen Lu164, LuAL und Lu22, sowie deren Verwendung |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB018194338A Division CN1303214C (zh) | 2000-11-23 | 2001-11-22 | 分离的荧光素酶及其用途 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1847399A CN1847399A (zh) | 2006-10-18 |
CN1847399B true CN1847399B (zh) | 2010-12-15 |
Family
ID=7664340
Family Applications (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN200510118849XA Expired - Lifetime CN1847399B (zh) | 2000-11-23 | 2001-11-22 | 分离的荧光素酶及其用途 |
CN2012102094057A Pending CN102732537A (zh) | 2000-11-23 | 2001-11-22 | 分离的荧光素酶及其用途 |
CNB018194338A Expired - Lifetime CN1303214C (zh) | 2000-11-23 | 2001-11-22 | 分离的荧光素酶及其用途 |
CN2010105056541A Expired - Lifetime CN101979586B (zh) | 2000-11-23 | 2001-11-22 | 分离的荧光素酶及其用途 |
Family Applications After (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2012102094057A Pending CN102732537A (zh) | 2000-11-23 | 2001-11-22 | 分离的荧光素酶及其用途 |
CNB018194338A Expired - Lifetime CN1303214C (zh) | 2000-11-23 | 2001-11-22 | 分离的荧光素酶及其用途 |
CN2010105056541A Expired - Lifetime CN101979586B (zh) | 2000-11-23 | 2001-11-22 | 分离的荧光素酶及其用途 |
Country Status (19)
Country | Link |
---|---|
US (4) | US7297483B2 (zh) |
EP (1) | EP1339853B1 (zh) |
JP (2) | JP4179877B2 (zh) |
KR (1) | KR100841808B1 (zh) |
CN (4) | CN1847399B (zh) |
AT (1) | ATE335824T1 (zh) |
AU (3) | AU2002220697A1 (zh) |
BR (2) | BR0115534A (zh) |
CA (1) | CA2429451C (zh) |
DE (2) | DE10058091A1 (zh) |
DK (1) | DK1339853T3 (zh) |
ES (1) | ES2271111T3 (zh) |
HK (3) | HK1096423A1 (zh) |
IL (2) | IL155878A0 (zh) |
MX (1) | MXPA03004539A (zh) |
PL (1) | PL206385B1 (zh) |
PT (1) | PT1339853E (zh) |
WO (2) | WO2002042469A1 (zh) |
ZA (1) | ZA200303928B (zh) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10058091A1 (de) * | 2000-11-23 | 2002-06-06 | Bayer Ag | Isolierte Luziferasen Lu164, LuAL und Lu22, sowie deren Verwendung |
DE102007005803A1 (de) * | 2007-02-06 | 2008-08-07 | Bayer Healthcare Ag | Sekretierte Luziferase MLuc7 und deren Verwendung |
DE102007005804A1 (de) | 2007-02-06 | 2008-08-07 | Bayer Healthcare Ag | Sekretierte Luziferase Lu164M3 und deren Verwendung |
JP5605727B2 (ja) | 2010-04-06 | 2014-10-15 | Jnc株式会社 | セレンテラジン類縁体及びセレンテラミド類縁体 |
WO2012071631A1 (en) * | 2010-12-03 | 2012-06-07 | Gene Stream Pty Ltd | Improved light-emitting molecules |
US8435777B1 (en) | 2012-01-20 | 2013-05-07 | Cayla | CPG-free gene for a new secreted reporter protein |
JP6132350B2 (ja) * | 2012-10-26 | 2017-05-24 | 国立研究開発法人産業技術総合研究所 | 人工生物発光酵素 |
CN104781401B (zh) * | 2012-10-26 | 2018-06-05 | 独立行政法人产业技术综合研究所 | 人工生物发光酶 |
WO2014093677A1 (en) * | 2012-12-12 | 2014-06-19 | Promega Corporation | Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer |
JP6153140B2 (ja) * | 2013-10-18 | 2017-06-28 | 国立研究開発法人産業技術総合研究所 | 人工生物発光酵素に用いるための発光基質 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5604123A (en) * | 1988-08-09 | 1997-02-18 | Toray Industries, Inc. | Luciferase, gene encoding the same and production process of the same |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5838313A (en) * | 1995-11-20 | 1998-11-17 | Siemens Corporate Research, Inc. | Multimedia-based reporting system with recording and playback of dynamic annotation |
CA2324648C (en) * | 1998-03-27 | 2013-02-26 | Prolume, Ltd. | Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items |
US6995768B2 (en) * | 2000-05-10 | 2006-02-07 | Cognos Incorporated | Interactive business data visualization system |
DE10058091A1 (de) * | 2000-11-23 | 2002-06-06 | Bayer Ag | Isolierte Luziferasen Lu164, LuAL und Lu22, sowie deren Verwendung |
US6643635B2 (en) * | 2001-03-15 | 2003-11-04 | Sagemetrics Corporation | Methods for dynamically accessing, processing, and presenting data acquired from disparate data sources |
US8036939B2 (en) * | 2001-06-08 | 2011-10-11 | Servigistics, Inc. | Reporting in a supply chain |
US7587755B2 (en) * | 2004-07-02 | 2009-09-08 | Citrix Systems, Inc. | System and method for executing interactive applications with minimal privileges |
US20060230234A1 (en) * | 2005-03-30 | 2006-10-12 | Sap Ag. | Browser cache management |
US7779347B2 (en) * | 2005-09-02 | 2010-08-17 | Fourteen40, Inc. | Systems and methods for collaboratively annotating electronic documents |
US7831464B1 (en) * | 2006-04-06 | 2010-11-09 | ClearPoint Metrics, Inc. | Method and system for dynamically representing distributed information |
-
2000
- 2000-11-23 DE DE10058091A patent/DE10058091A1/de not_active Withdrawn
-
2001
- 2001-11-12 WO PCT/EP2001/013057 patent/WO2002042469A1/de unknown
- 2001-11-12 AU AU2002220697A patent/AU2002220697A1/en not_active Withdrawn
- 2001-11-22 BR BR0115534-2A patent/BR0115534A/pt active IP Right Grant
- 2001-11-22 CA CA2429451A patent/CA2429451C/en not_active Expired - Lifetime
- 2001-11-22 CN CN200510118849XA patent/CN1847399B/zh not_active Expired - Lifetime
- 2001-11-22 JP JP2002545175A patent/JP4179877B2/ja not_active Expired - Lifetime
- 2001-11-22 MX MXPA03004539A patent/MXPA03004539A/es active IP Right Grant
- 2001-11-22 CN CN2012102094057A patent/CN102732537A/zh active Pending
- 2001-11-22 KR KR1020037006911A patent/KR100841808B1/ko active IP Right Grant
- 2001-11-22 AU AU2002224876A patent/AU2002224876B2/en not_active Expired
- 2001-11-22 IL IL15587801A patent/IL155878A0/xx active IP Right Grant
- 2001-11-22 EP EP01994705A patent/EP1339853B1/de not_active Expired - Lifetime
- 2001-11-22 ES ES01994705T patent/ES2271111T3/es not_active Expired - Lifetime
- 2001-11-22 AU AU2487602A patent/AU2487602A/xx active Pending
- 2001-11-22 US US10/416,752 patent/US7297483B2/en not_active Expired - Lifetime
- 2001-11-22 AT AT01994705T patent/ATE335824T1/de active
- 2001-11-22 DK DK01994705T patent/DK1339853T3/da active
- 2001-11-22 PT PT01994705T patent/PT1339853E/pt unknown
- 2001-11-22 CN CNB018194338A patent/CN1303214C/zh not_active Expired - Lifetime
- 2001-11-22 CN CN2010105056541A patent/CN101979586B/zh not_active Expired - Lifetime
- 2001-11-22 DE DE50110723T patent/DE50110723D1/de not_active Expired - Lifetime
- 2001-11-22 PL PL362774A patent/PL206385B1/pl unknown
- 2001-11-22 BR BRPI0115534-2A patent/BRPI0115534B1/pt unknown
- 2001-11-22 WO PCT/EP2001/013597 patent/WO2002042470A1/de active IP Right Grant
-
2003
- 2003-05-13 IL IL155878A patent/IL155878A/en unknown
- 2003-05-21 ZA ZA200303928A patent/ZA200303928B/en unknown
-
2004
- 2004-06-08 HK HK07103527.9A patent/HK1096423A1/xx not_active IP Right Cessation
- 2004-06-08 HK HK04104076A patent/HK1061044A1/xx not_active IP Right Cessation
-
2007
- 2007-10-10 JP JP2007264492A patent/JP4519163B2/ja not_active Expired - Lifetime
- 2007-10-22 US US11/975,752 patent/US8236540B2/en active Active
-
2011
- 2011-08-18 HK HK11108724.3A patent/HK1154623A1/xx not_active IP Right Cessation
-
2012
- 2012-07-18 US US13/552,486 patent/US20130115641A1/en not_active Abandoned
-
2015
- 2015-05-15 US US14/714,166 patent/US9745557B2/en not_active Expired - Lifetime
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5604123A (en) * | 1988-08-09 | 1997-02-18 | Toray Industries, Inc. | Luciferase, gene encoding the same and production process of the same |
Non-Patent Citations (9)
Title |
---|
Thomson C M et al.The widespread occurrence and tissue distribution of theimidazolopyrazine luciferins.JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE12 2.1997,12(2),87-91. |
Thomson C M et al.The widespread occurrence and tissue distribution of theimidazolopyrazine luciferins.JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE12 2.1997,12(2),87-91. * |
王书锦 |
王今朝 |
蒋宇扬 |
蒋宇扬;金振华;王书锦;王今朝;马诚;蒋晓琳.萤火虫荧光素酶分离纯化及其特性的研究.清华大学学报(自然科学版)38 6.1998,38(6),16-19. * |
蒋晓琳.萤火虫荧光素酶分离纯化及其特性的研究.清华大学学报(自然科学版)38 6.1998,38(6),16-19. |
金振华 |
马诚 |
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