CN1195859C - 修饰化人源粒细胞-集落刺激因子及其制备方法 - Google Patents
修饰化人源粒细胞-集落刺激因子及其制备方法 Download PDFInfo
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Images
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/53—Colony-stimulating factor [CSF]
- C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
- A61P35/02—Antineoplastic agents specific for leukemia
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
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- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Description
转化子 | 实施例 | 表达载体 | 周质hG-CSF水平(mg/l) |
HM 10301 | 2(Step 4) | pT14SSG | 65 |
HM 10302 | 2(Step 7) | pT140SSG-4T22Q | 277 |
HM 10310 | 2(Step 3) | pT14SS1SG | 92 |
HM 10311 | 3 | pT14SS1S17SEG | 1,512 |
HM 10401 | 5 | pTOG | 85 |
HM 10409 | 4 | pTO1SG | 105 |
HM 10410 | 6(a) | pTO1S17SG | 1,477 |
HM 10411 | 6(b) | pTO17SG | 1,550 |
HM 10413 | 6(c) | pTO17TG | 1,373 |
HM 10414 | 6(d) | pTO17AG | 1,486 |
HM 10415 | 6(e) | pTO17GG | 1,480 |
HM 10416 | 6(f) | pTO17APG | 67 |
HM 10501 | 7(a) | pBADG | 54 |
HM 10510 | 7(b) | pBAD2M3VG | 69 |
HM 10511 | 7(c) | pBAD17SG | 937 |
HM 10512 | 7(d) | pBAD2M3V17SG | 983 |
Claims (18)
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KR1019990027418A KR100356140B1 (ko) | 1999-07-08 | 1999-07-08 | 인간 과립구 콜로니 자극인자 변이체 및 이의 생산 방법 |
KR1999/27418 | 1999-07-08 |
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CN1195859C true CN1195859C (zh) | 2005-04-06 |
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EP (1) | EP1194575B2 (zh) |
JP (1) | JP2003504069A (zh) |
KR (1) | KR100356140B1 (zh) |
CN (1) | CN1195859C (zh) |
AT (1) | ATE299187T1 (zh) |
AU (1) | AU757147C (zh) |
BR (2) | BRPI0012265B8 (zh) |
CA (1) | CA2378543C (zh) |
DE (1) | DE60021188T3 (zh) |
DK (1) | DK1194575T4 (zh) |
ES (1) | ES2243275T5 (zh) |
NZ (1) | NZ516476A (zh) |
PT (1) | PT1194575E (zh) |
RU (1) | RU2232772C2 (zh) |
WO (1) | WO2001004329A1 (zh) |
Families Citing this family (18)
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US6831158B2 (en) | 2000-01-10 | 2004-12-14 | Maxygen Holdings Ltd. | G-CSF conjugates |
CA2452582C (en) | 2001-07-11 | 2013-01-22 | Maxygen Holdings, Ltd. | G-csf conjugates |
DE60332358D1 (de) * | 2002-09-09 | 2010-06-10 | Hanall Pharmaceutical Co Ltd | Protease-resistente modifizierte interferon alpha polypeptide |
US8338373B2 (en) | 2003-10-24 | 2012-12-25 | Nora Therapeutics, Inc. | Method for reducing the risk of spontaneous abortion in a human female subject |
EP2140875A1 (en) * | 2003-10-24 | 2010-01-06 | Nora, LLC | Compositions and methods for healthy pregnancy |
US20090226397A1 (en) | 2003-10-24 | 2009-09-10 | Nora Therapeutics, Inc. | Compositions and methods for reducing the likelihood of implantation failure or miscarriage in recipients of artificial insemination |
AU2006254543A1 (en) | 2005-06-01 | 2006-12-07 | Maxygen Holdings Ltd. | PEGylated G-CSF polypeptides and methods of producing same |
DE102005033250A1 (de) | 2005-07-15 | 2007-01-18 | Bioceuticals Arzneimittel Ag | Verfahren zur Reinigung von G-CSF |
WO2009139784A1 (en) * | 2008-05-13 | 2009-11-19 | Nora Therapeutics, Inc. | Human g-csf analogs and methods of making and using thereof |
RU2519031C1 (ru) | 2010-01-19 | 2014-06-10 | Ханми Сайенс Ко., Лтд. | Жидкие препаративные формы для длительно действующего конъюгата g-csf |
US8980856B2 (en) | 2010-04-02 | 2015-03-17 | Curna, Inc. | Treatment of colony-stimulating factor 3 (CSF3) related diseases by inhibition of natural antisense transcript to CSF3 |
KR101831300B1 (ko) | 2010-10-29 | 2018-02-23 | 한미사이언스 주식회사 | 재조합 대장균으로부터 인간 과립구 콜로니 자극인자를 정제하는 방법 |
RU2467764C2 (ru) * | 2011-01-12 | 2012-11-27 | Государственное бюджетное образовательное учреждение дополнительного профессионального образования "Пензенский институт усовершенствования врачей" Министерства здравоохранения и социального развития Российской Федерации | Способ лечения больных аутоиммунной хронической крапивницей с использованием внутривенного введения препарата "габриглобин" |
WO2016013725A1 (ko) * | 2014-07-23 | 2016-01-28 | 주식회사 이큐스앤자루 | 과립구 콜로니 자극인자 변이 단백질 또는 이의 트랜스페린 융합 단백질을 유효성분으로 포함하는 약학적 조성물 |
KR101623906B1 (ko) | 2014-07-23 | 2016-05-24 | 주식회사 이큐스앤자루 | 과립구 콜로니 자극인자 변이 단백질 또는 이의 트랜스페린 융합 단백질을 유효성분으로 포함하는 약학적 조성물 |
BR112019016077A2 (pt) | 2017-02-03 | 2020-03-31 | Hanmi Pharm. Co., Ltd. | Conjugado da fórmula 1, método para preparar o conjugado, preparação de atuação longa, preparação para prevenir ou tratar diabetes e método para tratar diabetes |
US11684655B2 (en) | 2019-05-31 | 2023-06-27 | Spectrum Pharmaceuticals, Inc. | Methods of treating neutorpenia using G-CSF protein complex |
KR102375269B1 (ko) * | 2021-01-27 | 2022-03-17 | 한미약품 주식회사 | 단백질 액상 제제 및 이의 제조방법 |
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US4810643A (en) * | 1985-08-23 | 1989-03-07 | Kirin- Amgen Inc. | Production of pluripotent granulocyte colony-stimulating factor |
JPH01500483A (ja) * | 1986-08-11 | 1989-02-23 | シタス コーポレイション | G‐csf及びそのミューテインの発現 |
US5681720A (en) * | 1986-12-23 | 1997-10-28 | Kyowa Hakko Co., Ltd. | DNA encoding human granulocyte colony stimulating factor plasmids and host cells comprising same, and methods of expressing the encoded polypeptide |
US5362853A (en) * | 1986-12-23 | 1994-11-08 | Kyowa Hakko Kogyo Co., Ltd. | Polypeptide derivatives of human granulocyte colony stimulating factor |
JPH02104598A (ja) | 1988-04-12 | 1990-04-17 | Kirin Amgen Inc | ヒト顆粒球コロニー刺激因子ポリペプチド誘導体 |
NZ236819A (en) * | 1990-02-03 | 1993-07-27 | Max Planck Gesellschaft | Enzymatic cleavage of fusion proteins; fusion proteins; recombinant dna and pharmaceutical compositions |
GB9107846D0 (en) * | 1990-04-30 | 1991-05-29 | Ici Plc | Polypeptides |
EP0626448A3 (de) * | 1993-05-26 | 1998-01-14 | BOEHRINGER INGELHEIM INTERNATIONAL GmbH | Verfahren zur Herstellung und Reinigung von alpha-Interferon |
US6476198B1 (en) * | 1993-07-13 | 2002-11-05 | The Scripps Research Institute | Multispecific and multivalent antigen-binding polypeptide molecules |
US5451660A (en) * | 1993-12-13 | 1995-09-19 | Genentech, Inc. | Method for purifying polypeptides |
WO1996013599A1 (en) * | 1994-11-01 | 1996-05-09 | Winfried Wels | Nucleic acid transfer system |
US6100070A (en) * | 1995-10-05 | 2000-08-08 | G. D. Searle & Co. | G-CSF receptor agonists |
KR100316347B1 (ko) * | 1998-09-15 | 2002-08-27 | 한미약품(주) | 대장균엔테로톡신ⅱ신호펩티드의변형체와인체성장호르몬의융합단백질을발현하는재조합미생물및그를이용한인체성장호르몬의제조방법 |
JP4873818B2 (ja) * | 2000-05-16 | 2012-02-08 | ボルダー バイオテクノロジー, インコーポレイテッド | 遊離システイン残基を含有するタンパク質をリフォールディングする方法 |
-
1999
- 1999-07-08 KR KR1019990027418A patent/KR100356140B1/ko not_active IP Right Cessation
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2000
- 2000-07-07 WO PCT/KR2000/000733 patent/WO2001004329A1/en active IP Right Grant
- 2000-07-07 AU AU57106/00A patent/AU757147C/en not_active Expired
- 2000-07-07 PT PT00942494T patent/PT1194575E/pt unknown
- 2000-07-07 DK DK00942494T patent/DK1194575T4/da active
- 2000-07-07 EP EP00942494A patent/EP1194575B2/en not_active Expired - Lifetime
- 2000-07-07 DE DE60021188T patent/DE60021188T3/de not_active Expired - Lifetime
- 2000-07-07 BR BRPI0012265A patent/BRPI0012265B8/pt unknown
- 2000-07-07 AT AT00942494T patent/ATE299187T1/de active
- 2000-07-07 BR BR0012265-3A patent/BR0012265A/pt not_active IP Right Cessation
- 2000-07-07 CN CNB008100942A patent/CN1195859C/zh not_active Expired - Lifetime
- 2000-07-07 CA CA2378543A patent/CA2378543C/en not_active Expired - Lifetime
- 2000-07-07 NZ NZ516476A patent/NZ516476A/en not_active IP Right Cessation
- 2000-07-07 ES ES00942494T patent/ES2243275T5/es not_active Expired - Lifetime
- 2000-07-07 RU RU2002103159/13A patent/RU2232772C2/ru active
- 2000-07-07 JP JP2001509533A patent/JP2003504069A/ja active Pending
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2002
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2007
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KR100356140B1 (ko) | 2002-10-19 |
BRPI0012265B1 (pt) | 2018-11-21 |
ES2243275T5 (es) | 2009-12-02 |
WO2001004329A1 (en) | 2001-01-18 |
DE60021188D1 (de) | 2005-08-11 |
EP1194575A1 (en) | 2002-04-10 |
DE60021188T2 (de) | 2006-04-27 |
KR20010009171A (ko) | 2001-02-05 |
JP2003504069A (ja) | 2003-02-04 |
RU2232772C2 (ru) | 2004-07-20 |
AU757147B2 (en) | 2003-02-06 |
AU757147C (en) | 2005-03-03 |
AU5710600A (en) | 2001-01-30 |
US20040224393A1 (en) | 2004-11-11 |
EP1194575B2 (en) | 2009-08-26 |
DE60021188T3 (de) | 2010-04-01 |
DK1194575T4 (da) | 2009-12-07 |
BRPI0012265B8 (pt) | 2021-05-25 |
US20080064066A1 (en) | 2008-03-13 |
PT1194575E (pt) | 2005-10-31 |
BR0012265A (pt) | 2002-03-12 |
CA2378543C (en) | 2010-05-18 |
NZ516476A (en) | 2003-09-26 |
EP1194575B1 (en) | 2005-07-06 |
DK1194575T3 (da) | 2005-10-17 |
ES2243275T3 (es) | 2005-12-01 |
US7704709B2 (en) | 2010-04-27 |
EP1194575A4 (en) | 2002-10-24 |
CN1360636A (zh) | 2002-07-24 |
CA2378543A1 (en) | 2001-01-18 |
ATE299187T1 (de) | 2005-07-15 |
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