CN1161011C - Disease-free red orange seedling breeding method - Google Patents
Disease-free red orange seedling breeding method Download PDFInfo
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Abstract
The present invention relates to a disease-free red orange seedling breeding method. By heat treatment and drug treating, a high-standard parent garden, a cutting garden and a breeding garden are built; a sealed net greenhouse is adopted to enhance seedlings. The Lianjiang reddish orange seedlings bred with the present invention has no dangerous insect pests of citrus yellow shoot diseases, ulcers, etc., has the characteristics of thick seedlings, prosperous root systems, and yellowing ratio after 4 to 5 years of field planting of only 1 to 3% and greatly lower than that of traditional seedling raising ways, extends orchard economic service life, and raises yield, fruit quality and economic benefit.
Description
Technical field
The invention belongs to a kind of disease-free red orange seedling breeding method.
Background technology
Lianjiang is the township of Chinese blood orange, and the Lianjiang blood orange is best in quality, has won fame both at home and abroad, and is the characteristic fruit and the key product in Lianjiang City and even Guangdong Province.The eighties, Lianjiang City's blood orange production fast development becomes the pillar of whole city's rural economy.The early 1990s, big landslide appears in influence for various reasons, blood orange production.Wherein Hua Po one of the main reasons is that seedling is not tight to check on, adopts traditional seedling growing process, and promptly scion is gathered in the land for growing field crops, stock seed, scion are without any processing, it is poor that seedling ground isolates condition, and open country is grown seedlings etc., cultivates and plantation has the seedling of citrus yellow shoot disease and canker.The seedling that conventional art is cultivated is planted back 3-5, and the land for growing field crops xanthate ratio reaches 10-20%, even up to more than 30%, and canker generally takes place, and cause and use agricultural chemicals many, the cost height, fruit quality is poor, and the orchard economic life is short, deficiency in economic performance.
Technology contents
The purpose of this invention is to provide a kind of disease-free red orange seedling breeding method, by measures such as thermal treatment, drug treating and isolation epidemic prevention, high standard construction maternal plant garden, cutting orchard, propagation nursery, employing booth solarium inner sealing are grown seedlings, and cultivate the Lianjiang blood orange seedling of dangerous damage by disease and insect such as no citrus yellow shoot disease, canker.
The present invention includes following steps:
(1) select with growing seedlings, maternal plant garden will with other citrus fields apart from more than 5 kilometers, nursery lot then requires to establish enclosure wall and solarium at a distance of more than 3 kilometers, is aided with the parasitic plant of artificial removing citrus psylla again;
(2) anosis seedling is bred flow process, builds the gauze solarium, selects the base individual plant, carry out disinfection, detoxification, survey poison, through maternal plant garden, cutting orchard, anosis seedling propagation nursery, go out the garden again;
(1) builds the gauze solarium, comprise maternal plant garden, cutting orchard, three kinds of solariums of anosis seedling propagation nursery, the long 40-50 rice in solarium, wide 10-20 rice, maternal plant garden is high 3 meters, other 2.5 meters, at first, diameter 4.8cm iron pipe is fixed, with No. 9 an iron wire overcoat sebific ducts conduct netting twine with concrete every 4 meters, iron pipe is drawn mutually, according to solarium's size, with the wide 40-60 order gauze seam net of 1.5-2 rice, cover net then, and tight gauze with a linear slit, draw with reinforcing iron pipe, build 2 meters long at last, 1.5 meters wide two road net doors;
(2) set up maternal plant garden
A, graft disinfection, engrafting and cultivating, choose the branch of individual plant of few seed, the big high yield of fruit of the base seed selection of anosis blood orange and do scion, stay petiole, the water-bath of constant temperature graft disinfection case is heated, make the interior damp-heat air of case reach 48-49 ℃, scion is inverted in the disinfect box, add a cover rapidly, when treating that temperature rises to 48-49 ℃, pick up counting, keep 48-49 ℃, and kept 50 minutes, afterwards scion is taken out, put into the 700-800ppm streptomycin sulphate and add 1% alcohol liquid and soaked 30 minutes, taking-up cleans up, and grafts on the stock of having sterilized to cultivate.
Poison is surveyed in b, censorship, the seedling of above-mentioned cultivation is delivered to units concerned detect whether be with poison.
C, maternal plant garden are preserved, and do not introduce maternal plant garden with detecting with the plant of yellow twig and canker, and be potted plant in the solarium, and every basin 80 * 80 * 80cm, pedestal 30 * 100 * 100cm, every basin admix fertilizer 20-25kg, bran fertile 1.5-2kg, phosphate fertilizer 1-1.5kg.
(3) build cutting orchard, gauze is built the garden, chooses maternal plant garden scion engrafting and cultivating and forms, and nontoxic scion is provided, plantation seeding row spacing 80 * 100cm, thorough cut plantation, furrow width 60cm, dark 60cm, individual plant organic fertilizer 15-20kg, bran fertile 1-1.5kg, phosphate fertilizer 0.5-1kg.
(4) build anosis seedling propagation nursery, the field planting seedling is provided, grow seedlings with solarium and nutrition soil seedbed, the wide 90-100cm in seedbed, high 25-30cm, passage 40-50cm, nutrition soil is peat soil, pond sludge 50-55%, pig chicken manure 10-15%, bagasse 15-20%, river sand 15-18%, lime 1-2%, bran fertile 1-3%, phosphate fertilizer 1-3%, mix the seedbed thoroughly after above material stack retting becomes thoroughly decomposed, be implanted into the seedbed behind the suitable branch bed of stock and cultivate.
(3) cultivation of rootstock seedling
(1) selection of stock variety selects adaptability strong, and grafting affinity is strong, and red lemon that can the early fruiting and high yield high-quality, Jiangxi red tangerine seed are as the stock seed of Lianjiang blood orange.
(2) cultivation of rootstock seedling
A, seed thermal treatment, gather the seed of healthy tree, use gauze packed good after cleaning seed, place 50-52 ℃ of hot water preheating 10 minutes, take out and drop into immediately in the interior 54-56 ℃ of hot water of ultra thermostat, make water temperature remain within 54-56 ℃ lukewarm water and handled seed 50 minutes, intermittently stir, it is poly-cold that clear water is put in taking-up, dries, when non-caked, can the sowing of booth solarium.
B, construction seedbed, adopt solarium and nutrition soil seeding in nursery bed to grow seedlings, the wide 90-100cm in seedbed, high 20-25cm, passage 40-50cm, nutrition soil is peat soil, pond sludge 50-55%, pig chicken manure 10-15%, bagasse 15-20%, river sand 15-18%, lime 1-2%, bran fertile 1-3%, phosphate fertilizer 1-3%, mixes the seedbed thoroughly after above material stack retting becomes thoroughly decomposed.
C, sowing, with fenaminosulf or pcnb sterilization nursery soil, the sowing time is based on the autumn and winter before the sowing, red lemon seed is the 9-10 month, the Jiangxi red tangerine is the 12-1 month, broadcasts sowing, and is about to seed and evenly is sprinkling upon the furrow face, cover fine earth then or burn native 1-2cm, Winter-Spring low temperature is used the film cover seedbed, heat and moisture preserving, short seed germination growth, cultivate strong sprout, 666.7m to dredge to broadcast
2Seeding quantity is 30kg.
The management of d, rootstock seedling after planting, keeps ground moistening, and seedling begins fertilising when growing to the 3-5 leaf, fertilising in every month 1-2 time is based on thin liquid manure, when rootstock seedling grows to the 10-15 leaf, the gradable seedbed that is transplanted into anosis seedling propagation nursery, plantation seeding row spacing 10 * 20cm, every 666.7m
21.5 ten thousand strains are planted by the solarium; rich water quality management after strengthening transplanting and the forward and backward diseases and pests controlling of transplanting mainly are damping off, anthracnose, canker, two spotted spider mite, leaf miner, wood louse, the protection branch, and often weed a garden; promote growth, get final product grafting more than the 0.5-0.6cm when the stock trunk diameter reaches.
(4) cultivation of grafting
(1) collection of scion and processing, select cutting orchard to grow the branch strong, that eye is full as scion, defoliation is tied up the back and was soaked 2 hours with quadracycline 1000-1200ppm liquid, cleaning the back adds 1% alcohol liquid with streptomycin sulphate 700-800ppm and soaked 30 minutes, clean up grafting in time then.
(2) grafting period is spring and autumn and winter, and the spring connects the month into 1-3, and the autumn and winter connect the month into 9-11.
(3) adopt simple bud side grafting method
A, cut stock, before the grafting, stock is cut off, cleaning weeds, dry branches and fallen leaves at liftoff 15-18cm place.
B, cut scion, eye upwards, about 1cm place's roll setting is put down to the front upper place and is cut below eye, about 0.6cm place below eye then, with the miter angle cutter of splaying, about 0.4cm place's partial application above eye, standby with putting into the basin that fills clear water with wooden bud slightly.
C, the cutting stock, the stock belly overhead 10-14cm select at the place flat face, cut out straight smooth interface with cutter downwards along cambium, and bark cut half.
D, put scion, choosing is consistent with stock face size, and what length suited budders, and puts into interface, and both bevels are connected airtight relatively, is placed on a side of stock as the scion I, the cambium of a side is aligned connect airtight.
E, whipping film band are evenly whipped the graft union from bottom to top with plastic film tape, only reveal eye in order to germination.
(4) management after the grafting
A, mend and to connect, after the grafting ten days, check that the timely benefit that does not survive connects.
B, remove rudiment and bud thinning, in time the rudiment of stock is removed the minimizing nutrient consumption.Buddering growth back is dredged and is removed too much bud, keeps a strong bud as trunk, secondary tip 2-3 the branch of selecting and remain.
C, separate and tie up and cut anvil, after the grafting for the first time the tip aging after, with pocket knife film is removed, and the stock on the interface is cut with scissors.
D, reinforcement cultivation management often weed a garden, and keep the seedbed moistening, and arid is in time sprayed water.Survive Zhan Yehou, note thin execute bran water or composite fertilizer, promote the branch growth, strengthen diseases and pests controlling simultaneously, improve the quality of nursery stock.
Institute of the present invention breeding station is not with yellow twig and canker, and nursery stock is sturdy, well developed root system, and xanthate ratio is 1-3% only behind the field planting 4-5, grows seedlings well below tradition, prolongs the orchard economic life, improves output, fruit quality and economic benefit.
Embodiment
In the present invention, the basic principle of setting up anosis nursery is to select remote the isolation, select for use as far as possible and have natural isolation condition, maternal plant garden is set up in the place in no citrus in blocks garden in 1.5 kilometers on every side, and establish enclosure wall and solarium, and in the solarium, seal and grow seedlings, select the base individual plant, sterilization, detoxification, survey poison, through maternal plant garden, cutting orchard, anosis seedling propagation nursery, go out the garden again.Establish and improve the seedling-wood breeding archives, the detail record overall process of growing seedlings.Except carry out the booth solarium isolate grow seedlings, maternal plant garden, cutting orchard, the instrument of breeding the required usefulness in nursery must be special-purpose, can not use with the production orchard, citrus seed, nursery stock and the fruit forbidding to carry non-sterile enter the nursery; Strengthen passing the control of sick entomophila; Strict control personnel and other foreign material flow, and avoid importing into artificially the disease worm; Special messenger's operation, the clothing special use; Plant non-rutaceae fences around the nursery; The solarium establishes two sects, and turnover is closed the door, and the running check net is found the broken repairing in time of net, must sterilize before instrument such as secateurs, grafting knife uses, method is: clean with suds earlier, use flushing with clean water again, dry with clean paper then, be coated with for 1% clorox 1-3 second again, again with flushing with clean water and dry.Outplanting standard: do not have dangerous damage by disease and insect; Kind is pure, and stock is Jiangxi red tangerine or lemon, the healing of grafting mouth, and root system is complete, the prosperity of master root; The trunk end is straight, and growth is strong, and 2-3 second branch arranged.The one-level seedling is more than the thick 0.8cm in trunk footpath, more than the height of seedling 45cm, more than the thick 0.6cm in secondary seedling trunk footpath, more than the height of seedling 35cm.Visa goes out the garden, before the outplanting, carries out place of production quarantine and quality inspection by quality inspection personnel together with relevant professional and technical personnel, meets standard, signs and issues the place of production free pratique and the seedling quality quality certification, grants the garden.Nursery stock is answered classification, packs respectively by grade.Nursery stock wood kind/stock, grade, quantity, date, whereabouts (being grower place, name etc.) are provided with special record, and preservation files.
Embodiment 1
The branch of choosing anosis blood orange individual plant is as scion, and defoliation stays handle.The water-bath of constant temperature graft disinfection case is heated, make the interior damp-heat air of case reach 48.5 ℃, scion is inverted in the disinfect box, when treating that temperature rises to 48.5 ℃, pick up counting, keep 48.5 ± 0.3 ℃, and kept 50 minutes, taking-up changes the 700ppm streptomycin sulphate over to and adds 1% alcohol liquid and soaked 30 minutes, and taking-up cleans up, and in time is connected on the stock of having sterilized to cultivate.Poison is surveyed in the aging back censorship of the grafting secondary tip, is not with dangerous damage by disease and insect such as yellow twig, introduces the maternal plant garden field planting and preserves.
Embodiment 2
The seed of choosing the healthy tree of red lemon mid-September is as the stock seed, cleaning the back installs with mesh bag, place 50 ℃ of hot water preheatings 10 minutes, take out and put into 55 ℃ of hot water of ultra thermostat immediately, keep 55 ± 0.3 ℃ of lukewarm waters to handle seed 50 minutes, the clear water cooling is dried slightly, solarium's sowing, 666.7m
2Seeding quantity is 30kg.Rootstock seedling 10-15 leaf time stage moved into anosis seedling and bred in 40 meters * 10 meters of the solariums 20 centimetres of seeding row spacing 10 cm x, 666.7m mid-March
2Plant 1.5 ten thousand strains.Nutrition soil is peat soil, pond sludge 53%, pig chicken manure 10%, bagasse 15%, river sand 17%, lime 1%, bran fertilizer 2%, phosphate fertilizer 2%.Strengthen cultivation management, mid or late September the stock trunk diameter reach 0.5cm when above, choose the scion of cutting orchard, quadracycline liquid with 1000ppm soaked 2 hours, clean the back and add 1% alcohol liquid with the 750ppm streptomycin sulphate and soaked 30 minutes, clean up, in time use simple bud side grafting method engrafting and cultivating.After the nursery stock secondary tip is aging, quarantine and quality inspection, meet the garden standard.
Embodiment 8
The seed of choosing the healthy tree of Jiangxi red tangerine 12 the middle ten days is as the stock seed, cleaning the back installs with mesh bag, place 52 ℃ of hot water preheatings 10 minutes, take out and put into 55 ℃ of hot water of ultra thermostat immediately, keep 55 ± 0.3 ℃ of lukewarm waters to handle seed 50 minutes, the clear water cooling is dried slightly, solarium's sowing, 666.7m
2Sowing 30kg, the epiphragma insulation.Rootstock seedling 13-15 leaf time stage moved into anosis seedling and bred in 40 meters * 20 meters of the solariums 20 centimetres of seeding row spacing 10 cm x, 666.7m mid-May
2Plant 1.5 ten thousand strains.Nutrition soil is peat soil, pond sludge 50%, pig chicken manure 11%, bagasse 18%, river sand 15%, lime 1%, bran fertilizer 3%, phosphate fertilizer 2%.Strengthen cultivation management, late Febuary the stock trunk diameter reach 0.6cm when above, choose the scion of cutting orchard, quadracycline liquid with 1000ppm soaked 2 hours, clean the back and add 1% alcohol liquid with the 750ppm streptomycin sulphate and soaked 30 minutes, clean up, in time use simple bud side grafting method engrafting and cultivating.After the nursery stock secondary tip is aging, quarantine and quality inspection, meet the garden standard.
Claims (7)
1, a kind of disease-free red orange seedling breeding method is characterized in that comprising the steps:
(1) select with growing seedlings: maternal plant garden will with other citrus fields apart from more than 5 kilometers, nursery lot then requires to establish enclosure wall and solarium at a distance of more than 3 kilometers, is aided with the parasitic plant of artificial removing citrus psylla again;
(2) anosis seedling is bred flow process, builds the gauze solarium, selects the base individual plant, carry out disinfection, detoxification, survey poison, through maternal plant garden, cutting orchard, anosis seedling propagation nursery, go out the garden again;
(1) builds the gauze solarium, comprise maternal plant garden, cutting orchard, three kinds of solariums of anosis seedling propagation nursery, the long 40-50 rice in solarium, wide 10-20 rice, maternal plant garden is high 3 meters, other 2.5 meters, at first, diameter 4.8cm iron pipe is fixed, with No. 9 an iron wire overcoat sebific ducts conduct netting twine with concrete every 4 meters, iron pipe is drawn mutually, according to solarium's size, with the wide 40-60 order gauze seam net of 1.5-2 rice, cover net then, and tight gauze with a linear slit, draw with reinforcing iron pipe, build 2 meters long at last, 1.5 meters wide two road net doors;
(2) set up maternal plant garden
A, graft disinfection, engrafting and cultivating, choose the branch of anosis blood orange base seed selection individual plant and do scion, stay petiole, the water-bath of constant temperature graft disinfection case is heated, make the interior damp-heat air of case reach 48-49 ℃, scion is inverted in the disinfect box, add a cover rapidly, when treating that temperature rises to 48-49 ℃, pick up counting, keep 48-49 ℃, and kept 50 minutes, afterwards scion is taken out, put into the 700-800ppm streptomycin sulphate and add 1% alcohol liquid and soaked 30 minutes, taking-up cleans up, and grafts on the stock of having sterilized to cultivate;
Poison is surveyed in b, censorship, the seedling of above-mentioned cultivation is delivered to units concerned detect whether be with poison;
C, maternal plant garden are preserved, and do not introduce maternal plant garden with detecting with the plant of yellow twig and canker, and be potted plant in the solarium, and every basin 80 * 80 * 80cm, pedestal 30 * 100 * 100cm, every basin admix fertilizer 20-25kg, bran fertile 1.5-2kg, phosphate fertilizer 1-1.5kg;
(3) build cutting orchard, gauze is built the garden, chooses maternal plant garden scion engrafting and cultivating and forms, and nontoxic scion is provided, plantation seeding row spacing 80 * 100cm, thorough cut plantation, furrow width 60cm, dark 60cm, individual plant organic fertilizer 15-20kg, bran fertile 1-1.5kg, phosphate fertilizer 0.5-1kg;
(4) build anosis seedling propagation nursery, the field planting seedling is provided, grow seedlings with solarium and nutrition soil seedbed, the wide 90-100cm in seedbed, high 25-30cm, passage 40-50cm, nutrition soil is peat soil, pond sludge 50-55%, pig chicken manure 10-15%, bagasse 15-20%, river sand 15-18%, lime 1-2%, bran fertile 1-3%, phosphate fertilizer 1-3%, mix the seedbed thoroughly after above material stack retting becomes thoroughly decomposed, be implanted into the seedbed behind the suitable branch bed of stock and cultivate;
(3) cultivation of rootstock seedling
(1) stock variety is selected red lemon, the Jiangxi red tangerine seed stock seed as the Lianjiang blood orange;
(2) cultivation of rootstock seedling
A, seed thermal treatment, gather the seed of healthy tree, use gauze packed good after cleaning seed, place 50-52 ℃ of hot water preheating 10 minutes, take out and drop into immediately in the interior 54-56 ℃ of hot water of ultra thermostat, make water temperature remain within 54-56 ℃ lukewarm water and handled seed 50 minutes, intermittently stir, it is poly-cold that clear water is put in taking-up, dries, when non-caked, can sow the booth solarium;
B, construction seedbed, adopt solarium and nutrition soil seeding in nursery bed to grow seedlings, the wide 90-100cm in seedbed, high 20-25cm, passage 40-50cm, nutrition soil is peat soil, pond sludge 50-55%, pig chicken manure 10-15%, bagasse 15-20%, river sand 15-18%, lime 1-2%, bran fertile 1-3%, phosphate fertilizer 1-3%, mixes the seedbed thoroughly after above material stack retting becomes thoroughly decomposed;
C, sowing, sowing is preceding with fenaminosulf or pcnb sterilization nursery soil., the sowing time, red lemon seed was the 9-10 month based on the autumn and winter, the Jiangxi red tangerine is the 12-1 month, broadcast sowing, be about to seed and evenly be sprinkling upon the furrow face, cover fine earth then or burn native 1-2cm, Winter-Spring low temperature is used the film cover seedbed, heat and moisture preserving, short seed germination growth is cultivated strong sprout, 666.7m to dredge to broadcast
2Seeding quantity is 30kg;
The management of d, rootstock seedling after planting, keeps ground moistening, and seedling begins fertilising when growing to the 3-5 leaf, fertilising in every month 1-2 time is based on thin liquid manure, when rootstock seedling grows to the 10-15 leaf, the gradable seedbed that is transplanted into anosis seedling propagation nursery, plantation seeding row spacing 10 * 20cm, every 666.7m
21.5 ten thousand strains are planted by the solarium, and rich water quality management after strengthening transplanting and the forward and backward diseases and pests controlling of transplanting mainly are damping off, anthracnose, canker, two spotted spider mite, leaf miner, wood louse, the protection branch, and often weed a garden, promote growth, when reaching, the stock trunk diameter gets final product grafting more than the 0.5-0.6cm;
(4) cultivation of grafting
(1) collection of scion and processing, select cutting orchard to grow the branch strong, that eye is full as scion, defoliation is tied up the back and was soaked 2 hours with quadracycline 1000-1200ppm liquid, cleaning the back adds 1% alcohol liquid with streptomycin sulphate 700-800ppm and soaked 30 minutes, clean up grafting in time then;
(2) grafting period is spring and autumn and winter, and the spring connects the month into 1-3, and the autumn and winter connect the month into 9-11;
(3) adopt simple bud side grafting method
A, cut stock, before the grafting, stock is cut off, cleaning weeds, dry branches and fallen leaves at liftoff 15-18cm place;
B, cut scion, eye upwards, about 1cm place's roll setting is put down to the front upper place and is cut below eye, about 0.6cm place below eye then, with the miter angle cutter of splaying, about 0.4cm place's partial application above eye, standby with putting into the basin that fills clear water with wooden bud slightly;
C, the cutting stock, the stock belly overhead 10-14cm select at the place flat face, cut out straight smooth interface with cutter downwards along cambium, and bark cut half;
D, put scion, choosing is consistent with stock face size, and what length suited budders, and puts into interface, and both bevels are connected airtight relatively, is placed on a side of stock as the scion I, and the cambium of a side is aligned connect airtight,
E, whipping film band are evenly whipped the graft union from bottom to top with plastic film tape, only reveal eye in order to germination;
(4) management after the grafting
A, mend and to connect, after the grafting ten days, check that the timely benefit that does not survive connects;
B, remove rudiment and bud thinning, in time the rudiment of stock is removed, reduce nutrient consumption, budder that the growth back is thin removes too much bud, keep a strong bud, secondary tip 2-3 the branch of selecting and remain as trunk;
C, separate and tie up and cut anvil, after the grafting for the first time the tip aging after, with pocket knife film is removed, and the stock on the interface is cut with scissors;
D, reinforcement cultivation management often weed a garden, and keep the seedbed moistening, and arid is in time sprayed water, and survives Zhan Yehou, note thin executes bran water or composite fertilizer, promote branch to grow, and strengthen diseases and pests controlling simultaneously, improve the quality of nursery stock;
2, according to the described disease-free red orange seedling breeding method of claim 1, it is characterized in that: the place in no citrus in blocks garden in being chosen on every side 1.5 kilometers with growing seedlings, establish the sealing of enclosure wall and gauze solarium and grow seedlings.
3, according to the described disease-free red orange seedling breeding method of claim 1, it is characterized in that: plant non-rutaceae fences around the nursery, before using, instrument such as secateurs, grafting knife must sterilize, method is: clean with suds earlier, use flushing with clean water again, dry with clean paper then, be coated with for 1% clorox 1-3 second again, again with flushing with clean water and dry.
4, according to the described disease-free red orange seedling breeding method of claim 1, it is characterized in that: the outplanting standard: do not have dangerous damage by disease and insect; Kind is pure, and stock is Jiangxi red tangerine or red lemon, the healing of grafting mouth, and root system is complete, the prosperity of master root; The trunk end is straight, and 2-3 second branch arranged, and the one-level seedling is more than the thick 0.8cm of trunk stem, more than the height of seedling 45cm, more than the thick 0.6cm of secondary seedling trunk stem, more than the height of seedling 35cm.
5, according to the described disease-free red orange seedling breeding method of claim 1, it is characterized in that: the branch of choosing anosis blood orange individual plant is as scion, defoliation stays handle, the water-bath of constant temperature graft disinfection case is heated, make the interior damp-heat air of case reach 48.5 ℃, scion is inverted in the disinfect box, when treating that temperature rises to 48.5 ℃, picks up counting, keep 48.5 ± 0.3 ℃, and kept 50 minutes, to take out and to change the 700ppm streptomycin sulphate over to and add 1% alcohol liquid and soaked 30 minutes, taking-up cleans up, in time be connected on the stock of having sterilized and cultivate, poison is surveyed in the aging back censorship of the grafting secondary tip, is not with dangerous damage by disease and insect such as yellow twig, introduces the maternal plant garden field planting and preserves.
6, according to the described disease-free red orange seedling breeding method of claim 1, it is characterized in that: the seed of choosing the healthy tree of red lemon mid-September is as the stock seed, cleaning the back installs with mesh bag, place 50 ℃ of hot water preheatings 10 minutes, take out and put into 55 ℃ of hot water of ultra thermostat immediately, keep 55 ± 0.3 ℃ of lukewarm waters to handle seed 50 minutes, the clear water cooling, dry solarium's sowing, 666.7m slightly
2Seeding quantity is 30kg, and rootstock seedling 10-15 leaf time stage moved into anosis seedling and bred in 40 meters * 10 meters of the solariums 20 centimetres of seeding row spacing 10 cm x, 666.7m mid-March
2Plant 1.5 ten thousand strains, nutrition soil is peat soil, pond sludge 53%, pig chicken manure 10%, bagasse 15%, river sand 17%, lime 1%, bran fertilizer 2%, phosphate fertilizer 2%, strengthen cultivation management, mid-September, the stock trunk diameter reached 0.5cm when above, choose the scion of cutting orchard, quadracycline liquid with 1000ppm soaked 2 hours, cleaning the back adds 1% alcohol liquid with the 750ppm streptomycin sulphate and soaked 30 minutes, clean up, in time with simple bud side grafting method engrafting and cultivating, after the nursery stock secondary tip is aging, quarantine and quality inspection, meet the garden standard.
7, according to the described disease-free red orange seedling breeding method of claim 1, it is characterized in that: the seed of choosing the healthy tree of Jiangxi red tangerine 12 the middle ten days is as the stock seed, cleaning the back installs with mesh bag, place 52 ℃ of hot water preheatings 10 minutes, take out and put into 55 ℃ of hot water of ultra thermostat immediately, kept 0.3 ℃ of lukewarm water processing of 55 soil and son 50 minutes, the clear water cooling, dry solarium's sowing, 666.7m slightly
2Sowing 30kg, epiphragma insulation, rootstock seedling 13-15 leaf time stage moved into anosis seedling and bred in 40 meters * 20 meters of the solariums 20 centimetres of seeding row spacing 10 cm x, 666.7m mid-May
2Plant 1.5 ten thousand strains, nutrition soil is peat soil, pond sludge 50%, pig chicken manure 11%, bagasse 18%, river sand 15%, lime 1%, bran fertilizer 3%, phosphate fertilizer 2%, strengthen cultivation management, late Febuary, the stock scapus reached 0.6cm when above, choose the scion of cutting orchard, quadracycline liquid with 1000ppm soaked 2 hours, cleaning the back adds 1% alcohol liquid with the 750ppm streptomycin sulphate and soaked 30 minutes, clean up, in time with simple bud side grafting method engrafting and cultivating, after the nursery stock secondary tip is aging, quarantine and quality inspection, meet the garden standard.
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