CN111819280A - 发夹型单链rna分子的制备方法 - Google Patents

发夹型单链rna分子的制备方法 Download PDF

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Publication number
CN111819280A
CN111819280A CN201980019534.XA CN201980019534A CN111819280A CN 111819280 A CN111819280 A CN 111819280A CN 201980019534 A CN201980019534 A CN 201980019534A CN 111819280 A CN111819280 A CN 111819280A
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stranded
linker
molecule
rna
seq
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稻田英朗
伊关克彦
冲村庆一
佐野坂真人
A·高科
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Toray Industries Inc
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Toray Industries Inc
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1136—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against growth factors, growth regulators, cytokines, lymphokines or hormones
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/122—Hairpin
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/318—Chemical structure of the backbone where the PO2 is completely replaced, e.g. MMI or formacetal
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
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    • C12N2330/30—Production chemically synthesised

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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CN201980019534.XA 2018-03-30 2019-03-29 发夹型单链rna分子的制备方法 Pending CN111819280A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2018070423 2018-03-30
JP2018-070423 2018-03-30
PCT/JP2019/013923 WO2019189722A1 (ja) 2018-03-30 2019-03-29 ヘアピン型一本鎖ｒｎａ分子の製造方法

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CN111819280A true CN111819280A (zh) 2020-10-23

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US (2) US11920131B2 (https=)
EP (1) EP3778886A4 (https=)
JP (2) JP6631751B1 (https=)
KR (1) KR20200136363A (https=)
CN (1) CN111819280A (https=)
AU (1) AU2019242331A1 (https=)
BR (1) BR112020017769A2 (https=)
CA (1) CA3094160A1 (https=)
IL (1) IL277346A (https=)
MX (1) MX2020009556A (https=)
PH (1) PH12020551487A1 (https=)
RU (1) RU2020130258A (https=)
SA (1) SA520420072B1 (https=)
TW (1) TWI772632B (https=)
WO (1) WO2019189722A1 (https=)
ZA (1) ZA202005149B (https=)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023024500A1 (en) * 2021-08-27 2023-03-02 Peking University Constructs and methods for preparing circular rna
CN115774075A (zh) * 2023-02-15 2023-03-10 江苏耀海生物制药有限公司 一种基于RP-HPLC分析体外转录产物成分circRNA的方法
WO2024051143A1 (zh) * 2022-09-08 2024-03-14 凯莱英医药集团（天津）股份有限公司 利用rna连接酶制备寡核苷酸的方法

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI848981B (zh) 2018-10-02 2024-07-21 日商東麗股份有限公司 新穎亞磷醯胺、及髮夾型單股ｒｎａ分子之製造方法
EP4069838A1 (en) * 2019-12-03 2022-10-12 Beam Therapeutics Inc. Synthetic guide rna, compositions, methods, and uses thereof
JP7613130B2 (ja) * 2020-03-25 2025-01-15 味の素株式会社 リガーゼ変異体
US12522629B2 (en) 2020-03-27 2026-01-13 Sumitomo Chemical Company, Limited Method for producing nucleic acid oligomer

Citations (4)

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US20040058886A1 (en) * 2002-08-08 2004-03-25 Dharmacon, Inc. Short interfering RNAs having a hairpin structure containing a non-nucleotide loop
WO2013077446A1 (ja) * 2011-11-26 2013-05-30 株式会社ボナック 遺伝子発現制御のための一本鎖核酸分子
WO2017035090A1 (en) * 2015-08-21 2017-03-02 Thomas Jefferson University Dumbbell-pcr: a method to quantify specific small rna variants with a single nucleotide resolution at terminal sequences
WO2017073767A1 (ja) * 2015-10-30 2017-05-04 株式会社ボナック ＴＧＦ－β１遺伝子の発現を抑制する一本鎖核酸分子を安定に含有する組成物

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WO2001040516A2 (en) 1999-12-02 2001-06-07 Molecular Staging Inc. Generation of single-strand circular dna from linear self-annealing segments
JP5296328B2 (ja) * 2007-05-09 2013-09-25 独立行政法人理化学研究所 １本鎖環状ｒｎａおよびその製造方法
EP2256191A4 (en) * 2008-02-15 2011-07-06 Riken CYCLIC SINGLE NUCLEIC ACID COMPLEX AND METHOD FOR THE PRODUCTION THEREOF
JPWO2011052013A1 (ja) 2009-10-29 2013-03-14 株式会社バイオダイナミクス研究所 核酸鎖の結合および修飾方法
HUE037500T2 (hu) 2010-07-08 2018-08-28 Bonac Corp Egyszálú nukleinsav molekula a génexpresszió szabályozására
ES2527660T3 (es) 2010-08-03 2015-01-28 Bonac Corporation Molécula de ARN monocatenario que tiene esqueleto alicíclico que contiene nitrógeno
WO2013027843A1 (ja) 2011-08-25 2013-02-28 株式会社ボナック 配糖体化合物、チオエーテルの製造方法、エーテル、エーテルの製造方法、配糖体化合物の製造方法、核酸の製造方法
US9528111B2 (en) 2012-01-07 2016-12-27 Bonac Corporation Single-stranded nucleic acid molecule having amino acid backbone
US9816120B2 (en) 2013-01-09 2017-11-14 The Penn State Research Foundation Low sequence bias single-stranded DNA ligation
KR20170129264A (ko) 2015-04-02 2017-11-24 가부시키가이샤 보낙 글리코시드 화합물의 제조 방법
GB201612011D0 (en) 2016-07-11 2016-08-24 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides
JP6815601B2 (ja) 2016-11-01 2021-01-20 スタンレー電気株式会社 ウルツ鉱構造のＺｎＯＳ混晶粒子の製造方法
EP3604528A4 (en) * 2017-03-31 2021-01-13 Bonac Corporation CYCLIC NUCLEIC ACID MOLECULE WITH GENE EXPRESSION CONTROL FUNCTION
TWI830718B (zh) * 2018-02-09 2024-02-01 日商住友化學股份有限公司 核酸分子之製造方法
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Patent Citations (4)

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Publication number Priority date Publication date Assignee Title
US20040058886A1 (en) * 2002-08-08 2004-03-25 Dharmacon, Inc. Short interfering RNAs having a hairpin structure containing a non-nucleotide loop
WO2013077446A1 (ja) * 2011-11-26 2013-05-30 株式会社ボナック 遺伝子発現制御のための一本鎖核酸分子
WO2017035090A1 (en) * 2015-08-21 2017-03-02 Thomas Jefferson University Dumbbell-pcr: a method to quantify specific small rna variants with a single nucleotide resolution at terminal sequences
WO2017073767A1 (ja) * 2015-10-30 2017-05-04 株式会社ボナック ＴＧＦ－β１遺伝子の発現を抑制する一本鎖核酸分子を安定に含有する組成物

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023024500A1 (en) * 2021-08-27 2023-03-02 Peking University Constructs and methods for preparing circular rna
WO2024051143A1 (zh) * 2022-09-08 2024-03-14 凯莱英医药集团（天津）股份有限公司 利用rna连接酶制备寡核苷酸的方法
CN115774075A (zh) * 2023-02-15 2023-03-10 江苏耀海生物制药有限公司 一种基于RP-HPLC分析体外转录产物成分circRNA的方法

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PH12020551487A1 (en) 2021-08-23
ZA202005149B (en) 2022-01-26
EP3778886A1 (en) 2021-02-17
BR112020017769A2 (pt) 2021-01-05
RU2020130258A (ru) 2022-05-04
US11920131B2 (en) 2024-03-05
SA520420072B1 (ar) 2024-04-21
JPWO2019189722A1 (ja) 2020-04-30
US20210024930A1 (en) 2021-01-28
JP6631751B1 (ja) 2020-01-15
TW202003842A (zh) 2020-01-16
KR20200136363A (ko) 2020-12-07
AU2019242331A1 (en) 2020-09-10
EP3778886A4 (en) 2022-11-02
JP2019213567A (ja) 2019-12-19
TWI772632B (zh) 2022-08-01
JP7363316B2 (ja) 2023-10-18
US20240167033A1 (en) 2024-05-23
MX2020009556A (es) 2020-10-05
CA3094160A1 (en) 2019-10-03
WO2019189722A1 (ja) 2019-10-03
IL277346A (en) 2020-10-29

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