KR20200136363A - 헤어핀형 1개쇄 rna 분자의 제조 방법 - Google Patents

헤어핀형 1개쇄 rna 분자의 제조 방법 Download PDF

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KR20200136363A
KR20200136363A KR1020207018860A KR20207018860A KR20200136363A KR 20200136363 A KR20200136363 A KR 20200136363A KR 1020207018860 A KR1020207018860 A KR 1020207018860A KR 20207018860 A KR20207018860 A KR 20207018860A KR 20200136363 A KR20200136363 A KR 20200136363A
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rna molecule
linker
stranded oligo
oligo rna
stranded
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히데아키 이나다
카츠히코 이세키
케이이치 오키무라
마사토 사노사카
아유미 타카시나
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도레이 카부시키가이샤
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12Y605/01003—RNA ligase (ATP) (6.5.1.3)
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1136—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against growth factors, growth regulators, cytokines, lymphokines or hormones
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/122—Hairpin
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/318—Chemical structure of the backbone where the PO2 is completely replaced, e.g. MMI or formacetal
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
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    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised

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  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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KR1020207018860A 2018-03-30 2019-03-29 헤어핀형 1개쇄 rna 분자의 제조 방법 Pending KR20200136363A (ko)

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Application Number Priority Date Filing Date Title
JPJP-P-2018-070423 2018-03-30
JP2018070423 2018-03-30
PCT/JP2019/013923 WO2019189722A1 (ja) 2018-03-30 2019-03-29 ヘアピン型一本鎖ｒｎａ分子の製造方法

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KR20200136363A true KR20200136363A (ko) 2020-12-07

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US (2) US11920131B2 (https=)
EP (1) EP3778886A4 (https=)
JP (2) JP6631751B1 (https=)
KR (1) KR20200136363A (https=)
CN (1) CN111819280A (https=)
AU (1) AU2019242331A1 (https=)
BR (1) BR112020017769A2 (https=)
CA (1) CA3094160A1 (https=)
IL (1) IL277346A (https=)
MX (1) MX2020009556A (https=)
PH (1) PH12020551487A1 (https=)
RU (1) RU2020130258A (https=)
SA (1) SA520420072B1 (https=)
TW (1) TWI772632B (https=)
WO (1) WO2019189722A1 (https=)
ZA (1) ZA202005149B (https=)

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TWI848981B (zh) 2018-10-02 2024-07-21 日商東麗股份有限公司 新穎亞磷醯胺、及髮夾型單股ｒｎａ分子之製造方法
EP4069838A1 (en) * 2019-12-03 2022-10-12 Beam Therapeutics Inc. Synthetic guide rna, compositions, methods, and uses thereof
JP7613130B2 (ja) * 2020-03-25 2025-01-15 味の素株式会社 リガーゼ変異体
US12522629B2 (en) 2020-03-27 2026-01-13 Sumitomo Chemical Company, Limited Method for producing nucleic acid oligomer
KR20240046780A (ko) * 2021-08-27 2024-04-09 페킹 유니버시티 원형 rna를 제조하기 위한 구축물 및 방법
CN117660374A (zh) * 2022-09-08 2024-03-08 凯莱英医药集团（天津）股份有限公司 Rna连接酶在寡核苷酸制备上的应用
CN115774075B (zh) * 2023-02-15 2023-06-06 江苏耀海生物制药有限公司 一种基于RP-HPLC分析体外转录产物成分circRNA的方法

Citations (4)

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US20040058886A1 (en) 2002-08-08 2004-03-25 Dharmacon, Inc. Short interfering RNAs having a hairpin structure containing a non-nucleotide loop
WO2011052013A1 (ja) 2009-10-29 2011-05-05 株式会社バイオダイナミクス研究所 核酸鎖の結合および修飾方法
WO2013027843A1 (ja) 2011-08-25 2013-02-28 株式会社ボナック 配糖体化合物、チオエーテルの製造方法、エーテル、エーテルの製造方法、配糖体化合物の製造方法、核酸の製造方法
WO2016159374A1 (ja) 2015-04-02 2016-10-06 株式会社ボナック 配糖体化合物の製造方法

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JP5296328B2 (ja) * 2007-05-09 2013-09-25 独立行政法人理化学研究所 １本鎖環状ｒｎａおよびその製造方法
EP2256191A4 (en) * 2008-02-15 2011-07-06 Riken CYCLIC SINGLE NUCLEIC ACID COMPLEX AND METHOD FOR THE PRODUCTION THEREOF
HUE037500T2 (hu) 2010-07-08 2018-08-28 Bonac Corp Egyszálú nukleinsav molekula a génexpresszió szabályozására
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US20040058886A1 (en) 2002-08-08 2004-03-25 Dharmacon, Inc. Short interfering RNAs having a hairpin structure containing a non-nucleotide loop
WO2011052013A1 (ja) 2009-10-29 2011-05-05 株式会社バイオダイナミクス研究所 核酸鎖の結合および修飾方法
WO2013027843A1 (ja) 2011-08-25 2013-02-28 株式会社ボナック 配糖体化合物、チオエーテルの製造方法、エーテル、エーテルの製造方法、配糖体化合物の製造方法、核酸の製造方法
WO2016159374A1 (ja) 2015-04-02 2016-10-06 株式会社ボナック 配糖体化合物の製造方法

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PH12020551487A1 (en) 2021-08-23
ZA202005149B (en) 2022-01-26
EP3778886A1 (en) 2021-02-17
BR112020017769A2 (pt) 2021-01-05
RU2020130258A (ru) 2022-05-04
US11920131B2 (en) 2024-03-05
SA520420072B1 (ar) 2024-04-21
JPWO2019189722A1 (ja) 2020-04-30
US20210024930A1 (en) 2021-01-28
JP6631751B1 (ja) 2020-01-15
TW202003842A (zh) 2020-01-16
AU2019242331A1 (en) 2020-09-10
EP3778886A4 (en) 2022-11-02
CN111819280A (zh) 2020-10-23
JP2019213567A (ja) 2019-12-19
TWI772632B (zh) 2022-08-01
JP7363316B2 (ja) 2023-10-18
US20240167033A1 (en) 2024-05-23
MX2020009556A (es) 2020-10-05
CA3094160A1 (en) 2019-10-03
WO2019189722A1 (ja) 2019-10-03
IL277346A (en) 2020-10-29

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