SA520420072B1 - Rna طريقة لإنتاج جزيء وحيد الجديلة دبوسي - Google Patents
Rna طريقة لإنتاج جزيء وحيد الجديلة دبوسيInfo
- Publication number
- SA520420072B1 SA520420072B1 SA520420072A SA520420072A SA520420072B1 SA 520420072 B1 SA520420072 B1 SA 520420072B1 SA 520420072 A SA520420072 A SA 520420072A SA 520420072 A SA520420072 A SA 520420072A SA 520420072 B1 SA520420072 B1 SA 520420072B1
- Authority
- SA
- Saudi Arabia
- Prior art keywords
- stranded
- stranded oligorna
- rna molecule
- stranded rna
- oligorna molecule
- Prior art date
Links
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 238000000137 annealing Methods 0.000 abstract 2
- 230000014509 gene expression Effects 0.000 abstract 2
- 230000002401 inhibitory effect Effects 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 101001095872 Enterobacteria phage T4 RNA ligase 2 Proteins 0.000 abstract 1
- 108090000364 Ligases Proteins 0.000 abstract 1
- 102000003960 Ligases Human genes 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y605/00—Ligases forming phosphoric ester bonds (6.5)
- C12Y605/01—Ligases forming phosphoric ester bonds (6.5) forming phosphoric ester bonds (6.5.1)
- C12Y605/01003—RNA ligase (ATP) (6.5.1.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C12N15/1136—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against growth factors, growth regulators, cytokines, lymphokines or hormones
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
- C12N2310/122—Hairpin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/31—Chemical structure of the backbone
- C12N2310/318—Chemical structure of the backbone where the PO2 is completely replaced, e.g. MMI or formacetal
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/53—Physical structure partially self-complementary or closed
- C12N2310/531—Stem-loop; Hairpin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2330/00—Production
- C12N2330/30—Production chemically synthesised
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Endocrinology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2018070423 | 2018-03-30 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SA520420072B1 true SA520420072B1 (ar) | 2024-04-21 |
Family
ID=68061953
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SA520420072A SA520420072B1 (ar) | 2018-03-30 | 2020-08-31 | Rna طريقة لإنتاج جزيء وحيد الجديلة دبوسي |
Country Status (16)
| Country | Link |
|---|---|
| US (2) | US11920131B2 (https=) |
| EP (1) | EP3778886A4 (https=) |
| JP (2) | JP6631751B1 (https=) |
| KR (1) | KR20200136363A (https=) |
| CN (1) | CN111819280A (https=) |
| AU (1) | AU2019242331A1 (https=) |
| BR (1) | BR112020017769A2 (https=) |
| CA (1) | CA3094160A1 (https=) |
| IL (1) | IL277346A (https=) |
| MX (1) | MX2020009556A (https=) |
| PH (1) | PH12020551487A1 (https=) |
| RU (1) | RU2020130258A (https=) |
| SA (1) | SA520420072B1 (https=) |
| TW (1) | TWI772632B (https=) |
| WO (1) | WO2019189722A1 (https=) |
| ZA (1) | ZA202005149B (https=) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20210069628A (ko) * | 2018-10-02 | 2021-06-11 | 도레이 카부시키가이샤 | 헤어핀형 단일쇄 rna 분자의 제조 방법 |
| US20230055682A1 (en) * | 2019-12-03 | 2023-02-23 | Beam Therapeutics lnc. | Synthetic guide rna, compositions, methods, and uses thereof |
| JP7613130B2 (ja) * | 2020-03-25 | 2025-01-15 | 味の素株式会社 | リガーゼ変異体 |
| EP4130018A4 (en) * | 2020-03-27 | 2024-04-17 | Sumitomo Chemical Company, Limited | METHOD FOR PRODUCING A NUCLEIC ACID OLIGOMER |
| EP4392560B1 (en) * | 2021-08-27 | 2026-03-11 | Peking University | Constructs and methods for preparing circular rna |
| CN117660374A (zh) * | 2022-09-08 | 2024-03-08 | 凯莱英医药集团（天津）股份有限公司 | Rna连接酶在寡核苷酸制备上的应用 |
| CN115774075B (zh) * | 2023-02-15 | 2023-06-06 | 江苏耀海生物制药有限公司 | 一种基于RP-HPLC分析体外转录产物成分circRNA的方法 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001040516A2 (en) | 1999-12-02 | 2001-06-07 | Molecular Staging Inc. | Generation of single-strand circular dna from linear self-annealing segments |
| WO2004015075A2 (en) | 2002-08-08 | 2004-02-19 | Dharmacon, Inc. | Short interfering rnas having a hairpin structure containing a non-nucleotide loop |
| JP5296328B2 (ja) | 2007-05-09 | 2013-09-25 | 独立行政法人理化学研究所 | １本鎖環状ｒｎａおよびその製造方法 |
| US20110055965A1 (en) * | 2008-02-15 | 2011-03-03 | Hiroshi Abe | Cycle single-stranded nucleic acid complex and method for producing the same |
| JPWO2011052013A1 (ja) | 2009-10-29 | 2013-03-14 | 株式会社バイオダイナミクス研究所 | 核酸鎖の結合および修飾方法 |
| EP2933333B1 (en) | 2010-07-08 | 2018-02-21 | Bonac Corporation | Single-strand nucleic acid molecule for controlling gene expression |
| EP2639307B1 (en) | 2010-08-03 | 2015-03-04 | Bonac Corporation | Single-stranded nucleic acid molecule having nitrogen-containing alicyclic skeleton |
| MX370398B (es) | 2011-08-25 | 2019-12-11 | Bonac Corp | Compuesto de glicosido, metodo para producir tioeter, eter, metodo para producir eter, metodo para producir un compuesto de glicosido, metodo para producir acido nucleico. |
| WO2013077446A1 (ja) | 2011-11-26 | 2013-05-30 | 株式会社ボナック | 遺伝子発現制御のための一本鎖核酸分子 |
| CN104039961A (zh) | 2012-01-07 | 2014-09-10 | 株式会社博纳克 | 具有氨基酸骨架的单链核酸分子 |
| WO2014110272A1 (en) | 2013-01-09 | 2014-07-17 | The Penn State Research Foundation | Low sequence bias single-stranded dna ligation |
| US10377788B2 (en) | 2015-04-02 | 2019-08-13 | Bonac Corporation | Method for producing glycoside compounds |
| WO2017035090A1 (en) * | 2015-08-21 | 2017-03-02 | Thomas Jefferson University | Dumbbell-pcr: a method to quantify specific small rna variants with a single nucleotide resolution at terminal sequences |
| SG11201803410RA (en) * | 2015-10-30 | 2018-05-30 | Bonac Corp | COMPOSITION STABLY CONTAINING SINGLE-STRANDED NUCLEIC ACID MOLECULE THAT SUPPRESSES EXPRESSION OF TGF-ß1 GENE |
| GB201612011D0 (en) | 2016-07-11 | 2016-08-24 | Glaxosmithkline Ip Dev Ltd | Novel processes for the production of oligonucleotides |
| JP6815601B2 (ja) | 2016-11-01 | 2021-01-20 | スタンレー電気株式会社 | ウルツ鉱構造のＺｎＯＳ混晶粒子の製造方法 |
| JPWO2018182008A1 (ja) | 2017-03-31 | 2020-02-06 | 株式会社ボナック | 遺伝子発現制御機能を有する環状型核酸分子 |
| TWI830718B (zh) | 2018-02-09 | 2024-02-01 | 日商住友化學股份有限公司 | 核酸分子之製造方法 |
| JP6817493B2 (ja) * | 2018-03-30 | 2021-01-20 | 住友化学株式会社 | 一本鎖ｒｎａの製造方法 |
-
2019
- 2019-03-29 US US16/981,329 patent/US11920131B2/en active Active
- 2019-03-29 JP JP2019520762A patent/JP6631751B1/ja not_active Expired - Fee Related
- 2019-03-29 TW TW108111164A patent/TWI772632B/zh active
- 2019-03-29 MX MX2020009556A patent/MX2020009556A/es unknown
- 2019-03-29 AU AU2019242331A patent/AU2019242331A1/en not_active Abandoned
- 2019-03-29 RU RU2020130258A patent/RU2020130258A/ru unknown
- 2019-03-29 CA CA3094160A patent/CA3094160A1/en active Pending
- 2019-03-29 KR KR1020207018860A patent/KR20200136363A/ko active Pending
- 2019-03-29 CN CN201980019534.XA patent/CN111819280A/zh active Pending
- 2019-03-29 BR BR112020017769-5A patent/BR112020017769A2/pt unknown
- 2019-03-29 EP EP19777814.5A patent/EP3778886A4/en not_active Withdrawn
- 2019-03-29 WO PCT/JP2019/013923 patent/WO2019189722A1/ja not_active Ceased
- 2019-10-01 JP JP2019181613A patent/JP7363316B2/ja active Active
-
2020
- 2020-08-19 ZA ZA2020/05149A patent/ZA202005149B/en unknown
- 2020-08-31 SA SA520420072A patent/SA520420072B1/ar unknown
- 2020-09-14 IL IL277346A patent/IL277346A/en unknown
- 2020-09-16 PH PH12020551487A patent/PH12020551487A1/en unknown
-
2024
- 2024-01-26 US US18/424,639 patent/US20240167033A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| MX2020009556A (es) | 2020-10-05 |
| EP3778886A1 (en) | 2021-02-17 |
| JP6631751B1 (ja) | 2020-01-15 |
| AU2019242331A1 (en) | 2020-09-10 |
| EP3778886A4 (en) | 2022-11-02 |
| WO2019189722A1 (ja) | 2019-10-03 |
| TWI772632B (zh) | 2022-08-01 |
| US20210024930A1 (en) | 2021-01-28 |
| JP7363316B2 (ja) | 2023-10-18 |
| CN111819280A (zh) | 2020-10-23 |
| IL277346A (en) | 2020-10-29 |
| JP2019213567A (ja) | 2019-12-19 |
| BR112020017769A2 (pt) | 2021-01-05 |
| RU2020130258A (ru) | 2022-05-04 |
| PH12020551487A1 (en) | 2021-08-23 |
| CA3094160A1 (en) | 2019-10-03 |
| ZA202005149B (en) | 2022-01-26 |
| JPWO2019189722A1 (ja) | 2020-04-30 |
| KR20200136363A (ko) | 2020-12-07 |
| US11920131B2 (en) | 2024-03-05 |
| TW202003842A (zh) | 2020-01-16 |
| US20240167033A1 (en) | 2024-05-23 |
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