CN102057039A - 核酸的制备 - Google Patents

核酸的制备 Download PDF

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Publication number
CN102057039A
CN102057039A CN200980121472XA CN200980121472A CN102057039A CN 102057039 A CN102057039 A CN 102057039A CN 200980121472X A CN200980121472X A CN 200980121472XA CN 200980121472 A CN200980121472 A CN 200980121472A CN 102057039 A CN102057039 A CN 102057039A
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enzyme
mrna
sequence
amino acid
target protein
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Chinese (zh)
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阿尔维达斯·亚努拉提斯
雷米吉犹斯·斯卡尔加拉
唐吉拉·斯克斯内内
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Fermentus Uab
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Fermentus Uab
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Priority to CN201611027264.1A priority Critical patent/CN107058258A/zh
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/162—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN200980121472XA 2008-04-10 2009-04-09 核酸的制备 Pending CN102057039A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201611027264.1A CN107058258A (zh) 2008-04-10 2009-04-09 一种逆转录酶和编码其的多核苷酸

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0806562.5A GB0806562D0 (en) 2008-04-10 2008-04-10 Production of nucleic acid
GB0806562.5 2008-04-10
PCT/EP2009/054329 WO2009125006A2 (en) 2008-04-10 2009-04-09 Production of nucleic acid

Related Child Applications (1)

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CN201611027264.1A Division CN107058258A (zh) 2008-04-10 2009-04-09 一种逆转录酶和编码其的多核苷酸

Publications (1)

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CN102057039A true CN102057039A (zh) 2011-05-11

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CN200980121472XA Pending CN102057039A (zh) 2008-04-10 2009-04-09 核酸的制备
CN201611027264.1A Pending CN107058258A (zh) 2008-04-10 2009-04-09 一种逆转录酶和编码其的多核苷酸

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US (5) US8835148B2 (https=)
EP (4) EP2639300B1 (https=)
JP (5) JP5642662B2 (https=)
KR (4) KR102082768B1 (https=)
CN (2) CN102057039A (https=)
AU (1) AU2009235368B2 (https=)
CA (1) CA2721117A1 (https=)
ES (1) ES2647272T3 (https=)
GB (1) GB0806562D0 (https=)
IL (2) IL208578A (https=)
NZ (1) NZ588468A (https=)
PL (1) PL2281035T3 (https=)
SG (1) SG10201503327QA (https=)
WO (1) WO2009125006A2 (https=)

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CN104250642A (zh) * 2014-04-30 2014-12-31 厦门安普利生物工程有限公司 一种提取纯化m-mlv逆转录酶的方法
CN110709513A (zh) * 2017-04-11 2020-01-17 豪夫迈·罗氏有限公司 具有热稳定性增加的突变型逆转录酶以及涉及其的产物、方法和用途
WO2020132966A1 (zh) * 2018-12-26 2020-07-02 深圳华大生命科学研究院 酶活性提高的逆转录酶及其应用
CN111647576A (zh) * 2020-06-24 2020-09-11 南京诺唯赞生物科技股份有限公司 一种热稳定性逆转录酶突变体及其应用
CN112481234A (zh) * 2020-12-03 2021-03-12 重庆工业职业技术学院 一种高效的焦磷酸酶突变体合成核酸糖类似物及其应用
CN112795546A (zh) * 2019-11-13 2021-05-14 中山大学达安基因股份有限公司 一种具有高逆转录效率的耐高温逆转录酶突变体及其应用
WO2021093845A1 (zh) * 2019-11-13 2021-05-20 中山大学达安基因股份有限公司 耐高温逆转录酶突变体及其应用
CN114480330A (zh) * 2020-11-13 2022-05-13 广州达安基因股份有限公司 多突变位点的逆转录酶突变体
CN114480332A (zh) * 2020-11-13 2022-05-13 广州达安基因股份有限公司 耐高温m-mlv酶突变体及其应用
CN114480335A (zh) * 2020-11-13 2022-05-13 广州达安基因股份有限公司 逆转录酶及逆转录检测试剂
CN114729365A (zh) * 2019-03-19 2022-07-08 布罗德研究所股份有限公司 用于编辑核苷酸序列的方法和组合物
CN115747186A (zh) * 2022-09-13 2023-03-07 天罗诊断科技江苏有限公司 一种耐热的高效率逆转录酶突变体及其应用
CN116732141A (zh) * 2023-07-10 2023-09-12 海南大学 一种快速检测生物dna特异性的方法
CN117925558A (zh) * 2023-12-13 2024-04-26 郑州玛特瑞斯生物科技有限公司 突变型逆转录酶及其应用
CN118406670A (zh) * 2024-05-06 2024-07-30 湖南艾科瑞生物工程有限公司 一种mmlv逆转录酶突变体及其应用
US12281338B2 (en) 2018-10-29 2025-04-22 The Broad Institute, Inc. Nucleobase editors comprising GeoCas9 and uses thereof
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US12359218B2 (en) 2017-07-28 2025-07-15 President And Fellows Of Harvard College Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE)
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US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
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