CN113785053A - 酶活性提高的逆转录酶及其应用 - Google Patents

酶活性提高的逆转录酶及其应用 Download PDF

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CN113785053A
CN113785053A CN201880100449.1A CN201880100449A CN113785053A CN 113785053 A CN113785053 A CN 113785053A CN 201880100449 A CN201880100449 A CN 201880100449A CN 113785053 A CN113785053 A CN 113785053A
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reverse transcriptase
nucleic acid
activity
mlv
acid molecule
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刘欢欢
郭娜
李慧真
张周刚
韩鸿雁
郭苗苗
郑越
董宇亮
章文蔚
徐崇钧
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BGI Shenzhen Co Ltd
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BGI Shenzhen Co Ltd
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Abstract

本发明涉及一种逆转录酶及其应用。该逆转录酶与野生型的M‑MLV逆转录酶相比，具有R450H等突变位点。该逆转录酶具有提高的聚合酶活性、提高的热稳定性和降低的RNase H活性。

Description

PCT国内申请，说明书已公开。

Claims (30)

  1. PCT国内申请，权利要求书已公开。
CN201880100449.1A 2018-12-26 2018-12-26 酶活性提高的逆转录酶及其应用 Pending CN113785053A (zh)

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PCT/CN2018/123994 WO2020132966A1 (zh) 2018-12-26 2018-12-26 酶活性提高的逆转录酶及其应用

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024092713A1 (zh) * 2022-11-04 2024-05-10 深圳华大生命科学研究院 Mmlv逆转录酶突变体

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114480329B (zh) * 2020-11-13 2023-06-30 广州达安基因股份有限公司 高效率mmlv酶突变体
CN114480337B (zh) * 2020-11-13 2023-07-28 广州达安基因股份有限公司 逆转录酶突变体及逆转录方法
CN116926039A (zh) * 2023-09-19 2023-10-24 魔因生物科技(北京)有限公司 反转录酶HIV p66突变体及其应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1259957A (zh) * 1997-04-22 2000-07-12 生命技术公司 产生由多个亚单位组成的aslv逆转录酶的方法
EP1931772A2 (en) * 2005-08-10 2008-06-18 Stratagene California Mutant reverse transcriptase and methods of use
CN102057039A (zh) * 2008-04-10 2011-05-11 菲门特斯Uab公司 核酸的制备
CN106164261A (zh) * 2014-01-22 2016-11-23 生命技术公司 适用于高温核酸合成的新颖逆转录酶
CN106906237A (zh) * 2017-04-18 2017-06-30 淮海工学院 一种高性能m‑mlv逆转录酶的制备方法

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1259957A (zh) * 1997-04-22 2000-07-12 生命技术公司 产生由多个亚单位组成的aslv逆转录酶的方法
EP1931772A2 (en) * 2005-08-10 2008-06-18 Stratagene California Mutant reverse transcriptase and methods of use
CN102057039A (zh) * 2008-04-10 2011-05-11 菲门特斯Uab公司 核酸的制备
CN106164261A (zh) * 2014-01-22 2016-11-23 生命技术公司 适用于高温核酸合成的新颖逆转录酶
US20180010105A1 (en) * 2014-01-22 2018-01-11 Life Technologies Corporation Novel reverse transcriptases for use in high temperature nucleic acid synthesis
CN106906237A (zh) * 2017-04-18 2017-06-30 淮海工学院 一种高性能m‑mlv逆转录酶的制备方法

Non-Patent Citations (1)

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Title
MAR ÁLVAREZ等: "Increased Thermostability and Fidelity of DNA Synthesis of Wild-Type and Mutant HIV-1 Group O Reverse Transcriptases", 《J. MOL. BIOL.》, vol. 392, no. 4, pages 872 - 884, XP055295571, DOI: 10.1016/j.jmb.2009.07.081 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024092713A1 (zh) * 2022-11-04 2024-05-10 深圳华大生命科学研究院 Mmlv逆转录酶突变体

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