IL208578A - Enzyme Revers - transcriptase including mutant murine murine leukemia virus and polynucleotide encoding it - Google Patents

Enzyme Revers - transcriptase including mutant murine murine leukemia virus and polynucleotide encoding it

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Publication number
IL208578A
IL208578A IL208578A IL20857810A IL208578A IL 208578 A IL208578 A IL 208578A IL 208578 A IL208578 A IL 208578A IL 20857810 A IL20857810 A IL 20857810A IL 208578 A IL208578 A IL 208578A
Authority
IL
Israel
Prior art keywords
polynucleotide encoding
reverse transcriptase
leukemia virus
murine leukemia
moloney murine
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IL208578A
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English (en)
Hebrew (he)
Other versions
IL208578A0 (en
Original Assignee
Thermo Fisher Scientific Baltics Uab
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Publication date
Application filed by Thermo Fisher Scientific Baltics Uab filed Critical Thermo Fisher Scientific Baltics Uab
Publication of IL208578A0 publication Critical patent/IL208578A0/en
Publication of IL208578A publication Critical patent/IL208578A/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/162Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from virus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1075Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1276RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07Nucleotidyltransferases (2.7.7)
    • C12Y207/07049RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
IL208578A 2008-04-10 2010-10-07 Enzyme Revers - transcriptase including mutant murine murine leukemia virus and polynucleotide encoding it IL208578A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0806562.5A GB0806562D0 (en) 2008-04-10 2008-04-10 Production of nucleic acid
PCT/EP2009/054329 WO2009125006A2 (en) 2008-04-10 2009-04-09 Production of nucleic acid

Publications (2)

Publication Number Publication Date
IL208578A0 IL208578A0 (en) 2010-12-30
IL208578A true IL208578A (en) 2016-07-31

Family

ID=39433452

Family Applications (2)

Application Number Title Priority Date Filing Date
IL208578A IL208578A (en) 2008-04-10 2010-10-07 Enzyme Revers - transcriptase including mutant murine murine leukemia virus and polynucleotide encoding it
IL246396A IL246396B (en) 2008-04-10 2016-06-22 A method for producing a nucleic acid encoding a target protein with enzymatic activity

Family Applications After (1)

Application Number Title Priority Date Filing Date
IL246396A IL246396B (en) 2008-04-10 2016-06-22 A method for producing a nucleic acid encoding a target protein with enzymatic activity

Country Status (14)

Country Link
US (5) US8835148B2 (https=)
EP (4) EP2639300B1 (https=)
JP (5) JP5642662B2 (https=)
KR (4) KR102082768B1 (https=)
CN (2) CN102057039A (https=)
AU (1) AU2009235368B2 (https=)
CA (1) CA2721117A1 (https=)
ES (1) ES2647272T3 (https=)
GB (1) GB0806562D0 (https=)
IL (2) IL208578A (https=)
NZ (1) NZ588468A (https=)
PL (1) PL2281035T3 (https=)
SG (1) SG10201503327QA (https=)
WO (1) WO2009125006A2 (https=)

Families Citing this family (100)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9771565B2 (en) * 2000-05-26 2017-09-26 Life Technologies Corporation Thermostable reverse transcriptases and uses thereof
WO2004024749A2 (en) 2002-09-13 2004-03-25 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
WO2008151023A2 (en) 2007-06-01 2008-12-11 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
GB0806562D0 (en) * 2008-04-10 2008-05-14 Fermentas Uab Production of nucleic acid
EP2488656B1 (en) * 2009-10-15 2015-06-03 Ibis Biosciences, Inc. Multiple displacement amplification
US8900814B2 (en) 2010-08-13 2014-12-02 Kyoto University Variant reverse transcriptase
KR101818126B1 (ko) 2011-02-09 2018-01-15 (주)바이오니아 열안정성이 증가된 역전사효소
US10323236B2 (en) 2011-07-22 2019-06-18 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
US9745571B2 (en) * 2013-03-07 2017-08-29 Bio-Rad Laboratories, Inc. Repetitive reverse transcription partition assay
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
WO2015075842A1 (ja) * 2013-11-25 2015-05-28 国立大学法人京都大学 変異型逆転写酵素
US20150165054A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting caspase-9 point mutations
JP2017503521A (ja) * 2014-01-22 2017-02-02 ライフ テクノロジーズ コーポレーション 高温核酸合成で使用するための新規な逆転写酵素
US10710081B2 (en) 2014-03-14 2020-07-14 Life Technologies Corporation Integrated system for nucleic acid amplification and detection
CN104250642A (zh) * 2014-04-30 2014-12-31 厦门安普利生物工程有限公司 一种提取纯化m-mlv逆转录酶的方法
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
RU2724998C2 (ru) 2015-05-11 2020-06-29 Иллюмина, Инк. Платформа для обнаружения и анализа терапевтических агентов
IL310721B2 (en) 2015-10-23 2025-11-01 Harvard College Nucleobase editors and uses thereof
US11046940B2 (en) 2016-04-12 2021-06-29 Solis Biodyne Ou Synthetic reverse transcriptases and uses thereof
CN110214183A (zh) 2016-08-03 2019-09-06 哈佛大学的校长及成员们 腺苷核碱基编辑器及其用途
WO2018031683A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
US9580698B1 (en) * 2016-09-23 2017-02-28 New England Biolabs, Inc. Mutant reverse transcriptase
KR102622411B1 (ko) 2016-10-14 2024-01-10 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
US11220677B2 (en) 2016-12-14 2022-01-11 Takara Bio Inc. Heat-resistant reverse transcriptase mutant
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
US12390514B2 (en) 2017-03-09 2025-08-19 President And Fellows Of Harvard College Cancer vaccine
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
US11542496B2 (en) 2017-03-10 2023-01-03 President And Fellows Of Harvard College Cytosine to guanine base editor
KR20240116572A (ko) 2017-03-23 2024-07-29 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제
CN110709513B (zh) * 2017-04-11 2023-10-03 豪夫迈·罗氏有限公司 具有热稳定性增加的突变型逆转录酶以及涉及其的产物、方法和用途
WO2018200867A1 (en) * 2017-04-26 2018-11-01 10X Genomics, Inc. Mmlv reverse transcriptase variants
US20180312822A1 (en) 2017-04-26 2018-11-01 10X Genomics, Inc. Mmlv reverse transcriptase variants
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
US11649442B2 (en) 2017-09-08 2023-05-16 The Regents Of The University Of California RNA-guided endonuclease fusion polypeptides and methods of use thereof
KR20250107288A (ko) 2017-10-16 2025-07-11 더 브로드 인스티튜트, 인코퍼레이티드 아데노신 염기 편집제의 용도
US12406749B2 (en) 2017-12-15 2025-09-02 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
US20200392562A1 (en) 2017-12-22 2020-12-17 Thermo Fisher Scientific Baltics Uab Polymerase chain reaction composition comprising amines
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
US12522807B2 (en) 2018-07-09 2026-01-13 The Broad Institute, Inc. RNA programmable epigenetic RNA modifiers and uses thereof
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20220411783A1 (en) * 2018-10-12 2022-12-29 The Broad Institute, Inc. Method for extracting nuclei or whole cells from formalin-fixed paraffin-embedded tissues
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
WO2020093322A1 (zh) * 2018-11-08 2020-05-14 深圳华大生命科学研究院 一种微流控芯片及其制备方法和dna合成方法
CN113785053B (zh) * 2018-12-26 2024-07-12 深圳华大生命科学研究院 酶活性提高的逆转录酶及其应用
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
WO2020191233A1 (en) 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
JP7593316B2 (ja) * 2019-07-26 2024-12-03 東洋紡株式会社 安定性に優れた変異型逆転写酵素
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
WO2021072306A1 (en) 2019-10-10 2021-04-15 1859, Inc. Methods and systems for microfluidic screening
CN112795549B (zh) * 2019-11-13 2023-11-28 广州达安基因股份有限公司 一种逆转录酶突变体
CN112795548A (zh) * 2019-11-13 2021-05-14 中山大学达安基因股份有限公司 具有高逆转录效率的耐高温逆转录酶突变体及其应用
CN112795546B (zh) * 2019-11-13 2024-02-27 广州达安基因股份有限公司 一种具有高逆转录效率的耐高温逆转录酶突变体及其应用
CN112795551B (zh) * 2019-11-13 2024-01-30 广州达安基因股份有限公司 一种耐高温逆转录酶突变体及其应用
CN112795547B (zh) * 2019-11-13 2024-05-31 广州达安基因股份有限公司 高逆转录效率的逆转录酶突变体
EP4073241A2 (en) 2019-12-11 2022-10-19 10X Genomics, Inc. Reverse transcriptase variants
WO2021165508A1 (en) 2020-02-21 2021-08-26 Biogemma Prime editing technology for plant genome engineering
JP2023518395A (ja) 2020-03-19 2023-05-01 インテリア セラピューティクス,インコーポレイテッド 指向性ゲノム編集のための方法及び組成物
IL297761A (en) 2020-05-08 2022-12-01 Broad Inst Inc Methods and compositions for simultaneously editing two helices of a designated double-helix nucleotide sequence
CN111849938B (zh) * 2020-06-05 2022-02-15 广州英赞生物科技有限公司 一种突变型逆转录酶及其制备方法与应用
CN111647576B (zh) * 2020-06-24 2021-02-09 南京诺唯赞生物科技股份有限公司 一种热稳定性逆转录酶突变体及其应用
US12435318B2 (en) 2020-07-20 2025-10-07 Integrated Dna Technologies, Inc. Reverse transcriptase mutants with increased activity and thermostability
US12559734B2 (en) 2020-07-20 2026-02-24 Integrated Dna Technologies, Inc. Reverse transcriptase mutants with increased activity and thermostability
CN114480332B (zh) * 2020-11-13 2023-12-05 广州达安基因股份有限公司 耐高温m-mlv酶突变体及其应用
CN114480336B (zh) * 2020-11-13 2023-10-20 广州达安基因股份有限公司 含有逆转录酶突变体的核酸检测试剂盒
CN114480330B (zh) * 2020-11-13 2024-11-01 广州达安基因股份有限公司 多突变位点的逆转录酶突变体
CN114480331A (zh) * 2020-11-13 2022-05-13 广州达安基因股份有限公司 Mmlv酶组合突变体
CN114480335B (zh) * 2020-11-13 2024-06-07 广州达安基因股份有限公司 逆转录酶及逆转录检测试剂
WO2022115579A1 (en) * 2020-11-24 2022-06-02 Saliogen Therapeutics, Inc. Mammalian mobile element compositions, systems and therapeutic applications
CN112481234B (zh) * 2020-12-03 2023-09-22 重庆工业职业技术学院 一种焦磷酸酶突变体合成核酸糖类似物及其应用
CN112251423B (zh) * 2020-12-08 2021-03-19 北京健为医学检验实验室有限公司 一种m-mlv逆转录酶体及其应用
CN113234858A (zh) * 2021-05-20 2021-08-10 阿吉安(福州)基因医学检验实验室有限公司 一种covid-19的检测试剂盒
AU2022282559A1 (en) * 2021-05-26 2024-01-04 Etherna Immunotherapies Nv Method to reduce double stranded rna by-product formation
CN113174381B (zh) * 2021-06-08 2023-05-12 翌圣生物科技(上海)股份有限公司 增强型mmlv逆转录酶突变体及其应用
CN118401656A (zh) * 2021-12-17 2024-07-26 东洋纺株式会社 热稳定性优异的逆转录酶
CN114214299A (zh) * 2022-01-26 2022-03-22 苏州译酶生物科技有限公司 一种m-mlv逆转录酶及其制备方法
US11639499B1 (en) 2022-03-08 2023-05-02 Watchmaker Genomics, Inc. Reverse transcriptase variants
CN114657165B (zh) * 2022-03-25 2023-04-07 江苏百时美生物科技有限公司 一种提高热稳定性和抑制剂耐受性的逆转录酶突变体及其应用
CN114591930B (zh) * 2022-05-10 2022-08-12 翌圣生物科技(上海)股份有限公司 增强型逆转录酶、编码dna、试剂盒及其在rna文库构建中的应用
CN117106745B (zh) * 2022-06-01 2025-02-14 北京擎科生物科技股份有限公司 逆转录突变体及其应用
CN116064460B (zh) * 2022-08-01 2023-09-26 南京诺唯赞生物科技股份有限公司 一种mmlv突变体及其应用
CN117586983A (zh) * 2022-08-11 2024-02-23 菲鹏生物股份有限公司 一种逆转录酶、核酸分子及合成cDNA的方法
CN115747186B (zh) * 2022-09-13 2025-05-27 天罗诊断科技江苏有限公司 一种耐热的高效率逆转录酶突变体及其应用
CN116042569B (zh) * 2023-02-01 2023-08-15 珠海宝锐生物科技有限公司 Mmlv逆转录酶突变体及其应用
CN116004568B (zh) * 2023-02-07 2025-10-17 北京诺派生物科技有限公司 一种可用于核酸检测的耐高温反转录酶及其制备方法与应用
EP4689163A1 (en) * 2023-04-05 2026-02-11 Primrose Bio, Inc. Methods and compositions for protein engineering
CN116622669B (zh) * 2023-06-30 2024-06-07 翌圣生物科技(上海)股份有限公司 一种鼠白血病病毒逆转录酶突变体及其应用
CN116694598B (zh) * 2023-06-30 2024-12-13 翌圣生物科技(上海)股份有限公司 一种鼠白血病病毒逆转录酶突变体及其应用
CN116732141B (zh) * 2023-07-10 2024-04-02 海南大学 一种快速检测生物dna特异性的方法
CN117025724A (zh) * 2023-08-23 2023-11-10 翌圣生物科技(上海)股份有限公司 Rna链特异性建库试剂盒及建库方法
CN117925558B (zh) * 2023-12-13 2025-03-25 郑州玛特瑞斯生物科技有限公司 突变型逆转录酶及其应用
CN118406670B (zh) * 2024-05-06 2025-06-27 湖南艾科瑞生物工程有限公司 一种mmlv逆转录酶突变体及其应用
WO2026003215A1 (fr) 2024-06-27 2026-01-02 Commissariat A L'energie Atomique Et Aux Energies Alternatives Bases modifiées chimiquement avec un groupement clivable et méthodes de sélection d'acides nucléiques fonctionnels les comprenant
CN119955759B (zh) * 2025-01-07 2025-08-08 湖南艾科瑞生物工程有限公司 一种mmlv逆转录酶突变体及其应用
CN121249621B (zh) * 2025-12-08 2026-03-31 翌圣生物科技(上海)股份有限公司 一种mmlv逆转录酶突变体及其制备方法、产品和应用

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6236563A (ja) 1985-08-12 1987-02-17 Koji Ando 可動導体型計器
US5244797B1 (en) * 1988-01-13 1998-08-25 Life Technologies Inc Cloned genes encoding reverse transcriptase lacking rnase h activity
AU638762B2 (en) 1989-10-05 1993-07-08 Optein Inc Cell-free synthesis and isolation of novel genes and polypeptides
US6183967B1 (en) * 1995-06-07 2001-02-06 Nexstar Pharmaceuticals Nucleic acid ligand inhibitors to DNA polymerases
WO1998023733A2 (en) 1996-11-27 1998-06-04 University Of Washington Thermostable polymerases having altered fidelity
US6989259B2 (en) 1997-04-22 2006-01-24 Invitrogen Corporation Recombinant methods for making reverse transcriptases and mutants thereof
ES2230701T3 (es) 1997-07-07 2005-05-01 Medical Research Council Procedimiento de seleccion in vitro.
US6136582A (en) * 1998-01-20 2000-10-24 The Trustees Of Columbia University In The City Reverse transcriptase of moloney murine leukemia virus with RNA polymerase activity
JP4193079B2 (ja) 1998-11-10 2008-12-10 東洋紡績株式会社 変異型逆転写酵素
EP1149170A1 (en) * 1999-01-15 2001-10-31 Molecular Biology Resources Biologically active reverse transcriptases
AU4741301A (en) 2000-03-15 2001-09-24 Invitrogen Corp High fidelity reverse transcriptases and uses thereof
CA2402452A1 (en) 2000-03-31 2001-10-11 Cambridge Antibody Technology Limited Improvements to ribosome display
US7078208B2 (en) 2000-05-26 2006-07-18 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
CN1430670A (zh) * 2000-05-26 2003-07-16 茵维特罗根公司 热稳定逆转录酶及其用途
GB0022458D0 (en) 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
US20070020622A1 (en) 2001-09-14 2007-01-25 Invitrogen Corporation DNA Polymerases and mutants thereof
GB0127564D0 (en) * 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
GB2388031B (en) 2002-04-30 2005-07-27 Abbott Lab A lancet removal tool
DK1534830T3 (da) * 2002-09-06 2011-02-14 Isogenica Ltd In vito-peptid-ekspressionsbibliotek
GB0221053D0 (en) 2002-09-11 2002-10-23 Medical Res Council Single-molecule in vitro evolution
WO2004024749A2 (en) * 2002-09-13 2004-03-25 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
EP1691792A4 (en) * 2003-11-24 2008-05-28 Yeda Res & Dev COMPOSITIONS AND PROCESSES FOR SORTING IN VITRO OF MOLECULE AND CELL LIBRARIES
WO2005071072A1 (en) 2004-01-13 2005-08-04 The Institute Of Biophysics Chinese Academy Of Sciences The recombinant murine leukemia virus reverse transcriptase, the gene encoding it and the method for expressing it
US7595179B2 (en) * 2004-04-19 2009-09-29 Applied Biosystems, Llc Recombinant reverse transcriptases
US20080004436A1 (en) 2004-11-15 2008-01-03 Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science Directed Evolution and Selection Using in Vitro Compartmentalization
JPWO2007011045A1 (ja) * 2005-07-20 2009-02-05 株式会社クラレ パージング剤
WO2007022045A2 (en) * 2005-08-10 2007-02-22 Stratagene California Mutant reverse transcriptase and methods of use
ES2404665T3 (es) 2006-09-06 2013-05-28 Medical Research Council Polimerasas de ADN que incorporan análogos de nucleótidos marcados con colorante
GB0806562D0 (en) * 2008-04-10 2008-05-14 Fermentas Uab Production of nucleic acid

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