KR102082768B1 - 핵산 제조 방법 - Google Patents

핵산 제조 방법 Download PDF

Info

Publication number
KR102082768B1
KR102082768B1 KR1020197003483A KR20197003483A KR102082768B1 KR 102082768 B1 KR102082768 B1 KR 102082768B1 KR 1020197003483 A KR1020197003483 A KR 1020197003483A KR 20197003483 A KR20197003483 A KR 20197003483A KR 102082768 B1 KR102082768 B1 KR 102082768B1
Authority
KR
South Korea
Prior art keywords
leu
ala
pro
thr
gly
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020197003483A
Other languages
English (en)
Korean (ko)
Other versions
KR20190016132A (ko
Inventor
아르바이다스 자누라이티스
레미지 주스 스키르가일라
단기라 식스니에네
Original Assignee
써모 피셔 사이언티픽 발틱스 유에이비
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 써모 피셔 사이언티픽 발틱스 유에이비 filed Critical 써모 피셔 사이언티픽 발틱스 유에이비
Publication of KR20190016132A publication Critical patent/KR20190016132A/ko
Application granted granted Critical
Publication of KR102082768B1 publication Critical patent/KR102082768B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/162—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020197003483A 2008-04-10 2009-04-09 핵산 제조 방법 Active KR102082768B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0806562.5A GB0806562D0 (en) 2008-04-10 2008-04-10 Production of nucleic acid
GB0806562.5 2008-04-10
PCT/EP2009/054329 WO2009125006A2 (en) 2008-04-10 2009-04-09 Production of nucleic acid

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR1020187003649A Division KR101947101B1 (ko) 2008-04-10 2009-04-09 핵산 제조 방법

Publications (2)

Publication Number Publication Date
KR20190016132A KR20190016132A (ko) 2019-02-15
KR102082768B1 true KR102082768B1 (ko) 2020-02-28

Family

ID=39433452

Family Applications (4)

Application Number Title Priority Date Filing Date
KR1020197003483A Active KR102082768B1 (ko) 2008-04-10 2009-04-09 핵산 제조 방법
KR1020187003649A Active KR101947101B1 (ko) 2008-04-10 2009-04-09 핵산 제조 방법
KR1020167013385A Active KR101828902B1 (ko) 2008-04-10 2009-04-09 핵산 제조 방법
KR1020107025153A Active KR101653775B1 (ko) 2008-04-10 2009-04-09 핵산 제조 방법

Family Applications After (3)

Application Number Title Priority Date Filing Date
KR1020187003649A Active KR101947101B1 (ko) 2008-04-10 2009-04-09 핵산 제조 방법
KR1020167013385A Active KR101828902B1 (ko) 2008-04-10 2009-04-09 핵산 제조 방법
KR1020107025153A Active KR101653775B1 (ko) 2008-04-10 2009-04-09 핵산 제조 방법

Country Status (14)

Country Link
US (5) US8835148B2 (https=)
EP (4) EP2639300B1 (https=)
JP (5) JP5642662B2 (https=)
KR (4) KR102082768B1 (https=)
CN (2) CN102057039A (https=)
AU (1) AU2009235368B2 (https=)
CA (1) CA2721117A1 (https=)
ES (1) ES2647272T3 (https=)
GB (1) GB0806562D0 (https=)
IL (2) IL208578A (https=)
NZ (1) NZ588468A (https=)
PL (1) PL2281035T3 (https=)
SG (1) SG10201503327QA (https=)
WO (1) WO2009125006A2 (https=)

Families Citing this family (100)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9771565B2 (en) * 2000-05-26 2017-09-26 Life Technologies Corporation Thermostable reverse transcriptases and uses thereof
WO2004024749A2 (en) 2002-09-13 2004-03-25 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
WO2008151023A2 (en) 2007-06-01 2008-12-11 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
GB0806562D0 (en) * 2008-04-10 2008-05-14 Fermentas Uab Production of nucleic acid
EP2488656B1 (en) * 2009-10-15 2015-06-03 Ibis Biosciences, Inc. Multiple displacement amplification
US8900814B2 (en) 2010-08-13 2014-12-02 Kyoto University Variant reverse transcriptase
KR101818126B1 (ko) 2011-02-09 2018-01-15 (주)바이오니아 열안정성이 증가된 역전사효소
US10323236B2 (en) 2011-07-22 2019-06-18 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
US9745571B2 (en) * 2013-03-07 2017-08-29 Bio-Rad Laboratories, Inc. Repetitive reverse transcription partition assay
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
WO2015075842A1 (ja) * 2013-11-25 2015-05-28 国立大学法人京都大学 変異型逆転写酵素
US20150165054A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting caspase-9 point mutations
JP2017503521A (ja) * 2014-01-22 2017-02-02 ライフ テクノロジーズ コーポレーション 高温核酸合成で使用するための新規な逆転写酵素
US10710081B2 (en) 2014-03-14 2020-07-14 Life Technologies Corporation Integrated system for nucleic acid amplification and detection
CN104250642A (zh) * 2014-04-30 2014-12-31 厦门安普利生物工程有限公司 一种提取纯化m-mlv逆转录酶的方法
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
RU2724998C2 (ru) 2015-05-11 2020-06-29 Иллюмина, Инк. Платформа для обнаружения и анализа терапевтических агентов
IL310721B2 (en) 2015-10-23 2025-11-01 Harvard College Nucleobase editors and uses thereof
US11046940B2 (en) 2016-04-12 2021-06-29 Solis Biodyne Ou Synthetic reverse transcriptases and uses thereof
CN110214183A (zh) 2016-08-03 2019-09-06 哈佛大学的校长及成员们 腺苷核碱基编辑器及其用途
WO2018031683A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
US9580698B1 (en) * 2016-09-23 2017-02-28 New England Biolabs, Inc. Mutant reverse transcriptase
KR102622411B1 (ko) 2016-10-14 2024-01-10 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
US11220677B2 (en) 2016-12-14 2022-01-11 Takara Bio Inc. Heat-resistant reverse transcriptase mutant
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
US12390514B2 (en) 2017-03-09 2025-08-19 President And Fellows Of Harvard College Cancer vaccine
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
US11542496B2 (en) 2017-03-10 2023-01-03 President And Fellows Of Harvard College Cytosine to guanine base editor
KR20240116572A (ko) 2017-03-23 2024-07-29 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제
CN110709513B (zh) * 2017-04-11 2023-10-03 豪夫迈·罗氏有限公司 具有热稳定性增加的突变型逆转录酶以及涉及其的产物、方法和用途
WO2018200867A1 (en) * 2017-04-26 2018-11-01 10X Genomics, Inc. Mmlv reverse transcriptase variants
US20180312822A1 (en) 2017-04-26 2018-11-01 10X Genomics, Inc. Mmlv reverse transcriptase variants
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
US11649442B2 (en) 2017-09-08 2023-05-16 The Regents Of The University Of California RNA-guided endonuclease fusion polypeptides and methods of use thereof
KR20250107288A (ko) 2017-10-16 2025-07-11 더 브로드 인스티튜트, 인코퍼레이티드 아데노신 염기 편집제의 용도
US12406749B2 (en) 2017-12-15 2025-09-02 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
US20200392562A1 (en) 2017-12-22 2020-12-17 Thermo Fisher Scientific Baltics Uab Polymerase chain reaction composition comprising amines
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
US12522807B2 (en) 2018-07-09 2026-01-13 The Broad Institute, Inc. RNA programmable epigenetic RNA modifiers and uses thereof
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20220411783A1 (en) * 2018-10-12 2022-12-29 The Broad Institute, Inc. Method for extracting nuclei or whole cells from formalin-fixed paraffin-embedded tissues
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
WO2020093322A1 (zh) * 2018-11-08 2020-05-14 深圳华大生命科学研究院 一种微流控芯片及其制备方法和dna合成方法
CN113785053B (zh) * 2018-12-26 2024-07-12 深圳华大生命科学研究院 酶活性提高的逆转录酶及其应用
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
WO2020191233A1 (en) 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
JP7593316B2 (ja) * 2019-07-26 2024-12-03 東洋紡株式会社 安定性に優れた変異型逆転写酵素
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
WO2021072306A1 (en) 2019-10-10 2021-04-15 1859, Inc. Methods and systems for microfluidic screening
CN112795549B (zh) * 2019-11-13 2023-11-28 广州达安基因股份有限公司 一种逆转录酶突变体
CN112795548A (zh) * 2019-11-13 2021-05-14 中山大学达安基因股份有限公司 具有高逆转录效率的耐高温逆转录酶突变体及其应用
CN112795546B (zh) * 2019-11-13 2024-02-27 广州达安基因股份有限公司 一种具有高逆转录效率的耐高温逆转录酶突变体及其应用
CN112795551B (zh) * 2019-11-13 2024-01-30 广州达安基因股份有限公司 一种耐高温逆转录酶突变体及其应用
CN112795547B (zh) * 2019-11-13 2024-05-31 广州达安基因股份有限公司 高逆转录效率的逆转录酶突变体
EP4073241A2 (en) 2019-12-11 2022-10-19 10X Genomics, Inc. Reverse transcriptase variants
WO2021165508A1 (en) 2020-02-21 2021-08-26 Biogemma Prime editing technology for plant genome engineering
JP2023518395A (ja) 2020-03-19 2023-05-01 インテリア セラピューティクス，インコーポレイテッド 指向性ゲノム編集のための方法及び組成物
IL297761A (en) 2020-05-08 2022-12-01 Broad Inst Inc Methods and compositions for simultaneously editing two helices of a designated double-helix nucleotide sequence
CN111849938B (zh) * 2020-06-05 2022-02-15 广州英赞生物科技有限公司 一种突变型逆转录酶及其制备方法与应用
CN111647576B (zh) * 2020-06-24 2021-02-09 南京诺唯赞生物科技股份有限公司 一种热稳定性逆转录酶突变体及其应用
US12435318B2 (en) 2020-07-20 2025-10-07 Integrated Dna Technologies, Inc. Reverse transcriptase mutants with increased activity and thermostability
US12559734B2 (en) 2020-07-20 2026-02-24 Integrated Dna Technologies, Inc. Reverse transcriptase mutants with increased activity and thermostability
CN114480332B (zh) * 2020-11-13 2023-12-05 广州达安基因股份有限公司 耐高温m-mlv酶突变体及其应用
CN114480336B (zh) * 2020-11-13 2023-10-20 广州达安基因股份有限公司 含有逆转录酶突变体的核酸检测试剂盒
CN114480330B (zh) * 2020-11-13 2024-11-01 广州达安基因股份有限公司 多突变位点的逆转录酶突变体
CN114480331A (zh) * 2020-11-13 2022-05-13 广州达安基因股份有限公司 Mmlv酶组合突变体
CN114480335B (zh) * 2020-11-13 2024-06-07 广州达安基因股份有限公司 逆转录酶及逆转录检测试剂
WO2022115579A1 (en) * 2020-11-24 2022-06-02 Saliogen Therapeutics, Inc. Mammalian mobile element compositions, systems and therapeutic applications
CN112481234B (zh) * 2020-12-03 2023-09-22 重庆工业职业技术学院 一种焦磷酸酶突变体合成核酸糖类似物及其应用
CN112251423B (zh) * 2020-12-08 2021-03-19 北京健为医学检验实验室有限公司 一种m-mlv逆转录酶体及其应用
CN113234858A (zh) * 2021-05-20 2021-08-10 阿吉安(福州)基因医学检验实验室有限公司 一种covid-19的检测试剂盒
AU2022282559A1 (en) * 2021-05-26 2024-01-04 Etherna Immunotherapies Nv Method to reduce double stranded rna by-product formation
CN113174381B (zh) * 2021-06-08 2023-05-12 翌圣生物科技(上海)股份有限公司 增强型mmlv逆转录酶突变体及其应用
CN118401656A (zh) * 2021-12-17 2024-07-26 东洋纺株式会社 热稳定性优异的逆转录酶
CN114214299A (zh) * 2022-01-26 2022-03-22 苏州译酶生物科技有限公司 一种m-mlv逆转录酶及其制备方法
US11639499B1 (en) 2022-03-08 2023-05-02 Watchmaker Genomics, Inc. Reverse transcriptase variants
CN114657165B (zh) * 2022-03-25 2023-04-07 江苏百时美生物科技有限公司 一种提高热稳定性和抑制剂耐受性的逆转录酶突变体及其应用
CN114591930B (zh) * 2022-05-10 2022-08-12 翌圣生物科技(上海)股份有限公司 增强型逆转录酶、编码dna、试剂盒及其在rna文库构建中的应用
CN117106745B (zh) * 2022-06-01 2025-02-14 北京擎科生物科技股份有限公司 逆转录突变体及其应用
CN116064460B (zh) * 2022-08-01 2023-09-26 南京诺唯赞生物科技股份有限公司 一种mmlv突变体及其应用
CN117586983A (zh) * 2022-08-11 2024-02-23 菲鹏生物股份有限公司 一种逆转录酶、核酸分子及合成cDNA的方法
CN115747186B (zh) * 2022-09-13 2025-05-27 天罗诊断科技江苏有限公司 一种耐热的高效率逆转录酶突变体及其应用
CN116042569B (zh) * 2023-02-01 2023-08-15 珠海宝锐生物科技有限公司 Mmlv逆转录酶突变体及其应用
CN116004568B (zh) * 2023-02-07 2025-10-17 北京诺派生物科技有限公司 一种可用于核酸检测的耐高温反转录酶及其制备方法与应用
EP4689163A1 (en) * 2023-04-05 2026-02-11 Primrose Bio, Inc. Methods and compositions for protein engineering
CN116622669B (zh) * 2023-06-30 2024-06-07 翌圣生物科技(上海)股份有限公司 一种鼠白血病病毒逆转录酶突变体及其应用
CN116694598B (zh) * 2023-06-30 2024-12-13 翌圣生物科技(上海)股份有限公司 一种鼠白血病病毒逆转录酶突变体及其应用
CN116732141B (zh) * 2023-07-10 2024-04-02 海南大学 一种快速检测生物dna特异性的方法
CN117025724A (zh) * 2023-08-23 2023-11-10 翌圣生物科技(上海)股份有限公司 Rna链特异性建库试剂盒及建库方法
CN117925558B (zh) * 2023-12-13 2025-03-25 郑州玛特瑞斯生物科技有限公司 突变型逆转录酶及其应用
CN118406670B (zh) * 2024-05-06 2025-06-27 湖南艾科瑞生物工程有限公司 一种mmlv逆转录酶突变体及其应用
WO2026003215A1 (fr) 2024-06-27 2026-01-02 Commissariat A L'energie Atomique Et Aux Energies Alternatives Bases modifiées chimiquement avec un groupement clivable et méthodes de sélection d'acides nucléiques fonctionnels les comprenant
CN119955759B (zh) * 2025-01-07 2025-08-08 湖南艾科瑞生物工程有限公司 一种mmlv逆转录酶突变体及其应用
CN121249621B (zh) * 2025-12-08 2026-03-31 翌圣生物科技(上海)股份有限公司 一种mmlv逆转录酶突变体及其制备方法、产品和应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101653775B1 (ko) * 2008-04-10 2016-09-05 써모 피셔 사이언티픽 발틱스 유에이비 핵산 제조 방법

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6236563A (ja) 1985-08-12 1987-02-17 Koji Ando 可動導体型計器
US5244797B1 (en) * 1988-01-13 1998-08-25 Life Technologies Inc Cloned genes encoding reverse transcriptase lacking rnase h activity
AU638762B2 (en) 1989-10-05 1993-07-08 Optein Inc Cell-free synthesis and isolation of novel genes and polypeptides
US6183967B1 (en) * 1995-06-07 2001-02-06 Nexstar Pharmaceuticals Nucleic acid ligand inhibitors to DNA polymerases
WO1998023733A2 (en) 1996-11-27 1998-06-04 University Of Washington Thermostable polymerases having altered fidelity
US6989259B2 (en) 1997-04-22 2006-01-24 Invitrogen Corporation Recombinant methods for making reverse transcriptases and mutants thereof
ES2230701T3 (es) 1997-07-07 2005-05-01 Medical Research Council Procedimiento de seleccion in vitro.
US6136582A (en) * 1998-01-20 2000-10-24 The Trustees Of Columbia University In The City Reverse transcriptase of moloney murine leukemia virus with RNA polymerase activity
JP4193079B2 (ja) 1998-11-10 2008-12-10 東洋紡績株式会社 変異型逆転写酵素
EP1149170A1 (en) * 1999-01-15 2001-10-31 Molecular Biology Resources Biologically active reverse transcriptases
AU4741301A (en) 2000-03-15 2001-09-24 Invitrogen Corp High fidelity reverse transcriptases and uses thereof
CA2402452A1 (en) 2000-03-31 2001-10-11 Cambridge Antibody Technology Limited Improvements to ribosome display
US7078208B2 (en) 2000-05-26 2006-07-18 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
CN1430670A (zh) * 2000-05-26 2003-07-16 茵维特罗根公司 热稳定逆转录酶及其用途
GB0022458D0 (en) 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
US20070020622A1 (en) 2001-09-14 2007-01-25 Invitrogen Corporation DNA Polymerases and mutants thereof
GB0127564D0 (en) * 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
GB2388031B (en) 2002-04-30 2005-07-27 Abbott Lab A lancet removal tool
DK1534830T3 (da) * 2002-09-06 2011-02-14 Isogenica Ltd In vito-peptid-ekspressionsbibliotek
GB0221053D0 (en) 2002-09-11 2002-10-23 Medical Res Council Single-molecule in vitro evolution
WO2004024749A2 (en) * 2002-09-13 2004-03-25 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
EP1691792A4 (en) * 2003-11-24 2008-05-28 Yeda Res & Dev COMPOSITIONS AND PROCESSES FOR SORTING IN VITRO OF MOLECULE AND CELL LIBRARIES
WO2005071072A1 (en) 2004-01-13 2005-08-04 The Institute Of Biophysics Chinese Academy Of Sciences The recombinant murine leukemia virus reverse transcriptase, the gene encoding it and the method for expressing it
US7595179B2 (en) * 2004-04-19 2009-09-29 Applied Biosystems, Llc Recombinant reverse transcriptases
US20080004436A1 (en) 2004-11-15 2008-01-03 Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science Directed Evolution and Selection Using in Vitro Compartmentalization
JPWO2007011045A1 (ja) * 2005-07-20 2009-02-05 株式会社クラレ パージング剤
WO2007022045A2 (en) * 2005-08-10 2007-02-22 Stratagene California Mutant reverse transcriptase and methods of use
ES2404665T3 (es) 2006-09-06 2013-05-28 Medical Research Council Polimerasas de ADN que incorporan análogos de nucleótidos marcados con colorante

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101653775B1 (ko) * 2008-04-10 2016-09-05 써모 피셔 사이언티픽 발틱스 유에이비 핵산 제조 방법
KR101828902B1 (ko) * 2008-04-10 2018-02-13 써모 피셔 사이언티픽 발틱스 유에이비 핵산 제조 방법
KR101947101B1 (ko) * 2008-04-10 2019-02-12 써모 피셔 사이언티픽 발틱스 유에이비 핵산 제조 방법

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
J Virol. 2006 Sep;80(17):8379-89.
J Virol. 2007 Nov;81(22):12337-47.

Also Published As

Publication number Publication date
PL2281035T3 (pl) 2015-07-31
KR20160062213A (ko) 2016-06-01
ES2647272T3 (es) 2017-12-20
CA2721117A1 (en) 2009-10-15
US8835148B2 (en) 2014-09-16
GB0806562D0 (en) 2008-05-14
JP5642662B2 (ja) 2014-12-17
IL208578A (en) 2016-07-31
JP6473795B2 (ja) 2019-02-20
WO2009125006A3 (en) 2010-03-11
AU2009235368A1 (en) 2009-10-15
SG10201503327QA (en) 2015-06-29
EP3375870B1 (en) 2020-08-05
JP2018046841A (ja) 2018-03-29
US10287614B2 (en) 2019-05-14
JP6236563B2 (ja) 2017-11-22
EP2281035B1 (en) 2015-01-21
CN107058258A (zh) 2017-08-18
US20180298414A1 (en) 2018-10-18
KR20110065420A (ko) 2011-06-15
JP2014158473A (ja) 2014-09-04
JP6140792B2 (ja) 2017-05-31
IL246396B (en) 2018-04-30
US20130288925A1 (en) 2013-10-31
US20170298403A1 (en) 2017-10-19
EP3375870A1 (en) 2018-09-19
US8580548B2 (en) 2013-11-12
KR20180016635A (ko) 2018-02-14
IL208578A0 (en) 2010-12-30
AU2009235368B2 (en) 2014-01-16
JP2011516072A (ja) 2011-05-26
JP2016073298A (ja) 2016-05-12
US9683251B2 (en) 2017-06-20
EP2639300A2 (en) 2013-09-18
KR20190016132A (ko) 2019-02-15
JP2017169575A (ja) 2017-09-28
CN102057039A (zh) 2011-05-11
US20110065606A1 (en) 2011-03-17
KR101947101B1 (ko) 2019-02-12
KR101828902B1 (ko) 2018-02-13
US20120156752A1 (en) 2012-06-21
KR101653775B1 (ko) 2016-09-05
EP2639300A3 (en) 2014-03-26
US10358670B2 (en) 2019-07-23
EP3098308B1 (en) 2018-11-14
NZ588468A (en) 2012-10-26
WO2009125006A2 (en) 2009-10-15
EP2281035A2 (en) 2011-02-09
EP3098308A1 (en) 2016-11-30
EP2639300B1 (en) 2017-08-09

Similar Documents

Publication Publication Date Title
US10358670B2 (en) Production of nucleic acid
ES2914591T3 (es) Procedimientos novedosos para la producción de oligonucleótidos
CN112831484B (zh) T7-rna聚合酶突变体及其应用
JP2024028962A (ja) 不連続な複数の鋳型から相補的ＤＮＡ（ｃＤＮＡ）を順序だてて連続的に合成するための組成物およびその方法
ES2889548T3 (es) Método para introducir mutaciones
US10155944B2 (en) Tailed primer for cloned products used in library construction
Jo et al. A simple method for constructing a tagged protein

Legal Events

Date Code Title Description
A107 Divisional application of patent
PA0104 Divisional application for international application

St.27 status event code: A-0-1-A10-A18-div-PA0104

St.27 status event code: A-0-1-A10-A16-div-PA0104

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

A201 Request for examination
PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

St.27 status event code: A-1-2-D10-D22-exm-PE0701

GRNT Written decision to grant
PR0701 Registration of establishment

St.27 status event code: A-2-4-F10-F11-exm-PR0701

PR1002 Payment of registration fee

St.27 status event code: A-2-2-U10-U12-oth-PR1002

Fee payment year number: 1

PG1601 Publication of registration

St.27 status event code: A-4-4-Q10-Q13-nap-PG1601

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 4

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 5

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 6

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 7

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-OTH-PR1001 (AS PROVIDED BY THE NATIONAL OFFICE)

Year of fee payment: 7