JP5642662B2 - 核酸の生成 - Google Patents
核酸の生成 Download PDFInfo
- Publication number
- JP5642662B2 JP5642662B2 JP2011503454A JP2011503454A JP5642662B2 JP 5642662 B2 JP5642662 B2 JP 5642662B2 JP 2011503454 A JP2011503454 A JP 2011503454A JP 2011503454 A JP2011503454 A JP 2011503454A JP 5642662 B2 JP5642662 B2 JP 5642662B2
- Authority
- JP
- Japan
- Prior art keywords
- mrna
- enzyme
- reverse transcriptase
- nucleic acid
- pcr
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/162—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from virus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Virology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0806562.5A GB0806562D0 (en) | 2008-04-10 | 2008-04-10 | Production of nucleic acid |
| GB0806562.5 | 2008-04-10 | ||
| PCT/EP2009/054329 WO2009125006A2 (en) | 2008-04-10 | 2009-04-09 | Production of nucleic acid |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014059605A Division JP2014158473A (ja) | 2008-04-10 | 2014-03-24 | 核酸の生成 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2011516072A JP2011516072A (ja) | 2011-05-26 |
| JP5642662B2 true JP5642662B2 (ja) | 2014-12-17 |
Family
ID=39433452
Family Applications (5)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011503454A Active JP5642662B2 (ja) | 2008-04-10 | 2009-04-09 | 核酸の生成 |
| JP2014059605A Pending JP2014158473A (ja) | 2008-04-10 | 2014-03-24 | 核酸の生成 |
| JP2015230724A Active JP6140792B2 (ja) | 2008-04-10 | 2015-11-26 | 核酸の生成 |
| JP2017091035A Active JP6236563B2 (ja) | 2008-04-10 | 2017-05-01 | 核酸の生成 |
| JP2017208710A Active JP6473795B2 (ja) | 2008-04-10 | 2017-10-30 | 核酸の生成 |
Family Applications After (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014059605A Pending JP2014158473A (ja) | 2008-04-10 | 2014-03-24 | 核酸の生成 |
| JP2015230724A Active JP6140792B2 (ja) | 2008-04-10 | 2015-11-26 | 核酸の生成 |
| JP2017091035A Active JP6236563B2 (ja) | 2008-04-10 | 2017-05-01 | 核酸の生成 |
| JP2017208710A Active JP6473795B2 (ja) | 2008-04-10 | 2017-10-30 | 核酸の生成 |
Country Status (14)
| Country | Link |
|---|---|
| US (5) | US8835148B2 (https=) |
| EP (4) | EP2639300B1 (https=) |
| JP (5) | JP5642662B2 (https=) |
| KR (4) | KR102082768B1 (https=) |
| CN (2) | CN102057039A (https=) |
| AU (1) | AU2009235368B2 (https=) |
| CA (1) | CA2721117A1 (https=) |
| ES (1) | ES2647272T3 (https=) |
| GB (1) | GB0806562D0 (https=) |
| IL (2) | IL208578A (https=) |
| NZ (1) | NZ588468A (https=) |
| PL (1) | PL2281035T3 (https=) |
| SG (1) | SG10201503327QA (https=) |
| WO (1) | WO2009125006A2 (https=) |
Families Citing this family (100)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9771565B2 (en) * | 2000-05-26 | 2017-09-26 | Life Technologies Corporation | Thermostable reverse transcriptases and uses thereof |
| WO2004024749A2 (en) | 2002-09-13 | 2004-03-25 | Invitrogen Corporation | Thermostable reverse transcriptases and uses thereof |
| WO2008151023A2 (en) | 2007-06-01 | 2008-12-11 | Ibis Biosciences, Inc. | Methods and compositions for multiple displacement amplification of nucleic acids |
| GB0806562D0 (en) * | 2008-04-10 | 2008-05-14 | Fermentas Uab | Production of nucleic acid |
| EP2488656B1 (en) * | 2009-10-15 | 2015-06-03 | Ibis Biosciences, Inc. | Multiple displacement amplification |
| US8900814B2 (en) | 2010-08-13 | 2014-12-02 | Kyoto University | Variant reverse transcriptase |
| KR101818126B1 (ko) | 2011-02-09 | 2018-01-15 | (주)바이오니아 | 열안정성이 증가된 역전사효소 |
| US10323236B2 (en) | 2011-07-22 | 2019-06-18 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US9745571B2 (en) * | 2013-03-07 | 2017-08-29 | Bio-Rad Laboratories, Inc. | Repetitive reverse transcription partition assay |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| WO2015075842A1 (ja) * | 2013-11-25 | 2015-05-28 | 国立大学法人京都大学 | 変異型逆転写酵素 |
| US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
| JP2017503521A (ja) * | 2014-01-22 | 2017-02-02 | ライフ テクノロジーズ コーポレーション | 高温核酸合成で使用するための新規な逆転写酵素 |
| US10710081B2 (en) | 2014-03-14 | 2020-07-14 | Life Technologies Corporation | Integrated system for nucleic acid amplification and detection |
| CN104250642A (zh) * | 2014-04-30 | 2014-12-31 | 厦门安普利生物工程有限公司 | 一种提取纯化m-mlv逆转录酶的方法 |
| US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| RU2724998C2 (ru) | 2015-05-11 | 2020-06-29 | Иллюмина, Инк. | Платформа для обнаружения и анализа терапевтических агентов |
| IL310721B2 (en) | 2015-10-23 | 2025-11-01 | Harvard College | Nucleobase editors and uses thereof |
| US11046940B2 (en) | 2016-04-12 | 2021-06-29 | Solis Biodyne Ou | Synthetic reverse transcriptases and uses thereof |
| CN110214183A (zh) | 2016-08-03 | 2019-09-06 | 哈佛大学的校长及成员们 | 腺苷核碱基编辑器及其用途 |
| WO2018031683A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| US9580698B1 (en) * | 2016-09-23 | 2017-02-28 | New England Biolabs, Inc. | Mutant reverse transcriptase |
| KR102622411B1 (ko) | 2016-10-14 | 2024-01-10 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵염기 에디터의 aav 전달 |
| US11220677B2 (en) | 2016-12-14 | 2022-01-11 | Takara Bio Inc. | Heat-resistant reverse transcriptase mutant |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| US12390514B2 (en) | 2017-03-09 | 2025-08-19 | President And Fellows Of Harvard College | Cancer vaccine |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| US11542496B2 (en) | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| KR20240116572A (ko) | 2017-03-23 | 2024-07-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
| CN110709513B (zh) * | 2017-04-11 | 2023-10-03 | 豪夫迈·罗氏有限公司 | 具有热稳定性增加的突变型逆转录酶以及涉及其的产物、方法和用途 |
| WO2018200867A1 (en) * | 2017-04-26 | 2018-11-01 | 10X Genomics, Inc. | Mmlv reverse transcriptase variants |
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| EP3676376B1 (en) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
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| CN117106745B (zh) * | 2022-06-01 | 2025-02-14 | 北京擎科生物科技股份有限公司 | 逆转录突变体及其应用 |
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