CA2844868A1 - Rnai for the control of fungi and oomycetes by inhibiting saccharopine dehydrogenase gene - Google Patents

Rnai for the control of fungi and oomycetes by inhibiting saccharopine dehydrogenase gene Download PDF

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Publication number
CA2844868A1
CA2844868A1 CA2844868A CA2844868A CA2844868A1 CA 2844868 A1 CA2844868 A1 CA 2844868A1 CA 2844868 A CA2844868 A CA 2844868A CA 2844868 A CA2844868 A CA 2844868A CA 2844868 A1 CA2844868 A1 CA 2844868A1
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CA
Canada
Prior art keywords
plant
plants
sequence
gene
diseases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2844868A
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English (en)
French (fr)
Inventor
Thomas DELEBARRE
Cecile Dorme
Bernd Essigmann
Frederic Schmitt
Francois Villalba
Eric Paget
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Agricultural Solutions Seed US LLC
Original Assignee
Bayer Intellectual Property GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Intellectual Property GmbH filed Critical Bayer Intellectual Property GmbH
Publication of CA2844868A1 publication Critical patent/CA2844868A1/en
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/60—Isolated nucleic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/713—Double-stranded nucleic acids or oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y105/00—Oxidoreductases acting on the CH-NH group of donors (1.5)
    • C12Y105/01—Oxidoreductases acting on the CH-NH group of donors (1.5) with NAD+ or NADP+ as acceptor (1.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
CA2844868A 2011-10-04 2012-10-03 Rnai for the control of fungi and oomycetes by inhibiting saccharopine dehydrogenase gene Abandoned CA2844868A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP11356013.0 2011-10-04
EP11356013 2011-10-04
US201261661062P 2012-06-18 2012-06-18
US61/661,062 2012-06-18
PCT/EP2012/069521 WO2013050410A1 (en) 2011-10-04 2012-10-03 RNAi FOR THE CONTROL OF FUNGI AND OOMYCETES BY INHIBITING SACCHAROPINE DEHYDROGENASE GENE

Publications (1)

Publication Number Publication Date
CA2844868A1 true CA2844868A1 (en) 2013-04-11

Family

ID=48043183

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2844868A Abandoned CA2844868A1 (en) 2011-10-04 2012-10-03 Rnai for the control of fungi and oomycetes by inhibiting saccharopine dehydrogenase gene

Country Status (15)

Country Link
US (2) US20150082495A1 (enExample)
EP (1) EP2764101B1 (enExample)
JP (1) JP6255344B2 (enExample)
CN (1) CN103842507A (enExample)
AR (1) AR088113A1 (enExample)
AU (1) AU2012320554B2 (enExample)
BR (1) BR112014008059A2 (enExample)
CA (1) CA2844868A1 (enExample)
EA (1) EA028662B1 (enExample)
ES (1) ES2628436T3 (enExample)
IN (1) IN2014DN03473A (enExample)
PL (1) PL2764101T3 (enExample)
UA (1) UA115132C2 (enExample)
WO (1) WO2013050410A1 (enExample)
ZA (1) ZA201403196B (enExample)

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DE102012016009A1 (de) 2012-08-08 2014-02-13 Kws Saat Ag Transgene Pflanze der Art Solanum tuberosum mit Resistenz gegenüber Phytophthora
US20160058001A1 (en) 2013-04-19 2016-03-03 Bayer Cropscience Aktiengesellschaft Method for improved utilization of the production potential of transgenic plants
US11441147B2 (en) * 2013-07-10 2022-09-13 Basf Se RNAi for the control of phytopathogenic fungi and oomycetes by inhibiting the expression of CYP51 genes
EP3101135A1 (en) 2015-06-01 2016-12-07 Universität Hamburg Method of conferring resistance against a fusarium plant disease
WO2017191580A1 (en) * 2016-05-03 2017-11-09 University Of Manitoba Plants and methods for controlling fungal plant pathogens
CA3040728A1 (en) 2016-11-10 2018-05-17 Dow Agrosciences Llc Cytochrome b (cytb) nucleic acid molecules that control pathogens
WO2019074737A1 (en) 2017-10-10 2019-04-18 Dow Agrosciences Llc NUCLEIC ACID MOLECULES ALDEHYDE DEHYDROGENASE (ALDH1) THAT REGULATE PATHOGENS
CN112513033A (zh) 2018-06-04 2021-03-16 拜耳公司 除草活性的双环苯甲酰基吡唑
BR112021002698A2 (pt) * 2018-08-17 2021-08-10 Centre National De La Recherche Scientifique (Cnrs) métodos de biocontrole com base no rna para proteger plantas contra bactérias patogênica e/ou promover efeitos benéficos de bactérias simbióticas e comensais
CN111647572A (zh) * 2020-06-01 2020-09-11 中国科学院天津工业生物技术研究所 源于里氏木霉的还原酶及其编码基因和用途
WO2022146195A1 (ru) * 2020-12-30 2022-07-07 Общество с ограниченной ответственностью "Международная лаборатория "Резистом" ИНТЕРФЕРИРУЮЩАЯ дцРНК ДЛЯ ЗАЩИТЫ РАСТЕНИЙ ОТ ФИТОФТОРОЗА
EP4304353A4 (en) * 2021-03-08 2025-02-12 Greenlight Biosciences, Inc. RNA-BASED CONTROL OF POWDERY MILDEW
KR102727673B1 (ko) * 2021-09-29 2024-11-07 서울대학교산학협력단 감자역병균의 증식을 억제하는 Avr8 표적 dsRNA 및 이의 용도
KR102863861B1 (ko) * 2023-03-29 2025-09-24 서울대학교산학협력단 감자역병균의 증식을 억제하는 Avr3a 표적 dsRNA 및 이의 용도
CN119391501B (zh) * 2024-08-30 2025-10-10 华南农业大学 一种用于宿主介导的丛枝菌根真菌基因过表达的装置及方法

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