CA2268384A1 - Chrysosporium cellulase and methods of use - Google Patents
Chrysosporium cellulase and methods of use Download PDFInfo
- Publication number
- CA2268384A1 CA2268384A1 CA002268384A CA2268384A CA2268384A1 CA 2268384 A1 CA2268384 A1 CA 2268384A1 CA 002268384 A CA002268384 A CA 002268384A CA 2268384 A CA2268384 A CA 2268384A CA 2268384 A1 CA2268384 A1 CA 2268384A1
- Authority
- CA
- Canada
- Prior art keywords
- chrysosporium
- cellulase
- fungus
- composition
- genus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
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- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06L—DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS, YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS; BLEACHING LEATHER OR FURS
- D06L4/00—Bleaching fibres, filaments, threads, yarns, fabrics, feathers or made-up fibrous goods; Bleaching leather or furs
- D06L4/40—Bleaching fibres, filaments, threads, yarns, fabrics, feathers or made-up fibrous goods; Bleaching leather or furs using enzymes
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- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
- D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms
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- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06P—DYEING OR PRINTING TEXTILES; DYEING LEATHER, FURS OR SOLID MACROMOLECULAR SUBSTANCES IN ANY FORM
- D06P5/00—Other features in dyeing or printing textiles, or dyeing leather, furs, or solid macromolecular substances in any form
- D06P5/02—After-treatment
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
- C11D2111/10—Objects to be cleaned
- C11D2111/12—Soft surfaces, e.g. textile
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- C12R2001/00—Microorganisms ; Processes using microorganisms
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- D06M2101/00—Chemical constitution of the fibres, threads, yarns, fabrics or fibrous goods made from such materials, to be treated
- D06M2101/02—Natural fibres, other than mineral fibres
- D06M2101/04—Vegetal fibres
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Applications Claiming Priority (3)
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| US08/731,170 US5811381A (en) | 1996-10-10 | 1996-10-10 | Cellulase compositions and methods of use |
| US08/731,170 | 1996-10-10 | ||
| PCT/US1997/017669 WO1998015633A1 (en) | 1996-10-10 | 1997-09-30 | Chrysosporium cellulase and methods of use |
Publications (1)
| Publication Number | Publication Date |
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| CA2268384A1 true CA2268384A1 (en) | 1998-04-16 |
Family
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Family Applications (1)
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| CA002268384A Abandoned CA2268384A1 (en) | 1996-10-10 | 1997-09-30 | Chrysosporium cellulase and methods of use |
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| JP (3) | JP2001506845A (enExample) |
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Families Citing this family (191)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19515072A1 (de) * | 1995-04-28 | 1996-10-31 | Cognis Bio Umwelt | Cellulasehaltiges Waschmittel |
| WO1997018286A1 (en) * | 1995-11-15 | 1997-05-22 | Novo Nordisk A/S | A process for combined desizing and 'stone-washing' of dyed denim |
| US7883872B2 (en) * | 1996-10-10 | 2011-02-08 | Dyadic International (Usa), Inc. | Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose |
| US5811381A (en) * | 1996-10-10 | 1998-09-22 | Mark A. Emalfarb | Cellulase compositions and methods of use |
| DE19732750A1 (de) | 1997-07-30 | 1999-02-04 | Henkel Kgaa | Glucanasehaltiges Reinigungsmittel für harte Oberflächen |
| DE19732749A1 (de) * | 1997-07-30 | 1999-02-04 | Henkel Kgaa | Glucanasehaltiges Waschmittel |
| US6248134B1 (en) * | 1998-01-12 | 2001-06-19 | Novozymes A/S | Process for removal of excess dye from printed or dyed fabric or yarn |
| US6146428A (en) * | 1998-04-03 | 2000-11-14 | Novo Nordisk A/S | Enzymatic treatment of denim |
| AU771539C (en) * | 1998-10-06 | 2005-01-13 | Dyadic International (Usa), Inc. | Transformation system in the field of filamentous fungal hosts |
| US7070847B2 (en) * | 1999-02-18 | 2006-07-04 | Milliken & Company | Abraded fabrics exhibiting excellent hand properties and simultaneously high fill strength retention |
| US6233795B1 (en) * | 1999-02-18 | 2001-05-22 | Milliken & Company | Face finishing of cotton-containing fabrics containing immobilized fibers |
| AU4352700A (en) * | 1999-10-06 | 2001-05-10 | Mark Aaron Emalfarb | High-throughput screening of expressed dna libraries in filamentous fungi |
| HUP0300469A2 (hu) * | 2000-04-13 | 2003-06-28 | Emalfarb, Mark Aaron | Új, expressziós szabályzó szekvenciák és expressziós termékek fonalas gombákban |
| KR20020026456A (ko) * | 2000-04-13 | 2002-04-10 | 에말파브 마크 아론 | 사상균에서 발현된 ｄｎａ 라이브러리의 고산출량 스크리닝 |
| US6716775B1 (en) * | 2000-05-12 | 2004-04-06 | Milliken & Company | Range-dyed face finished fabrics exhibiting non-directional surface fiber characteristics |
| EP1297004A2 (en) | 2000-06-15 | 2003-04-02 | Prokaria ehf. | Thermostable cellulase |
| US7319112B2 (en) | 2000-07-14 | 2008-01-15 | The Procter & Gamble Co. | Non-halogenated antibacterial agents and processes for making same |
| CN1172053C (zh) * | 2001-02-09 | 2004-10-20 | 广东溢达纺织有限公司 | 免烫耐洗纯棉针织物的生产工艺 |
| DE10202390A1 (de) * | 2002-01-23 | 2003-09-25 | Henkel Kgaa | Kombination von Cellulasen und spezieller Cellulose in Waschmitteln |
| US7799141B2 (en) * | 2003-06-27 | 2010-09-21 | Lam Research Corporation | Method and system for using a two-phases substrate cleaning compound |
| US7169257B2 (en) * | 2003-11-12 | 2007-01-30 | Kemira Chemicals, Inc. | Method of deinking waste paper using a reduced alkali system |
| US8008056B2 (en) * | 2004-12-30 | 2011-08-30 | Danisco Us Inc. | Variant Hypocrea jecorina CBH2 cellulases |
| FI120045B (fi) * | 2005-12-22 | 2009-06-15 | Roal Oy | Selluloosamateriaalin käsittely ja siinä käyttökelpoiset entsyymit |
| US8143482B2 (en) | 2006-03-20 | 2012-03-27 | Novozymes, Inc. | Polypeptides having endoglucanase activity and polynucleotides encoding same |
| EP2004817A2 (en) * | 2006-03-30 | 2008-12-24 | Novozymes, Inc. | Polypeptides having endoglucanase activity and polynucleotides encoding same |
| WO2008023386A2 (en) * | 2006-08-25 | 2008-02-28 | Advanced Enzyme Technologies Limited | Novel compositions for biobleaching coupled with stone washing of indigo dyed denims and process thereof |
| US8680252B2 (en) | 2006-12-10 | 2014-03-25 | Dyadic International (Usa), Inc. | Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi |
| US9862956B2 (en) | 2006-12-10 | 2018-01-09 | Danisco Us Inc. | Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi |
| DE102007001402A1 (de) * | 2007-01-01 | 2008-07-03 | Corpus.E Ag | Optische Erfassung der Raumform von räumlich positionierten Füßen |
| SG148934A1 (en) * | 2007-06-11 | 2009-01-29 | Novozymes As | A process for combined biopolishing and bleach clean-up |
| US7923236B2 (en) * | 2007-08-02 | 2011-04-12 | Dyadic International (Usa), Inc. | Fungal enzymes |
| WO2009033071A2 (en) | 2007-09-07 | 2009-03-12 | Dyadic International, Inc. | Novel fungal enzymes |
| BRPI0821230A2 (pt) | 2007-12-19 | 2019-09-24 | Novozymes As | "constructo de ácido nucleico, cédula microbiana hospedeira recombinante, métodos para priduzir um polipeptídeo tendo atividade intensificadora celulolítica, para produzir um mutante de uma cédula precursora, para inibir a expressão de um polipeptídeo com atividade de intensificação celulolítica em uma célula, para produzir uma proteína, para degradar ou converter um material celulósico, e, para fermentar um material celulósico" |
| WO2010039539A2 (en) * | 2008-09-23 | 2010-04-08 | Modular Genetics, Inc. | Growth of microorganisms in cellulosic media |
| BRPI0921966B1 (pt) | 2008-11-21 | 2019-05-07 | Lallemand Hungary Liquidity Management Llc. | Célula hospedeira de levedura termotolerante, método de fermentar celulose e cultura |
| WO2010065830A1 (en) | 2008-12-04 | 2010-06-10 | Novozymes, Inc. | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
| WO2010065908A2 (en) * | 2008-12-05 | 2010-06-10 | Catchmark Jeffrey M | Degradable biomolecule compositions |
| EP2379732A2 (en) | 2008-12-19 | 2011-10-26 | Novozymes Inc. | Methods for increasing enzymatic hydrolysis of cellulosic material in the presence of a peroxidase |
| BRPI0923026A2 (pt) | 2008-12-19 | 2017-03-28 | Novozymes Inc | métodos para degradar ou converter um material celulósico, para produzir um produto de fermentação, e para fermentar um material celulósico. |
| US8337663B2 (en) | 2008-12-19 | 2012-12-25 | Novozymes, Inc. | Methods for increasing hydrolysis of cellulosic material |
| WO2010080527A1 (en) | 2008-12-19 | 2010-07-15 | Novozymes, Inc. | Methods for determining cellulolytic enhancing activity of a polypeptide |
| US8604277B2 (en) | 2009-01-28 | 2013-12-10 | Novozymes, Inc. | Polypeptides having beta-glucosidase activity and polynucleotides encoding same |
| US8629324B2 (en) | 2009-01-30 | 2014-01-14 | Novozymes, Inc. | Polypeptides having expansin activity and polynucleotides encoding same |
| CA2755417C (en) | 2009-03-16 | 2018-11-13 | Dyadic Nederland B.V. | New fungal production system |
| DK2411511T3 (en) | 2009-03-24 | 2018-11-26 | Novozymes As | POLYPEPTIDES WITH ACETYLXYLANESTERASE ACTIVITY AND POLYNUCLEOTIDES CODING THEM |
| BRPI1010239A2 (pt) | 2009-03-31 | 2016-10-11 | Codexis Inc | endoglucanases aprimoradas, derivados e seus usos |
| WO2010138754A1 (en) | 2009-05-29 | 2010-12-02 | Novozymes, Inc. | Methods for enhancing the degradation or conversion of cellulosic material |
| CN104480088A (zh) | 2009-06-02 | 2015-04-01 | 诺维信股份有限公司 | 具有纤维二糖水解酶活性的多肽和编码该多肽的多核苷酸 |
| BRPI1010696A2 (pt) | 2009-06-16 | 2016-10-11 | Codexis Inc | método de produção de um polipeptídeo de b-glicosidade com termoatividade aperfeiçoada, polipeptídeo, proteína e seus usos |
| EP2451957B1 (en) | 2009-07-07 | 2017-11-15 | Novozymes Inc. | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
| KR101104068B1 (ko) * | 2009-09-15 | 2012-01-12 | 한국생명공학연구원 | 마늘 유래의 엔도-1,4-베타-글루카나아제 유전자 및 이의 용도 |
| BR112012006032A2 (pt) | 2009-09-17 | 2015-09-08 | Novozymes Inc | polipetídeo isolado, composição, célula hospedeira recombinante, métodos para produzir o polipetídeo, um polipetídeo tendo atividade realçadora celulolítica, um mutante de uma célula precursora, uma proteína e um produto de fermentação, planta transgênica, parte de planta ou célula de planta, molécula de rna inibidora de filamento duplo(rsrna), e, métodos para inibir a expressão de um polipetídeo tendo atividade realçadora celulolítica em uma célula, degradar ou converter um polipetídeo isolado, composição, célula hospedeira recombinante, métodos para produzir o polipetídeo, um polipetídeo tendo atividade realçadora celulolítica, um mutante de uma célula precursora, uma proteína e um produto de fermentação, planta transgênica, parte de planta ou célula de planta, molécula de rna inibidora de filamento duplo (dsrna), e, métodos para inibir a expressão de um polipetídeo tendo atividade realçadora celulolítica em uma célula, degradar ou converter um material celulósico e fermentar um material celulósico. |
| EP2478095A1 (en) | 2009-09-18 | 2012-07-25 | Novozymes Inc. | Polypeptides having beta-glucosidase activity and polynucleotides encoding same |
| EP2480660B1 (en) | 2009-09-23 | 2020-08-05 | Danisco US Inc. | Novel glycosyl hydrolase enzymes and uses thereof |
| WO2011041397A1 (en) | 2009-09-29 | 2011-04-07 | Novozymes, Inc. | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
| US8211665B2 (en) | 2009-09-29 | 2012-07-03 | Novozymes, Inc. | Polypeptides having xylanase activity and polynucleotides encoding same |
| CA2776170C (en) | 2009-09-30 | 2016-01-05 | Codexis, Inc. | Recombinant c1 .beta.-glucosidase for production of sugars from cellulosic biomass |
| EP2977382A3 (en) | 2009-09-30 | 2016-05-11 | Novozymes Inc. | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
| US8586829B2 (en) | 2009-09-30 | 2013-11-19 | Novozymes A/S | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
| US20110086236A1 (en) * | 2009-10-13 | 2011-04-14 | The Penn State Research Foundation | Composites containing polypeptides attached to polysaccharides and molecules |
| EP2491122A1 (en) | 2009-10-23 | 2012-08-29 | Novozymes Inc. | Cellobiohydrolase variants and polynucleotides encoding same |
| BR112012006847A2 (pt) | 2009-10-29 | 2015-09-08 | Novozymes As | polipeptídeo polinucleotídeo, célula hospedeira recombinante, métodos para produzir o polipeptídeo para produzir um mutante de uma célula parental, para inibir a expressão de um polipeptídeo, para produzir uma proteína, para degradar ou converter um material celulósico, para produzir um produto de fermentação, para produzir um produto de fermentação e pra fermentar um material celulósico, planta transgênica, parte da planta ou célula da planta, molécula de rna inibitória de filamento duplo, e, composição. |
| ES2574054T3 (es) | 2009-11-06 | 2016-06-14 | Novozymes, Inc. | Polipéptidos con actividad de xilanasa y polinucleótidos que los codifican |
| BR112012008260A2 (pt) | 2009-11-06 | 2015-09-15 | Novozymes Inc E Novozymes As | polipeptídeo, polinucleotídeo, métodos para produzir o polipeptídeo, para produzir um mutante de uma célula precursora, para inibir a expressão de um polipeptídeo, para produzir uma proteína, para degradar ou converter um material celulósico, para produzir um produto de fermentação, para fermentar um material celulósico, planta transgênica, parte da planta ou célula da planta,e, molécula de rna inibitória de filamento duplo. |
| EP2706119B1 (en) | 2009-11-25 | 2015-08-12 | Codexis, Inc. | Recombinant beta-glucosidase variants for production of soluble sugars from cellulosic biomass |
| BR112012012192A2 (pt) | 2009-11-25 | 2017-07-04 | Codexis Inc | b-glicosidades variantes recombinantes thermoascus aurantiacus para produção de açúcares fermentáveis a partir da biomassa celulósica |
| CN107287250A (zh) | 2009-12-23 | 2017-10-24 | 丹尼斯科美国公司 | 提高同步糖化发酵反应效率的方法 |
| US8828701B2 (en) | 2010-03-31 | 2014-09-09 | Novozymes, Inc. | Cellobiohydrolase variants and polynucleotides encoding same |
| US8581042B2 (en) | 2010-06-30 | 2013-11-12 | Novozymes A/S | Polypeptides having beta-glucosidase activity and polynucleotides encoding same |
| MX2013001540A (es) | 2010-08-12 | 2013-04-24 | Novozymes Inc | Composiciones que comprende un polipeptido que tiene actividad de incremento celulolititico y un compuesto de quinona, y uso de las mismas. |
| WO2012024662A2 (en) | 2010-08-20 | 2012-02-23 | Codexis, Inc. | Expression constructs comprising fungal promoters |
| EP2606131B1 (en) | 2010-08-20 | 2017-03-08 | Codexis, Inc. | Use of glycoside hydrolase 61 family proteins in processing of cellulose |
| WO2012030844A1 (en) | 2010-08-30 | 2012-03-08 | Novozymes A/S | Polypeptides having endoglucanase activity and polynucleotides encoding same |
| WO2012030845A2 (en) | 2010-08-30 | 2012-03-08 | Novozymes A/S | Polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and polynucleotides encoding same |
| US8624082B2 (en) | 2010-08-30 | 2014-01-07 | Novozymes A/S | Polypeptides having xylanase activity and polynucleotides encoding same |
| US9187742B2 (en) | 2010-08-30 | 2015-11-17 | Novozymes, Inc. | Polypeptides having cellobiohydrolase activity and polynucleotides encoding same |
| WO2012030799A1 (en) | 2010-08-30 | 2012-03-08 | Novozymes A/S | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
| WO2012030858A2 (en) | 2010-08-30 | 2012-03-08 | Novozymes A/S | Polypeptides having hemicellulolytic activity and polynucleotides encoding same |
| ES2594528T3 (es) | 2010-09-30 | 2016-12-20 | Novozymes, Inc. | Variantes de polipéptidos con actividad de mejora celulolítica y polinucleótidos que las codifican |
| BR112013007699B1 (pt) | 2010-09-30 | 2021-12-21 | Novozymes, Inc. | Variante, polinucleotídeo isolado, célula hospedeira microbiana recombinante, métodos para produzir uma variante, para degradar um material celulósico, para produzir um produto de fermentação, para fermentar um material celulósico, e, composição enzimática, formulação do caldo completo ou composição de cultura de células |
| DK2622069T3 (en) | 2010-10-01 | 2016-02-22 | Novozymes Inc | Beta-glucosidasevarianter and polynucleotides encoding them |
| WO2012058293A1 (en) | 2010-10-26 | 2012-05-03 | Novozymes North America, Inc. | Methods of saccharifying sugarcane trash |
| EP2635690B1 (en) | 2010-11-02 | 2016-02-03 | Codexis, Inc. | Compositions and methods for production of fermentable sugars |
| PL2635671T3 (pl) | 2010-11-02 | 2017-02-28 | Codexis, Inc. | Ulepszone szczepy grzybowe |
| EP2635689B1 (en) | 2010-11-02 | 2015-04-15 | Novozymes, Inc. | Methods of pretreating cellulosic material with a gh61 polypeptide |
| US9212354B2 (en) | 2010-11-04 | 2015-12-15 | Novozymes Inc. | Polypeptides having cellobiohydrolase activitiy and polynucleotides encoding same |
| WO2012062220A1 (en) | 2010-11-12 | 2012-05-18 | Novozymes A/S | Polypeptides having endoglucanase activity and polynucleotides encoding same |
| WO2012068509A1 (en) | 2010-11-18 | 2012-05-24 | Novozymes, Inc. | Chimeric polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
| EP2649188A1 (en) | 2010-12-06 | 2013-10-16 | Novozymes North America, Inc. | Methods of hydrolyzing oligomers in hemicellulosic liquor |
| CN102154870B (zh) * | 2010-12-28 | 2012-09-05 | 宜宾长毅浆粕有限责任公司 | 一种制备高聚合度浆粕的方法 |
| WO2012103322A1 (en) | 2011-01-26 | 2012-08-02 | Novozymes A/S | Polypeptides having endoglucanase activity and polynucleotides encoding same |
| BR112013018695B1 (pt) | 2011-01-26 | 2021-03-30 | Novozymes A / S | Célula hospedeira microbiana recombinante, processos para produzir um polipeptídeo, para produzir uma proteína, para degradar um material celulósico, e, construto de ácido nucleico ou vetor de expressão |
| WO2012103350A1 (en) | 2011-01-26 | 2012-08-02 | Novozymes A/S | Polypeptides having cellobiohydrolase activity and polynucleotides encoding same |
| EP3235903B1 (en) | 2011-01-26 | 2021-07-07 | Novozymes A/S | Polypeptides having cellobiohydrolase activity and polynucleotides encoding same |
| DK2668266T3 (en) | 2011-01-26 | 2018-03-26 | Novozymes Inc | POLYPEPTIDES WITH CELLOBIO HYDROASE ACTIVITY AND POLYNUCLEOTIDES CODING THEM |
| BR112013019324B1 (pt) | 2011-01-31 | 2021-06-08 | Novozymes North America, Inc | processos para refinamento enzimático de um material celulósico pré-tratado |
| CA2824494A1 (en) * | 2011-01-31 | 2012-08-09 | Dsm Ip Assets B.V. | Mutant cellobiohydrolase |
| BR112013020264A2 (pt) | 2011-02-09 | 2016-09-20 | Novozymes As | composição de eliminação de boboto, uso da composição de eliminação de borboto, e, micróbio geneticamente modificado |
| EP2678352B1 (en) | 2011-02-23 | 2017-12-06 | Novozymes, Inc. | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
| DK3339442T3 (da) | 2011-03-09 | 2022-06-20 | Novozymes Inc | Fremgangsmåder til forøgelse af den cellulolyseforbedrende aktivitet af et polypeptid |
| US9409958B2 (en) | 2011-03-10 | 2016-08-09 | Novozymes, Inc. | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
| EP2686427A1 (en) | 2011-03-17 | 2014-01-22 | Danisco US Inc. | Cellulase compositions and methods of using the same for improved conversion of lignocellulosic biomass into fermentable sugars |
| EP2686434B1 (en) | 2011-03-17 | 2021-07-14 | Danisco US Inc. | Method for reducing viscosity in saccharification process |
| WO2012130120A1 (en) | 2011-03-25 | 2012-10-04 | Novozymes A/S | Method for degrading or converting cellulosic material |
| US20140080182A1 (en) | 2011-03-31 | 2014-03-20 | Novozymes, Inc. | Cellulose Binding Domain Variants and Polynucleotides Encoding Same |
| US9410136B2 (en) | 2011-03-31 | 2016-08-09 | Novozymes, Inc. | Methods for enhancing the degradation or conversion of cellulosic material |
| DK2702153T3 (en) | 2011-04-28 | 2019-03-18 | Novozymes Inc | Polypeptides with endoglucanase activity and polynucleotides encoding them |
| CN103797126A (zh) | 2011-04-29 | 2014-05-14 | 诺维信股份有限公司 | 用于增强纤维素材料的降解或转化的方法 |
| EP2710132A1 (en) | 2011-05-19 | 2014-03-26 | Novozymes, Inc. | Methods for enhancing the degradation of cellulosic material with chitin binding proteins |
| US8993286B2 (en) | 2011-05-19 | 2015-03-31 | Novozymes, Inc. | Methods for enhancing the degradation of cellulosic material with chitin binding proteins |
| US20140147895A1 (en) | 2011-07-22 | 2014-05-29 | Novozymes A/S | Processes for Pretreating Cellulosic Material and Improving Hydrolysis Thereof |
| EP2739728B1 (en) | 2011-08-04 | 2017-07-12 | Novozymes A/S | Polypeptides having endoglucanase activity and polynucleotides encoding same |
| WO2013019827A2 (en) | 2011-08-04 | 2013-02-07 | Novozymes A/S | Polypeptides having xylanase activity and polynucleotides encoding same |
| BR112014004171A2 (pt) | 2011-08-22 | 2018-11-06 | Codexis Inc | variantes da proteína glicósideo hidrolase gh61 e cofatores que aumentam a atividade de gh61 |
| IN2014CN02136A (enExample) | 2011-08-24 | 2015-05-29 | Novozymes Inc | |
| US9404101B2 (en) | 2011-08-24 | 2016-08-02 | Novozymes, Inc. | Methods for obtaining positive transformants of a filamentous fungal host cell |
| US20140363846A1 (en) * | 2011-08-26 | 2014-12-11 | Iogen Energy Corporation | Process for producing cellulase mixtures from myceliophthora and related organisms |
| US9670510B2 (en) | 2011-09-13 | 2017-06-06 | Novozymes A/S | Methods of hydrolyzing and fermenting cellulosic material |
| WO2013043317A1 (en) * | 2011-09-20 | 2013-03-28 | Codexis, Inc. | Endoglucanase 1b |
| WO2013043910A1 (en) | 2011-09-20 | 2013-03-28 | Novozymes A/S | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
| BR112014007719A2 (pt) | 2011-09-30 | 2017-06-13 | Codexis Inc | proteases fúngicas |
| DK2760885T3 (en) | 2011-09-30 | 2017-10-30 | Novozymes Inc | CHEMICAL POLYPEPTIDES WITH BETA-GLUCOSIDASE ACTIVITY AND POLYNUCLEOTIDES CODING THEM |
| DK2773656T3 (da) | 2011-10-31 | 2019-09-09 | Novozymes Inc | Polypeptider med cellulolyseforbedrende aktivitet og polynukleotider, som koder for dem |
| US9562222B2 (en) | 2011-11-18 | 2017-02-07 | Novozymes A/S | Polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and polynucleotides encoding same |
| EP3219794B1 (en) | 2011-11-21 | 2019-09-11 | Novozymes A/S | Gh61 polypeptide variants and polynucleotides encoding same |
| US11253818B2 (en) | 2011-11-21 | 2022-02-22 | Toray Industries, Inc. | Method for producing cellulase and apparatus for said method |
| WO2013075644A1 (en) | 2011-11-22 | 2013-05-30 | Novozymes, Inc. | Polypeptides having beta-xylosidase activity and polynucleotides encoding same |
| US9624481B2 (en) | 2011-12-01 | 2017-04-18 | Novozymes, Inc. | Polypeptides having beta-xylosidase activity and polynucleotides encoding same |
| EP3272862A1 (en) | 2011-12-16 | 2018-01-24 | Novozymes, Inc. | Polypeptides having laccase activity and polynucleotides encoding same |
| WO2013096369A1 (en) | 2011-12-19 | 2013-06-27 | Novozymes A/S | Processes and compositions for increasing the digestibility of cellulosic materials |
| EP2794870A4 (en) | 2011-12-19 | 2015-06-17 | Novozymes Inc | POLYPEPTIDES WITH XYLANASE ACTIVITY AND POLYNUCLEOTIDES THAT CODE |
| DK2794869T3 (en) | 2011-12-20 | 2018-01-02 | Novozymes Inc | CELLOBIO HYDROLASE VARIABLES AND POLYNUCLEOTIDES CODING THEM |
| US9611462B2 (en) | 2011-12-20 | 2017-04-04 | Codexis, Inc. | Endoglucanase 1B (EG1B) variants |
| EP2794899A1 (en) | 2011-12-21 | 2014-10-29 | Novozymes, Inc. | Methods for determining the degradation of a biomass material |
| BR112014026268A2 (pt) | 2012-04-23 | 2017-07-18 | Novozymes As | polipeptídeo isolado, composição, polinucleotídeo isolado, construção de ácido nucleico ou vetor de expressão, célula hospedeira recombinante, e, métodos de produção de um polipeptídeo e de degradação ou conversão de um material celulósico |
| BR112014024804A2 (pt) | 2012-04-23 | 2017-07-11 | Novozymes As | polipeptídeo isolado tendo atividade de glucuronil esterase, composição, polinucleotídeo isolado, construção de ácido nucleico ou vetor de expressão, célula hospedeira recombinante, métodos para a produção de um polipeptídeo, para a degradação ou conversão de um material celulósico e para a produção de um produto de fermentação |
| CN113234695A (zh) | 2012-04-27 | 2021-08-10 | 诺维信股份有限公司 | Gh61多肽变体以及编码其的多核苷酸 |
| WO2013188305A2 (en) | 2012-06-11 | 2013-12-19 | Codexis, Inc. | Fungal beta-xylosidase variants |
| WO2014092832A2 (en) | 2012-09-19 | 2014-06-19 | Novozymes, Inc. | Methods for enhancing the degradation or conversion of cellulosic material |
| WO2014058896A1 (en) | 2012-10-08 | 2014-04-17 | Novozymes A/S | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
| US20150275194A1 (en) | 2012-10-24 | 2015-10-01 | Novozymes A/S | Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same |
| CN102936601A (zh) * | 2012-11-22 | 2013-02-20 | 徐州工程学院 | 一种内切葡聚糖酶编码基因和重组酶及应用 |
| WO2014093835A1 (en) | 2012-12-14 | 2014-06-19 | Novozymes A/S | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
| US20150337280A1 (en) | 2012-12-19 | 2015-11-26 | Novozymes A/S | Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same |
| BR112015016329A2 (pt) * | 2013-01-11 | 2017-07-11 | Codexis Inc | métodos para produzir pelo menos uma enzima, uma mistura de enzima, açúcares fermentáveis e pelo menos um produto final a partir de pelo menos um substrato celulósico |
| CN105164254B (zh) | 2013-03-08 | 2019-06-28 | 诺维信公司 | 纤维二糖水解酶变体和编码它们的多核苷酸 |
| EP3024887B1 (en) | 2013-07-26 | 2023-11-22 | The Penn State Research Foundation | Method for preparation of polymer compositions and coatings |
| WO2015150372A1 (en) | 2014-04-01 | 2015-10-08 | Dupont Nutrition Biosciences Aps | Method for increasing crude palm oil yields |
| WO2016037096A1 (en) | 2014-09-05 | 2016-03-10 | Novozymes A/S | Carbohydrate binding module variants and polynucleotides encoding same |
| BR112017013025A2 (pt) | 2014-12-19 | 2018-01-02 | Dupont Nutrition Biosci Aps | recuperação de óleo da lama de palma |
| PL3640336T3 (pl) | 2015-01-28 | 2022-08-01 | Dsm Ip Assets B.V. | Sposób enzymatycznej hydrolizy materiału lignocelulozowego i fermentacji cukrów |
| DK3250698T3 (da) | 2015-01-28 | 2020-02-24 | Dsm Ip Assets Bv | Fremgangsmåde til enzymatisk hydrolyse af lignocellulosemateriale og fermentering af sukker |
| WO2016120298A1 (en) | 2015-01-28 | 2016-08-04 | Dsm Ip Assets B.V. | Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars |
| DK3262165T3 (da) | 2015-02-24 | 2020-08-24 | Novozymes As | Cellobiohydrolasevarianter og polynukleotider, som koder for dem |
| US10266863B2 (en) | 2015-03-11 | 2019-04-23 | Genencor International B.V. | Enzymatic activity of lytic polysaccharide monooxygenase |
| EP3067428A1 (en) | 2015-03-12 | 2016-09-14 | BETA RENEWABLES S.p.A. | A process for producing a hydrolyzed mixture from a pre-treated ligno-cellulosic slurry comprising a slurry liquid and slurry solids |
| WO2016169892A1 (en) | 2015-04-20 | 2016-10-27 | Dsm Ip Assets B.V. | Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars |
| WO2016169893A1 (en) | 2015-04-20 | 2016-10-27 | Dsm Ip Assets B.V. | Whole fermentation broth |
| WO2016207144A1 (en) | 2015-06-22 | 2016-12-29 | Dsm Ip Assets B.V. | Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars |
| EP3313999B1 (en) | 2015-06-24 | 2021-04-28 | Genencor International B.V. | Polypeptides having demethylating activity |
| DK3419992T3 (da) | 2016-02-22 | 2021-02-15 | Danisco Us Inc | Svampesystem til proteinproduktion med højt udbytte |
| CA3026325A1 (en) | 2016-06-09 | 2017-12-14 | Dsm Ip Assets B.V. | Seed train for large scale enzyme production |
| US20190330612A1 (en) | 2016-06-30 | 2019-10-31 | Danisco Us Inc | Aspartic proteases |
| US10913938B2 (en) | 2016-07-29 | 2021-02-09 | Dsm Ip Assets B.V. | Polypeptides having cellulolytic enhancing activity and uses thereof |
| BR112019004915A2 (pt) | 2016-09-16 | 2019-06-25 | Basf Se | método de modificação de pasta de fabricação de papel, pasta de fibra modificada com enzima, pasta de fibra de madeira macia modificada com enzima, e, produto de pasta ou folha contínua de papel. |
| MY198644A (en) | 2016-11-24 | 2023-09-13 | Dsm Ip Assets Bv | Enzyme composition |
| WO2018096017A1 (en) | 2016-11-24 | 2018-05-31 | Dsm Ip Assets B.V. | Enzyme composition |
| WO2018185071A1 (en) | 2017-04-03 | 2018-10-11 | Dsm Ip Assets B.V. | Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars |
| US11319559B2 (en) | 2017-10-09 | 2022-05-03 | Dsm Ip Assets B.V. | Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars |
| WO2019086370A1 (en) | 2017-10-30 | 2019-05-09 | Dsm Ip Assets B.V. | Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars |
| CA3078156A1 (en) | 2017-10-30 | 2019-05-09 | Dsm Ip Assets B.V. | Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars |
| WO2019185681A1 (en) | 2018-03-28 | 2019-10-03 | Dsm Ip Assets B.V. | Enzyme composition |
| US11193145B2 (en) | 2018-03-28 | 2021-12-07 | Dsm Ip Assets B.V. | Enzyme composition |
| BR112020023198A2 (pt) | 2018-05-17 | 2021-02-09 | Dsm Ip Assets B.V. | processo para produção de um polipeptídeo |
| CN112204151B (zh) | 2018-05-30 | 2024-06-11 | 维尔萨利斯股份公司 | 从碳水化合物材料产生糖的方法 |
| CN108797130B (zh) * | 2018-07-04 | 2021-07-16 | 湖南尤特尔生化有限公司 | 一种毛巾退浆抛光一浴加工工艺方法 |
| WO2020058248A1 (en) | 2018-09-18 | 2020-03-26 | Dsm Ip Assets B.V. | Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars |
| WO2020058253A1 (en) | 2018-09-18 | 2020-03-26 | Dsm Ip Assets B.V. | Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars |
| WO2020058249A1 (en) | 2018-09-18 | 2020-03-26 | Dsm Ip Assets B.V. | Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars |
| US11339516B2 (en) * | 2018-09-27 | 2022-05-24 | Sanko Tekstil Isletmeleri San. Ve Tic. A.S. | Dyed fabric finishing process |
| CN112912511A (zh) | 2018-10-24 | 2021-06-04 | 帝斯曼知识产权资产管理有限公司 | 用于碳水化合物材料酶促水解和糖发酵的方法 |
| WO2020182843A1 (en) | 2019-03-12 | 2020-09-17 | Dsm Ip Assets B.V. | Process for producing a fermentation broth |
| WO2021048164A1 (en) | 2019-09-10 | 2021-03-18 | Dsm Ip Assets B.V. | Enzyme composition |
| CN111058313A (zh) * | 2020-01-17 | 2020-04-24 | 青岛大学 | 一种提高阳离子颜料碱性条件对棉织物染色深度的方法 |
| WO2022013148A1 (en) | 2020-07-13 | 2022-01-20 | Dsm Ip Assets B.V. | Process for the production of biogas |
| WO2022214459A1 (en) | 2021-04-06 | 2022-10-13 | Dsm Ip Assets B.V. | Enzyme composition |
| CA3213845A1 (en) | 2021-04-06 | 2022-10-13 | Dsm Ip Assets B.V. | Enzyme composition |
| WO2022214458A1 (en) | 2021-04-06 | 2022-10-13 | Dsm Ip Assets B.V. | Enzyme composition |
| BR112023014100A2 (pt) | 2021-04-08 | 2023-10-17 | Versalis Spa | Processo para a preparação de um produto de açúcar e um produto de fermentação |
| CN114317495B (zh) * | 2022-01-10 | 2024-07-30 | 鑫缘茧丝绸集团股份有限公司 | 一种热稳定性提高的葡聚糖酶突变体及其应用 |
| CN115387139A (zh) * | 2022-08-15 | 2022-11-25 | 武汉新华扬生物股份有限公司 | 一种用于布料加工的复配酶制剂及其应用 |
Family Cites Families (87)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1368599A (en) * | 1970-09-29 | 1974-10-02 | Unilever Ltd | Softening compositions |
| JPS5028515B2 (enExample) * | 1971-09-30 | 1975-09-16 | ||
| SU399522A1 (ru) * | 1971-12-30 | 1973-10-03 | натрий карбоксиметил целлюлозе онределение изменению зкости раствора Содержание растворимого белка фильтрах культур, выращенных лабораторных услови течение семи суток жидкой питательной среде свекловичным жомом качестве источника углерода, составл ет, Штамм Ген Штамм | Штамм trichoderma vir1de ген-к9-10-продуцент целлюлолитических ферментов1 |
| US3966543A (en) * | 1972-10-30 | 1976-06-29 | Baxter Laboratories, Inc. | Enzyme-treated paper |
| US4081328A (en) * | 1975-10-23 | 1978-03-28 | Stanford Research Institute | Production of cellulase by a thermophilic thielavia terrestris |
| JPS52134090A (en) * | 1976-04-30 | 1977-11-09 | Ajinomoto Co Inc | Preparation of cellulase |
| DK187280A (da) * | 1980-04-30 | 1981-10-31 | Novo Industri As | Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode |
| GB2094826B (en) | 1981-03-05 | 1985-06-12 | Kao Corp | Cellulase enzyme detergent composition |
| GB2126029A (en) * | 1982-06-11 | 1984-03-14 | Philips Electronic Associated | Producing narrowband SSB-FM signals |
| US4486533A (en) * | 1982-09-02 | 1984-12-04 | St. Louis University | Filamentous fungi functional replicating extrachromosomal element |
| NL8300698A (nl) * | 1983-02-24 | 1984-09-17 | Univ Leiden | Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten. |
| GB8306645D0 (en) * | 1983-03-10 | 1983-04-13 | Unilever Plc | Detergent compositions |
| US4462307A (en) * | 1983-05-23 | 1984-07-31 | Pet Incorporated | Humpback oven-broiler |
| GB8421800D0 (en) * | 1984-08-29 | 1984-10-03 | Unilever Plc | Detergent compositions |
| US4816405A (en) * | 1984-10-24 | 1989-03-28 | The Regents Of The University Of California | Vectors for transformation by ascomycetes |
| US4885249A (en) * | 1984-12-05 | 1989-12-05 | Allelix, Inc. | Aspergillus niger transformation system |
| US4610800A (en) * | 1985-01-25 | 1986-09-09 | Genex Corporation | Method for unclogging drainage pipes |
| US6492107B1 (en) * | 1986-11-20 | 2002-12-10 | Stuart Kauffman | Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique |
| US5198345A (en) * | 1985-04-15 | 1993-03-30 | Gist-Brocades N.V. | Vectors in use in filamentous fungi |
| US5364770A (en) * | 1985-08-29 | 1994-11-15 | Genencor International Inc. | Heterologous polypeptides expressed in aspergillus |
| DK163591C (da) * | 1985-10-08 | 1992-08-24 | Novo Nordisk As | Fremgangsmaade til behandling af et tekstilstof med en cellulase |
| US4935349A (en) * | 1986-01-17 | 1990-06-19 | Zymogenetics, Inc. | Expression of higher eucaryotic genes in aspergillus |
| US5536661A (en) * | 1987-03-10 | 1996-07-16 | Novo Nordisk A/S | Process for the production of protein products in aspergillus |
| US5766912A (en) * | 1986-03-17 | 1998-06-16 | Novo Nordisk A/S | Humicola lipase produced in aspergillus |
| SU1458457A1 (ru) * | 1986-10-16 | 1989-02-15 | Ивановский Химико-Технологический Институт | Способ ферментативной расшлихтовки хлопоксодержащих тканей |
| US5252726A (en) * | 1987-09-04 | 1993-10-12 | Novo Nordisk A/S | Promoters for use in aspergillus |
| US4832864A (en) * | 1987-09-15 | 1989-05-23 | Ecolab Inc. | Compositions and methods that introduce variations in color density into cellulosic fabrics, particularly indigo dyed denim |
| US5223409A (en) * | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| US5122159A (en) * | 1988-09-15 | 1992-06-16 | Ecolab Inc. | Cellulase compositions and methods that introduce variations in color density into cellulosic fabrics, particularly indigo dyed denim |
| US5006126A (en) * | 1988-09-15 | 1991-04-09 | Ecolab Inc. | Cellulase compositions and methods that introduce variations in color density into cellulosic fabrics, particularly indigo dyed denim |
| SU1643608A1 (ru) * | 1989-04-29 | 1991-04-23 | Институт Биохимии Растений Ан Гсср | Штамм гриба АLLеSснеRIа теRRеSтRIS - продуцент целлюлаз |
| US5571697A (en) * | 1989-05-05 | 1996-11-05 | Baylor College Of Medicine Texas Medical Center | Expression of processed recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in Aspergillus |
| WO1991013971A1 (en) * | 1990-03-13 | 1991-09-19 | Hawaii Biotechnology Group, Inc. | Neurospora expression system |
| DK115890D0 (da) * | 1990-05-09 | 1990-05-09 | Novo Nordisk As | Enzym |
| GB9015198D0 (en) * | 1990-07-10 | 1990-08-29 | Brien Caroline J O | Binding substance |
| CA2086092A1 (en) * | 1990-07-13 | 1992-01-14 | Douglas A. Treco | Library screening method |
| JP2859393B2 (ja) * | 1990-07-24 | 1999-02-17 | ノボ ノルディスク アクティーゼルスカブ | セルラーゼ及びその製造法 |
| WO1992002551A1 (en) * | 1990-08-02 | 1992-02-20 | B.R. Centre Limited | Methods for the production of proteins with a desired function |
| JP3133774B2 (ja) * | 1990-10-04 | 2001-02-13 | 日本食品化工株式会社 | 新規な宿主−ベクター系 |
| US5290474A (en) * | 1990-10-05 | 1994-03-01 | Genencor International, Inc. | Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp |
| US5780279A (en) * | 1990-12-03 | 1998-07-14 | Genentech, Inc. | Method of selection of proteolytic cleavage sites by directed evolution and phagemid display |
| DK1225227T3 (da) * | 1990-12-10 | 2009-06-22 | Genencor Int | Forbedret forsukring af cellulose ved kloning og amplifikation af beta-glucosidase genet af trichoderma |
| US5858657A (en) * | 1992-05-15 | 1999-01-12 | Medical Research Council | Methods for producing members of specific binding pairs |
| DE69230142T2 (de) * | 1991-05-15 | 2000-03-09 | Cambridge Antibody Technology Ltd. | Verfahren zur herstellung von spezifischen bindungspaargliedern |
| US5565332A (en) * | 1991-09-23 | 1996-10-15 | Medical Research Council | Production of chimeric antibodies - a combinatorial approach |
| US5516670A (en) * | 1991-09-30 | 1996-05-14 | Kuehnle; Adelheid R. | Magnetophoretic particle delivery method and apparatus for the treatment of cells |
| AU664223B2 (en) * | 1991-12-09 | 1995-11-09 | Unilever Plc | Process for producing/secreting a protein by a transformed mould using expression/secretion regulating regions derived from an (aspergillus) endoxylanase II gene |
| CA2062023A1 (en) * | 1992-02-10 | 1993-08-11 | Jagroop S. Dahiya | Novel fungal strains and use thereof in antibiotic production |
| WO1994005781A1 (en) * | 1992-09-04 | 1994-03-17 | The Scripps Research Institute | Phagemids coexpressing a surface receptor and a surface heterologous protein |
| RU2058135C1 (ru) * | 1993-06-18 | 1996-04-20 | Артюков Александр Алексеевич | Биологическая добавка к моющим средствам для удаления белковых загрязнений |
| CA2166682A1 (en) * | 1993-07-12 | 1995-01-26 | Martin Schulein | A detergent composition comprising two cellulase components |
| GB9316883D0 (en) * | 1993-08-13 | 1993-09-29 | Univ Leeds | Production of heterologous peptices |
| US5849541A (en) * | 1993-11-02 | 1998-12-15 | Merck & Co., Inc. | DNA encoding triol polyketide synthase |
| US5605793A (en) * | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US5820866A (en) * | 1994-03-04 | 1998-10-13 | National Jewish Center For Immunology And Respiratory Medicine | Product and process for T cell regulation |
| ATE256191T1 (de) * | 1994-06-17 | 2003-12-15 | Novozymes As | Ein pilz, in dem das area-gen verändert ist, und ein area-gen aus aspergillus oryzae |
| US6060305A (en) * | 1994-06-30 | 2000-05-09 | Novo Nordisk Biotech, Inc. | Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system |
| WO1996000787A1 (en) * | 1994-06-30 | 1996-01-11 | Novo Nordisk Biotech, Inc. | Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein |
| US5602004A (en) * | 1994-07-20 | 1997-02-11 | Novo Nordisk Biotech, Inc. | Thermophilic fungal expression system |
| US6046021A (en) * | 1995-04-12 | 2000-04-04 | Biolog, Inc. | Comparative phenotype analysis of two or more microorganisms using a plurality of substrates within a multiwell testing device |
| JPH11504218A (ja) * | 1995-04-24 | 1999-04-20 | クロマゾーム コーポレーション | 新規代謝経路の生成およびスクリーニングのための方法 |
| US6030779A (en) * | 1995-07-18 | 2000-02-29 | Diversa Corporation | Screening for novel bioactivities |
| US6057103A (en) * | 1995-07-18 | 2000-05-02 | Diversa Corporation | Screening for novel bioactivities |
| US5958672A (en) * | 1995-07-18 | 1999-09-28 | Diversa Corporation | Protein activity screening of clones having DNA from uncultivated microorganisms |
| BR9611114A (pt) * | 1995-10-17 | 1999-07-13 | Rohm Enzyme Finland Oy | Celulases os genes codificando-as e os usos das mesmas |
| US20030215798A1 (en) * | 1997-06-16 | 2003-11-20 | Diversa Corporation | High throughput fluorescence-based screening for novel enzymes |
| US5939250A (en) * | 1995-12-07 | 1999-08-17 | Diversa Corporation | Production of enzymes having desired activities by mutagenesis |
| US5830696A (en) * | 1996-12-05 | 1998-11-03 | Diversa Corporation | Directed evolution of thermophilic enzymes |
| US5776730A (en) * | 1995-12-15 | 1998-07-07 | University Of Hawaii | Neurospora hosts for the production of recombinant proteins, and methods for producing same |
| EP0877801B1 (en) * | 1996-01-19 | 2005-04-27 | Novozymes Biotech, Inc. | Morphological mutants of filamentous fungi |
| US5753477A (en) * | 1996-03-19 | 1998-05-19 | University Technology Corporation | Magneto-biolistic methods |
| US5783431A (en) * | 1996-04-24 | 1998-07-21 | Chromaxome Corporation | Methods for generating and screening novel metabolic pathways |
| US7883872B2 (en) * | 1996-10-10 | 2011-02-08 | Dyadic International (Usa), Inc. | Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose |
| US5811381A (en) * | 1996-10-10 | 1998-09-22 | Mark A. Emalfarb | Cellulase compositions and methods of use |
| US5879921A (en) * | 1996-11-07 | 1999-03-09 | Novo Nordisk A/S | Recombinant expression of a glucose oxidase from a cladosporium strain |
| WO1998025963A1 (en) * | 1996-12-13 | 1998-06-18 | Chiron Corporation | Analysis and separation of platelet-derived growth factor proteins |
| US5989814A (en) * | 1997-04-01 | 1999-11-23 | Reagents Of The University Of California | Screening methods in eucaryotic cells |
| US6573068B1 (en) * | 1997-11-13 | 2003-06-03 | Genset, S. A. | Claudin-50 protein |
| US6518042B1 (en) * | 1999-02-24 | 2003-02-11 | Novozymes A/S | Process for making DNA libraries in filamentous fungal cells using a novel cloned gene involved in the mismatch repair system of filamentous fungal cells |
| US6121034A (en) * | 1999-05-13 | 2000-09-19 | Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture And Agri-Food | Coniothyrium minitans xylanase gene Cxy1 |
| KR20020026456A (ko) * | 2000-04-13 | 2002-04-10 | 에말파브 마크 아론 | 사상균에서 발현된 ｄｎａ 라이브러리의 고산출량 스크리닝 |
| HUP0300469A2 (hu) * | 2000-04-13 | 2003-06-28 | Emalfarb, Mark Aaron | Új, expressziós szabályzó szekvenciák és expressziós termékek fonalas gombákban |
| WO2002064741A2 (en) * | 2001-02-13 | 2002-08-22 | Diadexus, Inc. | Compositions and methods relating to breast specific genes and proteins |
| CN1980953B (zh) * | 2004-01-30 | 2014-12-24 | 诺维信股份有限公司 | 具有分解纤维增强活性的多肽及其编码多核苷酸 |
| BRPI0507431B1 (pt) * | 2004-02-06 | 2021-07-27 | Novozymes, Inc | Célula hospedeira microbiana recombinante, construto de ácido nucleico, vetor de expressão recombinante, composição detergente, e, métodos para produzir o polipeptídeo gh61, para degradar um material celulósico e para produzir um produto de fermentação |
| US20060105361A1 (en) * | 2004-08-24 | 2006-05-18 | Rothstein Rodney J | Donor yeast strain for transfer of genetic material |
| US7923236B2 (en) * | 2007-08-02 | 2011-04-12 | Dyadic International (Usa), Inc. | Fungal enzymes |
-
1996
- 1996-10-10 US US08/731,170 patent/US5811381A/en not_active Expired - Lifetime
-
1997
- 1997-09-30 CN CNB971996865A patent/CN1177933C/zh not_active Expired - Fee Related
- 1997-09-30 CA CA002268384A patent/CA2268384A1/en not_active Abandoned
- 1997-09-30 DK DK97909913.2T patent/DK0932688T3/da active
- 1997-09-30 US US09/284,152 patent/US7892812B2/en not_active Expired - Fee Related
- 1997-09-30 IL IL12935097A patent/IL129350A0/xx active IP Right Grant
- 1997-09-30 BR BRPI9711876-1B1A patent/BR9711876B1/pt not_active IP Right Cessation
- 1997-09-30 TR TR1999/00771T patent/TR199900771T2/xx unknown
- 1997-09-30 EP EP97909913A patent/EP0932688B1/en not_active Expired - Lifetime
- 1997-09-30 JP JP51759398A patent/JP2001506845A/ja active Pending
- 1997-09-30 AT AT97909913T patent/ATE534733T1/de active
- 1997-09-30 ES ES97909913T patent/ES2377629T3/es not_active Expired - Lifetime
- 1997-09-30 EA EA199900274A patent/EA003295B1/ru not_active IP Right Cessation
- 1997-09-30 AU AU47415/97A patent/AU743909B2/en not_active Ceased
- 1997-09-30 WO PCT/US1997/017669 patent/WO1998015633A1/en not_active Ceased
- 1997-09-30 KR KR1019997003213A patent/KR100641878B1/ko not_active Expired - Fee Related
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1998
- 1998-06-29 US US09/106,026 patent/US6015707A/en not_active Expired - Lifetime
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1999
- 1999-04-06 IL IL129350A patent/IL129350A/en not_active IP Right Cessation
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2008
- 2008-11-05 JP JP2008284868A patent/JP5357514B2/ja not_active Expired - Lifetime
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2011
- 2011-02-21 US US13/031,598 patent/US20110237485A1/en not_active Abandoned
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- 2012-05-15 JP JP2012111665A patent/JP2012147801A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| TR199900771T2 (xx) | 1999-06-21 |
| JP2009065975A (ja) | 2009-04-02 |
| US20030157595A1 (en) | 2003-08-21 |
| ATE534733T1 (de) | 2011-12-15 |
| CN1177933C (zh) | 2004-12-01 |
| KR20000049127A (ko) | 2000-07-25 |
| JP5357514B2 (ja) | 2013-12-04 |
| AU4741597A (en) | 1998-05-05 |
| EA199900274A1 (ru) | 1999-12-29 |
| IL129350A0 (en) | 2000-02-17 |
| US5811381A (en) | 1998-09-22 |
| ES2377629T3 (es) | 2012-03-29 |
| CN1237207A (zh) | 1999-12-01 |
| BR9711876B1 (pt) | 2013-11-12 |
| JP2012147801A (ja) | 2012-08-09 |
| DK0932688T3 (da) | 2012-02-13 |
| WO1998015633A1 (en) | 1998-04-16 |
| EP0932688A1 (en) | 1999-08-04 |
| BR9711876A (pt) | 2000-10-24 |
| US20110237485A1 (en) | 2011-09-29 |
| EP0932688B1 (en) | 2011-11-23 |
| JP2001506845A (ja) | 2001-05-29 |
| KR100641878B1 (ko) | 2006-11-02 |
| IL129350A (en) | 2007-10-31 |
| EA003295B1 (ru) | 2003-04-24 |
| AU743909B2 (en) | 2002-02-07 |
| US6015707A (en) | 2000-01-18 |
| US7892812B2 (en) | 2011-02-22 |
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