BR112012013875A2 - preparações de rna compreendendo rna modificado purificado para reprogramar células - Google Patents

preparações de rna compreendendo rna modificado purificado para reprogramar células

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Publication number
BR112012013875A2
BR112012013875A2 BR112012013875A BR112012013875A BR112012013875A2 BR 112012013875 A2 BR112012013875 A2 BR 112012013875A2 BR 112012013875 A BR112012013875 A BR 112012013875A BR 112012013875 A BR112012013875 A BR 112012013875A BR 112012013875 A2 BR112012013875 A2 BR 112012013875A2
Authority
BR
Brazil
Prior art keywords
rna
somatic cells
preparations
rnas
purified
Prior art date
Application number
BR112012013875A
Other languages
English (en)
Other versions
BR112012013875B8 (pt
BR112012013875B1 (pt
Inventor
Anthony Person
Drew Weissman
Gary Dahl
Jerome Jendrisak
Judith Meis
Katalin Kariko
Original Assignee
Anthony Person
Drew Weissman
Gary Dahl
Jerome Jendrisak
Judith Meis
Katalin Kariko
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anthony Person, Drew Weissman, Gary Dahl, Jerome Jendrisak, Judith Meis, Katalin Kariko filed Critical Anthony Person
Publication of BR112012013875A2 publication Critical patent/BR112012013875A2/pt
Publication of BR112012013875B1 publication Critical patent/BR112012013875B1/pt
Publication of BR112012013875B8 publication Critical patent/BR112012013875B8/pt

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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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Abstract

preparações de rna compreendendo rna modificado purificado para reprogramar células. a presente invenção fornece composições e métodos para reprogramar células somáticas usando preparações de rna purificado compreendendo mrna de fita simples que codifica um fator de indução de célula ips. as preparações de rna purificado são preferencialmente substancialmente isentas de moléculas contaminantes de rna que: i) ativariam uma resposta imune nas células somáticas, ii) diminuiriam a expressão do mrna de fita simples nas céluas somáticas, e/ou iii) ativariam sensores de rna nas células somáticas. em certas modalidades, as preparações de rna purificado são substancialmente isentas de mrnas parciais, rnas de dupla fita, moléculas de rna não capeadas e/ou mrnas de fita simples de passagem.
BR112012013875A 2009-12-07 2010-12-07 método in vitro para reprogramar células somáticas humanas ou de outros mamíferos para células tronco pluripotentes induzidas (células ips ou ipscs) e composição BR112012013875B8 (pt)

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