CY1120237T1 - Παρασκευασματα rna που περιλαμβανουν καθαρισμενο τροποποιημενο rna για επαναπρογραμματισμο κυτταρων - Google Patents
Παρασκευασματα rna που περιλαμβανουν καθαρισμενο τροποποιημενο rna για επαναπρογραμματισμο κυτταρωνInfo
- Publication number
- CY1120237T1 CY1120237T1 CY20181100421T CY181100421T CY1120237T1 CY 1120237 T1 CY1120237 T1 CY 1120237T1 CY 20181100421 T CY20181100421 T CY 20181100421T CY 181100421 T CY181100421 T CY 181100421T CY 1120237 T1 CY1120237 T1 CY 1120237T1
- Authority
- CY
- Cyprus
- Prior art keywords
- rna
- somatic cells
- stranded
- rna preparations
- cell programming
- Prior art date
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- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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- C12Y305/04004—Adenosine deaminase (3.5.4.4)
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Abstract
Η παρούσα εφεύρεση παρέχει συνθέσεις και μεθόδους για επαναπρογραμματισμό σωματικών κυττάρων χρησιμοποιώντας παρασκευάσματα καθαρισμένου RNA που περιλαμβάνουν μονόκλωνο mRNA που κωδικοποιεί παράγοντα διέγερσης κυττάρου iPS. Τα παρασκευάσματα καθαρισμένου RNA κατά προτίμηση ουσιαστικώς είναι ελεύθερα μολυσματικών του RNA μορίων που: i) θα ενεργοποιούν ανοσοαπόκριση στα σωματικά κύτταρα, ii) θα μειώνουν έκφραση του μονόκλωνου mRNA στα σωματικά κύτταρα ή/και iii) ενεργοποιούν RNA αισθητήρες στα σωματικά κύτταρα. Σε ορισμένες πραγματοποιήσεις, τα παρασκευάσματα καθαρισμένου RNA είναι ουσιαστικώς ελεύθερα μερικών mRNAs, διπλόκλων RNAs, μη καλυμμένων RNA μορίων ή/και μονόκλωνων συνεχιζόμενων mRNAs.
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