BR112012010675A2 - maturação ex host de células tronco da linhagem germinativa - Google Patents

maturação ex host de células tronco da linhagem germinativa

Info

Publication number
BR112012010675A2
BR112012010675A2 BR112012010675A BR112012010675A BR112012010675A2 BR 112012010675 A2 BR112012010675 A2 BR 112012010675A2 BR 112012010675 A BR112012010675 A BR 112012010675A BR 112012010675 A BR112012010675 A BR 112012010675A BR 112012010675 A2 BR112012010675 A2 BR 112012010675A2
Authority
BR
Brazil
Prior art keywords
stem cells
germline stem
host maturation
maturation
host
Prior art date
Application number
BR112012010675A
Other languages
English (en)
Inventor
Constance Yuen
Fariborz Izadyar
Johnny Yung-Chiong Chow
Original Assignee
Primegen Biotech Llc Dba Reprocyte
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Primegen Biotech Llc Dba Reprocyte filed Critical Primegen Biotech Llc Dba Reprocyte
Publication of BR112012010675A2 publication Critical patent/BR112012010675A2/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0608Germ cells
    • C12N5/0609Oocytes, oogonia
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00Drugs for genital or sexual disorders; Contraceptives
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00Drugs for genital or sexual disorders; Contraceptives
    • A61P15/08Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0608Germ cells
    • C12N5/061Sperm cells, spermatogonia
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/20Transition metals
    • C12N2500/24Iron; Fe chelators; Transferrin
    • C12N2500/25Insulin-transferrin; Insulin-transferrin-selenium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/70Undefined extracts
    • C12N2500/80Undefined extracts from animals
    • C12N2500/84Undefined extracts from animals from mammals
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/90Serum-free medium, which may still contain naturally-sourced components
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/06Anti-neoplasic drugs, anti-retroviral drugs, e.g. azacytidine, cyclophosphamide
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/11Epidermal growth factor [EGF]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/119Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/125Stem cell factor [SCF], c-kit ligand [KL]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/13Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/15Transforming growth factor beta (TGF-β)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20Cytokines; Chemokines
    • C12N2501/23Interleukins [IL]
    • C12N2501/235Leukemia inhibitory factor [LIF]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30Hormones
    • C12N2501/31Pituitary sex hormones, e.g. follicle-stimulating hormone [FSH], luteinising hormone [LH]; Chorionic gonadotropins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30Hormones
    • C12N2501/38Hormones with nuclear receptors
    • C12N2501/385Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/998Proteins not provided for elsewhere
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00Methods for the dissociation of cells, e.g. specific use of enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/50Proteins
    • C12N2533/54Collagen; Gelatin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/90Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Gynecology & Obstetrics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Pregnancy & Childbirth (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

maturação ex host de células tronco da linhagem germinativa. a presente invenção se refere a métodos que são fornecidos para a maturação ex host de células da linhagem germinativa imaturas em gametas haploides para restauração da fertilidade em pacientes que necessitam dessa restauração.
BR112012010675A 2009-11-05 2010-11-05 maturação ex host de células tronco da linhagem germinativa BR112012010675A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25853509P 2009-11-05 2009-11-05
PCT/US2010/055711 WO2011057128A1 (en) 2009-11-05 2010-11-05 Ex host maturation of germline stem cells

Publications (1)

Publication Number Publication Date
BR112012010675A2 true BR112012010675A2 (pt) 2016-11-29

Family

ID=43478138

Family Applications (2)

Application Number Title Priority Date Filing Date
BR112012010675A BR112012010675A2 (pt) 2009-11-05 2010-11-05 maturação ex host de células tronco da linhagem germinativa
BR112012010750A BR112012010750A2 (pt) 2009-11-05 2010-11-05 sistema de armazenamento de células-tronco germinativas humanas

Family Applications After (1)

Application Number Title Priority Date Filing Date
BR112012010750A BR112012010750A2 (pt) 2009-11-05 2010-11-05 sistema de armazenamento de células-tronco germinativas humanas

Country Status (11)

Country Link
US (2) US20120009156A1 (pt)
EP (2) EP2496939B1 (pt)
JP (2) JP2013514760A (pt)
KR (2) KR20130028041A (pt)
CN (2) CN102713614A (pt)
AU (2) AU2010314989B2 (pt)
BR (2) BR112012010675A2 (pt)
CA (2) CA2779701A1 (pt)
IL (2) IL219601A (pt)
MX (2) MX2012005297A (pt)
WO (2) WO2011057123A1 (pt)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011057123A1 (en) 2009-11-05 2011-05-12 Primegen Biotech, Llc Dba Reprocyte Germline stem cell banking system
CN109486751B (zh) * 2012-02-14 2022-04-26 华盛顿州立大学 用于无饲养细胞培养牛和猪的精原干细胞的方法
EP2886644A1 (en) * 2013-12-20 2015-06-24 Kallistem Process for implementing in vitro spermatogenesis and associated device
EP3006560A1 (en) * 2014-10-09 2016-04-13 European Molecular Biology Laboratory Miwi2 as a marker for spermatogonial stem cells and method for diagnosing spermatogonial stem cells based on said marker
CN104974982B (zh) * 2015-06-19 2018-07-06 中国农业大学 一种体外培养睾丸组织诱导生成精子细胞的方法
CN106754724A (zh) * 2016-12-09 2017-05-31 西北农林科技大学 一种永生化的牛精原干细胞系及其构建方法
KR101986830B1 (ko) * 2017-09-07 2019-06-07 차의과학대학교 산학협력단 줄기세포 유래 세르톨리유사세포, 그 제조방법, 및 그의 용도
EP3700630A4 (en) * 2017-10-27 2021-11-17 Arteriocyte Medical Systems, Inc. INCREASED FERTILITY DUE TO THROMBOCYTE-RICH PLASMA
JP7021828B2 (ja) * 2017-11-10 2022-02-17 一公 川島 卵巣由来幹細胞の減数分裂誘導法
CA3113255A1 (en) * 2018-09-21 2020-03-26 APstem Therapeutics, Inc. Human pluripotent adult stem cells
US20220330884A1 (en) * 2019-09-06 2022-10-20 The Brighan And Women's Hospital, Inc. Automated evaluation of quality assurance metrics for assisted reproduction procedures
AU2022205084A1 (en) * 2021-01-04 2023-07-13 Paterna Biosciences Inc. Process for establishing a human testicular tissue culture system

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6090622A (en) 1997-03-31 2000-07-18 The Johns Hopkins School Of Medicine Human embryonic pluripotent germ cells
AU2002339299A1 (en) * 2001-05-18 2002-12-03 Cornell Research Foundation, Inc. In vitro production of haploid germ cells
CN1174091C (zh) 2002-01-29 2004-11-03 南京医科大学第一附属医院 人成熟卵泡液在未成熟卵体外成熟技术中的应用
US20030215942A1 (en) 2002-02-14 2003-11-20 Stemcyte, Inc. Undesignated allogeneic stem cell bank
US20040091936A1 (en) 2002-05-24 2004-05-13 Michael West Bank of stem cells for producing cells for transplantation having HLA antigens matching those of transplant recipients, and methods for making and using such a stem cell bank
US7087371B2 (en) 2002-06-28 2006-08-08 The Trustees Of The University Of Pennsylvania Material and methods for the production of sperm and analysis thereof
CN1490400A (zh) * 2002-10-17 2004-04-21 中国科学院力学研究所 毛细管微包被法控制细胞空间分布、形状和大小及其应用
CA2548580A1 (en) * 2003-04-03 2004-10-21 Neostem, Inc. Elective collection and banking of autologous peripheral blood stem cells
EP1649038B1 (en) 2003-06-26 2014-07-02 Litron Laboratories Ltd. Method for the enumeration of micronucleated erythrocyte populations while distinguishing platelets and/or platelet-associated aggregates
US20070011756A1 (en) 2003-08-27 2007-01-11 Takashi Shinohara Method of constructing offspring originating from frozen sperm stem cell
CA2561690A1 (en) 2004-03-30 2005-10-27 Kyoto University Process for producing multipotential stem cell originating in testoid cell
EP1735430A4 (en) * 2004-04-13 2007-10-31 Sungkwang Educational Foundati IN VITRO METHOD FOR ISOLATION, PROLIFERATION AND DIFFERENTIATION OF GERMPLE STEM CELLS
US7955846B2 (en) 2004-05-17 2011-06-07 The General Hospital Corporation Compositions comprising female germline stem cells and methods of use thereof
US20070020759A1 (en) 2004-07-15 2007-01-25 Primegen Biotech Llc Therapeutic reprogramming of germ line stem cells
DK1789534T3 (en) 2004-08-16 2014-11-17 Cellres Corp Pte Ltd ISOLATION OF stem / progenitor cells FOSTER HINDE FROM an umbilical cord
JP2008541734A (ja) * 2005-05-30 2008-11-27 コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガニゼイション 基底膜粒子の製造及び使用
US7732202B2 (en) * 2005-10-21 2010-06-08 International Stem Cell Corporation Oxygen tension for the parthenogenic activation of human oocytes for the production of human embryonic stem cells
EP1943335A2 (en) 2005-11-02 2008-07-16 Georg-August-Universität Göttingen Compositions and methods for producing pluripotent cells from adult testis
US20090170059A1 (en) 2005-11-14 2009-07-02 Hans Klingemann Methods for Preparing Cord Matrix Stem Cells (CMSC) for Long Term Storage and for Preparing a Segment of umbilical cord for cryopreservation
CN1800371A (zh) 2005-12-19 2006-07-12 浙江大学 一种人体未成熟卵体外成熟培养方法
KR100707985B1 (ko) 2006-03-10 2007-04-16 세원셀론텍(주) 세포치료제를 생산 및 보관하기 위한 모듈 장치
KR100745362B1 (ko) 2006-03-14 2007-08-02 세원셀론텍(주) 세포치료제 설비의 사용방법 및 이를 이용한 네트워크기반의 프랜차이즈 마켓 비즈니스 방법
WO2007115216A1 (en) 2006-03-30 2007-10-11 Primegen Biotech Llc Reprogramming of adult human testicular stem cells to pluripotent germ-line stem cells
US8759090B2 (en) 2006-10-30 2014-06-24 University Of Central Florida Research Foundation, Inc. Stem cell banking system
US20080132803A1 (en) 2006-11-30 2008-06-05 Hyman Friedlander Method and system for doing business by mining the placental-chord complex
CN100999722B (zh) 2007-01-08 2011-08-03 安徽省立医院 卵巢组织块内不成熟卵母细胞的体外培养成熟方法
US20080189045A1 (en) 2007-02-06 2008-08-07 Moore Thomas E Method for the Collection And Distribution of Cord Blood Stem Cells
CN100548313C (zh) 2007-06-05 2009-10-14 南京农业大学 细管高密度冷冻猪精液的方法及其产品
MX2010001528A (es) * 2007-08-07 2010-10-15 Primegen Biotech Llc Aislamiento, caracterizacion y propagacion de celulas madre de linea germinal.
US20090138354A1 (en) 2007-11-25 2009-05-28 Herbert Zech Methods of Storing and Providing Samples of Cells and Tissue
US8673637B2 (en) * 2008-10-16 2014-03-18 The Board Of Trustees Of The Leland Stanford Junior University Human multipotent germ line stem cells expressing a germ line marker and a pluripotency marker produced by co-culture of testicular tissue
CN101429492B (zh) 2008-12-12 2010-10-06 深圳华大基因科技有限公司 一种猪卵母细胞体外培养成熟方法
WO2011057123A1 (en) 2009-11-05 2011-05-12 Primegen Biotech, Llc Dba Reprocyte Germline stem cell banking system

Also Published As

Publication number Publication date
JP2013514760A (ja) 2013-05-02
KR20120120157A (ko) 2012-11-01
BR112012010750A2 (pt) 2019-09-24
US20120083034A1 (en) 2012-04-05
AU2010314984B2 (en) 2015-09-17
KR20130028041A (ko) 2013-03-18
WO2011057123A8 (en) 2012-06-07
MX344716B (es) 2017-01-03
AU2010314984A8 (en) 2014-02-27
CN102713614A (zh) 2012-10-03
EP2496940B1 (en) 2018-08-15
EP2496939B1 (en) 2017-02-15
CA2779726C (en) 2018-03-06
IL219601A (en) 2017-05-29
CN103154730B (zh) 2015-04-29
AU2010314989A1 (en) 2012-05-24
CA2779701A1 (en) 2011-05-12
WO2011057123A1 (en) 2011-05-12
MX2012005297A (es) 2013-02-26
EP2496940A1 (en) 2012-09-12
CA2779726A1 (en) 2011-05-12
WO2011057128A1 (en) 2011-05-12
AU2010314984A1 (en) 2012-05-24
US9657267B2 (en) 2017-05-23
CN103154730A (zh) 2013-06-12
MX2012005298A (es) 2012-12-17
IL219600A0 (en) 2012-06-28
EP2496939A1 (en) 2012-09-12
US20120009156A1 (en) 2012-01-12
JP2013514969A (ja) 2013-05-02
IL219601A0 (en) 2012-07-31
AU2010314989B2 (en) 2013-10-24

Similar Documents

Publication Publication Date Title
BR112012010675A2 (pt) maturação ex host de células tronco da linhagem germinativa
CL2012000590A1 (es) Proceso para preparar w-transaminasa (r)-selectiva.
MX2022008149A (es) Replica de carne que comprende un indicador del progreso de coccion.
MX2013001045A (es) Organoide hepatico, sus usos y metodo de cultivo para obtenerlos.
UY33958A (es) Inhibidores de la glucosilceramida sintasa
MX2013008850A (es) Composiciones y metodos para tratar enfermedades cardiovasculares.
CR20130613A (es) Herramienta dental y dispositivos de guía
CR20120184A (es) Anticuerpos monoclonales frente a progastrina y su usos
CO6561839A2 (es) Alteracion genómica dirigida
MX344786B (es) Composiciones y metodos para tratar enfermedad de gaucher.
CR20120485A (es) Procedimientos para la producción de complejos de ciclodextrina para formular inhibidores del proteasoma peptídico
IN2014DN08481A (pt)
BR112012008136A2 (pt) Composição nutritiva para mulheres que amamentam
BR112013024423A2 (pt) detecção de doença em plantas
BR112013029498A2 (pt) “método para produzir um suíno geneticamente modificado.”
TR201909271T4 (tr) Testosteron formülasyonları.
AU2013225816A8 (en) Compositions and methods for treating Type III Gaucher Disease
UY33710A (es) Marcadores de soja relacionados con resistencia al scn
MY167208A (en) Partially Hydrolyzed Casein-Whey Nutritional Compositions for Reducing the Onset of Allergies
ECSP11011486A (es) Producción de hidrocarburos de isopreno mejorada que usa citanobacterias diseñadas geneticamente
CO7151478A2 (es) Formulaciones estabilizadas que contienen anticuerpos anti-dll4
EP2435558A4 (en) PRODUCTION OF HEAVY INDUCED PLURIPOTENT STEM CELLS GENERALLY CORRECTED
CR20120381A (es) Composiciones nutricionales que comprenden hojuelas de frutas que incluyen acido docosahexaenoico
EP2571497A4 (en) METHODS AND COMPOSITIONS RELATING TO THE SELECTIVE CHANNEL OF RETINOID RECEPTORS
UY34378A (es) Proceso para producir peróxido de hidrógeno

Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]
B08G Application fees: restoration [chapter 8.7 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]
B08G Application fees: restoration [chapter 8.7 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06T Formal requirements before examination [chapter 6.20 patent gazette]
B11E Dismissal acc. art. 34 of ipl - requirements for examination incomplete
B11T Dismissal of application maintained [chapter 11.20 patent gazette]