MX2010001528A - Aislamiento, caracterizacion y propagacion de celulas madre de linea germinal. - Google Patents

Aislamiento, caracterizacion y propagacion de celulas madre de linea germinal.

Info

Publication number
MX2010001528A
MX2010001528A MX2010001528A MX2010001528A MX2010001528A MX 2010001528 A MX2010001528 A MX 2010001528A MX 2010001528 A MX2010001528 A MX 2010001528A MX 2010001528 A MX2010001528 A MX 2010001528A MX 2010001528 A MX2010001528 A MX 2010001528A
Authority
MX
Mexico
Prior art keywords
cells
characterization
isolation
stem cells
germline
Prior art date
Application number
MX2010001528A
Other languages
English (en)
Inventor
Fariborz Izadyar
Jason Pacchiarotti
Chad Maki
Thomas V Romas
Original Assignee
Primegen Biotech Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Primegen Biotech Llc filed Critical Primegen Biotech Llc
Publication of MX2010001528A publication Critical patent/MX2010001528A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0611—Primordial germ cells, e.g. embryonic germ cells [EG]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0609—Oocytes, oogonia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/13—Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Se proporcionan métodos para el aislamiento, caracterización y propagación de células madre de células de línea germinal de mamíferos fetales y adultos. Además, se escriben poblaciones aisladas de células de línea germinal teniendo diferentes fenotipos, en donde las sub-poblaciones son capaces de formar cultivos a largo plazo de células multipotentes o pluripotentes o son capaces de diferenciar a células de línea germinal maduras y re-poblar un órgano reproductivo estéril. Las células de línea germinal multipotentes y pluripotentes también son adecuadas para diferenciación a células somáticas de tejido específico para propósitos terapéuticos.
MX2010001528A 2007-08-07 2008-08-07 Aislamiento, caracterizacion y propagacion de celulas madre de linea germinal. MX2010001528A (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US95449607P 2007-08-07 2007-08-07
US2650208P 2008-02-06 2008-02-06
US6035608P 2008-06-10 2008-06-10
PCT/US2008/072541 WO2009021151A1 (en) 2007-08-07 2008-08-07 Isolation, characterization and propagation of germline stem cells

Publications (1)

Publication Number Publication Date
MX2010001528A true MX2010001528A (es) 2010-10-15

Family

ID=39832540

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2010001528A MX2010001528A (es) 2007-08-07 2008-08-07 Aislamiento, caracterizacion y propagacion de celulas madre de linea germinal.

Country Status (6)

Country Link
US (1) US20100285577A1 (es)
EP (2) EP2176399A1 (es)
KR (1) KR20100093513A (es)
CN (1) CN102105579A (es)
MX (1) MX2010001528A (es)
WO (1) WO2009021151A1 (es)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2294185A2 (en) * 2008-05-08 2011-03-16 Coretherapix Slu Multipotent adult stem cell population
BR112012010675A2 (pt) * 2009-11-05 2016-11-29 Primegen Biotech Llc Dba Reprocyte maturação ex host de células tronco da linhagem germinativa
EP3409112B1 (en) * 2011-05-04 2022-07-27 The University of Nottingham Novel polymers which resist bacterial attachment
CN102250830B (zh) * 2011-07-05 2013-01-16 西北农林科技大学 一种雌性生殖干细胞的体外分离及培养方法
CN103087979A (zh) * 2011-10-28 2013-05-08 李福生 用于富集、提纯干细胞的方法、试剂盒及其应用方法
CN109486751B (zh) * 2012-02-14 2022-04-26 华盛顿州立大学 用于无饲养细胞培养牛和猪的精原干细胞的方法
CN103305459A (zh) * 2012-03-16 2013-09-18 南开大学 小分子诱导猪精原干细胞转变为胚胎干细胞样细胞的方法
JP6362311B2 (ja) * 2012-09-06 2018-07-25 オリンパス株式会社 積層構造体中の細胞の画像解析方法、及び角膜移植用積層構造体の評価方法
CN102824226B (zh) * 2012-09-14 2015-10-21 中国农业科学院北京畜牧兽医研究所 一种精原干细胞移植受体的制备方法
EP3160385A4 (en) * 2014-06-30 2019-04-10 Primegen Biotech LLC STEM CELLS OF SUB-POPULATIONS DERIVED FROM GONADES
EP3245287A4 (en) * 2015-01-13 2018-06-20 President and Fellows of Harvard College Purification of germ stem cells by targeting mrp9
CN108369734B (zh) * 2015-12-10 2022-04-26 凯杰有限公司 数字图像中的对象分类方法、系统及计算机可读介质
CN108603168B (zh) 2016-02-04 2021-11-09 上海交通大学 从卵泡抽提物中获得雌性生殖干细胞的方法
CN106754724A (zh) * 2016-12-09 2017-05-31 西北农林科技大学 一种永生化的牛精原干细胞系及其构建方法
CN110777109B (zh) * 2019-12-02 2021-08-31 浙江省海洋水产养殖研究所 泥蚶性腺组织生殖细胞分离与培养的方法
CA3207158A1 (en) * 2021-01-04 2022-07-07 Paterna Biosciences Inc. Process for establishing a human testicular tissue culture system
CN114276984B (zh) * 2021-12-31 2024-01-16 上海交通大学 雌性生殖干细胞转分化至功能精子的方法及应用
CN114457015A (zh) * 2022-03-11 2022-05-10 中国科学院广州生物医药与健康研究院 纹状体类脑器官及其培养基、培养方法和应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6090622A (en) * 1997-03-31 2000-07-18 The Johns Hopkins School Of Medicine Human embryonic pluripotent germ cells
WO2005100548A1 (ja) * 2004-03-30 2005-10-27 Kyoto University 精巣細胞由来多能性幹細胞の製造方法
US7955846B2 (en) * 2004-05-17 2011-06-07 The General Hospital Corporation Compositions comprising female germline stem cells and methods of use thereof
AU2005252636A1 (en) * 2004-05-17 2005-12-22 The General Hospital Corporation Compositions comprising female germline stem cells and methods of use thereof
US20070020759A1 (en) * 2004-07-15 2007-01-25 Primegen Biotech Llc Therapeutic reprogramming of germ line stem cells
US8232077B2 (en) * 2005-07-06 2012-07-31 Ovacyte Llc Oocytes derived from ovarian culture initially containing no oocytes
WO2007051625A2 (en) * 2005-11-02 2007-05-10 Georg-August-Universität Göttingen Compositions and methods for producing pluripotent cells from adult testis
WO2007115216A1 (en) * 2006-03-30 2007-10-11 Primegen Biotech Llc Reprogramming of adult human testicular stem cells to pluripotent germ-line stem cells

Also Published As

Publication number Publication date
WO2009021151A1 (en) 2009-02-12
EP2527426A1 (en) 2012-11-28
CN102105579A (zh) 2011-06-22
WO2009021151A8 (en) 2010-03-18
KR20100093513A (ko) 2010-08-25
EP2176399A1 (en) 2010-04-21
US20100285577A1 (en) 2010-11-11

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