AU2018215335B2 - Engineered glycosyltransferases and steviol glycoside glucosylation methods - Google Patents

Engineered glycosyltransferases and steviol glycoside glucosylation methods Download PDF

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AU2018215335B2
AU2018215335B2 AU2018215335A AU2018215335A AU2018215335B2 AU 2018215335 B2 AU2018215335 B2 AU 2018215335B2 AU 2018215335 A AU2018215335 A AU 2018215335A AU 2018215335 A AU2018215335 A AU 2018215335A AU 2018215335 B2 AU2018215335 B2 AU 2018215335B2
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seq
mutation
positions
engineered
polypeptide sequence
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AU2018215335A1 (en
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Nikki DELLAS
David Entwistle
Stephanie Sue Galanie
Jack Liang
Joyce Liu
Courtney Dianne Moffett
Jonathan VROOM
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Tate and Lyle Solutions USA LLC
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Priority to AU2022268390A priority patent/AU2022268390B2/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1062—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01013—Sucrose synthase (2.4.1.13)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
AU2018215335A 2017-02-03 2018-02-01 Engineered glycosyltransferases and steviol glycoside glucosylation methods Active AU2018215335B2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2020289865A AU2020289865B2 (en) 2017-02-03 2020-12-18 Engineered glycosyltransferases and steviol glycoside glucosylation methods
AU2020289868A AU2020289868B2 (en) 2017-02-03 2020-12-18 Engineered glycosyltransferases and steviol glycoside glucosylation methods
AU2022268390A AU2022268390B2 (en) 2017-02-03 2022-11-11 Engineered glycosyltransferases and steviol glycoside glucosylation methods

Applications Claiming Priority (5)

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US201762454417P 2017-02-03 2017-02-03
US62/454,417 2017-02-03
US201762479262P 2017-03-30 2017-03-30
US62/479,262 2017-03-30
PCT/US2018/016359 WO2018144679A2 (en) 2017-02-03 2018-02-01 Engineered glycosyltransferases and steviol glycoside glucosylation methods

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AU2020289865A Division AU2020289865B2 (en) 2017-02-03 2020-12-18 Engineered glycosyltransferases and steviol glycoside glucosylation methods

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AU2020289865A Active AU2020289865B2 (en) 2017-02-03 2020-12-18 Engineered glycosyltransferases and steviol glycoside glucosylation methods
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AU2022268390A Active AU2022268390B2 (en) 2017-02-03 2022-11-11 Engineered glycosyltransferases and steviol glycoside glucosylation methods

Country Status (15)

Country Link
US (5) US20180223264A1 (https=)
EP (3) EP3577229A4 (https=)
JP (4) JP7248302B2 (https=)
KR (3) KR102905512B1 (https=)
CN (2) CN110914445B (https=)
AU (4) AU2018215335B2 (https=)
BR (1) BR112019015992A2 (https=)
CA (3) CA3175100A1 (https=)
IL (3) IL304833B2 (https=)
MX (4) MX391104B (https=)
MY (1) MY199186A (https=)
PH (1) PH12019501698A1 (https=)
SG (1) SG11201906480QA (https=)
TW (3) TW202237833A (https=)
WO (2) WO2018144675A1 (https=)

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CN114480326B (zh) * 2021-01-08 2024-04-26 中国科学院华南植物园 亚精胺衍生物糖基转移酶LbUGT73及其编码基因和应用
CN112704731B (zh) * 2021-01-13 2023-08-25 中国农业科学院哈尔滨兽医研究所（中国动物卫生与流行病学中心哈尔滨分中心） 蛋白酶s273r抑制细胞焦亡的用途及方法
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EP4724575A1 (en) * 2023-06-06 2026-04-15 Arzeda Corp. Compositions and methods for producing rebaudioside d and rebaudioside m
CN119432785B (zh) * 2023-08-07 2026-02-06 中国科学院天津工业生物技术研究所 一种高丝氨酸脱氢酶突变体及其应用
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CN119931981A (zh) * 2024-05-29 2025-05-06 东台市浩瑞生物科技有限公司 一种糖基转移酶ugt76g1突变体及其催化合成莱鲍迪苷a的方法
WO2026063396A1 (ja) * 2024-09-20 2026-03-26 富士フイルム株式会社 変異型スクロースシンターゼ、変異型スクロースシンターゼをコードする核酸、発現ベクター、形質転換体、変異型スクロースシンターゼの製造方法、変異型スクロースシンターゼを含む組成物、ウリジン二リン酸－グルコースの製造方法、糖化合物の製造方法
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