AU2016262494B2 - Expression constructs and methods of genetically engineering methylotrophic yeast - Google Patents

Expression constructs and methods of genetically engineering methylotrophic yeast Download PDF

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Publication number
AU2016262494B2
AU2016262494B2 AU2016262494A AU2016262494A AU2016262494B2 AU 2016262494 B2 AU2016262494 B2 AU 2016262494B2 AU 2016262494 A AU2016262494 A AU 2016262494A AU 2016262494 A AU2016262494 A AU 2016262494A AU 2016262494 B2 AU2016262494 B2 AU 2016262494B2
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nucleic acid
yeast cell
promoter element
methanol
cell
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AU2016262494A1 (en
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Martin Andrew HOYT
Smita SHANKAR
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Impossible Foods Inc
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Impossible Foods Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/805—Haemoglobins; Myoglobins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/84—Pichia
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
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  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
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  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2016262494A 2015-05-11 2016-05-11 Expression constructs and methods of genetically engineering methylotrophic yeast Active AU2016262494B2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2019250216A AU2019250216B2 (en) 2015-05-11 2019-10-17 Expression constructs and methods of genetically engineering methylotrophic yeast
AU2022201465A AU2022201465C1 (en) 2015-05-11 2022-03-03 Expression constructs and methods of genetically engineering methylotrophic yeast
AU2025201786A AU2025201786A1 (en) 2015-05-11 2025-03-12 Expression constructs and methods of genetically engineering methylotrophic yeast

Applications Claiming Priority (17)

Application Number Priority Date Filing Date Title
US201562159899P 2015-05-11 2015-05-11
US62/159,899 2015-05-11
US201562183074P 2015-06-22 2015-06-22
US62/183,074 2015-06-22
US201562185921P 2015-06-29 2015-06-29
US62/185,921 2015-06-29
US201562203052P 2015-08-10 2015-08-10
US62/203,052 2015-08-10
US201562220366P 2015-09-18 2015-09-18
US62/220,366 2015-09-18
US201562222388P 2015-09-23 2015-09-23
US62/222,388 2015-09-23
US201562236506P 2015-10-02 2015-10-02
US62/236,506 2015-10-02
US201662313491P 2016-03-25 2016-03-25
US62/313,491 2016-03-25
PCT/US2016/031797 WO2016183163A1 (en) 2015-05-11 2016-05-11 Expression constructs and methods of genetically engineering methylotrophic yeast

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AU2019250216A Division AU2019250216B2 (en) 2015-05-11 2019-10-17 Expression constructs and methods of genetically engineering methylotrophic yeast

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AU2016262494B2 true AU2016262494B2 (en) 2019-11-07

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AU2019250216A Active AU2019250216B2 (en) 2015-05-11 2019-10-17 Expression constructs and methods of genetically engineering methylotrophic yeast
AU2022201465A Active AU2022201465C1 (en) 2015-05-11 2022-03-03 Expression constructs and methods of genetically engineering methylotrophic yeast
AU2025201786A Pending AU2025201786A1 (en) 2015-05-11 2025-03-12 Expression constructs and methods of genetically engineering methylotrophic yeast

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AU2022201465A Active AU2022201465C1 (en) 2015-05-11 2022-03-03 Expression constructs and methods of genetically engineering methylotrophic yeast
AU2025201786A Pending AU2025201786A1 (en) 2015-05-11 2025-03-12 Expression constructs and methods of genetically engineering methylotrophic yeast

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US (6) US10689656B2 (https=)
EP (3) EP4624565A3 (https=)
JP (5) JP6831797B2 (https=)
KR (4) KR20250048145A (https=)
CN (1) CN107614516A (https=)
AU (4) AU2016262494B2 (https=)
CA (1) CA2985641A1 (https=)
CY (1) CY1125979T1 (https=)
DK (1) DK3294762T3 (https=)
ES (2) ES3051659T3 (https=)
HR (1) HRP20220299T1 (https=)
HU (1) HUE058104T2 (https=)
LT (1) LT3294762T (https=)
MX (3) MX2017014469A (https=)
NZ (1) NZ737094A (https=)
PL (1) PL3294762T3 (https=)
PT (1) PT3294762T (https=)
RS (1) RS63099B1 (https=)
SI (1) SI3294762T1 (https=)
SM (1) SMT202200124T1 (https=)
WO (1) WO2016183163A1 (https=)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT3294762T (pt) 2015-05-11 2022-03-21 Impossible Foods Inc Constructos de expressão e métodos de modificar geneticamente levedura metilotrófica
US10988515B2 (en) 2017-01-13 2021-04-27 Bolt Threads, Inc. Elastomeric proteins
WO2018140654A1 (en) * 2017-01-25 2018-08-02 Altius Institute For Biomedical Sciences Methods and platform of designing genetic editing tools
CA3076249A1 (en) 2017-09-21 2019-03-28 The Brigham And Women's Hospital, Inc. Isolation, preservation, compositions and uses of extracts from justicia plants
US11051532B2 (en) 2017-09-22 2021-07-06 Impossible Foods Inc. Methods for purifying protein
US10221403B1 (en) * 2018-04-25 2019-03-05 Life Rainbow Biotech Co., Ltd. Method of preparing zearalenone hydrolase
CN108913732B (zh) * 2018-07-31 2022-02-08 华东理工大学 一种莫纳可林j异源生产的方法及应用
CN111286520B (zh) * 2018-12-10 2021-05-07 上海凯赛生物技术股份有限公司 用于发酵生产l-赖氨酸的重组dna、菌株及其应用
CN109929769B (zh) * 2019-03-05 2021-07-23 天津科技大学 一种利用fld1启动子调控黏玉米谷氨酰胺转氨酶表达的重组毕赤酵母菌株及构建方法
CN109749948A (zh) * 2019-03-05 2019-05-14 天津科技大学 一种利用tef1启动子调控黏玉米谷氨酰胺转氨酶表达的重组毕赤酵母菌株及构建方法
AU2020257270B2 (en) 2019-04-17 2026-01-15 Impossible Foods Inc. Materials and methods for protein production
EP3959311A1 (en) 2019-04-25 2022-03-02 Impossible Foods Inc. Strains and methods for production of heme-containing proteins
WO2021108502A1 (en) 2019-11-27 2021-06-03 Evelo Biosciences, Inc. Methods and compositions for culturing hemoglobin-dependent bacteria
KR20220123019A (ko) 2019-12-31 2022-09-05 에어 프로틴 인코포레이티드 고단백질 식품 조성물
EP4090746A4 (en) * 2020-01-10 2024-01-24 Intron Biotechnology, Inc. A method for preparing soy leghemoglobin using escherichia coli
AU2021229443A1 (en) 2020-03-02 2022-09-15 Air Protein, Inc. Structured high-protein meat analogue compositions
US12011016B2 (en) 2020-09-14 2024-06-18 Impossible Foods Inc. Protein methods and compositions
US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants
AU2021353004A1 (en) 2020-09-30 2023-04-13 Nobell Foods, Inc. Recombinant milk proteins and food compositions comprising the same
US10894812B1 (en) 2020-09-30 2021-01-19 Alpine Roads, Inc. Recombinant milk proteins
WO2022072833A2 (en) 2020-10-02 2022-04-07 Impossible Foods Inc. Expression constructs and methods of genetically engineering cells
WO2022072846A2 (en) 2020-10-02 2022-04-07 Impossible Foods Inc. Transgenic plants with altered fatty acid profiles and upregulated heme biosynthesis
WO2022117743A1 (en) * 2020-12-02 2022-06-09 Genzyme Corporation Polypeptide having beta-hexosaminidase activity, and polynucleotides coding for the same
JP2023553042A (ja) * 2020-12-02 2023-12-20 プランイート フーズ プライベート リミテッド 組換えによりグロビンポリペプチドを生成する方法および肉代替食品製品
WO2022251166A2 (en) 2021-05-25 2022-12-01 Evelo Biosciences, Inc. Bacterial compositions comprising soy hemoglobin
AU2022369299A1 (en) 2021-10-19 2024-03-14 Eat Scifi Inc. Plant base/animal cell hybrid meat substitute
KR20240118861A (ko) * 2021-12-16 2024-08-05 임파서블 푸즈 인크. 재조합 단백질의 발현을 증가시키는 유전 인자
US11931687B2 (en) 2022-02-15 2024-03-19 Streamline Innovations, Inc. Gas purification apparatus and method
KR102804540B1 (ko) 2022-05-25 2025-05-07 한화솔루션 주식회사 야생형 효모 균주를 이용한 헴 고생산 방법
WO2024022474A1 (zh) 2022-07-29 2024-02-01 南京百斯杰生物工程有限公司 一种嵌合型转录激活因子及其应用
KR20240098834A (ko) 2022-12-21 2024-06-28 한화솔루션 주식회사 고농도의 헴을 생산하는 효모 돌연변이 균주 및 이를 이용한 헴 고생산 방법
CN116042621A (zh) * 2023-01-06 2023-05-02 江南大学 一种酵母杂交启动子及其应用
WO2024167695A1 (en) 2023-02-08 2024-08-15 Danisco Us Inc. Compositions and methods for producing heterologous globins in filamentous fungal cells
CN116410996A (zh) * 2023-02-15 2023-07-11 浙江大学 一种血红蛋白的编码基因表达盒及其应用
KR20240162935A (ko) 2023-05-09 2024-11-18 한화솔루션 주식회사 고농도의 헴을 생산하는 효모 돌연변이 균주 hs-y010 및 이를 이용한 헴 고생산 방법
KR20240162936A (ko) 2023-05-09 2024-11-18 한화솔루션 주식회사 고농도의 헴을 생산하는 효모 돌연변이 균주 hs-y007 및 이를 이용한 헴 고생산 방법
EP4726051A1 (en) * 2023-06-06 2026-04-15 FUJIFILM Corporation Recombinant yeast, method for producing target protein, and kit and method for producing recombinant yeast
WO2025247966A1 (en) * 2024-05-29 2025-12-04 Mybios Gmbh Process for protein production in methylotrophic yeast host cell.
WO2025259480A2 (en) 2024-06-12 2025-12-18 Streamline Innovations Inc Gas purification apparatus with eductor reduction unit and method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014008729A1 (zh) * 2012-07-12 2014-01-16 华东理工大学 消除甲醇诱导型启动子单一甲醇碳源依赖性的方法

Family Cites Families (71)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4158065A (en) 1977-12-16 1979-06-12 Sugiyo Co., Ltd. Crab meat substitute and method of preparing same
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DK162399C (da) 1986-01-28 1992-03-23 Danisco Fremgangsmaade til ekspression af gener i baelgplanteceller, dna-fragment, rekombineret dna-fragment samt plasmid til brug ved udoevelsen af fremgangsmaaden
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5663143A (en) 1988-09-02 1997-09-02 Dyax Corp. Engineered human-derived kunitz domains that inhibit human neutrophil elastase
US6946587B1 (en) 1990-01-22 2005-09-20 Dekalb Genetics Corporation Method for preparing fertile transgenic corn plants
US5204253A (en) 1990-05-29 1993-04-20 E. I. Du Pont De Nemours And Company Method and apparatus for introducing biological substances into living cells
US5753465A (en) 1994-08-30 1998-05-19 Carnegie Mellon University Unmodified recombinant human adult hemoglobin production
US5538800A (en) 1994-09-29 1996-07-23 At&T Corp. Magnetoresistive oxide material and articles comprising the material
FR2736930B1 (fr) 1995-07-17 1997-09-19 Biocem Procede de production, par des cellules vegetales, de proteines heminiques, proteines ainsi obtenues et produits contenant ces proteines
US5824511A (en) 1995-08-01 1998-10-20 University Technology Corporation Method for enhancing the production of hemoproteins
PL328096A1 (en) 1996-02-01 1999-01-04 North American Vaccine Expression of external membrane (mb3) protein of neisseria meningitidis of b group in yeasts and vaccines
JP3462218B2 (ja) 1996-06-10 2003-11-05 ノボザイムス バイオテック，インコーポレイティド 糸状菌におけるヘムタンパク質生産を増加せしめるための方法
JP3794760B2 (ja) 1996-07-30 2006-07-12 三井化学株式会社 フィターゼ生産酵母
US5959187A (en) 1996-09-26 1999-09-28 Bailey; James E. Expression of oxygen-binding proteins in plants
JPH10117776A (ja) 1996-10-22 1998-05-12 Japan Tobacco Inc インディカイネの形質転換方法
CA2331772C (en) 1998-06-26 2009-08-04 The University Of Manitoba Hemoglobins to maintain cell energy status
US7803765B2 (en) 1999-05-05 2010-09-28 Phylogica Limited Methods of constructing biodiverse gene fragment libraries and biological modulators isolated therefrom
US7498304B2 (en) 2000-06-16 2009-03-03 Curis, Inc. Angiogenesis-modulating compositions and uses
WO2001098480A2 (en) 2000-06-23 2001-12-27 Syngenta Participations Ag Promoters for regulation of plant gene expression
EP1356060B1 (en) 2000-09-18 2005-12-14 Genencor International, Inc. Twin-arginine translocation in bacillus
US8021695B2 (en) 2002-02-15 2011-09-20 Arch Personal Care Products, L.P. Personal care composition containing leghemoglobin
AU2003296714A1 (en) 2002-12-23 2004-07-22 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften Ev Method for altering the content of reserve substances in plants
WO2004099405A1 (en) 2003-04-11 2004-11-18 Ventria Bioscience Human blood proteins expressed in monocot seeds
KR100660539B1 (ko) 2004-07-29 2006-12-22 삼성전자주식회사 자기 기억 소자 및 그 형성 방법
CN1308452C (zh) 2004-09-14 2007-04-04 上海永业农科生物工程有限公司 一种含有改组乙醇氧化酶启动子的毕赤酵母植酸酶表达系统
US20080193969A1 (en) 2005-01-12 2008-08-14 Rob Te Biesebeke Hemoglobin Overexpression in Fungal Fermentations
AT501955B1 (de) 2005-02-23 2007-08-15 Univ Graz Tech Mutierte aox1-promotoren
EP2479254A1 (en) 2005-08-24 2012-07-25 The Scripps Research Institute Translation enhancer-element dependent vector systems
JP2007228956A (ja) 2006-02-28 2007-09-13 Suntory Ltd 醸造用酵母由来の有用タンパク質同定方法
JP2008017733A (ja) 2006-07-11 2008-01-31 Tokyo Univ Of Agriculture 異種タンパク質の発現方法及び異種タンパク質の発現方法により得られたタンパク質
JP2008017774A (ja) * 2006-07-13 2008-01-31 Nipro Corp ピキア酵母を用いた組換えヒトヘモグロビンの製造
CN101541187A (zh) 2006-11-01 2009-09-23 西格马食品可变资本有限公司 肉类替代食品及其制备方法
US8143023B2 (en) 2007-01-26 2012-03-27 Novozymes A/S Method for methanol independent induction from methanol inducible promoters in Pichia
WO2009009142A2 (en) 2007-07-10 2009-01-15 Monsanto Technology, Llc Transgenic plants with enhanced agronomic traits
EP2058398A1 (en) * 2007-11-09 2009-05-13 Nipro Corporation Production of recombinant human hemoglobin using pichia yeast
CN102105589A (zh) 2008-07-11 2011-06-22 诺维信公司 巴斯德毕赤酵母(Pichia pastoris) DAS启动子变体
US20120156761A1 (en) 2009-07-02 2012-06-21 Verdezyne, Inc. Biological methods for preparing adipic acid
US20120184007A1 (en) 2009-07-09 2012-07-19 Stephen Picataggio Engineered microorganisms with enhanced fermentation activity
WO2011079388A1 (en) 2009-12-30 2011-07-07 Iogen Energy Corporation Modified yeast strains exhibiting enhanced fermentation of lignocellulosic hydrolysates
CN102190731B (zh) 2010-03-09 2016-01-27 中国科学院上海生命科学研究院 用人工转录因子诱导产生多能干细胞
US9085766B2 (en) 2010-05-21 2015-07-21 Cornell University Methods of producing recombinant heme-binding proteins and uses thereof
WO2012017329A2 (en) 2010-08-02 2012-02-09 Cellectis S.A. Method for targeted genomic events in algae
WO2012083424A1 (en) 2010-12-21 2012-06-28 The University Of Western Ontario Novel amino acid linker sequences for ligand immobilization
EP2669375B1 (en) 2011-01-27 2018-10-17 Kaneka Corporation Yeast of the genus pichia modified to express high levels of a mpp1 homolog and process for producing protein
US9212359B2 (en) 2011-06-23 2015-12-15 Sanford Jay Silverman Systems for induction of gene expression and protein depletion in yeast
CN105724726A (zh) 2011-07-12 2016-07-06 非凡食品有限公司 用于消费品的方法和组合物
US20140220217A1 (en) 2011-07-12 2014-08-07 Maraxi, Inc. Method and compositions for consumables
US10039306B2 (en) 2012-03-16 2018-08-07 Impossible Foods Inc. Methods and compositions for consumables
EP2565262A1 (en) 2011-08-31 2013-03-06 VTU Holding GmbH Protein expression
JP6295512B2 (ja) 2012-03-15 2018-03-20 株式会社豊田中央研究所 酵母における外来遺伝子の発現産物の生産方法、酵母における発現調節剤及びその利用
WO2013158749A2 (en) 2012-04-17 2013-10-24 Gevo, Inc. Engineered microorganisms with improved growth properties
CN104812405A (zh) 2012-09-19 2015-07-29 国立大学法人大阪大学 含有肺炎球菌表面蛋白a的肺炎球菌疫苗
PT2943078T (pt) 2013-01-11 2021-06-16 Impossible Foods Inc Métodos e composições para consumíveis
AU2014305233B2 (en) 2013-08-05 2017-06-29 New Zealand Institute For Bioeconomy Science Limited A method and control system for optimising the quality of meat
SI3044320T1 (sl) 2013-09-11 2020-07-31 Impossible Foods Inc. Sekrecija polipeptidov, ki vsebujejo hem
JP6759103B2 (ja) * 2014-03-31 2020-09-23 インポッシブル フーズ インコーポレイテッド ひき肉レプリカ
WO2016089516A2 (en) 2014-10-31 2016-06-09 Board Of Regents, The University Of Texas System Short exogenous promoter for high level expression in fungi
PT3294762T (pt) 2015-05-11 2022-03-21 Impossible Foods Inc Constructos de expressão e métodos de modificar geneticamente levedura metilotrófica
EP3128006A1 (en) 2015-08-06 2017-02-08 Consejo Superior de Investigaciones Cientificas (CSIC) Tools for multiprotein complex expression in pichia pastoris
US20190292555A1 (en) 2016-12-02 2019-09-26 Impossible Foods Inc. Producing recombinant products in contained systems
US20190292217A1 (en) 2016-12-02 2019-09-26 Impossible Foods Inc. Transgenic plants with upregulated heme biosynthesis
EP3688166A4 (en) 2017-09-28 2021-06-23 Precision Fermentation, Inc. METHODS, DEVICES AND COMPUTER PROGRAM PRODUCTS FOR MONITORING LEEF PERFORMANCE IN FERMENTATION SYSTEMS
RU2658758C1 (ru) 2017-10-02 2018-06-22 ООО "Лаборатория Ангиофарм" Рекомбинантная плазмида для экспрессии в дрожжах Pichia pastoris гена химерного белка ангиогенина человека и штамм дрожжей Pichia pastoris - продуцент рекомбинантного химерного белка ангиогенина человека
US20200325484A1 (en) 2019-04-12 2020-10-15 Delin Liang Enhancing gene expression by linking self-amplifying transcription factor with viral 2A-like peptide
AU2020257270B2 (en) 2019-04-17 2026-01-15 Impossible Foods Inc. Materials and methods for protein production
EP3959311A1 (en) 2019-04-25 2022-03-02 Impossible Foods Inc. Strains and methods for production of heme-containing proteins
US20210062206A1 (en) 2019-07-08 2021-03-04 The Regents Of The University Of California Synthetic transcription factors
KR20240118861A (ko) 2021-12-16 2024-08-05 임파서블 푸즈 인크. 재조합 단백질의 발현을 증가시키는 유전 인자

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014008729A1 (zh) * 2012-07-12 2014-01-16 华东理工大学 消除甲醇诱导型启动子单一甲醇碳源依赖性的方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LIN-CEREGHINO, GP et al., "Mxr1p, A Key Regulator Of The Methanol Utilization Pathway And Peroxisomal Genes In Pichia Pastoris.", Molecular and Cellular Biology., (2006-02), vol. 26, no. 3, pages 883 - 897 *

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