ATE548452T1 - Optische sortiermethode - Google Patents

Optische sortiermethode

Info

Publication number
ATE548452T1
ATE548452T1 AT04077799T AT04077799T ATE548452T1 AT E548452 T1 ATE548452 T1 AT E548452T1 AT 04077799 T AT04077799 T AT 04077799T AT 04077799 T AT04077799 T AT 04077799T AT E548452 T1 ATE548452 T1 AT E548452T1
Authority
AT
Austria
Prior art keywords
nucleic acid
acid molecule
microcapsules
sorting method
concentration
Prior art date
Application number
AT04077799T
Other languages
English (en)
Inventor
Andrew Griffiths
Dan Tawfik
Armin Sepp
Original Assignee
Medical Res Council
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Res Council filed Critical Medical Res Council
Application granted granted Critical
Publication of ATE548452T1 publication Critical patent/ATE548452T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1062—Isolating an individual clone by screening libraries mRNA-Display, e.g. polypeptide and encoding template are connected covalently
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Holo Graphy (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT04077799T 1999-01-07 2000-01-06 Optische sortiermethode ATE548452T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB9900298.2A GB9900298D0 (en) 1999-01-07 1999-01-07 Optical sorting method

Publications (1)

Publication Number Publication Date
ATE548452T1 true ATE548452T1 (de) 2012-03-15

Family

ID=10845700

Family Applications (3)

Application Number Title Priority Date Filing Date
AT00900080T ATE448300T1 (de) 1999-01-07 2000-01-06 Optische sortiermethode
AT04077799T ATE548452T1 (de) 1999-01-07 2000-01-06 Optische sortiermethode
AT04078076T ATE366307T1 (de) 1999-01-07 2000-01-06 Optische sortiermethode

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT00900080T ATE448300T1 (de) 1999-01-07 2000-01-06 Optische sortiermethode

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT04078076T ATE366307T1 (de) 1999-01-07 2000-01-06 Optische sortiermethode

Country Status (11)

Country Link
US (6) US6808882B2 (de)
EP (4) EP1905828B1 (de)
JP (5) JP4851008B2 (de)
AT (3) ATE448300T1 (de)
AU (1) AU781217B2 (de)
CA (3) CA2357037C (de)
DE (2) DE60043289D1 (de)
DK (2) DK1141272T3 (de)
ES (4) ES2333844T3 (de)
GB (1) GB9900298D0 (de)
WO (1) WO2000040712A1 (de)

Families Citing this family (223)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4298912B2 (ja) 1997-07-07 2009-07-22 メディカル リサーチ カウンシル ｉｎｖｉｔｒｏ選別法
US6231521B1 (en) 1998-12-17 2001-05-15 Peter Zoth Audiological screening method and apparatus
GB9900298D0 (en) 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
CA2290731A1 (en) 1999-11-26 2001-05-26 D. Jed Harrison Apparatus and method for trapping bead based reagents within microfluidic analysis system
US6432290B1 (en) 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
GB0022458D0 (en) * 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
FR2816943B1 (fr) * 2000-11-22 2004-02-13 Inst Nat Sante Rech Med Derives de la biotine, leurs procedes de fabrication et leurs utilisations en tant que vecteurs
GB0114854D0 (en) * 2001-06-18 2001-08-08 Medical Res Council Selective gene amplification
GB0115841D0 (en) 2001-06-28 2001-08-22 Medical Res Council Ligand
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
US9321832B2 (en) 2002-06-28 2016-04-26 Domantis Limited Ligand
US20060002935A1 (en) 2002-06-28 2006-01-05 Domantis Limited Tumor Necrosis Factor Receptor 1 antagonists and methods of use therefor
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
JP4664677B2 (ja) 2002-09-06 2011-04-06 イソゲニカ リミテッド イン・ビトロでのペプチド発現ライブラリー
GB0221053D0 (en) * 2002-09-11 2002-10-23 Medical Res Council Single-molecule in vitro evolution
WO2004040314A1 (en) * 2002-10-31 2004-05-13 Chemometec A/S A method for assessment of particles
KR20050088476A (ko) 2002-12-30 2005-09-06 더 리전트 오브 더 유니버시티 오브 캘리포니아 병원균 검출과 분석을 위한 방법과 기구
WO2005003375A2 (en) 2003-01-29 2005-01-13 454 Corporation Methods of amplifying and sequencing nucleic acids
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
US20060078893A1 (en) * 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
GB0307403D0 (en) * 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
EP2127736A1 (de) 2003-04-10 2009-12-02 The President and Fellows of Harvard College Erzeugung und Kontrolle von Fluiden
US20040265835A1 (en) * 2003-06-30 2004-12-30 Lemaster David M. Method of sorting vesicle-entrapped, coupled nucleic acid-protein displays
EP1641809B2 (de) 2003-07-05 2018-10-03 The Johns Hopkins University Verfahren und zusammensetzungen zum nachweis und zur auflistung genetischer variationen
CN104069784B (zh) 2003-08-27 2017-01-11 哈佛大学 流体物种的电子控制
GB0325653D0 (en) 2003-11-03 2003-12-10 Medical Res Council CST emulsions
WO2005049787A2 (en) * 2003-11-24 2005-06-02 Yeda Research And Development Co.Ltd. Compositions and methods for in vitro sorting of molecular and cellular libraries
CA2553833C (en) * 2004-01-28 2012-10-02 454 Corporation Nucleic acid amplification with continuous flow emulsion
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US7799553B2 (en) * 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
US7081227B2 (en) * 2004-06-07 2006-07-25 The Reagents Of The University Of California Amphiphilic mediated sample preparation for micro-flow cytometry
US9477233B2 (en) 2004-07-02 2016-10-25 The University Of Chicago Microfluidic system with a plurality of sequential T-junctions for performing reactions in microdroplets
US7655470B2 (en) 2004-10-29 2010-02-02 University Of Chicago Method for manipulating a plurality of plugs and performing reactions therein in microfluidic systems
EP2261650A3 (de) 2004-09-15 2011-07-06 IntegenX Inc. Mikrofluidische Vorrichtungen
KR20070084069A (ko) 2004-10-08 2007-08-24 도만티스 리미티드 Ｔｎｆｒ1에 대한 단일 도메인 항체 및 이의 사용 방법
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
WO2006084132A2 (en) 2005-02-01 2006-08-10 Agencourt Bioscience Corp. Reagents, methods, and libraries for bead-based squencing
EP2248578B1 (de) 2005-03-04 2012-06-06 President and Fellows of Harvard College Verfahren zur Bildung mehrerer Emulsionen
US20070054119A1 (en) * 2005-03-04 2007-03-08 Piotr Garstecki Systems and methods of forming particles
US20060269934A1 (en) * 2005-03-16 2006-11-30 Applera Corporation Compositions and methods for clonal amplification and analysis of polynucleotides
EP2428579B1 (de) * 2005-10-24 2013-05-29 The Johns Hopkins University Verbessertes Verfahren zum BEAMing
JP2009536313A (ja) 2006-01-11 2009-10-08 レインダンス テクノロジーズ， インコーポレイテッド ナノリアクターの形成および制御において使用するマイクロ流体デバイスおよび方法
WO2007089541A2 (en) * 2006-01-27 2007-08-09 President And Fellows Of Harvard College Fluidic droplet coalescence
US7749365B2 (en) 2006-02-01 2010-07-06 IntegenX, Inc. Optimized sample injection structures in microfluidic separations
CN101400703B (zh) 2006-02-01 2013-05-08 赛法隆澳大利亚控股有限公司 结构域抗体构建体
JP5063616B2 (ja) 2006-02-03 2012-10-31 インテジェニックス インコーポレイテッド マイクロ流体デバイス
CN101371124B (zh) * 2006-02-13 2012-02-29 新加坡科技研究局 处理生物样品和/或化学样品的方法
US7766033B2 (en) 2006-03-22 2010-08-03 The Regents Of The University Of California Multiplexed latching valves for microfluidic devices and processors
ATE540750T1 (de) 2006-05-11 2012-01-15 Raindance Technologies Inc Mikrofluidische vorrichtung und verfahren
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US20080076909A1 (en) * 2006-06-30 2008-03-27 Applera Corporation Emulsion pcr and amplicon capture
EP3536396B1 (de) 2006-08-07 2022-03-30 The President and Fellows of Harvard College Fluorkohlenstoffemulsionsstabilisierende tenside
WO2008052138A2 (en) 2006-10-25 2008-05-02 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated dna analysis system using same
KR20100028526A (ko) 2007-02-05 2010-03-12 마이크로칩 바이오테크놀로지스, 인크. 마이크로유체 및 나노유체 장치, 시스템 및 응용
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US7776927B2 (en) * 2007-03-28 2010-08-17 President And Fellows Of Harvard College Emulsions and techniques for formation
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US8691164B2 (en) * 2007-04-20 2014-04-08 Celula, Inc. Cell sorting system and methods
US7883265B2 (en) * 2007-06-01 2011-02-08 Applied Biosystems, Llc Devices, systems, and methods for preparing emulsions
WO2009015296A1 (en) 2007-07-24 2009-01-29 The Regents Of The University Of California Microfabricated dropley generator
WO2009021215A1 (en) * 2007-08-09 2009-02-12 Celula, Inc. Methods and devices for correlated, multi-parameter single cell measurements and recovery of remnant biological material
JP5431351B2 (ja) 2007-11-27 2014-03-05 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド バイサルファイト修飾された核酸を増幅およびコピーするための酵素
AU2008328726B2 (en) 2007-11-30 2014-06-12 Glaxo Group Limited Antigen-binding constructs
JP5738597B2 (ja) 2007-12-21 2015-06-24 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸の配列決定のためのシステムおよび方法
KR20110030415A (ko) 2008-01-22 2011-03-23 인터젠엑스 인크. 만능 샘플 제조 시스템 및 집적 분석 시스템에서의 용도
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
EP2315629B1 (de) 2008-07-18 2021-12-15 Bio-Rad Laboratories, Inc. Tröpfchenbibliotheken
US20110218123A1 (en) 2008-09-19 2011-09-08 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
WO2011120006A1 (en) 2010-03-25 2011-09-29 Auantalife, Inc. A Delaware Corporation Detection system for droplet-based assays
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US12090480B2 (en) 2008-09-23 2024-09-17 Bio-Rad Laboratories, Inc. Partition-based method of analysis
CA2738578C (en) 2008-09-23 2021-05-04 Quantalife, Inc. Droplet-based assay system
US8633015B2 (en) 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US9399215B2 (en) 2012-04-13 2016-07-26 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US12162008B2 (en) 2008-09-23 2024-12-10 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
WO2011120024A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet generation for droplet-based assays
GB0822261D0 (en) 2008-12-05 2009-01-14 Merten Christoph Assay
GB0822260D0 (en) 2008-12-05 2009-01-14 Merten Christoph Assay
WO2010080134A1 (en) 2008-12-19 2010-07-15 President And Fellows Of Harvard College Particle-assisted nucleic acid sequencing
CN102341691A (zh) 2008-12-31 2012-02-01 尹特根埃克斯有限公司 具有微流体芯片的仪器
JP2012518398A (ja) 2009-02-24 2012-08-16 グラクソ グループ リミテッド 抗原結合性構築物
US20110305694A1 (en) 2009-02-24 2011-12-15 Paul Andrew Hamblin Multivalent and/or multispecific rankl-binding constructs
WO2010097386A1 (en) 2009-02-24 2010-09-02 Glaxo Group Limited Antigen-binding constructs
WO2010104597A2 (en) 2009-03-13 2010-09-16 President And Fellows Of Harvard College Scale-up of microfluidic devices
GB0904957D0 (en) 2009-03-23 2009-05-06 Univ Erasmus Medical Ct Tumour gene profile
EP3415235B1 (de) 2009-03-23 2025-11-12 Bio-Rad Laboratories, Inc. Manipulation von mikrofluidiktröpfchen
WO2010117457A2 (en) * 2009-04-08 2010-10-14 Applied Biosystems, Llc System comprising dual-sided thermal cycler and emulsion pcr in pouch
US8388908B2 (en) 2009-06-02 2013-03-05 Integenx Inc. Fluidic devices with diaphragm valves
JP2012529268A (ja) 2009-06-05 2012-11-22 インテジェンクス，インコーポレイテッド ユニバーサルサンプル調製システムおよび統合解析システムの使用方法
JP5099455B2 (ja) * 2009-07-16 2012-12-19 独立行政法人科学技術振興機構 エマルジョンを用いたｒｎａの選択的増幅法
US20120211084A1 (en) 2009-09-02 2012-08-23 President And Fellows Of Harvard College Multiple emulsions created using jetting and other techniques
EP2473618B1 (de) 2009-09-02 2015-03-04 Bio-Rad Laboratories, Inc. System zum mischen von flüssigkeiten durch gerinnung mehrerer emulsionen
CN102549445B (zh) 2009-09-28 2015-04-08 皇家飞利浦电子股份有限公司 用于单个颗粒检测的生物传感器系统
WO2011042564A1 (en) 2009-10-09 2011-04-14 Universite De Strasbourg Labelled silica-based nanomaterial with enhanced properties and uses thereof
GB0918564D0 (en) 2009-10-22 2009-12-09 Plasticell Ltd Nested cell encapsulation
CN102648053B (zh) 2009-10-27 2016-04-27 哈佛学院院长等 液滴生成技术
US10207240B2 (en) * 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US8584703B2 (en) 2009-12-01 2013-11-19 Integenx Inc. Device with diaphragm valve
EP2517025B1 (de) 2009-12-23 2019-11-27 Bio-Rad Laboratories, Inc. Verfahren zur reduzierung des austauschs von molekülen zwischen tröpfchen
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
CA2789425C (en) 2010-02-12 2020-04-28 Raindance Technologies, Inc. Digital analyte analysis with polymerase error correction
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
JP6155419B2 (ja) 2010-03-25 2017-07-05 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド 検出用の液滴輸送システム
US8512538B2 (en) 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
US9121058B2 (en) 2010-08-20 2015-09-01 Integenx Inc. Linear valve arrays
WO2012024657A1 (en) 2010-08-20 2012-02-23 IntegenX, Inc. Microfluidic devices with mechanically-sealed diaphragm valves
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
CN103429331B (zh) 2010-11-01 2016-09-28 伯乐生命医学产品有限公司 用于形成乳液的系统
US20130236467A1 (en) 2010-11-24 2013-09-12 Jeremy Griggs Multispecific antigen binding proteins targeting hgf
EP3859011A1 (de) 2011-02-11 2021-08-04 Bio-Rad Laboratories, Inc. Verfahren zur bildung gemischter tröpfchen
EP3736281A1 (de) 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Zusammensetzungen und verfahren für molekulare etikettierung
US12097495B2 (en) 2011-02-18 2024-09-24 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
AU2012231098B2 (en) 2011-03-18 2016-09-29 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
US9347059B2 (en) 2011-04-25 2016-05-24 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
GB201108259D0 (en) * 2011-05-17 2011-06-29 Cambridge Entpr Ltd Gel beads in microfluidic droplets
EP2714254B1 (de) 2011-05-23 2017-09-06 President and Fellows of Harvard College Kontrolle von emulsionen, einschliesslich mehrerer emulsionen
EP3709018A1 (de) 2011-06-02 2020-09-16 Bio-Rad Laboratories, Inc. Mikrofluidische vorrichtung zum nachweis von komponenten einer chemischen reaktion
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
WO2012170907A2 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
FI3461807T3 (fi) 2011-06-08 2023-09-07 Life Technologies Corp Uudenlaisten detergenttien suunnittelu ja kehitys pcr-järjestelmissä käyttöä varten
BR112014000141A2 (pt) 2011-07-06 2017-06-13 Harvard College emulsões múltiplas e técnicas para a formação de emulsões múltiplas
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
EP2737089B1 (de) 2011-07-29 2017-09-06 Bio-rad Laboratories, Inc. Bibliothekscharakterisierung durch digitalen test
US20150136604A1 (en) 2011-10-21 2015-05-21 Integenx Inc. Sample preparation, processing and analysis systems
US10865440B2 (en) 2011-10-21 2020-12-15 IntegenX, Inc. Sample preparation, processing and analysis systems
GB201120711D0 (en) 2011-12-01 2012-01-11 Univ Erasmus Medical Ct Method for classifying tumour cells
US9701959B2 (en) 2012-02-02 2017-07-11 Invenra Inc. High throughput screen for biologically active polypeptides
CA3216609C (en) 2012-08-14 2024-05-14 10X Genomics, Inc. Microcapsule compositions and methods
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
AU2014214682B2 (en) 2013-02-08 2018-07-26 10X Genomics, Inc. Polynucleotide barcode generation
TWI707038B (zh) 2013-08-05 2020-10-11 美商扭轉生物科技有限公司 重新合成之基因庫
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
CN103487420B (zh) * 2013-09-29 2015-08-12 浙江大学 一种制备微胶囊pH传感器的方法
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
EP3539944A1 (de) 2013-10-25 2019-09-18 Life Technologies Corporation Neuartige verbindungen zur verwendung in pcr-systemen und anwendungen davon
EP3065712A4 (de) 2013-11-08 2017-06-21 President and Fellows of Harvard College Mikropartikel, verfahren zu deren herstellung und verwendung
CN105873681B (zh) 2013-11-18 2019-10-11 尹特根埃克斯有限公司 用于样本分析的卡盒和仪器
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
US11193176B2 (en) 2013-12-31 2021-12-07 Bio-Rad Laboratories, Inc. Method for detecting and quantifying latent retroviral RNA species
WO2015157567A1 (en) 2014-04-10 2015-10-15 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
WO2015179098A1 (en) 2014-05-21 2015-11-26 Integenx Inc. Fluidic cartridge with valve mechanism
CN113249435B (zh) 2014-06-26 2024-09-03 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
MX2016016904A (es) 2014-06-26 2017-03-27 10X Genomics Inc Analisis de secuencias de acidos nucleicos.
JP2017526046A (ja) 2014-06-26 2017-09-07 １０エックス ゲノミクス，インコーポレイテッド 核酸配列アセンブルのプロセス及びシステム
KR101776368B1 (ko) * 2014-10-02 2017-09-07 서울시립대학교 산학협력단 전령 ｒｎａ 나노입자 및 그 제조방법
US10690627B2 (en) 2014-10-22 2020-06-23 IntegenX, Inc. Systems and methods for sample preparation, processing and analysis
MX2017005267A (es) 2014-10-29 2017-07-26 10X Genomics Inc Metodos y composiciones para la secuenciacion de acidos nucleicos seleccionados como diana.
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
EP3245605B1 (de) 2015-01-13 2022-04-20 10X Genomics, Inc. Systeme und verfahren zur visualisierung von informationen zu struktureller variation und phase
CA2975855C (en) 2015-02-04 2025-09-23 Twist Bioscience Corporation SYNTHETIC GENE COMPOSITIONS AND ASSEMBLY METHODS
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016130578A1 (en) 2015-02-09 2016-08-18 10X Genomics, Inc. Systems and methods for determining structural variation and phasing using variant call data
EP4286516A3 (de) 2015-02-24 2024-03-06 10X Genomics, Inc. Partitionsverarbeitungsverfahren und -systeme
BR112017018054A2 (pt) 2015-02-24 2018-07-24 10X Genomics Inc métodos para a cobertura de sequências de ácidos nucleicos direcionadas
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
KR20180050411A (ko) 2015-09-18 2018-05-14 트위스트 바이오사이언스 코포레이션 올리고핵산 변이체 라이브러리 및 그의 합성
KR102794025B1 (ko) 2015-09-22 2025-04-09 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
CA3001679A1 (en) 2015-10-13 2017-04-20 President And Fellows Of Harvard College Systems and methods for making and using gel microspheres
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
JP6735348B2 (ja) 2016-02-11 2020-08-05 １０エックス ジェノミクス， インコーポレイテッド 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
JP6854340B2 (ja) 2016-08-22 2021-04-07 ツイスト バイオサイエンス コーポレーション デノボ合成された核酸ライブラリ
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
KR102514213B1 (ko) 2016-12-16 2023-03-27 트위스트 바이오사이언스 코포레이션 면역 시냅스의 변이체 라이브러리 및 그의 합성
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN110214186B (zh) 2017-01-30 2023-11-24 10X基因组学有限公司 用于基于微滴的单细胞条形编码的方法和系统
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
CN110892485B (zh) 2017-02-22 2024-03-22 特韦斯特生物科学公司 基于核酸的数据存储
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
GB201707143D0 (en) 2017-05-04 2017-06-21 Plasticell Ltd Method for producing cells
EP3625715A4 (de) 2017-05-19 2021-03-17 10X Genomics, Inc. Systeme und verfahren zur analyse von datensätzen
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN109526228B (zh) 2017-05-26 2022-11-25 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
US10696965B2 (en) 2017-06-12 2020-06-30 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
EP3954782A1 (de) 2017-11-15 2022-02-16 10X Genomics, Inc. Funktionalisierte gelperlen
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
KR102804057B1 (ko) 2018-01-04 2025-05-07 트위스트 바이오사이언스 코포레이션 Dna 기반 디지털 정보 저장
US10961518B2 (en) 2018-01-19 2021-03-30 Bio-Rad Laboratories, Inc. Mutant DNA polymerases
CN112262218B (zh) 2018-04-06 2024-11-08 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
CA3100739A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
US12357959B2 (en) 2018-12-26 2025-07-15 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
CA3131691A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
CN113766930B (zh) 2019-02-26 2025-07-22 特韦斯特生物科学公司 Glp1受体的变异核酸文库
JP2020143015A (ja) * 2019-03-06 2020-09-10 ソニー株式会社 粒子標識用試薬、粒子染色方法、及び顕微鏡システム
AU2020298294A1 (en) 2019-06-21 2022-02-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
WO2021061842A1 (en) 2019-09-23 2021-04-01 Twist Bioscience Corporation Variant nucleic acid libraries for single domain antibodies
JP2022548309A (ja) 2019-09-23 2022-11-17 ツイスト バイオサイエンス コーポレーション Ｃｒｔｈ２のバリアント核酸ライブラリー
IL293670A (en) 2019-12-09 2022-08-01 Twist Bioscience Corp Variable nucleic acid libraries for adenosine receptors
CN115428088A (zh) 2020-02-13 2022-12-02 10X基因组学有限公司 用于基因表达和dna染色质可及性的联合交互式可视化的系统和方法

Family Cites Families (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4533634A (en) * 1983-01-26 1985-08-06 Amf Inc. Tissue culture medium
US4675285A (en) * 1984-09-19 1987-06-23 Genetics Institute, Inc. Method for identification and isolation of DNA encoding a desired protein
US4801529A (en) * 1985-06-18 1989-01-31 Brandeis University Methods for isolating mutant microoganisms using microcapsules coated with indicator material
GB9107628D0 (en) * 1991-04-10 1991-05-29 Moonbrook Limited Preparation of diagnostic agents
US5177961A (en) * 1991-06-26 1993-01-12 W. R. Grace & Co.-Conn. Upstream collimator for electrically heatable catalytic converter
WO1993003151A1 (en) * 1991-08-10 1993-02-18 Medical Research Council Treatment of cell populations
DE69223980T2 (de) * 1991-10-15 1998-05-28 Multilyte Ltd Bindungstest unter benutzung eines markierten reagens
US5270170A (en) * 1991-10-16 1993-12-14 Affymax Technologies N.V. Peptide library and screening method
US5413924A (en) * 1992-02-13 1995-05-09 Kosak; Kenneth M. Preparation of wax beads containing a reagent for release by heating
US5489508A (en) * 1992-05-13 1996-02-06 University Of Texas System Board Of Regents Therapy and diagnosis of conditions related to telomere length and/or telomerase activity
DE4223169C1 (de) * 1992-07-10 1993-11-25 Ferring Arzneimittel Gmbh Verfahren zur Mikroverkapselung wasserlöslicher Wirkstoffe
US5525504A (en) * 1993-04-30 1996-06-11 Merck Patent Gesellschaft Mit Beschrankter Haftung Cytolysin gene and gene product
JPH09501100A (ja) * 1993-12-30 1997-02-04 エフ エム シー コーポレーション 高い内部相含量をもつ水／油型エマルジョン及び水／油／水型エマルジョン
EP0804249A2 (de) * 1994-03-15 1997-11-05 Brown University Research Foundation Polymeres system zur freisetzung von genen
KR100501838B1 (ko) * 1995-04-24 2005-07-21 큐비스트 파마슈티컬즈 인코포레이티드 새로운 대사 경로를 만들고 이를 스크리닝하는 방법
US5989892A (en) * 1995-06-14 1999-11-23 Tonen Corporation Microorganisms, demulsifiers and processes for breaking an emulsion
AU725144B2 (en) * 1995-09-22 2000-10-05 Terragen Diversity Inc. Method for isolating xylanase gene sequences from soil DNA, compositions useful in such method and compositions obtained thereby
DE19646372C1 (de) * 1995-11-11 1997-06-19 Evotec Biosystems Gmbh Genotyp und Phänotyp koppelnde Verbindung
DE19543366C2 (de) * 1995-11-21 1998-09-10 Stockhausen Chem Fab Gmbh Mit ungesättigten Aminoalkoholen vernetzte, wasserquellbare Polymerisate, deren Herstellung und Verwendung
US6011137A (en) * 1996-04-03 2000-01-04 University Of North Carolina At Chapel Hill Identification and isolation of novel polypeptides having WW domains and methods of using same
GB9608540D0 (en) * 1996-04-25 1996-07-03 Medical Res Council Isolation of enzymes
US5876711A (en) * 1996-05-31 1999-03-02 Onyx Pharmaceuticals, Inc. Methods and compositions for determining the tumor suppressor status of cells
IL120466A0 (en) * 1997-03-17 1997-07-13 Intelligene Ltd In vitro evolution of oligonucleotides
US5902727A (en) * 1996-09-04 1999-05-11 Washington University Method for localization and quantitation of a substance in a biological sample
EP0932686A2 (de) * 1996-10-01 1999-08-04 Geron Corporation Telomerase reverse-transkriptase
US6310354B1 (en) * 1996-12-03 2001-10-30 Erkki Soini Method and a device for monitoring nucleic acid amplification reactions
DK1712623T3 (da) * 1997-01-21 2012-02-06 Gen Hospital Corp Udvælgelse af proteiner ved anvendelse af RNA-proteinfusioner
US6023540A (en) * 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
CA2284066A1 (en) * 1997-03-18 1998-09-24 Chromaxome Corporation Methods for screening compounds using encapsulated cells
US5958738A (en) * 1997-03-24 1999-09-28 Roche Diagnostics Corporation Procedure for subtractive hybridization and difference analysis
US6048551A (en) * 1997-03-27 2000-04-11 Hilfinger; John M. Microsphere encapsulation of gene transfer vectors
AU8162598A (en) * 1997-06-25 1999-01-04 Life Technologies, Inc. Improved method for isolating and recovering target dna or rna molecules having a desired nucleotide sequence
JP4298912B2 (ja) * 1997-07-07 2009-07-22 メディカル リサーチ カウンシル ｉｎｖｉｔｒｏ選別法
US6348278B1 (en) * 1998-06-09 2002-02-19 Mobil Oil Corporation Method and system for supplying hydrogen for use in fuel cells
JP2981547B1 (ja) * 1998-07-02 1999-11-22 農林水産省食品総合研究所長 クロスフロー型マイクロチャネル装置及び同装置を用いたエマルションの生成または分離方法
GB9900298D0 (en) * 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
CN1189159C (zh) * 2000-05-05 2005-02-16 欧莱雅 含水溶性美容活性组分水性核的微胶囊及含其的组合物
GB0022458D0 (en) * 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
US6936264B2 (en) * 2001-03-05 2005-08-30 The Procter & Gamble Company Delivery of reactive agents via multiple emulsions for use in shelf stable products
GB0127564D0 (en) * 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
WO2005003375A2 (en) * 2003-01-29 2005-01-13 454 Corporation Methods of amplifying and sequencing nucleic acids

Also Published As

Publication number Publication date
AU1884400A (en) 2000-07-24
US20090053700A1 (en) 2009-02-26
EP1522582A8 (de) 2005-06-08
DE60035442T2 (de) 2008-03-20
EP1905828A3 (de) 2009-05-20
JP2011206056A (ja) 2011-10-20
EP1522582A3 (de) 2005-05-11
EP1141272A1 (de) 2001-10-10
EP1522582A2 (de) 2005-04-13
ES2333844T3 (es) 2010-03-02
EP1522581B1 (de) 2012-03-07
ES2293161T3 (es) 2008-03-16
AU781217B2 (en) 2005-05-12
US20020119459A1 (en) 2002-08-29
US20090325236A1 (en) 2009-12-31
CA2584933A1 (en) 2000-07-13
ATE448300T1 (de) 2009-11-15
US20050164239A1 (en) 2005-07-28
CA2357037C (en) 2012-12-11
JP2005245446A (ja) 2005-09-15
DE60035442D1 (de) 2007-08-16
JP4851008B2 (ja) 2012-01-11
JP2007143564A (ja) 2007-06-14
US6808882B2 (en) 2004-10-26
ES2392990T3 (es) 2012-12-17
EP1522581A3 (de) 2005-05-04
JP2011092218A (ja) 2011-05-12
DE60043289D1 (de) 2009-12-24
EP1522582B1 (de) 2007-07-04
EP1905828B1 (de) 2012-08-08
EP1141272B1 (de) 2009-11-11
US7897341B2 (en) 2011-03-01
US20140187762A1 (en) 2014-07-03
WO2000040712A1 (en) 2000-07-13
EP1522581A2 (de) 2005-04-13
US20050037392A1 (en) 2005-02-17
JP2002534085A (ja) 2002-10-15
GB9900298D0 (en) 1999-02-24
JP5198597B2 (ja) 2013-05-15
EP1905828A2 (de) 2008-04-02
CA2357037A1 (en) 2000-07-13
ATE366307T1 (de) 2007-07-15
DK1522581T3 (da) 2012-04-10
ES2386567T3 (es) 2012-08-23
DK1141272T3 (da) 2010-03-29
CA2479551A1 (en) 2000-07-13

Similar Documents

Publication Publication Date Title
ATE548452T1 (de) Optische sortiermethode
ATE487790T1 (de) In-vitro-sortierverfahren
DK1446504T3 (da) Emulsionssammensætninger
NO950381L (no) Nukleinsyresekvens amplifikasjon
ATE386044T1 (de) Isolierung von nukleinsäuren
EP1964932A3 (de) Verfahren zur zielgerichteten Evolution
DE69326685D1 (de) Amplifikation von test reporters durch nukleinsäure replikation
DE69305662D1 (de) Brett-stabiles Produkt und Verfahren zur Isolierung der RNA, DNA und Proteine
ATE485372T1 (de) Nukleinsäurenachweisverfahren und system dafür
ATE348193T1 (de) Verfahren und vorrichtung zur bestimmung von amplifizierten genprodukten zum nachweis eines gens
DE50306215D1 (de) Verfahren zum einkapseln gel ster farbreaktionspartner von f arbreaktionssystemen, die danach erh ltlichen kapseln sowie deren verwendung in farbreaktionspapieren