AR034121A1 - Metodo para aislar celulas de esperma que contienen cromosoma x de celulas de esperma que contienen cromosoma y - Google Patents

Metodo para aislar celulas de esperma que contienen cromosoma x de celulas de esperma que contienen cromosoma y

Info

Publication number
AR034121A1
AR034121A1 ARP010102198A ARP010102198A AR034121A1 AR 034121 A1 AR034121 A1 AR 034121A1 AR P010102198 A ARP010102198 A AR P010102198A AR P010102198 A ARP010102198 A AR P010102198A AR 034121 A1 AR034121 A1 AR 034121A1
Authority
AR
Argentina
Prior art keywords
sperm cells
chromosome
drops
cells containing
transported
Prior art date
Application number
ARP010102198A
Other languages
English (en)
Inventor
Kenneth M Evans
Munster Eric B Van
Original Assignee
Xy Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27394507&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AR034121(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Xy Inc filed Critical Xy Inc
Publication of AR034121A1 publication Critical patent/AR034121A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6879—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for sex determination
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03B—SEPARATING SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS
    • B03B9/00—General arrangement of separating plant, e.g. flow sheets
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/061—Sperm cells, spermatogonia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
    • G01F17/00—Methods or apparatus for determining the capacity of containers or cavities, or the volume of solid bodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1468—Electro-optical investigation, e.g. flow cytometers with spatial resolution of the texture or inner structure of the particle
    • G01N15/147—Electro-optical investigation, e.g. flow cytometers with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/149—

Abstract

Un método para aislar células de esperma que contiene cromosoma X y células de esperma que contiene cromosoma Y, caracterizado porque comprende los pasos de: a) recolectar las células de esperma de un macho de una especie de mamífero; b) determinar las características de diferenciación de dichas células de esperma; c) diferenciar entre dichas células de esperma basadas sobre dicha característica de diferenciación de sexo; d) separar las células de esperma diferenciadas en una población que contiene cromosoma X y una población que contiene cromosoma Y; y e) producir separadamente las poblaciones que contienen el cromosoma X y el cromosoma Y de las células de esperma, cada una presentando una puridad mayor que 90%. Un método para aislar células de esperma que contienen cromosoma X y células de esperma que contienen cromosoma Y, caracterizado porque comprende los pasos de: a) introducir dichas células de esperma dentro de una corriente de fluido; b) formar gotas de una pluralidad teniendo una de dichas células de esperma transportada; c) analizar dichas células de esperma transportadas en dicha corriente de flujo; d) cargar cada una de dichas gotas diferencialmente basado sobre dicha característica de diferenciación de sexo de dichas células de esperma transportadas en dichas gotas; e) desviar cada una de dichas gotas; f) diferencialmente recolectar cada una de dichas gotas basadas sobre dichas características de diferenciación de sexo de dichas células de esperma transportadas en dichas gotas.
ARP010102198A 2000-05-09 2001-05-09 Metodo para aislar celulas de esperma que contienen cromosoma x de celulas de esperma que contienen cromosoma y AR034121A1 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US20308900P 2000-05-09 2000-05-09
US23975200P 2000-10-12 2000-10-12
US26757101P 2001-02-10 2001-02-10

Publications (1)

Publication Number Publication Date
AR034121A1 true AR034121A1 (es) 2004-02-04

Family

ID=27394507

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP010102198A AR034121A1 (es) 2000-05-09 2001-05-09 Metodo para aislar celulas de esperma que contienen cromosoma x de celulas de esperma que contienen cromosoma y

Country Status (21)

Country Link
US (7) US7371517B2 (es)
EP (8) EP2258170B1 (es)
JP (4) JP5071995B2 (es)
KR (1) KR100849948B1 (es)
CN (4) CN104004710B (es)
AR (1) AR034121A1 (es)
AU (4) AU2001263039B2 (es)
BR (1) BR0110731A (es)
CA (1) CA2408939C (es)
DK (3) DK2258170T3 (es)
HK (2) HK1163810A1 (es)
HU (1) HUP0302232A2 (es)
IL (2) IL152714A (es)
MX (1) MXPA02010971A (es)
NO (1) NO20025370L (es)
NZ (4) NZ522613A (es)
PL (1) PL359598A1 (es)
RU (2) RU2393815C2 (es)
UA (2) UA95899C2 (es)
WO (1) WO2001085913A2 (es)
ZA (1) ZA200209139B (es)

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