WO2003056330A3 - Zellsortiersystem zur grössenabhängigen sortierung oder separation von in einer strömenden flüssigkeit suspendierten zellen - Google Patents

Zellsortiersystem zur grössenabhängigen sortierung oder separation von in einer strömenden flüssigkeit suspendierten zellen Download PDF

Info

Publication number
WO2003056330A3
WO2003056330A3 PCT/EP2002/014764 EP0214764W WO03056330A3 WO 2003056330 A3 WO2003056330 A3 WO 2003056330A3 EP 0214764 W EP0214764 W EP 0214764W WO 03056330 A3 WO03056330 A3 WO 03056330A3
Authority
WO
WIPO (PCT)
Prior art keywords
size
separation
flowing fluid
cells suspended
cell sorting
Prior art date
Application number
PCT/EP2002/014764
Other languages
English (en)
French (fr)
Other versions
WO2003056330A2 (de
Inventor
Dirk Lassner
Holm Uhlig
Johann Michael Koehler
Guenter Mayer
Original Assignee
Inst Physikalische Hochtech Ev
Dirk Lassner
Holm Uhlig
Johann Michael Koehler
Guenter Mayer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Physikalische Hochtech Ev, Dirk Lassner, Holm Uhlig, Johann Michael Koehler, Guenter Mayer filed Critical Inst Physikalische Hochtech Ev
Priority to AU2002363895A priority Critical patent/AU2002363895A1/en
Publication of WO2003056330A2 publication Critical patent/WO2003056330A2/de
Publication of WO2003056330A3 publication Critical patent/WO2003056330A3/de

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484Optical investigation techniques, e.g. flow cytometry microstructural devices
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/149Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Ecology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Separation Of Solids By Using Liquids Or Pneumatic Power (AREA)

Abstract

Die Erfindung betrifft Zellsortiersystem zur größenabhängigen Sortierung oder Separation von in einer strömenden Flüssigkeit suspendierten Zellen. Die Zellsortiersystem zeichnet sich dadurch aus, dass es zumindest einen Chip (10, 40, 42), der eine von der Flüssigkeit überströmte Grundplatte (12) mit einer Vielzahl von Vertiefungen (31, 60, 62, 70, 80) beinhaltet, umfasst.
PCT/EP2002/014764 2001-12-31 2002-12-27 Zellsortiersystem zur grössenabhängigen sortierung oder separation von in einer strömenden flüssigkeit suspendierten zellen WO2003056330A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002363895A AU2002363895A1 (en) 2001-12-31 2002-12-27 Cell sorting system for the size-based sorting or separation of cells suspended in a flowing fluid

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10164578.3 2001-12-31
DE10164578 2001-12-31

Publications (2)

Publication Number Publication Date
WO2003056330A2 WO2003056330A2 (de) 2003-07-10
WO2003056330A3 true WO2003056330A3 (de) 2003-10-09

Family

ID=7711209

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/014764 WO2003056330A2 (de) 2001-12-31 2002-12-27 Zellsortiersystem zur grössenabhängigen sortierung oder separation von in einer strömenden flüssigkeit suspendierten zellen

Country Status (2)

Country Link
AU (1) AU2002363895A1 (de)
WO (1) WO2003056330A2 (de)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8187894B2 (en) 2003-09-30 2012-05-29 Alcatel Lucent Method and apparatus for controlling the flow resistance of a fluid on nanostructured or microstructured surfaces
US9145540B1 (en) 2007-11-15 2015-09-29 Seng Enterprises Ltd. Device for the study of living cells
US9200245B2 (en) 2003-06-26 2015-12-01 Seng Enterprises Ltd. Multiwell plate

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998034094A1 (en) 1997-01-31 1998-08-06 The Horticulture & Food Research Institute Of New Zealand Ltd. Optical apparatus
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
AU2002237689B2 (en) 2000-11-29 2008-01-10 Xy, Llc. System to separate frozen-thawed spermatozoa into X-chromosome bearing and Y-chromosome bearing populations
EP2275533B9 (de) 2002-08-01 2016-10-19 Xy, Llc Verfahren zur Schätzung von Spermzellen
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
WO2004017041A2 (en) 2002-08-15 2004-02-26 Xy, Inc. High resolution flow cytometer
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
IL154677A0 (en) 2003-02-27 2003-09-17 Univ Bar Ilan A method and apparatus for manipulating an individual cell
ES2561816T3 (es) 2003-03-28 2016-03-01 Inguran, Llc Aparatos, métodos y procesos para clasificar partículas y para proporcionar esperma animal clasificado por sexo
CA2566749C (en) 2003-05-15 2017-02-21 Xy, Inc. Efficient haploid cell sorting for flow cytometer systems
US7888110B2 (en) 2003-06-26 2011-02-15 Seng Enterprises Ltd. Pico liter well holding device and method of making the same
US7544805B2 (en) 2004-02-03 2009-06-09 Chemagis Ltd. Stable amorphous forms of montelukast sodium
MXPA06011344A (es) 2004-03-29 2006-12-15 Monsanto Technology Llc Suspensiones de espermatozoides para separar en poblaciones enriquecidas con cromosomas x o y.
US7403647B2 (en) 2004-09-13 2008-07-22 Seng Enterprises Ltd. Method for identifying an image of a well in an image of a well-bearing component
BRPI0513685A (pt) 2004-07-22 2008-05-13 Monsanto Technology Llc processo para enriquecimento de uma população de células de esperma
DE602005023534D1 (de) * 2005-01-25 2010-10-21 Seng Entpr Ltd Mikrofluidische vorrichtung zur untersuchung zellen
US8287808B2 (en) 2005-09-15 2012-10-16 Alcatel Lucent Surface for reversible wetting-dewetting
US8721161B2 (en) 2005-09-15 2014-05-13 Alcatel Lucent Fluid oscillations on structured surfaces
US7412938B2 (en) 2005-09-15 2008-08-19 Lucent Technologies Inc. Structured surfaces with controlled flow resistance
US8734003B2 (en) 2005-09-15 2014-05-27 Alcatel Lucent Micro-chemical mixing
US9975118B2 (en) 2007-11-15 2018-05-22 Seng Enterprises Ltd. Device for the study of living cells
IT1391408B1 (it) 2008-10-02 2011-12-23 Silicon Biosystems Spa Camera di separazione

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5310674A (en) * 1982-05-10 1994-05-10 Bar-Ilan University Apertured cell carrier
WO1995001559A2 (de) * 1993-07-02 1995-01-12 Evotec Biosystems Gmbh Probenträger und seine verwendung
DE19705910C1 (de) * 1997-02-15 1998-06-18 Inst Physikalische Hochtech Ev Mikrokammerarray mit hoher Kammerdichte
WO2001094635A2 (en) * 2000-06-05 2001-12-13 California Institute Of Technology Integrated active flux microfluidic devices and methods

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5310674A (en) * 1982-05-10 1994-05-10 Bar-Ilan University Apertured cell carrier
WO1995001559A2 (de) * 1993-07-02 1995-01-12 Evotec Biosystems Gmbh Probenträger und seine verwendung
DE19705910C1 (de) * 1997-02-15 1998-06-18 Inst Physikalische Hochtech Ev Mikrokammerarray mit hoher Kammerdichte
WO2001094635A2 (en) * 2000-06-05 2001-12-13 California Institute Of Technology Integrated active flux microfluidic devices and methods

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MAYER G ET AL: "Nanotiterplates for combinatorial chemistry.", JOURNAL OF BIOTECHNOLOGY. NETHERLANDS DEC 2001, vol. 82, no. 2, December 2001 (2001-12-01), pages 137 - 159, XP002250641, ISSN: 0168-1656 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9200245B2 (en) 2003-06-26 2015-12-01 Seng Enterprises Ltd. Multiwell plate
US8187894B2 (en) 2003-09-30 2012-05-29 Alcatel Lucent Method and apparatus for controlling the flow resistance of a fluid on nanostructured or microstructured surfaces
US9145540B1 (en) 2007-11-15 2015-09-29 Seng Enterprises Ltd. Device for the study of living cells

Also Published As

Publication number Publication date
AU2002363895A1 (en) 2003-07-15
AU2002363895A8 (en) 2003-07-15
WO2003056330A2 (de) 2003-07-10

Similar Documents

Publication Publication Date Title
WO2003056330A3 (de) Zellsortiersystem zur grössenabhängigen sortierung oder separation von in einer strömenden flüssigkeit suspendierten zellen
EP1625203A4 (de) Effiziente sortierung haploider zellen für durchflusszytometer-systeme
WO2003006133A3 (en) Microfluidic devices and systems for separating components of a mixture
CA2438077A1 (en) Cyclonic separating apparatus
AU2001269896A1 (en) Systems and methods for cell subpopulation analysis
WO2002029600A3 (de) Zellenarordnung mit segmentierterwischenzellstruktur
DE50015274D1 (de) Einrichtung zum handhaben von flüssigkeitsproben und herstellungsverfahren sowie system zum handhaben von flüssigkeitsproben
WO2002081085A3 (en) Well-plate with microfluidic structures
WO2004029208A3 (en) Cell separation compositions and methods
MXPA03007276A (es) Procedimiento y dispositivo para la fabricacion de placas delgadas de una pelicula de sustancias activas.
WO2003033868A8 (en) Single well development system
WO2003000888A3 (de) Verfahren zur aufreinigung von bakterienzellen und zellbestandteilen
WO2003093674A3 (en) Electroosmotic flow systems
WO2003057715A3 (en) Novel ligand involved in the transmigration of leukocytes .
EP1640716A4 (de) Blutzellentrennungsmembran und blutrückhaltungswerkzeug dies beinhaltend
IS5668A (is) Aðferð til að skilja segulmagnaðar agnir úr vökvasem þær eru í, skiljukerfi og skilja
DK1348124T3 (da) Identifikation af individuelle celler under kinetiske assays
WO2002029400A3 (en) Apparatuses and methods for field flow fractionation of particles using acoustic and other forces
WO2003056335A3 (de) Mikrovertiefungsarray zur grössenabhängigen sortierung oder separation von in einer strömenden flüssigkeit suspendierten zellen und verfahren zur untersuchung der funktionellen aktivität von einzelzellen
DE69816068D1 (de) Vorrichtung zur abtrennung von feststoffen aus einer flüssigkeit
DE502004003628D1 (de) Substratstapel, insbesondere für die kryokonservierung biologischer proben
WO2002081045A3 (en) Meniscus membranes for separations
CA2436354A1 (en) Separator and method of manufacturing a separator
ATE467584T1 (de) Vorrichtung zum vereinzeln und überführen von gegenständen, sowie überführungseinrichtung für verwendung in dieser vorrichtung
AU2002250018A1 (en) Coalescing and separating arrangements, systems, and methods for liquid mixtures

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AU JP US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE SI TR

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP