AU2001269896A1 - Systems and methods for cell subpopulation analysis - Google Patents

Systems and methods for cell subpopulation analysis

Info

Publication number
AU2001269896A1
AU2001269896A1 AU2001269896A AU6989601A AU2001269896A1 AU 2001269896 A1 AU2001269896 A1 AU 2001269896A1 AU 2001269896 A AU2001269896 A AU 2001269896A AU 6989601 A AU6989601 A AU 6989601A AU 2001269896 A1 AU2001269896 A1 AU 2001269896A1
Authority
AU
Australia
Prior art keywords
cells
separator
dielectrophoretic
force
systems
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001269896A
Inventor
Frederick F. Becker
Peter R. C. Gascoyne
Jody Vykoukal
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Texas System
Original Assignee
University of Texas System
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Texas System filed Critical University of Texas System
Publication of AU2001269896A1 publication Critical patent/AU2001269896A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/028—Non-uniform field separators using travelling electric fields, i.e. travelling wave dielectrophoresis [TWD]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44786—Apparatus specially adapted therefor of the magneto-electrophoresis type
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/0005—Field flow fractionation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1023—Microstructural devices for non-optical measurement
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G01N2015/0288—Sorting the particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1028—Sorting particles

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Electrostatic Separation (AREA)

Abstract

Systems, apparatuses and methods for cell isolation and analysis. Cells are introduced into a dielectrophoretic prefilter including one or more trapping electrodes configured to trap at least a portion of the cells with a dielectrophoretic force. The cells trapped from the prefilter are directed into a dielectrophoretic field-flow fractionation separator coupled to the prefilter. The cells are discriminated by balancing a dielectrophoretic force (and optionally a magnetophoretic force) with a gravitational force to displace the cells to positions within a velocity profile in the separator. At least a portion of the cells are trapped as a function of the cells' time of emergence from the separator with two or more spiral electrode segments coupled to the separator.
AU2001269896A 2000-06-14 2001-06-14 Systems and methods for cell subpopulation analysis Abandoned AU2001269896A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US21151400P 2000-06-14 2000-06-14
US60/211,514 2000-06-14
PCT/US2001/019479 WO2001096025A2 (en) 2000-06-14 2001-06-14 Systems and methods for cell subpopulation analysis

Publications (1)

Publication Number Publication Date
AU2001269896A1 true AU2001269896A1 (en) 2001-12-24

Family

ID=22787235

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001269896A Abandoned AU2001269896A1 (en) 2000-06-14 2001-06-14 Systems and methods for cell subpopulation analysis

Country Status (8)

Country Link
US (1) US6790330B2 (en)
EP (1) EP1328803B1 (en)
JP (1) JP2004532968A (en)
AT (1) ATE304174T1 (en)
AU (1) AU2001269896A1 (en)
CA (1) CA2413194A1 (en)
DE (1) DE60113287D1 (en)
WO (1) WO2001096025A2 (en)

Families Citing this family (128)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6048734A (en) 1995-09-15 2000-04-11 The Regents Of The University Of Michigan Thermal microvalves in a fluid flow method
AU2001271330A1 (en) * 2000-06-14 2001-12-24 Board Of Regents, The University Of Texas System Method and apparatus for combined magnetophoretic and dielectrophoretic manipulation of analyte mixtures
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US6852287B2 (en) 2001-09-12 2005-02-08 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US7323140B2 (en) 2001-03-28 2008-01-29 Handylab, Inc. Moving microdroplets in a microfluidic device
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
CA2467587A1 (en) * 2001-11-30 2003-06-12 Fluidigm Corporation Microfluidic device and methods of using same
US20030209438A1 (en) * 2002-05-09 2003-11-13 Vincent Bressler Particle characterization using gravity in opposition to an induced force
US8349276B2 (en) 2002-09-24 2013-01-08 Duke University Apparatuses and methods for manipulating droplets on a printed circuit board
US7329545B2 (en) * 2002-09-24 2008-02-12 Duke University Methods for sampling a liquid flow
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
US8895298B2 (en) * 2002-09-27 2014-11-25 The General Hospital Corporation Microfluidic device for cell separation and uses thereof
US8871446B2 (en) 2002-10-02 2014-10-28 California Institute Of Technology Microfluidic nucleic acid analysis
US7105081B2 (en) * 2002-12-20 2006-09-12 Board Of Regents, The University Of Texas System Methods and apparatus for electrosmear analysis
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
DE10320870A1 (en) * 2003-05-09 2004-12-09 Evotec Technologies Gmbh Particle injector for a cell sorter
US7169282B2 (en) * 2003-05-13 2007-01-30 Aura Biosystems Inc. Dielectrophoresis apparatus
EP1654066B1 (en) 2003-07-31 2014-11-12 Handylab, Inc. Processing particle-containing samples
ES2239886B1 (en) * 2003-11-05 2006-12-16 Universidad De Barcelona METHOD AND APPLIANCE FOR THE DETERMINATION OF CELLULAR VIABILITY.
JP5344817B2 (en) 2004-05-03 2013-11-20 ハンディーラブ インコーポレイテッド Processing of samples containing polynucleotides
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
EP1765501A1 (en) * 2004-05-28 2007-03-28 Board of Regents, The University of Texas System Programmable fluidic processors
US20060051265A1 (en) * 2004-09-08 2006-03-09 Health Research, Inc. Apparatus and method for sorting microstructures in a fluid medium
US7592139B2 (en) 2004-09-24 2009-09-22 Sandia National Laboratories High temperature flow-through device for rapid solubilization and analysis
US20060091015A1 (en) * 2004-11-01 2006-05-04 Applera Corporation Surface modification for non-specific adsorption of biological material
US20060177815A1 (en) * 2004-11-29 2006-08-10 The Regents Of The University Of California Dielectrophoretic particle sorter
WO2006063625A1 (en) * 2004-12-17 2006-06-22 Agilent Technologies, Inc. Fractionation using electro elution
US20070196820A1 (en) 2005-04-05 2007-08-23 Ravi Kapur Devices and methods for enrichment and alteration of cells and other particles
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
EP1764418B1 (en) * 2005-09-14 2012-08-22 STMicroelectronics Srl Method and device for the treatment of biological samples using dielectrophoresis
US8734003B2 (en) 2005-09-15 2014-05-27 Alcatel Lucent Micro-chemical mixing
US20070059213A1 (en) * 2005-09-15 2007-03-15 Lucent Technologies Inc. Heat-induced transitions on a structured surface
US8721161B2 (en) * 2005-09-15 2014-05-13 Alcatel Lucent Fluid oscillations on structured surfaces
WO2007102839A2 (en) * 2005-10-27 2007-09-13 Applera Corporation Optoelectronic separation of biomolecules
WO2007050143A2 (en) * 2005-10-27 2007-05-03 Applera Corporation Surface modification in a manipulation chamber
KR101157175B1 (en) * 2005-12-14 2012-07-03 삼성전자주식회사 Microfluidic device and method for concentration and lysis of cells or viruses
US8262886B2 (en) * 2006-02-10 2012-09-11 Kochi University Of Technology Apparatus for analyzing characteristics of particulate with dielectrophoresis of particulate by applying angle-modulated wave and method for the same
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
DK3088083T3 (en) 2006-03-24 2018-11-26 Handylab Inc Method of carrying out PCR down a multi-track cartridge
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US8088616B2 (en) 2006-03-24 2012-01-03 Handylab, Inc. Heater unit for microfluidic diagnostic system
WO2007115815A1 (en) * 2006-04-07 2007-10-18 Pauwels, Rudi Method and apparatus for labelling, reading and sorting of microcarriers
WO2007129383A1 (en) * 2006-05-01 2007-11-15 Shimadzu Corporation Device for measuring total organic carbon
US20100216657A1 (en) * 2006-05-16 2010-08-26 Arcxis Biotechnologies, Inc. Pcr-free sample preparation and detection systems for high speed biologic analysis and identification
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
US8372584B2 (en) 2006-06-14 2013-02-12 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US8656949B2 (en) 2006-08-15 2014-02-25 University Of Maryland College Park Microfluidic devices and methods of fabrication
ATE472372T1 (en) * 2006-10-26 2010-07-15 Imec HANDLING MAGNETIC OR MAGNETIZABLE OBJECTS USING COMBINED MAGNETOPHORESIS AND DIELECTROPHORESIS
US8709787B2 (en) 2006-11-14 2014-04-29 Handylab, Inc. Microfluidic cartridge and method of using same
WO2008060604A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US8702945B2 (en) * 2007-05-18 2014-04-22 University Of Washington Time-varying flows for microfluidic particle separation
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US20090136385A1 (en) 2007-07-13 2009-05-28 Handylab, Inc. Reagent Tube
ES2877602T3 (en) 2007-07-13 2021-11-17 Handylab Inc Polynucleotide Capture Materials and Procedures for Using Them
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
USD621060S1 (en) 2008-07-14 2010-08-03 Handylab, Inc. Microfluidic cartridge
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8268246B2 (en) 2007-08-09 2012-09-18 Advanced Liquid Logic Inc PCB droplet actuator fabrication
EP2042239A1 (en) * 2007-09-10 2009-04-01 Koninklijke Philips Electronics N.V. Dielectrophoretic device and method for cell membrane studies
CA2704216A1 (en) * 2007-10-29 2009-05-07 James F. Leary Hybrid microfluidic spr and molecular imaging device
US20090137925A1 (en) * 2007-11-23 2009-05-28 Divya Cantor Impedance Spectroscopy Cervix Scanning Apparatus and Method
CA2720324C (en) * 2008-04-03 2016-08-23 The Regents Of The University Of California Ex-vivo multi-dimensional system for the separation and isolation of cells, vesicles, nanoparticles and biomarkers
USD618820S1 (en) 2008-07-11 2010-06-29 Handylab, Inc. Reagent holder
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
CN102197308A (en) * 2008-10-31 2011-09-21 皇家飞利浦电子股份有限公司 Biosensor with multi-chamber cartridge
US8162149B1 (en) 2009-01-21 2012-04-24 Sandia Corporation Particle sorter comprising a fluid displacer in a closed-loop fluid circuit
KR101273453B1 (en) * 2010-05-20 2013-06-11 고려대학교 산학협력단 Quantitative Analysis Using Minute Tube with Accumulated Enzyme
US8163182B2 (en) * 2010-05-28 2012-04-24 Wyatt Technology Corporation Compartmentalized field flow fractionation
US9322804B2 (en) * 2010-11-29 2016-04-26 President And Fellows Of Harvard College Quality control of diamagnetic materials using magnetic levitation
US8459464B2 (en) * 2011-03-18 2013-06-11 Fred C. Senftleber Apparatus and method for sedimentation field-flow fractionation
WO2012142516A1 (en) 2011-04-15 2012-10-18 Becton, Dickinson And Company Scanning real-time microfluidic thermo-cycler and methods for synchronized thermocycling and scanning optical detection
DE102011050254A1 (en) * 2011-05-10 2012-11-15 Technische Universität Dortmund Process for the separation of polarizable bioparticles
US9174216B2 (en) 2013-03-13 2015-11-03 DeNovo Science, Inc. System for capturing and analyzing cells
US9404864B2 (en) 2013-03-13 2016-08-02 Denovo Sciences, Inc. System for imaging captured cells
ES2797448T3 (en) 2011-08-01 2020-12-02 Bio Rad Laboratories Cell capture system
US10466160B2 (en) 2011-08-01 2019-11-05 Celsee Diagnostics, Inc. System and method for retrieving and analyzing particles
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
ES2825905T3 (en) 2011-09-30 2021-05-17 Becton Dickinson Co Unified test strip
US11198126B2 (en) 2011-10-31 2021-12-14 Fluid-Screen, Inc. Apparatus for pathogen detection
EP2773892B1 (en) 2011-11-04 2020-10-07 Handylab, Inc. Polynucleotide sample preparation device
BR112014018995B1 (en) 2012-02-03 2021-01-19 Becton, Dickson And Company systems to perform automated testing
US8932815B2 (en) 2012-04-16 2015-01-13 Biological Dynamics, Inc. Nucleic acid sample preparation
EP2839035B1 (en) 2012-04-16 2020-11-25 Biological Dynamics, Inc. Nucleic acid sample preparation
CN104540594B (en) 2012-06-25 2019-07-02 通用医疗公司 Classified using high-gradient magnetic field to particle
EP2906953B1 (en) * 2012-10-12 2017-12-06 HI-STEM gGmbH Im Deutschen Krebsforschungszentrum DKFZ Novel biomarkers for sub-typing pancreatic ductal adenocarcinoma
US9752181B2 (en) 2013-01-26 2017-09-05 Denovo Sciences, Inc. System and method for capturing and analyzing cells
US9809810B2 (en) 2013-02-05 2017-11-07 Ohio State Innovation Foundation Galvanotaxis assay for quantitative assessment of the metastatic potential of cancer cells
WO2014123949A1 (en) 2013-02-05 2014-08-14 Ohio State Innovation Foundation A non-contact method for accelerating wound healing
US9885031B2 (en) 2013-02-05 2018-02-06 Ohio State Innovation Foundation Galvanotaxis assay for quantitative assessment of the metastatic potential of cancer cells
US9707562B2 (en) 2013-03-13 2017-07-18 Denovo Sciences, Inc. System for capturing and analyzing cells
EP2781906B1 (en) 2013-03-19 2021-08-18 IMEC vzw Method for identifying cells
US9856535B2 (en) 2013-05-31 2018-01-02 Denovo Sciences, Inc. System for isolating cells
US10391490B2 (en) 2013-05-31 2019-08-27 Celsee Diagnostics, Inc. System and method for isolating and analyzing cells
US9511368B2 (en) * 2013-08-29 2016-12-06 The Industry & Academic Cooperation In Chungnam National University (Iac) Transporting, trapping and escaping manipulation device for magnetic bead biomaterial comprising micro-magnetophoretic circuit
MX2016013216A (en) 2014-04-08 2017-05-01 Biological dynamics inc Improved devices for separation of biological materials.
WO2016094715A2 (en) * 2014-12-10 2016-06-16 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
DE102015202667A1 (en) * 2015-02-13 2016-08-18 Postnova Analytics Gmbh Device for field flow fractionation
TWI756199B (en) 2016-01-15 2022-03-01 美商伯克利之光生命科技公司 Methods of producing patient-specific anti-cancer therapeutics and methods of treatment therefor
JP6441838B2 (en) 2016-01-29 2018-12-19 株式会社Ａｆｉテクノロジー Analysis device and separation device
JP6273467B2 (en) * 2016-02-08 2018-02-07 パナソニックＩｐマネジメント株式会社 Detection of analyte by dielectrophoresis
CA3018900A1 (en) 2016-03-24 2017-09-28 Biological Dynamics, Inc. Disposable fluidic cartridge and components
EP3437727B1 (en) * 2016-03-30 2021-12-01 Sharp Life Science (EU) Limited Microfluidic device
EP3442711B1 (en) 2016-04-15 2020-12-09 Fluid-Screen, Inc. Analyte detection methods and apparatus using dielectrophoresis and electroosmosis
US11874268B2 (en) * 2016-06-12 2024-01-16 Nanohesgarsazan Salamat Arya Ncubation Center For Equipment And Devices Method and system for metastasis diagnosis and prognosis
WO2018187610A1 (en) 2017-04-05 2018-10-11 The Regents Of The University Of California A device for continuous focusing and rotation of biological cells and its use for high throughput electrorotation flow cytometry
EP3622084A4 (en) 2017-05-08 2021-02-17 Biological Dynamics, Inc. Methods and systems for analyte information processing
CN111295245B (en) 2017-08-29 2021-03-16 伯乐实验室有限公司 Systems and methods for separating and analyzing cells
CN116121340A (en) 2017-12-19 2023-05-16 生物动力学公司 Method and device for detecting multiple analytes from biological samples
MX2020009199A (en) 2018-03-30 2021-01-20 Idexx Lab Inc Quality control for point-of-care diagnostic systems.
CN117065932A (en) 2018-04-02 2023-11-17 生物动力学公司 Dielectric material
US10633693B1 (en) 2019-04-16 2020-04-28 Celsee Diagnostics, Inc. System and method for leakage control in a particle capture system
CA3138881A1 (en) 2019-05-07 2020-11-12 Bio-Rad Laboratories, Inc. System and method for automated single cell processing
US11273439B2 (en) 2019-05-07 2022-03-15 Bio-Rad Laboratories, Inc. System and method for target material retrieval from microwells
KR20220033484A (en) 2019-06-14 2022-03-16 바이오 래드 래버러토리스 인코오포레이티드 Systems and Methods for Automated Single Cell Processing and Analysis
US11971392B2 (en) * 2019-06-25 2024-04-30 Wyatt Technology, Llc Sealing structure for a field flow fractionator
JP7215366B2 (en) * 2019-07-17 2023-01-31 株式会社島津製作所 Asymmetric flow flow field fractionator
WO2021067650A1 (en) * 2019-10-02 2021-04-08 The Regents Of The University Of California Timed release analysis of cells incubated in microenvironment nodes
WO2021097035A1 (en) 2019-11-13 2021-05-20 Fluid-Screen, Inc. An apparatus and methods to rapidly detect, separate, purify, and quantify various viruses from cells, cultured medium and other fluids
CA3161454A1 (en) 2019-11-13 2021-05-20 Fluid-Screen, Inc. Methods and apparatus for detection of bacteria in a sample using dielectrophoresis
DE102020202274A1 (en) 2020-02-21 2021-08-26 Robert Bosch Gesellschaft mit beschränkter Haftung Microfluidic channel element for improved separation of target particles from non-target particles within a medium on the basis of dielectrophoresis
US11504719B2 (en) 2020-03-12 2022-11-22 Bio-Rad Laboratories, Inc. System and method for receiving and delivering a fluid for sample processing
EP4089394A1 (en) * 2021-05-11 2022-11-16 Ceidos SA Cell culture monitoring system

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4001102A (en) 1973-04-06 1977-01-04 The Carborundum Company Process for generating periodic non-uniform electric field, and for removing polarizable particulate material from fluid, using ferroelectric apparatus
SU474723A1 (en) 1973-06-04 1975-06-25 Институт биологической физики АН СССР Disperse Particle Separation Device
US4214981A (en) * 1978-10-23 1980-07-29 University Of Utah Steric field-flow fractionation
US4326934A (en) 1979-12-31 1982-04-27 Pohl Herbert A Continuous dielectrophoretic cell classification method
US4440638A (en) 1982-02-16 1984-04-03 U.T. Board Of Regents Surface field-effect device for manipulation of charged species
JP2720161B2 (en) 1988-02-01 1998-02-25 株式会社アドバンス Cell deformability measuring device
US4966918A (en) 1989-01-27 1990-10-30 Sloan-Kettering Institute For Cancer Research Derivatives of chryosphanol
US5344535A (en) * 1989-11-27 1994-09-06 British Technology Group Limited Dielectrophoretic characterization of micro-organisms and other particles
GB9002092D0 (en) 1990-01-30 1990-03-28 P & B Sciences Ltd Manipulation of solid,semi-solid or liquid materials
DE4143573C2 (en) 1991-08-19 1996-07-04 Fraunhofer Ges Forschung Device for separating mixtures of microscopic dielectric particles suspended in a liquid or a gel
JP3097932B2 (en) 1991-11-05 2000-10-10 株式会社アドバンス Electrostatic chromatography equipment
US6017696A (en) 1993-11-01 2000-01-25 Nanogen, Inc. Methods for electronic stringency control for molecular biological analysis and diagnostics
JP3200066B2 (en) 1992-02-08 2001-08-20 ジェネラ テクノロジィーズ リミテッド Analysis method
GB9208357D0 (en) 1992-04-16 1992-06-03 British Tech Group Apparatus for separating a mixture
JP3282679B2 (en) 1992-07-01 2002-05-20 株式会社アドバンス Methods for DNA sequencing
US5489506A (en) 1992-10-26 1996-02-06 Biolife Systems, Inc. Dielectrophoretic cell stream sorter
GB9301122D0 (en) 1993-01-21 1993-03-10 Scient Generics Ltd Method of analysis/separation
GB9306729D0 (en) 1993-03-31 1993-05-26 British Tech Group Improvements in separators
US6129828A (en) 1996-09-06 2000-10-10 Nanogen, Inc. Apparatus and methods for active biological sample preparation
US6225059B1 (en) 1993-11-01 2001-05-01 Nanogen, Inc. Advanced active electronic devices including collection electrodes for molecular biological analysis and diagnostics
US6099803A (en) 1994-07-07 2000-08-08 Nanogen, Inc. Advanced active electronic devices for molecular biological analysis and diagnostics
US5965452A (en) 1996-07-09 1999-10-12 Nanogen, Inc. Multiplexed active biologic array
US5436243A (en) 1993-11-17 1995-07-25 Research Triangle Institute Duke University Aminoanthraquinone derivatives to combat multidrug resistance
DE4400955C2 (en) 1993-12-23 1999-04-01 Fraunhofer Ges Forschung Adhesion-controllable surface structure
US5571401A (en) 1995-03-27 1996-11-05 California Institute Of Technology Sensor arrays for detecting analytes in fluids
US5788833A (en) 1995-03-27 1998-08-04 California Institute Of Technology Sensors for detecting analytes in fluids
GB9507125D0 (en) 1995-04-06 1995-05-31 Scient Generics Ltd Travelling wave electrodes
DE19515524C2 (en) 1995-04-27 1999-09-09 Private Uni Witten Herdecke Gm Method and device for the continuous detection of at least one substance in a gaseous or liquid mixture by means of a sensor electrode
US5993630A (en) 1996-01-31 1999-11-30 Board Of Regents The University Of Texas System Method and apparatus for fractionation using conventional dielectrophoresis and field flow fractionation
US5888370A (en) 1996-02-23 1999-03-30 Board Of Regents, The University Of Texas System Method and apparatus for fractionation using generalized dielectrophoresis and field flow fractionation
US5683569A (en) 1996-02-28 1997-11-04 Motorola, Inc. Method of sensing a chemical and sensor therefor
GB9605628D0 (en) 1996-03-18 1996-05-22 Univ North Wales Apparatus for carrying out reactions
US5858192A (en) * 1996-10-18 1999-01-12 Board Of Regents, The University Of Texas System Method and apparatus for manipulation using spiral electrodes
GB2356162B (en) * 1998-05-29 2002-03-06 Ind Res Ltd Method and apparatus for concentrating and/or positioning particles or cells
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
IT1309430B1 (en) 1999-05-18 2002-01-23 Guerrieri Roberto METHOD AND APPARATUS FOR HANDLING PARTICLES BY MEANS OF ELECTROPHORESIS

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EP1328803A2 (en) 2003-07-23
US20020036142A1 (en) 2002-03-28
DE60113287D1 (en) 2005-10-13
US6790330B2 (en) 2004-09-14
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EP1328803B1 (en) 2005-09-07
CA2413194A1 (en) 2001-12-20

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