BRPI0408852A - processo para a coloração de espermatozóide - Google Patents

processo para a coloração de espermatozóide

Info

Publication number
BRPI0408852A
BRPI0408852A BRPI0408852-2A BRPI0408852A BRPI0408852A BR PI0408852 A BRPI0408852 A BR PI0408852A BR PI0408852 A BRPI0408852 A BR PI0408852A BR PI0408852 A BRPI0408852 A BR PI0408852A
Authority
BR
Brazil
Prior art keywords
sperm
coloring
cells
sperm cells
involve
Prior art date
Application number
BRPI0408852-2A
Other languages
English (en)
Inventor
Muhammad Anzar
Cindy L Ludwig
Jeffrey A Graham
Jeannet A Glaenzer
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of BRPI0408852A publication Critical patent/BRPI0408852A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives

Abstract

"PROCESSO PARA A COLORAçãO DE ESPERMATOZóIDE". A presente invenção refere-se a células de espermatozóide que são coradas de acordo com processos que envolvem a combinação de células de espermatozóide com um corante seletivo para DNA fluorescente em uma temperatura elevada em excesso de cerca de 40EC. Os métodos possibilitam uma redução do tempo de coloração. As células depois disso podem ser eficientemente classificadas de acordo com métodos comuns de separação, inclusive citometria de fluxo.
BRPI0408852-2A 2003-03-28 2004-03-29 processo para a coloração de espermatozóide BRPI0408852A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US45873103P 2003-03-28 2003-03-28
PCT/US2004/009903 WO2004087177A1 (en) 2003-03-28 2004-03-29 Process for the staining of sperm

Publications (1)

Publication Number Publication Date
BRPI0408852A true BRPI0408852A (pt) 2006-04-04

Family

ID=33131822

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0408852-2A BRPI0408852A (pt) 2003-03-28 2004-03-29 processo para a coloração de espermatozóide

Country Status (14)

Country Link
US (1) US7335507B2 (pt)
EP (1) EP1608382B1 (pt)
JP (1) JP2006525019A (pt)
CN (1) CN100379429C (pt)
AT (1) ATE369867T1 (pt)
AU (1) AU2004226343B2 (pt)
BR (1) BRPI0408852A (pt)
CA (1) CA2519989A1 (pt)
DE (1) DE602004008241T2 (pt)
MX (1) MXPA05010478A (pt)
NZ (1) NZ542688A (pt)
PL (1) PL1608382T3 (pt)
WO (1) WO2004087177A1 (pt)
ZA (1) ZA200507823B (pt)

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US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
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BRPI0313476B1 (pt) * 2002-08-15 2015-06-23 Xy Llc Citômetro de fluxo de alta resolução
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JP2006525019A (ja) 2003-03-28 2006-11-09 モンサント テクノロジー エルエルシー 精子の染色方法
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US20110236923A1 (en) * 2010-03-23 2011-09-29 Genetics & Ivf Institute Method for staining and sorting of a small volume of sperm
WO2011123166A2 (en) * 2010-04-01 2011-10-06 Inguran, Llc Methods and systems for reducing dna fragmentation in a processed sperm sample
CN101793641B (zh) * 2010-04-14 2011-09-28 内蒙古蒙牛繁育生物技术股份有限公司 一种用于奶牛精子分离的荧光染色方法
CN102095675B (zh) * 2010-11-23 2012-05-23 内蒙古赛科星繁育生物技术股份有限公司 混合奶牛性控冻精产品鉴定和检测方法
BR112013030815B1 (pt) 2011-06-01 2019-02-19 Inguran, Llc Composições e método para aprimorar a qualidade do esperma processado
US9781919B2 (en) 2011-06-01 2017-10-10 Inguran, Llc Compositions and methods for improving the quality of processed sperm
WO2013049631A1 (en) 2011-09-30 2013-04-04 Inguran, Llc Sperm staining and sorting methods
US9888990B2 (en) 2012-06-06 2018-02-13 Inguran, Llc Methods for use of sex sorted semen to improve genetic management in swine
US9433195B2 (en) 2012-06-06 2016-09-06 Inguran, Llc Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells
CA2886782C (en) 2012-10-05 2020-03-10 Inguran, Llc High efficiency methods of sex sorting sperm
US10620213B2 (en) 2012-10-05 2020-04-14 Inguran, Llc High pressure sperm sorting and flow cytometer methods
CN103344543B (zh) * 2013-06-28 2016-07-06 浙江星博生物科技股份有限公司 一种基于流式细胞术的精子线粒体膜电位检测试剂
CN103335934A (zh) * 2013-06-28 2013-10-02 浙江星博生物科技有限公司 一种基于流式细胞术的精子活率检测试剂
CN103555662B (zh) * 2013-10-31 2015-09-16 大连金弘基种畜有限公司 将四溴化1,1,2,2-四-[4-(2-三乙基乙氧基胺基)]苯乙烯应用于精子分离
CN104749144A (zh) * 2013-12-31 2015-07-01 深圳迈瑞生物医疗电子股份有限公司 血细胞检测试剂及血细胞处理方法和识别方法
EP3772937A4 (en) * 2018-04-09 2021-12-29 Inguran, LLC Improved sperm nuclei and methods of their manufacture and use
US10603075B1 (en) 2018-11-30 2020-03-31 Ohana Biosciences, Inc. Compositions and methods for enhancing sperm function

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JP2006525019A (ja) 2003-03-28 2006-11-09 モンサント テクノロジー エルエルシー 精子の染色方法
EP2306174B1 (en) 2003-03-28 2016-05-11 Inguran, LLC Flow cytometry nozzle for orienting particles and corresponding method

Also Published As

Publication number Publication date
EP1608382B1 (en) 2007-08-15
CA2519989A1 (en) 2004-10-14
CN1795004A (zh) 2006-06-28
AU2004226343B2 (en) 2008-10-09
WO2004087177A1 (en) 2004-10-14
ZA200507823B (en) 2008-03-26
US20050003472A1 (en) 2005-01-06
JP2006525019A (ja) 2006-11-09
US7335507B2 (en) 2008-02-26
CN100379429C (zh) 2008-04-09
MXPA05010478A (es) 2005-12-15
DE602004008241T2 (de) 2008-05-08
ATE369867T1 (de) 2007-09-15
EP1608382A1 (en) 2005-12-28
NZ542688A (en) 2008-06-30
AU2004226343A1 (en) 2004-10-14
DE602004008241D1 (de) 2007-09-27
PL1608382T3 (pl) 2008-03-31

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Legal Events

Date Code Title Description
B25A Requested transfer of rights approved

Owner name: INGURAN LLC (US)

Free format text: TRANSFERIDO DE: MONSANTO TECHNOLOGY LLC

B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE AS 6A, 7A E 8A ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2159 DE 22/05/2012.

B15K Others concerning applications: alteration of classification

Ipc: C12N 5/071 (2010.01)