DE602004008241D1 - Verfahren zum anfärben von sperma - Google Patents

Verfahren zum anfärben von sperma

Info

Publication number
DE602004008241D1
DE602004008241D1 DE602004008241T DE602004008241T DE602004008241D1 DE 602004008241 D1 DE602004008241 D1 DE 602004008241D1 DE 602004008241 T DE602004008241 T DE 602004008241T DE 602004008241 T DE602004008241 T DE 602004008241T DE 602004008241 D1 DE602004008241 D1 DE 602004008241D1
Authority
DE
Germany
Prior art keywords
sperm
coloring
cells
sperm cells
involve
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE602004008241T
Other languages
English (en)
Other versions
DE602004008241T2 (de
Inventor
Muhammad Anzar
Cindy L Ludwig
Jeffrey A Graham
Jeannet A Glaenzer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Inguran LLC
Original Assignee
Monsanto Technology LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology LLC filed Critical Monsanto Technology LLC
Publication of DE602004008241D1 publication Critical patent/DE602004008241D1/de
Application granted granted Critical
Publication of DE602004008241T2 publication Critical patent/DE602004008241T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Reproductive Health (AREA)
  • Endocrinology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
DE602004008241T 2003-03-28 2004-03-29 Verfahren zum anfärben von sperma Expired - Lifetime DE602004008241T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US45873103P 2003-03-28 2003-03-28
US458731P 2003-03-28
PCT/US2004/009903 WO2004087177A1 (en) 2003-03-28 2004-03-29 Process for the staining of sperm

Publications (2)

Publication Number Publication Date
DE602004008241D1 true DE602004008241D1 (de) 2007-09-27
DE602004008241T2 DE602004008241T2 (de) 2008-05-08

Family

ID=33131822

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602004008241T Expired - Lifetime DE602004008241T2 (de) 2003-03-28 2004-03-29 Verfahren zum anfärben von sperma

Country Status (14)

Country Link
US (1) US7335507B2 (de)
EP (1) EP1608382B1 (de)
JP (1) JP2006525019A (de)
CN (1) CN100379429C (de)
AT (1) ATE369867T1 (de)
AU (1) AU2004226343B2 (de)
BR (1) BRPI0408852A (de)
CA (1) CA2519989A1 (de)
DE (1) DE602004008241T2 (de)
MX (1) MXPA05010478A (de)
NZ (1) NZ542688A (de)
PL (1) PL1608382T3 (de)
WO (1) WO2004087177A1 (de)
ZA (1) ZA200507823B (de)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU752985B2 (en) 1997-01-31 2002-10-03 Xy, Llc. Optical apparatus
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US6071689A (en) * 1997-12-31 2000-06-06 Xy, Inc. System for improving yield of sexed embryos in mammals
JP2002521043A (ja) 1998-07-30 2002-07-16 エックスワイ，インコーポレイテッド 非外科的人工授精のためのウマシステム
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
DK2258172T3 (en) * 2000-05-09 2017-08-07 Xy Llc Flow cytometer for differentiation of X-chromosome-bearing and Y-chromosome-bearing populations of spermatozoa
EP1294293A4 (de) * 2000-06-12 2009-11-04 Xy Inc Integriertes herdenmanagementsystem unter verwendung von isolierten populationen von x- und y-chromosomen-tragenden spermatozoen
US20040031071A1 (en) * 2000-10-05 2004-02-12 Xy, Inc. System of hysteroscopic insemination of mares
WO2002043574A2 (en) 2000-11-29 2002-06-06 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
CA2532376C (en) 2002-08-01 2015-05-12 Colorado State University Research Foundation Low pressure sperm cell separation system
MXPA05001654A (es) * 2002-08-15 2005-10-18 Xy Inc Citometro de flujo de alta resolucion.
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
JP2006525019A (ja) 2003-03-28 2006-11-09 モンサント テクノロジー エルエルシー 精子の染色方法
CA3074799C (en) 2003-03-28 2022-12-06 Inguran, Llc Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm
US20060263829A1 (en) 2003-05-15 2006-11-23 Evans Kenneth M Efficient haploid cell sorting flow cytometer systems
AU2005228046B2 (en) * 2004-03-29 2011-01-20 Inguran, Llc Use of a composition which regulates regulates oxidation/reduction reactions intracellularly and/or extracellularly in a staining or sorting process of spermatozoa
US7892725B2 (en) * 2004-03-29 2011-02-22 Inguran, Llc Process for storing a sperm dispersion
CA2574499C (en) * 2004-07-22 2016-11-29 Monsanto Technology Llc Process for enriching a population of sperm cells
US7355696B2 (en) 2005-02-01 2008-04-08 Arryx, Inc Method and apparatus for sorting cells
KR100949923B1 (ko) * 2008-04-21 2010-03-30 중앙대학교 산학협력단 번식능력이 불량한 수퇘지 선별법
US20110236923A1 (en) * 2010-03-23 2011-09-29 Genetics & Ivf Institute Method for staining and sorting of a small volume of sperm
WO2011123166A2 (en) * 2010-04-01 2011-10-06 Inguran, Llc Methods and systems for reducing dna fragmentation in a processed sperm sample
CN101793641B (zh) * 2010-04-14 2011-09-28 内蒙古蒙牛繁育生物技术股份有限公司 一种用于奶牛精子分离的荧光染色方法
CN102095675B (zh) * 2010-11-23 2012-05-23 内蒙古赛科星繁育生物技术股份有限公司 混合奶牛性控冻精产品鉴定和检测方法
US9781919B2 (en) 2011-06-01 2017-10-10 Inguran, Llc Compositions and methods for improving the quality of processed sperm
WO2012167151A1 (en) 2011-06-01 2012-12-06 Inguran, Llc Compositions and methods for improving the quality of processed sperm
US9140688B2 (en) 2011-09-30 2015-09-22 Inguran, Llc Sperm staining and sorting methods
US9433195B2 (en) 2012-06-06 2016-09-06 Inguran, Llc Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells
US9888990B2 (en) 2012-06-06 2018-02-13 Inguran, Llc Methods for use of sex sorted semen to improve genetic management in swine
US10620213B2 (en) 2012-10-05 2020-04-14 Inguran, Llc High pressure sperm sorting and flow cytometer methods
EP4066637A1 (de) 2012-10-05 2022-10-05 Inguran, LLC Verfahren zur verarbeitung sperma für geschlechtssortierung
CN103335934A (zh) * 2013-06-28 2013-10-02 浙江星博生物科技有限公司 一种基于流式细胞术的精子活率检测试剂
CN103344543B (zh) * 2013-06-28 2016-07-06 浙江星博生物科技股份有限公司 一种基于流式细胞术的精子线粒体膜电位检测试剂
CN103555662B (zh) * 2013-10-31 2015-09-16 大连金弘基种畜有限公司 将四溴化1,1,2,2-四-[4-(2-三乙基乙氧基胺基)]苯乙烯应用于精子分离
CN104749144A (zh) * 2013-12-31 2015-07-01 深圳迈瑞生物医疗电子股份有限公司 血细胞检测试剂及血细胞处理方法和识别方法
EP3772937A4 (de) 2018-04-09 2021-12-29 Inguran, LLC Verbesserte spermienkerne und verfahren zu deren herstellung und verwendung
US10470798B1 (en) 2018-11-30 2019-11-12 Ohana Biosciences, Inc. Methods for promoting fertilization

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3005756A (en) 1958-11-14 1961-10-24 Noland L Van Demark Diluter containing carbon dioxide for preserving semen
US4474875A (en) 1969-04-10 1984-10-02 Wallace Shrimpton Method and means for controlling the sex of mammalian offspring and product therefor
US3791384A (en) 1971-07-15 1974-02-12 Schaumann H Artificial insemination of sows
US4267268A (en) 1979-03-12 1981-05-12 Nelson Jr Robert A Spermatozoa extenders
US4362246A (en) 1980-07-14 1982-12-07 Adair Edwin Lloyd Method of treating collected mammal semen and separating sperm into X Y components
GB2145112B (en) 1983-04-27 1987-02-18 Milk Marketing Board Sorting living spermatozoa
JP2552582B2 (ja) * 1989-05-10 1996-11-13 アメリカ合衆国 子の性の前選択の方法
US5658751A (en) 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5798276A (en) 1995-06-07 1998-08-25 Molecular Probes, Inc. Reactive derivatives of sulforhodamine 101 with enhanced hydrolytic stability
US6040139A (en) 1995-09-19 2000-03-21 Bova; G. Steven Laser cell purification system
AU722176B2 (en) 1995-11-09 2000-07-27 Medical Research Council Use of CYB medium for the transportation and storage of sperm
US5985216A (en) 1997-07-24 1999-11-16 The United States Of America, As Represented By The Secretary Of Agriculture Flow cytometry nozzle for high efficiency cell sorting
EP1028622A1 (de) 1997-09-22 2000-08-23 University Of Guelph Verringerung der empfindlichkeit von sperma gegen abkühlung
US6071689A (en) 1997-12-31 2000-06-06 Xy, Inc. System for improving yield of sexed embryos in mammals
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US6642018B1 (en) 1998-03-27 2003-11-04 Oncosis Llc Method for inducing a response in one or more targeted cells
US6309815B1 (en) 1998-08-07 2001-10-30 University Of Kansas Medical Center Composition and method for preparation, storage and activation of large populations of immotile sperm
WO2000054026A1 (en) 1999-03-05 2000-09-14 Hatting-Ks Determination of sperm concentration and viability for artificial insemination
FR2793495B1 (fr) 1999-05-14 2001-08-10 Imv Technologies Dilueur pour la cryoconservation de spermatozoides de bovins
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
US6263745B1 (en) 1999-12-03 2001-07-24 Xy, Inc. Flow cytometer nozzle and flow cytometer sample handling methods
IT1317724B1 (it) * 2000-01-14 2003-07-15 Istituto Sperimentale Italiano Procedimento per la produzione di embrioni non umani di sessopredeterminato ad alto valore genetico.
DK2258172T3 (en) 2000-05-09 2017-08-07 Xy Llc Flow cytometer for differentiation of X-chromosome-bearing and Y-chromosome-bearing populations of spermatozoa
US7193706B2 (en) 2000-08-02 2007-03-20 Arizona Board Of Regents, Acting On Behalf Of Arizona State University Computer interfaced scanning fluorescence lifetime microscope applied to directed evolution
AU1980202A (en) 2000-11-22 2002-06-03 Pharmacia Corporation Methods and apparatus for producing gender enriched sperm
WO2002043574A2 (en) 2000-11-29 2002-06-06 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
AU2002254173A1 (en) * 2001-03-12 2002-10-08 The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The Unive Oxidation-reduction sensitive green fluorescent protein variants
JP2006525019A (ja) 2003-03-28 2006-11-09 モンサント テクノロジー エルエルシー 精子の染色方法
CA3074799C (en) 2003-03-28 2022-12-06 Inguran, Llc Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm

Also Published As

Publication number Publication date
AU2004226343B2 (en) 2008-10-09
US20050003472A1 (en) 2005-01-06
MXPA05010478A (es) 2005-12-15
ATE369867T1 (de) 2007-09-15
ZA200507823B (en) 2008-03-26
WO2004087177A1 (en) 2004-10-14
AU2004226343A1 (en) 2004-10-14
US7335507B2 (en) 2008-02-26
EP1608382A1 (de) 2005-12-28
PL1608382T3 (pl) 2008-03-31
CN100379429C (zh) 2008-04-09
NZ542688A (en) 2008-06-30
CA2519989A1 (en) 2004-10-14
JP2006525019A (ja) 2006-11-09
DE602004008241T2 (de) 2008-05-08
EP1608382B1 (de) 2007-08-15
BRPI0408852A (pt) 2006-04-04
CN1795004A (zh) 2006-06-28

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Legal Events

Date Code Title Description
8328 Change in the person/name/address of the agent

Representative=s name: KADOR & PARTNER, 80469 MUENCHEN

8327 Change in the person/name/address of the patent owner

Owner name: INGURAN, LLC (N.D.GES.D. STAATES DELAWARE), NA, US

8364 No opposition during term of opposition