ATE498682T1 - Zweidimensionale strangform- und längenabhängige trennung von nukleinsäurefragmenten - Google Patents

Zweidimensionale strangform- und längenabhängige trennung von nukleinsäurefragmenten

Info

Publication number
ATE498682T1
ATE498682T1 AT05781069T AT05781069T ATE498682T1 AT E498682 T1 ATE498682 T1 AT E498682T1 AT 05781069 T AT05781069 T AT 05781069T AT 05781069 T AT05781069 T AT 05781069T AT E498682 T1 ATE498682 T1 AT E498682T1
Authority
AT
Austria
Prior art keywords
electrophoresis
length
nucleic acid
dimension
strandness
Prior art date
Application number
AT05781069T
Other languages
English (en)
Inventor
Gudmundur H Gunnarsson
Hans Guttormur Thormar
Bjarki Gudmundsson
Jon Johannes Jonsson
Original Assignee
Lifeind Ehf
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lifeind Ehf filed Critical Lifeind Ehf
Application granted granted Critical
Publication of ATE498682T1 publication Critical patent/ATE498682T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT05781069T 2004-09-02 2005-09-02 Zweidimensionale strangform- und längenabhängige trennung von nukleinsäurefragmenten ATE498682T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IS7436 2004-09-02
PCT/IS2005/000019 WO2006025074A2 (en) 2004-09-02 2005-09-02 Twodimensional strandness- and length-dependent separation of nucleic acid fragments

Publications (1)

Publication Number Publication Date
ATE498682T1 true ATE498682T1 (de) 2011-03-15

Family

ID=35601867

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05781069T ATE498682T1 (de) 2004-09-02 2005-09-02 Zweidimensionale strangform- und längenabhängige trennung von nukleinsäurefragmenten

Country Status (8)

Country Link
US (1) US8241475B2 (de)
EP (1) EP1791955B1 (de)
JP (1) JP5079509B2 (de)
AT (1) ATE498682T1 (de)
AU (1) AU2005278786A1 (de)
CA (1) CA2614580A1 (de)
DE (1) DE602005026412D1 (de)
WO (1) WO2006025074A2 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120070830A1 (en) * 2010-09-16 2012-03-22 lbis Biosciences, Inc. Stabilization of ozone-labile fluorescent dyes by thiourea
GB201102385D0 (en) 2011-02-10 2011-03-30 Biocule Scotland Ltd Two-dimensional gel electrophoresis apparatus and method
JP6404785B2 (ja) * 2015-08-12 2018-10-17 日本電信電話株式会社 生体分子移動度制御方法および電気泳動槽
JP7305812B2 (ja) * 2016-07-19 2023-07-10 株式会社バイオダイナミクス研究所 長鎖一本鎖ｄｎａを調製する方法
WO2020086366A1 (en) * 2018-10-22 2020-04-30 Merck Sharp & Dohme Corp. Methods for separating large nucleic acids under denatured conditions
CN111855982B (zh) * 2019-04-25 2022-07-22 武汉华大医学检验所有限公司 检测核酸片段长度的方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL8801147A (nl) * 1988-05-02 1989-12-01 Tno Werkwijze voor het detecteren van genetische variatie, en daarvoor geschikte kit.
US6147198A (en) 1988-09-15 2000-11-14 New York University Methods and compositions for the manipulation and characterization of individual nucleic acid molecules
US5074980A (en) * 1989-11-29 1991-12-24 E. I. Du Pont De Nemours And Company Method of characterizing polynucleotides
US5162514A (en) * 1990-05-15 1992-11-10 Board Of Regents, University Of Texas High molecular weight dna compositions for use in electrophoresis of large nucleic acids
US5212299A (en) * 1991-05-10 1993-05-18 Fmc Corporation Glyceryl agarose and borate compositions
CA2245888C (en) * 1996-02-14 2008-12-23 Jaap Goudsmit Isolation and amplification of nucleic acid materials
WO1997030152A1 (en) * 1996-02-14 1997-08-21 Amsterdam Support Diagnostics B.V. Separation of single stranded and double stranded nucleic acid materials
AU2803397A (en) * 1996-04-17 1997-11-07 Ramot University Authority For Applied Research And Industrial Development Ltd. Method and kit for detecting genomic instability
WO1998044156A1 (en) * 1997-03-27 1998-10-08 Life Technologies, Inc. Nucleic acid ladders
DE69842088D1 (de) * 1997-07-15 2011-02-17 Life Technologies Inc Nukleinsäureleitern
US6210931B1 (en) * 1998-11-30 2001-04-03 The United States Of America As Represented By The Secretary Of Agriculture Ribozyme-mediated synthesis of circular RNA
CA2417409A1 (en) * 2000-08-02 2002-02-07 Applera Corporation Methods for isolating one strand of a double-stranded nucleic acid
EP1379864A1 (de) * 2001-04-17 2004-01-14 Nextgen Sciences Ltd Elektrophoretisches trennungssystem
WO2003058230A1 (en) * 2002-01-11 2003-07-17 Ludesi Ab Method and computer program for digital image processing for two-dimensional electrophoresis
DE60321449D1 (de) * 2002-02-25 2008-07-17 Lifeind Ehf Verfahren zur zweidimensionalen konformationsabhängigen trennung nichtzirkulärer nukleinsäuren
CA2486283A1 (en) * 2002-05-17 2004-02-05 Nugen Technologies, Inc. Methods for fragmentation, labeling and immobilization of nucleic acids

Also Published As

Publication number Publication date
JP5079509B2 (ja) 2012-11-21
US8241475B2 (en) 2012-08-14
JP2008511308A (ja) 2008-04-17
DE602005026412D1 (de) 2011-03-31
AU2005278786A1 (en) 2006-03-09
EP1791955A2 (de) 2007-06-06
CA2614580A1 (en) 2006-03-09
WO2006025074A2 (en) 2006-03-09
WO2006025074A3 (en) 2006-06-15
EP1791955B1 (de) 2011-02-16
US20090107841A1 (en) 2009-04-30

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