DE60321449D1 - Verfahren zur zweidimensionalen konformationsabhängigen trennung nichtzirkulärer nukleinsäuren - Google Patents

Verfahren zur zweidimensionalen konformationsabhängigen trennung nichtzirkulärer nukleinsäuren

Info

Publication number
DE60321449D1
DE60321449D1 DE60321449T DE60321449T DE60321449D1 DE 60321449 D1 DE60321449 D1 DE 60321449D1 DE 60321449 T DE60321449 T DE 60321449T DE 60321449 T DE60321449 T DE 60321449T DE 60321449 D1 DE60321449 D1 DE 60321449D1
Authority
DE
Germany
Prior art keywords
electrophoresis
nucleic acids
conformation
linear nucleic
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60321449T
Other languages
English (en)
Inventor
Jon Johannes Jonsson
Hans Guttormur Thormar
Gudmundur H Gunnarsson
Bjarki Gudmundsson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lifeind ehf
Original Assignee
Lifeind ehf
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lifeind ehf filed Critical Lifeind ehf
Application granted granted Critical
Publication of DE60321449D1 publication Critical patent/DE60321449D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE60321449T 2002-02-25 2003-02-25 Verfahren zur zweidimensionalen konformationsabhängigen trennung nichtzirkulärer nukleinsäuren Expired - Lifetime DE60321449D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IS6281 2002-02-25
US36010702P 2002-02-28 2002-02-28
PCT/IS2003/000011 WO2003070943A1 (en) 2002-02-25 2003-02-25 Methods for two-dimensional conformation-dependent separation of non-circular nucleic acids

Publications (1)

Publication Number Publication Date
DE60321449D1 true DE60321449D1 (de) 2008-07-17

Family

ID=27758882

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60321449T Expired - Lifetime DE60321449D1 (de) 2002-02-25 2003-02-25 Verfahren zur zweidimensionalen konformationsabhängigen trennung nichtzirkulärer nukleinsäuren

Country Status (10)

Country Link
US (1) US7306912B2 (de)
EP (1) EP1476549B1 (de)
JP (1) JP4441267B2 (de)
AT (1) ATE397658T1 (de)
AU (1) AU2003206126A1 (de)
CA (1) CA2477345C (de)
DE (1) DE60321449D1 (de)
DK (1) DK1476549T3 (de)
ES (1) ES2309297T3 (de)
WO (1) WO2003070943A1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2614580A1 (en) 2004-09-02 2006-03-09 Lifeind Ehf. Two-dimensional strandness- and length-dependent separation of nucleic acid fragments
WO2011049097A1 (ja) * 2009-10-21 2011-04-28 旭硝子株式会社 インプリント用モールド、その製造方法、インプリント装置およびインプリント方法
GB201102385D0 (en) * 2011-02-10 2011-03-30 Biocule Scotland Ltd Two-dimensional gel electrophoresis apparatus and method
JP6102711B2 (ja) * 2013-12-10 2017-03-29 株式会社島津製作所 電気泳動分離方法
US20190309283A1 (en) * 2016-07-19 2019-10-10 Biodynamics Laboratory, Inc. Method for preparing long-chain single-stranded dna
CN108546735A (zh) * 2018-04-12 2018-09-18 大连理工大学 一种基于牛磺酸的快速制备核酸电泳缓冲液的预制试粉及其使用方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL9100132A (nl) * 1991-01-25 1992-08-17 Ingeny Bv Werkwijze voor het detecteren van dna sequentievariatie.
US5874212A (en) * 1993-05-13 1999-02-23 Thomas Jefferson University Detection of single base mutations and other variations in double stranded DNA by conformation-sensitive gel electrophoresis
US5641626A (en) 1993-10-29 1997-06-24 Fmc Corporation Highly derivatized agarose conformational nucleic acid separation
AU2803397A (en) 1996-04-17 1997-11-07 Ramot University Authority For Applied Research And Industrial Development Ltd. Method and kit for detecting genomic instability

Also Published As

Publication number Publication date
ATE397658T1 (de) 2008-06-15
US20060057575A1 (en) 2006-03-16
JP2005518202A (ja) 2005-06-23
CA2477345C (en) 2017-05-02
EP1476549A1 (de) 2004-11-17
ES2309297T3 (es) 2008-12-16
WO2003070943A1 (en) 2003-08-28
US7306912B2 (en) 2007-12-11
EP1476549B1 (de) 2008-06-04
DK1476549T3 (da) 2009-06-08
JP4441267B2 (ja) 2010-03-31
CA2477345A1 (en) 2003-08-28
AU2003206126A1 (en) 2003-09-09

Similar Documents

Publication Publication Date Title
ATE428931T1 (de) Verfahren zur durchführung von vollbluttests
DK1159453T3 (da) Fremgangsmåde til direkte nukleinsyresekventering
DE60041355D1 (de) Verfahren zur detektion von erkrankungen
ATE515573T1 (de) Verfahren zum nachweis der grösse von nukleinsäuren
WO2003066906A3 (en) Diagnostic microarray and method of use thereof
ATE283473T1 (de) Verfahren zur isolierung von zellen
DE60208278D1 (de) System und Verfahren zum Testen von Nukleinsäuremolekülen
CN105754995A (zh) 构建待测基因组的dna测序文库的方法及其应用
DE602004003842D1 (de) Verfahren und automatisiertes Microfluidsystem zum Nachweis von Proteinen in biologischen Proben
ATE553216T1 (de) Sonden, bibliotheken und kits zur analyse von nukleinsäuregemischen und verfahren zu deren konstruktion
DE60314898D1 (de) Zusammensetzungen und verfahren zur trennung von zellen
ATE466283T1 (de) Zentrifugalvorrichtung und verfahren zur durchführung von bindungsversuchen
DE60303020D1 (de) Verfahren zur gewinnung von nukleinsäuren mit hilfe chaotroper-und ammoniumreagentien
DE602005026412D1 (de) Zweidimensionale strangform- und längenabhängige trennung von nukleinsäurefragmenten
DE60321449D1 (de) Verfahren zur zweidimensionalen konformationsabhängigen trennung nichtzirkulärer nukleinsäuren
DE60323579D1 (de) Methode zur Separierung von Nukleinsäuren und dafür verwendbare Reagenz
Wu et al. Microfluidic chip for stacking, separation and extraction of multiple DNA fragments
DE60226125D1 (de) Verfahren zur quantifizierung von glykiertem protein
ATE402198T1 (de) Verfahren zur immobilisierung biologischer moleküle an feste oberflächen
DE50204680D1 (de) Verfahren zur detektion von nukleinsäuremolekülen
BRPI0406265B8 (pt) método para análise por eletroforese capilar, método para pesquisa e tipagem de proteínas monoclonais, método de preparação de anticorpos modificados sobrecarregados negativamente e kit para detecção e tipagem de proteínas monoclonais
DE602004015932D1 (de) Verfahren zur bildung eines protein-mikroarraysystems
WO2000040757A3 (en) Method of identifying nucleic acids
DE602004011228D1 (de) Verfahren und zusammensetzungen zur bestimmung glycierter proteine
WO2002083929A3 (en) System and method for quantitating transcription factors

Legal Events

Date Code Title Description
8364 No opposition during term of opposition