AR005664A1 - Un metodo para impartir tolerancia frente a un aminoacido analogo al triptofano a una celula de planta de maiz, un metodo para seleccionar celulas de planta de maiz transformadas y un metodo para alterar el contenido de triptofano en una planta de maiz - Google Patents

Un metodo para impartir tolerancia frente a un aminoacido analogo al triptofano a una celula de planta de maiz, un metodo para seleccionar celulas de planta de maiz transformadas y un metodo para alterar el contenido de triptofano en una planta de maiz

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Publication number
AR005664A1
AR005664A1 ARP970100213A ARP970100213A AR005664A1 AR 005664 A1 AR005664 A1 AR 005664A1 AR P970100213 A ARP970100213 A AR P970100213A AR P970100213 A ARP970100213 A AR P970100213A AR 005664 A1 AR005664 A1 AR 005664A1
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Argentina
Prior art keywords
corn plant
tryptophan
amino acid
alter
plant cells
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ARP970100213A
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English (en)
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Dekalab Genetics Corp
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Publication date
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Publication of AR005664A1 publication Critical patent/AR005664A1/es

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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
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    • C12N15/8207Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
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    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
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    • C12N15/09Recombinant DNA-technology
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    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254Tryptophan or lysine
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    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0006Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)

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  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente invención proporciona un método para impartir tolerancia a un aminoácido análogo al triptofano en células de plantas, por medio deintroducir y expresar un segmento aislado de ADN que codifica una antranilato sintetasa en las célulasd e la planta. Se proveen asimismo plantas transgénicastransformadas con un segmento de ADN aislado que codifica una antranilato sintetasa, así como semillas y progenie derivada de esas plantas. Asimismoproporciona una secuencia de ADNc de unasu bunidad alfa y una subunidad beta de una antranilato sintetasa de maíz.
ARP970100213A 1996-01-19 1997-01-20 Un metodo para impartir tolerancia frente a un aminoacido analogo al triptofano a una celula de planta de maiz, un metodo para seleccionar celulas de planta de maiz transformadas y un metodo para alterar el contenido de triptofano en una planta de maiz AR005664A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/604,789 US6118047A (en) 1993-08-25 1996-01-19 Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction

Publications (1)

Publication Number Publication Date
AR005664A1 true AR005664A1 (es) 1999-07-14

Family

ID=24421060

Family Applications (4)

Application Number Title Priority Date Filing Date
ARP970100213A AR005664A1 (es) 1996-01-19 1997-01-20 Un metodo para impartir tolerancia frente a un aminoacido analogo al triptofano a una celula de planta de maiz, un metodo para seleccionar celulas de planta de maiz transformadas y un metodo para alterar el contenido de triptofano en una planta de maiz
ARP980101552A AR011462A2 (es) 1996-01-19 1998-04-06 UN METODO PARA IMPARTIR TOLERANCIA FRENTE A UN AMINOÁCIDO ANÁLOGO DEL TRIPTOFANO, A UNA CÉLULA DE PLANTA DICOTILEDoNEA, UN MÉTODO PARA ALTERAR EL CONTENIDO DE TRIPTOFANO EN UNA PLANTA DICOTILEDoNEA Y UN MÉTODO PARA SELECCIONAR CÉLULAS DE PLANTAS DICOTILEDoNEAS.
ARP070100466A AR059316A2 (es) 1996-01-19 2007-02-05 Planta y semilla transformada con antralinato sintesa resistente a la inhibicion por l-triptofano
ARP070100465A AR059315A2 (es) 1996-01-19 2007-02-05 Planta y semilla transformada

Family Applications After (3)

Application Number Title Priority Date Filing Date
ARP980101552A AR011462A2 (es) 1996-01-19 1998-04-06 UN METODO PARA IMPARTIR TOLERANCIA FRENTE A UN AMINOÁCIDO ANÁLOGO DEL TRIPTOFANO, A UNA CÉLULA DE PLANTA DICOTILEDoNEA, UN MÉTODO PARA ALTERAR EL CONTENIDO DE TRIPTOFANO EN UNA PLANTA DICOTILEDoNEA Y UN MÉTODO PARA SELECCIONAR CÉLULAS DE PLANTAS DICOTILEDoNEAS.
ARP070100466A AR059316A2 (es) 1996-01-19 2007-02-05 Planta y semilla transformada con antralinato sintesa resistente a la inhibicion por l-triptofano
ARP070100465A AR059315A2 (es) 1996-01-19 2007-02-05 Planta y semilla transformada

Country Status (11)

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US (5) US6118047A (es)
EP (1) EP0879292A1 (es)
JP (1) JP2000503541A (es)
AR (4) AR005664A1 (es)
AU (1) AU724987B2 (es)
BR (1) BR9707055A (es)
CA (1) CA2243268A1 (es)
HU (1) HUP9900387A3 (es)
IL (1) IL125070A0 (es)
WO (1) WO1997026366A1 (es)
ZA (1) ZA97399B (es)

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AR059316A2 (es) 2008-03-26
BR9707055A (pt) 1999-07-20
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AR011462A2 (es) 2000-08-16
US6118047A (en) 2000-09-12
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US20030167514A1 (en) 2003-09-04
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HUP9900387A2 (hu) 1999-05-28
AR059315A2 (es) 2008-03-26
IL125070A0 (en) 1999-01-26
AU1706697A (en) 1997-08-11
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