WO2020230886A1 - 細胞培養用足場材料により形成された樹脂膜、細胞培養用担体及び細胞培養用容器 - Google Patents
細胞培養用足場材料により形成された樹脂膜、細胞培養用担体及び細胞培養用容器 Download PDFInfo
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- 108010052768 tyrosyl-isoleucyl-glycyl-seryl-arginine Proteins 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 229910001928 zirconium oxide Inorganic materials 0.000 description 1
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- C08F20/00—Homopolymers and copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride, ester, amide, imide or nitrile thereof
- C08F20/02—Monocarboxylic acids having less than ten carbon atoms, Derivatives thereof
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- C08F16/00—Homopolymers and copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by an alcohol, ether, aldehydo, ketonic, acetal or ketal radical
- C08F16/02—Homopolymers and copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by an alcohol, ether, aldehydo, ketonic, acetal or ketal radical by an alcohol radical
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- C08F261/00—Macromolecular compounds obtained by polymerising monomers on to polymers of oxygen-containing monomers as defined in group C08F16/00
- C08F261/02—Macromolecular compounds obtained by polymerising monomers on to polymers of oxygen-containing monomers as defined in group C08F16/00 on to polymers of unsaturated alcohols
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- C09D151/00—Coating compositions based on graft polymers in which the grafted component is obtained by reactions only involving carbon-to-carbon unsaturated bonds; Coating compositions based on derivatives of such polymers
- C09D151/003—Coating compositions based on graft polymers in which the grafted component is obtained by reactions only involving carbon-to-carbon unsaturated bonds; Coating compositions based on derivatives of such polymers grafted on to macromolecular compounds obtained by reactions only involving unsaturated carbon-to-carbon bonds
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- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
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- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
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- C07K5/10—Tetrapeptides
- C07K5/1019—Tetrapeptides with the first amino acid being basic
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- C08J2351/00—Characterised by the use of graft polymers in which the grafted component is obtained by reactions only involving carbon-to-carbon unsaturated bonds; Derivatives of such polymers
- C08J2351/06—Characterised by the use of graft polymers in which the grafted component is obtained by reactions only involving carbon-to-carbon unsaturated bonds; Derivatives of such polymers grafted on to homopolymers or copolymers of aliphatic hydrocarbons containing only one carbon-to-carbon double bond
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- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/30—Synthetic polymers
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- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
Definitions
- the present invention relates to a resin film formed of a scaffold material for cell culture.
- the present invention also relates to a cell culture carrier and a cell culture container provided with the above resin film.
- hPSC human pluripotent stem cells
- hESC regenerative medicine human embryonic stem cells
- hiPSC human induced pluripotent stem cells
- Patent Document 1 describes a molded product made of a polyvinyl acetal compound or a molded product made of the polyvinyl acetal compound and a water-soluble polysaccharide, and the degree of acetalization of the polyvinyl acetal compound is 20 to 60 mol%.
- a carrier for cell culture is disclosed.
- Patent Document 2 discloses a composition containing the first fibrous polymer scaffolding material, wherein the fibers of the first fibrous polymer scaffolding material are aligned. It is described that the fibers constituting the first fibrous polymer scaffold material are composed of an aliphatic polyester such as polyglycolic acid or polylactic acid.
- Patent Document 3 describes a cell culture method for maintaining the undifferentiated state of pluripotent stem cells, which is pluripotent on an incubator having a surface coated with a polyrotaxan block copolymer. A method including the step of culturing stem cells is disclosed.
- the cell colonization after seeding can be enhanced.
- an adhesive protein such as laminin or vitronectin or a mouse sarcoma-derived matrigel
- the cell colonization after seeding is extremely high.
- natural polymer materials are expensive, have large variations between lots because they are naturally derived substances, and have safety concerns due to animal-derived components.
- scaffolding materials using synthetic resins have better operability, lower cost, less variation between lots, and excellent safety than scaffolding materials using natural polymer materials. ..
- the synthetic resin swells excessively because the synthetic resin having high hydrophilicity is used.
- cell clumps may exfoliate during culturing.
- the scaffolding material using the synthetic resin has low colonization after seeding of the cells, and the cells may not proliferate sufficiently.
- An object of the present invention is to provide a resin membrane formed of a cell culture scaffold material, a cell culture carrier, and a cell culture container, which are excellent in colonization after seeding of cells and can increase the cell growth rate. There is.
- the resin film formed of the cell culture scaffold material according to the present invention has a phase-separated structure in which the cell culture scaffold material contains a synthetic resin and the resin film contains at least a first phase and a second phase.
- the ratio of the surface area of one of the first phase and the second phase to the entire surface is 0.01 or more and 0.95 or less.
- the ratio of the peripheral length to the area of the second phase (peripheral length / area) is 0.001 (1 / nm) or more and 0.40 (1 / nm). ) It is as follows.
- the phase-separated structure is a sea-island structure, the first phase is a sea part, and the second phase is an island part.
- the number of the second phase, which is the island portion is 1 piece / ⁇ m 2 or more and 5000 pieces / ⁇ m 2 or less.
- the phase-separated structure is composed of an intramolecular phase-separated structure of the synthetic resin.
- the dispersion term component of the surface free energy is 25.0 mJ / m 2 or more and 50.0 mJ / m 2 or less
- the polar term component is 1.0 mJ / m. 2 or more and 20.0 mJ / m 2 or less.
- the synthetic resin has a cationic functional group, and the content of the cationic functional group contained in the structural unit of the synthetic resin is 0. It is 2 mol% or more and 50 mol% or less.
- the second phase has a peptide portion.
- the peptide portion has a cell-adherent amino acid sequence.
- the water swelling ratio is 50% or less.
- the storage elastic modulus at 100 ° C. is 1.0 ⁇ 10 4 Pa or more and 1.0 ⁇ 10 8 Pa or less
- the storage elastic modulus at 25 ° C. the ratio of the storage modulus at 100 ° C. ((storage modulus at 25 ° C.) / (storage modulus at 100 ° C.)) is, 1.0 ⁇ 10 1 or more and 1.0 ⁇ 10 5 or less.
- the cell culture scaffold material is substantially free of animal-derived materials.
- the synthetic resin comprises a vinyl polymer.
- the synthetic resin comprises at least a polyvinyl alcohol derivative or a poly (meth) acrylic acid ester.
- the carrier for cell culture according to the present invention includes a carrier and a resin film formed according to the present invention, and the resin film is arranged on the surface of the carrier.
- the cell culture container according to the present invention includes a container body and a resin film configured according to the present invention, and the resin film is arranged on the surface of the container body.
- a resin membrane formed of a cell culture scaffold material, a cell culture carrier, and a cell culture container which are excellent in colonization after seeding of cells and can increase the cell growth rate. be able to.
- the present invention relates to a resin film formed of a scaffold material for cell culture.
- the scaffold material for cell culture contains a synthetic resin.
- the resin film of the present invention has a phase-separated structure including at least a first phase and a second phase.
- the ratio of the surface area of one of the first phase and the second phase to the entire surface is 0.01 or more and 0.95 or less.
- the resin film of the present invention has the above-mentioned structure, it has excellent fixability after seeding of cells and can increase the proliferation rate of cells.
- Scaffolding materials for cell culture using conventional natural polymer materials can improve the colonization of cells after seeding, but they are expensive, and because they are naturally derived substances, there is a large variation between lots, and animals. There are safety concerns due to the ingredients of origin.
- the synthetic resin swells excessively or has a low affinity with cells, so that the cell mass may exfoliate during culturing. Therefore, conventional scaffolding materials using synthetic resins have low colonization after seeding of cells, and cells may not proliferate sufficiently.
- the present inventors have focused on the phase-separated structure of the resin film formed of the cell culture scaffold material, and the ratio of the surface area of one of the first phase and the second phase to the entire surface is specific to the above. It has been found that the phase-separated structure having a range can enhance the affinity with cells, thereby enhancing the adhesiveness after seeding, and thus the proliferation rate of cells. The reason for this is not clear, but when having such a phase-separated structure, the energy distribution proceeds smoothly, and the positions and ratios of the first phase and the second phase having different affinities and intensities are determined. Since it can be adjusted, it is considered that the affinity can be enhanced regardless of the type of cell, and the accumulation and adsorption effect of the cell or cell surface protein can be realized.
- the adhesiveness to the cells after seeding can be enhanced, and the proliferation rate of the cells can be enhanced.
- the synthetic resin can be used as described above, the operability is good, the cost is low, the variation between lots is small, and the variation between lots is small as compared with the scaffolding material using the natural polymer material. It has excellent safety.
- a synthetic resin having a peptide portion may be used as the synthetic resin. Details of the synthetic resin having a peptide portion will be described later.
- the ratio of the surface area (surface area integration rate) of one of the first phase and the second phase to the entire surface is 0.01 or more, preferably 0.10 or more, 0.95 or less, and more. It is preferably 0.90 or less.
- the surface integration rate is within the above range, the adhesiveness to the cells after seeding can be further enhanced, and the cell proliferation rate can be further enhanced.
- examples of the phase-separated structure include microphase-separated structures such as a sea-island structure, a cylinder structure, a gyroid structure, and a lamellar structure.
- the first phase can be the sea part and the second phase can be the island part.
- the phase having the largest surface area can be the first phase, and the phase having the second largest surface area can be the second phase.
- the sea-island structure is preferable as the phase-separated structure. In this way, by having a continuous phase and a discontinuous phase, it is possible to enhance the affinity with the cells and further enhance the adhesiveness with the cells after seeding, thereby further increasing the proliferation rate of the cells. Can be enhanced.
- the surface integration ratio of the second phase with respect to the entire surface is 0.01 or more, preferably 0.1 or more, more preferably 0.2 or more, and 0. It is 95 or less, preferably 0.9 or less, and more preferably 0.8 or less.
- the surface integration rate is within the above range, the adhesiveness to the cells after seeding can be further enhanced, and the cell proliferation rate can be further enhanced.
- the ratio of the peripheral length to the area of the second phase is preferably 0.001 (1 / nm) or more, more preferably 0.0015 (1 / nm) or more, still more preferably 0.008. It is (1 / nm) or more.
- the ratio of the peripheral length to the area of the second phase (peripheral length / area) is preferably 0.40 (1 / nm) or less, more preferably 0.20 (1 / nm) or less, still more preferably 0.08. It is (1 / nm) or less, particularly preferably 0.013 (1 / nm) or less.
- the ratio of the peripheral length to the area of the second phase is preferably 0.001 (1 / nm) or more, more preferably 0.0015 (). It is 1 / nm) or more, preferably 0.08 (1 / nm) or less, and more preferably 0.013 (1 / nm) or less.
- the ratio (peripheral length / area) is within the above range, the adhesiveness to the cells after seeding can be further enhanced, and the cell proliferation rate can be further enhanced.
- the ratio of the peripheral length to the area of the second phase is preferably 0.008 (1 / nm) or more, more preferably 0.013 (1 /). nm) or more, preferably 0.40 (1 / nm) or less, more preferably 0.20 (1 / nm) or less, still more preferably 0.10 (1 / nm) or less.
- the ratio (peripheral length / area) is within the above range, the adhesiveness to the cells after seeding can be further enhanced, and the cell proliferation rate can be further enhanced.
- the number of the second phase is preferably 1 piece / ⁇ m 2 or more, more preferably 2 pieces / ⁇ m 2 or more, further preferably 10 pieces / ⁇ m 2 or more, preferably 5000 pieces / ⁇ m 2 or less. It is more preferably 1000 pieces / ⁇ m 2 or less, further preferably 500 pieces / ⁇ m 2 or less, and particularly preferably 300 pieces / ⁇ m 2 or less.
- the adhesiveness to the cells after seeding can be further enhanced, and the cell proliferation rate can be further enhanced.
- the average diameter of the second phase of the island is preferably 20 nm or more, more preferably 30 nm or more, still more preferably 50 nm or more, particularly preferably 80 nm or more, preferably 3.5 ⁇ m or less, more preferably 3.0 ⁇ m. Below, it is more preferably 1.5 ⁇ m or less.
- the average diameter of the second phase is within the above range, the adhesiveness to the cells after seeding can be further enhanced, and the cell proliferation rate can be further enhanced.
- the average diameter of the second phase is preferably 50 nm or more, more preferably 100 nm or more, still more preferably 120 nm or more, and particularly preferably 200 nm or more. Is 1 ⁇ m or less, more preferably 300 nm or less, still more preferably 250 nm or less.
- the average diameter of the second phase is within the above range, the adhesiveness to the cells after seeding can be further enhanced, and the cell proliferation rate can be further enhanced.
- the average diameter of the second phase is preferably 10 nm or more, more preferably 20 nm or more, still more preferably 40 nm or more, preferably 1 ⁇ m or less, more preferably 300 nm. Below, it is more preferably 100 nm or less.
- the average diameter of the second phase is within the above range, the adhesiveness to the cells after seeding can be further enhanced, and the cell proliferation rate can be further enhanced.
- phase-separated structure and the parameters indicating the phase-separated structure as described above can be confirmed by, for example, an atomic force microscope (AFM), a transmission electron microscope (TEM), a scanning electron microscope (SEM), or the like. can do.
- AFM atomic force microscope
- TEM transmission electron microscope
- SEM scanning electron microscope
- the ratio of the surface area of one of the first phase and the second phase to the entire surface (surface integration rate), the ratio of the peripheral length to the area of the second phase (peripheral length / area),
- the number of the second phases, which are islands, and the average diameter size thereof can be obtained from the above-mentioned microscopic observation image using image analysis software such as ImageJ.
- the ratio of the surface area of one of the first phase and the second phase to the entire surface is one of the first phase and the second phase within the observation region (30 ⁇ m ⁇ 30 ⁇ m). It is obtained by dividing the surface area occupied by the phase by the area of the observation area.
- the ratio of the peripheral length to the area of the second phase is obtained by dividing the total peripheral length of the second phase by the total area of the second phase within the observation area (30 ⁇ m ⁇ 30 ⁇ m). It is required by doing.
- the number of second phases that are islands can be obtained by dividing the number of second phases in the observation region (30 ⁇ m ⁇ 30 ⁇ m) by the area of the observation region. .. Further, the average diameter of the second phase, which is an island portion, is obtained as the average diameter of a circle having the same area.
- phase-separated structure as described above can be obtained by, for example, blending, copolymerizing, graft-copolymerizing at least two different kinds of polymers, or using a synthetic resin having a peptide portion. It can be obtained by forming a phase-separated structure between or within the polymers of. Above all, from the viewpoint of further enhancing the adhesiveness of cells, it is preferable that the phase separation structure is formed by an intramolecular phase separation structure. That is, the synthetic resin is preferably a copolymer of at least two different polymers or a synthetic resin having a peptide portion, and is a graft copolymer or a synthetic resin having a peptide portion. Is more preferable.
- phase-separated structure as described above is obtained by copolymerizing two or more kinds of polymers (monomers) having a solubility parameter (SP value) of 0.1 or more, preferably 0.5 or more, more preferably 1 or more. Is preferable. In this case, the sea-island structure can be formed more easily.
- SP value solubility parameter
- the SP value is a measure of the intermolecular force acting between a solvent and a solute, and is a measure of the affinity between substances.
- the SP value can be determined based on Hidebrand's theory of regular solutions.
- the unit of the SP value is (cal / cm 3 ) 0.5 .
- the Fedors method is described in the Journal of the Japan Adhesive Society, Vol. 22, 1986, p. 566.
- phase separation parameter indicating the phase separation structure such as the surface integration rate is adjusted by, for example, controlling the compounding ratio of the two types of polymers, the structure of the polymer, or controlling the content of the peptide portion. Can be done.
- first phase and another phase different from the second phase there may be a first phase and another phase different from the second phase.
- the other phase may be one phase or a plurality of phases.
- Such a phase can be obtained, for example, by copolymerizing another polymer (monomer) having a different SP value by grafting or the like.
- the two phases occupying the surface of the resin film are designated as the first phase and the second phase.
- the dispersion term component of the surface free energy in the resin film formed of the scaffold material for cell culture is preferably 25.0 mJ / m 2 or more and 50.0 mJ / m 2 or less. Is.
- the hydrophilicity of the scaffold material for cell culture can be appropriately adjusted, and the synergistic effect with the phase-separated structure can further enhance the interfacial adhesiveness with the cells after seeding, and the cell proliferation rate. Can be further enhanced.
- the dispersion term component is more preferably 30.0 mJ / m 2 or more, further preferably 35.0 mJ / m 2 or more, more preferably 47.0 mJ / m 2 or less, still more preferably 45.5 mJ / m 2 or less. is there.
- the polar term component of the surface free energy in the resin film formed of the scaffold material for cell culture is preferably 1.0 mJ / m 2 or more and 20.0 mJ / m 2 or less. Is. In this case, the adhesiveness to the cells after seeding can be further enhanced, and the cell proliferation rate can be further enhanced.
- the polar term component is more preferably 2.0 mJ / m 2 or more, more preferably 3.0 mJ / m 2 or more, more preferably 10.0 mJ / m 2 or less, more preferably 5.0mJ / m 2 or less is there.
- the dispersion term component ⁇ d of the surface free energy and the dipole component ⁇ p which is a polar term component, are calculated using the theoretical formula of Kaelble-Uy.
- the Kaelble-Uy theoretical formula is a theoretical formula based on the assumption that the total surface free energy ⁇ is the sum of the dispersion term component ⁇ d and the dipole component ⁇ p , as shown by the following formula (1). ..
- the surface free energy of the liquid is ⁇ l (mJ / m 2 )
- the surface free energy of the solid is ⁇ s (mJ / m 2 )
- the contact angle is ⁇ (°).
- the liquid surface free energy gamma l of liquid is known using two, measuring the respective contact angle ⁇ with respect to the resin film formed by scaffolding material for cell culture, coalition gamma s d and gamma s p
- the dispersion term component ⁇ d and the dipole component ⁇ p of the surface free energy of the resin film formed from the cell culture scaffold material can be obtained.
- the contact angle ⁇ is measured as follows using a contact angle meter (for example, “DMo-701” manufactured by Kyowa Interface Science Co., Ltd.).
- 1 ⁇ L of pure water or diiodomethane is added dropwise to the surface of the resin film formed of the scaffold material for cell culture.
- the angle formed by the pure water 30 seconds after the dropping and the resin film is defined as the contact angle ⁇ with respect to the pure water.
- the angle formed by the diiodomethane 30 seconds after the dropping and the resin film is defined as the contact angle ⁇ with respect to the diiodomethane.
- the dispersion term component of the surface free energy is described. ⁇ d can be reduced. Further, by increasing the content of hydrophilic functional groups in the synthetic resin or increasing the content of butyl groups, the dipole component ⁇ p of the surface free energy can be reduced.
- the storage elastic modulus at 100 ° C. is preferably 0.6 ⁇ 10 4 Pa or more, more preferably 0.8 ⁇ 10 4 Pa or more, still more preferable. Is 1.0 ⁇ 10 4 Pa or more, preferably 1.0 ⁇ 10 8 Pa or less, more preferably 0.8 ⁇ 10 8 Pa or less, and further preferably 1.0 ⁇ 10 7 Pa or less.
- the resin film formed of the scaffold material for cell culture of the present invention has a ratio of the storage elastic modulus at 25 ° C. to the storage elastic modulus at 100 ° C. ((storage elastic modulus at 25 ° C.) / (storage elastic modulus at 100 ° C.). rate)) is preferably 1.0 ⁇ 10 1 or more, more preferably 5.0 ⁇ 10 1 or more, more preferably 8.0 ⁇ 10 2 or more, preferably 1.0 ⁇ 10 5 or less, more preferably 0.75 ⁇ 10 5 or less, more preferably 0.5 ⁇ 10 5 or less.
- the storage elastic modulus at 25 ° C. and 100 ° C. is, for example, measured by a dynamic viscoelasticity measuring device (manufactured by IT Measurement Control Co., Ltd., DVA-200) under tensile conditions at a frequency of 10 Hz and a temperature range of ⁇ 150 ° C. to 150 ° C. Is measured at a heating rate of 5 ° C./min. From the obtained graph of tensile storage elastic modulus, the storage elastic modulus at 25 ° C. and 100 ° C. is obtained, and the 25 ° C. storage elastic modulus / 100 ° C. storage elastic modulus is calculated.
- a dynamic viscoelasticity measuring device manufactured by IT Measurement Control Co., Ltd., DVA-200
- the storage elastic moduli at 25 ° C. and 100 ° C. can be increased, for example, by increasing the degree of cross-linking in the synthetic resin, stretching the synthetic resin, and the like. Further, the storage elastic moduli at 25 ° C. and 100 ° C. can be lowered by lowering the number average molecular weight of the synthetic resin, lowering the glass transition temperature, and the like.
- the resin film formed from the scaffold material for cell culture of the present invention has a water swelling ratio of preferably 50% or less, more preferably 40% or less. In this case, the colonization of the cells after seeding can be further enhanced.
- the lower limit of the water swelling ratio is not particularly limited, but may be, for example, 0.5%.
- the water swelling ratio can be reduced, for example, by increasing the hydrophobic functional groups of the synthetic resin, lowering the number average molecular weight, and the like.
- the scaffold material for cell culture contains a synthetic resin (hereinafter, may be referred to as synthetic resin X).
- the main chain of the synthetic resin X is preferably a carbon chain.
- a "structural unit” means a repeating unit of a monomer constituting a synthetic resin.
- the synthetic resin has a graft chain, it contains a repeating unit of the monomers constituting the graft chain.
- the synthetic resin X When the synthetic resin X does not have a peptide portion, the synthetic resin X preferably has a cationic functional group.
- the synthetic resin X having a peptide portion may or may not have a cationic functional group in a structural portion other than the peptide portion.
- the cationic functional group include substituents having a structure such as an amino group, an imino group, and an amide group.
- conjugated amine functional groups such as hydroxyamino group, urea group, guanidine, biguanide, piperazine, piperidine, pyrrolidine, 1,4-diazabicyclo [2.2.2] octane, hexamethylenetetraamine, Morpholine, pyridine, pyridazine, pyrimidine, pyrazine, pyrrole, azatropylidene, pyridone, imidazole, benzoimidazole, benzotriazole, pyrazole, oxazole, imidazoline, triazole, thiazole, thiazine, tetrazole, indol, isoindole, purine, quinoline, isoquinoline, quinazoline , Kinoxalin, Synnoline, Pteridine, Carbazole, Acrydin, Adenine, Guanine, Citocin, Timine, Ulacyl,
- the content of the cationic functional group contained in the structural unit of the synthetic resin X is preferably 0.2 mol% or more, preferably 2 mol% or more, more preferably 3 mol% or more, 50 mol%. Hereinafter, it is preferably 10 mol% or less, more preferably 7 mol% or less.
- the content of the cationic functional group can be measured by, for example, 1 H-NMR (nuclear magnetic resonance spectrum).
- the synthetic resin X preferably contains a vinyl polymer, and more preferably a vinyl polymer.
- the vinyl polymer is a polymer of a compound having a vinyl group or a vinylidene group.
- the synthetic resin X is a vinyl polymer, it is possible to more easily suppress the swelling of the scaffold material for cell culture in water.
- the vinyl polymer include polyvinyl alcohol derivatives, poly (meth) acrylic acid esters, polyvinylpyrrolidone, polystyrene, ethylene-vinyl acetate copolymers and the like.
- the vinyl polymer is preferably a polyvinyl alcohol derivative or a poly (meth) acrylic acid ester from the viewpoint of easily enhancing the adhesiveness with cells.
- the scaffold material for cell culture preferably contains a synthetic resin X having a polyvinyl acetal skeleton.
- the synthetic resin X having a polyvinyl acetal skeleton is preferably a copolymer of a structural unit of the polyvinyl acetal resin and a vinyl compound and / or a vinylidene compound.
- the vinyl compound or vinylidene compound may be a vinyl polymer which is a polymer thereof.
- the vinyl compound, vinylidene compound, and vinyl polymer copolymerized with the polyvinyl acetal resin may be generically referred to as "vinyl compound A".
- the copolymer may be a block copolymer of a polyvinyl acetal resin and vinyl compound A, or may be a graft copolymer obtained by grafting vinyl compound A on a polyvinyl acetal resin.
- the copolymer is preferably a graft copolymer. In this case, the phase-separated structure can be formed more easily.
- vinyl compound and vinylidene compound examples include ethylene, allylamine, vinylpyrrolidone, maleic anhydride, maleimide, itaconic acid, (meth) acrylic acid, vinylamine, and (meth) acrylic acid ester. Only one kind of these vinyl compounds may be used, or two or more kinds may be used in combination. Therefore, it may be a vinyl polymer in which these vinyl compounds are copolymerized.
- the difference in SP value between the polyvinyl acetal resin and the vinyl compound A is preferably 0.5 or more. In this case, the phase-separated structure can be formed more easily.
- the difference in SP value between the polyvinyl acetal resin and the vinyl compound A is more preferably 1.0 or more.
- the upper limit of the difference between the SP values is not particularly limited, but may be 10.0, for example.
- the first phase of the above copolymer is a polyvinyl acetal resin and the second phase is a vinyl compound A. It is preferable that the first phase is formed by the polyvinyl acetal resin portion of the copolymer and the second phase is formed by the vinyl compound A portion. In this case, it is preferable that the first phase of the polyvinyl acetal resin is the sea part and the second phase of the vinyl compound A is the island part.
- the first phase of the vinyl compound A may be the sea part
- the second phase of the polyvinyl acetal resin may be the island part.
- the content (molar / mol) of the vinyl compound A in the copolymer is preferably 0.015 or more, more preferably 0.3 or more, preferably 0.95 or less, and more preferably 0.90 or less. More preferably, it is 0.70 or less.
- the content is above the lower limit, the phase-separated structure can be formed more easily.
- the cell proliferation rate can be further increased.
- the polyvinyl acetal resin has an acetal group, an acetyl group, and a hydroxyl group in the side chain.
- the method for synthesizing a polyvinyl acetal resin includes at least a step of acetalizing polyvinyl alcohol with an aldehyde.
- the aldehyde used for acetalizing polyvinyl alcohol to obtain a polyvinyl acetal resin is not particularly limited.
- Examples of the aldehyde include aldehydes having 1 to 10 carbon atoms.
- the aldehyde may have a chain aliphatic group, a cyclic aliphatic group or an aromatic group.
- the aldehyde may be a chain aldehyde or a cyclic aldehyde.
- aldehydes examples include formaldehyde, acetaldehyde, propionaldehyde, butylaldehyde, pentanal, hexanal, heptanal, octanal, nonanal, decanal, achlorine, benzaldehyde, cinnamaldehyde, perylaldehyde, formylpyridine, formylimidazole, formylpyrrole, formylpiperidin, formyl.
- Triazole formyltetrazole, formylindole, formylisoindole, formylpurine, formylbenzoimidazole, formylbenzotriazole, formylquinoline, formylisoquinolin, formylquinoxalin, formylcinnoline, formylpteridine, formylfuran, formyloxolane, formyloxane, Examples thereof include formylthiophene, formylthiolan, formyltian, formyladenine, formylguanine, formylcitosine, formyltimine, formyluracil and the like. Only one of these aldehydes may be used, or two or more of these aldehydes may be used in combination.
- the aldehyde is preferably formaldehyde, acetaldehyde, propionaldehyde, butyraldehyde, or pentanal, and more preferably butyraldehyde. Therefore, the polyvinyl acetal skeleton is preferably a polyvinyl butyral skeleton.
- the polyvinyl acetal resin is preferably a polyvinyl butyral resin.
- the polyvinyl acetal resin preferably has a Bronsted basic group or a Bronsted acidic group, and more preferably has a Bronsted basic group. That is, it is preferable that a part of the polyvinyl acetal resin is modified with a Bronsted basic group or a Bronsted acidic group, and more preferably a part of the polyvinyl acetal resin is modified with a Bronsted basic group.
- Bronsted basic group is a general term for functional groups that can receive hydrogen ion H + from other substances.
- the blended basic group include amine-based basic groups such as a substituent having an imine structure, a substituent having an imide structure, a substituent having an amine structure, and a substituent having an amide structure.
- the blended basic group is not particularly limited, but is, for example, a conjugated amine-based functional group such as a hydroxyamino group, a urea group, guanidine, or biguanide, piperazine, piperidine, pyrrolidine, 1,4-diazabicyclo [2.2.2].
- Examples of the blended acidic group include a carboxyl group, a sulfonic acid group, a maleic acid group, a sulfinic acid group, a sulfenic acid group, a phosphoric acid group, a phosphonic acid group, and salts thereof.
- the Bronsted acidic group is preferably a carboxyl group.
- the polyvinyl acetal resin preferably has a structural unit having an imine structure, a structural unit having an imide structure, a structural unit having an amine structure, or a structural unit having an amide structure. In this case, it may have only one kind of these structural units, or may have two or more kinds.
- the polyvinyl acetal resin may have a structural unit having an imine structure.
- the polyvinyl acetal resin preferably has an imine structure in the side chain.
- the polyvinyl acetal resin may have a structural unit having an imide structure.
- the polyvinyl acetal resin preferably has an imino group in the side chain.
- the imino group may be directly bonded to the carbon atom constituting the main chain of the polyvinyl acetal resin, or may be bonded to the main chain via a linking group such as an alkylene group.
- the polyvinyl acetal resin may have a structural unit having an amine structure.
- the amine group in the above amine structure may be a primary amine group, a secondary amine group, a tertiary amine group, or a quaternary amine group. Good.
- the structural unit having an amine structure may be a structural unit having an amide structure.
- the polyvinyl acetal resin preferably has an amine structure or an amide structure in the side chain.
- the amine structure or the amide structure may be directly bonded to the carbon atom constituting the main chain of the polyvinyl acetal resin, or may be bonded to the main chain via a linking group such as an alkylene group.
- the content of structural units having an imine structure, the content of structural units having an imide structure, the content of structural units having an amine structure, and the content of structural units having an amide structure are 1 H-NMR (nuclear magnetism). It can be measured by the resonance spectrum).
- the vinyl compound A is preferably a (meth) acrylic acid ester or a poly (meth) acrylic acid ester resin.
- the synthetic resin X is a copolymer in which a (meth) acrylic acid ester or a poly (meth) acrylic acid ester resin, which is a polymer thereof, is graft-copolymerized with a polyvinyl acetal resin.
- the poly (meth) acrylic acid ester resin can be obtained by polymerizing the (meth) acrylic acid ester or by polymerizing the (meth) acrylic acid ester with the above-mentioned other monomers.
- (meth) acrylic acid ester examples include (meth) acrylic acid alkyl ester, (meth) acrylic acid cyclic alkyl ester, (meth) acrylic acid aryl ester, (meth) acrylamide, (meth) acrylic acid polyethylene glycol, and ( Meta) Phosphorylcholine acrylate and the like can be mentioned.
- Examples of the (meth) acrylic acid alkyl ester include methyl (meth) acrylate, ethyl (meth) acrylate, n-propyl (meth) acrylate, isopropyl (meth) acrylate, n-butyl (meth) acrylate, and isobutyl (meth) acrylate.
- t-butyl (meth) acrylate n-octyl (meth) acrylate, isooctyl (meth) acrylate, 2-ethylhexyl (meth) acrylate, nonyl (meth) acrylate, isononyl (meth) acrylate, decyl (meth) acrylate, isodecyl ( Examples thereof include meth) acrylate, lauryl (meth) acrylate, stearyl (meth) acrylate, and isotetradecyl (meth) acrylate.
- the (meth) acrylic acid alkyl ester may be substituted with a substituent such as an alkoxy group having 1 to 3 carbon atoms and a tetrahydrofurfuryl group.
- a substituent such as an alkoxy group having 1 to 3 carbon atoms and a tetrahydrofurfuryl group.
- examples of such (meth) acrylic acid alkyl esters include methoxyethyl acrylate and tetrahydrofurfuryl acrylate.
- Examples of the (meth) acrylic acid cyclic alkyl ester include cyclohexyl (meth) acrylate, isobornyl (meth) acrylate and the like.
- Examples of the (meth) acrylic acid aryl ester include phenyl (meth) acrylate, benzyl (meth) acrylate and the like.
- Examples of (meth) acrylamides include (meth) acrylamide, N-isopropyl (meth) acrylamide, N-tert-butyl (meth) acrylamide, N, N'-dimethyl (meth) acrylamide, and (3- (meth) acrylamide propyl).
- Trimethylammonium chloride 4- (meth) acryloylmorpholine, 3- (meth) acryloyl-2-oxazolidinone, N- [3- (dimethylamino) propyl] (meth) acrylamide, N- (2-hydroxyethyl) (meth) ) Acrylamide, N-methylol (meth) acrylamide, 6- (meth) acrylamide hexane acid and the like.
- polyethylene glycols (meth) acrylate examples include methoxy-polyethylene glycol (meth) acrylate, ethoxy-polyethylene glycol (meth) acrylate, hydroxy-polyethylene glycol (meth) acrylate, methoxy-diethylene glycol (meth) acrylate, and ethoxy-.
- examples thereof include diethylene glycol (meth) acrylate, hydroxy-diethylene glycol (meth) acrylate, methoxy-triethylene glycol (meth) acrylate, ethoxy-triethylene glycol (meth) acrylate, and hydroxy-triethylene glycol (meth) acrylate.
- Examples of phosphorylcholine (meth) acrylate include 2- (meth) acryloyloxyethyl phosphorylcholine.
- a vinyl compound is preferably used as another monomer copolymerized with the (meth) acrylic acid ester.
- the vinyl compound include ethylene, allylamine, vinylpyrrolidone, vinylimidazole, maleic anhydride, maleimide, itaconic acid, (meth) acrylic acid, vinylamine, and (meth) acrylic acid ester. Only one kind of vinyl compound may be used, or two or more kinds may be used in combination.
- (meth) acrylic means “acrylic” or “methacryl”
- (meth) acrylate means “acrylate” or “methacrylate”.
- the synthetic resin X may be a copolymer of a resin having a poly (meth) acrylic acid ester skeleton and another vinyl compound as long as the phase-separated structure of the present invention can be formed.
- ethylene, allylamine, vinylpyrrolidone, maleic anhydride, maleimide, itaconic acid, (meth) acrylic acid, vinylamine, or another (meth) acrylic acid ester having a different SP value shall be used. Can be done.
- the scaffold material for cell culture preferably contains a synthetic resin X having a peptide portion.
- the synthetic resin X having a peptide portion can be obtained by reacting the synthetic resin X with a linker and a peptide.
- the synthetic resin X having a peptide portion is preferably a peptide-containing polyvinyl acetal resin having a polyvinyl acetal resin portion, a linker portion, and a peptide portion, and has a polyvinyl butyral resin portion, a linker portion, and a peptide portion.
- a peptide-containing polyvinyl butyral resin is more preferable.
- the synthetic resin X having a peptide portion only one kind may be used, or two or more kinds may be used in combination.
- the peptide portion is preferably composed of 3 or more amino acids, more preferably composed of 4 or more amino acids, further preferably composed of 5 or more amino acids, and 10 or less. It is preferably composed of amino acids, and more preferably composed of 6 or less amino acids.
- the number of amino acids constituting the peptide portion is not less than the above lower limit and not more than the above upper limit, the adhesiveness to the cells after seeding can be further enhanced, and the cell proliferation rate can be further enhanced.
- the peptide portion preferably has a cell-adhesive amino acid sequence.
- the cell adhesion amino acid sequence refers to an amino acid sequence whose cell adhesion activity has been confirmed by the phage display method, the Sepharose beads method, or the plate coating method.
- the phage display method for example, the method described in "The Journal of Cell Biology, Volume 130, Number 5, September 1995 1189-1196" can be used.
- the Sepharose beads method for example, the method described in "Protein Nucleic Acid Enzyme Vol.45 No.15 (2000) 2477” can be used.
- the plate coating method for example, the method described in "Protein Nucleic Acid Enzyme Vol. 45 No. 15 (2000) 2477” can be used.
- cell-adhesive amino acid sequence examples include an RGD sequence (Arg-Gly-Asp), a YIGSR sequence (Tyr-Ile-Gly-Ser-Arg), and a PDSGR sequence (Pro-Asp-Ser-Gly-Arg).
- HAV sequence His-Ala-Val
- ADT sequence Alignment sequence
- QAV sequence Gln-Ala-Val
- LDV sequence Leu-Asp-Val
- IDS sequence Ile-Asp-Ser
- REDV sequence Arg-Glu-Asp-Val
- IDAPS sequence Ile-Asp-Ala-Pro-Ser
- KQAGDV sequence Lys-Gln-Ala-Gly-Asp-Val
- TDE sequence Thr-Asp- Glu
- the peptide portion may have only one type of the cell-adhesive amino acid sequence, or may have two or more types.
- the cell-adherent amino acid sequence preferably has at least one of the cell-adherent amino acid sequences described above, and more preferably has at least an RGD sequence, a YIGSR sequence, or a PDSGR sequence, and the following formula (1) It is more preferable to have at least the RGD sequence represented by).
- the adhesiveness to the cells after seeding can be further enhanced, and the cell proliferation rate can be further enhanced.
- X represents Gly, Ala, Val, Ser, Thr, Phe, Met, Pro, or Asn.
- the peptide portion may be linear or may have a cyclic peptide skeleton.
- the cyclic peptide skeleton is a cyclic skeleton composed of a plurality of amino acids. From the viewpoint of more effectively exerting the effects of the present invention, the cyclic peptide skeleton is preferably composed of 4 or more amino acids, preferably 5 or more amino acids, and 10 or less. It is preferably composed of the amino acids of.
- the content of the peptide portion is preferably 0.1 mol% or more, more preferably 1 mol% or more, still more preferably 5 mol% or more, and particularly preferably 10 mol% or more. is there.
- the content of the peptide portion is preferably 60 mol% or less, more preferably 50 mol% or less, still more preferably 35 mol% or less, and particularly preferably 25 mol% or less.
- the content rate (mol%) of the peptide portion is the amount of substance of the peptide portion with respect to the total amount of substances of each structural unit constituting the synthetic resin X having the peptide portion.
- the content of the peptide portion can be measured by FT-IR or LC-MS.
- the second phase has a peptide portion, and the peptide portion More preferably has the cell-adhesive amino acid sequence. It is preferable that the second phase is formed by the peptide portion portion of the synthetic resin X having the peptide portion. In this case, it is preferable that the second phase having the peptide portion is the island portion. However, the first phase may have a peptide portion, and the second phase having a peptide portion may be a sea portion.
- the synthetic resin X having a peptide portion it is preferable that the synthetic resin X portion and the peptide portion are bonded via a linker. That is, the synthetic resin X having a peptide portion is preferably a synthetic resin X having a peptide portion and a linker portion. Only one type of the linker may be used, or two or more types may be used in combination.
- the linker is preferably a compound having a functional group capable of condensing with the carboxyl group or amino group of the peptide.
- the functional group capable of condensing with the carboxyl group or amino group of the peptide include a carboxyl group, a thiol group and an amino group.
- the linker is preferably a compound having a carboxyl group.
- the vinyl compound A described above can also be used.
- Examples of the linker having a carboxyl group include (meth) acrylic acid and acrylamide containing a carboxyl group.
- a carboxylic acid (carboxylic acid monomer) having a polymerizable unsaturated group as the linker having a carboxyl group, the carboxylic acid monomer can be polymerized by graft polymerization when the linker and the synthetic resin X are reacted. Therefore, the number of carboxyl groups that can be reacted with the peptide can be increased.
- the scaffold material for cell culture contains the above synthetic resin X.
- the content of the synthetic resin X in 100% by weight of the scaffold material for cell culture is preferably 90% by weight or more, more preferably 90% by weight or more. It is 95% by weight or more, more preferably 97.5% by weight or more, particularly preferably 99% by weight or more, and most preferably 100% by weight (total amount). Therefore, it is most preferable that the scaffold material for cell culture is the synthetic resin X.
- the content of the synthetic resin X is at least the above lower limit, the effect of the present invention can be exhibited even more effectively.
- the cell culture scaffold material may contain components other than the synthetic resin X.
- the components other than the synthetic resin X include polyolefin resins, polyether resins, polyvinyl alcohol resins, polyesters, epoxy resins, polyamide resins, polyimide resins, polyurethane resins, polycarbonate resins, polysaccharides, celluloses, polypeptides, synthetic peptides and the like. Can be mentioned.
- the content of the component in 100% by weight of the scaffold material for cell culture is preferably 10% by weight or less, more preferably 5% by weight or less, still more preferably 2.5% by weight or less, and particularly preferably 1% by weight or less. , Most preferably 0% by weight (not contained). Therefore, it is most preferable that the scaffold material for cell culture does not contain any component other than the synthetic resin X.
- the scaffold material for cell culture does not substantially contain animal-derived raw materials.
- the animal-derived raw materials in the cell culture scaffold material are 3% by weight or less.
- the animal-derived raw material in the cell culture scaffold material is preferably 1% by weight or less, and more preferably 0% by weight. That is, it is more preferable that the scaffold material for cell culture does not contain any animal-derived raw materials.
- the above-mentioned scaffold material for cell culture is used for culturing cells.
- the above-mentioned scaffold material for cell culture is used as a scaffold for the cells when culturing the cells. Therefore, the resin film formed from the cell culture scaffold material of the present invention is used for culturing cells, and is also used as a scaffold for the cells when culturing the cells.
- Examples of the above cells include animal cells such as humans, mice, rats, pigs, cows and monkeys.
- Examples of the cells include somatic cells and the like, and examples thereof include stem cells, progenitor cells and mature cells.
- the somatic cells may be cancer cells.
- Examples of the mature cells include nerve cells, cardiomyocytes, retinal cells, hepatocytes and the like.
- stem cells examples include mesenchymal stem cells (MSCs), iPS cells, ES cells, Muse cells, embryonic cancer cells, embryonic reproductive stem cells, and mGS cells.
- the resin film of the present invention is formed of a scaffold material for cell culture.
- the resin film is formed by using a scaffold material for cell culture.
- the resin film is preferably a film-like scaffold material for cell culture.
- the resin film is preferably a film-like material for a scaffold material for cell culture.
- the present specification also provides particles, fibers, porous bodies, or films containing the above-mentioned scaffold material for cell culture.
- the shape of the scaffold material for cell culture is not particularly limited, and may be particles, fibers, porous bodies, or films.
- the particles, fibers, porous body, or film may contain components other than the scaffold material for cell culture.
- the film containing the scaffold material for cell culture is preferably used for plane culture (two-dimensional culture) of cells.
- particles, fibers, or porous bodies containing the above-mentioned scaffold material for cell culture are preferably used for three-dimensional culture of cells.
- the present invention also relates to a carrier for cell culture in which the resin film is arranged on the surface of the carrier.
- the carrier for cell culture of the present invention can be obtained, for example, by arranging the resin film on the surface of the carrier by coating or the like.
- the shape of the carrier may be particles, fibers, porous material, or film. That is, the cell culture carrier of the present invention may be in the form of particles, fibers, porous bodies, or films.
- the cell culture carrier of the present invention may contain components other than the carrier and the resin film.
- FIG. 1 is a cross-sectional view schematically showing a cell culture container according to an embodiment of the present invention.
- the cell culture container 1 includes a container body 2 and a resin film 3 formed of a scaffold material for cell culture.
- the resin film 3 is arranged on the surface 2a of the container body 2.
- the resin film 3 is arranged on the bottom surface of the container body 2.
- the container body may include a first container body and a second container body such as a cover glass on the bottom surface of the first container body.
- the first container body and the second container body may be separable.
- a resin film formed of the cell culture scaffold material may be arranged on the surface of the second container body.
- a conventionally known container body can be used as the container body.
- the shape and size of the container body are not particularly limited.
- Examples of the container body include a cell culture plate having one or more wells (holes), a cell culture flask, and the like.
- the number of wells in the plate is not particularly limited.
- the number of wells is not particularly limited, and examples thereof include 2, 4, 6, 12, 24, 48, 96, and 384.
- the shape of the well is not particularly limited, and examples thereof include a perfect circle, an ellipse, a triangle, a square, a rectangle, and a pentagon.
- the shape of the bottom surface of the well is not particularly limited, and examples thereof include a flat bottom, a round bottom, and unevenness.
- the material of the container body is not particularly limited, and examples thereof include resin, metal, and inorganic materials.
- the resin include polystyrene, polyethylene, polypropylene, polycarbonate, polyester, polyisoprene, cycloolefin polymer, polyimide, polyamide, polyamideimide, (meth) acrylic resin, epoxy resin, silicone and the like.
- the metal include stainless steel, copper, iron, nickel, aluminum, titanium, gold, silver, platinum and the like.
- the inorganic material include silicon oxide (glass), aluminum oxide, titanium oxide, zirconium oxide, iron oxide, silicon nitride and the like.
- the following synthetic resins were synthesized as raw materials for scaffolding materials for cell culture.
- Example 1 To a reactor equipped with a stirrer, 300 parts by weight of polyvinyl alcohol having 2700 mL of ion-exchanged water, an average degree of polymerization of 1700 and a saponification degree of 98 mol% was added and dissolved by heating while stirring to obtain a solution. To the obtained solution, 35% by weight hydrochloric acid was added as a catalyst so that the hydrochloric acid concentration was 0.2% by weight. Then, the temperature was adjusted to 15 ° C., and 22 parts by weight of n-butyraldehyde was added with stirring. Next, 148 parts by weight of n-butyraldehyde was added to precipitate a white particulate polyvinyl butyral resin.
- the obtained polyvinyl butyral resin was dissolved in tetrahydrofuran so as to be a 1% by weight solution, and 5 parts by weight of Irgacure184 as an initiator, 2 parts by weight of N-vinylpyrrolidone (SP value: 11.7) and A synthetic resin was obtained by adding 8 parts by weight of n-lauryl methacrylate (SP value: 8.2) and performing graft polymerization.
- the obtained synthetic resin had an acetalization degree (butyralization degree) of 69 mol%, a hydroxyl group content of 27.5 mol%, an acetylation degree of 2.0 mol%, a vinylpyrrolidone group content of 0.3 mol%, and n-.
- the content of the lauryl methacrylate part was 1.2 mol%.
- Example 2 to 11 and Comparative Example 1 A synthetic resin was obtained in the same manner as in Example 1 except that the weight ratios of the polyvinyl butyral resin, N-vinylpyrrolidone, and n-lauryl methacrylate were changed.
- Table 1 shows the acetalization degree (butyralization degree), the amount of hydroxyl groups, the degree of acetylation, the content of vinylpyrrolidone groups, and the content of the n-lauryl methacrylate portion of the synthetic resins obtained in Examples 2 to 11 and Comparative Example 1. , Table 2 and Table 4.
- Example 12 To a reactor equipped with a stirrer, 300 parts by weight of polyvinyl alcohol having 2700 mL of ion-exchanged water, an average degree of polymerization of 1700 and a saponification degree of 99 mol% was added and dissolved by heating while stirring to obtain a solution. To the obtained solution, 35% by weight hydrochloric acid was added as a catalyst so that the hydrochloric acid concentration was 0.2% by weight. Then, the temperature was adjusted to 15 ° C., and 22 parts by weight of n-butyraldehyde was added with stirring.
- n-butyraldehyde was added to precipitate a white particulate polyvinyl acetal resin (polyvinyl butyral resin).
- 35% by weight hydrochloric acid was added so that the hydrochloric acid concentration became 1.8% by weight, and then the mixture was heated to 50 ° C. and maintained at 50 ° C. for 2 hours.
- polyvinyl butyral resin was washed with water and dried to obtain a polyvinyl acetal resin (polyvinyl butyral resin, average degree of polymerization 1700, degree of acetalization (degree of butyralization) 70 mol%, amount of hydroxyl groups). 27 mol%, degree of acetylation 3 mol%) was obtained.
- linker 99 parts by weight of the obtained polyvinyl acetal resin and 1 part by weight of acrylic acid (linker) are dissolved in 300 parts by weight of THF and reacted in the presence of a photoradical polymerization initiator for 20 minutes under ultraviolet irradiation to obtain a polyvinyl acetal resin.
- a linker was introduced by graft-copolymerizing with acrylic acid.
- 1 part by weight of the polyvinyl acetal resin into which the linker was introduced was dissolved in 19 parts by weight of butanol.
- 150 ⁇ L of the obtained solution was discharged onto the surface of a ⁇ 22 mm cover glass (“22 Maru No.
- Peptide formation A linear peptide having an amino acid sequence of Gly-Arg-Gly-Asp-Ser (five amino acid residues, described as GRGDS in the table) was prepared. 10 parts by weight of this peptide and 1 part by weight of 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride (condensant) were added to the peptide in phosphate buffered saline containing neither calcium nor magnesium. Was added to a final concentration of 1 mM to prepare a peptide-containing solution.
- this peptide-containing liquid 1 part by weight of this peptide-containing liquid was added to a spin-coated resin film (polyvinyl acetal resin forming a linker) and reacted to dehydrate and condense the carboxyl group of the linker and the amino group of Gly of the peptide.
- a peptide-containing polyvinyl acetal resin having a polyvinyl acetal resin portion, a linker portion, and a peptide portion was produced.
- the obtained peptide-containing polyvinyl acetal resin has an acetalization degree (butyralization degree) of 69 mol%, a hydroxyl group amount of 27 mol%, an acetylation degree of 3 mol%, a carboxyl group content of 0.1 mol%, and a peptide portion.
- the rate was 1.0 mol%.
- Example 13 Examples except that 85 parts by weight of polyvinyl acetal resin and 15 parts by weight of acrylic acid (linker) were used in the introduction of the linker, and the amount of the peptide added was changed to 15 parts by weight in the formation of the peptide.
- a peptide-containing polyvinyl acetal resin was prepared in the same manner as in 12.
- Example 14 Examples except that 70 parts by weight of polyvinyl acetal resin and 30 parts by weight of acrylic acid (linker) were used in the introduction of the linker, and the amount of the peptide added was changed to 30 parts by weight in the formation of the peptide.
- a peptide-containing polyvinyl acetal resin was prepared in the same manner as in 12.
- Example 15 Examples except that 67 parts by weight of polyvinyl acetal resin and 33 parts by weight of acrylic acid (linker) were used in the introduction of the linker, and the amount of the peptide added was changed to 33 parts by weight in the formation of the peptide.
- a peptide-containing polyvinyl acetal resin was prepared in the same manner as in 12.
- Example 16 Examples except that 63 parts by weight of polyvinyl acetal resin and 37 parts by weight of acrylic acid (linker) were used in the introduction of the linker, and the amount of the peptide added was changed to 37 parts by weight in the formation of the peptide.
- a peptide-containing polyvinyl acetal resin was prepared in the same manner as in 12.
- Example 17 Examples except that 30 parts by weight of polyvinyl acetal resin and 70 parts by weight of acrylic acid (linker) were used in the introduction of the linker, and the amount of the peptide added was changed to 70 parts by weight in the formation of the peptide.
- a peptide-containing polyvinyl acetal resin was prepared in the same manner as in 12.
- a container for cell culture was obtained by arranging a laminate of Vitronectin and a cover glass on a polystyrene dish having a diameter of 22 mm.
- Vitronectin is denatured when dried and the adhesive performance is significantly deteriorated, it was immersed in a PBS solution immediately after the preparation of the cell culture container.
- the storage elastic modulus of each scaffold material at 25 ° C. and 100 ° C. is measured by a dynamic viscoelasticity measuring device (manufactured by IT Measurement Control Co., Ltd., DVA-200) under tensile conditions at a frequency of 10 Hz and a temperature range of ⁇ 150 ° C. to 150 ° C. Was measured at a heating rate of 5 ° C./min. From the obtained graph of tensile storage elastic modulus, the storage elastic modulus at 25 ° C. and 100 ° C. was obtained, and the 25 ° C. storage elastic modulus / 100 ° C. storage elastic modulus was calculated.
- a resin solution was obtained by dissolving 1 g of the obtained synthetic resin in 19 g of butanol. 150 ⁇ L of the obtained resin solution is discharged onto a cover glass of ⁇ 22 mm (manufactured by Matsunami Co., Ltd., 22 circle No. 1 is used after removing dust with an air duster), and rotated at 2000 rpm for 20 seconds using a spin coater to make a smooth resin. A film was obtained.
- a container for cell culture was obtained by arranging the obtained resin film together with the cover 26 glass on a polystyrene dish having a diameter of 22 mm.
- a container for cell culture was obtained by arranging the obtained laminate of the peptide-containing polyvinyl acetal resin and the cover glass on a polystyrene dish having a diameter of 22 mm.
- the surface free energy of the resin film obtained in the column of preparation of the cell culture container was measured using a contact angle meter (DMo-701 manufactured by Kyowa Surface Chemical Co., Ltd.). 1 ⁇ L of pure water was dropped onto the resin film, and a droplet image was taken 30 seconds later to obtain a contact angle of pure water. Further, 1 ⁇ L of diiodomethane was dropped onto the resin film, and a droplet image was taken 30 seconds later to obtain a contact angle of diiodomethane. From the obtained contact angle, the dispersion term component ⁇ d (dSFE) of the surface free energy and the dipole component ⁇ p (psFE), which is the polar term component, were calculated using the theoretical formula of Kaelble-Uy.
- the polyvinyl butyral resin portion as the first phase forms a sea portion
- the resin portion having the (meth) acrylic acid ester and the vinyl compound as the second phase The sea-island structure in which (copolymer part of N-vinylpyrrolidone and n-lauryl methacrylate) forms an island part was observed.
- a sea-island structure was observed in which the polyvinyl butyral resin portion as the first phase formed a sea portion and the peptide portion as the second phase formed an island portion.
- Comparative Examples 1 to 4 no phase-separated structure was observed.
- the ratio of the surface area to the entire surface of the second phase (surface integration rate of the phase-separated structure) of the second phase, the second The ratio of the peripheral length of the phase to the area (peripheral length / area), the number of second phases that are islands (number of islands), and the average diameter of the islands (average island size) were determined.
- Cell proliferation rate 1 mL of phosphate buffered saline was added to the obtained cell culture vessel and allowed to stand in an incubator at 37 ° C. for 1 hour, and then the phosphate buffered saline in the culture vessel was removed. Colonies of h-iPS cells 253G1 in a confluent state were added to a 35 mm dish, then 1 mL of 0.5 mM ethylenediamine / phosphate buffer solution was added, and the mixture was allowed to stand at room temperature for 2 minutes.
- cell mass was broken into 50 ⁇ 200 [mu] m to (0.5 ⁇ 10 5 cells) in the culture vessel by pipetting TeSRE8 medium 1 mL.
- Medium TeSR E8 (STEM CELL Co.) 1.7 mL, and, in the presence of a ROCK-Inhibitor (Y27632) 10 ⁇ M, were cultured at 37 ° C. and 5% CO 2 in an incubator. The medium was replaced by removing 1 mL of the medium every 24 hours and adding 1 mL of new TeSR E8.
- the colonized cell mass after 5 days was exfoliated with 1.0 mL of TryPLE Express exfoliant solution, and the number of cells was counted using a cell counter (Nucleocounter NC-3000, manufactured by Chemometec).
- the cell proliferation rate with respect to Reference Example A was determined using the following formula.
- the cell proliferation rate was evaluated according to the following criteria.
- AAA ... Cell growth rate for Reference Example A is 70% or more AA ... Cell growth rate for Reference Example A is 60% or more and less than 70% A ... Cell growth rate for Reference Example A is 50% or more and less than 60% B ... Cell growth rate for Reference Example A is 40% or more and less than 50% C ... Cell growth rate for Reference Example A is 30% or more and less than 40% D ... Cell growth rate for Reference Example A is less than 30%
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Abstract
Description
細胞培養用足場材料は、合成樹脂(以下、合成樹脂Xと記載することがある)を含む。合成樹脂Xの主鎖は、炭素鎖であることが好ましい。なお、本明細書において、「構造単位」とは、合成樹脂を構成するモノマーの繰り返し単位をいう。なお、合成樹脂がグラフト鎖を有する場合は、そのグラフト鎖を構成するモノマーの繰り返し単位を含む。
合成樹脂Xは、ビニル重合体を含むことが好ましく、ビニル重合体であることがより好ましい。なお、ビニル重合体とは、ビニル基又はビニリデン基を有する化合物の重合体である。上記合成樹脂Xがビニル重合体である場合、水中における細胞培養用足場材料の膨潤をより抑制しやすくすることができる。ビニル重合体としては、例えば、ポリビニルアルコール誘導体、ポリ(メタ)アクリル酸エステル、ポリビニルピロリドン、ポリスチレン、エチレン・酢酸ビニル共重合体等が挙げられる。さらに、ビニル重合体としては、細胞との接着性をより高めやすい観点から、ポリビニルアルコール誘導体又はポリ(メタ)アクリル酸エステルであることが好ましい。
細胞培養用足場材料は、ポリビニルアセタール骨格を有する合成樹脂Xを含むことが好ましい。本発明において、ポリビニルアセタール骨格を有する合成樹脂Xは、ポリビニルアセタール樹脂の構造単位とビニル化合物及び/又はビニリデン化合物との共重合体であることが好ましい。ビニル化合物は、ビニル基(H2C=CH-)を有する化合物である。ビニリデン化合物は、ビニリデン基(H2C=CR-)を有する化合物である。ビニル化合物又はビニリデン化合物は、その重合体であるビニル重合体であってもよい。なお、以下の説明では、ポリビニルアセタール樹脂に共重合されるビニル化合物、ビニリデン化合物、及びビニル重合体を総称して「ビニル化合物A」とすることがある。
以下、ポリビニルアセタール樹脂(共重合体のポリビニルアセタール樹脂部分)についてより詳細に説明する。
以下、ビニル化合物Aについてより詳細に説明する。
細胞培養用足場材料は、ペプチド部を有する合成樹脂Xを含むことが好ましい。ペプチド部を有する合成樹脂Xは、合成樹脂Xと、リンカーと、ペプチドとを反応させて得ることができる。ペプチド部を有する合成樹脂Xは、ポリビニルアセタール樹脂部と、リンカー部と、ペプチド部とを有するペプチド含有ポリビニルアセタール樹脂であることが好ましく、ポリビニルブチラール樹脂部と、リンカー部と、ペプチド部とを有するペプチド含有ポリビニルブチラール樹脂であることがより好ましい。ペプチド部を有する合成樹脂Xは、1種のみが用いられてもよく、2種以上が併用されてもよい。
細胞培養用足場材料は、上記合成樹脂Xを含む。本発明の効果を効果的に発揮させる観点及び生産性を高める観点からは、上記細胞培養用足場材料100重量%中、上記合成樹脂Xの含有量は、好ましくは90重量%以上、より好ましくは95重量%以上、更に好ましくは97.5重量%以上、特に好ましくは99重量%以上、最も好ましくは100重量%(全量)である。したがって、上記細胞培養用足場材料は、上記合成樹脂Xであることが最も好ましい。上記合成樹脂Xの含有量が上記下限以上であると、本発明の効果をより一層効果的に発揮させることができる。
上記細胞培養用足場材料は、細胞を培養するために用いられる。上記細胞培養用足場材料は、細胞を培養する際の該細胞の足場として用いられる。したがって、本発明の細胞培養用足場材料により形成された樹脂膜は、細胞を培養するために用いられ、また、細胞を培養する際の該細胞の足場として用いられる。
本発明の樹脂膜は、細胞培養用足場材料により形成される。上記樹脂膜は、細胞培養用足場材料を用いて形成される。上記樹脂膜は、膜状の細胞培養用足場材料であることが好ましい。上記樹脂膜は、細胞培養用足場材料の膜状物であることが好ましい。
本発明は、担体の表面上に上記樹脂膜が配置されている、細胞培養用担体にも関する。本発明の細胞培養用担体は、例えば、担体の表面上に上記樹脂膜をコーティング等によって配置することによって得ることができる。上記担体の形状は、粒子、繊維、多孔体、又はフィルムであってもよい。すなわち、本発明の細胞培養用担体は、粒子、繊維、多孔体、又はフィルムの形状であってもよい。なお、本発明の細胞培養用担体は、上記担体及び上記樹脂膜以外の構成要素を含んでいてもよい。
本発明は、細胞の培養領域の少なくとも一部に上記樹脂膜を備える、細胞培養用容器にも関する。図1は、本発明の一実施形態に係る細胞培養用容器を模式的に示す断面図である。
攪拌装置を備えた反応機に、イオン交換水2700mL、平均重合度1700、鹸化度98モル%のポリビニルアルコールを300重量部投入し、攪拌しながら加熱溶解し、溶液を得た。得られた溶液に、触媒として、塩酸濃度が0.2重量%となるように35重量%塩酸を添加した。次いで、温度を15℃に調整し、攪拌しながらn-ブチルアルデヒド22重量部を添加した。次いで、n-ブチルアルデヒド148重量部を添加し、白色粒子状のポリビニルブチラール樹脂を析出させた。析出してから15分後に、塩酸濃度が1.8重量%となるように35重量%塩酸を添加した後、50℃に加熱し、50℃で2時間保持した。次いで、溶液を冷却し、中和した後、水洗し、乾燥させることにより、ポリビニルアセタール樹脂としてのポリビニルブチラール樹脂(PVB、SP値:9.9)を得た。得られたポリビニルブチラール樹脂90重量部を、1重量%の溶液となるようにテトラヒドロフランに溶解させ、開始剤としてIrgacure184を5重量部、N-ビニルピロリドン(SP値:11.7)2重量部及びn-ラウリルメタクリレート(SP値:8.2)8重量部を添加し、グラフト重合を行うことで、合成樹脂を得た。得られた合成樹脂は、アセタール化度(ブチラール化度)69モル%、水酸基量27.5モル%、アセチル化度2.0モル%、ビニルピロリドン基の含有率0.3モル%、n-ラウリルメタクリレート部の含有率1.2モル%であった。
ポリビニルブチラール樹脂、N-ビニルピロリドン、及びn-ラウリルメタクリレートの重量比率を変更したこと以外は、実施例1と同様にして合成樹脂を得た。実施例2~11及び比較例1で得られた合成樹脂のアセタール化度(ブチラール化度)、水酸基量、アセチル化度、ビニルピロリドン基の含有率、n-ラウリルメタクリレート部の含有率を表1、表2、及び表4に示す。
攪拌装置を備えた反応機に、イオン交換水2700mL、平均重合度1700、鹸化度99モル%のポリビニルアルコールを300重量部投入し、攪拌しながら加熱溶解し、溶液を得た。得られた溶液に、触媒として、塩酸濃度が0.2重量%となるように35重量%塩酸を添加した。次いで、温度を15℃に調整し、攪拌しながらn-ブチルアルデヒド22重量部を添加した。次いで、n-ブチルアルデヒド148重量部を添加し、白色粒子状のポリビニルアセタール樹脂(ポリビニルブチラール樹脂)を析出させた。析出してから15分後に、塩酸濃度が1.8重量%になるように35重量%塩酸を添加した後、50℃に加熱し、50℃で2時間保持した。次いで、溶液を冷却し、中和した後、ポリビニルブチラール樹脂を水洗し、乾燥させて、ポリビニルアセタール樹脂(ポリビニルブチラール樹脂、平均重合度1700、アセタール化度(ブチラール化度)70モル%、水酸基量27モル%、アセチル化度3モル%)を得た。
得られたポリビニルアセタール樹脂99重量部と、アクリル酸(リンカー)1重量部とをTHF300重量部に溶解し、光ラジカル重合開始剤の存在下で、紫外線照射下で20分間反応させ、ポリビニルアセタール樹脂とアクリル酸とをグラフト共重合させることにより、リンカーを導入した。リンカーを導入したポリビニルアセタール樹脂1重量部をブタノール19重量部に溶解させた。得られた溶液150μLを、エアダスターで除塵したφ22mmのカバーガラス(松浪社製「22丸No.1」)の表面上に吐出し、スピンコーターを用いて2000rpm、20秒回転させた後、60℃で60分間加熱して、表面が平滑な樹脂膜を得た。
Gly-Arg-Gly-Asp-Serのアミノ酸配列を有する直鎖状のペプチド(アミノ酸残基数5個、表ではGRGDSと記載)を用意した。このペプチド10重量部と、1-エチル-3-(3-ジメチルアミノプロピル)カルボジイミド塩酸塩(縮合剤)1重量部とを、カルシウム及びマグネシウムの双方を含まないリン酸緩衝生理食塩水に該ペプチドの終濃度が1mMとなるよう添加し、ペプチド含有液を作製した。このペプチド含有液1重量部を、スピンコートした樹脂膜(リンカーを形成したポリビニルアセタール樹脂)に添加し、反応させて、リンカーのカルボキシル基と、ペプチドのGlyのアミノ基とを脱水縮合した。このようにして、ポリビニルアセタール樹脂部と、リンカー部と、ペプチド部とを有するペプチド含有ポリビニルアセタール樹脂を作製した。
リンカーの導入において、85重量部のポリビニルアセタール樹脂と15重量部のアクリル酸(リンカー)とを用いたこと、ペプチドの形成において、ペプチドの添加量を15重量部に変更したこと以外は、実施例12と同様にして、ペプチド含有ポリビニルアセタール樹脂を作製した。
リンカーの導入において、70重量部のポリビニルアセタール樹脂と30重量部のアクリル酸(リンカー)とを用いたこと、ペプチドの形成において、ペプチドの添加量を30重量部に変更したこと以外は、実施例12と同様にして、ペプチド含有ポリビニルアセタール樹脂を作製した。
リンカーの導入において、67重量部のポリビニルアセタール樹脂と33重量部のアクリル酸(リンカー)とを用いたこと、ペプチドの形成において、ペプチドの添加量を33重量部に変更したこと以外は、実施例12と同様にして、ペプチド含有ポリビニルアセタール樹脂を作製した。
リンカーの導入において、63重量部のポリビニルアセタール樹脂と37重量部のアクリル酸(リンカー)とを用いたこと、ペプチドの形成において、ペプチドの添加量を37重量部に変更したこと以外は、実施例12と同様にして、ペプチド含有ポリビニルアセタール樹脂を作製した。
リンカーの導入において、30重量部のポリビニルアセタール樹脂と70重量部のアクリル酸(リンカー)とを用いたこと、ペプチドの形成において、ペプチドの添加量を70重量部に変更したこと以外は、実施例12と同様にして、ペプチド含有ポリビニルアセタール樹脂を作製した。
合成樹脂として、ポリスチレン樹脂をそのまま用いた。
攪拌装置を備えた反応機に、イオン交換水2700mL、平均重合度1700、鹸化度98モル%のポリビニルアルコールを300重量部投入し、攪拌しながら加熱溶解し、溶液を得た。得られた溶液に、触媒として、塩酸濃度が0.2重量%となるように35重量%塩酸を添加した。次いで、温度を15℃に調整し、攪拌しながらn-ブチルアルデヒド22重量部を添加した。次いで、n-ブチルアルデヒド148重量部を添加し、白色粒子状のポリビニルブチラール樹脂を析出させた。析出してから15分後に、塩酸濃度が1.8重量%となるように35重量%塩酸を添加した後、50℃に加熱し、50℃で2時間保持した。次いで、溶液を冷却し、中和した後、水洗し、乾燥させることによりポリビニルブチラール樹脂(SP値:9.9)を得た。すなわち、ビニル化合物が共重合していないポリビニルブチラール樹脂(合成樹脂)を得た。
N-ビニルピロリドン17重量部及びn-ラウリルメタクリレート83重量部を混合し、(メタ)アクリルモノマー溶液を得た。得られた(メタ)アクリルモノマー溶液にIrgacure184(BASF社製)1重量部を溶解させ、PETフィルム上に塗布した。塗布物を25℃にてアイグラフィックス社製、UVコンベア装置「ECS301G1」を用い、365nmの波長の光を積算光量2000mJ/cm2で照射することでポリ(メタ)アクリル酸エステル樹脂溶液を得た。得られたポリ(メタ)アクリル酸エステル樹脂溶液を80℃、3時間真空乾燥させることでポリ(メタ)アクリル酸エステル樹脂としての合成樹脂を得た。
天然物由来の足場材の作製:
リン酸バッファー(PBS)中に5μg/mlに調整したVitronectin(コーニング社製)溶液をφ35mmディッシュに1ml添加した。そこにφ22mmのカバーガラス(松浪社製「22丸No.1」)を浸漬させ、37℃で1時間養生することでVitronectinが表面に平滑に吸着した天然物由来の足場材(表中ではVTNと記載)を得た。
Vitronectinとカバーガラスとの積層体を、φ22mmのポリスチレンディッシュに配置することにより細胞培養用容器を得た。なお、Vitronectinは乾燥すると変性し、接着性能が大きく低下してしまうため、細胞培養用容器の作製後すぐにPBS溶液で浸漬した。
(アセタール化度及びカチオン性基変性度)
実施例及び比較例で得られた合成樹脂のアセタール化度及びカチオン性基変性度は、合成樹脂をDMSO-d6(ジメチルスルホキサイド)に溶解した後、1H-NMR(核磁気共鳴スペクトル)により測定した。
各足場材料の25℃及び100℃の貯蔵弾性率は、動的粘弾性測定装置(アイティー計測制御社製、DVA-200)により引張条件下、周波数10Hz、-150℃から150℃の温度範囲を昇温速度5℃/分にて測定した。得られた引張貯蔵弾性率のグラフから25℃及び100℃における貯蔵弾性率を求め、25℃貯蔵弾性率/100℃貯蔵弾性率を算出した。長さ50mm、幅5~20mm、厚み0.1~1.0mmの測定サンプルを用いて、10Hz、ひずみ0.1%、温度-150℃~150℃、および昇温速度5℃/minの条件で行った。
長さ50mm、幅10mm、厚み0.05mm~0.15mmの各足場材料からなる樹脂膜(測定サンプル)を、25℃の水に24時間浸漬した。浸漬前と後のサンプルの重さを測定し、水膨潤倍率=(浸漬後のサンプル重量-浸漬前のサンプル重量)/(浸漬前のサンプル重量)×100(%)を算出した。
実施例1~11及び比較例1~4では、得られた合成樹脂1gをブタノール19gに溶解させることで、樹脂溶液を得た。得られた樹脂溶液150μLをφ22mmのカバーガラス(松浪社製、22丸No.1をエアダスターで除塵して使用)上に吐出し、スピンコーターを用いて2000rpm、20秒回転させて平滑な樹脂膜を得た。得られた上記樹脂膜をカバー26ガラスごとφ22mmのポリスチレンディッシュに配置することにより細胞培養用容器を得た。実施例12~17では、得られたペプチド含有ポリビニルアセタール樹脂とカバーガラスとの積層体を、φ22mmのポリスチレンディッシュに配置することにより細胞培養用容器を得た。
細胞培養用容器の調製の欄で得られた樹脂膜の表面自由エネルギーについて接触角計(協和界面化学社製、DMo-701)を用いて測定した。樹脂膜上に純水1μLを滴下し、30秒後の液滴像を撮影することで純水の接触角を得た。また、上記樹脂膜上にジヨードメタン1μLを滴下し、30秒後の液滴像を撮影することでジヨードメタンの接触角を得た。得られた接触角から、Kaelble-Uyの理論式を用いて表面自由エネルギーの分散項成分γd(dSFE)及び極性項成分である双極子成分γp(pSFE)を算出した。
細胞培養用容器の調製の欄で得られた樹脂膜を原子間力顕微鏡(AFM、ブルカー社製、品番「Dimension XR」)により観察した。カンチレバーはSCAN ASYST AIRを使用した。その結果、図2に示すように、実施例3の樹脂膜では、第1の相としてのポリビニルブチラール樹脂部が海部を形成し、第2の相としての(メタ)アクリル酸エステル及びビニル化合物を有する樹脂部(N-ビニルピロリドン及びn-ラウリルメタクリレートの共重合体部)が島部を形成する海島構造が観察された。同様に、実施例1~2,4~11においても、第1の相としてのポリビニルブチラール樹脂部が海部を形成し、第2の相としての(メタ)アクリル酸エステル及びビニル化合物を有する樹脂部(N-ビニルピロリドン及びn-ラウリルメタクリレートの共重合体部)が島部を形成する海島構造が観察された。また、実施例12~17においても、第1の相としてのポリビニルブチラール樹脂部が海部を形成し、第2の相としてのペプチド部が島部を形成する海島構造が観察された。他方、比較例1~4では、相分離構造が観察されなかった。
得られた細胞培養用容器にリン酸緩衝生理食塩水1mLを加えて37℃のインキュベーター内で1時間静置後、培養容器内のリン酸緩衝生理食塩水を除いた。35mmディッシュにコンフルエント状態になったh-iPS細胞253G1のコロニーを加え、次に1mLの0.5mMエチレンジアミン/リン酸緩衝溶液を加え、室温で2分静置した。その後、エチレンジアミン/リン酸緩衝溶液を除き、1mLのTeSRE8培地でピペッティングにより50~200μmに砕かれた細胞塊(0.5×105cells)を培養容器に播種した。培地TeSR E8(STEM CELL社製)1.7mL、及び、ROCK-Inhibitor(Y27632)10μMの存在下で、37℃及びCO2濃度5%のインキュベーター内で培養を行った。24時間毎に培地を1mL除き、新たなTeSR E8を1mL加えることで培地交換を行った。5日後における定着細胞塊を、TryPLE Express剥離液 1.0mLを用いて剥離し、セルカウンター(Nucleocounter NC-3000、Chemometec社製)を用いて細胞数をカウントした。
AAA…参考例Aに対する細胞増殖率が、70%以上
AA…参考例Aに対する細胞増殖率が、60%以上、70%未満
A…参考例Aに対する細胞増殖率が、50%以上、60%未満
B…参考例Aに対する細胞増殖率が、40%以上、50%未満
C…参考例Aに対する細胞増殖率が、30%以上、40%未満
D…参考例Aに対する細胞増殖率が、30%未満
2…容器本体
2a…表面
3…樹脂膜
Claims (16)
- 細胞培養用足場材料により形成された樹脂膜であって、
前記細胞培養用足場材料は、合成樹脂を含み、
前記樹脂膜が、少なくとも第1の相及び第2の相を含む、相分離構造を有し、
前記第1の相及び第2の相のうち一方の相の表面全体に対する表面積の比が、0.01以上、0.95以下である、樹脂膜。 - 前記第2の相の面積に対する周囲長の比(周囲長/面積)が、0.001(1/nm)以上、0.40(1/nm)以下である、請求項1に記載の樹脂膜。
- 前記相分離構造が、海島構造であり、
前記第1の相が海部であり、前記第2の相が島部である、請求項1又は2に記載の樹脂膜。 - 前記島部である前記第2の相の個数が、1個/μm2以上、5000個/μm2以下である、請求項3に記載の樹脂膜。
- 前記相分離構造が、前記合成樹脂の分子内における相分離構造により構成されている、請求項1~4のいずれか1項に記載の樹脂膜。
- 表面自由エネルギーの分散項成分が25.0mJ/m2以上、50.0mJ/m2以下、かつ、極性項成分が1.0mJ/m2以上、20.0mJ/m2以下である、請求項1~5のいずれか1項に記載の樹脂膜。
- 前記合成樹脂が、カチオン性官能基を有し、
前記合成樹脂の構造単位中に含まれる前記カチオン性官能基の含有量が、0.2モル%以上、50モル%以下である、請求項1~6のいずれか1項に記載の樹脂膜。 - 前記第2の相が、ペプチド部を有する、請求項1~5のいずれか1項に記載の樹脂膜。
- 前記ペプチド部が、細胞接着性のアミノ酸配列を有する、請求項8に記載の樹脂膜。
- 水膨潤倍率が50%以下である、請求項1~9のいずれか1項に記載の樹脂膜。
- 100℃における貯蔵弾性率が、1.0×104Pa以上、1.0×108Pa以下であり、
25℃における貯蔵弾性率と100℃における貯蔵弾性率との比((25℃における貯蔵弾性率)/(100℃における貯蔵弾性率))が、1.0×101以上、1.0×105以下である、請求項1~10のいずれか1項に記載の樹脂膜。 - 前記細胞培養用足場材料は、動物由来の原料を実質的に含まない、請求項1~11のいずれか1項に記載の樹脂膜。
- 前記合成樹脂がビニル重合体を含む、請求項1~12のいずれか1項に記載の樹脂膜。
- 前記合成樹脂が、少なくともポリビニルアルコール誘導体又はポリ(メタ)アクリル酸エステルを含む、請求項1~13のいずれか1項に記載の樹脂膜。
- 担体と、
請求項1~14のいずれか1項に記載の樹脂膜と、を備え、
前記担体の表面上に、前記樹脂膜が配置されている、細胞培養用担体。 - 容器本体と、
請求項1~14のいずれか1項に記載の樹脂膜と、を備え、
前記容器本体の表面上に、前記樹脂膜が配置されている、細胞培養用容器。
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WO2020230885A1 (ja) | 2020-11-19 |
CN116904079A (zh) | 2023-10-20 |
US20220348858A1 (en) | 2022-11-03 |
EP3971202A1 (en) | 2022-03-23 |
TW202108754A (zh) | 2021-03-01 |
JP2024111315A (ja) | 2024-08-16 |
SG11202110134UA (en) | 2021-12-30 |
CN113166719A (zh) | 2021-07-23 |
EP3971201A1 (en) | 2022-03-23 |
SG11202110132QA (en) | 2021-12-30 |
JPWO2020230884A1 (ja) | 2020-11-19 |
EP3971201A4 (en) | 2023-11-22 |
EP3971200A1 (en) | 2022-03-23 |
AU2020274457A1 (en) | 2021-09-30 |
JP2024033003A (ja) | 2024-03-12 |
EP3971200A4 (en) | 2023-08-02 |
CN115926568A (zh) | 2023-04-07 |
WO2020230884A1 (ja) | 2020-11-19 |
TWI839518B (zh) | 2024-04-21 |
JPWO2020230886A1 (ja) | 2020-11-19 |
US20220227898A1 (en) | 2022-07-21 |
AU2020274933A1 (en) | 2021-09-30 |
KR20220009367A (ko) | 2022-01-24 |
CN113166580B (zh) | 2023-07-07 |
EP3971202A4 (en) | 2023-11-22 |
US20220325221A1 (en) | 2022-10-13 |
TW202104577A (zh) | 2021-02-01 |
JPWO2020230885A1 (ja) | 2020-11-19 |
CN113166201A (zh) | 2021-07-23 |
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