US20080145912A1 - Endoglucanases - Google Patents

Endoglucanases Download PDF

Info

Publication number
US20080145912A1
US20080145912A1 US11/740,076 US74007607A US2008145912A1 US 20080145912 A1 US20080145912 A1 US 20080145912A1 US 74007607 A US74007607 A US 74007607A US 2008145912 A1 US2008145912 A1 US 2008145912A1
Authority
US
United States
Prior art keywords
amino acid
sequence
seq
enzyme
xaa
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US11/740,076
Inventor
Martin Schulein
Torben Henriksen
Lene Nonboe Andersen
Soren Flensted Lassen
Markus Sakari Kauppinen
Lene Lange
Ruby Ilum Nielsen
Shinobu Takagi
Michiko Ihara
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27545143&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=US20080145912(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novozymes AS filed Critical Novozymes AS
Priority to US11/740,076 priority Critical patent/US20080145912A1/en
Publication of US20080145912A1 publication Critical patent/US20080145912A1/en
Priority to US12/567,302 priority patent/US8642730B2/en
Priority to US13/683,165 priority patent/US9023620B2/en
Abandoned legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
    • D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06P—DYEING OR PRINTING TEXTILES; DYEING LEATHER, FURS OR SOLID MACROMOLECULAR SUBSTANCES IN ANY FORM
    • D06P5/00—Other features in dyeing or printing textiles, or dyeing leather, furs, or solid macromolecular substances in any form
    • D06P5/02—After-treatment
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06P—DYEING OR PRINTING TEXTILES; DYEING LEATHER, FURS OR SOLID MACROMOLECULAR SUBSTANCES IN ANY FORM
    • D06P5/00—Other features in dyeing or printing textiles, or dyeing leather, furs, or solid macromolecular substances in any form
    • D06P5/15—Locally discharging the dyes
    • D06P5/158—Locally discharging the dyes with other compounds
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
    • D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
    • D21H21/00—Non-fibrous material added to the pulp, characterised by its function, form or properties; Paper-impregnating or coating material, characterised by its function, form or properties
    • D21H21/06—Paper forming aids
    • D21H21/10—Retention agents or drainage improvers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
    • D21H17/00—Non-fibrous material added to the pulp, characterised by its constitution; Paper-impregnating material characterised by its constitution
    • D21H17/005—Microorganisms or enzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/141—Feedstock
    • Y02P20/145—Feedstock the feedstock being materials of biological origin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Definitions

  • the present invention relates to novel enzyme preparations comprising an enzyme exhibiting endoglucanase activity which performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.
  • Cellulases or cellulytic enzymes are enzymes involved in hydrolysis of cellulose.
  • cellobiohydrolase (1,4-beta-D-glucan cellobiohydrolase, EC 3.2.1.91)
  • endo-beta-1,4-glucanase endo-1,4-beta-D-glucan 4-glucanohydrolase, EC 3.2.1.4
  • beta-glucosidase EC 3.2.1.21
  • Cellulases are synthesized by a large number of microorganisms which include fungi, to actinomycetes, mycobacteria and true bacteria but also by plants. Especially endoglucanases of a wide variety of specificities have been identified.
  • cellulytic enzymes A very important industrial use of cellulytic enzymes is the use for treatment of cellulosic textile or fabric, e.g., as ingredients in detergent compositions or fabric softener compositions, for bio-polishing of new fabric (garment finishing), and for obtaining a “stone-washed” look of cellulose containing fabric, especially denim, and several methods for such treatment have been suggested, e.g., in GB-A-1 368 599, EP-A-0 307 564 and EP-A-0 435 876, WO 91/17243, WO 91/10732, WO 91/17244, PCT/DK95/000108 and PCT/DK95/00132.
  • cellulytic enzymes Another important industrial use of cellulytic enzymes is the use for treatment of paper pulp, e.g., for improving the drainage or for deinking of recycled paper.
  • endoglucanases (EC No. 3.2.1.4) constitute an interesting group of hydrolases for the mentioned industrial uses.
  • Endoglucanases catalyses endo hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (such as carboxy methyl cellulose and hydroxy ethyl cellulose), lichenin, beta-1,4 bonds in mixed beta-1,3 glucans such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts.
  • the authorized name is endo-1,4-beta-D-glucan 4-glucano hydrolase, but the abbreviated term endoglucanase is used in the present specification.
  • Fungal endoglucanases have been described in numerous publications, especially those derived from species as, e.g., Fusarium oxysporum, Trichoderma reesei, Trichoderma longibrachiatum, Aspergillus aculeatus, Neocallimastix patriciarum , and, e.g., from species of the genera Piromyces, Humicola, Myceliophthora, Geotricum, Penicillium, Irpex, Coprinus.
  • fungal endoglucanases have been described by Sheppard, P. O., et al., “The use of conserved cellulase family-specific sequences to clone Cellulase homologue cDNAs from Fusarium oxysporum , Gene, Vol. 15, pp. 163-167 (1994), Saloheimo, A., et al., “A novel, small endoglucanase gene, egl5, from Trichoderma reesei isolated by expression in yeast”, Molecular Microbiology Vol. 13(2), pp. 219-228 (1994); van Arsdell, J. N.
  • Neocallimastix patriciarum A novel polysaccharide hydrolase cDNA (celD) from Neocallimastix patriciarum encoding three multi-functional catalytical domains with high endoglucanase, cellobiohydrolase and xylanase activities”, 3. Gen. Microbiol., Vol. 138, pp. 2397-2403 (1992), Zhou, L. et al., “Intronless celB from the anaerobic fungus Neocallimastix patriciarum encodes a modular family A endoglucanase”, Biochem. J., Vol. 297, pp. 359-364 (1994); Dalb ⁇ ge, H. and Heldt-Hansen. H. P.
  • WO 91/17243 discloses a cellulase preparation consisting of a homogenous endoglucanase component immunoreactive with an antibody raised against a highly purified 43 kDa endoglucanase derived from Humicola insolens , DSM 1800;
  • WO 91/17244 discloses a new (hemi)cellulose degrading enzyme, such as an endoglucanase, a cellobiohydrolase or a beta-glucosidase, which may be derived from fungi other than Trichoderma and Phanerochaete ;
  • WO 93/20193 discloses an endoglucanase derivable from Aspergillus aculeatus ;
  • WO 94/21801 (Genencor Inc.) concerns a cellulase system isolated from Trichoderma longibrachiatum exhibiting endoglucanase activity, WO 94/26880 (G)
  • V. discloses an isolated mixture of cellulose degrading enzymes, which preferable are obtained from Trichoderma Aspergillus or Disporotrichum , comprising endoglucanase, cellobiohydrolase, and xyloglucanase activity; and WO 95/02043 (Novo Nordisk A/S) describes an enzyme with endoglucanase activity derived from Trichoderma harzianum , which can be used for a number of purposes including e.g., degradation or modification of plant cell walls.
  • cellulases may or may not have a cellulose binding domain (a CBD).
  • the CBD enhances the binding of the enzyme to a cellulose containing fiber and increases the efficacy of the catalytic active part of the enzyme.
  • the object of the present invention is to provide novel enzyme preparations having substantial cellulytic activity at acid, neutral or alkaline conditions and improved performance in paper pulp processing, textile treatment, laundry processes or in animal feed; preferably novel cellulases, more preferably well-performing endoglucanases, which are contemplated to be producible or produced by recombinant techniques.
  • the present invention relates to an enzyme preparation consisting essentially of an enzyme having cellulytic activity and comprising a first amino acid sequence consisting of 14 amino acid residues having the following sequence
  • the amino acid is Trp, Tyr or Phe;
  • the amino acid is Trp, Tyr or Phe;
  • the amino acid is Arg, Lys or His;
  • the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala.
  • the invention relates to a method for providing a gene encoding for cellulytic enzymes having the above conserved regions.
  • DNA encoding for said enzymes can be found from a broad range of fungi, belonging to taxonomically very different organisms and inhabiting ecologically very different niches.
  • CBD cellulose binding domain
  • the present invention provides novel cellulases, especially endoglucanases, having improved performance in industrial applications, either in their native form, or homo- or heterologously produced.
  • the present invention relates to novel cellulytic enzyme preparations which are derivable from taxonomically specific phyli, classes, orders, families, genera, and species; e.g., from Basidiomycotous Hymenomycetes, Zygomycota, Chytridiomycota ; or from the classes Discomycetes, Loculoascomycetes, Plectomycetes; Archaeascomycetes, Hemiascomycetes or from the orders Diaportales, Xylariales, Trichosphaeriales, Phyllachorales ; or from the families Nectriaeae, Sordariaceae, Chaetomiaceae, Ceratostomaceae, Lasiosphaeriaceae ; or from the genera Cylindrocarpon, Gliocladium, Volutella, Scytalidium, Acremonium , or from the species Fusarium lycopersici, Fusarium passiflora,
  • the enzyme preparation of the invention is preferably obtainable from the taxonomically specific phyli, classes, orders, families, genera, and species mentioned above which all produce endoglucanases comprising a first peptide consisting of 13 amino acid residues having the following sequence
  • the present invention provides DNA constructs comprising a DNA sequence encoding an enzyme exhibiting endoglucanase activity, which DNA sequence comprises the DNA sequence of SEQ ID NOS: 1, 7, 9, 11, 13, 15, 21, and 25, respectively, or analogues thereof.
  • the present invention also relates to a recombinant expression vector comprising a DNA construct of the invention, to a cell comprising a DNA construct or a recombinant expression vector of the invention; to a method of producing an enzyme, e.g., a recombinant enzyme, of the invention; to a method of providing colour clarification of laundry by using the enzyme of the invention to a laundry composition comprising the enzyme of the invention, to uses of the enzyme of the invention for degradation or modification of plant material, e.g., cell walls, for treatment of fabric, textile or garment, for treatment of paper pulp; and to an enzyme preparation which is enriched in an enzyme of the present invention.
  • an enzyme e.g., a recombinant enzyme
  • FIGS. 1A , 1 B and 1 C show an alignment of the deduced encoded amino acid sequences of Acremonium sp, (I) (SEQ ID NO: 8), Volutella colletotrichoides (SEQ ID NO: 22), Crinipellis scabella (SEQ ID NO: 16), Acremonium sp. (II) (SEQ ID NO: 10), Myceliophthora thermophila (SEQ ID NO: 2), Thielavia terrestris (SEQ ID NO: 12), Macrophomina phaseolina (SEQ ID NO: 14).
  • the Pileup program (Feng and Doolittle, 1987) (GCG package, version 8.0) was used to create the best alignment. Identical residues in at least four sequences (boxed) are indicated around the corresponding amino acids.
  • FIGS. 2A , B and C illustrate the taxonomic classification within the Fungal Kingdom of all the microorganisms disclosed herein as being capable of producing said enzyme preparations and enzymes of the invention.
  • taxonomic classification used herein builds primarily on the system used in the NIH Data Base (Entrez, version spring 1996) available on the internet.
  • FIGS. 3A and 38 show an alignment of the deduced partial amino acid sequences derived from a selection of 26 of the 46 microorganisms described in Example 5 (SEQ ID NOS: 40, 30, 38, 74, 64, 32, 52, 62, 66, 28, 34, 68, 76, 72, 46, 54, 42, 36, 48, 44, 78, 58, 50, 60, and 56).
  • the term “the 20 naturally occurring amino acid residues” denotes the amino acid residues usually found in proteins and conventionally known as alanine (Ala or A), valine (Val or V), leucine (Leu or L), isoleucine (Ile or I), proline (Pro or P), phenylalanine (Phe or F), tryptophan (Trp or W), methionine (Met or M), glycine (Gly or G), serine (Ser or S), threonine (Thr or T), cysteine (Cys or C), tyrosine (Tyr or Y), asparagine (Asn or N), glutamine (Gln or Q), aspartic acid (Asp or D), glutamic acid (Glu or E), lysine (Lys or K), arginine (Arg or R), and histidine (H is or H).
  • novel well-performing endoglucanases comprising conserved amino acid sequence regions, especially a first amino acid sequence consisting of 14 amino acid residues having the following sequence
  • the amino acid is Trp, Tyr or Phe;
  • the amino acid is Trp, Tyr or Phe;
  • the amino acid is Arg, Lys or His;
  • the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala.
  • the enzyme of the invention is of microbial origin, i.e., obtainable from a microorganism such as a fungus.
  • the amino acid residue at position 9 of the first sequence is selected from the group consisting of proline, threonine, valine, alanine, leucine, isoleucine, phenylalanine, glycine, cysteine, asparagine, glutamine, tyrosine, serine, methionine and tryptophan, preferably from the group consisting of proline and threonine.
  • the amino acid residue at position 10 of the first sequence is selected from the group consisting of proline, threonine, valine, alanine, leucine, isoleucine, phenylalanine, glycine, cysteine, asparagine, glutamine, tyrosine, serine, methionine and tryptophan, preferably serine.
  • the amino acid residue at position 12 of the first sequence is selected from the group consisting of proline, threonine, valine, alanine, leucine, isoleucine, phenylalanine, glycine, cysteine, asparagine, glutamine, tyrosine, serine, methionine and tryptophan, preferably from the group consisting of alanine and glycine.
  • the amino acid residue at position 14 of the first sequence is selected from the group consisting of proline, threonine, valine, alanine, leucine, isoleucine, phenylalanine, glycine, cysteine, asparagine, glutamine, tyrosine, serine, methionine, tryptophan, glutamic acid and aspartic acid, preferably from the group consisting of proline, threonine, serine, alanine, glutamic acid and aspartic acid.
  • the amino acid residue at position 4 of the second sequence is selected from the to group consisting of proline, threonine, valine, alanine, leucine, isoleucine, phenylalanine, glycine., cysteine, asparagine, glutamine, tyrosine, serine, methionine, tryptophan, glutamic acid and aspartic acid, more preferably from the group consisting of alanine, glycine, and glutamine.
  • Examples of more preferred embodiments are such wherein, in the first sequence, the amino acid residue at position 3 is tyrosine; or the amino acid residue at position 4 is tryptophan; or the amino acid residue at position 8 is lysine.
  • the enzyme of the invention has a first sequence comprising the amino acid sequence
  • the present invention provides a method for providing a microbial strain comprising a gene encoding such an enzyme which method comprises hybridization, e.g., PCR amplification, under standard conditions with an oligonucleotide derived from any of the conserved regions, illustrated in FIG. 1 .
  • a useful oligonucleotide comprises a nucleotide sequence encoding at least a pentapeptide comprised in a peptide selected from the group consisting of
  • Trp Cys Cys Xaa Cys Tyr (SEQ ID NO. 81) 1 2 3 4 5 6 the amino acid at position 4 being any of the 20 naturally occurring amino acid residues; and c.
  • amino acid at position 3 being any of the 20 naturally occurring amino acid residues; the amino acid at positions 4 and 6, respectively, being Trp, Tyr or Phe; and the amino acid at position 9 being Phe or Met;
  • the useful oligonucleotides also comprise nucleotide sequences complementary to the sequences mentioned.
  • the oligonucleotide corresponds to a PCR primer selected from the PCR primers
  • the present invention provides an enzyme preparation which essentially consists of an enzyme having cellulytic activity and having the conserved regions found by the inventors, i.e., which comprises a peptide consisting of 7 amino acid residues having the following sequence (SEQ ID NOS: 105-107)
  • This enzyme is obtainable from a strain belonging to Basidiomycotous Hymenomycetes (see FIG. 2 ), more preferably to the group consisting of the orders Agaricales, Auriculariales , and Aphyllophorales , even more preferably to the group consisting of the families Exidiaceae, Tricholomataceae, Coprinaceae, Schizophyllaceae, Bjerkanderaceae and Polyporaceae , especially to the group consisting of the genera Exidia, Crinipellis, Fomes, Panaeolus, Trametes, Schizophyllum , and Spongipellis.
  • endoglucanases obtainable from a strain belonging to the group consisting of the species Exidia glandulosa, Crinipellis scabella, Fomes fomentarius , and Spongipellis sp., more specific examples being Exidia glandulosa , CBS 277.96 , Crinipellis scabella , CBS 280.96 , Fomes fomentarius , CBS 276.96, and Spongipellis sp., CBS 283.96.
  • Exidia glandulosa was deposited at Centraalbureau voor Schimmelcultures, Oosterstraat 1, Postbus 273, NL-3740 AG Baarn, the Netherlands, on 12 Mar., 199, under the deposition number CBS 277.96 ; Crinipellis scabella was deposited at Centraalbureau voor Schimmelcultures on 12 Mar., 1996, under the deposition number CBS 280.96 , Fomes fomentarius was deposited at Centraalbureau voor Schimmelcultures on 12 Mar., 1996, under the deposition number CBS 276.96, and Spongipellis sp. was deposited at Centraalbureau voor Schimmelcultures on 12 Mar., 1996, under the deposition number CBS 283.96, all deposited under the Budapest Treaty.
  • the enzyme preparation of the invention is also obtainable from a strain belonging to Chytridiomycota , preferably from a strain belonging to the class of Chytridiomycetes , more preferably belonging to the group consisting of the order Spizellomycetales , even more preferably to the family Spizellomycetaceae , especially belonging to the genus Rhizophlyctis .
  • a specific example is a strain belonging to the species Rhizophlyctis rosea , more specifically to Rhizophlyctis rosea , CBS 282.96.
  • Rhizophlyctis rosea was deposited at Centraalbureau voor Schimmelcultures on 12 Mar. 1996, under the deposition number CBS 282.96, under the Budapest Treaty.
  • the enzyme preparation of the invention is also obtainable from a strain belonging to Zygomycota , preferably belonging to the class Zygomycetes , more preferably to the order Mucorales , even more preferably to the group of families consisting of Mucoraceae and Thamnidiaceae , especially belonging to the group consisting of the genera Rhizomucor, Phycomyces and Chaetostylum .
  • the enzyme preparation of the invention is also obtainable from a strain belonging to the group consisting of Archaeascomycetes, Discomycetes, Hemiascomycetes, Loculoascomycetes , and Plectomycetes , preferably belonging to the group consisting of the orders Pezizales, Rhytismatales, Dothideales , and Eurotiales .
  • the enzyme is obtainable from a strain belonging the group consisting of the families Cucurbitariaceae, Ascobolaceae.
  • Rhytismataceae, and Trichocomaceae preferably belonging the group consisting of the genera Diplodia, Microsphaeropsis, Ulospora, Macrophomina, Ascobolus, Saccobolus, Penicillium , and Thermomyces .
  • Specific examples are enzymes obtainable from a strain belonging the group consisting of the species Diplodia gossypina, Microsphaeropsis sp., Ulospora bilgramii, Aureobasidium sp.
  • Macrophomina phaseolina Ascobolus stictoides, Saccobolus diutellus, Peziza, Pencillium verruculosum, Penicillium chrysogenum , and Thermomyces verrucosus , more specifically Diplodia gossypina , CBS 274.96 , Ulospora bilgramii NKBC 1444 , Macrophomina phaseolina , CBS 281.96 , Saccobolus dilutellus , CBS 275.96 , Penicillium verruculosum , ATCC 62396 , Penicillium chrysogenum , ATCC 9480, and Thermomyces verrucosus , CBS 285.96.
  • Diplodia gossypina was deposited at Centraalbureau voor Schimmelcultures on 12 Mar., 1996, under the deposition number CBS 274.96, Macrophomina phaseolina was deposited at Centraalbureau voor Schimmelcultures on 12 Mar., 1996, under the deposition number CBS 281.96 , Saccobolus dilutellus was deposited at Centraalbureau voor Schimmelcultures on 12 Mar., 1996, under the deposition number CBS 275.96; Thermomyces verrucosus was deposited at Centraalbureau voor Schimmelcultures on 12 Mar., 1996, under the deposition number CBS 285.96; all under the Budapest Treaty.
  • the enzyme is obtainable from a strain belonging to the group consisting of the orders Diaportales, Xylariales, Trichosphaeriales and Phyllachorales , preferably from a strain belonging to the group consisting of the families Xylariaceae, Vaisaceae, and Phyllachoraceae, more preferably belonging to the genera Diaporthe, Colletotrichum, Nigrospora, Xylaria, Nodulisporum and Poronia .
  • Diaporthe syngenesia Colletotrichum lagenarium, Xylaria hypoxylon, Nigrospora sp., Nodulisporum sp., and Poronia to punctata , more specifically Diaporthe syngenesia .
  • CBS 278.96 Colletotrichum lagenarium , ATCC 52609, Nigrospora sp., CBS 272.96, Xylaria hypoxylon , CBS 284.96.
  • Diaporthe syngenesia was deposited at Centraalbureau voor Schimmelcultures on 12 Mar., 1996, under the deposition number CBS 278.96
  • Nigrospora sp. was deposited at Centraalbureau voor Schimmelcultures on 12 Mar., 1996, under the deposition number CBS 272.96
  • Xylaria hypoxylon was deposited at Centraalbureau voor Schimmelcultures on 12 Mar., 1996, under the deposition number CBS 284.96, all under the Budapest Treaty.
  • the enzyme is also obtainable from the unidentified fungal, mitosporic, coleomycetous deposited at Centraalbureau voor Schimmelcultures on 12 Mar. 1996, under the deposition numbers CBS 270.96, CBS 271.96 and CBS 273.96, respectively, under the Budapest Treaty.
  • the enzyme is also obtainable from a strain belonging to the group consisting of the genera Cylindrocarpon, Gliocladium, Nectria, Volutella, Sordaria, Scytalidium Thielavia, Syspastospora, Cladorrhinum, Chaetomium, Myceliphthora and Acremonium , especially from a strain belonging to the group consisting of the species Cylindrocarpon sp., Nectria pinea, Volutella colletotrichoides, Sordaria fimicola, Sordaria macrospora, Thielavia terrestris, Thielavia thermophila, Syspastospora boninensis, Cladorrhinum foecundissimum, Chaetomium murorum, Chaetomium virescens, Chaetomium brasiliensis, Chaetomium cunicolorum, Myceliophthora thermophila, Gliocladium catenulatum
  • Nectria pinea was deposited at Centraalbureau voor Schimmelcultures on 12 Mar., 1996, under the deposition number CBS 279.96, and Acremonium Chaetomium sp. was deposited on 28 Sep. 1994 under the deposition number CBS 478.94, both according to the Budapest Treaty.
  • the enzyme is also obtainable from a strain belonging to the group consisting of the species Fusarium solani, Fusarium anguioides, Fusarium poae, Fusarium oxysporum sp. lycopersici, Fusarium oxysporum ssp.
  • Humicola grisea passesiflora, Humicola nigrescens and Humicola grisea , especially Fusarium oxysporum ssp lycopersici CBS 645.78, Fusarium oxysporum ssp passiflora , CBS 744.79, Fusarium solani , IMI 107.511 , Fusarium anguioides , IFO 4467, Fusarium poae , ATCC 60883 , Humicola nigrescens , CBS 819.73 and Humicola grisea , ATCC 22726. It is to be noted that Humicola grisea is different from Humicola grisea var. thermoidea.
  • the enzyme preparation of the invention is derived from the disclosed classes, orders, families, genera and species and essentially consists of an enzyme comprising a first peptide consisting of 13 amino acid residues having the following sequence
  • the amino acid residue at position 9 of the first sequence is selected from the group consisting of proline, threonine, valine, alanine, leucine, isoleucine, phenylalanine, glycine, cysteine, asparagine, glutamine, tyrosine, serine, methionine and tryptophan, more preferably from the group consisting of proline and threonine;
  • the amino acid residue at position 10 of the first sequence which is selected from the group consisting of proline, threonine, valine, alanine, leucine, isoleucine, phenylalanine, glycine, cysteine, asparagine, glutamine, tyrosine, serine, methionine and tryptophan, preferably serine;
  • the amino acid residue at position 12 of the first sequence is selected from the group consisting of proline, threonine, valine, alanine, leucine, isoleucine, phen
  • the present invention provides a DNA construct comprising a DNA sequence encoding an enzyme exhibiting endoglucanase activity, which DNA sequence comprises
  • i) is homologous with the DNA sequence of SEQ ID NO: 1, 7, 9, 11, 13, 15, 21, or 25, respectively, or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae DSM 9770, DSM 10082, DSM 10080, DSM 10081 Escherichia coli DSM 10512, DSM 10511, DSM 10571, DSM 10576, respectively,
  • ii) hybridizes with the same oligonucleotide probe as the DNA sequence of SEQ ID NO: 1, 7, 9, 11, 13, 15, 21, or 25 respectively, or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae DSM 9770, DSM 10082, DSM 10080, DSM 10081 , Escherichia coli , DSM 10512, DSM 10511, DSM 10571, DSM 10576, respectively,
  • iii) encodes a polypeptide which is homologous with the polypeptide encoded by a DNA sequence comprising the DNA sequence of SEQ ID NO: 1, 7, 9, 11, 13, 15, 21, or 25 respectively, or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae DSM 9770, DSM 10082, DSM 10080 DSM 10081 , Escherichia coli , DSM 10512, DSM 10511, DSM 10571, DSM 10576, respectively,
  • iv) encodes a polypeptide which is immunologically reactive with an antibody raised against the purified endoglucanase encoded by the DNA sequence of SEQ ID NO: 1, 7, 9, 11, 13, 15, 21, or 25, respectively, or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae DSM 9770, DSM 10082, DSM 10080, DSM 10081 , Escherichia coli , DSM 10512, DSM 10511, DSM 10571, DSM 10576, respectively.
  • Escherichia coli DSM 10512 was deposited under the Budapest Treaty on 2 Feb., 1996, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany).
  • Escherichia coli DSM 10511 was deposited under the Budapest Treaty on 2 Feb., 1996, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany).
  • Escherichia coli DSM 10571 was deposited under the Budapest Treaty on 6 Mar., 1996, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany).
  • Escherichia coli DSM 10576 was deposited under the Budapest Treaty on 12 Mar., 1996, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, to D-38124 Braunschweig, Germany).
  • Escherichia coli DSM 10583 was deposited under the Budapest Treaty on 13 Mar., 1996, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany).
  • Escherichia coli DSM 10584 was deposited under the Budapest Treaty on 13 Mar., 1996, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany).
  • Escherichia coli DSM 10585 was deposited under the Budapest Treaty on 13 Mar., 1996, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder leg 16, D-38124 Braunschweig, Germany).
  • Escherichia coli DSM 10586 was deposited under the Budapest Treaty on 13 Mar., 1996, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany).
  • Escherichia coli DSM 10587 was deposited under the Budapest Treaty on 13 Mar., 1996, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder leg 16, D-38124 Braunschweig, Germany).
  • Escherichia coli DSM 10588 was deposited under the Budapest Treaty on 13 Mar., 1996, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany).
  • Saccharomyces cerevisiae DSM 9770 was deposited under the Budapest Treaty on 24 Feb., 1995, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany).
  • Saccharomyces cerevisiae DSM 10082 was deposited under the Budapest Treaty on 30 Jun., 1995, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany).
  • Saccharomyces cerevisiae DSM 10080 was deposited under the Budapest Treaty on 30 Jun., 1995, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany).
  • Saccharomyces cerevisiae DSM 10081 was deposited under the Budapest Treaty on 30 Jun., 1995, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany).
  • the DNA construct of the invention relating to SEQ ID NO: 1 can be isolated from or produced on the basis of a DNA library of a strain of Myceliophthora , in particular a strain of M. thermophila , especially M. thermophila , CBS 117.65.
  • the DNA constructs of the invention relating to SEQ ID NOS: 7 and 9 can be isolated from or produced on the basis of a DNA library of a strain of Acrermonium , especially Acremonium sp., CBS 478.94.
  • the DNA construct of the invention relating to SEQ ID NO: 11 can be isolated from or produced on the basis of a DNA library of a strain of Thielavia in particular a strain of Thielavia terrestris , especially Thielavia terrestris , NRRL 8126.
  • the DNA construct of the invention relating to SEQ ID NO-13 can be isolated from or produced on the basis of a DNA library of a strain of Macrophomina , in particular a strain of M. phaseolina , especially M. phaseolina , CBS 281.96
  • the DNA construct of the invention relating to SEQ ID NO: 15 can be isolated from or produced on the basis of a DNA library of a strain of Crinipellis , in particular a strain of C. scabella , especially C. scabella , CBS 280.96.
  • the DNA construct of the invention relating to SEQ ID NO: 25 can be isolated from or produced on the basis of a DNA library of a strain of Sordaria , in particular a strain of Sordaria fimicola.
  • the “analogue” of the DNA sequence of SEQ ID NO: 1, 7, 9, 11, 13, 15, 21, or 25, respectively is intended to indicate any DNA sequence encoding an enzyme exhibiting endoglucanase activity, which has any or all of the properties i)-iv).
  • the analogous DNA sequence is intended to indicate any DNA sequence encoding an enzyme exhibiting endoglucanase activity, which has any or all of the properties i)-iv).
  • a) may be isolated from another or related (e.g., the same) organism producing the enzyme with endoglucanase activity on the basis of the DNA sequence of SEQ ID NO: 1, 7, 9, 11, 13, 15, 21, or 25, respectively, e.g., using the procedures described herein;
  • the homologue may be an allelic variant of the DNA sequence comprising the DNA sequences shown herein, i.e., an alternative form of a gene that arises through mutation; mutations can be silent (no change in the encoded enzyme) or may encode enzymes having altered amino acid sequence;
  • the homologue of the present DNA sequence may also be a genus or species homologue, i.e., encoding an enzyme with a similar activity derived from another species,
  • b) may be constructed on the basis of the DNA sequences of SEQ ID NO: 1, 7, 9, 11, 13, 15, 21, or 25, respectively, e.g., by introduction of nucleotide substitutions which do not give rise to another amino acid sequence of the endoglucanase encoded by the DNA sequence, but which correspond to the codon usage of the host organism intended for production of the enzyme, or by introduction of nucleotide substitutions which may give rise to a different amino acid sequence.
  • amino acid changes are preferably of a minor nature, that is conservative amino acid substitutions that do not significantly affect the folding or activity of the protein, small deletions, typically of one to about 30 amino acid; small amino- or carboxyl-terminal extensions, such as an amino-terminal methionine residue, a small tinker peptide of up to about 20 residues, or a small extension that facilitates purification, such as a poly-histidine tract, an antigenic epitope or a binding domain.
  • Examples of conservative substitutions are within the group of basic amino acids (such as arginine, lysine, histidine), acidic amino acids (such as glutamic acid and aspartic acid), polar amino acids (such as glutamine and asparagine), hydrophobic amino acids (such as leucine, isoleucine, valine), aromatic amino acids (such as phenylalanine, tryptophan, tyrosine) and small amino acids (such as glycine, alanine, serine, threonine, methionine).
  • basic amino acids such as arginine, lysine, histidine
  • acidic amino acids such as glutamic acid and aspartic acid
  • polar amino acids such as glutamine and asparagine
  • hydrophobic amino acids such as leucine, isoleucine, valine
  • aromatic amino acids such as phenylalanine, tryptophan, tyrosine
  • small amino acids such as glycine, alanine, serine
  • amino acids essential to the activity of the polypeptide encoded by the DNA construct of the invention may be identified according to procedures known in the art, such as site-directed mutagenesis or alanine-scanning mutagenesis (Cunningham and Wells, Science 244: 1081-1085 (1989)). In the latter technique mutations are introduced at every residue in the molecule, and the resultant mutant molecules are tested for biological (i.e., endoglucanase) activity to identify amino acid residues that are critical to the activity of the molecule.
  • Sites of substrate-enzyme interaction can also be determined by analysis of crystal structure as determined by such techniques as nuclear magnetic resonance, crystallography or photoaffinity labeling, See, for example, de Vos et al., Science 255: 306-312 (1992); Smith et al. J. Mol. Biol. 224: 899-904 (1992); /Wlodaver et al., FEBS Lett. 309: 59-64 (1992).
  • the endoglucanase encoded by the DNA sequence of the DNA construct of the invention may comprise a cellulose binding domain (CBD) existing as an integral part of the encoded enzyme, or a CBD from another origin may be introduced into the endoglucanase enzyme thus creating an enzyme hybride.
  • CBD cellulose binding domain
  • the term “cellulose-binding domain” is intended to be understood as defined by Peter Tomme et al. “Cellulose-Binding Domains, Classification and Properties” in “Enzymatic Degradation of Insoluble Carbohydrates”, John N. Saddler and Michael H. Penner (Eds.), ACS Symposium Series, No. 618, 1996.
  • CBDs cellulose-binding domains
  • I-X cellulose-binding domains
  • CBDs are found in various enzymes such as cellulases, xylanases, mannanases, arabinofuranosidases, acetyl esterases and chitinases.
  • CBDs have also been found in algae, e.g., the red alga Porphyra purpurea as a non-hydrolytic polysaccharide-binding protein, for reference see Peter Tomme et al., supra.
  • Enzyme hybrids are known in the art, see, e.g., WO 90/00609 and WO 95/16782, and may be prepared by transforming into a host cell a DNA construct comprising at least a fragment of DNA encoding the cellulose-binding domain ligated, with or without a linker, to a DNA sequence encoding the enzyme of interest and growing the host cell to express the fused gene. Enzyme hybrids may be described by the following formula,
  • CBD is the N-terminal or the C-terminal region of an amino acid sequence corresponding to at least the cellulose-binding domain
  • MR is the middle region (the linker), and may be a bond, or a short linking group preferably of from about 2 to about 100 carbon atoms, more preferably of from 2 to 40 carbon atoms; or is preferably from about 2 to about 100 amino acids, more preferably of from 2 to 40 amino acids
  • X is an N-terminal or C-terminal region of a polypeptide encoded by the DNA sequence of the invention.
  • the homology referred to in i) above is determined as the degree of identity between the two sequences indicating a derivation of the first sequence from the second,
  • the homology may suitably be determined by means of computer programs known in the art such as GAP provided in the GCG program package (Needleman, S. B. and Wunsch, C. D., Journal of Molecular Biology, 48: 443-453, 1970).
  • the coding region of the DNA sequence exhibits a degree of identity preferably of at least 60%, more preferably at least 65%, more preferably at least 70%, even more preferably at least 80%, especially at least 90%, with the coding region of the DNA sequence of SEQ ID NO: 1, 7, 9, 11, 13, 15, or 21, respectively, or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae , DSM 9770, DSM 10082, DSM 10080, DSM 10081 , Escherichia coli DSM 10512, DSM 10511, DSM 10571, or DSM 10576, respectively.
  • hybridization referred to in ii) above is intended to indicate that the analogous DNA sequence hybridizes to the same probe as the DNA sequence encoding the endoglucanase enzyme under certain specified conditions which are described in detail in the Materials and Methods section hereinafter.
  • the oligonucleotide probe to be used is the DNA sequence corresponding to the endoglucanase encoding part of the DNA sequence of SEQ ID NO: 1, 7, 9, 11, 13, 15 or 21, respectively, or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae , DSM 9770, DSM 10082, DSM 10080, DSM 10081 , Escherichia coli , DSM 10512, DSM 10511, DSM 10571 or DSM 10576, respectively.
  • the homology referred to in iii) above is determined as the degree of identity between the two sequences indicating a derivation of the first sequence from the second.
  • the homology may suitably be determined by means of computer programs known in the art such as GAP provided in the GCG program package (Needleman, S. B. and Wunsch, C. D., Journal of Molecular Biology, 48: 443-453, 1970).
  • the polypeptide encoded by an analogous DNA sequence exhibits a degree of identity preferably of at least 55%, more preferably at least 80%, more preferably at least 858%, even more preferably at least 70%, more preferably at least 80%, especially at least 90%, with the enzyme encoded by a DNA construct comprising the DNA sequence of SEQ ID NO: 1, 7, 9, 11, 13, 15, 21, or 25, respectively, or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae , DSM 9770, DSM 10082, DSM 10080, DSM 10081, Escherichia coli , DSM 10512, DSM 10511, DSM 10571 or DSM 10576, respectively.
  • the invention relates to an expression vector harbouring a DNA construct of the invention, a cell comprising the DNA construct or expression vector and a method of producing an enzyme exhibiting endoglucanase activity which method comprises culturing said cell under conditions permitting the production of the enzyme, and recovering the enzyme from the culture.
  • the invention relates to an enzyme exhibiting endoglucanase activity, which enzyme
  • a) is encoded by a DNA construct of the invention
  • c) is immunologically reactive with an antibody raised against a purified endoglucanase encoded by the DNA sequence of SEQ ID NO: 1, 7, 9, 11, 13, 15, or 21, respectively, or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae , DSM 9770, DSM 10082, DSM 10080, DSM 10081, Escherichia coli , DSM 10512, DSM 10511, DSM 10571 or DSM 10576, respectively.
  • the endoglucanase mentioned in c) above may be encoded by the DNA sequence isolated from the strain Saccharomyces cerevisiae , DSM 9770, DSM 10082, DSM 10080, DSM 10081, Escherichia coli , DSM 10512, DSM 10511, DSM 10571 or DSM 10576, respectively, and produced in a host organism transformed with said DNA sequence or the corresponding endoglucanase naturally produced by Myceliophthora thermophila, Acremonium sp., Thielavia terrestris, Macrophomina phaseolina, Crinipellis scabella, Volutella colletotrichoides or Sordaria fimicola , respectively.
  • enzyme is understood to include a mature protein or a precursor form thereof as well to a functional fragment thereof which essentially has the activity of the full-length enzyme. Furthermore, the term “enzyme” is intended to include homologues of said enzyme.
  • Homologues of the present enzyme may have one or more amino acid substitutions, deletions or additions. These changes are preferably of a minor nature, that is conservative amino acid substitutions that do not significantly affect the folding or activity of the protein, small deletions, typically of one to about 30 amino acids; small amino- or carboxyl-terminal extensions, such as an amino-terminal methionine residue, a small linker peptide of up to about 20-25 residues, or a small extension that facilitates purification, such as a poly-histidine tract, an antigenic epitope or a binding domain. See in general Ford et al., Protein Expression and Purification 2: 95-107 (1991).
  • conservative substitutions are within the group of basic amino acids (such as arginine, lysine, histidine), acidic amino acids (such as glutamic acid and aspartic acid), polar amino acids (such as glutamine and asparagine), hydrophobic amino acids (such as leucine, isoleucine, valine), aromatic amino acids (such as phenylalanine, tryptophan, tyrosine) and small amino acids (such as glycine, alanine, serine, threonine, methionine).
  • basic amino acids such as arginine, lysine, histidine
  • acidic amino acids such as glutamic acid and aspartic acid
  • polar amino acids such as glutamine and asparagine
  • hydrophobic amino acids such as leucine, isoleucine, valine
  • aromatic amino acids such as phenylalanine, tryptophan, tyrosine
  • small amino acids such as glycine, alanine, serine,
  • substitutions can be made outside the regions critical to the function of the molecule and still result in an active enzyme.
  • Amino acids essential to the activity of the enzyme of the invention may be identified according to procedures known in the art, such as site-directed mutagenesis or alanine-scanning mutagenesis (Cunningham, 1989). In the latter technique mutations are introduced at every residue in the molecule, and the resultant mutant molecules are tested for cellulytic activity to identify amino acid residues that are critical to the activity of the molecule.
  • Sites of ligand-receptor interaction can also be determined by analysis of crystal structure as determined by such techniques as nuclear magnetic resonance, crystallography or photoaffinity labelling. See, for example, de Vos et al., 1992; Smith et al., 1992, Wlodaver et al., 1992.
  • the homologue may be an allelic variant, i.e., an alternative form of a gene that arises through mutation, or an altered enzyme encoded by the mutated gene, but having substantially the same activity as the enzyme of the invention. Hence mutations can be silent (no change in the encoded enzyme) or may encode enzymes having altered amino acid sequence.
  • the homologue of the present enzyme may also be a genus or species homologue, i.e. an enzyme with a similar activity derived from another species.
  • a homologue of the enzyme may be isolated by using the procedures described herein.
  • Molecular screening for DNA sequences of the invention may be carried out by polymerase chain reaction (PC R) using genomic DNA or double-stranded cDNA isolated from a suitable source, such as any of the herein mentioned organisms, and synthetic oligonucleotide primers prepared on the basis of the DNA sequences or the amino acid sequences disclosed herein.
  • a suitable source such as any of the herein mentioned organisms
  • synthetic oligonucleotide primers prepared on the basis of the DNA sequences or the amino acid sequences disclosed herein.
  • it suitable oligonucleotide primers may be the primers described in the Materials and Methods section.
  • the PCR fragment generated in the molecular screening may be isolated and subcloned into a suitable vector.
  • the PCR fragment may be used for screening DNA libraries by, e.g., colony or plaque hybridization.
  • DNA sequence of the invention encoding an enzyme exhibiting endoglucanase activity may be isolated by a general method involving
  • the DNA sequence coding for the enzyme may for instance be isolated by screening a cDNA library of Macrophomina phaseolina. Crinipellis scabella, Sordaria fimicola or Volutella colletotrichoides , and selecting for clones expressing the appropriate enzyme activity (i.e., endoglucanase activity) or from Escherichia coli DSM 10512 deposited under the Budapest Treaty on 2 Feb. 1996, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany), or from Escherichia coli DSM 10511 deposited under the Budapest Treaty on 2 Feb.
  • nucleic acid construct is intended to indicate any nucleic acid molecule of cDNA, genomic DNA, synthetic DNA or RNA origin.
  • construct is intended to indicate a nucleic acid segment which may be single or double-stranded, and which may be based on a complete or partial naturally occurring nucleotide sequence encoding an enzyme of interest.
  • the construct may optionally contain other nucleic acid segments.
  • the nucleic acid construct encoding the enzyme of the invention may suitably be of genomic or cDNA origin, for instance obtained by preparing a genomic or cDNA library and screening for DNA sequences coding for all or part of the enzyme by hybridization using synthetic oligonucleotide probes in accordance with standard techniques (cf. Sambrook et al., 1989).
  • the nucleic acid construct encoding the enzyme may also be prepared synthetically by established standard methods, e.g., the phosphoramidite method described by Beaucage and Caruthers (1981), or the method described by Matthes et al. (1984).
  • the phosphoramidite method oligonucleotides are synthesized, e.g., in an automatic DNA synthesizer, purified, annealed, ligated and cloned in suitable vectors.
  • nucleic acid construct may be of mixed synthetic and genomic, mixed synthetic and cDNA or mixed genomic and cDNA origin prepared by ligating fragments of synthetic, genomic or cDNA origin (as appropriate), the fragments corresponding to various parts of the entire nucleic acid construct, in accordance with standard techniques.
  • the nucleic acid construct may also be prepared by polymerase chain reaction using specific primers, for instance as described in U.S. Pat. No. 4,683,202 or Saiki et al. (1988).
  • the nucleic acid construct is preferably a DNA construct which term will be used exclusively in this specification and claims,
  • a recombinant vector comprising a DNA construct encoding the enzyme of the invention may be any vector which may conveniently be subjected to recombinant DNA procedures, and the choice of vector will often depend on the host cell into which it is to be introduced.
  • the vector may be an autonomously replicating vector, i.e., a vector which exists as an extrachromosomal entity, the replication of which is independent of chromosomal replication, e.g., a plasmid.
  • the vector may be one which, when introduced into a host cell, is integrated into the host cell genome and replicated together with the chromosome(s) into which it has been integrated.
  • the vector is preferably an expression vector in which the DNA sequence encoding the enzyme of the invention is operably linked to additional segments required for transcription of the DNA
  • the expression vector is derived from plasmid or viral DNA, or may contain elements of both.
  • operably linked indicates that the segments are arranged so that they function in concert for their intended purposes, e.g., transcription initiates in a promoter and proceeds through the DNA sequence coding for the enzyme.
  • the promoter may be any DNA sequence which shows transcriptional activity in the host cell of choice and may be derived from genes encoding proteins either homologous or heterologous to the host cell.
  • promoters for use in yeast host cells include promoters from yeast glycolytic genes (Hitzeman et al., J. Biol. Chem. 255: 12073-12080 (1980); Alber and Kawasaki, J. Mol. Appl. Gen. 1: 419-434 (1982)) or alcohol dehydrogenase genes (Young et al., in Genetic Engineering of Microorganisms for Chemicals (Hollaender et at, eds.), Plenum Press, New York, 1982), or the TPI1 (U.S. Pat. No. 4,599,311) or ADH2-4c (Russell et al., Nature 304: 652-654 (1983)) promoters.
  • suitable promoters for use in filamentous fungus host cells are, for instance, to the ADH3 promoter (McKnight et a ⁇ . The EMBO J. 4: 2093-2099 (1985)) or the tpiA promoter.
  • suitable promoters are those derived from the gene encoding A. oryzae TAKA amylase, Rhizomucor miehei aspartic proteinase, A. niger neutral alpha-amylase, A. niger acid stable alpha-amylase, A. niger or A. awamori glucoamylase (gluA), Rhizomucor miehei lipase, A. oryzae alkaline protease, A. oryzae triose phosphate isomerase or A. nidulans acetamidase.
  • Preferred are the TAKA-amylase and gluA promoters.
  • suitable promoters for use in bacterial host cells include the promoter of the Bacillus stearothermophilus maltogenic amylase gene, the Bacillus licheniformis alpha-amylase gene, the Bacillus amyloliquefaciens BAN amylase gene, the Bacillus subtilis alkaline protease gen, or the Bacillus pumilus xylosidase gene, or by the phage Lambda P R or P L promoters or the E. coli lac, trp, or tac promoters.
  • the DNA sequence encoding the enzyme of the invention may also, if necessary, be operably connected to a suitable terminator.
  • the recombinant vector of the invention may further comprise a DNA sequence enabling the vector to replicate in the host cell in question.
  • the vector may also comprise a selectable marker, e.g., a gene the product of which complements a defect in the host cell, such as the gene coding for dihydrofolate reductase (DHFR) or the Schizosaccharomyces pombe TPI gene (described by P. R. Russell, Gene 40, 1985, pp. 125-130).
  • selectable markers include amdS pyrG, argB, niaD, sC.
  • a secretory signal sequence (also known as a leader sequence, prepro sequence or pre sequence) may be provided in the recombinant vector.
  • the secretory signal sequence is joined to the DNA sequence encoding the enzyme in the correct reading frame.
  • Secretory signal sequences are commonly positioned 5′ to the DNA sequence encoding the enzyme,
  • the secretory signal sequence may be that normally associated with the enzyme or may be from a gene encoding another secreted protein.
  • the secretory signal sequence may encode any signal peptide which ensures efficient direction of the expressed enzyme into the secretory pathway of the cell.
  • the signal peptide may be a naturally occurring signal peptide, or a functional part thereof, or it may be a synthetic peptide. Suitable signal peptides have been found to be the alpha-factor signal peptide (cf. U.S. Pat. No. 4,870,008), the signal peptide of mouse salivary amylase (cf. O. Hagenbuchle et al., Nature 289, 1981, pp. 643-646), a modified carboxypeptidase signal peptide (cf. L. A. Valls et al., Cell 48, 1987, pp.
  • yeast BAR1 signal peptide cf. WO 87/02670
  • yeast aspartic protease 3 YAP3
  • a sequence encoding a leader peptide may also be inserted downstream of the signal sequence and upstream of the DNA sequence encoding the enzyme,
  • the function of the leader peptide is to allow the expressed enzyme to be directed from the endoplasmic reticulum to the Golgi apparatus and further to a secretory vesicle for secretion into the culture medium (i.e., exportation of the enzyme across the cell wall or at least through the cellular membrane into the periplasmic space of the yeast cell).
  • the leader peptide may be the yeast alpha-factor leader (the use of which is described in, e.g., U.S. Pat. No.
  • the leader peptide may be a synthetic leader peptide, which is to say a leader peptide not found in nature. Synthetic leader peptides may, for instance, be constructed as described in WO 89102463 or WO 92/11378.
  • the signal peptide may conveniently be derived from a gene encoding an Aspergillus sp. amylase or glucoamylase, a gene encoding a Rhizomucor miehei lipase or protease, a Humicola lanuginosa lipase.
  • the signal peptide is preferably derived from a gene encoding A. oryzae TAKA amylase, A. niger neutral alpha-amylase, A. niger acid-stable amylase, or A. niger glucoamylase.
  • the DNA sequence encoding the present enzyme introduced into the host cell may be either homologous or heterologous to the host in question. If homologous to the host cell, i.e., produced by the host cell in nature, it will typically be operably connected to another promoter sequence or, if applicable, another secretory signal sequence and/or terminator sequence than in its natural environment.
  • the term “homologous” is intended to include a cDNA sequence encoding an enzyme native to the host organism in question.
  • heterologous is intended to include a DNA sequence not expressed by the host cell in nature. Thus, the DNA sequence may be from another organism, or it may be a synthetic sequence.
  • the host cell into which the DNA construct or the recombinant vector of the invention is introduced may be any cell which is capable of producing the present enzyme and includes bacteria, yeast, fungi and higher eukaryotic cells.
  • Examples of bacterial host cells which, on cultivation, are capable of producing the enzyme of the invention are gram-positive bacteria such as strains of Bacillus , such as strains of B. subtilis, B. licheniformis, B. lentus, B. brevis, B. stearothermophilus, B. alkalophilus, B. amyloliquefaciens.
  • B. coagulans, B. circulans, B. lautus, B. megatherium or B. thuringiensis or strains of Streptomyces , such as S. lividans or S. murinus , or gram-negative bacteria such as Echerichia coli .
  • the transformation of the bacteria may be effected by protoplast transformation or by using competent cells in a manner known per se (cf. Sambrook et al., supra).
  • the enzyme When expressing the enzyme in bacteria such as E. coli , the enzyme may be retained in the cytoplasm, typically as insoluble granules (known as inclusion bodies), or may be directed to the periplasmic space by a bacterial secretion sequence. In the former case, the cells are lysed and the granules are recovered and denatured after which the enzyme is refolded by diluting the denaturing agent. In the latter case, the enzyme may be recovered from the periplasmic space by disrupting the cells, e.g., by sonication or osmotic shock, to release the contents of the periplasmic space and recovering the enzyme.
  • yeasts cells include cells of Saccharomyces spp. or Schizosacharomyces spp., in particular strains of Saccharomyces cerevisiae or Saccharomyces kluyveri .
  • Methods for transforming yeast cells with heterologous DNA and producing heterologous enzymes therefrom are described, e.g., in U.S. Pat. No. 4,599,311, U.S. Pat. No. 4,931,373, U.S. Pat. Nos. 4,870,008, 5,037,743, and U.S. Pat. No. 4,845,075, all of which are hereby incorporated by reference.
  • Transformed cells are selected by a phenotype determined by a selectable marker, commonly drug resistance or the ability to grow in the absence of a particular nutrient, e.g., leucine.
  • a preferred vector for use in yeast is the POT1 vector disclosed in U.S. Pat. No. 4,931,373.
  • the DNA sequence encoding the enzyme of the invention may be preceded by a signal sequence and optionally a leader sequence, e.g., as described above.
  • suitable yeast cells are strains of Kluyveromyces such as K. lactis, Hansenula , e.g., H. polymorpha , or Pichia , e.g., P. pastoris (cf. Gleeson et al., S. Gen. Microbiol. 132, 1986, pp. 3459-3465; U.S. Pat. No. 4,882,279).
  • Examples of other fungal cells are cells of filamentous fungi, e.g., Aspergillus spp., Neurospora spp., Fusarium spp. or Trichoderma spp., in particular strains of A. oryzae, A. nidulans, A. niger , or Fusarium graminearum .
  • Aspergillus spp. for the expression of proteins is described in, e.g., EP 272 277, ER 230 023.
  • the transformation of F. oxysporum may, for instance, be carried out as described by Malardier et al., 1989, Gene 78; 147-156.
  • a filamentous fungus When a filamentous fungus is used as the host cell, it may be transformed with the DNA construct of the invention, conveniently by integrating the DNA construct in the host chromosome to obtain a recombinant host cell.
  • This integration is generally considered to be an advantage as the to DNA sequence is more likely to be stably maintained in the cell. Integration of the DNA constructs into the host chromosome may be performed according to conventional methods, e.g., by homologous or heterologous recombination.
  • the transformed or transfected host cell described above is then cultured in a suitable nutrient medium under conditions permitting the expression of the present enzyme, after which the resulting enzyme is recovered from the culture.
  • the medium used to culture the cells may be any conventional medium suitable for growing the host cells, such as minimal or complex media containing appropriate supplements. Suitable media are available from commercial suppliers or may be prepared according to published recipes (e.g., in catalogues of the American Type Culture Collection).
  • the enzyme produced by the cells may then be recovered from the culture medium by conventional procedures including separating the host cells from the medium by centrifugation or filtration, precipitating the proteinaceous components of the supernatant or filtrate by means of a salt, e.g., ammonium sulphate, purification by a variety of chromatographic procedures, e.g., ion exchange chromatography, ge filtration chromatography, affinity chromatography, or the like, dependent on the type of enzyme in question.
  • a salt e.g., ammonium sulphate
  • the present invention relates to a method of producing an enzyme according to the invention, wherein a suitable host cell transformed with a DNA sequence encoding the enzyme is cultured under conditions permitting the production of the enzyme, and the resulting enzyme is recovered from the culture.
  • a taxonomic hot spot for production of cellulytic enzymes can further only be fully explored if also the ecological approach is included.
  • Thorough knowledge about the adaptation to various substrates are prerequisites for designing an intelligent screening and for managing a successful selection of strains and ecological niches to be searched.
  • the screening and cloning may be carried out using the following:
  • Leaf of Camellia japonica (Theaceae, Guttiferales), grown in Kunming Botanical garden, Yunnan Province, China Classification: Ascomycota, Loculoascomycetes, Dothideales, (family unclassified)
  • Rhizophlyctis rosea (de Bary & Wor) Fischer
  • Rhizomucor pusillus (Lindt) Schipper
  • Escherichia coli MC1061 and DH10B Escherichia coli MC1061 and DH10B.
  • Yeast strain The Saccharomyces cerevisiae strain used was W3124 (MAT ⁇ ; ura 3-52, leu 2-3, 112, h is 3-D200; pep 4-1137; prc1; HIS3; prb2: LEU2; cir+).
  • the Aspergillus expression vector pH D414 is a derivative of the plasmid p775 (described in EP 238 023). The construction of pHD414 is further described in WO 93/11249.
  • pYES 2.0 (Invitrogen) pA2C477, pA2C193, pA2C357, pA2C371, pA2C385, pA2C475, pA2C488, pA2C502 (See example 1, 2, 3 and 4),
  • the full length DNA sequence comprising the cDNA sequence of SEC ID NO: 1, 7, 9, 11, 13, 15, 21, or 25, respectively, coding for the endoglucanase of the invention, can be obtained from the deposited organism S. cerevisiae , DSM 9770, DSM 10082, DSM 10080, DSM 10081, E. coli , DSM 10512, DSM 10511, DSM 10571 or DSM 10576, respectively, by extraction of plasmid DNA by methods known in the art (Sambrook et al. (1989) Molecular cloning: A laboratory manual, Cold Spring Harbor lab., Cold Spring Harbor, N.Y.).
  • the four degenerate, deoxyinosine-containing oligonucleotide primers (sense; s and antisense; as1, as2 and as3) corresponding to four highly conserved amino acid regions found in the deduced amino acid sequences of Thielavia terrestris cellulase, Myceliophthora thermophilum cellulase, and two cellulases from Acremonium sp. The residues are numbered according to the Myceliophthora thermophilum sequence. The deoxyinosines are depicted by an I in the primer sequences, and the restriction sites are underlined.
  • cDNA was synthesized from 5 micrograms of poly(A)+ RNA as described below. Genomic DNA was isolated according to Yelton et al.
  • PCR buffer (10 mM Tris-HCl, pH 8.3, 50 mM KCl, 1.5 mM MgCl 2 , 0.01% (w/v) gelatin) containing 200 micro-M of each dNTP and 100 pmol of each degenerate primer in three combinations,
  • the PCP fragments were recovered by centrifugation, washed in 70% EtOH, dried and resuspended in 20 microliters of restriction enzyme buffer (10 mM Tris-HCl, 10 mM MgCl 2 , 50 mM NaCl, 1 mM DTT).
  • the fragments were digested with HindIII and XbaI, phenol and chloroform extracted, recovered by precipitation with 2 vols of 96% EtOH and 0.1 of 3 M NaAc, pH 5.2, and subcloned into HindIII/XbaI-cleaved pYES 2.0 vector.
  • cDNA libraries in S. cerevisiae or E. coli constructed as described below, were screened by colony hybridization (Sambrook, 1989) using the corresponding random-primed (Feinberg and Vogelstein) 32 P-labeled (>1 ⁇ 10 9 cpm/microgram) PCR products as probes.
  • the hybridizations were carried out in 2 ⁇ SSC (Sambrook, 1989), 5 ⁇ Denhardt's solution (Sambrook, 1989), 0.5% (w/v) SDS, 100 micrograms/ml denatured salmon sperm DNA for 20 h at 65° C. followed by washes in 5 ⁇ SSC at 25° C. (2 ⁇ 15 min), 2 ⁇ SSC 0.5% SDS at 65° C.
  • the positive cDNA clones were characterized by sequencing the ends of the cDNA inserts with pYES 2.0 polylinker primers (Invitrogen, USA), and by determining the nucleotide sequence of the longest cDNA from both strands by the dideoxy chain termination method (Sanger et al.) using fluorescent labeled terminators.
  • Qiagen purified plasmid DNA (Qiagen, USA) was sequenced with the Taq deoxy terminal cycle sequencing kit (Perkin Elmer, USA) and either pYES 2.0 polylinker primers (Invitrogen, USA) or synthetic oligonucleotide primers using an Applied Biosystems 373A automated sequencer according to the manufacturer's instructions. Analysis of the sequence data was performed according to Devereux et al.
  • Double-stranded cDNA was synthesized from 5 micrograms poly(A) + RNA by the RNase H method (Gubler and Hoffman Gene 25:263-269 (1983), Sambrook et al. (1939) Molecular cloning: A laboratory manual, Cold Spring Harbor lab., Cold Spring Harbor, N.Y.) using the hair-pin modification developed by F. S. Hagen (pers. comm.).
  • the poly(A) + RNA (5 micrograms in 5 microliters of DEPC-treated water) was heated at 70° C. for 8 min.
  • First-strand cDNA was synthesized by incubating the reaction mixture at 45° C. for 1 hour. After synthesis, the mRNA:cDNA hybrid mixture was gelfiltrated through a MicroSpin S-400 HR (Pharmadia) spin column according to the manufacturer's instructions.
  • the hybrids were diluted in 250 microliters second strand buffer (20 mM Tris-Cl, pH 7.4, 90 mM KCl, 4.6 mM MgCl 2 , 10 mM (NH 4 ) 2 SO 4 , 0.16 mM ⁇ NAD+) containing 200 micro-M of each dNTP, 60 units E. coli DNA polymerase I (Pharmacia), 5.25 units RNase H (Promega) and 15 units E. coli DNA ligase (Boehringer Mannheim). Second strand cDNA synthesis was performed by incubating the reaction tube at 16° C. for 2 hours and additional 15 min. at 25° C. The reaction was stopped by addition of EDTA to a final concentration of 20 mM followed by phenol x's and chloroform extractions.
  • second strand buffer 20 mM Tris-Cl, pH 7.4, 90 mM KCl, 4.6 mM MgCl 2 , 10 mM (NH 4 ) 2 SO
  • the double-stranded cDNA was precipitated at ⁇ 20° C. for 12 hours by addition of 2 vols 96% EtOH, 0.2 vol 10 M NH 4 Ac, recovered by centrifugation, washed in 70% EtOH, dried and resuspended in 30 microliters Mung bean nuclease buffer (30 mM NaAc, pH 4.6, 300 mM NaCl, 1 mM ZnSO 4 , 0.35 mM DTT, 2% glycerol) containing 25 units Mung bean nuclease (Pharmacia).
  • Mung bean nuclease buffer (30 mM NaAc, pH 4.6, 300 mM NaCl, 1 mM ZnSO 4 , 0.35 mM DTT, 2% glycerol) containing 25 units Mung bean nuclease (Pharmacia).
  • the single-stranded hair-pin DNA was clipped by incubating the reaction at 30° C.
  • the double-stranded cDNAs were recovered by centrifugation and blunt-ended in 30 microliters T4 DNA polymerase buffer (20 mM Tris-acetate, pH 7.9, 10 mM MgAc, 50 mM KAc, 1 mM DTT) containing 0.5 mM of each dNTP and 5 units T4 DNA polymerase (New England Biolabs) by incubating the reaction mixture at 16° C. for 1 hour. The reaction was stopped by addition of EDTA to a final concentration of 20 mM, followed by phenol and chloroform extractions, and precipitation for 12 hours at ⁇ 20° C. by adding 2 vols 96% EtOH and 0.1 vol 3 M NaAc pH 5.2.
  • T4 DNA polymerase buffer 20 mM Tris-acetate, pH 7.9, 10 mM MgAc, 50 mM KAc, 1 mM DTT
  • T4 DNA polymerase New England Biolabs
  • the cDNAs were recovered by centrifugation, washed in 70% EtOH and dried.
  • the cDNA pellet was resuspended in 25 microliters ligation buffer (30 mM Tris-Cl, pH 7.8, 10 mM MgCl 2 , 10 mM DTT, 0.5 mM ATP) containing 2.5 micrograms non-palindromic BstXI adaptors (invitrogen) and 30 units T4 ligase (Promega) and incubated at 15° C. for 12 hours. The reaction was stopped by heating at 65° C. for 20 min. and then cooling on ice for 5 min.
  • the adapted cDNA was digested with Not I restriction enzyme by addition of 20 microliters water, 5 microliters 10 ⁇ Not I restriction enzyme buffer (New England Biolabs) and 50 units Not I (New England Biolabs), followed by incubation for 2.5 hours at 37° C. The reaction was stopped by heating at 65° C. for 10 min.
  • the cDNAs were size-fractionated by gel electrophoresis on a 0.8% SeaPlaque GTG low melting temperature agarose gel (FMC) in 1 ⁇ TBE to separate unligated adaptors and small cDNAs.
  • FMC SeaPlaque GTG low melting temperature agarose gel
  • the cDNA was size-selected with a cut-off at 0.7 kb and rescued from the gel by use of beta-Agarase (New England Biolabs) according to the manufacturer's instructions and precipitated for 12 hours at ⁇ 20° C. by adding 2 vols 96% EtOH and 0.1 vol 3 M NaAc pH 5.2.
  • the directional, size-selected cDNA was recovered by centrifugation, washed in 70% EtOH, dried and resuspended in 30 microliters 10 mM Tris-Cl, pH 7.5, 1 mM EDTA.
  • the cDNAs were desalted by gelfiltration through a MicroSpin S-300 HR (Pharmacia) spin column according to the manufacturers instructions.
  • ligation buffer (30 mM Tris-Cl, pH 7.8, 10 mM MgCl 2 , 10 mM DTT, 0.5 mM ATP) containing 5 microliters double-stranded cDNA (reaction tubes #1 and #2), 15 units T4 ligase (Promega) and 30 ng (tube #1), 40 ng (tube #2) and 40 ng (tube #3, the vector background control) of BstXI-NotI cleaved pYES 2.0 vector.
  • the ligation reactions were performed by incubation at 16° C. for 12 hours, heating at 70° C. for 20 min. and addition of 10 microliters water to each tube.
  • E. coli DH10B cells (Bethesda research Laboratories) as described (Sambrook et al. (1989) Molecular cloning: A laboratory manual, Cold Spring Harbor lab., Cold Spring Harbor, N.Y.). Using the optimal conditions a library was established in E. coli consisting of pools. Each pool was made by spreading transformed E. coli on LB+ampicillin agar plates giving 15.000-30.000 colonies/plate after incubation at 37° C. for 24 hours. 20 ml LB+ampicillin was added to the plate and the cells were suspended herein. The cell suspension was shaked in a 50 ml tube for 1 hour at 37° C. Plasmid DNA was isolated from the cells according to the manufacturer's instructions using QIAGEN plasmid kit and stored at ⁇ 20° C.
  • the agar plates were replica plated onto a set of SC+galactose-uracil agar plates containing 0.1% AZCL HE cellulose. These plates were incubated for 3-7 days at 30° C. Endoglucanase positive colonies were identified as colonies surrounded by a blue halo.
  • the positive clones were obtained as single colonies, the cDNA inserts were amplified directly from the yeast colony using biotinylated polylinker primers, purified by magnetic beads (Dynabead M-280, Dynal) system and characterized individually by sequencing the 5′-end of each cDNA clone using the chain-termination method (Sanger et al. (1977) Proc. Natl. Acad. Sci. U.S.A. 74: 5463-5467) and the Sequence system (United States Biochemical).
  • the nucleotide sequence was determined of the longest cDNA from both strands by the dideoxy chain termination method (Sanger et al.) using fluorescent labeled terminators. Plasmid DNA was rescued by transformation into E. coli as described below. Qiagen purified plasmid DNA (Qiagen, USA) was sequenced with the Taq deoxy terminal cycle sequencing kit (Perkin Elmer, USA) and either pYES 2.0 polylinker primers (Invitrogen, USA) or synthetic oligonucleotide primers using an Applied Biosystems 373A automated sequencer according to the manufacturer's instructions. Analysis of the sequence data was performed according to Devereux et at
  • An endoglucanase-producing yeast colony was inoculated into 20 ml YPD broth in a 50 ml grass test tube. The tube was shaken for 2 days at 30° C. The cells were harvested by centrifugation for 10 min. at 3000 rpm.
  • DNA was isolated according to WO 94/14953 and dissolved in 50 microliters water. The DNA was transformed into E. coli by standard procedures. Plasmid DNA was isolated from E. coli using standard procedures, and analyzed by restriction enzyme analysis. The cDNA insert was excised using appropriate restriction enzymes and ligated into an Aspergillus expression vector.
  • Protoplasts may be prepared as described in WO 95/02043, p. 16, line 21-page 17, line 12, which is hereby incorporated by reference.
  • 100 microliters of protoplast suspension is mixed with 5-25 micrograms of the appropriate DNA in 10 microliters of STC (1.2 M sorbitol, 10 mM Tris-HCl, pH 7.5, 10 mM CaCl 2 .
  • Protoplasts are mixed with p3SR2 (an A. nidulans amdS gene carrying plasmid). The mixture is left at room temperature for 25 minutes.
  • the mixture is left at room temperature for 25 minutes, spun at 2500 g for 15 minutes and the pellet is resuspended in 2 ml of 1.2 M sorbitol. After one more sedimentation the protoplasts are spread on minimal plates (Cove, Biochem. Biophys. Acta 113: 51-56 (1966)) containing 1.0 M sucrose, pH 7.0, 10 mM acetamide as nitrogen source and 20 mM CsCl to inhibit background growth. After incubation for 4-7 days at 37° C. spores are picked and spread for single colonies. This procedure is repeated and spores of a single colony after the second resolution is stored as a defined transformant.
  • Each of the transformants were inoculated in 10 ml YPM and propagated. After 2-5 days of incubation at 37° C., 10 ml supernatant was removed. The endoglucanase activity was identified by AZCL HE cellulose as described above.
  • Hybridization conditions (to be used in evaluating property ii) of the DNA construct of the invention): Suitable conditions for determining hybridization between a nucleotide probe and a homologous DNA or RNA sequence involves presoaking of the filter containing the DNA fragments or RNA to hybridize in 5 ⁇ SSC (standard saline citrate) for 10 min, and prehybridization of the filter in a solution of 5 ⁇ SSC (Sambrook et al. 1989), 5 ⁇ Denhardt's solution (Sambrook et al. 1989), 0.5% SDS and 100 micrograms/ml of denatured sonicated salmon sperm DNA (Sambrook et al.
  • 5 ⁇ SSC standard saline citrate
  • the nucleotide probe to be used in the hybridization is the DNA sequence corresponding to the endoglucanase encoding part of the DNA sequence of SEQ ID NO: 1, 7, 9, 11, 13, 15, 21 or 25, respectively, and/or the DNA sequence obtainable from the plasmid in S. cerevisiae , DSM 9770, DSM 10082, DSM 10080, DSM 10081 , E. coli , DSM 10512, DSM 10511: DSM 10571 or DSM 10576, respectively.
  • Antibodies to be used in determining immunological cross-reactivity may be prepared by use of a purified cellulase. More specifically, antiserum against the cellulase of the invention may be raised by immunizing rabbits (or other rodents) according to the procedure described by N. Axelsen et al. in: A Manual of Quantitative Immunoelectrophoresis , Blackwell Scientific Publications, 1973, Chapter 23, or A. Johnstone and R. Thorpe, Immunochemistry in Practice , Blackwell Scientific Publications, 1982 (more specifically pp. 27-31).
  • Purified immunoglobulins may be obtained from the antisera, for example by salt precipitation ((NH 4 ) 2 SO 4 ), followed by dialysis and ion exchange chromatography, e.g., on DEAE-Sephadex.
  • Immunochemical characterization of proteins may be 2 done either by Qutcherlony double-diffusion analysis (O. Ouchterlony in: Handbook of Experimental Immunology (D. M., Weir, Ed.), Blackwell Scientific Publications, 1967, pp. 655-706), by crossed immunoelectrophoresis (N. Axelsen et al., supra, Chapters 3 and 4), or by rocket immunoelectrophoresis (N. Axelsen et al., Chapter 2).
  • YPD 10 g yeast extract, 20 g peptone, H 2 O to 900 ml. Autoclaved, 100 ml 20% glucose (sterile filtered) added.
  • YPM 10 g yeast extract, 20 g peptone, H 2 O to 900 ml. Autoclaved, 100 ml 20% maltodextrin (sterile filtered) added.
  • SC-URA 100 ml 10 ⁇ Basal salt, 28 ml 20% casamino acids without vitamins, 10 ml 1% tryptophan, H 2 O ad 900 ml, autoclaved, 3.6 ml 5% threonine and 100 ml 20% glucose or 20% galactose added.
  • SC-URA agar SC-URA, 20 g/l agar added.
  • PD agar 39 g potato dextrose agar, DIFCO 0013: add deionized water up to 1000 ml; autoclave (121° C. for 15-20 min).
  • PC agar Potatoes and carrots (grinded, 20 g of each) and water, added up to 1000 ml, are boiled for 1 hr; agar (20 g/l of Merck 1614); autoclave (121° C. for 20 min)
  • PC liquid broth as PC agar but without the Agar
  • PD liquid broth 24 g potato dextrose broth, Difco 0549, deionized water up to 1000 ml; autoclave (121° C. for 15-20 min)
  • PC and PD liquid broth with cellulose add 30 g Solcafloc (Dicacel available from Dicalite-Europe-Nord, 9000 Gent, Belgium) per 1000 ml
  • PB-9 liquid broth 12 g Rofec (Roquette 101-0441) and 24 g glucose are added to 1000 ml water; pH is adjusted to 5.5; 5 ml mineral oil and 5 g CaCO 3 are added per 1000 ml. Autoclave (121° C. for 40 min)
  • YPG liquid broth 4 g yeast extract (Difco 0127), 1 g KH 2 PO 4 (Merck4873), 0.5 g MgSO 4 .7H 2 O Merck 5886, 15 g Dextrose, Roquette 101-0441, 0.1 ml Pluronic (101-3088); deionized water up to 1000 ml; autoclave (20 min at 121° C.)
  • P-stock 13.61 g KH 2 PO 4 ; 13.21 g (NH 4 ) 2 PO 4 , 17.42 g KH 2 PO 4 ; deionized water up to 100 ml
  • Ca/Mg stock 7.35 g CaCl 2 , 2H 2 O, 10.7 g MgCl 2 , 6H 2 O, deionized water up to 100 ml; pH adjusted to 7.0; autoclaving (121° C.; 20 min)
  • colour clarification is meant the partly restoration of the initial colours of fabric or garment throughout multiple washing cycles.
  • de-pilling denotes removing of pills from the fabric surface.
  • soaking liquor denotes an aqueous liquor in which laundry may be immersed prior to being subjected to a conventional washing process.
  • the soaking liquor may contain one or more ingredients conventionally used in a washing or laundering process.
  • washing liquor denotes an aqueous liquor in which laundry is subjected to a washing process, i.e., usually a combined chemical and mechanical action either manually or in a washing machine.
  • the washing liquor is an aqueous solution of a powder or liquid detergent composition.
  • rinsing liquor denotes an aqueous liquor in which laundry is immersed and treated, conventionally immediately after being subjected to a washing process, in order to rinse the laundry, i.e., essentially remove the detergent solution from the laundry.
  • the rinsing liquor may contain a fabric conditioning or softening composition.
  • the laundry subjected to the method of the present invention may be conventional washable laundry.
  • the major part of the laundry is sewn or unsewn fabrics, including knits, wovens, denims, yarns, and toweling, made from cotton, cotton blends or natural or manmade cellulosics (e.g., originating from xylan-containing cellulose fibers such as from wood pulp) or blends thereof.
  • blends are blends of cotton or rayon/viscose with one or more companion material such as wool, synthetic fibers (e.g., polyamide fibers, acrylic fibers, polyester fibers, polyvinyl alcohol fibers, polyvinyl chloride fibers, polyvinylidene chloride fibers, polyurethane fibers, polyurea fibers, aramid fibers), and cellulose-containing fibers (e.g., rayon/viscose, ramie, flax/linen, jute, cellulose acetate fibers, lyocell).
  • companion material such as wool, synthetic fibers (e.g., polyamide fibers, acrylic fibers, polyester fibers, polyvinyl alcohol fibers, polyvinyl chloride fibers, polyvinylidene chloride fibers, polyurethane fibers, polyurea fibers, aramid fibers), and cellulose-containing fibers (e.g., rayon/viscose, ramie, flax/linen, jute,
  • the present endoglucanases may typically be components of a detergent composition. As such, they may be included in the detergent composition in the form of a non-dusting granulate, a stabilized liquid, or protected enzymes. Non-dusting granulates may be produced, e.g., as disclosed in U.S. Pat. Nos.
  • waxy coating materials are poly(ethylene oxide) products (polyethyleneglycol, PEG) with mean molecular weights of 1000 to 20000; ethoxylated nonylphenols having from 16 to 50 ethylene oxide units; ethoxylated fatty alcohols in which the alcohol contains from 12 to 20 carbon atoms and in which there are 15 to 80 ethylene oxide units; fatty alcohols; fatty acids; and mono and di- and triglycerides of fatty acids, Examples of film-forming coating materials suitable for application by fluid bed techniques are given in patent GB 1483591.
  • Liquid enzyme preparations may, for instance, be stabilized by adding a polyol such as propylene glycol, a sugar or sugar alcohol, lactic acid or boric acid according to established methods.
  • a polyol such as propylene glycol, a sugar or sugar alcohol, lactic acid or boric acid according to established methods.
  • Other enzyme stabilizers are welt known in the art.
  • Protected enzymes may be prepared according to the method disclosed in EP 238,216.
  • the detergent composition of the invention may be in any convenient form, e.g., as powder, granules, paste or liquid.
  • a liquid detergent may be aqueous, typically containing up to 70% water and 0-30% organic solvent, or nonaqueous.
  • the detergent composition comprises one or more surfactants, each of which may be anionic, nonionic, cationic, or zwitterionic.
  • the detergent will usually contain 0-50% of anionic surfactant such as linear alkylbenzenesulfonate (LAS), alpha-olefinsulfonate (AOS), alkyl sulfate (fatty alcohol sulfate) (AS), alcohol ethoxysulfate (AEOS or AES), secondary alkanesulfonates (SAS), alpha-sulfo fatty acid methyl esters, alkyl- or alkenylsuccinic acid, or soap.
  • anionic surfactant such as linear alkylbenzenesulfonate (LAS), alpha-olefinsulfonate (AOS), alkyl sulfate (fatty alcohol sulfate) (AS), alcohol ethoxysulfate (AEOS or AES), secondary alkanesulfonates (SAS), alpha-
  • nonionic surfactant such as alcohol ethoxylate (AEO or AE), carboxylated alcohol ethoxylates, nonylphenol ethoxylate, alkylpolyglycoside, alkyldimethylamine oxide, ethoxylated fatty acid monoethanolamide, fatty acid monoethanolamide, or polyhydroxy alkyl fatty acid amide (e.g. as described in WO 92/06154).
  • AEO or AE alcohol ethoxylate
  • carboxylated alcohol ethoxylates such as carboxylated alcohol ethoxylates, nonylphenol ethoxylate, alkylpolyglycoside, alkyldimethylamine oxide, ethoxylated fatty acid monoethanolamide, fatty acid monoethanolamide, or polyhydroxy alkyl fatty acid amide (e.g. as described in WO 92/06154).
  • the detergent composition may additionally comprise one or more other enzymes such as amylase, lipase, cutinase, protease, peroxidase, and oxidase, e.g., laccase.
  • enzymes such as amylase, lipase, cutinase, protease, peroxidase, and oxidase, e.g., laccase.
  • the detergent may contain 1-65% of a detergent builder or complexing agent such as zeolite, diphosphate, triphosphate, phosphonate, citrate, nitrilotriacetic acid (NTA), ethylenediaminetetraacetic acid (EDTA), diethylenetriaminepentaacetic acid (DTMPA), alkyl- or alkenylsuccinic acid, soluble silicates or layered silicates (eg., SKS-6 from Hoechst).
  • a detergent builder or complexing agent such as zeolite, diphosphate, triphosphate, phosphonate, citrate, nitrilotriacetic acid (NTA), ethylenediaminetetraacetic acid (EDTA), diethylenetriaminepentaacetic acid (DTMPA), alkyl- or alkenylsuccinic acid, soluble silicates or layered silicates (eg., SKS-6 from Hoechst).
  • the detergent may also be inbuilt, i.
  • the detergent may comprise one or more polymers.
  • examples are carboxymethylcellulose (CMC), poly(vinylpyrrolidone) (PVP), polyethyleneglycol (PEG), poly(vinyl alcohol) (PVA), polycarboxylates such as polyacrylates, maleic/acrylic acid copolymers and lauryl methacrylate/acrylic acid copolymers.
  • the detergent may contain a bleaching system which may comprise a H 2 O 2 source such as perborate or percarbonate which may be combined with a peracid-forming bleach activator such as tetraacetylethylenediamine (TAED) or Nonanoyloxybenzenesulfonate (NOBS).
  • TAED tetraacetylethylenediamine
  • NOBS Nonanoyloxybenzenesulfonate
  • the bleaching system may comprise peroxyacids of, erg., the amide, imide, or sulfone type.
  • the enzymes of the detergent composition of the invention may be stabilized using conventional stabilizing agents, e.g., a polyol such as propylene glycol or glycerol, a sugar or sugar alcohol, lactic acid, boric acid, or a boric acid derivative such as, e.g., an aromatic borate ester, and the composition may be formulated as described in, e.g., WO 92/19709 and WO 92/19708.
  • a polyol such as propylene glycol or glycerol
  • a sugar or sugar alcohol lactic acid, boric acid, or a boric acid derivative such as, e.g., an aromatic borate ester
  • the detergent may also contain other conventional detergent ingredients such as, e.g., fabric conditioners including clays, foam boosters, suds suppressors, anti-corrosion agents, soil-suspending agents, anti-soil-redeposition agents, dyes, bactericides, optical brighteners, or perfume.
  • fabric conditioners including clays, foam boosters, suds suppressors, anti-corrosion agents, soil-suspending agents, anti-soil-redeposition agents, dyes, bactericides, optical brighteners, or perfume.
  • the pH (measured in aqueous solution at use concentration) will usually be neutral or alkaline, e.g., in the range of 7-11.
  • a detergent composition formulated as a granulate having a bulk density of at least 600 g/l comprising
  • Linear alkylbenzenesulfonate (calculated as acid) 7-12% Alcohol ethoxysulfate (e.g., C 12-18 alcohol, 1-2 EO) 1-4% or alkyl sulfate (e.g., C 16-18 ) Alcohol ethoxylate (e.g., C 14-15 alcohol, 7 EO) 5-9% Sodium carbonate (as Na 2 CO 3 ) 14-20% Soluble silicate (as Na 2 O, 2SiO 2 ) 2-6% Zeolite (as NaA1SiO 4 ) 15-22% Sodium sulfate (as Na 2 SO 4 ) 0-6% Sodium citrate/citric acid (as C 6 H 5 Na 3 O 7 /C 6 H 8 O 7 ) 0-15% Sodium perborate (as NaBO 3 •H 2 O) 11-18% TAED 2-6% Carboxymethylcellulose 0-2% Polymers (e.g., maleic/acrylic acid copolymer, PVP
  • Linear alkylbenzenesulfonate (calculated as acid) 6-11% Alcohol ethoxysulfate (e.g., C 12-18 alcohol, 1-2 EO or 1-3% alkyl sulfate (e.g., C 16-18 ) Alcohol ethoxylate (e.g., C 14-15 alcohol, 7 EO) 5-9% Sodium carbonate (as Na 2 CO 3 ) 15-21% Soluble silicate (as Na 2 O, 2SiO 2 ) 1-4% Zeolite (as NaA1SiO 4 ) 24-34% Sodium sulfate (as Na 2 SO 4 ) 4-10% Sodium citrate/citric acid (as C 6 H 5 Na 3 O 7 /C 6 H 8 O 7 ) 0-15% Carboxymethylcellulose 0-2% Polymers (e.g., maleic/acrylic acid copolymer, PVP, 1-6% PEG) Enzymes (calculated as pure enzyme protein) 0.0001-0.1% Minor ingredients
  • Linear alkylbenzenesulfonate (calculated as acid) 5-9% Alcohol ethoxylate (e.g., C 12-15 alcohol, 7 EO) 7-14% Soap as fatty acid (e.g., C 16-22 fatty acid) 1-3% Sodium carbonate (as Na 2 CO 3 ) 10-17% Soluble silicate (as Na 2 O, 2SiO 2 ) 3-9% Zeolite (as NaA1SiO 4 ) 23-33% Sodium sulfate (as Na 2 SO4) 0-4% Sodium perborate (as NaBO 3 •H 2 O) 8-16% TAED 2-8% Phosphonate (e.g., EDTMPA) 0-1% Carboxymethylcellulose 0-2% Polymers (e.g., maleic/acrylic acid copolymer, PVP, 0-3% PEG) Enzymes (calculated as pure enzyme protein) 0.0001-0.1% Minor ingredients (e.g., suds suppressors, perfume, 0-5%
  • Linear alkylbenzenesulfonate (calculated as acid) 15-21% Alcohol ethoxylate (e.g., C 12-15 alcohol, 7 EO or 12-18% C 12-15 alcohol, 5 EO) Soap as fatty acid (e.g., oleic acid) 3-13% Alkenylsuccinic acid (C 12-14 ) 0-13% Aminoethanol 8-18% Citric acid 2-8% Phosphonate 0-3% Polymers (e.g., PVP, PEG) 0-3% Borate (as B 4 O 7 ) 0-2% Ethanol 0-3% Propylene glycol 8-14% Enzymes (calculated as pure enzyme protein) 0.0001-0.1% Minor ingredients (e.g., dispersants, suds suppressors, 0-5% perfume, optical brightener) 6) An aqueous structured liquid detergent composition comprising
  • Linear alkylbenzenesulfonate (calculated as acid) 15-21% Alcohol ethoxylate (e.g., C 12-15 alcohol, 7 EO, or 3-9% C 12-15 alcohol, 5 EO) Soap as fatty acid (e.g., oleic acid) 3-10% Zeolite (as NaA1SiO 4 ) 14-22% Potassium citrate 9-18% Borate (as B 4 O 7 ) 0-2% Carboxymethylcellulose 0-2% Polymers (e.g., PEG, PVP) 0-3% Anchoring polymers such as, e.g., lauryl 0-3% methacrylate/acrylic acid copolymer; molar ratio 25:1; MW 3800 Glycerol 0-5% Enzymes (calculated as pure enzyme protein) 0.0001-0.1% Minor ingredients (e.g., dispersants, suds suppressors, 0-5% perfume, optical brighteners) 7) A detergent composition formulated as a granulate having
  • Linear alkylbenzenesulfonate (calculated as acid) 8-14% Ethoxylated fatty acid monoethanolamide 5-11% Soap as fatty acid 0-3% Sodium carbonate (as Na 2 CO 3 ) 4-10% Soluble silicate (as Na 2 O, 2SiO 2 ) 1-4% Zeolite (as NaA1SiO 4 ) 30-50% Sodium sulfate (as Na 2 SO 4 ) 3-11% Sodium citrate (as C 6 H 5 Na 3 O 7 ) 5-12% Polymers (e.g., PVP, maleic/acrylic acid copolymer, 1-5% PEG) Enzymes (calculated as pure enzyme protein) 0.0001-0.1% Minor ingredients (e.g., suds suppressors, perfume) 0-5% 9) A detergent composition formulated as a granulate comprising
  • Linear alkylbenzenesulfonate (calculated as acid) 20-32% Alcohol ethoxylate (e.g., C 12-15 alcohol, 7 EO, or 6-12% C 12-15 alcohol, 5 EO) Aminoethanol 2-6% Citric acid 8-14% Borate (as B 4 O 7 ) 1-3% Polymer (e.g., maleic/acrylic acid copolymer, 0-3% anchoring polymer such as, e.g., lauryl methacrylate-/acrylic acid copolymer) Glycerol 3-8% Enzymes (calculated as pure enzyme protein) 0.0001-0.1% Minor ingredients (e.g., hydrotropes, dispersants, 0-5% perfume, optical brighteners) 12)
  • a detergent composition formulated as a granulate having a bulk density of at least 600 gal comprising
  • Anionic surfactant (linear alkylbenzenesulfonate, 25-40% alkyl sulfate, alpha-olefinsulfonate, alpha-sulfo fatty acid methyl esters, alkanesulfonates, soap)
  • Nonionic surfactant e.g., alcohol ethoxylate
  • Sodium carbonate (as Na 2 CO 3 ) 8-25%
  • Soluble silicates (as Na 2 O, 2SiO 2 ) 5-15%
  • Sodium sulfate (as Na 2 SO 4 ) 0-5%
  • Zeolite (as NaA1SiO 4 ) 15-28% Sodium perborate (as NaBO 3 •4H 2 O) 0-20% Bleach activator (TAED or NOBS) 0-5%
  • Enzymes (calculated as pure enzyme protein) 0.0001-0.1% Minor ingredients (e.g., perfume, optical brighteners) 0-3% 13) Detergent formulations as
  • the manganese catalyst may, e., be one of the compounds described in “Efficient manganese catalysts for low-temperature bleaching”, Nature 369, 1994, pp. 637-639.
  • the detergent may also comprise anionic surfactant and/or a bleach system.
  • the endoglucanase may be incorporated in concentrations conventionally employed in detergents. It is at present contemplated that, in the laundry composition of the invention, the cellulase may be added in an amount corresponding to 0.0001-10 mg (calculated as pure enzyme protein) of cellulase per liter of wash liquor.
  • endoglucanase may typically be a component of a fabric conditioning or softener composition.
  • a fabric conditioning or softener composition examples include, e.g., EP 0 233 910.
  • the present invention relates to use of the endoglucanase of the invention in the biopolishing process.
  • Bio-Polishing is a specific treatment of the yarn surface which improves fabric quality with respect to handle and appearance without loss of fabric wettability. The most important effects of Bio-Polishing can be characterized by less fuzz and pilling, increased gloss/luster, improved fabric handle, increased durable softness and altered water absorbency.
  • Bio-Polishing usually takes place in the wet processing of the manufacture of knitted and woven fabrics. Wet processing comprises such steps as, e.g., desizing, scouring, bleaching, washing, dying/printing and finishing. During each of these steps, the fabric is more or less subjected to mechanical action.
  • Desizing is the act of removing size from textiles.
  • warp yarns Prior to weaving on mechanical looms, warp yarns are often coated with size starch or starch derivatives in order to increase their tensile strength. After weaving, the size coating must be removed before further processing the fabric in order to ensure a homogeneous and wash-proof result. It is known that in order to achieve the effects of Bio-Polishing, a combination of cellulytic and mechanical action is required. It is also known that “super-softness” is achievable when the treatment with a cellulase is combined with a conventional treatment with softening agents.
  • Bio-polishing may be obtained by applying the method described, e.g., in WO 93/20278.
  • endoglucanase of the present invention may be applied advantageously, e.g., as follows:
  • the treatment of lignocellulosic pulp may, e.g., be performed as described in WO 91/14819, WO 91/14822, WO 92/17573 and WO 92/18688.
  • the present invention relates to use of the endoglucanase and/or enzyme preparation according to the invention for degradation of plant material, eg., cell walls.
  • Endoglucanases according to the invention may also be applied for enzymatic hydrolysis of various plant cell-wall derived materials or waste materials, e.g., agricultural residues such as wheat-straw, corn cobs, whole corn plants, nut shells, grass, vegetable hulls, bean hulls, spent grains, sugar beet pulp, and the like.
  • agricultural residues such as wheat-straw, corn cobs, whole corn plants, nut shells, grass, vegetable hulls, bean hulls, spent grains, sugar beet pulp, and the like.
  • the plant material may be degraded in order to improve different kinds of processing, facilitate purification or extraction of other components tike purification of beta-glucan or beta-glucan oligomers from cereals, improve the feed value, decrease the water binding capacity, improve the degradability in waste water plants, improve the conversion of, eg., grass and corn to ensilage, etc.
  • Isolate CBS 117.65, CBS 478.94, NRRL 8126, and ATCC 10523 were grown in shake flask cultures on cellulose enriched potato dextrose broth, incubated for 5 days at 26° C. (shaking conditions, 150 rpm).
  • mRNA was isolated from Myceliophthora thermophila, Acremonium sp., Thielavia terrestris and Volutella colletotrichoides , respectively, grown in a cellulose-containing fermentation medium with agitation to ensure sufficient aeration. Mycelia were harvested after 3-5 days' growth, immediately frozen in liquid nitrogen and stored at ⁇ 80° C. Libraries from Myceliophthora thermophila, Acremonium sp., Thielavia terrestris and Volutella colletotrichoides , respectively, each consisting of approx. 106 individual clones were constructed in E. coli as described with a vector background of 1%.
  • Plasmid DNA from some of the pools from each library was transformed into yeast, and 50-100 plates containing 250-400 yeast colonies were obtained from each pool.
  • Endoglucanase-positive colonies were identified and isolated on SC-agar plates with the AZCL HE cellulose assay. cDNA inserts were amplified directly from the yeast colonies and characterized as described in the Materials and Methods section above.
  • the DNA sequence of the cDNA encoding the endoglucanase from Myceliophthora thermophila is SEQ ID NO: 1 and the corresponding amino acid sequence is SEQ ID NO: 2.
  • the cDNA is obtainable from the plasmid in DSM 9770.
  • the DNA sequence of the cDNA encoding the endoglucanase from Acremonium sp. is SEQ ID NO: 7 and the corresponding amino acid sequence is SEQ ID NO: 8.
  • the cDNA is obtainable from the plasmid in DSM 10032.
  • the DNA sequence of the cDNA encoding the endoglucanase from Thielavia terrestris is SEQ ID NO: 11 and the corresponding amino acid sequence is SEQ ID NO: 12.
  • the cDNA is obtainable from the plasmid in DSM 10081.
  • the DNA sequence of the cDNA encoding the endoglucanase from Volutella colletotrichoides is SEQ ID NO: 21 and the corresponding amino acid sequence is SEQ ID NO: 22.
  • the cDNA is obtainable from the plasmid in DSM 10571.
  • Total DNA was isolated from a yeast colony and plasmid DNA was rescued by transformation of E. coli as described above.
  • the DNA was digested with appropriate restriction enzymes, size fractionated on gel, and a fragment corresponding to the endoglucanase gene from Myceliophthora thermophila, Acremonium sp., Thielavia terrestris and Volutella colletotrichoides , respectively, was purified.
  • the genes were subsequently ligated to pHD414, digested with appropriate restriction enzymes, resulting in the plasmids pA2C193, pA2C357, pA2C385 and pA2C488, respectively.
  • Each of the transformants were tested for endoglucanase activity as described above. Some of the transformants had endoglucanase activity which was significantly larger than the Aspergillus oryzae background. This demonstrates efficient expression of the endoglucanases in Aspergillus oryzae .
  • the transformants with the highest endoglucanase activity were selected and inoculated in a 500 ml shake flask with YPM media. After 3-5 days of fermentation with sufficient agitation to ensure good aeration, the culture broth was centrifuged for 10 minutes at 2000 g and the supernatant recovered.
  • the cellulytic activity of the endoglucanase may be determined relative to an analytical standard and expressed in the unit S-CEVU.
  • Cellulytic enzymes hydrolyze OMC, thereby decreasing the viscosity of the incubation mixture.
  • the resulting reduction in viscosity may be determined by a vibration viscometer (e.g., MIVI 3000 from Sofraser, France).
  • Determination of the cellulytic activity may be determined according to the analysis method AF 301.1 which is available from the Applicant upon request.
  • the S-CEVU assay quantifies the amount of catalytic activity present in the sample by measuring the ability of the sample to reduce the viscosity of a solution of carboxymethylcellulose (CMC).
  • the assay is carried out at 40° C., pH 7.5 using a relative enzyme standard for reducing the viscosity of the CMC substrate.
  • 1 SAVI-U is the amount of enzyme which forms an amount of reducing carbohydrates equivalent to 1 micro-mol of glucose per minute.
  • the reducing groups assay was performed according to Lever, M. A new reaction for colormetric determination of carbohydrates. Anal. Biochem. 1972. Vol 47 (273-279). Reagent mixture was prepared by mixing 1.5 gram p-hydroxybenzoic-acid hydrazide (PHBAH) with 5 gram sodium tartrate in 100 ml 2% sodium hydroxide.
  • PBAH p-hydroxybenzoic-acid hydrazide
  • PASC stock solution was prepared the following way using ice cold acetone and phosphoric acid. 5 gram of cellulose (Avicel®) was moistened with water, and 150 ml ice cold 85% ortho-phosphoric acid was added. The mixture was placed in ice bath under slow stirring for 1 hr. Then 100 ml ice cold acetone was added with stirring. The slurry was transferred to a Buchner filter with pyrex sintered disc number 3 and then washed three times with 100 ml ice cold acetone, and sucked as dry as possible after each wash.
  • the filter cake was washed twice with 500 ml water, sucked as dry as possible after each wash
  • the PASC was mixed with deionized water to a total volume of 300 ml, blended to homogeneity (using the Ultra Turrax Homogenizer) and stored in refrigerator (up to one month).
  • Substrate equilibration with buffer 20 gram phosphoric acid swollen cellulose PASC stock solution was centrifuged for 20 min at 5000 rpm., the supernatant was poured of; the sediment was resuspended in 30 ml of buffer and centrifuged for 20 min. at 5000 rpm., the supernatant was poured of, and the sediment was resuspended in buffer to a total of 60 g corresponding to a substrate concentration of 5 g cellulose/liter.
  • the enzyme solution is diluted in the same buffer as the substrate.
  • the substrate in buffer solution is preheated for 5 min. at 40° C. (2 ml). Then the enzyme solution (diluted to between 0.2 and 1 S-CEVU/ml) 0.5 ml is added and mixed for 5 sec. Enzymes blanks are obtained by adding the stop reagent before enzyme solution. Incubate for 20 min. at 40° C., The reaction is stopped by adding 0.5 ml 2% NaOH solution and mixing for 5 sec.
  • the samples are centrifuged for 20 min. at 5000 rpm. 1 ml supernatant is mixed with 0,5 ml PHBAH reagent and boiled for 10 min.
  • the test tubes are cooled in an ice water bath.
  • the absorbency at 410 nm is measured using a spectrophotometer. Blanks are prepared by adding sodium hydroxide before adding enzyme solution.
  • a standard glucose curve was obtained by using glucose concentrations of 5, 10, 15 and 25 mg/l in the same buffer and adding PHBAH reagent before boiling. The release of reducing glucose equivalent is calculated using this standard curve.
  • Aspergillus oryzae transformed with pA2C193 was grown on YPM medium for 4 days. The liquid was then centrifuged and sterile filtered.
  • the sample was concentrated by ultrafiltration on AMICON cells using a DOW membrane GR61PP with cut-off 20 kD.
  • the Uf-concentrate was analyzed for S-CEVU/ml and SaviU/ml with the following result:
  • the Extinction coefficient is based on the content of tyrosine, tryptophane and cysteine calculated from the sequence of SEQ ID NO: 2 (the amino acid sequence).
  • SOS-Page was performed on NOVEX Pre-Cast Gels 4-20% Tris-Glycine Gel 1.0 mm ⁇ 10 Well.
  • IEF was performed on Pharmacia PAGplate pH 3.5-9.5, the activity was visualized by CMC-Congored overlaying.
  • k m and k cat was determined in the same manner as the determination of SAVI Units at pH 8.5 with a substrate concentration up to 8 g/l.
  • the pH profile was determined at the following conditions:
  • Buffers of pH values between 2.5 and 10.0 were made by mixing 0.1 M Tri-sodium phosphate with 0.1 M citric acid. Purified endoglucanase was diluted to ensure the assay response to be within the linear range of the assay.
  • the substrate was a 0.4% suspension of AZCL-HE-cellulose (MegaZyme) mixed 1:1 with the citrate/phosphate buffer to a final substrate concentration of 0.2% AZCL-H E-cellulose.
  • 1 ml substrate in Eppendorf® 1.5 ml polypropylene tubes were added 10 microliters of enzyme solution and incubated for 15 minutes in Eppendorf® temperature controlled Thermomixers before heat-inactivation of enzymes for 20 minutes at 95° C. in a separate Thermomixer.
  • the tubes were centrifuged and 200 microliters of each supernatant was transferred to a well in a 96 well microtiter plate and OD was measured at 620 nm in an ELISA reader (Labsystems Multiskan® MCC/1340).
  • endoglucanase has more than 60% activity between pH 4.0 and 8.0 and optima activity at pH 5.0-6.0.
  • the temperature optimum was determined in the same manner at pH 5.5. The temperatures ranged from 30° C. to 80° C. For each temperature three incubations were carried out and the mean calculated. Three blanks were produced by immediate heat-inactivation of enzyme and the mean was subtracted from the incubated sample values.
  • endoglucanase has optimal activity at 50-70° C.
  • the temperature stability was determined in the same manner at pH 5.5 and 30° C., and, further, the enzyme solutions were preheated for 1 hour at the actual temperature and cooled on ice.
  • the residual activity is shown below in % of the activity of a non-preheated enzyme sample:
  • Apparatus Terg-o-tometer Liquid volume 100 ml Agitation 150 movements/min with vertical stirrer Rinse time 5 min in tapwater Washing temp 40° C. Washing liqour 0.05 M phosphate buffer pH 7.0 Washing time 30 min Repetitions 2 Enzymes Myceliophthora cellulase (SEQ ID NO: 2) Dosage 500 and 2500 S-CEVU/l Textile 2 swatches of aged black 100% cotton 5 ⁇ 6 cm (0.9 gram) Drying Tumble dry Evaluation The light remission is measured by a Datacolor Elrepho Remission spectrophotometer. Remission is calculated as delta L (Hunter Lab-values). When the surface fibrils and fibers protruding from the yarn are removed by the cellulase, the surface of the black fabric appears darker, and lower L values are obtained.
  • the sample is compared with a blind sample, i.e., washed without enzyme:
  • the purpose of the two constructions was to make derivatives of the endoglucanase from M. thermophila with the linker and CBD from the 43 kD endoglucanase from H. insolens (disclosed in WO 91/17243).
  • the native endoglucanase from M. thermophila do not have a linker and/or a cellulose binding domain, CBD.
  • CM1 Construction 1 consists of the endoglucanase from M. thermophila (225 amino acids) and the 72 C-terminal amino acids from the H. insolens 43 kD endoglucanase.
  • CM2 Construction 2 consists of the endoglucanase from M. thermophila (225 amino acids) and the 83 C-terminal amino acids from the H. insolens 43 kD endoglucanase.
  • the 43 kD endoglucanase cDNA from H. insolens was cloned into pHD414 in such a way that the endoglucanase gene was transcribed from the Taka-promoter.
  • the resulting plasmid was named pCaHj418.
  • primer 1 (SEQ ID NO: 88) 5′-CGGAGCTCACGTCCAAGAGCGGCTGCTCCCGTCCCTCCAGCAGCACC AGCTCTCCGG-3′ primer 2: (SEQ ID NO: 89) 5′-CCGGAGAGCTGGTGCTGCTGGAGGGACGGGAGCAGCCGCTCTTGGAC GTGAGCTCCG-3′ primer 3: (SEQ ID NO: 90) 5′-CGGAGCTCACGTCCAAGAGCGGCTGCTCCCGTAACGACGACGGCAAC TTCCCTGCCG-3′ primer 4 (SEQ ID NO: 91) 5′-CGGCAGGGAAGTTGCCGTCGTCGTTACGGGAGCAGCCGCTCTTGGAC GTGAGCTCCG-3′ Take-pro primer: (SEQ ID NO: 92) 5′-CAACATCACATCAAGCTCTCC-3′ AMS-term primer: (SEQ ID NO: 93) 5′-CCCCATCCTTTAACTATAGCG-3′
  • the endoglucanase fusions were constructed by the PCR overlap-extension method as described by Higuchi et al. 1988.
  • Reaction A The Polymerase Chain Reaction (PCR) was used to amplify the fragment of pCaHj418 between primer 1 and AMG-term. primer (the linker and CBD from the 43 kD endoglucanase from H. insolens ).
  • Reaction B PCR amplification of the fragment between Taka-pro, primer and primer 2 in pA2C193, the endoglucanase gene from M. thermophila.
  • Reaction C The two purified fragments were used in a third PCR in the presence of the primers flanking the total region, i.e., Taka-pro. primer and AMG-term. primer.
  • primer 3 and primer 4 had replaced respectively primer 1 and primer 2.
  • the fragment amplified in reaction C was purified, digested with restriction enzymes Xba I and BsstE II.
  • the purified digested fragment was ligated into pA2C193 digested with restriction enzymes Xba I and BsstE II.
  • Competent cells from E. coli strain DH5 ⁇ F′ (New England Biolabs.) were transformed with the ligated plasmid and colonies containing the gene fusion were isolated. The sequence of the cloned part was verified by DNA sequencing.
  • sequences of the genes in the two constructs are SEQ ID NO: 3 and SEQ ID NO: 5.
  • Reactions A and B started with 2 min. at 94° C. followed by 20 cycles of (30 sec. at 94° C., 30 sec. at 50° C. and 1 min. at 72° C.) and end with 4 min. at 72° C.
  • Reaction C started with (2 min. at 94° C., 1 min. at 52° C. and 2 min. at 72° C.), followed by 15 cycles of (30 sec. at 94° C., 30 sec. at 52° C. and 90 sec. at 72° C.) and end with 4 min. at 72° C.
  • the two constructs were transformed into Aspergillus oryzae as described above.
  • the cloned product is recovered after fermentation by separation of the extracellular fluid to from the production organism.
  • the Avicel is mixed with the crude fermentation broth, which contains total about 1 gram of cellulase. After mixing at 4° C. for 20 min the Avicel enzyme is packed into a column with a dimension of 50 times 200 mm about 400 ml total.
  • the column is washed with the 200 ml buffer, then washed with 0.5 M NaCl in the same buffer until no more protein elutes, Then washed with 500 ml 20 mm Tris pH 8.5. Finally the pure full length enzyme is eluted with 1% triethylamine pH 11.8.
  • the eluted enzyme solution is adjusted to pH 8 and concentrated using a Amicon cell unit with a membrane DOW GR61PP (polypropylene with a cut off of 20 KD) to above 5 mg protein per ml.
  • a membrane DOW GR61PP polypropylene with a cut off of 20 KD
  • the purified cellulases were characterized as follows;
  • the purified cellulases was analysed for MW by SDS-PAGE and using standard LMW protein marker kit from Pharmacia the MW was calculated for the cellulases.
  • the MW is apparently higher than the MW of the composition of the coding amino acids and is due to the fact the linker region is O-glycosylated resulting in this higher MW.
  • the pt was determined using a Pharmacia Ampholine PAG plate pH 3.5 to 9.5 and again using a Pharmacia kit with known pl proteins.
  • the molar extinction coefficient was calculated based on the amin acids composition using the known absorbance of Tryptophan, Tyrosine and Cystein.
  • pH activity profile was obtained using CMC substrate, incubation for 20 min at 40° C. at a 0.5 pH interval and measuring the formation of reducing sugars.
  • the relative activity at the different pH was calculated and the table contains the intervat with more than 50% relative activity has been measured.
  • the N-terminal was determined for the purified cellulase using a Applied Biosystems model 473A sequencer. The protein sequencer was run according to the manufacturer's instructions.
  • DSC Differential scanning calometry
  • the cellulases from Myceliophthora, Acremonium and Thielavia were used for raising antibody in rabbits. 0.1 mg of the purified cellulases in 0.9% NaCl solution mixed with Freunds adjuvant immediately prior to injection. The rabbits were immunized 10 times with one week interval.
  • the immunoglobulin G fraction (IgG) was purified by ammonium sulfate precipitation (25% saturation). The precipitate was solubilized in water and then dialyzed extensively against sodium acetate buffer (pH 5.0, 50 mM) altering with deionized water. After filtration, the IgG fraction was stabilized with sodium azide (0.01%).
  • Apparatus Terg-o-tometer Liquid volume 100 ml Agitation 150 movements/min (rpm) Rinse time 5 min in tap water Washing temp 40° C. Water Hardness 1 mM CaCl 2 Washing liquor 0.05 M phosphate buffer pH 7.0 Washing time 30 min Repetitions 2 Textile 2 swatches of aged black, 100% cotton 5 ⁇ 6 cm Drying Tumble dry
  • the light remission was measured by a Macbeth Color Eye 7000 Remission spectrophotometer. Remission is calculated as delta L (Hunter Lab-values). When the surface fibrils and fibers protruding from the yarn were removed by the cellulase, the surface appeared more bright and lower L values were obtained.
  • the light remission was measured by a Datacolor Elrepho Remission spectrophotometer. Remission is calculated as delta L (Hunter Lab-values). When the surface fibrils and fibers protruding from the yarn are removed by the cellulase, the surface of the black fabric appears darker and nicer, and lower L values are obtained.
  • Apparatus Terg-o-tometer Liquid volume 100 ml Agitation 150 movements/min with vertical stirrer Rinse time 5 min in tapwater Washing temp 40° Washing liqour 0.05 M phosphate buffer pH 7.0 Washing time 30 min Repetitions 2 Dosage 2.5 S-CEVU/ml Textile 2 swatches of aged black 100% cotton 5 ⁇ 6 cm (0.9 gram) Drying Tumble dry
  • the light remission is measured by a Datacolor Elrepho Remission spectrophotometer. Remission is calculated as delta L (Hunter Lab-values). When the surface fibrils and fibers protruding from the yarn are removed by the cellulase, the surface of the black fabric appears darker, and lower L values are obtained.
  • the sample is compared with a blind sample, i.e., washed without enzyme:
  • the light remission is measured by a Datacolor Elrepho Remission spectrophotometer. Remission is calculated as delta L (Hunter Lab-values). When the surface fibrils and fibers protruding from the yarn are removed by the cellulase, the surface of the black fabric appears darker and nicer, and lower L values are obtained.
  • Different dosages of cloned cellulases from Thielavia terrestris (SEQ ID NO: 12) and Acremonium sp. CBS 478.94 (SEQ ID NO: 8), respectively, (denoted A and B, respectively) were tested.
  • Apparatus Terg-o-tometer Liquid volume 150 ml Agitation 150 movements/min with vertical stirrer Rinse time 10 min in tapwater Washing temp 35° C. Hardness 1.0 mM CaCl 2 0.34 mM MgCl 2 Washing liqour 2.0 g/l HDL (neutral, citrate built HDL, with nonionic/anionic weight ration >0.5) pH 7.5 Washing time 30 min Repetitions 2 Textile 2 swatches of aged black cotton 5 ⁇ 6 cm (app. 150 g/m 2 ) 2 swatches of heavy knitted cotton 4 ⁇ 7 cm (app. 600 g/m 2 ) Drying Tumble dry
  • the light remission is measured by a Datacolor Elrepho Remission spectrophotometer. Remission is calculated as delta L (CIE Lab-values).
  • CIE Lab-values CIE Lab-values
  • the surface fibrils and fibers protruding from the yarn are removed by the cellulase, the surface of the black fabric appears darker and nicer, and lower L values are obtained.
  • Apparatus Washing machine Wascator FL 120 Liquid volume: 20 L Fabric: 1.1 kg denim fabric, 141 ⁇ 2 oz 100% cotton Desizing: 10 min, 55° C., pH 7 50 ml Aquazyme 120 L 2.5 g/l Phosphate buffer Abrasion: 2 hours;
  • Abrasion The remission from the fabric was determined at 420 nm using a Texflash 2000 as a measure of the abrasion level.
  • Abrasion Enzyme Dosage Trial conditions 420 nm Blank 0 S-CEVU/g textile pH 6, 55° C. 9.96 SEQ ID NO: 3 10 S-CEVU/g textile pH 6, 55° C. 14.37 Blank 0 S-CEVU/g textile pH 5, 65° C. 9.26 SEQ ID NO: 12 10 S-CEVU/g textile pH 5, 65° C. 16.86 Blank 0 S-CEVU/g textile pH 7, 45° C. 9.47 SEQ ID NO: 8 10 S-CEVU/g textile pH 7, 45° C. 14.08
  • Lyocell fibers which are sold under the trade name Tencel are spun from wood pulp its cellulose in a more environmentally friendly waterbased solvent than is the case for normal viscose production). How ever, the fibers have a tendency to fibrillate when they are processed into textiles which is seen on the surface and denoted “fuzz”.
  • fuzzy By using cellulases it is possible to permanently remove the exposed and Fuzzy fibers and significantly improve the look of the finished fabric, the treatment generally known as Biopolishing.
  • the endoglucanases of the present invention are especially suited for the removal of Lyocell surface fibers,
  • the textile substrate was either 100% woven or different kinds of jersey knitted dark blue almost.
  • the dark colour and jersey knit was preferred in order to enhance the visual effects which simplified the evaluation.
  • a woven 70/30 Tencel/Rayon blend was also used to a lesser extent.
  • the assays were either performed in 200 ml scale using a Launder-o-meter or in the 20 l scale using a Wascator.
  • the treatment time was 60 min at 55° C. in Wascator and 60-90 min in LOM.
  • the buffer was 2 g/l sodium acetate adjusted to pH 5 with acetic acid,
  • the fabric to liquid ratio was 1:10 but in the Launder-o-meter 20 steel balls with a diameter of 14 mm (11 g each) was used to obtain sufficient mechanical abrasion.
  • the biopolishing was immediately followed by inactivation using 2 g/l sodium carbonate at 80° C. for 15 min followed by rinsing in cold water.
  • the two cellulases are able to defibrillate both Tencel and Tencel blended fabrics.
  • an endoglucanase of the invention only small fibrils are removed rather than whole fibers such as is the case when using acid cellulase mixtures from Trichoderma .
  • the strength loss of the treated fabric is therefore kept at a minimum when using endoglucanases of the present invention.
  • S-CEVU/g fabric of cellulase from Acremonium sp SEQ ID NO: 8
  • 10 S-CEVU/g fabric of cellulase from Thielavia terrestris SEQ ID NO: 12
  • a New Cellulytic Enzyme was by Expression Cloning as Well as by PCR Cloning Detected to be Produced by a Plant Pathogen, Isolated from Soy Bean Seeds and Identified as Macrophomina Phaseolina
  • Isolate CBS 281.96 was grown in shake flask cultures on cellulose enriched potato dextrose broth, incubated for 5 days at 260 C (shaking conditions: 150 rpm).
  • mRNA was isolated from Macrophomina phaseolina , grown in a cellulose-containing fermentation medium with agitation to ensure sufficient aeration. Mycelia were harvested after 3-5 days' growth, immediately frozen in liquid nitrogen and stored at ⁇ 80° C. A library from Macrophomina phaseolina , consisting of approx. 10 6 individual clones was constructed in E. coli as described with a vector background of 1%.
  • Plasmid DNA from some of the pools was transformed into yeast, and 50-100 plates containing 250-400 yeast colonies were obtained from each pool.
  • Endoglucanase-positive colonies were identified and isolated on SC-agar plates with the AZCL HE cellulose assay, cDNA inserts were amplified directly from the yeast colonies and characterized as described in the Materials and Methods section above.
  • the DNA sequence of the cDNA encoding the endoglucanase is SEQ ID NO: 13 and the corresponding amino acid sequence is SEQ ID NO: 14.
  • the cDNA is obtainable from the plasmid in DSM 10512.
  • Total DNA was isolated from a yeast colony and plasmid DNA was rescued by transformation of E. coli as described above.
  • the DNA was digested with appropriate restriction enzymes, size fractionated on gel, and a fragment corresponding to the endoglucanase gene was purified, The gene was subsequently ligated to pHD414, digested with appropriate restriction enzymes, resulting in the plasmid pA2C477.
  • the plasmid was transformed into Aspergillus oryzae as described above.
  • the positive cDNA clones were characterized by sequencing the ends of the cDNA inserts, and by determining the nucleotide sequence of the longest cDNA from both strands.
  • the DNA sequence of the cDNA encoding the endoglucanase is SEQ ID NO: 13 and the corresponding amino acid sequence is SEQ ID NO: 14.
  • One construction was prepared in order to make a derivative of the endoglucanase from M. phaseolina with the linker and CBD from the 43 kD endoglucanase from H. insolens (disclosed in WO 91/17243)
  • the native endoglucanase from M. phaseolina does not have a linker and/or a cellulose binding domain, CBD.
  • the construction consists of the endoglucanase from M. phaseolina (222 amino acids) and the 72 C-terminal amino acids from the H. insolens 43 kD endoglucanase (SEQ ID NO: 24).
  • the 43 kD endoglucanase cDNA from H. insolens is cloned into pHD414 in such a way that the endoglucanase gene is transcribed from the Taka-promoter.
  • the resulting plasmid is named pCaHj418.
  • the cDNA encoding the endoglucanase from M. phaseolina (SEQ ID NO: 13) is cloned into pYES2.0 as a BstX I/Not I fragment and the resulting plasmid is named pC1C477.
  • primer 1 (SEQ ID NO: 94) 5′-GGTCGCCCGGACTGGCTGTTCCCGTACCCCCTCCAGCAGCACCAGCT CTCCGG-3′
  • primer 2 (SEQ ID NO: 95) 5′-CCGGAGAGCTGGTGCTGCTGGAGGGGGTACGGGAACAGCCAGTCCGG GCGACC-3′ pYES2.0
  • F.HT primer (SEQ ID NO: 96) 5′-CGGACTACTAGCAGCTGTAATACG-3′
  • AMO-term Primer (SEQ ID NO: 93) 5′-CCCCATCCTTTAACTATAGCG-3′
  • the endoglucanase fusion is constructed by the PCR overlap-extension method as described by Higuchi et al. 1988.
  • Reaction A The Polymerase Chain Reaction (PCR) is used to amplify the fragment of pCaHj418 between primer 1 and AMG-term. primer (the linker and CBD from the 43 kD 2 endoglucanase from H. insolens ).
  • Reaction B PCR amplification of the fragment between pYES2.0 F.HT primer and primer 2 in pC1C477, the endoglucanase gene from M. phaseolina.
  • Reaction C The two purified fragments are used in a third PCR in the presence of the primers flanking the total region, i.e., pYES2.0 F.HT primer and AMG-term. primer.
  • the fragment amplified in reaction C is purified, digested with restriction enzymes, e.g. Xba I and BamHI.
  • the purified digested fragment is ligated into pHD414 digested with restriction enzymes, e.g., Xba I and BamH I.
  • Competent cells from E. coli strain DH5 ⁇ F′ (New England Biolabs) are transformed with the ligated plasmid and colonies containing the gene fusion are isolated. The sequence of the cloned to part was verified by DNA sequencing.
  • Reaction A and B start with 2 min. at 94° C. followed by 20 cycles of (30 sec. at 94° C., 30 sec. at 52° C. and 1 min. at 72° C.) and ends with 4 min. at 72° C.
  • Reaction C starts with (2 min. at 94° C., 1 min. at 52° C. and 2 ml. at 72° C.), followed by 20 cycles of (30 sec. at 94° C., 30 sec. at 52° C. and 90 sec. at 72° C.) and ends with 4 min. at 72° C.
  • the construct may be transformed into Aspergillus oryzae as described above.
  • mRNA was isolated from Acremonium sp., CBS 478.94, and Sordaria fimicola , ATCC 52644, respectively, grown in a cellulose-containing fermentation medium with agitation to ensure sufficient aeration. Mycelia were harvested after 3-5 days' growth, immediately frozen in liquid nitrogen and stored at ⁇ 80° C. Libraries from Acremonium sp., and Sordaria fimicola , respectively, each consisting of approx. 106 individual clones were constructed in E. coli as described with a vector background of 1%.
  • Plasmid DNA from some of the pools from each library was transformed into yeast, and 50-100 plates containing 250-400 yeast colonies were obtained from each pool.
  • Endoglucanase-positive colonies were identified and isolated on SC-agar plates with the AZCL HE cellulose assay. cDNA inserts were amplified directly from the yeast colonies and characterized as described in the Materials and Methods section above.
  • the DNA sequence of the cDNA encoding the endoglucanase from Acremonium sp. is SEQ ID NO: 9 and the corresponding amino acid sequence is SEQ ID NO: 10.
  • the cDNA is obtainable from the plasmid in DSM 10080.
  • the partial DNA sequence of the cDNA encoding the endoglucanase from Sordaria fimicola is SEQ ID NO-25 (Nucleotide sequence of the 5′-end of the cDNA) and the corresponding amino acid sequence is SEQ ID NO: 26.
  • the cDNA is obtainable from the plasmid in DSM 10576.
  • Total DNA was isolated from a yeast colony and plasmid DNA was rescued by transformation of E. coli as described above.
  • the DNA was digested with appropriate restriction enzymes, size fractionated on gel, and a fragment corresponding to the endoglucanase gene from Acremonium sp. and Sordaria fimicola , respectively, was purified.
  • the genes were subsequently ligated to pHD414, digested with appropriate restriction enzymes, resulting in the plasmids pA2C371 and pA2C502, respectively.
  • Isolate CBS 280.96 was grown in static flask cultures, holding wheat bran medium (per flask, 300 g wheat bran added 450 ml salt solution), incubated for 6 days at 26° C. After incubation the wheat bran was extracted with distilled water (300 ml per flask) and the extract tested for endoglucanase activity (0.1% AZCL-HE-Cellulose (megazyme) in 1% agarose (Litex agarose, Medinova). Activity was observed on the plates holding pH of 3.0, 7.0 and 9.5.
  • mRNA was isolated from Crinipellis scabella grown as describe above. Mycelia were harvested after 3-5 days' growth, immediately frozen in liquid nitrogen and stored at ⁇ 80° C. A library from Crinipellis scabella , consisting of approx. 10 6 individual clones was constructed in E. coli as described with a vector background of 1%.
  • the DNA sequence of the cDNA encoding the endoglucanase is SEQ ID NO: 15 and the corresponding amino acid sequence is SEQ ID NO: 16.
  • the cDNA is obtainable from the plasmid in DSM 10511.
  • Total DNA was isolated from a yeast colony and plasmid DNA was rescued by transformation of E. coli as described above.
  • the DNA was digested with appropriate restriction enzymes, size fractionated on gel, and a fragment corresponding to the endoglucanase gene was purified.
  • the gene was subsequently ligated to pHD414, digested with appropriate restriction enzymes, resulting in the plasmid pA2C475.
  • the plasmid was transformed into Aspergillus oryzae as described above.
  • the native endoglucanase from Crinipellis scabella neither has a linker nor a cellulose binding domain (CBD).
  • the full-length cDNA contains an ATG start codon upstream from the endoglucanase encoding open reading frame (ORF), presumably resulting in scrambled translation initiation upon heterologous expression of the cDNA, such as in the yeast Saccharomyces cerevisiae and the filamentous fungus Aspergillus oryzae .
  • Construction 1 consists of the cDNA encoding the 226-residue endoglucanase from C. scabella fused by PCR with the 3′-end cDNA of H. insolens coding for the linker and CBD region (72 amino acids) at the COOH-terminus of the H. insolens 43 kD endoglucanase.
  • the second hybrid construct is identical to the abovementioned gene fusion, except that the first five residues from the putative signal peptide have been deleted by PCR resulting in a shorter signal, which starts with the second in-frame ATG start codon.
  • the plasmid pC1C475 contains the full-length cDNA from C. scabella , cloned into BstXI/NotI-cut yeast expression vector pYES 2.0, the plasmid pC1C144 contains the full-length cDNA from H. insolens , cloned into the BstXI site of pYES 2.0.
  • Two PCR fragments encoding the core region of the endoglucanase from C. scabella were generated in PCR buffer (10 mM Tris-HCl, pH 8.3, 50 mM KCl, 1.5 mM MgCl 2 , 0.01% gelatin; containing 200 micro-M each dNTP), using 50-100 ng of pC1C475 as template, and 250 pmol of the reverse primer (5′-GACCGGAGAGCTGGTGTGCTGGAGGGTTTACGACACAGCCCGAGATATAGTG-3 (SEQ ID NO: 97)) in two combinations with 300-350 pmol of each forward primer (forward no.
  • insolens was generated in PCR buffer (10 mM Tris-HCl, pH 8.3, 50 mM KCl, 1.5 mM MgCl 2 , 0.01% gelatin: containing 200 micro-M each dNTP) using 100 ng of the pC1C144 template, 250 pmol forward primer (5′-CACTAATATCTCGGCTGTGTTCGTAACCCTCCAGCAGCACCAGCTCTCCGGTC-3′(SEQ ID NO:100)), 250 pmol of the pYES 2.0 reverse primer (5′-GGGCGTGAATGTAAGCGTGACATA-3′ (SEQ ID NO-101)), a DNA thermal cycler (Landgraf, Germany) and 2.5 units of Taq polymerase (Perkin-Elmer, USA).
  • PCR buffer 10 mM Tris-HCl, pH 8.3, 50 mM KCl, 1.5 mM MgCl 2 , 0.01% gelatin: containing 200 micro-M each dNTP
  • PCR products were electrophoresed in 0.7% low gelling temperature agarose gels (SeaPlaque, FMC), the fragments of interest were excised from the gel and recovered by treatment with agarase (New England Biolabs, USA) according to the manufacturer's instructions, followed by phenol extraction and ethanol precipitation at ⁇ 20° C. for 12 h by adding 2 vols of 96% EtOH and 0.1 vol of 3 M NaAc.
  • the recombinant hybrid genes between the endoglucanase from Crinipellis scabella and the linker/CBD region of the 43 kD endoglucanase from Humicola insolens were generated by combining the overlapping PCR fragments from above (ca 50 ng of each template) in two combinations in PCR buffer (10 mM Tris-HCl, pH 8.3, 50 mM KCl, 1.5 mM MgCl 2 , 0.01% gelatin; containing 200 micro-M each dNTP).
  • the SOE reaction was carried out using the DNA thermal cycler (Landgraf, Germany) and 2.5 units of Taq polymerase (Perkin-Elmer, Cetus, USA).
  • the PCR-generated, recombinant fragments were electrophoresed in a 0.7% low gelling temperature agarose gel (SeaPlaque, FMC), the fragments of interest were excised from the gel and recovered by treatment with agarase (New England Biolabs, USA) according to the manufacturer's instructions, followed by phenol extraction and ethanol precipitation at ⁇ 20° C. for 12 h.
  • the DNA fragments were digested to completion with HindIII and XbaI, and ligated into HindIII/XbaI-cleaved pHD414 vector followed by electroporation of the constructs into E. coli DH10B cells according to the manufacturer's instructions (Life Technologies, USA).
  • the nucleotide sequence of the resulting gene fusions was determined from both strands as described in the Materials and methods section, SEQ ID NOS: 17 and 19.
  • the constructs may be transformed into A. oryzae as described.
  • Rhizophlyctis rosea (de Bary & Wor) Fischer
  • Helicostylum fresenii Acc No of strain NRRL 2305 42 Trichothecium roseum , Acc No of strain: IFO 5372
  • Endophyte isolated from leaf of flowering plant
  • strains were maintained on agar in petrie dishes (9 cm) or on slants (see list of Media: PCA and PDA). 44 of the strains were grown in shake flasks under the following growth conditions: general fungal media as PC, PD and PB 9 or YPG (see list of media); incubation time from 3 to 9 days, temperature 26° C.; rpm between 150 and 175. Strain No 14 ( F. poae ) was grown on wheat bran for 15 days (26° C.; static) Strain No 38 was grown in dilute salt solution (DS/2), added 1 cm 2 pieces of autoclaved filter paper.
  • general fungal media as PC, PD and PB 9 or YPG (see list of media)
  • incubation time from 3 to 9 days, temperature 26° C.; rpm between 150 and 175.
  • Strain No 14 F. poae
  • Strain No 38 was grown in dilute salt solution (DS/2), added 1 cm 2 pieces of autoclaved filter paper.
  • the biomass was harvested by filtering (mesh adjusted to the growth of the fungus, the finest used for fungi which have highly sporulating mycelium as, e.g., Fusarium spp.)
  • the biomass on the filter was scraped into a sterile plastic bag and immediately frozen (by submerging into liquid nitrogen).
  • Thermomyces verrucosus CBS 285.96; SEQ ID NO: 29 (and the deduced amino acid sequence in SEQ ID NO: 30);
  • Nectria pinea CBS 279.96: SEQ ID NO: 35 (and the deduced amino acid sequence in SEQ ID NO:36);
  • Humicola grisea ATCC 22726: SEQ ID NO: 37 (and the deduced amino acid sequence in SEQ ID NO: 38);
  • NKBC 1515 SEQ ID NO: 43 (and the deduced amino acid sequence in SEQ ID NO: 44):
  • Chaetostylum fresenii SEQ ID NO: 47 (and the deduced amino acid sequence in SEQ ID NO: 48);
  • Exidia glandulosa CBS 277.96: SEQ ID NO: 49 (and the deduced amino acid sequence in SEQ ID NO: 50);
  • Coniothecium sp. SEQ ID NO: 51 (and the deduced amino acid sequence in SEQ ID NO: 52);
  • Diplodia gossypina CBS 274.96: SEQ ID NO: 57 (and the deduced amino acid sequence in SEQ ID NO: 58);
  • Ulospora bilgramii , NKBC 1444 SEQ ID NO: 59 (and the deduced amino acid sequence in SEQ ID NO: 60);
  • Penicillium verruculosum ATCC 62396: SEQ ID NO: 61 (and the deduced amino acid sequence in SEQ ID NO: 62);
  • Thielavia thermophila CBS 174.70: SEQ ID NO: 67 (and the deduced amino acid sequence in SEQ ID NO: 68);
  • Chaetomium virescens SEQ ID NO: 71 (and the deduced amino acid sequence in SEQ ID NO: 72);
  • Colletotrichum lagenarium SEQ ID NO: 73 (and the deduced amino acid sequence in SEQ ID NO: 74);
  • Trichothecium roseum SEQ ID NO: 77 (and the deduced amino acid sequence in SEQ ID NO: 78);
  • Color clarification is measured as removal of surface fibrils and fibers protruding from the yarn of a textile containing cellulosic fibers.
  • Apparatus Terg-o-tometer Liquid volume 100 ml Agitation 150 movements/min with vertical stirrer Rinse time 5 min in tapwater Washing temp 40° Washing liqour 0.05 M phosphate buffer pH 7.0 Washing time 30 min Repetitions 2 Enzymes Crude supernatants from the strains shown below.
  • the light remission is measured by a Datacolor Elrepho Remission spectrophotometer. Remission is calculated as delta L (Hunter Lab-values). When the surface fibrins and fibers protruding from the yarn are removed by the cellulase, the surface of the black fabric appears darker, and lower L values are obtained.
  • the samples are compared with a blind sample, i.e., washed without enzyme. Below is shown the delta L remission values compared to a blind sample.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Textile Engineering (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
  • Paper (AREA)
  • Treatments For Attaching Organic Compounds To Fibrous Goods (AREA)
  • Peptides Or Proteins (AREA)
  • Compounds Of Unknown Constitution (AREA)

Abstract

The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence
(SEQ ID NO: 79) Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa 1   2   3   4   5   6   7   8   9   10  11  12 Trp Xaa 13  14

and a second amino acid sequence having the following sequence
Trp Cys Cys Xaa Cys (SEQ ID NO: 80) 1   2   3   4   5

wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.

Description

    CROSS-REFERENCE TO RELATED APPLICATIONS
  • This application is a continuation of application Ser. No. 10/965,499 filed on Oct. 14, 2004, which is a continuation of U.S. application no. 10/007,521 filed Dec. 10, 2001, now U.S. Pat. No. 6,855,531, which is a continuation of U.S. application no. 09/229,911 filed Jan. 13, 1999, now U.S. Pat. No. 6,387,690, which is a divisional of U.S. application Ser. No. 08/651,136 filed May 21, 1996, now U.S. Pat. No. 6,001,639, which is a continuation of international application no. PCT/DK96/00105 filed Mar. 18, 1996, which claims priority under 35 U.S.C. 119 of Danish application nos. 0272/95, 0885/95, 0886/95, 0887/95, 0888/95, and 0137/96 filed Mar. 17, 1995, Aug. 8, 1995, Aug. 8, 1995, Aug. 8, 1995, Aug. 8, 1995 and Feb. 12, 1996, respectively, the contents of which are fully incorporated herein by reference.
    • FIELD OF THE INVENTION
  • The present invention relates to novel enzyme preparations comprising an enzyme exhibiting endoglucanase activity which performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.
    • BACKGROUND OF THE INVENTION
  • Cellulases or cellulytic enzymes are enzymes involved in hydrolysis of cellulose. In the hydrolysis of native cellulose, it is known that there are three major types of cellulase enzymes involved, namely cellobiohydrolase (1,4-beta-D-glucan cellobiohydrolase, EC 3.2.1.91), endo-beta-1,4-glucanase (endo-1,4-beta-D-glucan 4-glucanohydrolase, EC 3.2.1.4) and beta-glucosidase (EC 3.2.1.21).
  • Cellulases are synthesized by a large number of microorganisms which include fungi, to actinomycetes, mycobacteria and true bacteria but also by plants. Especially endoglucanases of a wide variety of specificities have been identified.
  • A very important industrial use of cellulytic enzymes is the use for treatment of cellulosic textile or fabric, e.g., as ingredients in detergent compositions or fabric softener compositions, for bio-polishing of new fabric (garment finishing), and for obtaining a “stone-washed” look of cellulose containing fabric, especially denim, and several methods for such treatment have been suggested, e.g., in GB-A-1 368 599, EP-A-0 307 564 and EP-A-0 435 876, WO 91/17243, WO 91/10732, WO 91/17244, PCT/DK95/000108 and PCT/DK95/00132.
  • Another important industrial use of cellulytic enzymes is the use for treatment of paper pulp, e.g., for improving the drainage or for deinking of recycled paper.
  • Especially the endoglucanases (EC No. 3.2.1.4) constitute an interesting group of hydrolases for the mentioned industrial uses. Endoglucanases catalyses endo hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (such as carboxy methyl cellulose and hydroxy ethyl cellulose), lichenin, beta-1,4 bonds in mixed beta-1,3 glucans such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. The authorized name is endo-1,4-beta-D-glucan 4-glucano hydrolase, but the abbreviated term endoglucanase is used in the present specification. Reference can be made to T.-M. Enveri, “Microbial Cellulases” in W. M. Fogarty, Microbial Enzymes and Biotechnology, Applied Science Publishers, p. 183-224 (1983); Methods in Enzymology, Vol. 160, p. 200-391 (1988) (edited by Wood, W. A. and Kellogg, S. T.); Béguin, P., “Molecular Biology of Cellulose Degradation”, Annu. Rev. Microbiol. Vol. 44, pp. 219-248 (1990); Béguin, P. and Aubert, J-P., “The biological degradation of cellulose”, FEMS Microbiology Reviews Vol. 13, pp. 25-58 (1994); Henrissat, B., “Cellulases and their interaction with cellulose”, Cellulose, Vol. 1, pp. 169-196 (1994).
  • Fungal endoglucanases have been described in numerous publications, especially those derived from species as, e.g., Fusarium oxysporum, Trichoderma reesei, Trichoderma longibrachiatum, Aspergillus aculeatus, Neocallimastix patriciarum, and, e.g., from species of the genera Piromyces, Humicola, Myceliophthora, Geotricum, Penicillium, Irpex, Coprinus.
  • For example, fungal endoglucanases have been described by Sheppard, P. O., et al., “The use of conserved cellulase family-specific sequences to clone Cellulase homologue cDNAs from Fusarium oxysporum, Gene, Vol. 15, pp. 163-167 (1994), Saloheimo, A., et al., “A novel, small endoglucanase gene, egl5, from Trichoderma reesei isolated by expression in yeast”, Molecular Microbiology Vol. 13(2), pp. 219-228 (1994); van Arsdell, J. N. et al., Cloning, characterization, and expression in Saccharomyces cerevisiae of endoglucanase I from Trichoderma reesei, Bio/Technology 5, 60-64 (1987); Penttilä, M. et al., “Homology between cellulase genes of to Trichoderma reesei: complete nucleotide sequence of the endoglucanase I gene”, Gene 45: 253-263 (1986), Saloheimo, M. et al, “EGIII, a new endoglucanase from Trichoderma reesei the characterization of both gene and enzyme”, Gene 63: 11-21 (1988), Gonzales, R., et al., “Cloning, sequence analysis and yeast expression of the egl1 gene from Trichoderma longibrachiatum”, Appl. Microbiol. Biotechnol., Vol. 38, pp. 370-375 (1992); Ooi, T. et al. “Cloning and sequence analysis of a cDNA for cellulase (FI-CMCase) from Aspergillus aculeatus”, Curr. Genet., Vol. 18, pp. 217-222 (1990); Ooi, T. et al, “Complete nucleotide sequence of a gene coding for Aspergillus aculeatus cellulase (FI-CMCase)”, Nucleic Acids Research, Vol. 18, No. 19, p. 5884 (1990); Xue, G. et al., “Cloning and expression of multiple cellulase cDNAs from the anaerobic rumen fungus Neocallimastix patriciarum in E. coli”, J. Gen. Microbiol., Vol. 138, pp 1413-1420 (1992); Xue, G. et al., “A novel polysaccharide hydrolase cDNA (celD) from Neocallimastix patriciarum encoding three multi-functional catalytical domains with high endoglucanase, cellobiohydrolase and xylanase activities”, 3. Gen. Microbiol., Vol. 138, pp. 2397-2403 (1992), Zhou, L. et al., “Intronless celB from the anaerobic fungus Neocallimastix patriciarum encodes a modular family A endoglucanase”, Biochem. J., Vol. 297, pp. 359-364 (1994); Dalbøge, H. and Heldt-Hansen. H. P. “A novel method for efficient expression cloning of fungal enzyme genes”, Mol. Gen. Genet., Vol. 243, pp. 253-260 (1994); Ali, B. R. S. et al., “Cellulases and hemicellulases of the anaerobic fungus Piromyces constitute a multiprotein cellulose-binding complex and are encoded by multigene families”, “FEMS Microbiol. Lett., Vol. 125, No. 1, pp. 15-21 (1995). Further, the DNA Data Bank of Japan (DDBJ database publicly available at Internet) comprises two DNA sequences cloned from Penicillium janthinellum encoding endoglucanases (cloned by A. Koch and G. Mernitz, respectively) and a DNA sequence cloned from Humicola grisea var thermoidea encoding an endoglucanase (cloned by T. Uozumi). Two endoglucanases from Macrophomina phaseolina have been cloned and sequenced, see Wang, H. Y. and Jones, R. W. “Cloning, characterization and functional expression of an endoglucanase-encoding gene from the phytopathogenic fungus Macrophomina phaseolina” in Gene, 158:125-128, 1995, and Wang, H. Y. and Jones, R. W.: “A unique endoglucanase-encoding gene cloned from the phytopathogenic fungus Macrophomina phaseolina” in Applied And Environmental Microbiology, 61:2004-2006, 1995. One of these endoglucanases shows high homology to the egl3 endoglucanase from the fungus Trichoderma reesei, the other shows homology to the egl1 from the microbial phytopathogen Pseudomonas solanacearum indicating that both endoglucanases belong to family 5 of glycosyl hydrolases (B. Henrissat, Biochem J 280; 309-316 (1991)). Filament-specific expression of a cellulase gene in the dimorphic fungus Ustilago maydis is disclosed in Schauwecker, F. et al. (1995).
  • WO 91/17243 (Novo Nordisk A/S) discloses a cellulase preparation consisting of a homogenous endoglucanase component immunoreactive with an antibody raised against a highly purified 43 kDa endoglucanase derived from Humicola insolens, DSM 1800; WO 91/17244 (Novo Nordisk A/S) discloses a new (hemi)cellulose degrading enzyme, such as an endoglucanase, a cellobiohydrolase or a beta-glucosidase, which may be derived from fungi other than Trichoderma and Phanerochaete; WO 93/20193 discloses an endoglucanase derivable from Aspergillus aculeatus; WO 94/21801 (Genencor Inc.) concerns a cellulase system isolated from Trichoderma longibrachiatum exhibiting endoglucanase activity, WO 94/26880 (Gist Brocades N. V.) discloses an isolated mixture of cellulose degrading enzymes, which preferable are obtained from Trichoderma Aspergillus or Disporotrichum, comprising endoglucanase, cellobiohydrolase, and xyloglucanase activity; and WO 95/02043 (Novo Nordisk A/S) describes an enzyme with endoglucanase activity derived from Trichoderma harzianum, which can be used for a number of purposes including e.g., degradation or modification of plant cell walls.
  • It is also known that cellulases may or may not have a cellulose binding domain (a CBD). The CBD enhances the binding of the enzyme to a cellulose containing fiber and increases the efficacy of the catalytic active part of the enzyme.
  • There is an ever existing need for providing novel cellulase enzyme preparations which may be used for applications where cellulase preferably an endoglucanase, activity is desirable.
  • The object of the present invention is to provide novel enzyme preparations having substantial cellulytic activity at acid, neutral or alkaline conditions and improved performance in paper pulp processing, textile treatment, laundry processes or in animal feed; preferably novel cellulases, more preferably well-performing endoglucanases, which are contemplated to be producible or produced by recombinant techniques.
    • SUMMARY OF THE INVENTION
  • Surprisingly, it has been found that a group of endoglucanases having certain unique characteristics perform very well in those industrial applications for which endoglucanases are conventionally used. These unique characteristics can be described in terms of conserved regions of the amino acid sequence of the enzyme protein and the inventors have found that cellulytic enzymes, i.e., enzymes exhibiting cellulytic activity, having certain conserved regions are very effective, e.g., in the treatment of laundry, in the treatment of newly manufactured textile, in the treatment of papermaking pulp.
  • Accordingly, in its first aspect the present invention relates to an enzyme preparation consisting essentially of an enzyme having cellulytic activity and comprising a first amino acid sequence consisting of 14 amino acid residues having the following sequence
  • (SEQ ID NO: 79)
    Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa
    1   2   3   4   5   6   7   8   9   10  11  12
    Trp Xaa
    13  14

    and a second amino acid sequence consisting of 5 amino acid residues having the following sequence
  • Trp Cys Cys Xaa Cys (SEQ ID NO: 80)
    1   2   3   4   5

    wherein
  • at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe;
  • at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe;
  • at position 8 of the first sequence, the amino acid is Arg, Lys or His;
  • at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala.
  • This surprising finding of clearly recognisable conserved regions, in spite of rather prominent variations found within well-performing endoglucanase enzymes, is a result of studies of a number of fungal DNA sequences encoding for specific amino acid sequences of enzymes having significant cellulytic, especially endoglucanase, activities.
  • Based on this finding, a novel molecular method tailored to screen specifically for genomic DNA or cDNA characterized by encoding the enzymes of the invention has been developed. As tools for these three sets of degenerated primers were constructed. Accordingly, in its second aspect, the invention relates to a method for providing a gene encoding for cellulytic enzymes having the above conserved regions.
  • By using this method, i.e., the set of primers for a PCR screening on genomic DNA, it was surprisingly found that DNA encoding for said enzymes can be found from a broad range of fungi, belonging to taxonomically very different organisms and inhabiting ecologically very different niches.
  • Further, by using this method it has been possible to find DNA sequences encoding for the core regions (catalytically active regions or domains) of said enzymes without any attached cellulose binding domain (CBD) which core regions of enzymes would not have been selected by using conventional performance based screening approaches. The inventors have verified experimentally that the linking of a CBD region to a core region enzyme (comprising the catalytically active region or domain of the enzyme) of the present invention results in a significantly improved performance, e.g., a fifty times higher performance., of the multiple domain enzyme.
  • Accordingly, the present invention provides novel cellulases, especially endoglucanases, having improved performance in industrial applications, either in their native form, or homo- or heterologously produced.
  • In further aspects, the present invention relates to novel cellulytic enzyme preparations which are derivable from taxonomically specific phyli, classes, orders, families, genera, and species; e.g., from Basidiomycotous Hymenomycetes, Zygomycota, Chytridiomycota; or from the classes Discomycetes, Loculoascomycetes, Plectomycetes; Archaeascomycetes, Hemiascomycetes or from the orders Diaportales, Xylariales, Trichosphaeriales, Phyllachorales; or from the families Nectriaeae, Sordariaceae, Chaetomiaceae, Ceratostomaceae, Lasiosphaeriaceae; or from the genera Cylindrocarpon, Gliocladium, Volutella, Scytalidium, Acremonium, or from the species Fusarium lycopersici, Fusarium passiflora, Fusarium solani, Fusarium anguioides, Fusarium poae, Humicola nigrescens, Humicola grisea, especially such consisting essentially of an enzyme comprising an amino acid sequence selected from the group consisting of the sequences
  • (SEQ ID NOS: 105-107)
    Xaa Thr Arg Xaa Phe Asp Xaa
    1   2   3   4   5   6   7;
    Xaa Thr Arg Xaa Tyr Asp Xaa
    1   2   3   4   5   6   7;
    and
    Xaa Thr Arg Xaa Trp Asp Xaa
    1   2   3   4   5   6   7

    wherein Xaa at position 4 is Trp, Tyr or Phe and Xaa at positions 1 and 7 is any of the 20 naturally occurring amino acid residues.
  • More specifically, the enzyme preparation of the invention is preferably obtainable from the taxonomically specific phyli, classes, orders, families, genera, and species mentioned above which all produce endoglucanases comprising a first peptide consisting of 13 amino acid residues having the following sequence
  • (SEQ ID NO: 108)
    Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa
    1   2   3   4   5   6   7   8   9   10  11  12
    Trp
    13

    and a second peptide consisting of 5 amino acid residues having the following sequence
  • Trp Cys Cys Xaa Cys (SEQ ID NO: 80)
    1   2   3   4   5

    wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, and 12, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues.
  • In yet further aspects, the present invention provides DNA constructs comprising a DNA sequence encoding an enzyme exhibiting endoglucanase activity, which DNA sequence comprises the DNA sequence of SEQ ID NOS: 1, 7, 9, 11, 13, 15, 21, and 25, respectively, or analogues thereof.
  • The present invention also relates to a recombinant expression vector comprising a DNA construct of the invention, to a cell comprising a DNA construct or a recombinant expression vector of the invention; to a method of producing an enzyme, e.g., a recombinant enzyme, of the invention; to a method of providing colour clarification of laundry by using the enzyme of the invention to a laundry composition comprising the enzyme of the invention, to uses of the enzyme of the invention for degradation or modification of plant material, e.g., cell walls, for treatment of fabric, textile or garment, for treatment of paper pulp; and to an enzyme preparation which is enriched in an enzyme of the present invention.
    • BRIEF DESCRIPTION OF THE FIGURES
  • FIGS. 1A, 1B and 1C show an alignment of the deduced encoded amino acid sequences of Acremonium sp, (I) (SEQ ID NO: 8), Volutella colletotrichoides (SEQ ID NO: 22), Crinipellis scabella (SEQ ID NO: 16), Acremonium sp. (II) (SEQ ID NO: 10), Myceliophthora thermophila (SEQ ID NO: 2), Thielavia terrestris (SEQ ID NO: 12), Macrophomina phaseolina (SEQ ID NO: 14). The Pileup program (Feng and Doolittle, 1987) (GCG package, version 8.0) was used to create the best alignment. Identical residues in at least four sequences (boxed) are indicated around the corresponding amino acids.
  • FIGS. 2A, B and C illustrate the taxonomic classification within the Fungal Kingdom of all the microorganisms disclosed herein as being capable of producing said enzyme preparations and enzymes of the invention.
    •
  • The taxonomic classification used herein builds primarily on the system used in the NIH Data Base (Entrez, version spring 1996) available on the internet.
  • Regarding classification of organisms which are not included in the Entrez data base the following generally available and world wide accepted reference books have been used:
  • For Ascomycetes: Eriksson, O. E. & Hawksworth, D. L.: Systema Ascomycetum vol 12 (1993).
  • For Basidiomycetes: Jülich, W.: Higher Taxa of Basidiomycetes, Bibliotheca Mycologia 85, 485 pp (1981).
  • For Zygomycetes: O'Donnell, K.: Zygomycetes in culture, University of Georgia, US, 257 pp (1979).
  • General Mycological Reference Books;
  • Hawksworth, D. L., Kirk, P. M., Sutton, B. C. and Pegler, D. N.: Dictionary of the fungi, International Mycological Institute, 616 pp (1995);
    Von Arx, J. A.: The genera of fungi sporulating in culture, 424 pp (1981),
  • The taxonomic implacement of the genus Humicola has until recently remained unclear. However, studies of 18SRNA of a wide selection of Sordariales has given strong indications of referring Humicola to the order Sordariales (Taylor, Clausen & Oxenbøll, unpublished). Further these data suggests Humicola along with Scytalidium to be only rather distantly related to the families Sordariaceae, Chaetomiaceae, Ceratostomataceae, and Lasiosphaeriaceae. In accordance with the above Humicola and Scytalidium are here placed within the order Sordariales, with unclassified Family.
  • FIGS. 3A and 38 show an alignment of the deduced partial amino acid sequences derived from a selection of 26 of the 46 microorganisms described in Example 5 (SEQ ID NOS: 40, 30, 38, 74, 64, 32, 52, 62, 66, 28, 34, 68, 76, 72, 46, 54, 42, 36, 48, 44, 78, 58, 50, 60, and 56).
    • DETAILED DESCRIPTION OF THE INVENTION
  • In the present context, the term “the 20 naturally occurring amino acid residues” denotes the amino acid residues usually found in proteins and conventionally known as alanine (Ala or A), valine (Val or V), leucine (Leu or L), isoleucine (Ile or I), proline (Pro or P), phenylalanine (Phe or F), tryptophan (Trp or W), methionine (Met or M), glycine (Gly or G), serine (Ser or S), threonine (Thr or T), cysteine (Cys or C), tyrosine (Tyr or Y), asparagine (Asn or N), glutamine (Gln or Q), aspartic acid (Asp or D), glutamic acid (Glu or E), lysine (Lys or K), arginine (Arg or R), and histidine (H is or H).
  • According to the present invention there is provided novel well-performing endoglucanases comprising conserved amino acid sequence regions, especially a first amino acid sequence consisting of 14 amino acid residues having the following sequence
  • (SEQ ID NO: 79)
    Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa
    1   2   3   4   5   6   7   8   9   10  11  12
    Trp Xaa
    13  14

    and a second amino acid sequence consisting of 5 amino acid residues having the following sequence
  • Trp Cys Cys Xaa Cys (SEQ ID NO: 80)
    1   2   3   4   5

    wherein
  • at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe;
  • at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe;
  • at position 8 of the first sequence, the amino acid is Arg, Lys or His;
  • at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala.
  • Preferably, the enzyme of the invention is of microbial origin, i.e., obtainable from a microorganism such as a fungus.
  • In a preferred embodiment, the amino acid residue at position 9 of the first sequence is selected from the group consisting of proline, threonine, valine, alanine, leucine, isoleucine, phenylalanine, glycine, cysteine, asparagine, glutamine, tyrosine, serine, methionine and tryptophan, preferably from the group consisting of proline and threonine.
  • In another preferred embodiment, the amino acid residue at position 10 of the first sequence is selected from the group consisting of proline, threonine, valine, alanine, leucine, isoleucine, phenylalanine, glycine, cysteine, asparagine, glutamine, tyrosine, serine, methionine and tryptophan, preferably serine.
  • In yet another preferred embodiment, the amino acid residue at position 12 of the first sequence is selected from the group consisting of proline, threonine, valine, alanine, leucine, isoleucine, phenylalanine, glycine, cysteine, asparagine, glutamine, tyrosine, serine, methionine and tryptophan, preferably from the group consisting of alanine and glycine.
  • In yet another preferred embodiment, the amino acid residue at position 14 of the first sequence is selected from the group consisting of proline, threonine, valine, alanine, leucine, isoleucine, phenylalanine, glycine, cysteine, asparagine, glutamine, tyrosine, serine, methionine, tryptophan, glutamic acid and aspartic acid, preferably from the group consisting of proline, threonine, serine, alanine, glutamic acid and aspartic acid.
  • Preferably, the amino acid residue at position 4 of the second sequence is selected from the to group consisting of proline, threonine, valine, alanine, leucine, isoleucine, phenylalanine, glycine., cysteine, asparagine, glutamine, tyrosine, serine, methionine, tryptophan, glutamic acid and aspartic acid, more preferably from the group consisting of alanine, glycine, and glutamine.
  • Examples of more preferred embodiments are such wherein, in the first sequence, the amino acid residue at position 3 is tyrosine; or the amino acid residue at position 4 is tryptophan; or the amino acid residue at position 8 is lysine.
  • In an especially preferred embodiment, the enzyme of the invention has a first sequence comprising the amino acid sequence
  • (SEQ ID NO: 79)
    Thr Arg Tyr Trp Asp Cys Cys Lys Pro Ser Cys Ala
    1   2   3   4   5   6   7   8   9   10  11  12
    Trp
    13,

    or the amino acid sequence
  • (SEQ ID NO: 79)
    Thr Arg Tyr Trp Asp Cys Cys Lys Thr Ser Cys Ala
    1   2   3   4   5   6   7   8   9   10  11  12
    Trp
    13,

    or the amino acid sequence
  • (SEQ ID NO: 79)
    Thr Arg Tyr Trp Asp Cys Cys Lys Pro Ser Cys Gly
    1   2   3   4   5   6   7   8   9   10  11  12
    Trp
    13.
  • In a second aspect, the present invention provides a method for providing a microbial strain comprising a gene encoding such an enzyme which method comprises hybridization, e.g., PCR amplification, under standard conditions with an oligonucleotide derived from any of the conserved regions, illustrated in FIG. 1.
  • A useful oligonucleotide comprises a nucleotide sequence encoding at least a pentapeptide comprised in a peptide selected from the group consisting of
  • (SEQ ID NO: 79)
    Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa
    1   2   3   4   5   6   7   8   9   10  11  12
    Trp Xaa
    13  14

    the amino acid at position 3 or 4 being Trp, Tyr or Phe;
    the amino acid in position 8 being Arg, Lys or His;
    the amino acid at positions 9, 10, 12 and 14, respectively, being any of the 20 naturally occurring amino acid residues; and
    b.
  • Trp Cys Cys Xaa Cys Tyr (SEQ ID NO. 81)
    1   2   3   4   5   6

    the amino acid at position 4 being any of the 20 naturally occurring amino acid residues; and
    c.
  • (SEQ ID NO: 82)
    Xaa Pro Gly Gly Gly Xaa Gly Xaa Phe
    1   2   3   4   5   6   7   8   9

    the amino acid at position 1 being Met or Ile;
    the amino acid at positions 6 and 8, respectively, is Leu, Ile or Val; and
    d.
  • (SEQ ID NO: 83)
    Gly Cys Xaa Xaa Arg Xaa Asp Trp Xaa
    1   2   3   4   5   6   7   8   9

    the amino acid at position 3 being any of the 20 naturally occurring amino acid residues;
    the amino acid at positions 4 and 6, respectively, being Trp, Tyr or Phe; and
    the amino acid at position 9 being Phe or Met;
  • The useful oligonucleotides also comprise nucleotide sequences complementary to the sequences mentioned.
  • In a preferred embodiment of the method of the invention, the oligonucleotide corresponds to a PCR primer selected from the PCR primers
  • sense:
    (SEQ ID NO: 84)
    5′-CCCCAAGCTTACIA/CGITAC/TTGGGAC/TTGC/TTGC/TAAA/G
    A/CC-3′
    antsense 1:
    (SEQ ID NO: 85)
    5′-CTAGTCTAGATAA/GCAIGCA/GCAA/GCACC-3′;
    antisense 2:
    (SEQ ID NO: 86)
    CTAGTCTAGAAAIAA/G/TICCIA/C/GICCICCIGG-3′;
    antisense 3:
    (SEQ ID NO: 87)
    5′-CTAGTCTAGAIAACCAA/GTCAA/G A/TAICG/TCC-3.
  • In a third aspect, the present invention provides an enzyme preparation which essentially consists of an enzyme having cellulytic activity and having the conserved regions found by the inventors, i.e., which comprises a peptide consisting of 7 amino acid residues having the following sequence (SEQ ID NOS: 105-107)
  • Xaa Thr Arg Xaa Phe Asp Xaa
    1   2   3   4   5   6   7;
    Xaa Thr Arg Xaa Tyr Asp Xaa
    1   2   3   4   5   6   7;
    and
    Xaa Thr Arg Xaa Trp Asp Xaa
    1   2   3   4   5   6   7

    wherein Xaa at position 4 is Trp, Tyr or Phe; and Xaa at positions 1 and 7 is any of the 20 naturally occurring amino acid residues.
  • This enzyme is obtainable from a strain belonging to Basidiomycotous Hymenomycetes (see FIG. 2), more preferably to the group consisting of the orders Agaricales, Auriculariales, and Aphyllophorales, even more preferably to the group consisting of the families Exidiaceae, Tricholomataceae, Coprinaceae, Schizophyllaceae, Bjerkanderaceae and Polyporaceae, especially to the group consisting of the genera Exidia, Crinipellis, Fomes, Panaeolus, Trametes, Schizophyllum, and Spongipellis.
  • Specific examples are endoglucanases obtainable from a strain belonging to the group consisting of the species Exidia glandulosa, Crinipellis scabella, Fomes fomentarius, and Spongipellis sp., more specific examples being Exidia glandulosa, CBS 277.96, Crinipellis scabella, CBS 280.96, Fomes fomentarius, CBS 276.96, and Spongipellis sp., CBS 283.96.
  • Exidia glandulosa was deposited at Centraalbureau voor Schimmelcultures, Oosterstraat 1, Postbus 273, NL-3740 AG Baarn, the Netherlands, on 12 Mar., 199, under the deposition number CBS 277.96; Crinipellis scabella was deposited at Centraalbureau voor Schimmelcultures on 12 Mar., 1996, under the deposition number CBS 280.96, Fomes fomentarius was deposited at Centraalbureau voor Schimmelcultures on 12 Mar., 1996, under the deposition number CBS 276.96, and Spongipellis sp. was deposited at Centraalbureau voor Schimmelcultures on 12 Mar., 1996, under the deposition number CBS 283.96, all deposited under the Budapest Treaty.
  • The enzyme preparation of the invention is also obtainable from a strain belonging to Chytridiomycota, preferably from a strain belonging to the class of Chytridiomycetes, more preferably belonging to the group consisting of the order Spizellomycetales, even more preferably to the family Spizellomycetaceae, especially belonging to the genus Rhizophlyctis. A specific example is a strain belonging to the species Rhizophlyctis rosea, more specifically to Rhizophlyctis rosea, CBS 282.96.
  • Rhizophlyctis rosea was deposited at Centraalbureau voor Schimmelcultures on 12 Mar. 1996, under the deposition number CBS 282.96, under the Budapest Treaty.
  • The enzyme preparation of the invention is also obtainable from a strain belonging to Zygomycota, preferably belonging to the class Zygomycetes, more preferably to the order Mucorales, even more preferably to the group of families consisting of Mucoraceae and Thamnidiaceae, especially belonging to the group consisting of the genera Rhizomucor, Phycomyces and Chaetostylum. Specific examples are strains belonging to the genera Rhizomucor pusillus, Phycomyces nitens, and Chaetostylum fresenii more specifically to Rhizomucor pusillus, IFO 4578, and Phycomyces nitens, IFO 4814 and Chaetostylum freseni, NRRL 2305.
  • Further, the enzyme preparation of the invention is also obtainable from a strain belonging to the group consisting of Archaeascomycetes, Discomycetes, Hemiascomycetes, Loculoascomycetes, and Plectomycetes, preferably belonging to the group consisting of the orders Pezizales, Rhytismatales, Dothideales, and Eurotiales. Especially, the enzyme is obtainable from a strain belonging the group consisting of the families Cucurbitariaceae, Ascobolaceae. Rhytismataceae, and Trichocomaceae, preferably belonging the group consisting of the genera Diplodia, Microsphaeropsis, Ulospora, Macrophomina, Ascobolus, Saccobolus, Penicillium, and Thermomyces. Specific examples are enzymes obtainable from a strain belonging the group consisting of the species Diplodia gossypina, Microsphaeropsis sp., Ulospora bilgramii, Aureobasidium sp. Macrophomina phaseolina, Ascobolus stictoides, Saccobolus diutellus, Peziza, Pencillium verruculosum, Penicillium chrysogenum, and Thermomyces verrucosus, more specifically Diplodia gossypina, CBS 274.96, Ulospora bilgramii NKBC 1444, Macrophomina phaseolina, CBS 281.96, Saccobolus dilutellus, CBS 275.96, Penicillium verruculosum, ATCC 62396, Penicillium chrysogenum, ATCC 9480, and Thermomyces verrucosus, CBS 285.96.
  • Diplodia gossypina was deposited at Centraalbureau voor Schimmelcultures on 12 Mar., 1996, under the deposition number CBS 274.96, Macrophomina phaseolina was deposited at Centraalbureau voor Schimmelcultures on 12 Mar., 1996, under the deposition number CBS 281.96, Saccobolus dilutellus was deposited at Centraalbureau voor Schimmelcultures on 12 Mar., 1996, under the deposition number CBS 275.96; Thermomyces verrucosus was deposited at Centraalbureau voor Schimmelcultures on 12 Mar., 1996, under the deposition number CBS 285.96; all under the Budapest Treaty.
  • Yet further, the enzyme is obtainable from a strain belonging to the group consisting of the orders Diaportales, Xylariales, Trichosphaeriales and Phyllachorales, preferably from a strain belonging to the group consisting of the families Xylariaceae, Vaisaceae, and Phyllachoraceae, more preferably belonging to the genera Diaporthe, Colletotrichum, Nigrospora, Xylaria, Nodulisporum and Poronia. Specific examples are the species Diaporthe syngenesia, Colletotrichum lagenarium, Xylaria hypoxylon, Nigrospora sp., Nodulisporum sp., and Poronia to punctata, more specifically Diaporthe syngenesia. CBS 278.96, Colletotrichum lagenarium, ATCC 52609, Nigrospora sp., CBS 272.96, Xylaria hypoxylon, CBS 284.96.
  • Diaporthe syngenesia was deposited at Centraalbureau voor Schimmelcultures on 12 Mar., 1996, under the deposition number CBS 278.96, Nigrospora sp. was deposited at Centraalbureau voor Schimmelcultures on 12 Mar., 1996, under the deposition number CBS 272.96, Xylaria hypoxylon was deposited at Centraalbureau voor Schimmelcultures on 12 Mar., 1996, under the deposition number CBS 284.96, all under the Budapest Treaty.
  • The enzyme is also obtainable from the unidentified fungal, mitosporic, coleomycetous deposited at Centraalbureau voor Schimmelcultures on 12 Mar. 1996, under the deposition numbers CBS 270.96, CBS 271.96 and CBS 273.96, respectively, under the Budapest Treaty.
  • The enzyme is also obtainable from a strain belonging to the group consisting of the genera Cylindrocarpon, Gliocladium, Nectria, Volutella, Sordaria, Scytalidium Thielavia, Syspastospora, Cladorrhinum, Chaetomium, Myceliphthora and Acremonium, especially from a strain belonging to the group consisting of the species Cylindrocarpon sp., Nectria pinea, Volutella colletotrichoides, Sordaria fimicola, Sordaria macrospora, Thielavia terrestris, Thielavia thermophila, Syspastospora boninensis, Cladorrhinum foecundissimum, Chaetomium murorum, Chaetomium virescens, Chaetomium brasiliensis, Chaetomium cunicolorum, Myceliophthora thermophila, Gliocladium catenulatum, Scytalidium thermophila, and Acremonium sp., more specifically from Nectria pinea, CBS 279.96. Volutella colletotrichoides, CBS 400.58, Sodaria fimicola, ATCC 52644, Sordaria macrospora, ATCC 60255, Thielavia terrestris, NRRL 8126, Thielavia thermophila, CCBS174.70, Chaetomium murorum, CBS 163.52, Chaetomium virescens, CBS 547.75, Chaetomium brasiliensis, CBS 122.65, Chaetomium cunicolorum, CBS 799.83, Syspastospora boninensis NKBC 1515, Cladorrhinum foecundissimum, ATCC 62373, Myceliophthora thermophila, CBS 117.65, Scytalidium thermophila, ATCC 28085, Gliocladium catenulatum, ATCC 10523, and Acremonium sp., CBS 478.94.
  • Nectria pinea was deposited at Centraalbureau voor Schimmelcultures on 12 Mar., 1996, under the deposition number CBS 279.96, and Acremonium Chaetomium sp. was deposited on 28 Sep. 1994 under the deposition number CBS 478.94, both according to the Budapest Treaty.
  • The enzyme is also obtainable from a strain belonging to the group consisting of the species Fusarium solani, Fusarium anguioides, Fusarium poae, Fusarium oxysporum sp. lycopersici, Fusarium oxysporum ssp. passiflora, Humicola nigrescens and Humicola grisea, especially Fusarium oxysporum ssp lycopersici CBS 645.78, Fusarium oxysporum ssp passiflora, CBS 744.79, Fusarium solani, IMI 107.511, Fusarium anguioides, IFO 4467, Fusarium poae, ATCC 60883, Humicola nigrescens, CBS 819.73 and Humicola grisea, ATCC 22726. It is to be noted that Humicola grisea is different from Humicola grisea var. thermoidea.
  • In a preferred embodiment, the enzyme preparation of the invention is derived from the disclosed classes, orders, families, genera and species and essentially consists of an enzyme comprising a first peptide consisting of 13 amino acid residues having the following sequence
  • (SEQ ID NO: 79)
    Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa
    1   2   3   4   5   6   7   8   9   10  11  12

    and a second peptide consisting of 5 amino acid residues having the following sequence
  • Trp Cys Cys Xaa Cys (SEQ ID NO: 80)
    1   2   3   4   5

    wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, and 12, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues.
  • Preferably, the amino acid residue at position 9 of the first sequence is selected from the group consisting of proline, threonine, valine, alanine, leucine, isoleucine, phenylalanine, glycine, cysteine, asparagine, glutamine, tyrosine, serine, methionine and tryptophan, more preferably from the group consisting of proline and threonine; the amino acid residue at position 10 of the first sequence which is selected from the group consisting of proline, threonine, valine, alanine, leucine, isoleucine, phenylalanine, glycine, cysteine, asparagine, glutamine, tyrosine, serine, methionine and tryptophan, preferably serine; the amino acid residue at position 12 of the first sequence is selected from the group consisting of proline, threonine, valine, alanine, leucine, isoleucine, phenylalanine, glycine, cysteine, asparagine, glutamine, tyrosine, serine, methionine and tryptophan, preferably from the group consisting of alanine and glycine; and the amino acid residue at position 4 of the second sequence is selected from the group consisting of proline, threonine, valine, alanine, leucine, isoleucine, phenylalanine, glycine, cysteine, asparagine, glutamine, tyrosine, serine, methionine, tryptophan, glutamic acid and aspartic acid, more preferably from the group consisting of alanine, glycine, and glutamine.
  • In further aspects, the present invention provides a DNA construct comprising a DNA sequence encoding an enzyme exhibiting endoglucanase activity, which DNA sequence comprises
  • a) the DNA sequence of SEQ ID NO: 1, 7, 9, 11, 13, 15, 21, or 25, respectively, or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae DSM 9770, DSM 10082, DSM 10080. DSM 10081, Escherichia coli, DSM 10512, DSM 10511, DSM 10571, DSM 10576, respectively; or
  • b) an analogue of the DNA sequence of SEQ ID NO: 1, 7, 9, 11, 13, 15, 21, or 25, respectively, or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae DSM 9770, DSM 10082, DSM 10080, DSM 10081, Escherichia coli, DSM 10512, DSM 10511, DSM 10571, DSM 10576, respectively, which
  • i) is homologous with the DNA sequence of SEQ ID NO: 1, 7, 9, 11, 13, 15, 21, or 25, respectively, or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae DSM 9770, DSM 10082, DSM 10080, DSM 10081 Escherichia coli DSM 10512, DSM 10511, DSM 10571, DSM 10576, respectively,
  • ii) hybridizes with the same oligonucleotide probe as the DNA sequence of SEQ ID NO: 1, 7, 9, 11, 13, 15, 21, or 25 respectively, or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae DSM 9770, DSM 10082, DSM 10080, DSM 10081, Escherichia coli, DSM 10512, DSM 10511, DSM 10571, DSM 10576, respectively,
  • iii) encodes a polypeptide which is homologous with the polypeptide encoded by a DNA sequence comprising the DNA sequence of SEQ ID NO: 1, 7, 9, 11, 13, 15, 21, or 25 respectively, or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae DSM 9770, DSM 10082, DSM 10080 DSM 10081, Escherichia coli, DSM 10512, DSM 10511, DSM 10571, DSM 10576, respectively,
  • iv) encodes a polypeptide which is immunologically reactive with an antibody raised against the purified endoglucanase encoded by the DNA sequence of SEQ ID NO: 1, 7, 9, 11, 13, 15, 21, or 25, respectively, or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae DSM 9770, DSM 10082, DSM 10080, DSM 10081, Escherichia coli, DSM 10512, DSM 10511, DSM 10571, DSM 10576, respectively.
  • Escherichia coli DSM 10512 was deposited under the Budapest Treaty on 2 Feb., 1996, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany).
  • Escherichia coli DSM 10511 was deposited under the Budapest Treaty on 2 Feb., 1996, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany).
  • Escherichia coli DSM 10571 was deposited under the Budapest Treaty on 6 Mar., 1996, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany).
  • Escherichia coli DSM 10576 was deposited under the Budapest Treaty on 12 Mar., 1996, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, to D-38124 Braunschweig, Germany).
  • Escherichia coli DSM 10583 was deposited under the Budapest Treaty on 13 Mar., 1996, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany).
  • Escherichia coli DSM 10584 was deposited under the Budapest Treaty on 13 Mar., 1996, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany).
  • Escherichia coli DSM 10585 was deposited under the Budapest Treaty on 13 Mar., 1996, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder leg 16, D-38124 Braunschweig, Germany).
  • Escherichia coli DSM 10586 was deposited under the Budapest Treaty on 13 Mar., 1996, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany).
  • Escherichia coli DSM 10587 was deposited under the Budapest Treaty on 13 Mar., 1996, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder leg 16, D-38124 Braunschweig, Germany).
  • Escherichia coli DSM 10588 was deposited under the Budapest Treaty on 13 Mar., 1996, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany).
  • Saccharomyces cerevisiae DSM 9770 was deposited under the Budapest Treaty on 24 Feb., 1995, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany).
  • Saccharomyces cerevisiae DSM 10082 was deposited under the Budapest Treaty on 30 Jun., 1995, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany).
  • Saccharomyces cerevisiae DSM 10080 was deposited under the Budapest Treaty on 30 Jun., 1995, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany).
  • Saccharomyces cerevisiae DSM 10081 was deposited under the Budapest Treaty on 30 Jun., 1995, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany).
  • The DNA construct of the invention relating to SEQ ID NO: 1 can be isolated from or produced on the basis of a DNA library of a strain of Myceliophthora, in particular a strain of M. thermophila, especially M. thermophila, CBS 117.65.
  • The DNA constructs of the invention relating to SEQ ID NOS: 7 and 9 can be isolated from or produced on the basis of a DNA library of a strain of Acrermonium, especially Acremonium sp., CBS 478.94.
  • The DNA construct of the invention relating to SEQ ID NO: 11 can be isolated from or produced on the basis of a DNA library of a strain of Thielavia in particular a strain of Thielavia terrestris, especially Thielavia terrestris, NRRL 8126.
  • The DNA construct of the invention relating to SEQ ID NO-13 can be isolated from or produced on the basis of a DNA library of a strain of Macrophomina, in particular a strain of M. phaseolina, especially M. phaseolina, CBS 281.96
  • The DNA construct of the invention relating to SEQ ID NO: 15 can be isolated from or produced on the basis of a DNA library of a strain of Crinipellis, in particular a strain of C. scabella, especially C. scabella, CBS 280.96.
  • The DNA construct of the invention relating to SEQ ID NO: 25 can be isolated from or produced on the basis of a DNA library of a strain of Sordaria, in particular a strain of Sordaria fimicola.
  • In the present context, the “analogue” of the DNA sequence of SEQ ID NO: 1, 7, 9, 11, 13, 15, 21, or 25, respectively, is intended to indicate any DNA sequence encoding an enzyme exhibiting endoglucanase activity, which has any or all of the properties i)-iv). The analogous DNA sequence
  • a) may be isolated from another or related (e.g., the same) organism producing the enzyme with endoglucanase activity on the basis of the DNA sequence of SEQ ID NO: 1, 7, 9, 11, 13, 15, 21, or 25, respectively, e.g., using the procedures described herein; the homologue may be an allelic variant of the DNA sequence comprising the DNA sequences shown herein, i.e., an alternative form of a gene that arises through mutation; mutations can be silent (no change in the encoded enzyme) or may encode enzymes having altered amino acid sequence; the homologue of the present DNA sequence may also be a genus or species homologue, i.e., encoding an enzyme with a similar activity derived from another species,
  • b) may be constructed on the basis of the DNA sequences of SEQ ID NO: 1, 7, 9, 11, 13, 15, 21, or 25, respectively, e.g., by introduction of nucleotide substitutions which do not give rise to another amino acid sequence of the endoglucanase encoded by the DNA sequence, but which correspond to the codon usage of the host organism intended for production of the enzyme, or by introduction of nucleotide substitutions which may give rise to a different amino acid sequence. However, in the tatter case amino acid changes are preferably of a minor nature, that is conservative amino acid substitutions that do not significantly affect the folding or activity of the protein, small deletions, typically of one to about 30 amino acid; small amino- or carboxyl-terminal extensions, such as an amino-terminal methionine residue, a small tinker peptide of up to about 20 residues, or a small extension that facilitates purification, such as a poly-histidine tract, an antigenic epitope or a binding domain. See in general Ford et al., Protein Expression and Purification 2: 95-107, 1991, Examples of conservative substitutions are within the group of basic amino acids (such as arginine, lysine, histidine), acidic amino acids (such as glutamic acid and aspartic acid), polar amino acids (such as glutamine and asparagine), hydrophobic amino acids (such as leucine, isoleucine, valine), aromatic amino acids (such as phenylalanine, tryptophan, tyrosine) and small amino acids (such as glycine, alanine, serine, threonine, methionine).
  • It will be apparent to persons skilled in the art that such substitutions can be made outside the regions critical to the function of the molecule and still result in an active polypeptide. Amino acids essential to the activity of the polypeptide encoded by the DNA construct of the invention, and therefore preferably not subject to substitution, may be identified according to procedures known in the art, such as site-directed mutagenesis or alanine-scanning mutagenesis (Cunningham and Wells, Science 244: 1081-1085 (1989)). In the latter technique mutations are introduced at every residue in the molecule, and the resultant mutant molecules are tested for biological (i.e., endoglucanase) activity to identify amino acid residues that are critical to the activity of the molecule. Sites of substrate-enzyme interaction can also be determined by analysis of crystal structure as determined by such techniques as nuclear magnetic resonance, crystallography or photoaffinity labeling, See, for example, de Vos et al., Science 255: 306-312 (1992); Smith et al. J. Mol. Biol. 224: 899-904 (1992); /Wlodaver et al., FEBS Lett. 309: 59-64 (1992).
  • The endoglucanase encoded by the DNA sequence of the DNA construct of the invention may comprise a cellulose binding domain (CBD) existing as an integral part of the encoded enzyme, or a CBD from another origin may be introduced into the endoglucanase enzyme thus creating an enzyme hybride. In this context, the term “cellulose-binding domain” is intended to be understood as defined by Peter Tomme et al. “Cellulose-Binding Domains, Classification and Properties” in “Enzymatic Degradation of Insoluble Carbohydrates”, John N. Saddler and Michael H. Penner (Eds.), ACS Symposium Series, No. 618, 1996. This definition classifies more than 120 cellulose-binding domains (CBDs) into 10 families (I-X), and it demonstrates that CBDs are found in various enzymes such as cellulases, xylanases, mannanases, arabinofuranosidases, acetyl esterases and chitinases. CBDs have also been found in algae, e.g., the red alga Porphyra purpurea as a non-hydrolytic polysaccharide-binding protein, for reference see Peter Tomme et al., supra. However, most of the CBDs are from cellulases and xylanases, CBDs are found at the N or C termini of proteins or are internal, Enzyme hybrids are known in the art, see, e.g., WO 90/00609 and WO 95/16782, and may be prepared by transforming into a host cell a DNA construct comprising at least a fragment of DNA encoding the cellulose-binding domain ligated, with or without a linker, to a DNA sequence encoding the enzyme of interest and growing the host cell to express the fused gene. Enzyme hybrids may be described by the following formula,

  • CBD-MR-X,
  • wherein CBD is the N-terminal or the C-terminal region of an amino acid sequence corresponding to at least the cellulose-binding domain; MR is the middle region (the linker), and may be a bond, or a short linking group preferably of from about 2 to about 100 carbon atoms, more preferably of from 2 to 40 carbon atoms; or is preferably from about 2 to about 100 amino acids, more preferably of from 2 to 40 amino acids; and X is an N-terminal or C-terminal region of a polypeptide encoded by the DNA sequence of the invention.
  • The homology referred to in i) above is determined as the degree of identity between the two sequences indicating a derivation of the first sequence from the second, The homology may suitably be determined by means of computer programs known in the art such as GAP provided in the GCG program package (Needleman, S. B. and Wunsch, C. D., Journal of Molecular Biology, 48: 443-453, 1970). Using GAP with the following settings for DNA sequence comparison: GAP creation penalty of 5.0 and GAP extension penalty of 0.3, the coding region of the DNA sequence exhibits a degree of identity preferably of at least 60%, more preferably at least 65%, more preferably at least 70%, even more preferably at least 80%, especially at least 90%, with the coding region of the DNA sequence of SEQ ID NO: 1, 7, 9, 11, 13, 15, or 21, respectively, or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae, DSM 9770, DSM 10082, DSM 10080, DSM 10081, Escherichia coli DSM 10512, DSM 10511, DSM 10571, or DSM 10576, respectively.
  • The hybridization referred to in ii) above is intended to indicate that the analogous DNA sequence hybridizes to the same probe as the DNA sequence encoding the endoglucanase enzyme under certain specified conditions which are described in detail in the Materials and Methods section hereinafter. The oligonucleotide probe to be used is the DNA sequence corresponding to the endoglucanase encoding part of the DNA sequence of SEQ ID NO: 1, 7, 9, 11, 13, 15 or 21, respectively, or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae, DSM 9770, DSM 10082, DSM 10080, DSM 10081, Escherichia coli, DSM 10512, DSM 10511, DSM 10571 or DSM 10576, respectively.
  • The homology referred to in iii) above is determined as the degree of identity between the two sequences indicating a derivation of the first sequence from the second. The homology may suitably be determined by means of computer programs known in the art such as GAP provided in the GCG program package (Needleman, S. B. and Wunsch, C. D., Journal of Molecular Biology, 48: 443-453, 1970). Using GAP with the following settings for polypeptide sequence comparison: GAP creation penalty of 3.0 and GAP extension penalty of 0.1 the polypeptide encoded by an analogous DNA sequence exhibits a degree of identity preferably of at least 55%, more preferably at least 80%, more preferably at least 858%, even more preferably at least 70%, more preferably at least 80%, especially at least 90%, with the enzyme encoded by a DNA construct comprising the DNA sequence of SEQ ID NO: 1, 7, 9, 11, 13, 15, 21, or 25, respectively, or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae, DSM 9770, DSM 10082, DSM 10080, DSM 10081, Escherichia coli, DSM 10512, DSM 10511, DSM 10571 or DSM 10576, respectively.
  • In connection with property iv) above it is intended to indicate an endoglucanase encoded by a DNA sequence isolated from strain Saccharomyces cerevisiae, DSM 9770. DSM 10082, DSM 10080: DSM 10081, Escherichia coli, DSM 10512, DSM 10511: DSM 10571 or DSM 105768 respectively, and produced in a host organism transformed with said DNA sequence or the corresponding endoglucanase naturally produced by Myceliophthora thermophila, Acremonium sp., Thielavia terrestis, Macrophomina phaseolina, Crinipellis scabella, Volutella colletotrichoides, or Sordaria fimicola, respectively. The immunological reactivity may be determined by the method described in the Materials and Methods section below.
  • In further aspects the invention relates to an expression vector harbouring a DNA construct of the invention, a cell comprising the DNA construct or expression vector and a method of producing an enzyme exhibiting endoglucanase activity which method comprises culturing said cell under conditions permitting the production of the enzyme, and recovering the enzyme from the culture.
  • In a still further aspect the invention relates to an enzyme exhibiting endoglucanase activity, which enzyme
  • a) is encoded by a DNA construct of the invention
  • b) produced by the method of the invention, and/or
  • c) is immunologically reactive with an antibody raised against a purified endoglucanase encoded by the DNA sequence of SEQ ID NO: 1, 7, 9, 11, 13, 15, or 21, respectively, or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae, DSM 9770, DSM 10082, DSM 10080, DSM 10081, Escherichia coli, DSM 10512, DSM 10511, DSM 10571 or DSM 10576, respectively.
  • The endoglucanase mentioned in c) above may be encoded by the DNA sequence isolated from the strain Saccharomyces cerevisiae, DSM 9770, DSM 10082, DSM 10080, DSM 10081, Escherichia coli, DSM 10512, DSM 10511, DSM 10571 or DSM 10576, respectively, and produced in a host organism transformed with said DNA sequence or the corresponding endoglucanase naturally produced by Myceliophthora thermophila, Acremonium sp., Thielavia terrestris, Macrophomina phaseolina, Crinipellis scabella, Volutella colletotrichoides or Sordaria fimicola, respectively.
  • Generally, in the present context the term “enzyme” is understood to include a mature protein or a precursor form thereof as well to a functional fragment thereof which essentially has the activity of the full-length enzyme. Furthermore, the term “enzyme” is intended to include homologues of said enzyme.
  • Homologues of the present enzyme may have one or more amino acid substitutions, deletions or additions. These changes are preferably of a minor nature, that is conservative amino acid substitutions that do not significantly affect the folding or activity of the protein, small deletions, typically of one to about 30 amino acids; small amino- or carboxyl-terminal extensions, such as an amino-terminal methionine residue, a small linker peptide of up to about 20-25 residues, or a small extension that facilitates purification, such as a poly-histidine tract, an antigenic epitope or a binding domain. See in general Ford et al., Protein Expression and Purification 2: 95-107 (1991). Examples of conservative substitutions are within the group of basic amino acids (such as arginine, lysine, histidine), acidic amino acids (such as glutamic acid and aspartic acid), polar amino acids (such as glutamine and asparagine), hydrophobic amino acids (such as leucine, isoleucine, valine), aromatic amino acids (such as phenylalanine, tryptophan, tyrosine) and small amino acids (such as glycine, alanine, serine, threonine, methionine).
  • It will be apparent to persons skilled in the art that such substitutions can be made outside the regions critical to the function of the molecule and still result in an active enzyme. Amino acids essential to the activity of the enzyme of the invention, and therefore preferably not subject to substitution, may be identified according to procedures known in the art, such as site-directed mutagenesis or alanine-scanning mutagenesis (Cunningham, 1989). In the latter technique mutations are introduced at every residue in the molecule, and the resultant mutant molecules are tested for cellulytic activity to identify amino acid residues that are critical to the activity of the molecule. Sites of ligand-receptor interaction can also be determined by analysis of crystal structure as determined by such techniques as nuclear magnetic resonance, crystallography or photoaffinity labelling. See, for example, de Vos et al., 1992; Smith et al., 1992, Wlodaver et al., 1992.
  • The homologue may be an allelic variant, i.e., an alternative form of a gene that arises through mutation, or an altered enzyme encoded by the mutated gene, but having substantially the same activity as the enzyme of the invention. Hence mutations can be silent (no change in the encoded enzyme) or may encode enzymes having altered amino acid sequence.
  • The homologue of the present enzyme may also be a genus or species homologue, i.e. an enzyme with a similar activity derived from another species.
  • A homologue of the enzyme may be isolated by using the procedures described herein.
  • Molecular Screening and, Cloning by Polymerase Chain Reaction (PCR)
  • Molecular screening for DNA sequences of the invention may be carried out by polymerase chain reaction (PC R) using genomic DNA or double-stranded cDNA isolated from a suitable source, such as any of the herein mentioned organisms, and synthetic oligonucleotide primers prepared on the basis of the DNA sequences or the amino acid sequences disclosed herein. For instance, it suitable oligonucleotide primers may be the primers described in the Materials and Methods section.
  • In accordance with well-known procedures, the PCR fragment generated in the molecular screening may be isolated and subcloned into a suitable vector. The PCR fragment may be used for screening DNA libraries by, e.g., colony or plaque hybridization.
    • Expression Cloning in Yeast
  • The DNA sequence of the invention encoding an enzyme exhibiting endoglucanase activity may be isolated by a general method involving
      • cloning, in suitable vectors, a DNA library from a suitable source, such as any of the herein mentioned organisms
      • transforming suitable yeast host cells with said vectors,
      • culturing the host cells under suitable conditions to express any enzyme of interest encoded by a clone in the DNA library,
      • screening for positive clones by determining any endoglucanase activity of the enzyme produced by such clones, and
      • isolating the enzyme encoding DNA from such clones.
  • The general method is further disclosed in WO 94/14953 the contents of which are hereby incorporated by reference. A more detailed description of the screening method is given in Example 1 below.
  • The DNA sequence coding for the enzyme may for instance be isolated by screening a cDNA library of Macrophomina phaseolina. Crinipellis scabella, Sordaria fimicola or Volutella colletotrichoides, and selecting for clones expressing the appropriate enzyme activity (i.e., endoglucanase activity) or from Escherichia coli DSM 10512 deposited under the Budapest Treaty on 2 Feb. 1996, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany), or from Escherichia coli DSM 10511 deposited under the Budapest Treaty on 2 Feb. 1996, at DSM, or from Escherichia coli DSM 10576, deposited under the Budapest Treaty on 12 Mar. 1996, at DSM; or from Escherichia coli DSM 10571 deposited under the Budapest Treaty on 6 Mar. 1996, at DSM; or by screening a cDNA library of Myceliphthora thermophila, CBS 117.65, Acremonium sp., CBS 478.94 or Thielavia terrestris, NRRL 8126, and selecting for clones expressing the appropriate enzyme activity (i.e., endoglucanase activity) or from Saccharomyces cerevisiae DSM 9770 deposited under the Budapest Treaty on 24 Feb. 1995, at DSM (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 16, D-38124 Braunschweig, Germany), or from Saccharomyces cerevisiae DSM 10082 deposited under the Budapest Treaty on 30 Jun., 1996, at DSM, from Saccharomyces cerevisiae DSM 10080 deposited under the Budapest Treaty on 30 Jun. 1995, or from Saccharomyces cerevisiae DSM 10081 deposited under the Budapest Treaty on 30 Jun. 1995, at DSM. The appropriate DNA sequence may then be isolated from the clone by standard procedures, e.g., as described in Example 1.
    • Nucleic Acid Construct
  • As used herein the term “nucleic acid construct” is intended to indicate any nucleic acid molecule of cDNA, genomic DNA, synthetic DNA or RNA origin. The term “construct” is intended to indicate a nucleic acid segment which may be single or double-stranded, and which may be based on a complete or partial naturally occurring nucleotide sequence encoding an enzyme of interest. The construct may optionally contain other nucleic acid segments.
  • The nucleic acid construct encoding the enzyme of the invention may suitably be of genomic or cDNA origin, for instance obtained by preparing a genomic or cDNA library and screening for DNA sequences coding for all or part of the enzyme by hybridization using synthetic oligonucleotide probes in accordance with standard techniques (cf. Sambrook et al., 1989).
  • The nucleic acid construct encoding the enzyme may also be prepared synthetically by established standard methods, e.g., the phosphoramidite method described by Beaucage and Caruthers (1981), or the method described by Matthes et al. (1984). According to the phosphoramidite method, oligonucleotides are synthesized, e.g., in an automatic DNA synthesizer, purified, annealed, ligated and cloned in suitable vectors.
  • Furthermore, the nucleic acid construct may be of mixed synthetic and genomic, mixed synthetic and cDNA or mixed genomic and cDNA origin prepared by ligating fragments of synthetic, genomic or cDNA origin (as appropriate), the fragments corresponding to various parts of the entire nucleic acid construct, in accordance with standard techniques.
  • The nucleic acid construct may also be prepared by polymerase chain reaction using specific primers, for instance as described in U.S. Pat. No. 4,683,202 or Saiki et al. (1988).
  • The nucleic acid construct is preferably a DNA construct which term will be used exclusively in this specification and claims,
    • Recombinant Vector
  • A recombinant vector comprising a DNA construct encoding the enzyme of the invention may be any vector which may conveniently be subjected to recombinant DNA procedures, and the choice of vector will often depend on the host cell into which it is to be introduced. Thus, the vector may be an autonomously replicating vector, i.e., a vector which exists as an extrachromosomal entity, the replication of which is independent of chromosomal replication, e.g., a plasmid. Alternatively, the vector may be one which, when introduced into a host cell, is integrated into the host cell genome and replicated together with the chromosome(s) into which it has been integrated.
  • The vector is preferably an expression vector in which the DNA sequence encoding the enzyme of the invention is operably linked to additional segments required for transcription of the DNA In general, the expression vector is derived from plasmid or viral DNA, or may contain elements of both. The term, “operably linked” indicates that the segments are arranged so that they function in concert for their intended purposes, e.g., transcription initiates in a promoter and proceeds through the DNA sequence coding for the enzyme.
  • The promoter may be any DNA sequence which shows transcriptional activity in the host cell of choice and may be derived from genes encoding proteins either homologous or heterologous to the host cell.
  • Examples of suitable promoters for use in yeast host cells include promoters from yeast glycolytic genes (Hitzeman et al., J. Biol. Chem. 255: 12073-12080 (1980); Alber and Kawasaki, J. Mol. Appl. Gen. 1: 419-434 (1982)) or alcohol dehydrogenase genes (Young et al., in Genetic Engineering of Microorganisms for Chemicals (Hollaender et at, eds.), Plenum Press, New York, 1982), or the TPI1 (U.S. Pat. No. 4,599,311) or ADH2-4c (Russell et al., Nature 304: 652-654 (1983)) promoters.
  • Examples of suitable promoters for use in filamentous fungus host cells are, for instance, to the ADH3 promoter (McKnight et a<. The EMBO J. 4: 2093-2099 (1985)) or the tpiA promoter. Examples of other useful promoters are those derived from the gene encoding A. oryzae TAKA amylase, Rhizomucor miehei aspartic proteinase, A. niger neutral alpha-amylase, A. niger acid stable alpha-amylase, A. niger or A. awamori glucoamylase (gluA), Rhizomucor miehei lipase, A. oryzae alkaline protease, A. oryzae triose phosphate isomerase or A. nidulans acetamidase. Preferred are the TAKA-amylase and gluA promoters.
  • Examples of suitable promoters for use in bacterial host cells include the promoter of the Bacillus stearothermophilus maltogenic amylase gene, the Bacillus licheniformis alpha-amylase gene, the Bacillus amyloliquefaciens BAN amylase gene, the Bacillus subtilis alkaline protease gen, or the Bacillus pumilus xylosidase gene, or by the phage Lambda PR or PL promoters or the E. coli lac, trp, or tac promoters.
  • The DNA sequence encoding the enzyme of the invention may also, if necessary, be operably connected to a suitable terminator.
  • The recombinant vector of the invention may further comprise a DNA sequence enabling the vector to replicate in the host cell in question.
  • The vector may also comprise a selectable marker, e.g., a gene the product of which complements a defect in the host cell, such as the gene coding for dihydrofolate reductase (DHFR) or the Schizosaccharomyces pombe TPI gene (described by P. R. Russell, Gene 40, 1985, pp. 125-130). For filamentous fungi, selectable markers include amdS pyrG, argB, niaD, sC.
  • To direct an enzyme of the present invention into the secretory pathway of the host cells, a secretory signal sequence (also known as a leader sequence, prepro sequence or pre sequence) may be provided in the recombinant vector. The secretory signal sequence is joined to the DNA sequence encoding the enzyme in the correct reading frame. Secretory signal sequences are commonly positioned 5′ to the DNA sequence encoding the enzyme, The secretory signal sequence may be that normally associated with the enzyme or may be from a gene encoding another secreted protein.
  • For secretion from yeast cells, the secretory signal sequence may encode any signal peptide which ensures efficient direction of the expressed enzyme into the secretory pathway of the cell. The signal peptide may be a naturally occurring signal peptide, or a functional part thereof, or it may be a synthetic peptide. Suitable signal peptides have been found to be the alpha-factor signal peptide (cf. U.S. Pat. No. 4,870,008), the signal peptide of mouse salivary amylase (cf. O. Hagenbuchle et al., Nature 289, 1981, pp. 643-646), a modified carboxypeptidase signal peptide (cf. L. A. Valls et al., Cell 48, 1987, pp. 887-897), the yeast BAR1 signal peptide (cf. WO 87/02670), or the yeast aspartic protease 3 (YAP3) signal peptide (cf. M. Egel-Mitani et al., Yeast 6, 1990, pp. 127-137).
  • For efficient secretion in yeast, a sequence encoding a leader peptide may also be inserted downstream of the signal sequence and upstream of the DNA sequence encoding the enzyme, The function of the leader peptide is to allow the expressed enzyme to be directed from the endoplasmic reticulum to the Golgi apparatus and further to a secretory vesicle for secretion into the culture medium (i.e., exportation of the enzyme across the cell wall or at least through the cellular membrane into the periplasmic space of the yeast cell). The leader peptide may be the yeast alpha-factor leader (the use of which is described in, e.g., U.S. Pat. No. 4,546,082, EP 16 201, EP 123 294, EP 123 544 and EP 163 529). Alternatively, the leader peptide may be a synthetic leader peptide, which is to say a leader peptide not found in nature. Synthetic leader peptides may, for instance, be constructed as described in WO 89102463 or WO 92/11378.
  • For use in filamentous fungi, the signal peptide may conveniently be derived from a gene encoding an Aspergillus sp. amylase or glucoamylase, a gene encoding a Rhizomucor miehei lipase or protease, a Humicola lanuginosa lipase. The signal peptide is preferably derived from a gene encoding A. oryzae TAKA amylase, A. niger neutral alpha-amylase, A. niger acid-stable amylase, or A. niger glucoamylase.
  • The procedures used to ligate the DNA sequences coding for the present enzyme, the promoter and optionally the terminator and/or secretory signal sequence, respectively, and to insert them into suitable vectors containing the information necessary for replication, are well known to persons skilled in the art (cf., for instance, Sambrook et al., cit.).
    • Host Cells
  • The DNA sequence encoding the present enzyme introduced into the host cell may be either homologous or heterologous to the host in question. If homologous to the host cell, i.e., produced by the host cell in nature, it will typically be operably connected to another promoter sequence or, if applicable, another secretory signal sequence and/or terminator sequence than in its natural environment. The term “homologous” is intended to include a cDNA sequence encoding an enzyme native to the host organism in question. The term “heterologous” is intended to include a DNA sequence not expressed by the host cell in nature. Thus, the DNA sequence may be from another organism, or it may be a synthetic sequence.
  • The host cell into which the DNA construct or the recombinant vector of the invention is introduced may be any cell which is capable of producing the present enzyme and includes bacteria, yeast, fungi and higher eukaryotic cells.
  • Examples of bacterial host cells which, on cultivation, are capable of producing the enzyme of the invention are gram-positive bacteria such as strains of Bacillus, such as strains of B. subtilis, B. licheniformis, B. lentus, B. brevis, B. stearothermophilus, B. alkalophilus, B. amyloliquefaciens. B. coagulans, B. circulans, B. lautus, B. megatherium or B. thuringiensis, or strains of Streptomyces, such as S. lividans or S. murinus, or gram-negative bacteria such as Echerichia coli. The transformation of the bacteria may be effected by protoplast transformation or by using competent cells in a manner known per se (cf. Sambrook et al., supra).
  • When expressing the enzyme in bacteria such as E. coli, the enzyme may be retained in the cytoplasm, typically as insoluble granules (known as inclusion bodies), or may be directed to the periplasmic space by a bacterial secretion sequence. In the former case, the cells are lysed and the granules are recovered and denatured after which the enzyme is refolded by diluting the denaturing agent. In the latter case, the enzyme may be recovered from the periplasmic space by disrupting the cells, e.g., by sonication or osmotic shock, to release the contents of the periplasmic space and recovering the enzyme.
  • Examples of suitable yeasts cells include cells of Saccharomyces spp. or Schizosacharomyces spp., in particular strains of Saccharomyces cerevisiae or Saccharomyces kluyveri. Methods for transforming yeast cells with heterologous DNA and producing heterologous enzymes therefrom are described, e.g., in U.S. Pat. No. 4,599,311, U.S. Pat. No. 4,931,373, U.S. Pat. Nos. 4,870,008, 5,037,743, and U.S. Pat. No. 4,845,075, all of which are hereby incorporated by reference. Transformed cells are selected by a phenotype determined by a selectable marker, commonly drug resistance or the ability to grow in the absence of a particular nutrient, e.g., leucine. A preferred vector for use in yeast is the POT1 vector disclosed in U.S. Pat. No. 4,931,373. The DNA sequence encoding the enzyme of the invention may be preceded by a signal sequence and optionally a leader sequence, e.g., as described above. Further examples of suitable yeast cells are strains of Kluyveromyces such as K. lactis, Hansenula, e.g., H. polymorpha, or Pichia, e.g., P. pastoris (cf. Gleeson et al., S. Gen. Microbiol. 132, 1986, pp. 3459-3465; U.S. Pat. No. 4,882,279).
  • Examples of other fungal cells are cells of filamentous fungi, e.g., Aspergillus spp., Neurospora spp., Fusarium spp. or Trichoderma spp., in particular strains of A. oryzae, A. nidulans, A. niger, or Fusarium graminearum. The use of Aspergillus spp. for the expression of proteins is described in, e.g., EP 272 277, ER 230 023. The transformation of F. oxysporum may, for instance, be carried out as described by Malardier et al., 1989, Gene 78; 147-156.
  • When a filamentous fungus is used as the host cell, it may be transformed with the DNA construct of the invention, conveniently by integrating the DNA construct in the host chromosome to obtain a recombinant host cell. This integration is generally considered to be an advantage as the to DNA sequence is more likely to be stably maintained in the cell. Integration of the DNA constructs into the host chromosome may be performed according to conventional methods, e.g., by homologous or heterologous recombination.
  • The transformed or transfected host cell described above is then cultured in a suitable nutrient medium under conditions permitting the expression of the present enzyme, after which the resulting enzyme is recovered from the culture.
  • The medium used to culture the cells may be any conventional medium suitable for growing the host cells, such as minimal or complex media containing appropriate supplements. Suitable media are available from commercial suppliers or may be prepared according to published recipes (e.g., in catalogues of the American Type Culture Collection). The enzyme produced by the cells may then be recovered from the culture medium by conventional procedures including separating the host cells from the medium by centrifugation or filtration, precipitating the proteinaceous components of the supernatant or filtrate by means of a salt, e.g., ammonium sulphate, purification by a variety of chromatographic procedures, e.g., ion exchange chromatography, ge filtration chromatography, affinity chromatography, or the like, dependent on the type of enzyme in question.
  • In a still further aspect, the present invention relates to a method of producing an enzyme according to the invention, wherein a suitable host cell transformed with a DNA sequence encoding the enzyme is cultured under conditions permitting the production of the enzyme, and the resulting enzyme is recovered from the culture.
    • Enzyme Screening Driven by Taxonomy as Well as Ecology,
  • A powerful tool like the molecular screening disclosed herein, designed to detect and select said type of interesting enzymes, can still not stand on its own. In order to maximize the chances of making interesting discoveries the molecular screening approach was in the present investigation combined with careful selection of which fungi to screen. The selection was done through a thorough insight in the identification of fungi, in taxonomical classification and in phylogenetic relationships.
  • A taxonomic hot spot for production of cellulytic enzymes can further only be fully explored if also the ecological approach is included. Thorough knowledge about the adaptation to various substrates (especially saprotrophic, necrotrophic or biotrophic degradation of plant materials) are prerequisites for designing an intelligent screening and for managing a successful selection of strains and ecological niches to be searched.
  • Both the taxonomy and the ecological approach disclosed herein aim at maximizing discovery of said enzymes in the molecular screening program. However, still several hundreds (or if all preliminary work is included) several thousand fungi have been brought in culture in order to detect the 53 hits of said type of cellulytic enzyme here reported.
  • The screening and cloning may be carried out using the following:
    • Materials and Methods List of Organisms:
  • Saccharomyces cerevisiae, DSM 9770, DSM 10082, DSM 10080, DSM 10081, or Escherichia coli, DSM 10512, DSM 10511, DSM 10571, DSM 10576, respectively, containing the plasmid comprising the full length DNA sequence, coding for the endoglucanase of the invention, in the shuttle vector pYES 2.0.
  • Escherichia coli DSM 10583, 10584, 10585, 10586, 10587, and 10588.
  • Diplcodia gossypina Cooke
    • Deposit of Strain, Acc No: CBS 274.96 Classification: Ascomycota, Loculoascomycetes, Dothideales, Cucurbitariaceae
  • Ulospora bilgramii (Hawksw. et at) Hawksw. et al.,
  • Acc No of strain: NKBC 1444, Nippon University, (Prof. Tubaki collection)
    Classification: Ascomycota, Loculoascomycetes, Dothideales, (family unclassified)
  • Microsphaeropsis sp.
  • Isolated from: Leaf of Camellia japonica (Theaceae, Guttiferales), grown in Kunming Botanical garden, Yunnan Province, China
    Classification: Ascomycota, Loculoascomycetes, Dothideales, (family unclassified)
  • Macrophomina phaseolina (Tassi) Goidannich
  • Syn: Rhizoctonia bataticola
    • Deposit of Strain, Acc No.: CBS 281.96
  • Isolated from seed of Glycine max (Leguminosa), cv CMM 60, grown in Thailand, 1990
    • Classification: Ascomycota, Discomycetes, Rhytismatales, Rhytismataceae
  • Ascobolus stictoideus Speg.
  • Isolated from goose dung, Svalbard, Norway
    • Classification: Ascomycota, Discomycetes, Pezizales, Ascobolaceae
  • Saccobolus dilutellus (Fuck.) Sacc.
  • Deposit of strain: Acc No CBS 275.96
    • Classification: Ascomycota, Discomycetes, Pezizales, Ascobolaceae
  • Penicillium verruculosum Peyronel
  • Ex on Acc No of species. ATCC 62396
    • Classification, Ascomycota, Plectomycetes, Eurotiales, Trichocomaceae
  • Penicillium chrysogenum Thom
    • Acc No of Strain: ATCC 9480 Classification: Ascomycota, Plectomycetes, Eurotiales, Trichocomaceae
  • Thermomyces verrucosus Pugh et al
    • Deposit of Strain, Acc No.: CBS 285.96
  • Classification: Ascomycota, Plectomycetes, Eurotiales, (family unclassified affiliation based on 18S RNA, sequencing and homologies)
  • Xylaria hypoxylon L. ex Greville
    • Deposit of Strain, Acc No: CBS 284.96 Classification Ascomycota, Pyrenomycetes, Xylariales, Xylariaceae
  • Poronia punctata (Fr. ex L.) Fr,
    • Classification: Ascomycota, Pyrenomycetes, Xylariales, Xylariaceae
  • Nodulisporum sp
  • Isolated from leaf of Camellia reticulatá (Theaceae, Guttiferales), grown in Kunming Botanical Garden, Yunnan Province, China
    • Classification, Ascomycota, Pyrenomycetes, Xylariales, Xylariaceae
  • Cylindrocarpon sp
  • Isolated from marine sample, the Bahamas
    Classification, Ascomycota, Pyrenomycetes., Hypocreales (unclassified)
  • Acremonium sp
  • Deposit of Strain, Acc. No.: CBS 478.94
    • Classification: Ascomycota, Pyrenomycetes, Hypocreales, Hypocreaceae
  • Fusarium anguioides Sherbakoff
  • Acc No of strain: IFO 4467
    • Classification Ascomycota, Pyrenomycetes, Hypocreales, Hypocreaceae
  • Fusarium poae (Peck) Wr.
  • Ex on Acc No of species: ATCC 60883
    • Classification: Ascomycota, Pyrenomycetes, Hypocreales, Hypocreaceae
  • Fusarium solani (Mart.)Sacc.emnd.Snyd & Hans.
  • Acc No of strain: IMI 107.511
    • Classification, Ascomycota, Pyrenomycetes, Hypocreales, Hypocreaceae
  • Fusarium oxysporum ssp lycopersici (Sacc.)Snyd. & Hans.
  • Acc No of strain: CBS 645.78
    • Classification: Ascomycota, Pyrenomycetes, Hypocreales, Hypocreaceae
  • Fusarium oxysporum ssp passiflora
  • Acc No of strain: CBS 744.79
    • Classification: Ascomycota, Pyrenomycetes, Hypocreales, Hypocreaceae
  • Gliocladium catenulatum Gilman & Abbott
  • Acc. No. of strain: CBS 227.48
    • Classification: Ascomycota, Pyrenomycetes, Hypocreales, Hypocreaceae
  • Nectria pinea Dingley
  • Deposit of Strain. Acc. No. CBS 279.96
    • Classification: Ascomycota, Pyrenomycetes, Hypocreales, Nectriaceae
  • Volutella colletotrichoides
    • Acc No of Strain: CBS 400.58
  • Classification: Ascomycota, Pyrenomycetes, Hypocreales (unclassified)
  • Sordaria macrospora Auerswald
  • Ex on Acc No of species: ATCC 60255
    • Classification: Ascomycota, Pyrenomycetes, Sordariales, Sordariaceae
  • Sordaria fimicola (Roberge) Cesati et De Notaris
  • Ex on Acc. No. for the species: ATCC 52644
    Isolated from dung by H. Dissing, ISP, KU, Denmark
    • Classification: Ascomycota, Pyrenomycetes, Sordariales, Sordariaceae
  • Humicola grisea Traeen
  • ex on Acc No for the species: ATCC 22726
    • Source: Hatfield Polytechnic
  • Classification: Ascomycota, Pyrenomycetes, Sordariales, (fam. unclassified)
  • Humicola nigrescens Omvik
  • Acc No of strain: CBS 819.73
    Classification: Ascomycota, Pyrenomycetes, Sordariales, (fam. unclassified)
  • Scytalidium thermophilum (Cooney et Emerson) Aushtick
  • Acc No of strain: ATCC 28085
    Classification: Ascomycota, Pyrenomycetes, Sordariales, (fam. unclassified)
  • Thielavia thermophila Fergus et Sinden
  • (syn Corynascus thermophilus)
    Acc No of strain: CBS 174.70, IMI 145.136
    • Classification: Ascomycota, Pyrenomycetes, Sordariales, Chaetomiaceae
  • Isolated from Mushroom compost
  • Thielavia terrestris (Appinis) Malloch et Cain
  • Acc No of strain: NRRL8126
    • Classification: Ascomycota, Pyrenomycetes, Sordariales, Chaetomiaceae
  • Cladorrhinum foecundissimum Saccardo et Marchal
  • Ex on Acc No of species, ATCC 62373
    • Classification: Ascomycota, Pyrenomycetes, Sordariales, Lasiosphaeriaceae
  • Isolated from leaf of Selandin sp, (Compositaceae, Asterales), Dallas Mountain, Jamaica
  • Syspastospora boninensis
  • Acc No of strain: NKBC 1515 (Nippon University, profe Tubaki Collection)
    • Classification, Ascomycota, Pyrenomycetes, Sordariales, Cerastomataceae
  • Chaetomium cuniculorum Fuckel
  • Acc. No. of strain: CBS 799.83
    • Classification: Ascomycota, Pyrenomycetes, Sordariales, Chaetomiaceae
  • Chaetomium brasiliense Batista et Potual
  • Acc No of strain: CBS 122.65
    • Classification: Ascomycota, Pyrenomycetes, Sordariales, Chaetomiaceae
  • Chaetomium murorum Corda
  • Acc No of strain: CBS 163.52
    • Classification: Ascomycota, Pyrenomycetes, Sordariales, Chaetomiaceae
  • Chaetomium virescens (von Arx) Udagawa
  • Acc. No. of strain: CBS 547.75
    • Classification: Ascomycota, Pyrenomycetes, Sordariales, Chaetomiaceae
  • Myceliophthora thermophila (Apinis) Oorschot
    • Deposit of Strain, Acc No: CBS 117.65 Classification: Ascomycota, Pyrenomycetes, Sordariales, Chaetomiaceae
  • Nigrospora sp
  • Deposit of strain, Acc No: CBS 272.96
    Isolated from leaf of Artocarpus altilis Moraceae, Urticales grown in Christiana, Jamaica
    Classification: Ascomycota, Pyrenomycetes, Trichosphaeriales, (family unclassified)
  • Nigrospora sp
  • Isolated from leaf of Pinus yuannanensis, Botanical Garden, Kuning, Yunnan,
    • Classification: Ascomycota, Pyrenomycetes, Trichosphaeriales, Abietaceae, Pinales.
  • Diaporthe syngenesia
  • Deposit of strain, Acc No: CBS 278.96
    • Classification: Ascomycota, Pyrenomycetes, Diaporthales, Valsaceae
  • Colletotrichum lagenarium (Passerini) Ellis et Halsted
  • syn Glomerella cingulata var orbiculare Jenkins et Winstead
    Ex on acc No of species: ATCC 52609
    • Classification, Ascomycota, Pyrenomycetes, Phyllachorales
  • Exidia glandulosa Fr.
  • Deposit of Strain. Acc No: CBS 27796
    • Classification: Basidiomycota, Hymenormycetes, Auriculariales, Exidiaceae
  • Crinipellis scabella (Alb. & Schw.; Fr)Murr
  • Deposit of strain: Acc No CBS 280.96
    • Classification: Basidiomycota, Hymenomycetes, Agaricales,
  • Panaeolus retirgis (Fr.) Gill.
  • Acc. No. of strain: CBS 275.47
    • Classification: Basidiomycota, Hymenomycetes, Agaricales, Coprinaesae
  • Fomes fomentarius (L.) Fr,
  • Deposit of strain: Acc No. CBS 276.96
    • Classification: Basidiomycota, Hymenomycetes, Aphyllophorales, Fomitaceae
  • Spongipellis Sp.
    • Deposit of Strain: Acc No CBS 283.96 Classification: Basidiomycota, Hymenomycetes, Aphyllophorales,
  • Bjerkanderaceae (identified and affiliated taxonomically by 18S sequence and homology)
  • Trametes sanguinea (Fr.) Lloyd
  • syn: Polyporus sanguineus; Pycnoporus sanguineus (L.:Fr.) Murril
    Acc No of strain: AKU 5062 (Kyoto University Culture Collection)
    • Classification, Basidiomycota, Aphyllophorales, Polyporaceae
  • Schizophyllum commune Fr
  • Acc. No. of species: ATCC 38548
    • Classification: Basidiomycota, Aphyllophorales, Schizophyllaceae
  • Rhizophlyctis rosea (de Bary & Wor) Fischer
    • Deposit of Strain: Acc No.: CBS 282.96 Classification: Chytridiomycota, Chytridiomycetes, Spizellomycetales, Spizellomycetaceae
  • Rhizomucor pusillus (Lindt) Schipper
  • syn: Mucor pusillus
    Acc No of strain: IFO 4578
    Ex on Acc No of species: ATCC 46883
    • Classification Zygomycota, Zygomycetes, Mucorales, Mucoraceae
  • Phycomyces nitens (Kunze) van Tieghem & Le Monnier
  • Acc No of strain: IFO 4814
    Ex on Aco No of species: ATCC 16327
    • Classification; Zygomycota, Zygomycetes, Mucorales, Mucoraceae
  • Chaetostylum fresenii van Tieghem & Le Monnier
  • syn. Helicostylum fresenii
    Acc No of strain NRRL 2305
    • Classification: Zygomycota, Zygomycetes, Mucorales, Thamnidiaceae Unclassified:
  • Trichothecium roseum
  • Acc No of strain: IFO 5372
  • Coniothecium sp
  • Endophyte, isolated from leaf of unidentifed higher plant, growing in Kunming, Yunnan, China
    • Unclassified and Un-identified:
  • Deposit of strain, Acc No.: CBS 271.96
  • Isolated from leaf of Artocarpus altillis (Moraceae, Urticales), grown in Christiana, Jamaica
  • Deposit of strain, Acc No.; CBS 273.96
  • Isolated from leaf of Pimenta dioica (Myrtaceae, Myrtales) grown in Dallas Mountain, Jamaica
  • Deposit of strain: CBS 270.96
  • Isolated from leaf of Pseudocalymma alliaceum (Bignoniaceae, Solanales) growing in Dallas Mountain, Jamaica
    Other strains:
  • Escherichia coli MC1061 and DH10B.
  • Yeast strain: The Saccharomyces cerevisiae strain used was W3124 (MATα; ura 3-52, leu 2-3, 112, h is 3-D200; pep 4-1137; prc1; HIS3; prb2: LEU2; cir+).
    • Plasmids:
  • The Aspergillus expression vector pH D414 is a derivative of the plasmid p775 (described in EP 238 023). The construction of pHD414 is further described in WO 93/11249.
  • pYES 2.0 (Invitrogen)
    pA2C477, pA2C193, pA2C357, pA2C371, pA2C385, pA2C475, pA2C488, pA2C502 (See example 1, 2, 3 and 4),
    • Isolation of the DNA Sequence of SEQ ID NO: 1, 7, 9, 1113, 15, 21, or 25, Respectively:
  • The full length DNA sequence, comprising the cDNA sequence of SEC ID NO: 1, 7, 9, 11, 13, 15, 21, or 25, respectively, coding for the endoglucanase of the invention, can be obtained from the deposited organism S. cerevisiae, DSM 9770, DSM 10082, DSM 10080, DSM 10081, E. coli, DSM 10512, DSM 10511, DSM 10571 or DSM 10576, respectively, by extraction of plasmid DNA by methods known in the art (Sambrook et al. (1989) Molecular cloning: A laboratory manual, Cold Spring Harbor lab., Cold Spring Harbor, N.Y.).
  • PCR primers for molecular screening of cellulases of the present invention:
  • The four degenerate, deoxyinosine-containing oligonucleotide primers (sense; s and antisense; as1, as2 and as3) corresponding to four highly conserved amino acid regions found in the deduced amino acid sequences of Thielavia terrestris cellulase, Myceliophthora thermophilum cellulase, and two cellulases from Acremonium sp. The residues are numbered according to the Myceliophthora thermophilum sequence. The deoxyinosines are depicted by an I in the primer sequences, and the restriction sites are underlined.
  • 27                               35
    NH2- Thr Arg Tyr Trp Asp Cys Cys Lys Pro/Thr -COOH (SEQ ID NO: 79)
    S 5′- CCCCAAGCCTT ACI AGI TAC TGG GAC TGC TGC AAA AC -3′ (SEQ ID NO: 84)
    HindIII     C     T       T   T   T   G C
    106                 111
    NH2- Trp Cys Cys Ala Cys Tyr -COOH (SEQ ID NO: 81)
    as1 3′-  CC ACA ACA CGI ACA AT    AGATCTGATC -5′ (SEQ ID NO: 85)
          G   G       G         XbaI
    145                                   152
    NH2- Pro Gly Gly Gly Leu/Val Gly Ile/Leu Phe -COOH (SEQ ID NO: 82)
    as2 3′- GGI CCI CCI CCI AAI     CCI AAI     AA    AGATCTGATC -5′ (SEQ ID NO: 86)
                 C               G
                 G                T
    193                             198
    NH2- Trp Arg Phe/Tyr Asp Trp Phe -COOH (SEQ ID NO: 83)
    as3 3′-  CC CGI AAA     CTA ACC AAA  AGATCTGATC -5′ (SEQ ID NO: 87)
        T    TG       G       G   XbaI
    • Molecular Screening by Polymerase Chain Reaction (PCR):
  • In Vitro Amplification of Genomic DNA and Double-Stranded cDNA.
  • Directional, double-stranded cDNA was synthesized from 5 micrograms of poly(A)+ RNA as described below. Genomic DNA was isolated according to Yelton et al.
  • Approximately 10 to 20 ng of double-stranded, cellulase-induced cDNA or 100 to 200 ng of genomic DNA from a selection of fungal strains was PCR amplified in PCR buffer (10 mM Tris-HCl, pH 8.3, 50 mM KCl, 1.5 mM MgCl2, 0.01% (w/v) gelatin) containing 200 micro-M of each dNTP and 100 pmol of each degenerate primer in three combinations,
  • 1) sense,
    (SEQ ID NO: 84)
    5′-CCCCAAGCTTACIA/CGITAC/TTGGGAC/TTGC/TTGC/TAAA/G
    A/CC-3′
    antisense
    1,
    (SEQ ID NO: 85)
    5′-CTAGTCTAGATAA/GCAIGCA/GCAA/GCACC-3′;
    or
    2) sense,
    (SEQ ID NO: 84)
    5′-CCCCAAGCTTACIA/CGITAC/TTGGGAC/TTGC/TTGC/TAAA/G
    A/CC-3′
    antisense
    2,
    (SEQ ID NO: 86)
    CTAGTCTAGAAAIAA/G/TICCIAA/C/GICCICCICCIGG-3′;
    or
    3) sense,
    (SEQ ID NO: 84)
    5′-CCCCAAGCTTACIA/CGITAC/TTGGGAC/TTGC/TTGC/TAAA/G
    A/CC-3′
    antisense
    3,
    (SEQ ID NO: 87)
    5′-CTAGTCTAGAIAACCAA/GTCAA/G A/TAICG/TCC-3;

    a DNA thermal cycler (Landgraf, Germany) and 2.5 units of Taq polymerase (Perkin-Elmer, Cetus, USA). Thirty cycles of PCR were performed using a cycle profile of denaturation at 94° C. for 1 min, annealing at 64° C. for 2 min, and extension at 72° C. for 3 min. Ten microliter aliquots of the amplification products were analyzed by electrophoresis in 3% agarose gels (NuSieve, FMC) with HaeIII-digested φ174 RF DNA as a size marker.
    • Direct Sequencing of the PCR Products.
  • Eighty microliter aliquots of the PCR products were purified using the QIAquick PCR purification kit (Qiagen, USA) according to the manufacturers instructions. The nucleotide sequences of the amplified PCR fragments were determined directly on the purified PCR products by the dideoxy chain-termination method, using 50-150 ng template, the Taq deoxy-terminal cycle sequencing kit (Perkin-Elmer, USA), fluorescent labeled terminators and 5 pmol of the sense primer: 5′-CCCCAAGCTTAClA/CGITAC/TTGGGAC/TT-GC/TTGC/TAAA/G A/CC-3′ (SEQ ID NO: 84). Analysis of the sequence data was performed according to Devereux at alt
    • Cloning by Polymerase Chain Reaction (PCR): Subcloning of PCR Fragments.
  • Twenty five microliter aliquots of the PCR products generated as described above were electrophoresed in 0.8% low gelling temperature agarose (SeaPlaque GTG, FMC) gels, the relevant fragments were excised from the gels, and recovered by agarase treatment by adding 0.1 vol of 10× agarase buffer (New England Biolabs) and 2 units per 100 microliters molten agarose to the sample, followed by incubation at 45° C. for 1.5 h. The sample was phenol and chloroform extracted, and precipitated by addition of 2 vols of 96% EtOH and 0.1 of 3 M NaAc, pH 5.2. The PCP fragments were recovered by centrifugation, washed in 70% EtOH, dried and resuspended in 20 microliters of restriction enzyme buffer (10 mM Tris-HCl, 10 mM MgCl2, 50 mM NaCl, 1 mM DTT). The fragments were digested with HindIII and XbaI, phenol and chloroform extracted, recovered by precipitation with 2 vols of 96% EtOH and 0.1 of 3 M NaAc, pH 5.2, and subcloned into HindIII/XbaI-cleaved pYES 2.0 vector.
  • Screening of cDNA Libraries and Characterization of the Positive Clones
  • cDNA libraries in S. cerevisiae or E. coli, constructed as described below, were screened by colony hybridization (Sambrook, 1989) using the corresponding random-primed (Feinberg and Vogelstein) 32P-labeled (>1×109 cpm/microgram) PCR products as probes. The hybridizations were carried out in 2×SSC (Sambrook, 1989), 5×Denhardt's solution (Sambrook, 1989), 0.5% (w/v) SDS, 100 micrograms/ml denatured salmon sperm DNA for 20 h at 65° C. followed by washes in 5×SSC at 25° C. (2×15 min), 2×SSC 0.5% SDS at 65° C. (30 min), 0.2×SSC 0.5% SDS at 65° C. (30 min) and finally in 5×SSC (2×15 min) at 25° C. The positive cDNA clones were characterized by sequencing the ends of the cDNA inserts with pYES 2.0 polylinker primers (Invitrogen, USA), and by determining the nucleotide sequence of the longest cDNA from both strands by the dideoxy chain termination method (Sanger et al.) using fluorescent labeled terminators. Qiagen purified plasmid DNA (Qiagen, USA) was sequenced with the Taq deoxy terminal cycle sequencing kit (Perkin Elmer, USA) and either pYES 2.0 polylinker primers (Invitrogen, USA) or synthetic oligonucleotide primers using an Applied Biosystems 373A automated sequencer according to the manufacturer's instructions. Analysis of the sequence data was performed according to Devereux et al.
  • Extraction of total RNA was performed with guanidinium thiocyanate followed by ultracentrifugation through a 5.7 M CsCl cushion, and isolation of poly(A)+ RNA was carried out by oligo(dT)-cellulose affinity chromatography using the procedures described in WO 94/14953.
  • cDNA Synthesis
  • Double-stranded cDNA was synthesized from 5 micrograms poly(A)+ RNA by the RNase H method (Gubler and Hoffman Gene 25:263-269 (1983), Sambrook et al. (1939) Molecular cloning: A laboratory manual, Cold Spring Harbor lab., Cold Spring Harbor, N.Y.) using the hair-pin modification developed by F. S. Hagen (pers. comm.). The poly(A)+ RNA (5 micrograms in 5 microliters of DEPC-treated water) was heated at 70° C. for 8 min. in a pre-siliconized, RNase-free Eppendorph tube, quenched on ice and combined in a final volume of 50 microliters with reverse transcriptase buffer (50 mM Tris-Cl, pH 8.3, 75 mM KCl, 3 mM MgCl2, 10 mM DTT, Bethesda Research Laboratories) containing 1 mM of dATP, dGTP and dTTP and 0.5 mM 5-methyl-dCTP (Pharmacia), 40 units human placental ribonuclease inhibitor (RNasin, Promega), 1.45 micrograms of oligo(dT)18-Not I primer (Pharmacia) and 1000 units SuperScript II RNase H reverse transcriptase (Bethesda Research Laboratories). First-strand cDNA was synthesized by incubating the reaction mixture at 45° C. for 1 hour. After synthesis, the mRNA:cDNA hybrid mixture was gelfiltrated through a MicroSpin S-400 HR (Pharmadia) spin column according to the manufacturer's instructions.
  • After the gelfiltration, the hybrids were diluted in 250 microliters second strand buffer (20 mM Tris-Cl, pH 7.4, 90 mM KCl, 4.6 mM MgCl2, 10 mM (NH4)2SO4, 0.16 mM βNAD+) containing 200 micro-M of each dNTP, 60 units E. coli DNA polymerase I (Pharmacia), 5.25 units RNase H (Promega) and 15 units E. coli DNA ligase (Boehringer Mannheim). Second strand cDNA synthesis was performed by incubating the reaction tube at 16° C. for 2 hours and additional 15 min. at 25° C. The reaction was stopped by addition of EDTA to a final concentration of 20 mM followed by phenol x's and chloroform extractions.
    • Mung Bean Nuclease Treatment
  • The double-stranded cDNA was precipitated at −20° C. for 12 hours by addition of 2 vols 96% EtOH, 0.2 vol 10 M NH4Ac, recovered by centrifugation, washed in 70% EtOH, dried and resuspended in 30 microliters Mung bean nuclease buffer (30 mM NaAc, pH 4.6, 300 mM NaCl, 1 mM ZnSO4, 0.35 mM DTT, 2% glycerol) containing 25 units Mung bean nuclease (Pharmacia). The single-stranded hair-pin DNA was clipped by incubating the reaction at 30° C. for 30 min., followed by addition of 70 microliters 10 mM Tris-Cl, pH 7.5, 1 mM EDTA, phenol extraction and precipitation with 2 vols of 96% EtOH and 0.1 vol 3 M NaAc, pH 5.2 on ice for 30 min.
  • Blunt-Ending with T4 DNA Polymerase
  • The double-stranded cDNAs were recovered by centrifugation and blunt-ended in 30 microliters T4 DNA polymerase buffer (20 mM Tris-acetate, pH 7.9, 10 mM MgAc, 50 mM KAc, 1 mM DTT) containing 0.5 mM of each dNTP and 5 units T4 DNA polymerase (New England Biolabs) by incubating the reaction mixture at 16° C. for 1 hour. The reaction was stopped by addition of EDTA to a final concentration of 20 mM, followed by phenol and chloroform extractions, and precipitation for 12 hours at −20° C. by adding 2 vols 96% EtOH and 0.1 vol 3 M NaAc pH 5.2.
    • Adaptor Ligation, Not I Digestion and Size Selection:
  • After the fill-in reaction the cDNAs were recovered by centrifugation, washed in 70% EtOH and dried. The cDNA pellet was resuspended in 25 microliters ligation buffer (30 mM Tris-Cl, pH 7.8, 10 mM MgCl2, 10 mM DTT, 0.5 mM ATP) containing 2.5 micrograms non-palindromic BstXI adaptors (invitrogen) and 30 units T4 ligase (Promega) and incubated at 15° C. for 12 hours. The reaction was stopped by heating at 65° C. for 20 min. and then cooling on ice for 5 min. The adapted cDNA was digested with Not I restriction enzyme by addition of 20 microliters water, 5 microliters 10× Not I restriction enzyme buffer (New England Biolabs) and 50 units Not I (New England Biolabs), followed by incubation for 2.5 hours at 37° C. The reaction was stopped by heating at 65° C. for 10 min. The cDNAs were size-fractionated by gel electrophoresis on a 0.8% SeaPlaque GTG low melting temperature agarose gel (FMC) in 1×TBE to separate unligated adaptors and small cDNAs. The cDNA was size-selected with a cut-off at 0.7 kb and rescued from the gel by use of beta-Agarase (New England Biolabs) according to the manufacturer's instructions and precipitated for 12 hours at −20° C. by adding 2 vols 96% EtOH and 0.1 vol 3 M NaAc pH 5.2.
    • Construction of Libraries
  • The directional, size-selected cDNA was recovered by centrifugation, washed in 70% EtOH, dried and resuspended in 30 microliters 10 mM Tris-Cl, pH 7.5, 1 mM EDTA. The cDNAs were desalted by gelfiltration through a MicroSpin S-300 HR (Pharmacia) spin column according to the manufacturers instructions. Three test ligations were carried out in 10 microliters ligation buffer (30 mM Tris-Cl, pH 7.8, 10 mM MgCl2, 10 mM DTT, 0.5 mM ATP) containing 5 microliters double-stranded cDNA (reaction tubes #1 and #2), 15 units T4 ligase (Promega) and 30 ng (tube #1), 40 ng (tube #2) and 40 ng (tube #3, the vector background control) of BstXI-NotI cleaved pYES 2.0 vector. The ligation reactions were performed by incubation at 16° C. for 12 hours, heating at 70° C. for 20 min. and addition of 10 microliters water to each tube. One microliter of each ligation mixture was electroporated into 40 microliters electrocompetent E. coli DH10B cells (Bethesda research Laboratories) as described (Sambrook et al. (1989) Molecular cloning: A laboratory manual, Cold Spring Harbor lab., Cold Spring Harbor, N.Y.). Using the optimal conditions a library was established in E. coli consisting of pools. Each pool was made by spreading transformed E. coli on LB+ampicillin agar plates giving 15.000-30.000 colonies/plate after incubation at 37° C. for 24 hours. 20 ml LB+ampicillin was added to the plate and the cells were suspended herein. The cell suspension was shaked in a 50 ml tube for 1 hour at 37° C. Plasmid DNA was isolated from the cells according to the manufacturer's instructions using QIAGEN plasmid kit and stored at −20° C.
  • One microliter aliquots of purified plasmid DNA (100 ng/microliter) from individual pools were transformed into S. cerevisiae W3124 by electroporation (Becker and Guarante, Methods Enzymol. 194: 182-187 (1991)) and the transformants were plated on SC agar containing 2% glucose and incubated at 30° C.
    • Identification of Positive Colonies
  • After 3-5 days of growth, the agar plates were replica plated onto a set of SC+galactose-uracil agar plates containing 0.1% AZCL HE cellulose. These plates were incubated for 3-7 days at 30° C. Endoglucanase positive colonies were identified as colonies surrounded by a blue halo.
  • Cells from enzyme-positive colonies were spread for single colony isolation on agar, and an enzyme-producing single colony was selected for each of the endoglucanase-producing colonies identified.
    • Characterization of Positive Clones
  • The positive clones were obtained as single colonies, the cDNA inserts were amplified directly from the yeast colony using biotinylated polylinker primers, purified by magnetic beads (Dynabead M-280, Dynal) system and characterized individually by sequencing the 5′-end of each cDNA clone using the chain-termination method (Sanger et al. (1977) Proc. Natl. Acad. Sci. U.S.A. 74: 5463-5467) and the Sequence system (United States Biochemical).
  • The nucleotide sequence was determined of the longest cDNA from both strands by the dideoxy chain termination method (Sanger et al.) using fluorescent labeled terminators. Plasmid DNA was rescued by transformation into E. coli as described below. Qiagen purified plasmid DNA (Qiagen, USA) was sequenced with the Taq deoxy terminal cycle sequencing kit (Perkin Elmer, USA) and either pYES 2.0 polylinker primers (Invitrogen, USA) or synthetic oligonucleotide primers using an Applied Biosystems 373A automated sequencer according to the manufacturer's instructions. Analysis of the sequence data was performed according to Devereux et at
  • Isolation of a cDNA Gene for Expression in Aspergillus
  • An endoglucanase-producing yeast colony was inoculated into 20 ml YPD broth in a 50 ml grass test tube. The tube was shaken for 2 days at 30° C. The cells were harvested by centrifugation for 10 min. at 3000 rpm.
  • DNA was isolated according to WO 94/14953 and dissolved in 50 microliters water. The DNA was transformed into E. coli by standard procedures. Plasmid DNA was isolated from E. coli using standard procedures, and analyzed by restriction enzyme analysis. The cDNA insert was excised using appropriate restriction enzymes and ligated into an Aspergillus expression vector.
  • Transformation of Aspergillus oryzae or Aspergillus Niger
  • Protoplasts may be prepared as described in WO 95/02043, p. 16, line 21-page 17, line 12, which is hereby incorporated by reference.
  • 100 microliters of protoplast suspension is mixed with 5-25 micrograms of the appropriate DNA in 10 microliters of STC (1.2 M sorbitol, 10 mM Tris-HCl, pH 7.5, 10 mM CaCl2. Protoplasts are mixed with p3SR2 (an A. nidulans amdS gene carrying plasmid). The mixture is left at room temperature for 25 minutes. 0.2 ml of 60% PEG 4000 (BDH 29576): 10 mM CaCl2 and 10 mM Tris-HCl, pH 7.5 is added and carefully mixed (twice) and finally 0.85 ml of the same solution is added and carefully mixed. The mixture is left at room temperature for 25 minutes, spun at 2500 g for 15 minutes and the pellet is resuspended in 2 ml of 1.2 M sorbitol. After one more sedimentation the protoplasts are spread on minimal plates (Cove, Biochem. Biophys. Acta 113: 51-56 (1966)) containing 1.0 M sucrose, pH 7.0, 10 mM acetamide as nitrogen source and 20 mM CsCl to inhibit background growth. After incubation for 4-7 days at 37° C. spores are picked and spread for single colonies. This procedure is repeated and spores of a single colony after the second resolution is stored as a defined transformant.
  • Test of A. oryzae Transformants
  • Each of the transformants were inoculated in 10 ml YPM and propagated. After 2-5 days of incubation at 37° C., 10 ml supernatant was removed. The endoglucanase activity was identified by AZCL HE cellulose as described above.
  • Hybridization conditions (to be used in evaluating property ii) of the DNA construct of the invention): Suitable conditions for determining hybridization between a nucleotide probe and a homologous DNA or RNA sequence involves presoaking of the filter containing the DNA fragments or RNA to hybridize in 5×SSC (standard saline citrate) for 10 min, and prehybridization of the filter in a solution of 5×SSC (Sambrook et al. 1989), 5×Denhardt's solution (Sambrook et al. 1989), 0.5% SDS and 100 micrograms/ml of denatured sonicated salmon sperm DNA (Sambrook et al. 1989), followed by hybridization in the same solution containing a random-primed (Feinberg, A. P. and Vogelstein, B., Anal, Biochem. 132:6-13 (1983)), 32P-dCTP-labeled (specific activity >1×109 cpm/microgram) probe for 12 hours at ca. 45° C. The filter is then washed two times for 30 minutes in 2×SSC, 0.5% SDS at preferably not higher than 50° C., more preferably not higher than 55° C., more preferably not higher than 60° C., more preferably not higher than 65° C., even more preferably not higher than 70° C., especially not higher than 75° C.
  • The nucleotide probe to be used in the hybridization is the DNA sequence corresponding to the endoglucanase encoding part of the DNA sequence of SEQ ID NO: 1, 7, 9, 11, 13, 15, 21 or 25, respectively, and/or the DNA sequence obtainable from the plasmid in S. cerevisiae, DSM 9770, DSM 10082, DSM 10080, DSM 10081, E. coli, DSM 10512, DSM 10511: DSM 10571 or DSM 10576, respectively.
    • Immunological Cross-Reactivity
  • Antibodies to be used in determining immunological cross-reactivity may be prepared by use of a purified cellulase. More specifically, antiserum against the cellulase of the invention may be raised by immunizing rabbits (or other rodents) according to the procedure described by N. Axelsen et al. in: A Manual of Quantitative Immunoelectrophoresis, Blackwell Scientific Publications, 1973, Chapter 23, or A. Johnstone and R. Thorpe, Immunochemistry in Practice, Blackwell Scientific Publications, 1982 (more specifically pp. 27-31). Purified immunoglobulins may be obtained from the antisera, for example by salt precipitation ((NH4)2 SO4), followed by dialysis and ion exchange chromatography, e.g., on DEAE-Sephadex. Immunochemical characterization of proteins may be 2 done either by Qutcherlony double-diffusion analysis (O. Ouchterlony in: Handbook of Experimental Immunology (D. M., Weir, Ed.), Blackwell Scientific Publications, 1967, pp. 655-706), by crossed immunoelectrophoresis (N. Axelsen et al., supra, Chapters 3 and 4), or by rocket immunoelectrophoresis (N. Axelsen et al., Chapter 2).
    • Media
  • YPD: 10 g yeast extract, 20 g peptone, H2O to 900 ml. Autoclaved, 100 ml 20% glucose (sterile filtered) added.
  • YPM: 10 g yeast extract, 20 g peptone, H2O to 900 ml. Autoclaved, 100 ml 20% maltodextrin (sterile filtered) added.
  • 10× Basal salt: 75 g yeast nitrogen base, 113 g succinic acid, 68 g NaOH, H2O ad 1000 ml, sterile filtered.
  • SC-URA: 100 ml 10× Basal salt, 28 ml 20% casamino acids without vitamins, 10 ml 1% tryptophan, H2O ad 900 ml, autoclaved, 3.6 ml 5% threonine and 100 ml 20% glucose or 20% galactose added.
  • SC-URA agar: SC-URA, 20 g/l agar added.
  • PD agar: 39 g potato dextrose agar, DIFCO 0013: add deionized water up to 1000 ml; autoclave (121° C. for 15-20 min).
  • PC agar: Potatoes and carrots (grinded, 20 g of each) and water, added up to 1000 ml, are boiled for 1 hr; agar (20 g/l of Merck 1614); autoclave (121° C. for 20 min)
  • PC liquid broth: as PC agar but without the Agar
  • PD liquid broth: 24 g potato dextrose broth, Difco 0549, deionized water up to 1000 ml; autoclave (121° C. for 15-20 min)
  • PC and PD liquid broth with cellulose: add 30 g Solcafloc (Dicacel available from Dicalite-Europe-Nord, 9000 Gent, Belgium) per 1000 ml
  • PB-9 liquid broth: 12 g Rofec (Roquette 101-0441) and 24 g glucose are added to 1000 ml water; pH is adjusted to 5.5; 5 ml mineral oil and 5 g CaCO3 are added per 1000 ml. Autoclave (121° C. for 40 min)
  • YPG liquid broth: 4 g yeast extract (Difco 0127), 1 g KH2PO4 (Merck4873), 0.5 g MgSO4.7H2O Merck 5886, 15 g Dextrose, Roquette 101-0441, 0.1 ml Pluronic (101-3088); deionized water up to 1000 ml; autoclave (20 min at 121° C.)
  • Dilute salt solution (DS): Make up two stock solutions:
  • P-stock: 13.61 g KH2PO4; 13.21 g (NH4)2PO4, 17.42 g KH2PO4; deionized water up to 100 ml Ca/Mg stock: 7.35 g CaCl2, 2H2O, 10.7 g MgCl2, 6H2O, deionized water up to 100 ml; pH adjusted to 7.0; autoclaving (121° C.; 20 min)
  • Mix 0.5 ml P-stock with 0.1 ml Ca/Mg stock
  • add deionized water up to 1000 ml
  • AZCL HE cellulose (Megazyme, Australia).
    • Uses
  • During washing and wearing, dyestuff from dyed fabrics or garment will conventionally bleed from the fabric which then looks faded and worn. Removal of surface fibers from the fabric will partly restore the original colours and looks of the fabric. By the term “colour clarification”, as used herein, is meant the partly restoration of the initial colours of fabric or garment throughout multiple washing cycles.
  • The term “de-pilling” denotes removing of pills from the fabric surface.
  • The term “soaking liquor” denotes an aqueous liquor in which laundry may be immersed prior to being subjected to a conventional washing process. The soaking liquor may contain one or more ingredients conventionally used in a washing or laundering process.
  • The term “washing liquor” denotes an aqueous liquor in which laundry is subjected to a washing process, i.e., usually a combined chemical and mechanical action either manually or in a washing machine. Conventionally, the washing liquor is an aqueous solution of a powder or liquid detergent composition.
  • The term “rinsing liquor” denotes an aqueous liquor in which laundry is immersed and treated, conventionally immediately after being subjected to a washing process, in order to rinse the laundry, i.e., essentially remove the detergent solution from the laundry. The rinsing liquor may contain a fabric conditioning or softening composition.
  • The laundry subjected to the method of the present invention may be conventional washable laundry. Preferably, the major part of the laundry is sewn or unsewn fabrics, including knits, wovens, denims, yarns, and toweling, made from cotton, cotton blends or natural or manmade cellulosics (e.g., originating from xylan-containing cellulose fibers such as from wood pulp) or blends thereof. Examples of blends are blends of cotton or rayon/viscose with one or more companion material such as wool, synthetic fibers (e.g., polyamide fibers, acrylic fibers, polyester fibers, polyvinyl alcohol fibers, polyvinyl chloride fibers, polyvinylidene chloride fibers, polyurethane fibers, polyurea fibers, aramid fibers), and cellulose-containing fibers (e.g., rayon/viscose, ramie, flax/linen, jute, cellulose acetate fibers, lyocell).
    • Detergent Compositions
  • According to one aspect of the present invention, the present endoglucanases may typically be components of a detergent composition. As such, they may be included in the detergent composition in the form of a non-dusting granulate, a stabilized liquid, or protected enzymes. Non-dusting granulates may be produced, e.g., as disclosed in U.S. Pat. Nos. 4,106,991 and 4,661,452 (both to to Novo Industri A/S) and may optionally be coated by methods known in the art, Examples of waxy coating materials are poly(ethylene oxide) products (polyethyleneglycol, PEG) with mean molecular weights of 1000 to 20000; ethoxylated nonylphenols having from 16 to 50 ethylene oxide units; ethoxylated fatty alcohols in which the alcohol contains from 12 to 20 carbon atoms and in which there are 15 to 80 ethylene oxide units; fatty alcohols; fatty acids; and mono and di- and triglycerides of fatty acids, Examples of film-forming coating materials suitable for application by fluid bed techniques are given in patent GB 1483591. Liquid enzyme preparations may, for instance, be stabilized by adding a polyol such as propylene glycol, a sugar or sugar alcohol, lactic acid or boric acid according to established methods. Other enzyme stabilizers are welt known in the art. Protected enzymes may be prepared according to the method disclosed in EP 238,216.
  • The detergent composition of the invention may be in any convenient form, e.g., as powder, granules, paste or liquid. A liquid detergent may be aqueous, typically containing up to 70% water and 0-30% organic solvent, or nonaqueous.
  • The detergent composition comprises one or more surfactants, each of which may be anionic, nonionic, cationic, or zwitterionic. The detergent will usually contain 0-50% of anionic surfactant such as linear alkylbenzenesulfonate (LAS), alpha-olefinsulfonate (AOS), alkyl sulfate (fatty alcohol sulfate) (AS), alcohol ethoxysulfate (AEOS or AES), secondary alkanesulfonates (SAS), alpha-sulfo fatty acid methyl esters, alkyl- or alkenylsuccinic acid, or soap. It may also contain 0-40% of nonionic surfactant such as alcohol ethoxylate (AEO or AE), carboxylated alcohol ethoxylates, nonylphenol ethoxylate, alkylpolyglycoside, alkyldimethylamine oxide, ethoxylated fatty acid monoethanolamide, fatty acid monoethanolamide, or polyhydroxy alkyl fatty acid amide (e.g. as described in WO 92/06154).
  • The detergent composition may additionally comprise one or more other enzymes such as amylase, lipase, cutinase, protease, peroxidase, and oxidase, e.g., laccase.
  • The detergent may contain 1-65% of a detergent builder or complexing agent such as zeolite, diphosphate, triphosphate, phosphonate, citrate, nitrilotriacetic acid (NTA), ethylenediaminetetraacetic acid (EDTA), diethylenetriaminepentaacetic acid (DTMPA), alkyl- or alkenylsuccinic acid, soluble silicates or layered silicates (eg., SKS-6 from Hoechst). The detergent may also be inbuilt, i.e., essentially free of detergent builder.
  • The detergent may comprise one or more polymers. Examples are carboxymethylcellulose (CMC), poly(vinylpyrrolidone) (PVP), polyethyleneglycol (PEG), poly(vinyl alcohol) (PVA), polycarboxylates such as polyacrylates, maleic/acrylic acid copolymers and lauryl methacrylate/acrylic acid copolymers.
  • The detergent may contain a bleaching system which may comprise a H2O2 source such as perborate or percarbonate which may be combined with a peracid-forming bleach activator such as tetraacetylethylenediamine (TAED) or Nonanoyloxybenzenesulfonate (NOBS). Alternatively, the bleaching system may comprise peroxyacids of, erg., the amide, imide, or sulfone type.
  • The enzymes of the detergent composition of the invention may be stabilized using conventional stabilizing agents, e.g., a polyol such as propylene glycol or glycerol, a sugar or sugar alcohol, lactic acid, boric acid, or a boric acid derivative such as, e.g., an aromatic borate ester, and the composition may be formulated as described in, e.g., WO 92/19709 and WO 92/19708.
  • The detergent may also contain other conventional detergent ingredients such as, e.g., fabric conditioners including clays, foam boosters, suds suppressors, anti-corrosion agents, soil-suspending agents, anti-soil-redeposition agents, dyes, bactericides, optical brighteners, or perfume.
  • The pH (measured in aqueous solution at use concentration) will usually be neutral or alkaline, e.g., in the range of 7-11.
  • Particular forms of detergent compositions within the scope of the invention include:
  • 1) A detergent composition formulated as a granulate having a bulk density of at least 600 g/l comprising
  •
    Linear alkylbenzenesulfonate (calculated as acid)  7-12%
    Alcohol ethoxysulfate (e.g., C12-18 alcohol, 1-2 EO) 1-4%
    or alkyl sulfate (e.g., C16-18)
    Alcohol ethoxylate (e.g., C14-15 alcohol, 7 EO) 5-9%
    Sodium carbonate (as Na2CO3) 14-20%
    Soluble silicate (as Na2O, 2SiO2) 2-6%
    Zeolite (as NaA1SiO4) 15-22%
    Sodium sulfate (as Na2SO4) 0-6%
    Sodium citrate/citric acid (as C6H5Na3O7/C6H8O7)  0-15%
    Sodium perborate (as NaBO3•H2O) 11-18%
    TAED 2-6%
    Carboxymethylcellulose 0-2%
    Polymers (e.g., maleic/acrylic acid copolymer, PVP, 0-3%
    PEG)
    Enzymes (calculated as pure enzyme protein) 0.0001-0.1%  
    Minor ingredients (e.g., suds suppressors, perfume, 0-5%
    optical brightener, photobleach)

    2) A detergent composition formulated as a granulate having a bulk density of at least 600 g/l comprising
  • Linear alkylbenzenesulfonate (calculated as acid)  6-11%
    Alcohol ethoxysulfate (e.g., C12-18 alcohol, 1-2 EO or 1-3%
    alkyl sulfate (e.g., C16-18)
    Alcohol ethoxylate (e.g., C14-15 alcohol, 7 EO) 5-9%
    Sodium carbonate (as Na2CO3) 15-21%
    Soluble silicate (as Na2O, 2SiO2) 1-4%
    Zeolite (as NaA1SiO4) 24-34%
    Sodium sulfate (as Na2SO4)  4-10%
    Sodium citrate/citric acid (as C6H5Na3O7/C6H8O7)  0-15%
    Carboxymethylcellulose 0-2%
    Polymers (e.g., maleic/acrylic acid copolymer, PVP, 1-6%
    PEG)
    Enzymes (calculated as pure enzyme protein) 0.0001-0.1%  
    Minor ingredients (e.g., suds suppressors, perfume) 0-5%

    3) A detergent composition formulated as a granulate having a bulk density of at least 600 g/l comprising
  • Linear alkylbenzenesulfonate (calculated as acid) 5-9%
    Alcohol ethoxylate (e.g., C12-15 alcohol, 7 EO)  7-14%
    Soap as fatty acid (e.g., C16-22 fatty acid) 1-3%
    Sodium carbonate (as Na2CO3) 10-17%
    Soluble silicate (as Na2O, 2SiO2) 3-9%
    Zeolite (as NaA1SiO4) 23-33%
    Sodium sulfate (as Na2SO4) 0-4%
    Sodium perborate (as NaBO3•H2O)  8-16%
    TAED 2-8%
    Phosphonate (e.g., EDTMPA) 0-1%
    Carboxymethylcellulose 0-2%
    Polymers (e.g., maleic/acrylic acid copolymer, PVP, 0-3%
    PEG)
    Enzymes (calculated as pure enzyme protein) 0.0001-0.1%  
    Minor ingredients (e.g., suds suppressors, perfume, 0-5%
    optical brightener)

    4) A detergent composition formulated as a granulate having a bulk density of at least 600 g/l comprising
  • Linear alkylbenzenesulfonate (calculated as acid)  8-12%
    Alcohol ethoxylate (e.g., C12-15 alcohol, 7 EO) 10-25%
    Sodium carbonate (as Na2CO3) 14-22%
    Soluble silicate (as Na2O, 2SiO2) 1-5%
    Zeolite (as NaA1SiO4) 25-35%
    Sodium sulfate (as Na2SO4)  0-10%
    Carboxymethylcellulose 0-2%
    Polymers (e.g., maleic/acrylic acid copolymer, PVP, 1-3%
    PEG)
    Enzymes (calculated as pure enzyme protein) 0.0001-0.1%  
    Minor ingredients (e.g., suds suppressors, perfume) 0-5%

    5) An aqueous liquid detergent composition comprising
  • Linear alkylbenzenesulfonate (calculated as acid) 15-21%
    Alcohol ethoxylate (e.g., C12-15 alcohol, 7 EO or 12-18%
    C12-15 alcohol, 5 EO)
    Soap as fatty acid (e.g., oleic acid)  3-13%
    Alkenylsuccinic acid (C12-14)  0-13%
    Aminoethanol  8-18%
    Citric acid 2-8%
    Phosphonate 0-3%
    Polymers (e.g., PVP, PEG) 0-3%
    Borate (as B4O7) 0-2%
    Ethanol 0-3%
    Propylene glycol  8-14%
    Enzymes (calculated as pure enzyme protein) 0.0001-0.1%  
    Minor ingredients (e.g., dispersants, suds suppressors, 0-5%
    perfume, optical brightener)

    6) An aqueous structured liquid detergent composition comprising
  • Linear alkylbenzenesulfonate (calculated as acid) 15-21%
    Alcohol ethoxylate (e.g., C12-15 alcohol, 7 EO, or 3-9%
    C12-15alcohol, 5 EO)
    Soap as fatty acid (e.g., oleic acid)  3-10%
    Zeolite (as NaA1SiO4) 14-22%
    Potassium citrate  9-18%
    Borate (as B4O7) 0-2%
    Carboxymethylcellulose 0-2%
    Polymers (e.g., PEG, PVP) 0-3%
    Anchoring polymers such as, e.g., lauryl 0-3%
    methacrylate/acrylic acid copolymer;
    molar ratio 25:1; MW 3800
    Glycerol 0-5%
    Enzymes (calculated as pure enzyme protein) 0.0001-0.1%  
    Minor ingredients (e.g., dispersants, suds suppressors, 0-5%
    perfume, optical brighteners)

    7) A detergent composition formulated as a granulate having a bulk density of at least 600 g/l comprising
  • Fatty alcohol sulfate  5-10%
    Ethoxylated fatty acid monoethanolamide 3-9%
    Soap as fatty acid 0-3%
    Sodium carbonate (as Na2CO3)  5-10%
    Soluble silicate (as Na2O, 2SiO2) 1-4%
    Zeolite (as NaA1SiO4) 20-40%
    Sodium sulfate (as Na2SO4) 2-8%
    Sodium perborate (as NaBO3•H2O) 12-18%
    TAED 2-7%
    Polymers (e.g., maleic/acrylic acid copolymer, PEG) 1-5%
    Enzymes (calculated as pure enzyme protein) 0.0001-0.1%  
    Minor ingredients (e.g., optical brightener, suds 0-5%
    suppressors, perfume)

    8) A detergent composition formulated as a granulate comprising
  • Linear alkylbenzenesulfonate (calculated as acid)  8-14%
    Ethoxylated fatty acid monoethanolamide  5-11%
    Soap as fatty acid 0-3%
    Sodium carbonate (as Na2CO3)  4-10%
    Soluble silicate (as Na2O, 2SiO2) 1-4%
    Zeolite (as NaA1SiO4) 30-50%
    Sodium sulfate (as Na2SO4)  3-11%
    Sodium citrate (as C6H5Na3O7)  5-12%
    Polymers (e.g., PVP, maleic/acrylic acid copolymer, 1-5%
    PEG)
    Enzymes (calculated as pure enzyme protein) 0.0001-0.1%  
    Minor ingredients (e.g., suds suppressors, perfume) 0-5%

    9) A detergent composition formulated as a granulate comprising
  • Linear alkylbenzenesulfonate (calculated as acid)  6-12%
    Nonionic surfactant 1-4%
    Soap as fatty acid 2-6%
    Sodium carbonate (as Na2CO3) 14-22%
    Zeolite (as NaA1SiO4) 18-32%
    Sodium sulfate (as Na2SO4)  5-20%
    Sodium citrate (as C6H5Na3O7) 3-8%
    Sodium perborate (as NaBO3•H2O) 4-9%
    Bleach activator (e.g., NOBS or TAED) 1-5%
    Carboxymethylcellulose 0-2%
    Polymers (e.g., polycarboxylate or PEG) 1-5%
    Enzymes (calculated as pure enzyme protein) 0.0001-0.1%  
    Minor ingredients (e.g., optical brightener, perfume) 0-5%

    10) An aqueous liquid detergent composition comprising
  • Linear alkylbenzenesulfonate (calculated as acid) 15-23%
    Alcohol ethoxysulfate (e.g., C12-15 alcohol, 2-3 EO)  8-15%
    Alcohol ethoxylate (e.g., C12-15 alcohol, 7 EO, or 3-9%
    C12-15alcohol, 5 EO)
    Soap as fatty acid (e.g., lauric acid) 0-3%
    Aminoethanol 1-5%
    Sodium citrate  5-10%
    Hydrotrope (e.g., sodium toluensulfonate) 2-6%
    Borate (as B4O7) 0-2%
    Carboxymethylcellulose 0-1%
    Ethanol 1-3%
    Propylene glycol 2-5%
    Enzymes (calculated as pure enzyme protein) 0.0001-0.1%  
    Minor ingredients (e.g., polymers, dispersants, 0-5%
    perfume, optical brighteners)

    11) An aqueous liquid detergent composition comprising
  • Linear alkylbenzenesulfonate (calculated as acid) 20-32%
    Alcohol ethoxylate (e.g., C12-15 alcohol, 7 EO, or  6-12%
    C12-15 alcohol, 5 EO)
    Aminoethanol 2-6%
    Citric acid  8-14%
    Borate (as B4O7) 1-3%
    Polymer (e.g., maleic/acrylic acid copolymer, 0-3%
    anchoring polymer such as, e.g., lauryl
    methacrylate-/acrylic acid copolymer)
    Glycerol 3-8%
    Enzymes (calculated as pure enzyme protein) 0.0001-0.1%  
    Minor ingredients (e.g., hydrotropes, dispersants, 0-5%
    perfume, optical brighteners)

    12) A detergent composition formulated as a granulate having a bulk density of at least 600 gal comprising
  • Anionic surfactant (linear alkylbenzenesulfonate, 25-40%
    alkyl sulfate, alpha-olefinsulfonate, alpha-sulfo fatty
    acid methyl esters, alkanesulfonates, soap)
    Nonionic surfactant (e.g., alcohol ethoxylate)  1-10%
    Sodium carbonate (as Na2CO3)  8-25%
    Soluble silicates (as Na2O, 2SiO2)  5-15%
    Sodium sulfate (as Na2SO4) 0-5%
    Zeolite (as NaA1SiO4) 15-28%
    Sodium perborate (as NaBO3•4H2O)  0-20%
    Bleach activator (TAED or NOBS) 0-5%
    Enzymes (calculated as pure enzyme protein) 0.0001-0.1%  
    Minor ingredients (e.g., perfume, optical brighteners) 0-3%

    13) Detergent formulations as described in 1)-12) wherein all or part of the linear alkylbenzenesulfonate is replaced by (C12-C18) alkyl sulfate. 14) A detergent composition formulated as a granulate having a bulk density of at least 600 g/l comprising
  • (C12-C18) alkyl sulfate  9-15%
    Alcohol ethoxylate 3-6%
    Polyhydroxy alkyl fatty acid amide 1-5%
    Zeolite (as NaA1SiO4) 10-20%
    Layered disilicate (e.g., SK56 from Hoechst) 10-20%
    Sodium carbonate (as Na2CO3)  3-12%
    Soluble silicate (as Na2O, 2SiO2) 0-6%
    Sodium citrate 4-8%
    Sodium percarbonate 13-22%
    TAED 3-8%
    Polymers (e.g., polycarboxylates and PVP = 0-5%
    Enzymes (calculated as pure enzyme protein) 0.0001-0.1%  
    Minor ingredients (e.g., optical brightener, photo 0-5%
    bleach, perfume, suds suppressors)

    15) A detergent composition formulated as a granulate having a bulk density of at least 600 g/l comprising
  • (C12-C18) alkyl sulfate 4-8%
    Alcohol ethoxylate 11-15%
    Soap 1-4%
    Zeolite MAP or zeolite A 35-45%
    Sodium carbonate (as Na2CO3) 2-8%
    Soluble silicate (as Na2O, 2SiO2) 0-4%
    Sodium percarbonate 13-22%
    TAED 1-8%
    Carboxymethyl cellulose 0-3%
    Polymers (e.g., polycarboxylates and PVP) 0-3%
    Enzymes (calculated as pure enzyme protein) 0.0001-0.1%  
    Minor ingredients (e.g., optical brightener, 0-3%
    phosphonate, perfume)

    16) Detergent formulations as described in 1)-15) which contain a stabilized or encapsulated peracid, either as an additional component or as a substitute for already specified bleach systems.
    17) Detergent compositions as described in 1), 3), 7), 9) and 12) wherein perborate is replaced by percarbonate.
    18) Detergent compositions as described in 1), 3), 7), 9), 12), 14) and 15) which additionally contain a manganese catalyst. The manganese catalyst may, e., be one of the compounds described in “Efficient manganese catalysts for low-temperature bleaching”, Nature 369, 1994, pp. 637-639.
    19) Detergent composition formulated as a nonaqueous detergent liquid comprising a liquid nonionic surfactant such as, e.g., linear alkoxylated primary alcohol, a builder system (e.g., phosphate), enzyme and alkali. The detergent may also comprise anionic surfactant and/or a bleach system.
  • The endoglucanase may be incorporated in concentrations conventionally employed in detergents. It is at present contemplated that, in the laundry composition of the invention, the cellulase may be added in an amount corresponding to 0.0001-10 mg (calculated as pure enzyme protein) of cellulase per liter of wash liquor.
  • According to yet another aspect of the present invention, endoglucanase may typically be a component of a fabric conditioning or softener composition. Examples of conventional softener compositions are disclosed in, e.g., EP 0 233 910.
    • Textile Applications
  • In another embodiment, the present invention relates to use of the endoglucanase of the invention in the biopolishing process. Bio-Polishing is a specific treatment of the yarn surface which improves fabric quality with respect to handle and appearance without loss of fabric wettability. The most important effects of Bio-Polishing can be characterized by less fuzz and pilling, increased gloss/luster, improved fabric handle, increased durable softness and altered water absorbency. Bio-Polishing usually takes place in the wet processing of the manufacture of knitted and woven fabrics. Wet processing comprises such steps as, e.g., desizing, scouring, bleaching, washing, dying/printing and finishing. During each of these steps, the fabric is more or less subjected to mechanical action. In general, after the textiles have been knitted or woven, the fabric proceeds to a desizing stage, followed by a scouring stage, etc. Desizing is the act of removing size from textiles. Prior to weaving on mechanical looms, warp yarns are often coated with size starch or starch derivatives in order to increase their tensile strength. After weaving, the size coating must be removed before further processing the fabric in order to ensure a homogeneous and wash-proof result. It is known that in order to achieve the effects of Bio-Polishing, a combination of cellulytic and mechanical action is required. It is also known that “super-softness” is achievable when the treatment with a cellulase is combined with a conventional treatment with softening agents. It is contemplated that use of the endoglucanase of the invention for bio-polishing of cellulosic fabrics is advantageous, e.g., a more thorough polishing can be achieved. Bio-polishing may be obtained by applying the method described, e.g., in WO 93/20278.
    • Stone-washing
  • It is known to provide a “stone-washed” look (localized abrasion of the colour) in dyed fabric, to especially in denim fabric or jeans, either by washing the denim or jeans made from such fabric in the presence of pumice stones to provide the desired localized lightening of the colour of the fabric or by treating the fabric enzymatically, in particular with cellulytic enzymes. The treatment with an endoglucanase of the present invention may be carried out either alone such as disclosed in U.S. Pat. No. 4,832,864, together with a smaller amount of pumice than required in the traditional process, or together with perlite such as disclosed in WO 95/09225.
    • Pulp and Paper Applications
  • In the papermaking pulp industry, the endoglucanase of the present invention may be applied advantageously, e.g., as follows:
      • For debarking; pretreatment with the endoglucanase may degrade the cambium layer prior to debarking in mechanical drums resulting in advantageous energy savings.
      • For defibrillation: treatment of a material containing cellulosic fibers with the endoglucanase prior to refining or beating may result in reduction of the energy consumption due to the hydrolyzing effect of the cellulase on the interfiber surfaces. Use of the endoglucanase may result in improved energy savings as compared to the use of known enzymes, since it is believed that the enzyme composition of the invention may possess a higher ability to penetrate fiber waits.
      • For fiber modification, i.e., improvement of fiber properties where partial hydrolysis across the fiber watt is needed which requires deeper penetrating enzymes (eg., in order to make coarse fibers more flexible). Deep treatment of fibers has so far not been possible for high yield pulps, e.g., mechanical pulps or mixtures of recycled pulps. This has been ascribed to the nature of the fiber watt structure that prevents the passage of enzyme molecules due to physical restriction of the pore matrix of the fiber wall, it is contemplated that the present endoglucanase is capable of penetrating into the fiber wall,
      • For drainage improvement. The drainability of papermaking pulps may be improved by treatment of the pulp with hydrolyzing enzymes, e.g., cellulases. Use of the present endoglucanase may be more effective, e.g., result in a higher degree of loosening bundles of strongly hydrated micro-fibrils in the fines fraction (consisting of fiber debris) that limits the rate of drainage by blocking hollow spaces between fibers and in the wore mesh of the paper machine. The Canadian standard freeness (CSF) increases and the Schopper-Riegler drainage index decreases when pulp in subjected to cellulase treatment, see, e.g., U.S. Pat. No. 4,923,565; TAPPI T227, SCANC 19:65 ence.
      • For inter fiber bonding. Hydrolytic enzymes are applied in the manufacture of papermaking to pulps for improving the interfiber bonding. The enzymes rinse the fiber surfaces for impurities, eg., cellulosic debris, thus enhancing the area of exposed cellulose with attachment to the fiber wall, thus improving the fiber-to-fiber hydrogen binding capacity. This process is also referred to as dehornification. Paper and board produced with a cellulase containing enzyme preparation may have an improved strength or a reduced grammage, a smoother surface and an improved printability.
      • For enzymatic deinking. Partial hydrolysis of recycled paper during or upon pulping by use of hydrolyzing enzymes such as cellulases are known to facilitate the removal and agglomeration of ink particles. Use of the present endoglucanase may give a more effective loosening of ink from the surface structure due to a better penetration of the enzyme molecules into the fibrillar matrix of the fiber wall, thus softening the surface whereby ink particles are effectively loosened. The agglomeration of loosened ink particles are also improved, due to a more efficient hydrolysis of cellulosic fragments found attached to ink particles originating from the fibers.
  • The treatment of lignocellulosic pulp may, e.g., be performed as described in WO 91/14819, WO 91/14822, WO 92/17573 and WO 92/18688.
    • Degradation of Plant Material
  • In yet another embodiment, the present invention relates to use of the endoglucanase and/or enzyme preparation according to the invention for degradation of plant material, eg., cell walls.
  • It is contemplated that the novel endoglucanase and/or enzyme preparation of the invention is useful in the preparation of wine, fruit or vegetable juice in order to increase yield, Endoglucanases according to the invention may also be applied for enzymatic hydrolysis of various plant cell-wall derived materials or waste materials, e.g., agricultural residues such as wheat-straw, corn cobs, whole corn plants, nut shells, grass, vegetable hulls, bean hulls, spent grains, sugar beet pulp, and the like. The plant material may be degraded in order to improve different kinds of processing, facilitate purification or extraction of other components tike purification of beta-glucan or beta-glucan oligomers from cereals, improve the feed value, decrease the water binding capacity, improve the degradability in waste water plants, improve the conversion of, eg., grass and corn to ensilage, etc.
  • The following examples illustrate the invention.
    • EXAMPLE 1
  • Cellulytic enzymes from 4 fungi, belonging to 3 families under two orders within the Ascomycetes were detected by expression cloning; corresponding DNA sequences were determined; the enzymes heterologously expressed, and produced by liquid fermentation, characterized and demonstrated to give good performance in colour clarification assays.
  • Isolate CBS 117.65, CBS 478.94, NRRL 8126, and ATCC 10523 were grown in shake flask cultures on cellulose enriched potato dextrose broth, incubated for 5 days at 26° C. (shaking conditions, 150 rpm).
  • A. Cloning and Expression of an Endoglucanase from Myceliophthora thermophila, Acremonium sp., and Thielavia terrestris and Volutella colletotrichoides
  • mRNA was isolated from Myceliophthora thermophila, Acremonium sp., Thielavia terrestris and Volutella colletotrichoides, respectively, grown in a cellulose-containing fermentation medium with agitation to ensure sufficient aeration. Mycelia were harvested after 3-5 days' growth, immediately frozen in liquid nitrogen and stored at −80° C. Libraries from Myceliophthora thermophila, Acremonium sp., Thielavia terrestris and Volutella colletotrichoides, respectively, each consisting of approx. 106 individual clones were constructed in E. coli as described with a vector background of 1%.
  • Plasmid DNA from some of the pools from each library was transformed into yeast, and 50-100 plates containing 250-400 yeast colonies were obtained from each pool.
  • Endoglucanase-positive colonies were identified and isolated on SC-agar plates with the AZCL HE cellulose assay. cDNA inserts were amplified directly from the yeast colonies and characterized as described in the Materials and Methods section above.
  • The DNA sequence of the cDNA encoding the endoglucanase from Myceliophthora thermophila is SEQ ID NO: 1 and the corresponding amino acid sequence is SEQ ID NO: 2. The cDNA is obtainable from the plasmid in DSM 9770.
  • The DNA sequence of the cDNA encoding the endoglucanase from Acremonium sp. is SEQ ID NO: 7 and the corresponding amino acid sequence is SEQ ID NO: 8. The cDNA is obtainable from the plasmid in DSM 10032.
  • The DNA sequence of the cDNA encoding the endoglucanase from Thielavia terrestris is SEQ ID NO: 11 and the corresponding amino acid sequence is SEQ ID NO: 12. The cDNA is obtainable from the plasmid in DSM 10081.
  • The DNA sequence of the cDNA encoding the endoglucanase from Volutella colletotrichoides is SEQ ID NO: 21 and the corresponding amino acid sequence is SEQ ID NO: 22. The cDNA is obtainable from the plasmid in DSM 10571.
  • Total DNA was isolated from a yeast colony and plasmid DNA was rescued by transformation of E. coli as described above. In order to express the endoglucanases in Aspergillus, the DNA was digested with appropriate restriction enzymes, size fractionated on gel, and a fragment corresponding to the endoglucanase gene from Myceliophthora thermophila, Acremonium sp., Thielavia terrestris and Volutella colletotrichoides, respectively, was purified. The genes were subsequently ligated to pHD414, digested with appropriate restriction enzymes, resulting in the plasmids pA2C193, pA2C357, pA2C385 and pA2C488, respectively.
  • After amplification of the DNA in E. coli the plasmids were transformed into Aspergillus oryzae as described above,
  • Test of A. oryzae Transformants
  • Each of the transformants were tested for endoglucanase activity as described above. Some of the transformants had endoglucanase activity which was significantly larger than the Aspergillus oryzae background. This demonstrates efficient expression of the endoglucanases in Aspergillus oryzae. The transformants with the highest endoglucanase activity were selected and inoculated in a 500 ml shake flask with YPM media. After 3-5 days of fermentation with sufficient agitation to ensure good aeration, the culture broth was centrifuged for 10 minutes at 2000 g and the supernatant recovered.
  • B. Determination of Endoglucanase Activity The cellulytic activity of the endoglucanase may be determined relative to an analytical standard and expressed in the unit S-CEVU.
  • Cellulytic enzymes hydrolyze OMC, thereby decreasing the viscosity of the incubation mixture. The resulting reduction in viscosity may be determined by a vibration viscometer (e.g., MIVI 3000 from Sofraser, France).
  • Determination of the cellulytic activity, measured in terms of S-CEVU, may be determined according to the analysis method AF 301.1 which is available from the Applicant upon request.
  • The S-CEVU assay quantifies the amount of catalytic activity present in the sample by measuring the ability of the sample to reduce the viscosity of a solution of carboxymethylcellulose (CMC). The assay is carried out at 40° C., pH 7.5 using a relative enzyme standard for reducing the viscosity of the CMC substrate.
  • Assay for determination of endoglucanase activity in terms of SAVI units using phosphoric-acid swollen cellulose (PASC):
  • Definition: 1 SAVI-U is the amount of enzyme which forms an amount of reducing carbohydrates equivalent to 1 micro-mol of glucose per minute.
    • Assay Condition:
  • Enzyme solution, 0.5 ml
  • 4 g/l PASC in 0.1 M Buffer: 2.0 ml
  • 20 min, 40° C.
    • Sensitivity:
  • Max 0.1 SAVIU/ml=approx. 1 S-CEVU/ml (CMC viscosity)
  • Min 0.01 SAVIU/ml=approx. 0.1 S-CEVU/ml
    • Determination of Formation of Reducing Sugars:
  • The reducing groups assay was performed according to Lever, M. A new reaction for colormetric determination of carbohydrates. Anal. Biochem. 1972. Vol 47 (273-279). Reagent mixture was prepared by mixing 1.5 gram p-hydroxybenzoic-acid hydrazide (PHBAH) with 5 gram sodium tartrate in 100 ml 2% sodium hydroxide.
    • Substrate:
  • PASC stock solution was prepared the following way using ice cold acetone and phosphoric acid. 5 gram of cellulose (Avicel®) was moistened with water, and 150 ml ice cold 85% ortho-phosphoric acid was added. The mixture was placed in ice bath under slow stirring for 1 hr. Then 100 ml ice cold acetone was added with stirring. The slurry was transferred to a Buchner filter with pyrex sintered disc number 3 and then washed three times with 100 ml ice cold acetone, and sucked as dry as possible after each wash. Finally, the filter cake was washed twice with 500 ml water, sucked as dry as possible after each wash The PASC was mixed with deionized water to a total volume of 300 ml, blended to homogeneity (using the Ultra Turrax Homogenizer) and stored in refrigerator (up to one month).
  • Substrate equilibration with buffer: 20 gram phosphoric acid swollen cellulose PASC stock solution was centrifuged for 20 min at 5000 rpm., the supernatant was poured of; the sediment was resuspended in 30 ml of buffer and centrifuged for 20 min. at 5000 rpm., the supernatant was poured of, and the sediment was resuspended in buffer to a total of 60 g corresponding to a substrate concentration of 5 g cellulose/liter.
  • Buffer for pH 8.5 determination: 0.1 M Barbital,
    Buffer for pH 10 determination: 0.1 M Glycine.
    • Procedure: A. Dilution of Enzyme Samples
  • The enzyme solution is diluted in the same buffer as the substrate.
    • 2. Enzyme Reaction
  • The substrate in buffer solution is preheated for 5 min. at 40° C. (2 ml). Then the enzyme solution (diluted to between 0.2 and 1 S-CEVU/ml) 0.5 ml is added and mixed for 5 sec. Enzymes blanks are obtained by adding the stop reagent before enzyme solution. Incubate for 20 min. at 40° C., The reaction is stopped by adding 0.5 ml 2% NaOH solution and mixing for 5 sec.
  • The samples are centrifuged for 20 min. at 5000 rpm. 1 ml supernatant is mixed with 0,5 ml PHBAH reagent and boiled for 10 min. The test tubes are cooled in an ice water bath.
    • 3. Determination of Reducing End Groups:
  • The absorbency at 410 nm is measured using a spectrophotometer. Blanks are prepared by adding sodium hydroxide before adding enzyme solution.
  • A standard glucose curve was obtained by using glucose concentrations of 5, 10, 15 and 25 mg/l in the same buffer and adding PHBAH reagent before boiling. The release of reducing glucose equivalent is calculated using this standard curve.
    • 4. Calculation of Catalytic Activity:
  • Measure absorbance at 410 nm
    • 1) Standard Curve
  • (Glucose)-(H2O) vs concentration of glucose
    • 2) Enzyme Sample
  • (Sample)-(Blank)
    • Calculate Glucose Concentration According to a Standard Curve Activity (SAVIU/ml):
  • X ( mg glucose / l ) * Dilution 180.16 ( MW of glucose ) * 20 ( min )
  • C. Purification and Characterization of the Endoglucanase from M. Thermophila
  • Aspergillus oryzae transformed with pA2C193 was grown on YPM medium for 4 days. The liquid was then centrifuged and sterile filtered.
  • The sample was concentrated by ultrafiltration on AMICON cells using a DOW membrane GR61PP with cut-off 20 kD. The Uf-concentrate was analyzed for S-CEVU/ml and SaviU/ml with the following result:
  • UF-concentrate S-CEVU/ml SaviU/ml
    9.25 ml 570 41
    • Purification:
  • 2 ml of the UF-concentrate was diluted 5 times to tower the ionic strength and filtered through 0.22 micro-m disk fitter. This sample was applied to a Mono Q® HR5/5 Pharmacia column, equilibrated with 50 mM Tris/HCl buffer, pH 7.5, (buffer A) and a flow of 1 ml/min. After wash to baseline, with buffer A, the column was eluted with a Tris/HCl buffer, pH 7.5, containing 1 M NaCl (buffer B), the elution gradient was 0-50% buffer B in 1 hour.
  • After 36 min. a peak complex showed up, 1 ml fractions were picked up and the first 10 fractions showed cellulase activity on COM/Agarose/congo-red plates.
  • These fractions were pooled and concentrated, by ultrafiltration on AMICON cells using a DOW membrane GR61PP with cut-off 20 kD, to 3 ml.
  • This sample was applied to a HiLoad 26/60 Superdex 75™ prep grade Pharmacia column, equilibrated with 100 mM Na-Acetate buffer, pH 6.35, and a 1 ml/min flow.
  • After 82 min. a peak showed up, 1 ml fractions were picked up and the first 10 fractions showed cellulase activity on CMC/Agarose/congo-red plates.
  • These fractions were pooled and the following results were obtained;
    • A280=0.15 A280/A260=1.62 Mw(SDS)=22 kD
  • pl=3.5−5
    • Purity on SDS-PAGE=100% S-CEVU/ml=28.5 S-CEVU/A280=188 S-CEVU/mg-436
  • Extinction coefficient=54880 (calculated)
    • Mw(calculated)=22 kD
  • The Extinction coefficient is based on the content of tyrosine, tryptophane and cysteine calculated from the sequence of SEQ ID NO: 2 (the amino acid sequence). SOS-Page was performed on NOVEX Pre-Cast Gels 4-20% Tris-Glycine Gel 1.0 mm×10 Well.
  • IEF was performed on Pharmacia PAGplate pH 3.5-9.5, the activity was visualized by CMC-Congored overlaying.
  • Determination of KM & kcat:
  • km and kcat was determined in the same manner as the determination of SAVI Units at pH 8.5 with a substrate concentration up to 8 g/l.
  • The following results were obtained:
  • k cat 38 per sec.
    km 5 g/l,
    phosphoric acid swollen cellulose, pH 8.5.
    Specific activity on CMC at pH 7.5;
    436 S-CEVU per mg protein.
    D. Determination of pH and Temperature Profile of the Endoglucanase from M. thermophila
  • The pH profile was determined at the following conditions:
  • Buffers of pH values between 2.5 and 10.0 were made by mixing 0.1 M Tri-sodium phosphate with 0.1 M citric acid. Purified endoglucanase was diluted to ensure the assay response to be within the linear range of the assay. The substrate was a 0.4% suspension of AZCL-HE-cellulose (MegaZyme) mixed 1:1 with the citrate/phosphate buffer to a final substrate concentration of 0.2% AZCL-H E-cellulose. 1 ml substrate in Eppendorf® 1.5 ml polypropylene tubes were added 10 microliters of enzyme solution and incubated for 15 minutes in Eppendorf® temperature controlled Thermomixers before heat-inactivation of enzymes for 20 minutes at 95° C. in a separate Thermomixer. The tubes were centrifuged and 200 microliters of each supernatant was transferred to a well in a 96 well microtiter plate and OD was measured at 620 nm in an ELISA reader (Labsystems Multiskan® MCC/1340).
  • For the pH optimum incubations took place at 30° C. For each pH value, three tubes were added enzyme and incubated before heat-inactivation, whereas one tube (the blank) was added enzyme and heat-inactivated immediately. The mean value of the three incubated samples was calculated and the blank value was subtracted.
  • The following pH profile was determined:
  • pH Relative Activity
    2.5 <10% 
    3 <10% 
    3.5 22%
    4 87%
    4.5 89%
    5 100% 
    6 94%
    6.5 86%
    7 78%
    7.5 73%
    8 68%
    8.5 54%
    9 31%
    10 18%
  • It is seen that the endoglucanase has more than 60% activity between pH 4.0 and 8.0 and optima activity at pH 5.0-6.0.
    • Temperature Profile:
  • The temperature optimum was determined in the same manner at pH 5.5. The temperatures ranged from 30° C. to 80° C. For each temperature three incubations were carried out and the mean calculated. Three blanks were produced by immediate heat-inactivation of enzyme and the mean was subtracted from the incubated sample values.
  • Is seen that the endoglucanase has optimal activity at 50-70° C.
  • Temp. (° C.)
    30 40 50 60 70 80
    Relative Activity 74% 77% 99% 100% 93% 62%
  • The temperature stability was determined in the same manner at pH 5.5 and 30° C., and, further, the enzyme solutions were preheated for 1 hour at the actual temperature and cooled on ice. The residual activity is shown below in % of the activity of a non-preheated enzyme sample:
  • Temp. (° C.)
    40 50 60 70 80
    Relative Activity 95% 84% 92% 86% 24%

    E. Color Clarification of Myceliophthora Cellulase (SEQ ID NO: 2) Measured as Removal of Surface Fibrils and Fibers Protruding from the Yarn of a Textile Containing Cellulosic Fibers
  • Apparatus Terg-o-tometer
    Liquid volume 100 ml
    Agitation
    150 movements/min with vertical stirrer
    Rinse time 5 min in tapwater
    Washing temp 40° C.
    Washing liqour 0.05 M phosphate buffer
    pH 7.0
    Washing time 30 min
    Repetitions
    2
    Enzymes Myceliophthora cellulase (SEQ ID NO: 2)
    Dosage 500 and 2500 S-CEVU/l
    Textile
    2 swatches of aged black 100% cotton 5 × 6 cm
    (0.9 gram)
    Drying Tumble dry
    Evaluation The light remission is measured by a Datacolor
    Elrepho Remission spectrophotometer. Remission is
    calculated as delta L (Hunter Lab-values). When the
    surface fibrils and fibers protruding from the yarn
    are removed by the cellulase, the surface of the black
    fabric appears darker, and lower L values are obtained.
  • The sample is compared with a blind sample, i.e., washed without enzyme:
  • No cellulase 500 ECU/l 2500 ECU/l
    0.00 −1.41 −1.91
    • Delta L-Values Compared to Blind Sample.
  • The data shows that Myceliophthora cellulase without CBD gives good color clarification under the conditions tested.
  • F. Construction of the Gene Fusions Between the Endoglucanase from Myceliophthora thermophila and the 43 kD Endoglucanase from Humicola insolens
  • The purpose of the two constructions was to make derivatives of the endoglucanase from M. thermophila with the linker and CBD from the 43 kD endoglucanase from H. insolens (disclosed in WO 91/17243). The native endoglucanase from M. thermophila do not have a linker and/or a cellulose binding domain, CBD.
  • CM1: Construction 1 consists of the endoglucanase from M. thermophila (225 amino acids) and the 72 C-terminal amino acids from the H. insolens 43 kD endoglucanase.
  • CM2: Construction 2 consists of the endoglucanase from M. thermophila (225 amino acids) and the 83 C-terminal amino acids from the H. insolens 43 kD endoglucanase.
  • The 43 kD endoglucanase cDNA from H. insolens was cloned into pHD414 in such a way that the endoglucanase gene was transcribed from the Taka-promoter. The resulting plasmid was named pCaHj418.
  • In a similar way the cDNA encoding the endoglucanase from M. thermophila was cloned into in pHD414 and the resulting plasmid was named pA2C193. Primers;
  • primer 1:
    (SEQ ID NO: 88)
    5′-CGGAGCTCACGTCCAAGAGCGGCTGCTCCCGTCCCTCCAGCAGCACC
    AGCTCTCCGG-3′
    primer 2:
    (SEQ ID NO: 89)
    5′-CCGGAGAGCTGGTGCTGCTGGAGGGACGGGAGCAGCCGCTCTTGGAC
    GTGAGCTCCG-3′
    primer 3:
    (SEQ ID NO: 90)
    5′-CGGAGCTCACGTCCAAGAGCGGCTGCTCCCGTAACGACGACGGCAAC
    TTCCCTGCCG-3′
    primer 4
    (SEQ ID NO: 91)
    5′-CGGCAGGGAAGTTGCCGTCGTCGTTACGGGAGCAGCCGCTCTTGGAC
    GTGAGCTCCG-3′
    Take-pro primer:
    (SEQ ID NO: 92)
    5′-CAACATCACATCAAGCTCTCC-3′
    AMS-term primer:
    (SEQ ID NO: 93)
    5′-CCCCATCCTTTAACTATAGCG-3′
  • The endoglucanase fusions were constructed by the PCR overlap-extension method as described by Higuchi et al. 1988.
    • Construction 1:
  • Reaction A: The Polymerase Chain Reaction (PCR) was used to amplify the fragment of pCaHj418 between primer 1 and AMG-term. primer (the linker and CBD from the 43 kD endoglucanase from H. insolens).
    Reaction B. PCR amplification of the fragment between Taka-pro, primer and primer 2 in pA2C193, the endoglucanase gene from M. thermophila.
    Reaction C: The two purified fragments were used in a third PCR in the presence of the primers flanking the total region, i.e., Taka-pro. primer and AMG-term. primer.
    • Construction 2:
  • The same procedure was used where primer 3 and primer 4 had replaced respectively primer 1 and primer 2.
  • The fragment amplified in reaction C was purified, digested with restriction enzymes Xba I and BsstE II. The purified digested fragment was ligated into pA2C193 digested with restriction enzymes Xba I and BsstE II.
  • Competent cells from E. coli strain DH5αF′ (New England Biolabs.) were transformed with the ligated plasmid and colonies containing the gene fusion were isolated. The sequence of the cloned part was verified by DNA sequencing.
  • The sequences of the genes in the two constructs are SEQ ID NO: 3 and SEQ ID NO: 5.
  • Polymerase Chain Reactions were carried out under standard conditions, as recommended by Perkin-Elmer.
  • Reactions A and B started with 2 min. at 94° C. followed by 20 cycles of (30 sec. at 94° C., 30 sec. at 50° C. and 1 min. at 72° C.) and end with 4 min. at 72° C.
  • Reaction C started with (2 min. at 94° C., 1 min. at 52° C. and 2 min. at 72° C.), followed by 15 cycles of (30 sec. at 94° C., 30 sec. at 52° C. and 90 sec. at 72° C.) and end with 4 min. at 72° C.
  • The two constructs were transformed into Aspergillus oryzae as described above.
  • G. Purification and Characterization of Cloned Cellulases with Cellulose Binding Domains:
  • The cloned product is recovered after fermentation by separation of the extracellular fluid to from the production organism.
  • About one gram of cellulase is then highly purified by affinity chromatography using 150 gram of Avicel in a slurry with 20 mm Sodium-phosphate pH 7.5.
  • The Avicel is mixed with the crude fermentation broth, which contains total about 1 gram of cellulase. After mixing at 4° C. for 20 min the Avicel enzyme is packed into a column with a dimension of 50 times 200 mm about 400 ml total.
  • The column is washed with the 200 ml buffer, then washed with 0.5 M NaCl in the same buffer until no more protein elutes, Then washed with 500 ml 20 mm Tris pH 8.5. Finally the pure full length enzyme is eluted with 1% triethylamine pH 11.8.
  • The eluted enzyme solution is adjusted to pH 8 and concentrated using a Amicon cell unit with a membrane DOW GR61PP (polypropylene with a cut off of 20 KD) to above 5 mg protein per ml.
  • The purified cellulases were characterized as follows;
  •
    Mw S-CEVU
    SDS-PAGE pI Molar E.280 per A.280
    Myceliophthora 43 kD 4 74.950 135
    (SEQ ID NO: 4)
    Acremonium 40 kD 5 68.020 185
    (SEQ ID NO: 8)
    Thielavia 35 kD 4.3 52.470 75
    (SEQ ID NO: 12)
    pH Activity Thermostability
    above 50% N-terminal DSC
    Myceliophthora 5.0-9.0 Blocked. 80° C.
    (SEQ ID NO: 4)
    Acremonium 6.0-9.5 Blocked. 61° C.
    (SEQ ID NO: 8)
    Thielavia 5.0-9.0 ASGSG--- 83° C.
    (SEQ ID NO: 12)
  • The purified cellulases was analysed for MW by SDS-PAGE and using standard LMW protein marker kit from Pharmacia the MW was calculated for the cellulases. The MW is apparently higher than the MW of the composition of the coding amino acids and is due to the fact the linker region is O-glycosylated resulting in this higher MW. The pt was determined using a Pharmacia Ampholine PAG plate pH 3.5 to 9.5 and again using a Pharmacia kit with known pl proteins.
  • The molar extinction coefficient was calculated based on the amin acids composition using the known absorbance of Tryptophan, Tyrosine and Cystein.
  • pH activity profile was obtained using CMC substrate, incubation for 20 min at 40° C. at a 0.5 pH interval and measuring the formation of reducing sugars. The relative activity at the different pH was calculated and the table contains the intervat with more than 50% relative activity has been measured.
  • The N-terminal was determined for the purified cellulase using a Applied Biosystems model 473A sequencer. The protein sequencer was run according to the manufacturer's instructions.
  • Two of the cellulases were blocked, this is due to the N-terminal glutamine which forms a MC pyroglutamate which can not be detected and which blocks further sequencing.
  • Differential scanning calometry (“DSC”) was done at neutral pH (7.0) using a MicroCalc Inc. MC calorimeter with a constant scan rate and raising the temperature from 20 to 90° C. at a rate of 900 per hour.
  • Raising antibody. The cellulases from Myceliophthora, Acremonium and Thielavia were used for raising antibody in rabbits. 0.1 mg of the purified cellulases in 0.9% NaCl solution mixed with Freunds adjuvant immediately prior to injection. The rabbits were immunized 10 times with one week interval. The immunoglobulin G fraction (IgG) was purified by ammonium sulfate precipitation (25% saturation). The precipitate was solubilized in water and then dialyzed extensively against sodium acetate buffer (pH 5.0, 50 mM) altering with deionized water. After filtration, the IgG fraction was stabilized with sodium azide (0.01%).
  • Using immunodiffusion in agar plates all three cellulases form a single immunoprecipitate with its homologous antiserum and no precipitate was seen between the 3 cloned cellulases and the sera raised against the other two cellulases.
  • H-I. Performance of Endoglucanase of Construction 1 (SEQ ID NO: 3) Measured in Buffer as Removal of Surface Fibrils and Fibers Protruding from the Yarn of a Textile Containing Cellulosic Fibers
  •
    Apparatus Terg-o-tometer
    Liquid volume 100 ml
    Agitation
    150 movements/min (rpm)
    Rinse time 5 min in tap water
    Washing temp 40° C.
    Water Hardness
    1 mM CaCl2
    Washing liquor 0.05 M phosphate buffer
    pH 7.0
    Washing time 30 min
    Repetitions
    2
    Textile 2 swatches of aged black, 100% cotton 5 × 6 cm
    Drying Tumble dry
    • Evaluation:
  • The light remission was measured by a Macbeth Color Eye 7000 Remission spectrophotometer. Remission is calculated as delta L (Hunter Lab-values). When the surface fibrils and fibers protruding from the yarn were removed by the cellulase, the surface appeared more bright and lower L values were obtained.
    • Results:
  • S-CEVU/l 0 250 1000
    Inventive enzyme 0 −1.4 −1.6
  • The data show that the enzyme of the invention gives very good color clarification under the conditions tested.
  • H-I. Performance of Cloned Endoglucanase from Thielavia terrestris (SEQ ID NO: 12) in Buffer Measured as Removal of Surface Fibrils and Fibers Protruding from the Yarn of a Textile Containing Cellulosic Fibers
  •
    7Apparatus Terg-o-tometer
    Liquid volume 100 ml
    Agitation
    150 movements/min with vertical stirrer
    Rinse time 10 min in tapwater
    Washing temp 40°
    Washing liquor 0.05 M phosphate buffer.
    pH 7.0
    Washing time 30 min
    Repetitions
    2
    Textile 2 swatches of aged black cotton 5 × 6 cm
    (app. 150 g/m2)
    Drying Tumble dry
    • Evaluation:
  • The light remission was measured by a Datacolor Elrepho Remission spectrophotometer. Remission is calculated as delta L (Hunter Lab-values). When the surface fibrils and fibers protruding from the yarn are removed by the cellulase, the surface of the black fabric appears darker and nicer, and lower L values are obtained.
    • Results:
  • S-CEVU/l 0 50 200
    Inventive enzyme 0 −0.66 ± 0.10 −1.32 ± 0.06
  • The data show that the cellulose gives good color clarification under the conditions tested.
  • H-III Performance Of Endoglucanase Of Volutella colletrichoides (SEQ ID NO: 9) Measured in Buffer as Removal of Surface Fibrils and Fibers Protruding from the Yarn of a Textile Containing Cellulosic Fibers
  •
    Apparatus Terg-o-tometer
    Liquid volume 100 ml
    Agitation
    150 movements/min with vertical stirrer
    Rinse time 5 min in tapwater
    Washing temp 40°
    Washing liqour 0.05 M phosphate buffer
    pH 7.0
    Washing time 30 min
    Repetitions
    2
    Dosage 2.5 S-CEVU/ml
    Textile
    2 swatches of aged black 100% cotton 5 × 6 cm
    (0.9 gram)
    Drying Tumble dry
    • Evaluation,
  • The light remission is measured by a Datacolor Elrepho Remission spectrophotometer. Remission is calculated as delta L (Hunter Lab-values). When the surface fibrils and fibers protruding from the yarn are removed by the cellulase, the surface of the black fabric appears darker, and lower L values are obtained.
  • The sample is compared with a blind sample, i.e., washed without enzyme:
  • No cellulase With cellulase
    0.00 −0.57

    Delta L remission values compared to blind sample.
  • The data shows that the Volutella colletrichoides cellulase gives good color clarification under the conditions tested.
  • H-IV. Performance of Cloned Cellulases from Thielavia terrestris and Acremonium sp. CBS 478.94 in high pH Heavy Duty Detergent Measured as Removal of Surface Fibrils and Fibers Protruding from the Yarn of a Textile Containing Cellulosic Fibers
  •
    Apparatus Terg-o-tometer
    Liquid volume
    150 ml
    Agitation
    150 movements/min with vertical stirrer
    Rinse time 10 min in tapwater
    Washing temp 35° C.
    Washing liqour 1.0 g/l US type HDG
    (zeolite/soda built, anionic/nonionic weight ratio >2.5)
    pH 10.0
    Hardness 1.0 mM CaCl2
    0.34 mM MgCl2
    Washing time 12 min
    Repetitions 6
    Textile 2 swatches of aged black cotton 5 × 6 cm
    (app. 150 g/m2)
    2 swatches of heavy knitted cotton 5 × 6 cm
    (app. 600 g/m2)
    Drying Tumble dry
    • Evaluation:
  • The light remission is measured by a Datacolor Elrepho Remission spectrophotometer. Remission is calculated as delta L (Hunter Lab-values). When the surface fibrils and fibers protruding from the yarn are removed by the cellulase, the surface of the black fabric appears darker and nicer, and lower L values are obtained. Different dosages of cloned cellulases from Thielavia terrestris (SEQ ID NO: 12) and Acremonium sp. CBS 478.94 (SEQ ID NO: 8), respectively, (denoted A and B, respectively) were tested.
    • Results:
  • S-CEVU/l 0 500 2000
    A 0 −2.09 ± 0.22 −2.86 ± 0.19
    B 0 −0.60 ± 0.36 −1.96 ± 0.23
  • The data show that both cellulases gives good color clarification under the conditions tested.
  • H-V. Performance of Cellulases Cloned from Thielavia terrestris and Acremonium sp. CBS 478.94, and Construction 1 (SEQ ID NO: 3) Measured as Removal of Surface Fibrils and Fibers Protruding from the Yarn of a Textile Containing Cellulosic Fibers
  •
    Apparatus Terg-o-tometer
    Liquid volume
    150 ml
    Agitation
    150 movements/min with vertical stirrer
    Rinse time 10 min in tapwater
    Washing temp 35° C.
    Hardness 1.0 mM CaCl2
    0.34 mM MgCl2
    Washing liqour 2.0 g/l HDL (neutral, citrate built HDL,
    with nonionic/anionic weight ration >0.5)
    pH 7.5
    Washing time 30 min
    Repetitions
    2
    Textile 2 swatches of aged black cotton 5 × 6 cm
    (app. 150 g/m2)
    2 swatches of heavy knitted cotton 4 × 7 cm
    (app. 600 g/m2)
    Drying Tumble dry
    • Evaluation:
  • The light remission is measured by a Datacolor Elrepho Remission spectrophotometer. Remission is calculated as delta L (CIE Lab-values). When the surface fibrils and fibers protruding from the yarn are removed by the cellulase, the surface of the black fabric appears darker and nicer, and lower L values are obtained. Three different dosages of cloned cellulases from Thielavia terrestris (SEQ ID NO: 12) and Acremonium sp. CBS 478.94 (SEQ ID NO: 8) and the construction 1 (SEQ ID NO: 3), respectively, (denoted A and B and C, respectively) were tested.
    • Results:
  • S-CEVU/l 0 100 200 400
    A 0 −3.06 ± 0.24 −3.15 ± 0.27 −3.92 ± 0.26
    B 0 −1.75 ± 0.27 −3.08 ± 0.32 −3.51 ± 0.44
    C 0 −1.84 ± 0.39 −1.70 ± 0.47 −2.30 ± 0.61
  • The data show that all cellulases give very good color clarification under the conditions tested.
  • I. Application of Endoglucanases from Thielavia terrestris, Acremonium sp. and Construction 1 (SEQ ID NO: 3) in Denim Finishing
    • Experimental
  • Apparatus: Washing machine Wascator FL 120
    Liquid volume: 20 L
    Fabric: 1.1 kg denim fabric, 14½ oz 100% cotton
    Desizing: 10 min, 55° C., pH 7
    50 ml Aquazyme 120 L
    2.5 g/l Phosphate buffer
    Abrasion: 2 hours;
  • pH and temperature varied according to the following table:
  • Enzyme
    SEQ ID Activity pH/temp Buffer system
    NO: 3 1400 S-CEVU/g 6/55° C. 2.5 g/l phosphate buffer
    NO: 12  292 S-CEVU/g 5/65° C.   1 g/l citrate buffer
    NO: 8  782 S-CEVU/g 7/45° C. 2.5 g/l phosphate buffer

    Inactivation: 15 min, 80° C.
  • 1 g/l sodium carbonate
  • Rinses: Three rinse cycles of 5 min in cold tap water
    • Evaluation:
  • Abrasion: The remission from the fabric was determined at 420 nm using a Texflash 2000 as a measure of the abrasion level.
  • The results from the treatment of the denim fabric with different endoglucanases of the invention is shown in the following table:
  • Abrasion
    Enzyme Dosage Trial conditions 420 nm
    Blank  0 S-CEVU/g textile pH 6, 55° C. 9.96
    SEQ ID NO: 3 10 S-CEVU/g textile pH 6, 55° C. 14.37
    Blank  0 S-CEVU/g textile pH 5, 65° C. 9.26
    SEQ ID NO: 12 10 S-CEVU/g textile pH 5, 65° C. 16.86
    Blank  0 S-CEVU/g textile pH 7, 45° C. 9.47
    SEQ ID NO: 8 10 S-CEVU/g textile pH 7, 45° C. 14.08
  • All tested cellulases show excellent performance in denim finishing, although each enzyme is unique in its own way, When applying the enzyme corresponding to SEQ ID NO: 3 for denim finishing it is possible to reach a high abrasion level with a minimum of strength loss. When treating denim with the enzyme corresponding to SEQ ID NO: 12, a very high wash down can be reached which leaves the fabric with an almost bleached appearance. Denim finishing with the enzyme corresponding to SEQ ID NO: 8 gives a high abrasion level at a tow temperature optimum which makes it possible to reduce the processing temperature and save energy.
  • J. Use of Cloned Cellulases from Acremonium sp. and Thielavia Terrestris for Biopolishing of Lyocell Fibers
  • Lyocell fibers which are sold under the trade name Tencel are spun from wood pulp its cellulose in a more environmentally friendly waterbased solvent than is the case for normal viscose production). How ever, the fibers have a tendency to fibrillate when they are processed into textiles which is seen on the surface and denoted “fuzz”. By using cellulases it is possible to permanently remove the exposed and Fuzzy fibers and significantly improve the look of the finished fabric, the treatment generally known as Biopolishing. The endoglucanases of the present invention are especially suited for the removal of Lyocell surface fibers,
    • Materials and Methods
  • The textile substrate was either 100% woven or different kinds of jersey knitted dark blue almost. The dark colour and jersey knit was preferred in order to enhance the visual effects which simplified the evaluation. A woven 70/30 Tencel/Rayon blend was also used to a lesser extent.
  • The assays were either performed in 200 ml scale using a Launder-o-meter or in the 20 l scale using a Wascator. The treatment time was 60 min at 55° C. in Wascator and 60-90 min in LOM. The buffer was 2 g/l sodium acetate adjusted to pH 5 with acetic acid, The fabric to liquid ratio was 1:10 but in the Launder-o-meter 20 steel balls with a diameter of 14 mm (11 g each) was used to obtain sufficient mechanical abrasion. The biopolishing was immediately followed by inactivation using 2 g/l sodium carbonate at 80° C. for 15 min followed by rinsing in cold water.
  • The results were evaluated using a fuzz note scale from 1-5 were 1 is the fibrillated look of the starting material and 5 is a high quality look with no visible fibers on the surface. Since the performance of endocellulases is specific towards a surface treatment the weightloss is below 2% and is therefore not included in the evaluation. Two cellulases were evaluated: the cellulases cloned from Acremonium sp. (SEQ ID NO: 8) and from Thielavia terrestris (SEQ ID NO: 12).
  • The two cellulases are able to defibrillate both Tencel and Tencel blended fabrics. By using an endoglucanase of the invention, only small fibrils are removed rather than whole fibers such as is the case when using acid cellulase mixtures from Trichoderma. The strength loss of the treated fabric is therefore kept at a minimum when using endoglucanases of the present invention.
  • The following dosages gave a superior defibrillation, i.e., fuzz note 4 or above:
  • 15 S-CEVU/g fabric of cellulase from Acremonium sp (SEQ ID NO: 8); and
    10 S-CEVU/g fabric of cellulase from Thielavia terrestris (SEQ ID NO: 12).
    • EXAMPLE 2 A New Cellulytic Enzyme was by Expression Cloning as Well as by PCR Cloning Detected to be Produced by a Plant Pathogen, Isolated from Soy Bean Seeds and Identified as Macrophomina Phaseolina Production of Biomass for PCR and Expression Cloning Procedures:
  • Isolate CBS 281.96 was grown in shake flask cultures on cellulose enriched potato dextrose broth, incubated for 5 days at 260 C (shaking conditions: 150 rpm).
  • A. Cloning and Expression of an Endoglucanase from Macrophomina Phaseolina
  • mRNA was isolated from Macrophomina phaseolina, grown in a cellulose-containing fermentation medium with agitation to ensure sufficient aeration. Mycelia were harvested after 3-5 days' growth, immediately frozen in liquid nitrogen and stored at −80° C. A library from Macrophomina phaseolina, consisting of approx. 106 individual clones was constructed in E. coli as described with a vector background of 1%.
  • Plasmid DNA from some of the pools was transformed into yeast, and 50-100 plates containing 250-400 yeast colonies were obtained from each pool.
  • Endoglucanase-positive colonies were identified and isolated on SC-agar plates with the AZCL HE cellulose assay, cDNA inserts were amplified directly from the yeast colonies and characterized as described in the Materials and Methods section above. The DNA sequence of the cDNA encoding the endoglucanase is SEQ ID NO: 13 and the corresponding amino acid sequence is SEQ ID NO: 14.
  • The cDNA is obtainable from the plasmid in DSM 10512.
  • Total DNA was isolated from a yeast colony and plasmid DNA was rescued by transformation of E. coli as described above. In order to express the endoglucanase in Aspergillus, the DNA was digested with appropriate restriction enzymes, size fractionated on gel, and a fragment corresponding to the endoglucanase gene was purified, The gene was subsequently ligated to pHD414, digested with appropriate restriction enzymes, resulting in the plasmid pA2C477.
  • After amplification of the DNA in E. coli the plasmid was transformed into Aspergillus oryzae as described above.
  • Screening of the cDNA Library by Hybridization and Characterization of the Positive Clones.
  • Approximately 6000 colony forming units (c.f.u.) from the Macrophomina phaseolina cDNA library in E. coli was screened by colony hybridization using a random-primed 32P-labeled PCR to product from M. phaseolina as probe, The PCR product was generated as described in the Materials and Methods section. The positive cDNA clones were characterized by sequencing the ends of the cDNA inserts, and by determining the nucleotide sequence of the longest cDNA from both strands. The DNA sequence of the cDNA encoding the endoglucanase is SEQ ID NO: 13 and the corresponding amino acid sequence is SEQ ID NO: 14.
  • B. Construction of Gene Fusion Between the Endoglucanase from Macrophomina Phaseolina and the 43 kD Endoglucanase from Humicola insolens
  • One construction was prepared in order to make a derivative of the endoglucanase from M. phaseolina with the linker and CBD from the 43 kD endoglucanase from H. insolens (disclosed in WO 91/17243) The native endoglucanase from M. phaseolina does not have a linker and/or a cellulose binding domain, CBD.
  • The construction consists of the endoglucanase from M. phaseolina (222 amino acids) and the 72 C-terminal amino acids from the H. insolens 43 kD endoglucanase (SEQ ID NO: 24).
  • The 43 kD endoglucanase cDNA from H. insolens is cloned into pHD414 in such a way that the endoglucanase gene is transcribed from the Taka-promoter. The resulting plasmid is named pCaHj418.
  • The cDNA encoding the endoglucanase from M. phaseolina (SEQ ID NO: 13) is cloned into pYES2.0 as a BstX I/Not I fragment and the resulting plasmid is named pC1C477.
  • Primers:
  • primer 1:
    (SEQ ID NO: 94)
    5′-GGTCGCCCGGACTGGCTGTTCCCGTACCCCCTCCAGCAGCACCAGCT
    CTCCGG-3′
    primer 2:
    (SEQ ID NO: 95)
    5′-CCGGAGAGCTGGTGCTGCTGGAGGGGGTACGGGAACAGCCAGTCCGG
    GCGACC-3′
    pYES2.0 F.HT primer:
    (SEQ ID NO: 96)
    5′-CGGACTACTAGCAGCTGTAATACG-3′
    AMO-term Primer:
    (SEQ ID NO: 93)
    5′-CCCCATCCTTTAACTATAGCG-3′
  • The endoglucanase fusion is constructed by the PCR overlap-extension method as described by Higuchi et al. 1988.
  • Reaction A: The Polymerase Chain Reaction (PCR) is used to amplify the fragment of pCaHj418 between primer 1 and AMG-term. primer (the linker and CBD from the 43 kD 2 endoglucanase from H. insolens).
  • Reaction B: PCR amplification of the fragment between pYES2.0 F.HT primer and primer 2 in pC1C477, the endoglucanase gene from M. phaseolina.
  • Reaction C. The two purified fragments are used in a third PCR in the presence of the primers flanking the total region, i.e., pYES2.0 F.HT primer and AMG-term. primer.
  • The fragment amplified in reaction C is purified, digested with restriction enzymes, e.g. Xba I and BamHI. The purified digested fragment is ligated into pHD414 digested with restriction enzymes, e.g., Xba I and BamH I.
  • Competent cells from E. coli strain DH5αF′ (New England Biolabs) are transformed with the ligated plasmid and colonies containing the gene fusion are isolated. The sequence of the cloned to part was verified by DNA sequencing.
  • Polymerase Chain Reactions are carried out under standard conditions, as recommended by Perkin-Elmer.
  • Reaction A and B start with 2 min. at 94° C. followed by 20 cycles of (30 sec. at 94° C., 30 sec. at 52° C. and 1 min. at 72° C.) and ends with 4 min. at 72° C.
  • Reaction C starts with (2 min. at 94° C., 1 min. at 52° C. and 2 ml. at 72° C.), followed by 20 cycles of (30 sec. at 94° C., 30 sec. at 52° C. and 90 sec. at 72° C.) and ends with 4 min. at 72° C.
  • The construct may be transformed into Aspergillus oryzae as described above.
    • EXAMPLE 3 Cloning and Expression of an Endoglucanase from Acremonium sp. and Sordaria fimicola Production of Biomass for Expression Cloning Procedures
  • Isolates CBS 478.94 and ATCC 52644, respectively, were grown in shake flask cultures on cellulose enriched potato dextrose broth, incubated for 5 days at 260 C (shaking conditions: 150 rpm).
  • mRNA was isolated from Acremonium sp., CBS 478.94, and Sordaria fimicola, ATCC 52644, respectively, grown in a cellulose-containing fermentation medium with agitation to ensure sufficient aeration. Mycelia were harvested after 3-5 days' growth, immediately frozen in liquid nitrogen and stored at −80° C. Libraries from Acremonium sp., and Sordaria fimicola, respectively, each consisting of approx. 106 individual clones were constructed in E. coli as described with a vector background of 1%.
  • Plasmid DNA from some of the pools from each library was transformed into yeast, and 50-100 plates containing 250-400 yeast colonies were obtained from each pool.
  • Endoglucanase-positive colonies were identified and isolated on SC-agar plates with the AZCL HE cellulose assay. cDNA inserts were amplified directly from the yeast colonies and characterized as described in the Materials and Methods section above.
  • The DNA sequence of the cDNA encoding the endoglucanase from Acremonium sp. is SEQ ID NO: 9 and the corresponding amino acid sequence is SEQ ID NO: 10. The cDNA is obtainable from the plasmid in DSM 10080.
  • The partial DNA sequence of the cDNA encoding the endoglucanase from Sordaria fimicola is SEQ ID NO-25 (Nucleotide sequence of the 5′-end of the cDNA) and the corresponding amino acid sequence is SEQ ID NO: 26. The cDNA is obtainable from the plasmid in DSM 10576.
  • Total DNA was isolated from a yeast colony and plasmid DNA was rescued by transformation of E. coli as described above. In order to express the endoglucanase in Aspergillus, the DNA was digested with appropriate restriction enzymes, size fractionated on gel, and a fragment corresponding to the endoglucanase gene from Acremonium sp. and Sordaria fimicola, respectively, was purified. The genes were subsequently ligated to pHD414, digested with appropriate restriction enzymes, resulting in the plasmids pA2C371 and pA2C502, respectively.
  • After amplification of the DNA in E. coli the plasmids were transformed into Aspergillus oryzae as described above.
    • EXAMPLE 4 A. Cloning by PCR an Endoglucanase from Crinipellis scabella, CBS 280.96
  • Isolate CBS 280.96 was grown in static flask cultures, holding wheat bran medium (per flask, 300 g wheat bran added 450 ml salt solution), incubated for 6 days at 26° C. After incubation the wheat bran was extracted with distilled water (300 ml per flask) and the extract tested for endoglucanase activity (0.1% AZCL-HE-Cellulose (megazyme) in 1% agarose (Litex agarose, Medinova). Activity was observed on the plates holding pH of 3.0, 7.0 and 9.5.
  • mRNA was isolated from Crinipellis scabella grown as describe above. Mycelia were harvested after 3-5 days' growth, immediately frozen in liquid nitrogen and stored at −80° C. A library from Crinipellis scabella, consisting of approx. 106 individual clones was constructed in E. coli as described with a vector background of 1%.
  • Approximately 10 000 colony forming units (c.f.u.) from the Crinipellis scabella cDNA library in E. coli was screened by colony hybridization using a random-primed 32P-labeled PCR product from C. scabella as probe. The PCR product was generated as described in the Materials and methods section. The positive cDNA clones were characterized by sequencing the ends of the cDNA inserts, and by determining the nucleotide sequence of the longest cDNA from both strands.
  • The DNA sequence of the cDNA encoding the endoglucanase is SEQ ID NO: 15 and the corresponding amino acid sequence is SEQ ID NO: 16.
  • The cDNA is obtainable from the plasmid in DSM 10511.
  • Total DNA was isolated from a yeast colony and plasmid DNA was rescued by transformation of E. coli as described above. In order to express the endoglucanase in Aspergillus, the DNA was digested with appropriate restriction enzymes, size fractionated on gel, and a fragment corresponding to the endoglucanase gene was purified. The gene was subsequently ligated to pHD414, digested with appropriate restriction enzymes, resulting in the plasmid pA2C475.
  • After amplification of the DNA in E. coli the plasmid was transformed into Aspergillus oryzae as described above.
  • Construction of Two Gene Fusions Between the Endoglucanase from Crinipellis Scabella and the Linker/CBD Region of the 43 kDa Endoglucanase from Humicola insolens.
  • The native endoglucanase from Crinipellis scabella neither has a linker nor a cellulose binding domain (CBD). In addition, the full-length cDNA contains an ATG start codon upstream from the endoglucanase encoding open reading frame (ORF), presumably resulting in scrambled translation initiation upon heterologous expression of the cDNA, such as in the yeast Saccharomyces cerevisiae and the filamentous fungus Aspergillus oryzae. Thus, two gene fusions between the endoglucanase from Crinipellis scabella and the linker/CBD region of the 43 kD endoglucanase from Humicola insolens (disclosed in WO 91/17243) has been constructed using splicing by overlap extension (SOE) (Horton et al, 1989).
  • Construction 1 consists of the cDNA encoding the 226-residue endoglucanase from C. scabella fused by PCR with the 3′-end cDNA of H. insolens coding for the linker and CBD region (72 amino acids) at the COOH-terminus of the H. insolens 43 kD endoglucanase. The second hybrid construct is identical to the abovementioned gene fusion, except that the first five residues from the putative signal peptide have been deleted by PCR resulting in a shorter signal, which starts with the second in-frame ATG start codon.
    • Plasmid Constructs
  • The plasmid pC1C475 contains the full-length cDNA from C. scabella, cloned into BstXI/NotI-cut yeast expression vector pYES 2.0, the plasmid pC1C144 contains the full-length cDNA from H. insolens, cloned into the BstXI site of pYES 2.0.
    • Splicing by Overlap Extension
  • Two PCR fragments encoding the core region of the endoglucanase from C. scabella were generated in PCR buffer (10 mM Tris-HCl, pH 8.3, 50 mM KCl, 1.5 mM MgCl2, 0.01% gelatin; containing 200 micro-M each dNTP), using 50-100 ng of pC1C475 as template, and 250 pmol of the reverse primer (5′-GACCGGAGAGCTGGTGTGCTGGAGGGTTTACGACACAGCCCGAGATATAGTG-3 (SEQ ID NO: 97)) in two combinations with 300-350 pmol of each forward primer (forward no. 1 5′-CCCCAAGTTGACTTGGAACCAATGGTCCATCG-3′ (SEQ ID NO: 98), forward no. 2 5′-CCCCAAGCTTOCATOCAAACATGCTTAAAACOGTOG-3, (SEQ ID NO: 99)), a DNA thermal cycler (Landgraf, Germany) and 2.5 units of Taq polymerase (Perkin-Elmer, Cetus, USA). Thirty cycles of PCR were performed using a cycle profile of denaturation at 94° C. for 1 min, annealing at 55° C. for 2 min, and extension at 72° C. for 3 min. The PCR fragment coding for the linker and CBD of the endoglucanase of H. insolens was generated in PCR buffer (10 mM Tris-HCl, pH 8.3, 50 mM KCl, 1.5 mM MgCl2, 0.01% gelatin: containing 200 micro-M each dNTP) using 100 ng of the pC1C144 template, 250 pmol forward primer (5′-CACTAATATCTCGGCTGTGTTCGTAACCCTCCAGCAGCACCAGCTCTCCGGTC-3′(SEQ ID NO:100)), 250 pmol of the pYES 2.0 reverse primer (5′-GGGCGTGAATGTAAGCGTGACATA-3′ (SEQ ID NO-101)), a DNA thermal cycler (Landgraf, Germany) and 2.5 units of Taq polymerase (Perkin-Elmer, USA). Thirty cycles of PCR were performed as above. The PCR products were electrophoresed in 0.7% low gelling temperature agarose gels (SeaPlaque, FMC), the fragments of interest were excised from the gel and recovered by treatment with agarase (New England Biolabs, USA) according to the manufacturer's instructions, followed by phenol extraction and ethanol precipitation at −20° C. for 12 h by adding 2 vols of 96% EtOH and 0.1 vol of 3 M NaAc.
  • The recombinant hybrid genes between the endoglucanase from Crinipellis scabella and the linker/CBD region of the 43 kD endoglucanase from Humicola insolens were generated by combining the overlapping PCR fragments from above (ca 50 ng of each template) in two combinations in PCR buffer (10 mM Tris-HCl, pH 8.3, 50 mM KCl, 1.5 mM MgCl2, 0.01% gelatin; containing 200 micro-M each dNTP). The SOE reaction was carried out using the DNA thermal cycler (Landgraf, Germany) and 2.5 units of Taq polymerase (Perkin-Elmer, Cetus, USA). Two cycles of PCR were performed using a cycle profile of denaturation at 94° C. for 1 min, annealing at 55° C. for 2 min, and extension at 72° C. for 3 min, the reaction was stopped, 250 pmol of each end-primer (forward no. 1 5′-CCCCAAGCTTGACTTGGAACCAATGGTCCATCC-3′ (SEQ ID NO: 98), forward no. 2 5′-CCCCAAGCCTCCATCCAAACATGCTTAAAACGCTCG-3′ (SEQ ID NO: 99), reverse primer 5′GGGCGCTGAATGTAAGCGTGACATA-3′ (SEQ ID NO: 101)) was added to the reaction mixture, and an additional 30 cycles of PCR were performed using a cycle profile of denaturation at 94° C. for 1 min annealing at 55° C. for 2 min, and extension at 72° C. for 3 min.
  • Construction of the Expression Cassettes for Heterologous Expression in Aspergillus oryzae
  • The PCR-generated, recombinant fragments were electrophoresed in a 0.7% low gelling temperature agarose gel (SeaPlaque, FMC), the fragments of interest were excised from the gel and recovered by treatment with agarase (New England Biolabs, USA) according to the manufacturer's instructions, followed by phenol extraction and ethanol precipitation at −20° C. for 12 h. The DNA fragments were digested to completion with HindIII and XbaI, and ligated into HindIII/XbaI-cleaved pHD414 vector followed by electroporation of the constructs into E. coli DH10B cells according to the manufacturer's instructions (Life Technologies, USA).
  • The nucleotide sequence of the resulting gene fusions was determined from both strands as described in the Materials and methods section, SEQ ID NOS: 17 and 19. The constructs may be transformed into A. oryzae as described.
    • EXAMPLE 5 PCR Facilitated Detection of Said Type of Cellulytic Enzyme from 46 Filamentous and Monocentric Fungi, Representing 32 Genera, from 23 Families, Belonging to 15 Orders of 7 Classes, Covering all in all all Four Groups of the True Fungi: Ascomycetous, Basidiomnycetous, Chytridiomycetous and Zygomycetous fungi 5.1 Materials
  • 1. Diplodia gossypina Cooke
    • Deposit of Strain, Acc No: CBS 274.96
  • 2. Ulospora bilgramii (Hawksw et al.)
    Acc No of strain: NKBC 1444,
    • 3. Microsphaeropsis sp
  • 4. Ascobolus stictoideus Speg.
    Acc No of strain: Q026 (Novo Nordisk collection)
    Isolated from goose dung, Svalbard, Norway
    5. Saccobolus dilutellus (Fuck) Sacc.
    Deposit of strain: Acc No CBS 275.96
    6. Penicillium verruculosum Peyronel
    Ex on Acc No of species ATCC 62396
    7. Penicillium chrysogenum Thom
    • Acc No of Strain: ATCC 9480
  • 8. Thermomyces verrucosus Pugh et al
    • Deposit of Strain, Acc No.: CBS 285.96
  • 9. Xylaria hypoxylon L. ex Greville
    Deposit of Strain. Acc No: CBS 284.96
    10. Poronia punctata (Fr. ex L.) Fr.
    • Ref:A.Munk: Danish Pyrenomycetes, Dansk Botanisk Arkiv, Vol 17, 1 1957 11. Nodulisporam sp
  • Isolated from leaf of Camellia reticulatá (Theaceae, Guttiferales),
    • Kunming Botanical Garden, Yunnan Province, China 12. Cylindrocarpon sp
  • Isolated from marine sample, the Bahamas
    13. Fusarium anguioides Sherbakoff
    Acc No of strain: IFO 4467
    14. Fusarium poae (Peck) WV.
    Ex on Acc No of species: ATCC 60883
    15. Fusarium solani (Mart.)Sacc.emnd.Snyd & Hans.
    Acc No of strain: IMI 107.511
    16. Fusarium oxysporum ssp lycopersici (Sacc.)Snyd. & Hans.
    Acc No of strain: CBS 645.78
    17. Fusarium oxysporum ssp pass/flora
    Acc No of strain: CBS 744.79
    18. Gliocladium catenulatum Gilman & Abbott
    Acc. No of strain: ATCC 10523
    19. Nectria pinea Dingley
    Deposit of Strain, Acc. No. CBS 279.96
    20. Sordań a macrospora Auerswald
    ex on Acc No of species. ATCC 60255
    21. Humicola grisea Traeen
    ex on Acc No for the species: ATCC 22726
    22. Humicola nigrescens Omvik
    Acc No of strain: CBS 819.73
    23. Scytalidium thermophilum (Cooney et Emerson) Austwick
    Acc No of strain: ATCC 28085
    24. Thielavia thermophila Fergus et Sinden
    (syn Corynascus thermophilus)
    Acc No of strain: CBS 174.70, IMI 145.136
    25. Cladorrhinum foecundissimum Saccardo et Marchal
    Ex on Acc No of species: ATCC 62373
    26. Syspastospora boninensis
    Acc No of strain: NKBC 1515 (Nippon University, profe Tubaki Collection)
    27. Chaetomium cuniculorum Fuckel
    Acc. No, of strain: CBS 799.83
    28. Chaetomium brasiliense Batista et Potual
    Acc No of strain: CBS 122.65
    29. Chaetomium murorum Corda
    Acc No of strain: CBS 163.52
    30. Chaetomium virescens (von Arx) Udagawa
    Acc. No. of strain: CBS 547.75
    • 31. Nigrospora sp
  • Deposit of strain, Acc No: CBS 272.96
    • 32. Nigrospora sp
  • Isolated from:
    33. Diaporthe syngenesia
    Deposit of strain, Acc No: CBS 278.96
    34. Colletotrichum lagenarium (Passerini) Ells et Halsted
    syn Glomerella cingulata var orbiculare Jenkins et Winstead
    Ex on acc No of species: ATCC 52609
    35. Exidia glandulosa Fr.
    • Deposit of Strain, Acc No: CBS 277.96
  • 36. Fomes fomentarius (L.) Fr.
    deposit of strain: Ac No. CBS 276.96
    • 37. Spongipellis (?) Deposit of Strain, Acc No CBS 283.96
  • 38. Rhizophlyctis rosea (de Bary & Wor) Fischer
    • Deposit of Strain: Acc No.: CBS 282.96
  • 39. Rhizomucor pusillus (Lindt) Schipper
    syn: Mucor pusillus
    Acc No of strain: IFO 4578
    40. Phycomyces nitens (Kunze) van Tieghem & Le Monnier
    Acc No of strain: IFO 4814
    41. Chaetostylum fresenii van Tieghem & Le Monnier
    syn. Helicostylum fresenii
    Acc No of strain NRRL 2305
    42. Trichothecium roseum, Acc No of strain: IFO 5372
    • 43. Coniothecium sp,
  • Endophyte, isolated from leaf of flowering plant,
    • Kunming, Yunnan, China
  • 44. Deposit of strain, Acc No., CBS 271.96
    Coelomycete, isolated from leaf of Artocarpus altilis
    • (Moraceae, Urticales), Christiana, Jamaica
  • 45, Deposit of strain, Acc No.: CBS 273.96
    Coelomycete, isolated from leaf of Pimenta dioica
    • (Myrtaceae, Myrtales), Dallas Mountain, Jamaica
  • 46. Deposit of strain, CBS 270.96
    Coelomycete, isolated from leaf of Pseudocalymma alliaceum (Bignoniaceae, Solanales) growing in Dallas Mountain, Jamaica
    • 5.2 Procedure Maintenance of Strains and Production of Biomass:
  • The strains were maintained on agar in petrie dishes (9 cm) or on slants (see list of Media: PCA and PDA). 44 of the strains were grown in shake flasks under the following growth conditions: general fungal media as PC, PD and PB 9 or YPG (see list of media); incubation time from 3 to 9 days, temperature 26° C.; rpm between 150 and 175. Strain No 14 (F. poae) was grown on wheat bran for 15 days (26° C.; static) Strain No 38 was grown in dilute salt solution (DS/2), added 1 cm2 pieces of autoclaved filter paper.
    • Activity Test:
  • Activity was tested on 0.1% AZCL-HE-Cellulose (Megazyme) plates (14 cm Petrie dishes), made up in 1% agarose (HSB, Litex Agarose, Medinova). All tests were done in triplicate, viz. AZCL-HE-Cellulose dissolved in three buffers, adjusted to pH 3, 7 or 9.5 (using various proportions of the following two ingredients Citric acid monohydrate, Merck art. No 100244 (21.0 g) dissolved in water, making a total of 1000 ml, 0.1M tri-Sodium dodecabrohydrate, Merck art. no. 6578 (38 g), dissolved in water, making a total of 1000 ml. The mixing is done immediately before use.
    • Harvesting of Biomass:
  • The biomass was harvested by filtering (mesh adjusted to the growth of the fungus, the finest used for fungi which have highly sporulating mycelium as, e.g., Fusarium spp.) The biomass on the filter was scraped into a sterile plastic bag and immediately frozen (by submerging into liquid nitrogen).
    • 5.3 Results
  • I. Using the PCR screening and amplification techniques described in Materials and Methods the following partial cDNA sequences were obtained:
  • Saccobolus dilutellus (Fuck) Sacc., CBS 275.96: SEQ ID NO: 27 (and the deduced amino acid sequence in SEQ ID NO: 28);
  • Thermomyces verrucosus, CBS 285.96; SEQ ID NO: 29 (and the deduced amino acid sequence in SEQ ID NO: 30);
  • Xylaria hypoxylon, CBS 284.96 SEQ ID NO: 31 (and the deduced amino acid sequence in SEQ ID NO: 32);
  • Fusarium oxysporum ssp lycopersici, CBS 645.78: SEQ ID NO: 33 (and the deduced amino acid sequence in SEQ ID NO: 34);
  • Nectria pinea, CBS 279.96: SEQ ID NO: 35 (and the deduced amino acid sequence in SEQ ID NO:36);
  • Humicola grisea, ATCC 22726: SEQ ID NO: 37 (and the deduced amino acid sequence in SEQ ID NO: 38);
  • Humicola nigrescens CBS 819.73 SEQ ID NO: 39 (and the deduced amino acid sequence in SEQ ID NO: 40);
  • Cladorrhinum foecundissimum ATCC 62373: SEQ ID NO: 41 (and the deduced amino acid sequence in SEQ ID NO: 42);
  • Syspastospora boninensis, NKBC 1515: SEQ ID NO: 43 (and the deduced amino acid sequence in SEQ ID NO: 44):
  • Nigrospora sp., CBS 272.96: SEQ ID NO: 45 (and the deduced amino acid sequence in SEQ ID NO: 46);
  • Chaetostylum fresenii: SEQ ID NO: 47 (and the deduced amino acid sequence in SEQ ID NO: 48);
  • Exidia glandulosa, CBS 277.96: SEQ ID NO: 49 (and the deduced amino acid sequence in SEQ ID NO: 50);
  • Coniothecium sp.: SEQ ID NO: 51 (and the deduced amino acid sequence in SEQ ID NO: 52);
  • Deposition No. CBS 271.96: SEQ ID NO: 53 (and the deduced amino acid sequence in SEQ ID NO: 54);
  • Deposition No, CBS 270.96: SEQ ID NO: 55 (and the deduced amino acid sequence in SEQ ID NO: 56);
  • Diplodia gossypina, CBS 274.96: SEQ ID NO: 57 (and the deduced amino acid sequence in SEQ ID NO: 58);
  • Ulospora bilgramii, NKBC 1444: SEQ ID NO: 59 (and the deduced amino acid sequence in SEQ ID NO: 60);
  • Penicillium verruculosum, ATCC 62396: SEQ ID NO: 61 (and the deduced amino acid sequence in SEQ ID NO: 62);
  • Poronia punctata: SEQ ID NO: 63 (and the deduced amino acid sequence in SEQ ID NO: 64);
  • Fusarium anguioides, IFO 4467 SEQ ID NO: 65 (and the deduced amino acid sequence in SEQ ID NO: 6);
  • Thielavia thermophila, CBS 174.70: SEQ ID NO: 67 (and the deduced amino acid sequence in SEQ ID NO: 68);
  • Chaetomium cuniculorum, CBS 799.83: SEQ ID NO: 69 (and the deduced amino acid sequence in SEQ ID NO: 70);
  • Chaetomium virescens: SEQ ID NO: 71 (and the deduced amino acid sequence in SEQ ID NO: 72);
  • Colletotrichum lagenarium: SEQ ID NO: 73 (and the deduced amino acid sequence in SEQ ID NO: 74);
  • Phycomyces nitens: SEQ ID NO: 75 (and the deduced amino acid sequence in SEQ ID NO: 76); and
  • Trichothecium roseum: SEQ ID NO: 77 (and the deduced amino acid sequence in SEQ ID NO: 78);
  • II. Using the PCR screening and amplification techniques described in Materials and Methods partial cDNA encoding partially for the enzyme of the invention was obtained and the plasmid was deposited according to the Budapest Treaty:
  • Escherichia coli, DSM 10583, deposition date 13 Mar. 1996;
    cDNA from Trichothecium roseum;
    Escherichia coli, DSM 10584, deposition date 13 Mar. 1996,
    cDNA from Syspastospora boninensis;
    Escherichia coli, DSM 10585, deposition date 13 Mar. 1996;
    cDNA from Cheatomium murorum;
    Escherichia coli, DSM 10587, deposition date 13 Mar. 1996;
    cDNA from Sordaria fimicola;
    Escherichia coli, DSM 10588, deposition date 13 Mar. 1996;
    cDNA from the unidentified strain CBS 273.96;
    Escherichia coli, DSM 10586, deposition date 13 Mar. 1996;
    cDNA from Spongipeillis sp.
    • Color Clarification of Crude Supernatants
  • During normal wash the fabric will often fade. However, the fabric appearance is improved and the original colours are much better preserved or maintained if the fabric is washed with a cellulase giving color clarification. Color clarification is measured as removal of surface fibrils and fibers protruding from the yarn of a textile containing cellulosic fibers.
  •
    Apparatus Terg-o-tometer
    Liquid volume 100 ml
    Agitation
    150 movements/min with vertical stirrer
    Rinse time 5 min in tapwater
    Washing temp 40°
    Washing liqour 0.05 M phosphate buffer
    pH 7.0
    Washing time 30 min
    Repetitions
    2
    Enzymes Crude supernatants from the strains shown below.
    Dosage Two dosages from 200, 500, 1000 or 2500 S-CEVU/l
    Textile
    2 swatches of aged black 100% cotton 5 × 6 cm
    (0.9 gram)
    Drying Tumble dry
    • Evaluation:
  • The light remission is measured by a Datacolor Elrepho Remission spectrophotometer. Remission is calculated as delta L (Hunter Lab-values). When the surface fibrins and fibers protruding from the yarn are removed by the cellulase, the surface of the black fabric appears darker, and lower L values are obtained.
  • The samples are compared with a blind sample, i.e., washed without enzyme. Below is shown the delta L remission values compared to a blind sample.
    • REFERENCES Background of the Invention
    • 1. GB-A-1368599
    • 2. EP-A-0 307 564
    • 3. EP-A-0 435 876
    • 4. WO 91/17243
    • 5. WO 91/10732
    • 6. WO 91/17244
    • 7. WO 95/24471
    • 8. WO 95/26398
    • 9. Methods in Enzymology, 1988, Vol. 160, p. 200-391 (edited by Wood, W. A. and Kellogg, S. T.).
    • 10. Béguin. P., “Molecular Biology of Cellulose Degradation”, Annu. Rev. Microbiol. (1990), Vol. 44, pp. 219-248.
    • 11. Henrissat, B., “Cellulases and their interaction with cellulose”, Cellulose (1994), Vol. 1, pp. 169-196.
    • 12. T.-M. Enveri, “Microbial Cellulases” in W. M. Fogarty, Microbial Enzymes and Biotechnology, Applied Science Publishers, 183-224 (1983).
    • 13. Béguin, P. and Aubert, J-P., “The biological degradation of cellulose”, FEMS Microbiology Reviews 13 (1994) 2558.
    • 14. Sheppard, P. O., et al., “The use of conserved cellulase family-specific sequences to clone Cellulase homologue cDNAs from Fusarium oxysporum, Gene, (1994), Vol. 15, pp. 163-167.
    • 15. Saloheimo, A., et al., “A novel, small endoglucanase gene, egl5, from Trichoderma reesei, isolated by expression in yeast”, Molecular Microbiology (1994), Vol. 13(2), pp. 219-228.
    • 16. van Arsdell, J. N. et at., (1987) Cloning, characterization, and expression in Saccharomyces cerevisiae of endoglucanase I from Trichoderma reesei, Bio/Technology 5: 60-64.
    • 17. Penttilä, M. et at, (1986) Homology between cellulase genes of Trichoderma reesei complete nucleotide sequence of the endoglucanase I gene. Gene 45: 253-263.
    • 18. Saloheimo, M. et al, (1988) EGIII, a new endoglucanase from Trichoderma reesei the characterization of both gene and enzyme. Gene 63: 11-21.
    • 19. Gonzáles, R., et at., “Cloning, sequence analysis and yeast expression of the egl1 gene from Trichoderma longibrachiatum”, Appl. Microbiol. Biotechnol., Vol. 38, pp. 370375 (1992).
    • 20. Ooi, T. et at. “Cloning and sequence analysis of a cDNA for cellulase (FI-CMCase) from Aspergillus aculeatus” Curr. Genet., Vol. 18, pp. 217-222 (1990),
    • 21. Ooi, T. et al., “Complete nucleotide sequence of a gene coding for Aspergillus aculeatus cellulase (Fl-CMCase)” Nucleic Acids Research, Vol. 18, No. 19, p. 5884 (1990).
    • 22. Xue, G. et at., “Cloning and expression of multiple cellulase cDNAs from the anaerobic rumen fungus Neocallimastix patriciarum in E. coli, J. Gen. Microbiol., Vol. 138, pp. 1413-1420 (1992).
    • 23. Xue, G. et al., “A novel polysaccharide hydrolase cDNA (celD)) from Neocallimastix patriciarum encoding three multifunctional catalytical domains with high endoglucanase, cellobiohydrolase and xylanase activities”, J. Gen. Microbiol., Vol. 138, pp. 2397-2403 (1992).
    • 24. Zhou, L, et al., “Intronless celB from the anaerobic fungus Neocallimastix patriciarum encodes a modular family A endoglucanase”, Biochem. J., Vol, 297, pp. 359-364 (1994).
    • 25. Dalbøge, H. and Heldt-Hansen, H. P. “A novel method efficient expression cloning of fungal enzyme genes”, Mol. Gen. Genet., Vol. 243, pp. 253-260 (1994).
    • 26. Ali, B. R. S. et al., “Cellulases and hemicellulases of the anaerobic fungus Piromyces constitute a multiprotein cellulose-binding complex and are encoded by multigene families”, FEMS Microbiol. Lett., Vol. 125, No. 1, pp. 15-21 (1995).
    • 27. DNA Data Bank of Japan (DDBJ).
    • 28. Wang, H. Y. and Jones, R. W.: “Cloning, characterization and functional expression of an endoglucanase-encoding gene from the phytopathogenic fungus Macrophomina phaseolina”, Gene, 158:125-128, 1995.
    • 29. Wang, H. Y. and Jones, R. W.: “A unique endoglucanase-encoding gene cloned from the phytopathogenic fungus Macrophomina phaseolina”, Appl. and Environm. Microbiology, 61:2004-2006, 1995.
    • 30. B. Henrissat: Biochem. J., 280: 309, 316, 1991.
    • 31. Schauwecker, F., Wanner, G., Kahmann, R.: “Filament-specific expression of a cellulase gene in the dimorphic fungus Ustilago maydis”, 1995, Biological Chemistry Hoppe-Seyler, 376:617-625.
    • 32. WO 93/20193
    • 33. WO 94/21801
    • 34. WO 94/26880
    • 35. WO 95/02043
    THE DRAWINGS
    • 1. Feng and Doolittle, 1987, J. Mol. Evol. 25: 351-360.
    • 2. NIH Data Base (Entrez, version spring 1996) available on internet.
    • 3. Eriksson, O. E. & Hawksworth, D. L.: Systema Ascomycetum vol 12 (1993).
    • 4, Jülich, W.: Higher Taxa of Basidiomycetes, Bibliotheca Mycologia 85, 485 pp (1981).
    • 5. O'Donnell, K., Zygomycetes in culture, University of Georgia, US, 257 pp (1979).
    • 6. Hawksworth, D. L., Kirk, P. M., Sutton, B. C. and Pegler, D. N.: Dictionary of the fungi, International to Mycological institute, 616 pp (1995).
    • 7. Von Arx, J. A.: The genera of fungi sporulating in culture, 424 pp (1981).
    DETAILED DESCRIPTION
    • 1. Ford et al., Protein Expression and Purification 2: 95-107, 1991.
    • 2. Cunningham and Wells, Science 244, 1081-1085, 1989.
    • 3. de Vos et at., Science 255: 306-312, 1992.
    • 4. Smith et al., J. Mol. Biol. 224: 899-904, 1992.
    • 5. Wlodaver et al. FEBS Lett 309: 59-64, 1992.
    • 6. Tomme, P. et al. “Cellulose-Binding Domains: Classification and Properties” in “Enzymatic Degradation of Insoluble Carbohydrates”, John N. Saddler and Michael H. Penner (Eds.), ACS Symposium Series, No. 618, 1996.
    • 7. WO 90/00609
    • 8. WO 95/16782
    • 9. Needleman, S. B. and Wunsch, C. D., Journal of Molecular Biology, 48-443-453, 1970.
    • 10. WO 94/14953
    • 11. Sambrook, J., Fritsch, E. F. & Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Lab., Cold Spring Harbor, N.Y.
    • 12. Beaucage and Caruthers, Tetrahedron Letters 22: 1859-1869 (1981).
    • 13. Matthes et at., EMBO Journal 3: 801-805 (1984).
    • 14. U.S. Pat. No. 4,683,202
    • 15. Saiki et at., Science 239: 487-491 (1988).
    • 16. Hitzeman et al., J. Biol. Chem. 255 12073-12080 (1980).
    • 17. Alber and Kawasaki, J. Mol. Appl. Gen. 1 419-434 (1982).
    • 18. Young et at. in Genetic Engineering of Microorganisms for Chemicals (Hollaender et at, eds.), Plenum Press, New York, 1982).
    • 19. U.S. Pat. No. 4,599,311
    • 20. Russell et al., Nature 304: 652-654 (1983).
    • 21. McKnight et al., The EMBO J. 4: 2093-2099 (1985).
    • 22. P. R. Russell, Gene 40, pp. 125-130 (1985).
    • 23. U.S. Pat. No. 4,870,008
    • 24. Hagenbuchle et at., Nature 289, pp. 643-646 (1981),
    • 25. L. A. Valls et al., Cell 48, 1987, pp. 887-897.
    • 26. WO 87/02670
    • 27. M. Egel-Mitani et at., Yeast 6, pp. 127-137 (1990).
    • 28. U.S. Pat. No. 4,546,082
    • 29. EP 16 201
    • 30. EP 123 294
    • 31. EP 123 544
    • 32. EP 163 529
    • 33. WO 89/02463
    • 34. WO 92/11378
    • 35. U.S. Pat. No. 4,599,311
    • 36. U.S. Pat. No. 4,931,373
    • 37. U.S. Pat. No. 4,870,008
    • 38. U.S. Pat. No. 5,037,743
    • 39. U.S. Pat. No. 4,845,075
    • 40. U.S. Pat. No. 4,931,373
    • 41. Gleeson et at., J. Gen. Microbiol. 132, pp. 3459-3465 (1986).
    • 42, U.S. Pat. No. 4,882,279
    • 43. EP 272 277
    • 44. EP 230023
    • 45. Malardier et at., 1989, Gene 78-147-156.
    • 46. WO 93/11249.
    • 47. WO 94/14953.
    • 48. WO 95/02043.
    • 49. Horton, R. M., Hunt, H. D., Ho, S, N., Pullen, J. K., and Pease, L. R., Gene 77, 61-68 (1989)
    • 50. Dalbøge, H., and Heldt-Hansen, H., Mol. Gen. Genet 243, 253-260 (1994)
    • 51. Christensen, T., Wødike, H., Boel, E., Mortensen, S. B., Hjortshøj, K., Thim, L., and Hansen,
    • M. T., Bio/Technology 6, 1419-1422 (1988)
    • 52. Sanger, F., Nicklen, S., and Coulson, A. R., Proc. Nat. Acad. Sci. U.S.A. 74, 5463-5467 (1977).
    • 53. Devereux, J., Haeberli, P., and Smithies, O., Nucleic Acids Res. 12, 387-395 (1984).
    • 54. Becker, D. M. & Guarante, L., Methods Enzymol. 194; 182-187 (1991).
    • 55. Gubler, U. & Hoffman, B. J., Gene 25: 263-269 (1983).
    • 56. R. Higuchi, B. Krummel, and R. K. Saiki, A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions, Nucl. Acids Res. 16: 7351-7367 (1988).
    • 57, Sanger, F., Nicklen, S. & Coulson, A. R., Proc. Natl. Acad. Sri. U.S.A. 74: 5463-5467 (1977).
    • 58. N. Axelsen et al., A Manual of Quantitative Immunoelectrophoresis, Blackwell Scientific Publications, 1973, Chapters 2, 3, 4 and 23.

Claims (20)

1. An enzyme preparation consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence
(SEQ ID NO: 79) Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa 1   2   3   4   5   6   7   8   9   10  11  12 Trp Xaa 13  14
and a second amino acid sequence having the following sequence
Trp Cys Cys Xaa Cys (SEQ ID NO: 80) 1   2   3   4   5
wherein
(a) the amino acid residue at position 3 of the first sequence is Trp, Tyr or Phe;
(b) the amino acid residue at position 4 of the first sequence is Trp, Tyr or Phe;
(c) the amino acid residue at position 8 of the first sequence is Arg, Lys or His; and
(d) the amino acid residues at positions 9, 10, 12 and 14 of the first sequence and at position 4 of the second sequence are independently any of the 20 naturally occurring amino acid residues, provided that, in the first amino acid sequence, (i) when the amino acid residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino acid residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala.
2-10. (canceled)
11. An enzyme preparation consisting essentially of an enzyme having cellulytic activity and being obtainable from a strain belonging to Hymenomycetes (Basidiomycota) which enzyme comprises an amino acid sequence selected from the group consisting of the sequences
Xaa Thr Arg Xaa Phe Asp Xaa (SEQ ID NO: 105) 1   2   3   4   5   6   7; Xaa Thr Arg Xaa Tyr Asp Xaa (SEQ ID NO: 106) 1   2   3   4   5   6   7; and Xaa Thr Arg Xaa Trp Asp Xaa (SEQ ID NO: 107) 1   2   3   4   5   6   7
wherein
(a) Xaa at position 4 is Trp, Tyr or Phe; and
(b) Xaa at positions 1 and 7 is independently any of the 20 naturally occurring amino acid residues.
12-65. (canceled)
66. A DNA construct comprising a DNA sequence encoding an enzyme exhibiting cellulytic activity, which DNA sequence comprises
(a) the DNA sequence of SEQ ID NO: 1, and/or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae DSM 9770, or
(b) an analogue of the DNA sequence of SEQ ID NO: 1 or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae DSM 9770, which
(i) is homologous, preferably at least 70% homologous, with the DNA sequence of SEQ ID NO: 1 and/or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae DSM 9770,
(ii) hybridizes under the conditions described herein with the same nucleotide probe as the DNA sequence of SEQ ID NO: 1 and/or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae DSM 9770,
(iii) encodes a polypeptide which is homologous preferably at least 65% homologous, with the polypeptide encoded by a DNA sequence comprising the DNA sequence of SEQ ID NO: 1 and/or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae DSM 9770,
(iv) encodes a polypeptide which is immunologically reactive with an antibody raised against the purified endoglucanase encoded by the DNA sequence of SEQ ID NO: 1 or obtainable from the plasmid in Saccharomyces cerevisiae, DSM 9770.
67. The DNA construct of claim 66, in which the DNA sequence is isolated from or produced on the basis of a DNA library of a strain belonging to the family Chaetomiaceae, preferably to the genus Myceliophthora, in particular a strain of M. thermophila, especially M. thermophila, CBS 117.65.
68-83. (canceled)
84. A recombinant expression vector comprising a DNA construct of claim 66.
85. A cell comprising a DNA construct of claim 66.
86. A cell comprising a recombinant expression vector of claim 84.
87-88. (canceled)
89. A method of producing an enzyme exhibiting endoglucanase activity, comprising culturing a cell of claim 85 under conditions permitting the production of the enzyme, and recovering the enzyme from the culture.
90. (canceled)
91. A method of providing colour clarification of laundry, which method comprising treating the laundry with a soaking, washing or rinsing liquor comprising an enzyme preparation of claim 1.
92-96. (canceled)
97. A laundry composition comprising the enzyme preparation of claim 1, and a compound selected from the group consisting of a surfactant, a builder compound, and a fabric softening agent.
98. The laundry composition of claim 97, which further comprises one or more enzymes selected from the group consisting of proteases, amylases, lipases, cellulases, xylanases, peroxidases and laccases.
99. The composition of claim 97, wherein the surfactant is a nonionic, anionic, cationic, zwitterionic, ampholytic or amphoteric surfactant.
100. The composition of claim 97, wherein the fabric softening agent is a cationic or nonionic softening agent, preferably a quaternary ammonium compound, and which optionally further comprises one or more compounds selected from a surfactant an electrolyte, a buffer, an antioxidant and a liquid carrier.
101-105. (canceled)
US11/740,076 1995-03-17 2007-04-25 Endoglucanases Abandoned US20080145912A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US11/740,076 US20080145912A1 (en) 1995-03-17 2007-04-25 Endoglucanases
US12/567,302 US8642730B2 (en) 1995-03-17 2009-09-25 Endoglucanases
US13/683,165 US9023620B2 (en) 1995-03-17 2012-11-21 Method of providing color clarification of laundry

Applications Claiming Priority (18)

Application Number Priority Date Filing Date Title
DK27295 1995-03-17
DK0272/95 1995-03-17
DK88695 1995-08-08
DK88895 1995-08-08
DK0887/95 1995-08-08
DK88795 1995-08-08
DK0888/95 1995-08-08
DK0886/95 1995-08-08
DK0885/95 1995-08-08
DK88595 1995-08-08
DK13796 1996-02-12
DK0137/96 1996-02-12
PCT/DK1996/000105 WO1996029397A1 (en) 1995-03-17 1996-03-18 Novel endoglucanases
US08/651,136 US6001639A (en) 1995-03-17 1996-05-21 Endoglucanases
US09/229,911 US6387690B1 (en) 1995-03-17 1999-01-13 Endoglucanases
US10/007,521 US6855531B2 (en) 1995-03-17 2001-12-10 Endoglucanases
US10/965,499 US7226773B2 (en) 1995-03-17 2004-10-14 Endoglucanases
US11/740,076 US20080145912A1 (en) 1995-03-17 2007-04-25 Endoglucanases

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US10/965,499 Continuation US7226773B2 (en) 1995-03-17 2004-10-14 Endoglucanases

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/567,302 Division US8642730B2 (en) 1995-03-17 2009-09-25 Endoglucanases

Publications (1)

Publication Number Publication Date
US20080145912A1 true US20080145912A1 (en) 2008-06-19

Family

ID=27545143

Family Applications (7)

Application Number Title Priority Date Filing Date
US08/651,136 Expired - Lifetime US6001639A (en) 1995-03-17 1996-05-21 Endoglucanases
US09/229,911 Expired - Lifetime US6387690B1 (en) 1995-03-17 1999-01-13 Endoglucanases
US10/007,521 Expired - Fee Related US6855531B2 (en) 1995-03-17 2001-12-10 Endoglucanases
US10/965,499 Expired - Fee Related US7226773B2 (en) 1995-03-17 2004-10-14 Endoglucanases
US11/740,076 Abandoned US20080145912A1 (en) 1995-03-17 2007-04-25 Endoglucanases
US12/567,302 Expired - Fee Related US8642730B2 (en) 1995-03-17 2009-09-25 Endoglucanases
US13/683,165 Expired - Fee Related US9023620B2 (en) 1995-03-17 2012-11-21 Method of providing color clarification of laundry

Family Applications Before (4)

Application Number Title Priority Date Filing Date
US08/651,136 Expired - Lifetime US6001639A (en) 1995-03-17 1996-05-21 Endoglucanases
US09/229,911 Expired - Lifetime US6387690B1 (en) 1995-03-17 1999-01-13 Endoglucanases
US10/007,521 Expired - Fee Related US6855531B2 (en) 1995-03-17 2001-12-10 Endoglucanases
US10/965,499 Expired - Fee Related US7226773B2 (en) 1995-03-17 2004-10-14 Endoglucanases

Family Applications After (2)

Application Number Title Priority Date Filing Date
US12/567,302 Expired - Fee Related US8642730B2 (en) 1995-03-17 2009-09-25 Endoglucanases
US13/683,165 Expired - Fee Related US9023620B2 (en) 1995-03-17 2012-11-21 Method of providing color clarification of laundry

Country Status (13)

Country Link
US (7) US6001639A (en)
EP (3) EP1683860B1 (en)
JP (1) JP3360830B2 (en)
CN (5) CN102080070B (en)
AR (1) AR030675A2 (en)
AT (1) ATE315083T1 (en)
AU (1) AU715423B2 (en)
BR (1) BRPI9607646B1 (en)
CA (1) CA2214116C (en)
DE (2) DE69635700T3 (en)
MX (1) MX9706974A (en)
NZ (1) NZ303162A (en)
WO (1) WO1996029397A1 (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090298145A1 (en) * 2008-04-10 2009-12-03 Kabushiki Kaisha Toyota Chuo Kenkyusho Acid-resistance endoglucanase and the use of thereof
WO2013138288A1 (en) 2012-03-16 2013-09-19 Monosol, Llc. Water soluble compositions incorporating enzymes, and method of making same
WO2013158364A1 (en) 2012-04-16 2013-10-24 Monosol, Llc Powdered pouch and method of making same
US8609387B2 (en) 2008-12-30 2013-12-17 Ab Enzymes Oy Fungal endoglucanases, their production and use
US8802423B2 (en) * 2010-12-30 2014-08-12 Novozymes A/S Method for treating textile with endoglucanase
US9850512B2 (en) 2013-03-15 2017-12-26 The Research Foundation For The State University Of New York Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield
US9951363B2 (en) 2014-03-14 2018-04-24 The Research Foundation for the State University of New York College of Environmental Science and Forestry Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects
US11104497B2 (en) 2014-10-03 2021-08-31 Monosol, Llc Degradable materials and packaging made from same

Families Citing this family (786)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102080070B (en) 1995-03-17 2016-01-20 诺沃奇梅兹有限公司 new endoglucanase
US6723549B2 (en) 1995-10-17 2004-04-20 Ab Enzymes Oy Cellulases, the genes encoding them and uses thereof
US6184019B1 (en) 1995-10-17 2001-02-06 Röhm Enzyme Finland OY Cellulases, the genes encoding them and uses thereof
DK0898618T3 (en) 1996-05-10 2008-02-25 Novozymes As Method for providing novel DNA sequences
GB9613758D0 (en) † 1996-07-01 1996-09-04 Unilever Plc Detergent composition
EP1726644A1 (en) * 1996-09-17 2006-11-29 Novozymes A/S Cellulase variants
EP0843041A1 (en) * 1996-11-13 1998-05-20 Novo Nordisk A/S Garments with considerable variation in abrasion level and process for its production using cellulolytic enzymes
FI964691A0 (en) * 1996-11-25 1996-11-25 Primalco Ltd Cellulose weaving process
FI964692A0 (en) * 1996-11-25 1996-11-25 Primalco Ltd Cellulose-based cellulose processing
DE19729323A1 (en) * 1997-07-09 1999-01-14 Wolff Walsrode Ag Process for the production of cellulose derivatives
AU7564198A (en) * 1998-05-01 1999-11-23 Procter & Gamble Company, The Laundry detergent and/or fabric care compositions comprising a modified cellulase
JP4047545B2 (en) 1998-06-10 2008-02-13 ノボザイムス アクティーゼルスカブ New mannanase
DE69942343D1 (en) 1998-10-23 2010-06-17 Meiji Seika Kaisha ENDOGLUKANASE AND CELLULASE CONTAINING PREPARATIONS
EP2716753A1 (en) 1998-11-27 2014-04-09 Novozymes A/S Lipolytic enzyme variants
US6268328B1 (en) * 1998-12-18 2001-07-31 Genencor International, Inc. Variant EGIII-like cellulase compositions
US6579841B1 (en) 1998-12-18 2003-06-17 Genencor International, Inc. Variant EGIII-like cellulase compositions
ATE504651T1 (en) 1998-12-18 2011-04-15 Novozymes As SUBTILASE ENZYMES OF THE I-S1 AND I-S2 SUBGROUPS WITH AN ADDITIONAL AMINO ACID RESIDUE IN AN ACTIVE LOOP REGION
JP2002534593A (en) * 1999-01-14 2002-10-15 ザ、プロクター、エンド、ギャンブル、カンパニー Detergent composition containing pectin-degrading enzyme system
CA2365446C (en) 1999-03-31 2012-07-10 Novozymes A/S Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same
EP1169434B1 (en) 1999-03-31 2009-02-11 Novozymes A/S Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same
EP1183341B2 (en) 1999-05-20 2012-05-02 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 127 and 128
ATE407201T1 (en) 1999-05-20 2008-09-15 Novozymes As SUBTILASE ENZYMES OF THE I-S1 AND I-S2 SUBGROUPS WITH AT LEAST ONE ADDITIONAL AMINO ACID RESIDUE BETWEEN POSITIONS 126 AND 127
WO2000071685A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 132 and 133
AU4392700A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 129 and 130
EP1183342B2 (en) 1999-05-20 2012-06-13 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 128 and 129
DE60039693D1 (en) 1999-05-20 2008-09-11 Novozymes As SUBTILASE ENZYMES OF I-S1 AND I-S2 SUB-GROUPS WITH AT LEAST ONE ADDITIONAL AMINO ACID RESISTANCE BETWEEN POSITIONS 125 AND 126
GB9918492D0 (en) * 1999-08-06 1999-10-06 Thomas & Fontaine Ltd Composting additive
EP2336331A1 (en) 1999-08-31 2011-06-22 Novozymes A/S Novel proteases and variants thereof
AU7405300A (en) * 1999-10-01 2001-05-10 Novozymes A/S Cellulose films for screening
DK1272643T4 (en) * 2000-03-20 2014-05-26 Basf Se Talaromyces emersonii beta-glucanases
AU2001258799A1 (en) * 2000-05-22 2001-12-03 Meiji Seika Kaisha Ltd. Endoglucanase nce5 and cellulase preparations containing the same
CA2413838A1 (en) * 2000-06-02 2001-12-06 Novozymes A/S Redeposition or backstain inhibition during stonewashing process
AR030462A1 (en) 2000-08-21 2003-08-20 Novozymes As NOVEDOS SUBTILASAS ENZYMES THAT HAVE A REDUCED TREND TOWARDS THEIR INHIBITION FOR SUBSTANCES PRESENT IN EGGS
EP1975229A3 (en) 2000-10-13 2009-03-18 Novozymes A/S Alpha-amylase variant with altered properties
AU2002210379A1 (en) 2000-10-13 2002-04-22 Novozymes A/S Subtilase variants
EP1423513B1 (en) 2001-05-15 2009-11-25 Novozymes A/S Alpha-amylase variant with altered properties
US20030051836A1 (en) * 2001-05-21 2003-03-20 Novozymes A/S Enzymatic hydrolysis of a polymer comprising vinyl acetate monomer
EP2298868B1 (en) 2001-06-26 2015-01-07 Novozymes A/S Polypeptides having cellobiohydrolase I activity and polynucleotides encoding same
DK200101090A (en) 2001-07-12 2001-08-16 Novozymes As Subtilase variants
US7063895B2 (en) * 2001-08-01 2006-06-20 National Starch And Chemical Investment Holding Corporation Hydrophobically modified solution polymers and their use in surface protecting formulations
US20050258095A1 (en) * 2002-01-29 2005-11-24 Jawed Sarkar Enzyme-assisted clarification and dewatering of wastewater
EP1490485B1 (en) 2002-03-27 2015-03-04 Novozymes A/S Granules with filamentous coatings
WO2003089598A2 (en) * 2002-04-19 2003-10-30 Novozymes Biotech, Inc Polypeptides having xyloglucanase activity and nucleic acids encoding same
ATE420161T1 (en) 2002-07-01 2009-01-15 Novozymes As MONOPROPYLENE GLYCOL AS A FERMENTATION ADDITIVE
SE525872C2 (en) * 2002-09-06 2005-05-17 Stora Enso Ab Method of producing mechanical pulp with reduced energy consumption
EP2302046B1 (en) 2002-10-01 2012-01-04 Novozymes A/S Family GH 61 polypeptides
DE60325269D1 (en) * 2002-10-31 2009-01-22 Meiji Seika Kaisha NEW, AGAINST TENSID TOLERANT CELLULASE
TWI319007B (en) 2002-11-06 2010-01-01 Novozymes As Subtilase variants
WO2004053039A2 (en) 2002-12-11 2004-06-24 Novozymes A/S Detergent composition comprising endo-glucanase
DE10260903A1 (en) 2002-12-20 2004-07-08 Henkel Kgaa New perhydrolases
AU2003291962A1 (en) 2002-12-20 2004-07-14 Novozymes A/S Polypeptides having cellobiohydrolase ii activity and polynucleotides encoding same
EP1579057A4 (en) * 2002-12-23 2007-08-01 Novozymes North America Inc A method of treating polyester fabrics
ATE461276T1 (en) 2003-01-27 2010-04-15 Novozymes As ENZYME STABILIZATION
WO2004074419A2 (en) 2003-02-18 2004-09-02 Novozymes A/S Detergent compositions
TW593837B (en) * 2003-02-26 2004-06-21 Yuen Foong Yu Paper Mfg Co Ltd Biopulping method for plant fiber
TW592629B (en) * 2003-02-26 2004-06-21 Yuen Foong Yu Paper Mfg Co Ltd The manufacturing method for a plant fiber mulching mat
DK1622921T3 (en) 2003-05-02 2010-09-13 Novozymes Inc Variants of beta-glucosidases
CA2538349C (en) 2003-06-25 2014-08-12 Novozymes A/S Polypeptides having alpha-amylase activity and polynucleotides encoding same
CN108486086A (en) 2003-07-02 2018-09-04 维莱尼姆公司 Dextranase, encode they nucleic acid and preparation and use their method
US7741089B2 (en) 2003-08-11 2010-06-22 Verenium Corporation Laccases, nucleic acids encoding them and methods for making and using them
ES2538824T3 (en) 2003-08-25 2015-06-24 Novozymes, Inc. Variants of glycoside hydrolases
US20050054752A1 (en) * 2003-09-08 2005-03-10 O'brien John P. Peptide-based diblock and triblock dispersants and diblock polymers
US7892808B2 (en) 2003-10-10 2011-02-22 Norozymes A/S Protease variants
JP4880469B2 (en) 2003-10-23 2012-02-22 ノボザイムス アクティーゼルスカブ Protease with improved stability in detergents
ES2437198T3 (en) 2003-10-28 2014-01-09 Novozymes Inc. Polypeptides with beta-glucosidase activity and isolated polynucleotides encoding the polypeptides
EP1700917B1 (en) 2003-12-03 2016-04-13 Meiji Seika Pharma Co., Ltd. Endoglucanase stce and cellulase preparation containing the same
DE10360805A1 (en) 2003-12-23 2005-07-28 Henkel Kgaa New alkaline protease and detergents containing this novel alkaline protease
JP5059412B2 (en) 2004-01-06 2012-10-24 ノボザイムス アクティーゼルスカブ Alicyclobacillus sp. Polypeptide
WO2005067531A2 (en) * 2004-01-16 2005-07-28 Novozymes Inc. Methods for degrading lignocellulosic materials
DK2305702T3 (en) 2004-01-30 2014-06-16 Novozymes Inc Polypeptides with cellulolytic enhancing activity and polynucleotides encoding them
EP1713919A2 (en) 2004-02-13 2006-10-25 Novozymes A/S Protease variants
US7148404B2 (en) 2004-05-04 2006-12-12 Novozymes A/S Antimicrobial polypeptides
ATE541034T1 (en) 2004-06-21 2012-01-15 Novozymes As NOCARDIOPSIS PROTEASES
DK1781790T3 (en) 2004-07-05 2016-01-18 Novozymes As ALFA-amylase variants WITH CHANGED PROPERTIES
DE602005026541D1 (en) 2004-09-21 2011-04-07 Novozymes As SUBTILASES
ATE533843T1 (en) 2004-09-21 2011-12-15 Novozymes As SUBTILATE
WO2006032278A1 (en) 2004-09-21 2006-03-30 Novozymes A/S Subtilases
EP1799819B1 (en) 2004-09-30 2011-03-23 Novozymes Inc. Polypeptides having lipase activity and polynucleotides encoding same
DE102004047776B4 (en) 2004-10-01 2018-05-09 Basf Se Stabilized against di- and / or multimerization alpha-amylase variants, processes for their preparation and their use
DE102004047777B4 (en) 2004-10-01 2018-05-09 Basf Se Alpha-amylase variants with increased solvent stability, process for their preparation and their use
GB0425102D0 (en) * 2004-11-15 2004-12-15 Ciba Spec Chem Water Treat Ltd Polymeric compositions and methods of employing them in papermaking processes
CN101253263B (en) 2005-04-27 2014-07-02 诺维信股份有限公司 Polypeptides having endoglucanase activity and polynucleotides encoding same
US7741093B2 (en) 2005-04-29 2010-06-22 Ab Enzymes Oy Cellulases and their uses
BRPI0609906A8 (en) 2005-04-29 2017-11-21 Ab Enzymes Oy cellulase fusion protein, its production process and detergent composition containing it, expression vector, host cell, enzyme preparation, fading and bio-finishing processes and methods of treating cellulosic fiber containing textile material to treat fiber or pulp derived from wood and to improve the quality of animal feed
EP1904628B1 (en) 2005-07-08 2011-10-19 Novozymes A/S Subtilase variants
ATE530642T1 (en) 2005-08-16 2011-11-15 Novozymes As SUBTILATE
CN101243181A (en) 2005-08-16 2008-08-13 诺维信公司 Polypeptides of strain bacillus sp. P203
EP2385110A3 (en) 2005-09-30 2011-11-16 Novozymes, Inc. Methods for enhancing the degradation or conversion of cellulosic material
WO2007036235A1 (en) 2005-09-30 2007-04-05 Novozymes A/S Immobilization of enzymes
DE102005053529A1 (en) 2005-11-08 2007-06-21 Henkel Kgaa System for the enzymatic generation of hydrogen peroxide
FI119325B (en) * 2005-12-22 2008-10-15 Ab Enzymes Oy New endoglucanase polypeptides and their preparation and use
US7256032B2 (en) 2005-12-22 2007-08-14 Ab Enzymes Oy Enzymes
FI120045B (en) 2005-12-22 2009-06-15 Roal Oy Treatment of cellulose materials and enzymes useful therein
ES2637008T3 (en) * 2005-12-22 2017-10-10 Ab Enzymes Oy New enzymes
DE102006038448A1 (en) 2005-12-28 2008-02-21 Henkel Kgaa Enzyme-containing cleaning agent
WO2007098756A1 (en) 2006-03-02 2007-09-07 Novozymes A/S High capacity encapsulation process
US7883711B2 (en) 2006-03-22 2011-02-08 Novozymes A/S Use of polypeptides having antimicrobial activity
US8080386B2 (en) 2006-03-30 2011-12-20 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
BRPI0710218A2 (en) 2006-03-30 2011-08-02 Novozymes Inc isolated polypeptide, polynucleotide, nucleic acid construct, recombinant expression vector, recombinant host cell, methods for making polypeptide, for producing a mutant from a precursor cell, for producing a protein, for producing a polynucleotide, for degrading or converting a cellulosic material, to produce a substance and to inhibit expression of a polypeptide in a cell, mutant cell, transgenic plant, plant part or plant cell, and double stranded rna molecule
EP2004789B1 (en) 2006-03-31 2012-08-29 Novozymes A/S A stabilized liquid enzyme composition
US7361487B2 (en) 2006-04-13 2008-04-22 Ab Enzymes Oy Enzyme fusion proteins and their use
FI119434B (en) * 2006-04-13 2008-11-14 Ab Enzymes Oy Enzyme fusion proteins and their use
WO2007118935A1 (en) * 2006-04-13 2007-10-25 Ab Enzymes Oy Enzyme fusion proteins and their use
WO2007149699A2 (en) 2006-06-21 2007-12-27 Novozymes North America, Inc. Desizing and scouring process
BRPI0714870A2 (en) 2006-07-21 2013-05-28 Novozymes Inc Method for producing a secreted polypeptide having biological activity, isolated fusion protein, isolated polynucleotide, fusion protein construct, expression vector, authentic host cell, methods for degrading or converting a cellulosic material and for producing a substance
AU2007356171B8 (en) 2006-08-04 2014-01-16 Bp Corporation North America Inc. Glucanases, nucleic acids encoding them, and methods for making and using them
JP5394922B2 (en) 2006-08-11 2014-01-22 ノボザイムス バイオロジカルズ，インコーポレイティド Bacteria culture solution and fungus culture solution-containing composition
JP5497440B2 (en) 2006-10-06 2014-05-21 ノボザイムス アクティーゼルスカブ Detergent composition and combined use of enzymes in the composition
DE102006055669A1 (en) 2006-11-23 2008-07-17 Henkel Kgaa Enzyme preparation with carrier-bound antioxidants
RU2459867C2 (en) 2006-12-21 2012-08-27 ДАНИСКО ЮЭс, ИНК., ДЖЕНЕНКОР ДИВИЖН COMPOSITIONS BASED ON α-AMYLASE POLYPEPTIDE FROM BACILLUS, TYPE 195, AND USE THEREOF
DE102007003143A1 (en) 2007-01-16 2008-07-17 Henkel Kgaa New alkaline protease from Bacillus gibsonii and detergents and cleaners containing this novel alkaline protease
DE102007008655A1 (en) 2007-02-20 2008-08-21 Henkel Ag & Co. Kgaa Siderophore-metal complexes as bleach catalysts
DE102007010785A1 (en) 2007-03-02 2008-09-04 Henkel Ag & Co. Kgaa Use of superoxide dismutases to cleave and/or remove Amadori and/or Maillard products, especially as components of detergent, cosmetic or pharmaceutical products
RU2009137386A (en) 2007-03-09 2011-04-20 ДАНИСКО ЮЭс ИНК., ДЖЕНЕНКОР ДИВИЖН (US) AMILASE VARIANTS OF BACILLUS ALKALYPHIC SPECIES, COMPOSITIONS CONTAINING AMILASE OPTIONS AND METHODS OF APPLICATION
CA2680370C (en) 2007-03-23 2017-03-14 Sarah Mchatton Preventing and reducing biofilm formation and planktonic proliferation
WO2008118377A2 (en) 2007-03-23 2008-10-02 Danisco Us, Inc., Genencor Division Enhanced amylase production by n-terminal addition to mature amylase protein
DE102007016139A1 (en) 2007-03-30 2008-10-02 Jenabios Gmbh Method for regioselective oxygenation of N-heterocycles
DE102007017656A1 (en) 2007-04-12 2008-10-16 Henkel Ag & Co. Kgaa Biheteroaryl metal complexes as bleaching catalysts
DE102007017654A1 (en) 2007-04-12 2008-10-16 Henkel Ag & Co. Kgaa Bis (hydroxyquinoline) metal complexes as bleaching catalysts
DE102007017657A1 (en) 2007-04-12 2008-10-16 Henkel Ag & Co. Kgaa Tris / heterocyclyl) metal complexes as bleach catalysts
SG148934A1 (en) 2007-06-11 2009-01-29 Novozymes As A process for combined biopolishing and bleach clean-up
WO2009010444A2 (en) * 2007-07-13 2009-01-22 Novozymes A/S Biopolishing on man-made cellulosic fabric
DE102007040326A1 (en) 2007-08-24 2009-02-26 Henkel Ag & Co. Kgaa Laundry pre-treatment agent and method
DE102007047433A1 (en) * 2007-10-04 2009-04-09 Lanxess Deutschland Gmbh Liquid washing and liquid cleaning agents
DE102007049830A1 (en) 2007-10-16 2009-04-23 Henkel Ag & Co. Kgaa New protein variants by circular permutation
DE102007051092A1 (en) 2007-10-24 2009-04-30 Henkel Ag & Co. Kgaa Subtilisin from Becillus pumilus and detergents and cleaners containing this new subtilisin
CA2709490A1 (en) 2007-12-19 2009-07-09 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
EP2085070A1 (en) * 2008-01-11 2009-08-05 Procter &amp; Gamble International Operations SA. Cleaning and/or treatment compositions
AR070497A1 (en) * 2008-02-29 2010-04-07 Procter & Gamble DETERGENT COMPOSITION THAT LIPASA INCLUDES
BRPI0909707A2 (en) * 2008-02-29 2015-08-25 Procter & Gamble Detergent composition comprising lipase.
US8082826B2 (en) * 2008-03-25 2011-12-27 Power Tool Institute Saw accessories and clamp for use therewith
EP2283136A2 (en) 2008-04-30 2011-02-16 Danisco US Inc. New chimeric alpha-amylase variants
BRPI0913367A2 (en) 2008-06-06 2015-08-04 Danisco Us Inc Bacillus subtilis variant alpha-amylases and methods of use
MX2010013108A (en) 2008-06-06 2010-12-21 Danisco Inc Production of glucose from starch using alpha-amylases from bacillus subtilis.
EP2291526B1 (en) 2008-06-06 2014-08-13 Danisco US Inc. Saccharification enzyme composition with Bacillus subtilis alpha-amylase
DE102008027375A1 (en) 2008-06-09 2009-12-10 Henkel Ag & Co. Kgaa Bacitracin-metal complexes as bleaching catalysts
GB0810881D0 (en) 2008-06-16 2008-07-23 Unilever Plc Improvements relating to fabric cleaning
ES2430858T3 (en) * 2008-06-20 2013-11-22 The Procter & Gamble Company Composition for laundry
EP2149786A1 (en) 2008-08-01 2010-02-03 Unilever PLC Improvements relating to detergent analysis
US7927856B2 (en) * 2008-08-15 2011-04-19 Academia Sinica Thermophilic endo-glucanase and uses thereof
ES2691384T3 (en) * 2008-09-02 2018-11-27 Basf Se Procedure for manufacturing paper, cardboard and cardboard using endo-beta-1,4-glucanases as a drainage agent
BRMU8903145Y1 (en) 2008-09-12 2017-05-09 Unilever Nv packed wash product
KR101062978B1 (en) 2008-11-12 2011-09-07 건국대학교 산학협력단 Production Optimization and Gene Cloning of Endoglucanases Derived from the New Bacterium Penicillium Pinophilum ＫＭＪ601
EP2358878B1 (en) 2008-11-20 2014-10-15 Novozymes Inc. Polypeptides having amylolytic enhancing activity and polynucleotides encoding same
CN102300986A (en) 2008-12-04 2011-12-28 诺维信股份有限公司 Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same
EP2376527A1 (en) 2008-12-12 2011-10-19 Novozymes Inc. Polypeptides having lipase activity and polynucleotides encoding same
US8338121B2 (en) 2008-12-19 2012-12-25 Novozymes, Inc. Methods for determining cellulolytic enhancing activity of a polypeptide
US8426158B2 (en) 2008-12-19 2013-04-23 Novozymes, Inc. Methods for increasing enzymatic hydrolysis of cellulosic material in the presence of a peroxidase
US8337663B2 (en) 2008-12-19 2012-12-25 Novozymes, Inc. Methods for increasing hydrolysis of cellulosic material
CN102325879A (en) 2008-12-19 2012-01-18 诺维信股份有限公司 Methods for increasing hydrolysis of cellulosic material in the presence of cellobiose dehydrogenase
EP2202290A1 (en) 2008-12-23 2010-06-30 Unilever PLC A flowable laundry composition and packaging therefor
WO2010088387A1 (en) 2009-01-28 2010-08-05 Novozymes, Inc. Polypeptides having beta-glucosidase activity and polynucleotides encoding same
WO2010088463A2 (en) 2009-01-30 2010-08-05 Novozymes, Inc. Polypeptides having expansin activity and polynucleotides encoding same
WO2010097436A1 (en) 2009-02-27 2010-09-02 Novozymes A/S Mutant cells having reduced expression of metallopeptidase, suitable for recombinant polypeptide production
US20120172275A1 (en) 2009-03-10 2012-07-05 Danisco Us Inc. Bacillus Megaterium Strain DSM90-Related Alpha-Amylases, and Methods of Use, Thereof
EP2411511B1 (en) 2009-03-24 2018-08-08 Novozymes A/S Polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
US8088608B2 (en) * 2009-03-31 2012-01-03 Codexis, Inc. Endoglucanases
BRPI1012590A2 (en) 2009-04-08 2015-09-22 Danisco Us Inc Genencor Div halomonas strain wdg-195-related alpha-amylases and methods of using them
US8439980B2 (en) 2009-05-05 2013-05-14 Conopco, Inc. Shading composition
MX2011012585A (en) 2009-05-29 2011-12-14 Novozymes Inc Methods for enhancing the degradation or conversion of cellulosic material.
CN104480088A (en) 2009-06-02 2015-04-01 诺维信股份有限公司 Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
US20100316764A1 (en) * 2009-06-10 2010-12-16 Engrain, LLC Flour supplement compositions and methods for preparing wheat flour
EP2451957B1 (en) 2009-07-07 2017-11-15 Novozymes Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CN102471738B (en) 2009-07-09 2015-11-25 宝洁公司 Comprise the low composite solid fabric process detergent composition of slight alkalescence of phthalimido peroxy caproic acid
WO2011005911A1 (en) 2009-07-09 2011-01-13 The Procter & Gamble Company Method of laundering fabric using a compacted liquid laundry detergent composition
WO2011005913A1 (en) 2009-07-09 2011-01-13 The Procter & Gamble Company A catalytic laundry detergent composition comprising relatively low levels of water-soluble electrolyte
EP2451915A1 (en) 2009-07-09 2012-05-16 The Procter & Gamble Company A catalytic laundry detergent composition comprising relatively low levels of water-soluble electrolyte
HUE029942T2 (en) 2009-08-13 2017-04-28 Procter & Gamble Method of laundering fabrics at low temperature
EP3805348A3 (en) 2009-09-17 2021-07-14 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2011035029A1 (en) 2009-09-18 2011-03-24 Novozymes, Inc. Polypeptides having beta-glucosidase activity and polynucleotides encoding same
EP3296394B1 (en) 2009-09-23 2020-11-04 Danisco US Inc. Novel glycosyl hydrolase enzymes and uses thereof
AU2010299799B2 (en) 2009-09-25 2015-10-29 Novozymes A/S Subtilase variants
RU2639534C2 (en) 2009-09-25 2017-12-21 Новозимс А/С Application of protease versions
WO2011041397A1 (en) 2009-09-29 2011-04-07 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US8211665B2 (en) 2009-09-29 2012-07-03 Novozymes, Inc. Polypeptides having xylanase activity and polynucleotides encoding same
EP2977382A3 (en) 2009-09-30 2016-05-11 Novozymes Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2011039319A1 (en) 2009-09-30 2011-04-07 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
MX2012004091A (en) 2009-10-08 2012-04-20 Unilever Nv Shading composition.
BR112012007757B1 (en) 2009-10-13 2019-08-27 Unilever Nv fabric wash treatment composition and domestic method of fabric treatment
MX311859B (en) 2009-10-23 2013-07-31 Unilever Nv Dye polymers.
CN102597228A (en) 2009-10-23 2012-07-18 诺维信股份有限公司 Cellobiohydrolase variants and polynucleotides encoding same
MX356389B (en) 2009-10-23 2018-05-28 Danisco Us Inc Methods for reducing blue saccharide.
CA2776336A1 (en) 2009-10-29 2011-05-19 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
WO2011057083A1 (en) 2009-11-06 2011-05-12 Novozymes, Inc. Polypeptides having xylanase activity and polynucleotides encoding same
WO2011057086A1 (en) 2009-11-06 2011-05-12 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
EP2516663B1 (en) 2009-12-23 2020-02-12 Danisco US Inc. Methods for improving the efficiency of simultaneous saccharification and fermentation reactions
WO2011080267A2 (en) 2009-12-29 2011-07-07 Novozymes A/S Polypetides having detergency enhancing effect
WO2011082889A1 (en) 2010-01-07 2011-07-14 Unilever Plc Natural shading agents
JP2013517762A (en) 2010-01-22 2013-05-20 デュポン ニュートリション バイオサイエンシーズ エーピーエス Process for producing amino-substituted glycolipid compounds
ES2477518T3 (en) 2010-02-09 2014-07-17 Unilever Nv Coloring polymers
EP2357220A1 (en) 2010-02-10 2011-08-17 The Procter & Gamble Company Cleaning composition comprising amylase variants with high stability in the presence of a chelating agent
CN113186178A (en) 2010-02-10 2021-07-30 诺维信公司 Variants and compositions comprising variants with high stability in the presence of chelating agents
WO2011098356A1 (en) 2010-02-12 2011-08-18 Unilever Plc Laundry treatment composition comprising bis-azo shading dyes
US8859259B2 (en) 2010-02-14 2014-10-14 Ls9, Inc. Surfactant and cleaning compositions comprising microbially produced branched fatty alcohols
US8815559B2 (en) 2010-02-18 2014-08-26 Danisco Us Inc. Amylase from nesterenkonia and methods of use, thereof
EP2539447B1 (en) 2010-02-25 2017-07-26 Novozymes A/S Variants of a lysozyme and polynucleotides encoding same
EP2365058A1 (en) 2010-03-01 2011-09-14 The Procter & Gamble Company Solid laundry detergent composition having an excellent anti-encrustation profile
EP2365054A1 (en) 2010-03-01 2011-09-14 The Procter & Gamble Company Solid laundry detergent composition comprising secondary alcohol-based detersive surfactant
EP2380957A1 (en) 2010-04-19 2011-10-26 The Procter & Gamble Company Solid laundry detergent composition having a dynamic in-wash ph profile
EP2377914B1 (en) 2010-04-19 2016-11-09 The Procter & Gamble Company Mildly alkaline, low-built, solid fabric treatment detergent composition comprising perhydrolase
EP2363456A1 (en) 2010-03-01 2011-09-07 The Procter & Gamble Company Solid laundry detergent composition comprising brightener in micronized particulate form
EP2365059A1 (en) 2010-03-01 2011-09-14 The Procter & Gamble Company Solid laundry detergent composition comprising C.I. fluorescent brightener 260 in alpha-crystalline form
CA2794963A1 (en) 2010-03-31 2011-10-06 Novozymes, Inc. Cellobiohydrolase variants and polynucleotides encoding same
US20110257069A1 (en) 2010-04-19 2011-10-20 Stephen Joseph Hodson Detergent composition
US20110257060A1 (en) 2010-04-19 2011-10-20 Robert Richard Dykstra Laundry detergent composition comprising bleach particles that are suspended within a continuous liquid phase
US20110257062A1 (en) 2010-04-19 2011-10-20 Robert Richard Dykstra Liquid laundry detergent composition comprising a source of peracid and having a ph profile that is controlled with respect to the pka of the source of peracid
MX2012012096A (en) 2010-04-26 2012-12-17 Novozymes As Enzyme granules.
KR101150280B1 (en) 2010-04-28 2012-05-24 건국대학교 산학협력단 Cellulase producing Nectria cinnabarina and its use for saccharification
CN102892875A (en) 2010-04-29 2013-01-23 荷兰联合利华有限公司 Bis-heterocyclic azo dyes
US20130260438A1 (en) 2010-05-06 2013-10-03 Danisco Us Inc. Compositions and methods comprising serine protease variants (as amended)
CA3152266A1 (en) 2010-06-03 2011-12-08 Lallemand Hungary Liquidity Management Llc Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose
EP2395071A1 (en) 2010-06-10 2011-12-14 The Procter & Gamble Company Solid detergent composition comprising lipase of bacterial origin
ES2394066T3 (en) 2010-06-24 2013-01-16 The Procter & Gamble Company Stable compositions comprising cationic cellulose polymer and cellulase
CN103108951A (en) 2010-06-30 2013-05-15 诺维信公司 Polypeptides having beta-glucosidase activity and polynucleotides encoding same
WO2012021396A1 (en) 2010-08-12 2012-02-16 Novozymes, Inc. Compositions comprising a polypeptide having cellulolytic enhancing activity and an organic compound and uses thereof
CN103189493A (en) 2010-08-30 2013-07-03 诺维信公司 A concentrated soak wash
CN103492545A (en) 2010-08-30 2014-01-01 诺维信公司 A two-soak wash
US20130266554A1 (en) 2010-09-16 2013-10-10 Novozymes A/S Lysozymes
BR112013007693B1 (en) 2010-09-30 2021-12-07 Novozymes, Inc VARIANT, ISOLATED POLYNUCLEOTIDE, RECOMBINANT MICROBIAL HOST CELL, METHODS FOR PRODUCING A VARIANT, TO PRODUCE A VARIANT HAVING CELLULOLYTIC ENHANCEMENT ACTIVITY, TO DEGRADE A CELLULOSIC MATERIAL, TO PRODUCE A FERMENTATION PRODUCT, TO FERMENT A CELLULOSIC, AND ELIGIBLE MATERIAL. COMPLETE BROTH OR CELL CULTURE COMPOSITION
WO2012044836A1 (en) 2010-09-30 2012-04-05 Novozymes, Inc. Variants of polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
EP2627760B1 (en) 2010-10-14 2016-08-10 Unilever PLC Laundry detergent particles
ES2537714T3 (en) 2010-10-14 2015-06-11 Unilever N.V. Laundry detergent particles
ES2529715T3 (en) 2010-10-14 2015-02-25 Unilever N.V. Particulate detergent compositions comprising fluorescent agent
CN103282477B (en) 2010-10-14 2015-04-01 荷兰联合利华有限公司 Top-loading laundry vessel method
AU2011315793B2 (en) 2010-10-14 2014-03-06 Unilever Plc Laundry detergent particles
WO2012049034A1 (en) 2010-10-14 2012-04-19 Unilever Plc Packaging and dispensing of detergent compositions
CN103154228B (en) 2010-10-14 2015-04-08 荷兰联合利华有限公司 Laundry detergent particles
EP2441825A1 (en) 2010-10-14 2012-04-18 Unilever Plc, A Company Registered In England And Wales under company no. 41424 of Unilever House Process for preparing laundry detergent particles
MX342221B (en) 2010-10-14 2016-09-21 Unilever N V * Packaged particulate detergent composition.
CN103153813B (en) 2010-10-14 2015-08-19 荷兰联合利华有限公司 Comprise the packaging of laundry composition, described packaging divider and utilize described divider and described packaging to carry out the method for washing
EP2441822A1 (en) 2010-10-14 2012-04-18 Unilever Plc, A Company Registered In England And Wales under company no. 41424 of Unilever House Laundry detergent particles
MX340443B (en) 2010-10-14 2016-07-08 Unilever N V * Packaged particulate detergent composition.
EP2441820A1 (en) 2010-10-14 2012-04-18 Unilever Plc, A Company Registered In England And Wales under company no. 41424 of Unilever House Laundry detergent particles
US9284517B2 (en) 2010-10-14 2016-03-15 Conopco Inc. Laundry detergent particle
MY164216A (en) 2010-10-14 2017-11-30 Unilever Nv Laundry detergent particles
BR112013008954A2 (en) 2010-10-14 2016-06-28 Unilever Nv packaged particulate detergent composition packaged in a pack
BR112013009135B1 (en) 2010-10-14 2021-01-05 Unilever N.V. packed product
IN2013MN00626A (en) 2010-10-14 2015-06-12 Unilever Plc
WO2012049032A1 (en) 2010-10-14 2012-04-19 Unilever Plc Refill and refillable packages of concentrated particulate detergent compositions
BR112013009456B1 (en) 2010-10-22 2021-11-30 Unilever Ip Holdings B.V. STRUCTURED AQUEOUS LIQUID DETERGENT COMPOSITION AND PROCESS TO MANUFACTURE A STRUCTURED AQUEOUS LIQUID DETERGENT
BR112013010129A2 (en) 2010-11-18 2016-07-05 Novozymes Inc isolated chimeric gh61 polypeptide, isolated polynucleotide, host cell, methods for producing a chimeric gh61 polypeptide, for degrading or converting a cellulostic material, for synthesizing a fermentation product, for fermenting a cellulosic material and for cleaning or washing a hard surface or clothing for washing, transgenic plant, part of plant or plant cell, use of chimeric gh61 polypeptide, detergent composition, and broth formulation or whole cell culture composition
EP2649188A1 (en) 2010-12-06 2013-10-16 Novozymes North America, Inc. Methods of hydrolyzing oligomers in hemicellulosic liquor
US9027765B2 (en) 2010-12-17 2015-05-12 Hollingsworth & Vose Company Filter media with fibrillated fibers
DE102010063457A1 (en) 2010-12-17 2012-06-21 Henkel Ag & Co. Kgaa Storage stable liquid washing or cleaning agent containing protease and cellulase
DE102010063743A1 (en) 2010-12-21 2012-06-21 Henkel Ag & Co. Kgaa Liquid surfactant preparation containing lipase and phosphonate
CN103429736A (en) * 2010-12-30 2013-12-04 诺维信公司 Method for treating textile with endoglucanase
WO2012098046A1 (en) 2011-01-17 2012-07-26 Unilever Plc Dye polymer for laundry treatment
DK2668269T3 (en) 2011-01-26 2018-04-09 Novozymes Inc POLYPEPTIDES WITH ENDOGLUCANASE ACTIVITY AND POLYNUCLEOTIDES CODING THEM
WO2012103322A1 (en) 2011-01-26 2012-08-02 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
ES2538997T3 (en) 2011-01-31 2015-06-25 Unilever N.V. Alkaline liquid detergent compositions
WO2012134626A2 (en) 2011-01-31 2012-10-04 Novozymes North America, Inc. Processes for enzymatic refining of pretreated cellulosic material for saccharification
CN103459586A (en) 2011-02-09 2013-12-18 诺维信公司 Cellulase enzyme mixtures for depilling and uses thereof
WO2012112718A1 (en) 2011-02-15 2012-08-23 Novozymes Biologicals, Inc. Mitigation of odor in cleaning machines and cleaning processes
WO2012110564A1 (en) 2011-02-16 2012-08-23 Novozymes A/S Detergent compositions comprising m7 or m35 metalloproteases
MX2013009176A (en) 2011-02-16 2013-08-29 Novozymes As Detergent compositions comprising metalloproteases.
WO2012110562A2 (en) 2011-02-16 2012-08-23 Novozymes A/S Detergent compositions comprising metalloproteases
CN103384678B (en) 2011-02-23 2017-01-18 诺维信股份有限公司 Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
MX2013010375A (en) 2011-03-10 2013-10-30 Unilever Nv Dye polymer.
SG192025A1 (en) 2011-03-17 2013-08-30 Danisco Us Inc Glycosyl hydrolase enzymes and uses thereof for biomass hydrolysis
RU2013146245A (en) 2011-03-17 2015-04-27 ДАНИСКО ЮЭс ИНК. METHOD FOR REDUCING VISCOSITY IN THE SUGARING PROCESS
WO2012130492A1 (en) 2011-03-25 2012-10-04 Unilever Plc Dye polymer
DK2689011T3 (en) 2011-03-25 2018-01-22 Novozymes As PROCEDURE FOR DEGRADATION OR CONVERSION OF CELLULOSE-SUBSTANCING MATERIAL
WO2012135659A2 (en) 2011-03-31 2012-10-04 Novozymes A/S Methods for enhancing the degradation or conversion of cellulosic material
ES2421162T3 (en) 2011-04-04 2013-08-29 Unilever Nv Fabric washing procedure
AR085845A1 (en) 2011-04-08 2013-10-30 Danisco Us Inc COMPOSITIONS
DE102011007313A1 (en) 2011-04-13 2012-10-18 Henkel Ag & Co. Kgaa expression methods
DE102011007627A1 (en) 2011-04-18 2012-10-18 Henkel Ag & Co. Kgaa Detergents or cleaning agents with solid enzyme preparation
DE102011007695A1 (en) 2011-04-19 2012-10-25 Henkel Ag & Co. Kgaa Phosphate-free dishwashing detergent
WO2012149192A1 (en) * 2011-04-28 2012-11-01 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
MX2013011827A (en) 2011-04-29 2014-01-08 Novozymes Inc Methods for enhancing the degradation or conversion of cellulosic material.
EP2522715A1 (en) 2011-05-13 2012-11-14 Unilever Plc, A Company Registered In England And Wales under company no. 41424 of Unilever House Aqueous concentrated laundry detergent compositions
EP2522714A1 (en) 2011-05-13 2012-11-14 Unilever Plc, A Company Registered In England And Wales under company no. 41424 of Unilever House Aqueous concentrated laundry detergent compositions
BR112013028716A2 (en) 2011-05-13 2017-01-24 Unilever Nv aqueous concentrated liquid laundry detergent, composition, method of washing polyester fabrics and their use
ES2544539T3 (en) 2011-05-26 2015-09-01 Unilever N.V. Liquid laundry composition
DE102011118032A1 (en) 2011-05-31 2012-12-06 Henkel Ag & Co. Kgaa Expression vectors for improved protein secretion
ES2671329T3 (en) 2011-06-01 2018-06-06 Unilever N.V. Liquid detergent composition containing coloring polymer
DE102011118037A1 (en) 2011-06-16 2012-12-20 Henkel Ag & Co. Kgaa Dishwashing detergent with bleach catalyst and protease
EP2537918A1 (en) 2011-06-20 2012-12-26 The Procter & Gamble Company Consumer products with lipase comprising coated particles
CN107475235B (en) 2011-06-20 2022-09-13 诺维信公司 Particulate composition
WO2012175708A2 (en) 2011-06-24 2012-12-27 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
CN112662734B (en) 2011-06-30 2024-09-10 诺维信公司 Method for screening alpha-amylase
CN107858217B (en) 2011-07-01 2021-03-23 诺维信公司 Stabilized subtilisin compositions
CN103857781A (en) 2011-07-21 2014-06-11 荷兰联合利华有限公司 Liquid laundry composition
EP2551335A1 (en) 2011-07-25 2013-01-30 The Procter & Gamble Company Enzyme stabilized liquid detergent composition
WO2013024021A1 (en) 2011-08-15 2013-02-21 Novozymes A/S Polypeptides having cellulase activity and polynucleotides encoding same
BR112014003560A2 (en) 2011-08-19 2017-03-01 Novozymes A / S polypeptide, composition, polynucleotide, nucleic acid construct or expression vector, host cell, polypeptide production methods, for enhancing the nutritional value of an animal feed, and for treating protein, plant, plant part or plant cell use of at least one polypeptide, feed additive, and feed
EP2756091A1 (en) 2011-09-13 2014-07-23 Novozymes North America, Inc. Methods of hydrolyzing and fermenting cellulosic material
WO2013041689A1 (en) 2011-09-22 2013-03-28 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
BR112014010045B1 (en) 2011-10-28 2021-12-21 Danisco Us Inc MALTO-HEXAOSE FORMING VARIANT ALPHA-AMYLASE POLYPEPTIDE, COMPOSITION COMPRISING THE SAME, HOST CELL, AND METHODS FOR REMOVING A STAIN OR AMYLACEOUS DIRT, TO SACCARIFY A COMPOSITION COMPRISING STARCH, PRODUCTION OF A FOODS COMPOSITION FOR DEFOGGING A TEXTILE
US20130109055A1 (en) 2011-10-31 2013-05-02 Bp Corporation North America Inc. Use of mammalian promoters in filamentous fungi
US9249418B2 (en) 2011-10-31 2016-02-02 Bp Corporation North America Inc. Use of plant promoters in filamentous fungi
US10351834B2 (en) 2011-11-21 2019-07-16 Novozymes, Inc. GH61 polypeptide variants and polynucleotides encoding same
US20140342433A1 (en) 2011-11-25 2014-11-20 Novozymes A/S Subtilase Variants and Polynucleotides Encoding Same
CA2856593C (en) 2011-11-25 2022-06-07 Novozymes A/S Polypeptides having lysozyme activity and polynucleotides encoding same
EP3272862A1 (en) 2011-12-16 2018-01-24 Novozymes, Inc. Polypeptides having laccase activity and polynucleotides encoding same
EP2607468A1 (en) 2011-12-20 2013-06-26 Henkel AG & Co. KGaA Detergent compositions comprising subtilase variants
US20140335596A1 (en) 2011-12-20 2014-11-13 Novozymes A/S Subtilase Variants and Polynucleotides Encoding Same
WO2013092052A1 (en) 2011-12-20 2013-06-27 Unilever Plc Isotropic liquid detergents comprising soil release polymer
EP2794866A1 (en) 2011-12-22 2014-10-29 Danisco US Inc. Compositions and methods comprising a lipolytic enzyme variant
WO2013096305A1 (en) 2011-12-22 2013-06-27 Danisco Us Inc. Variant alpha-amylases and methods of use, thereof
MX358963B (en) 2011-12-28 2018-09-11 Novozymes As Polypeptides having protease activity.
US9809787B2 (en) 2011-12-29 2017-11-07 Novozymes A/S Detergent compositions comprising lipase
AU2013213601B8 (en) 2012-01-26 2018-01-18 Novozymes A/S Use of polypeptides having protease activity in animal feed and detergents
DE102012201297A1 (en) 2012-01-31 2013-08-01 Basf Se expression methods
EP2814956B1 (en) 2012-02-17 2017-05-10 Novozymes A/S Subtilisin variants and polynucleotides encoding same
EP2628785B1 (en) 2012-02-17 2016-05-18 Henkel AG & Co. KGaA Detergent compositions comprising subtilase variants
CN104204199B (en) * 2012-02-20 2017-09-05 诺维信公司 Polypeptide and the polynucleotides for encoding the polypeptide with endoglucanase activity
US9617528B2 (en) * 2012-02-20 2017-04-11 Novozymes A/S Endoglucanase-producing recombinant host cells and methods of producing polypeptides having endoglucanase activity
EP2817325B1 (en) * 2012-02-20 2019-06-26 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
EP2639291A1 (en) 2012-03-13 2013-09-18 Unilever PLC Packaged particulate detergent composition
WO2013139702A1 (en) 2012-03-21 2013-09-26 Unilever Plc Laundry detergent particles
US20150291922A1 (en) 2012-03-29 2015-10-15 Novozymes A/S Use of Enzymes For Preparing Water Soluble Films
EP2834338B1 (en) 2012-04-03 2017-04-19 Unilever PLC, a company registered in England and Wales under company no. 41424 Laundry detergent particle
WO2013149752A1 (en) 2012-04-03 2013-10-10 Unilever Plc Laundry detergent particles
WO2013149753A1 (en) 2012-04-03 2013-10-10 Unilever Plc Laundry detergent particles
PL2834335T3 (en) 2012-04-03 2017-04-28 Unilever N.V. Laundry detergent particles
DE102012206571A1 (en) 2012-04-20 2013-10-24 Henkel Ag & Co. Kgaa Storage-stable washing or cleaning agent with increased cleaning performance
DK2841570T3 (en) 2012-04-23 2018-03-12 Novozymes As POLYPEPTIDES WITH GLUCURONYL STERASE ACTIVITY AND POLYNUCLEOTIDES CODING THEM
WO2013160025A1 (en) 2012-04-23 2013-10-31 Unilever Plc Structured aqueous liquid detergent
WO2013160248A2 (en) 2012-04-23 2013-10-31 Novozymes A/S Polypeptides having alpha-glucuronidase activity and polynucleotides encoding same
BR112014026625A2 (en) 2012-04-27 2017-07-18 Novozymes Inc E Novozymes As polypeptide variant, isolated polynucleotide, recombinant host cell, methods for producing a polypeptide variant and for obtaining a transgenic polypeptide variant, plant, plant part or cell, processes for degradation or conversion of a cellulosic material to production of a fermentation product and for the fermentation of a cellulosic material, composition, and whole broth cell culture formulation or composition
AR090971A1 (en) 2012-05-07 2014-12-17 Novozymes As POLYPEPTIDES THAT HAVE XANTANE DEGRADATION ACTIVITY AND POLYCINOCYLODES THAT CODE THEM
US8945889B2 (en) 2012-05-11 2015-02-03 Danisco Us Inc. Method of using alpha-amylase from Aspergillus clavatus for saccharification
US20150132831A1 (en) 2012-05-16 2015-05-14 Novozymes A/S Compositions Comprising Lipase and Methods of Use Thereof
US20150141316A1 (en) 2012-06-08 2015-05-21 Danisco Us Inc. Variant alpha amylases with enhanced activity on starch polymers
WO2013189802A1 (en) 2012-06-19 2013-12-27 Novozymes A/S Enzymatic reduction of hydroperoxides
US9352267B2 (en) 2012-06-20 2016-05-31 Hollingsworth & Vose Company Absorbent and/or adsorptive filter media
US20150140165A1 (en) 2012-06-20 2015-05-21 Novozymes A/S Use of polypeptides having protease activity in animal feed and detergents
US9511330B2 (en) 2012-06-20 2016-12-06 Hollingsworth & Vose Company Fibrillated fibers for liquid filtration media
US8882876B2 (en) 2012-06-20 2014-11-11 Hollingsworth & Vose Company Fiber webs including synthetic fibers
EP2885407B1 (en) * 2012-08-16 2018-06-13 Bangladesh Jute Research Institute Pectin degrading enzymes from macrophomina phaseolina and uses thereof
US20150218606A1 (en) 2012-08-16 2015-08-06 Danisco Us Inc. Method of using alpha-amylase from aspergillus clavatus and pullulanase for saccharification
TR201909592T4 (en) 2012-08-16 2019-07-22 Novozymes As The method for treating textile with endoglucanase.
CN104583394B (en) * 2012-08-16 2019-06-07 诺维信公司 Textile treating method with endoglucanases
US9328456B2 (en) 2012-08-16 2016-05-03 Novozymes A/S Method for treating textile with endoglucanase
WO2014029821A1 (en) 2012-08-22 2014-02-27 Novozymes A/S Metalloproteases from alicyclobacillus sp.
WO2014029819A1 (en) 2012-08-22 2014-02-27 Novozymes A/S Metalloprotease from exiguobacterium
US20160145540A1 (en) 2012-08-22 2016-05-26 Novozymes A/S Detergent Compositions Comprising Metalloproteases
DE102012215107A1 (en) 2012-08-24 2014-02-27 Basf Se Solid dishwashing detergent with improved protease performance
IN2015MN00417A (en) 2012-09-25 2015-09-04 Unilever Plc
CN103483049B (en) * 2012-10-30 2014-11-12 山东红日阿康化工股份有限公司 Heavy metal remover for water soluble fertilizer
US20150291656A1 (en) 2012-11-01 2015-10-15 Novozymes A/S Method for removal of dna
US20150376590A1 (en) 2012-11-02 2015-12-31 Bp Corporation North America Inc. Thermotolerant Beta-Glucosidase Variants
WO2014081622A1 (en) 2012-11-20 2014-05-30 Danisco Us Inc. Amylase with maltogenic properties
WO2014086659A2 (en) 2012-12-06 2014-06-12 Ahmedabad Textile Industry's Research Association Method for enzymatical preparation of textiles
WO2014087011A1 (en) 2012-12-07 2014-06-12 Novozymes A/S Preventing adhesion of bacteria
JP6499081B2 (en) 2012-12-11 2019-04-10 ダニスコ・ユーエス・インク Trichoderma reesei host cell expressing glucoamylase from Aspergillus fumigatus and method of use thereof
WO2014093125A1 (en) 2012-12-14 2014-06-19 Danisco Us Inc. Method of using alpha-amylase from aspergillus fumigatus and isoamylase for saccharification
US10137392B2 (en) 2012-12-14 2018-11-27 Hollingsworth & Vose Company Fiber webs coated with fiber-containing resins
WO2014090940A1 (en) 2012-12-14 2014-06-19 Novozymes A/S Removal of skin-derived body soils
DE102012224038A1 (en) 2012-12-20 2014-06-26 Henkel Ag & Co. Kgaa Enzyme-containing granular composition, used to prepare particulate washing/cleaning agents for textiles, carpets or natural fibers, comprises enzyme containing granular particles, and enzyme free granular particles with water-soluble salt
WO2014099415A1 (en) 2012-12-20 2014-06-26 Danisco Us Inc. Method of using alpha-amylase from aspergillus terreus and pullulanase for saccharification
WO2014099525A1 (en) 2012-12-21 2014-06-26 Danisco Us Inc. Paenibacillus curdlanolyticus amylase, and methods of use, thereof
US9551042B2 (en) 2012-12-21 2017-01-24 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
DK3354728T3 (en) 2012-12-21 2020-07-27 Danisco Us Inc ALPHA-amylase variants
EP2938628A4 (en) 2012-12-24 2016-10-19 Novozymes As Polypeptides having endoglucanase activity and polynucleotides encoding same
US9506050B2 (en) 2012-12-24 2016-11-29 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
US9902946B2 (en) 2013-01-03 2018-02-27 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
WO2014164777A1 (en) 2013-03-11 2014-10-09 Danisco Us Inc. Alpha-amylase combinatorial variants
CN105189724A (en) 2013-03-14 2015-12-23 诺维信公司 Enzyme and inhibitor containing water-soluble films
WO2014145768A2 (en) 2013-03-15 2014-09-18 Bp Corporation North America Inc. Use of non-fungal 5' utrs in filamentous fungi
US9631164B2 (en) 2013-03-21 2017-04-25 Novozymes A/S Polypeptides with lipase activity and polynucleotides encoding same
WO2014173980A2 (en) 2013-04-23 2014-10-30 Novozymes A/S Liquid automatic dish washing detergent compositions
JP7020778B2 (en) 2013-05-03 2022-02-16 ノボザイムス アクティーゼルスカブ Detergent enzyme microencapsulation
RU2712877C2 (en) 2013-05-14 2020-01-31 Новозимс А/С Detergent compositions
WO2014183921A1 (en) 2013-05-17 2014-11-20 Novozymes A/S Polypeptides having alpha amylase activity
CN114634921B (en) 2013-06-06 2024-09-10 诺维信公司 Alpha-amylase variants and polynucleotides encoding same
US20160130489A1 (en) 2013-06-12 2016-05-12 Novozymes A/S Dust suppressant
WO2014200656A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from streptomyces umbrinus
WO2014200657A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from streptomyces xiamenensis
WO2014200658A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from promicromonospora vindobonensis
WO2014204596A1 (en) 2013-06-17 2014-12-24 Danisco Us Inc. Alpha-amylase from bacillaceae family member
EP3013955A1 (en) 2013-06-27 2016-05-04 Novozymes A/S Subtilase variants and polynucleotides encoding same
US10378001B2 (en) 2013-06-27 2019-08-13 Novozymes A/S Subtilase variants and compositions comprising same
US20160152925A1 (en) 2013-07-04 2016-06-02 Novozymes A/S Polypeptides Having Anti-Redeposition Effect and Polynucleotides Encoding Same
CN105339492A (en) 2013-07-09 2016-02-17 诺维信公司 Polypeptides with lipase activity and polynucleotides encoding same
EP3447113B1 (en) 2013-07-12 2021-06-02 The Procter & Gamble Company Structured liquid compositions
ES2956266T3 (en) 2013-07-19 2023-12-18 Danisco Us Inc Compositions and methods comprising a lipolytic enzyme variant
RU2670946C9 (en) 2013-07-29 2018-11-26 Новозимс А/С Protease variants and polynucleotides encoding them
EP3339436B1 (en) 2013-07-29 2021-03-31 Henkel AG & Co. KGaA Detergent composition comprising protease variants
WO2015014803A1 (en) 2013-07-29 2015-02-05 Novozymes A/S Protease variants and polynucleotides encoding same
WO2015049370A1 (en) 2013-10-03 2015-04-09 Novozymes A/S Detergent composition and use of detergent composition
US20160160199A1 (en) 2013-10-03 2016-06-09 Danisco Us Inc. Alpha-amylases from exiguobacterium, and methods of use, thereof
WO2015050724A1 (en) 2013-10-03 2015-04-09 Danisco Us Inc. Alpha-amylases from a subset of exiguobacterium, and methods of use, thereof
WO2015057517A1 (en) 2013-10-17 2015-04-23 Danisco Us Inc. Use of hemicellulases to improve ethanol production
US9926548B2 (en) 2013-10-25 2018-03-27 Novozymes A/S Nucleic acid constructs and host cells for producing neurospora endoglucanases
US20160272957A1 (en) 2013-11-20 2016-09-22 Danisco Us Inc. Variant alpha-amylases having reduced susceptibility to protease cleavage, and methods of use, thereof
CN103667085B (en) * 2013-12-04 2016-01-20 青岛蔚蓝生物集团有限公司 A kind of Endoglucanase production bacterial strain
EP3083704B1 (en) 2013-12-16 2022-08-17 Nutrition & Biosciences USA 4, Inc. Use of poly alpha-1,3-glucan ethers as viscosity modifiers
EP3789407B1 (en) 2013-12-18 2024-07-24 Nutrition & Biosciences USA 4, Inc. Cationic poly alpha-1,3-glucan ethers
WO2015094809A1 (en) 2013-12-19 2015-06-25 Danisco Us Inc. Chimeric fungal alpha-amylases comprising carbohydrate binding module and the use thereof
DE102013226835A1 (en) 2013-12-20 2015-06-25 Henkel Ag & Co. Kgaa Detergents or cleaners with reduced surfactant content
US10030239B2 (en) 2013-12-20 2018-07-24 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
EP3097112B1 (en) 2014-01-22 2020-05-13 Novozymes A/S Polypeptides with lipase activity and polynucleotides encoding same
US20150232785A1 (en) 2014-02-14 2015-08-20 E I Du Pont De Nemours And Company Polysaccharides for viscosity modification
US20160348035A1 (en) 2014-03-05 2016-12-01 Novozymes A/S Compositions and Methods for Improving Properties of Non-Cellulosic Textile Materials with Xyloglucan Endotransglycosylase
US20160333292A1 (en) 2014-03-05 2016-11-17 Novozymes A/S Compositions and Methods for Improving Properties of Cellulosic Textile Materials with Xyloglucan Endotransglycosylase
US9695253B2 (en) 2014-03-11 2017-07-04 E I Du Pont De Nemours And Company Oxidized poly alpha-1,3-glucan
EP3117001B1 (en) 2014-03-12 2019-02-20 Novozymes A/S Polypeptides with lipase activity and polynucleotides encoding same
CN103865905B (en) * 2014-03-13 2015-08-19 青岛蔚蓝生物集团有限公司 A kind of endoglucanase
CN104947434B (en) * 2014-03-31 2017-07-18 河北科技大学 A kind of sulfur dyeing textile treatment composition, set group composition and processing method
EP3126479A1 (en) 2014-04-01 2017-02-08 Novozymes A/S Polypeptides having alpha amylase activity
CN112899086A (en) 2014-04-11 2021-06-04 诺维信公司 Detergent composition
US10030215B2 (en) 2014-04-15 2018-07-24 Novozymes A/S Polypeptides with lipase activity and polynucleotides encoding same
EP3760713A3 (en) 2014-05-27 2021-03-31 Novozymes A/S Lipase variants and polynucleotides encoding same
CN106459937B (en) 2014-05-27 2024-09-10 诺维信公司 Method for producing lipase
US20170121695A1 (en) 2014-06-12 2017-05-04 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
EP3158043B1 (en) 2014-06-19 2021-03-10 Nutrition & Biosciences USA 4, Inc. Compositions containing one or more poly alpha-1,3-glucan ether compounds
US9714403B2 (en) 2014-06-19 2017-07-25 E I Du Pont De Nemours And Company Compositions containing one or more poly alpha-1,3-glucan ether compounds
WO2016001319A1 (en) 2014-07-03 2016-01-07 Novozymes A/S Improved stabilization of non-protease enzyme
EP3739029A1 (en) 2014-07-04 2020-11-18 Novozymes A/S Subtilase variants and polynucleotides encoding same
EP3878960A1 (en) 2014-07-04 2021-09-15 Novozymes A/S Subtilase variants and polynucleotides encoding same
JP6268486B2 (en) 2014-08-04 2018-01-31 本田技研工業株式会社 Super thermostable endoglucanase belonging to GH family 12
EP3194543B1 (en) 2014-09-18 2018-04-04 Unilever Plc. Whitening composition
WO2016079110A2 (en) 2014-11-19 2016-05-26 Novozymes A/S Use of enzyme for cleaning
CN107075489A (en) 2014-11-20 2017-08-18 诺维信公司 Alicyclic acid bacillus variant and the polynucleotides for encoding them
CN107075493B (en) 2014-12-04 2020-09-01 诺维信公司 Subtilase variants and polynucleotides encoding same
EP4067485A3 (en) 2014-12-05 2023-01-04 Novozymes A/S Lipase variants and polynucleotides encoding same
DE102014225478A1 (en) * 2014-12-10 2016-06-16 Henkel Ag & Co. Kgaa Washing or cleaning agent with special a-amylase and defined water activity aw
DE102014225475A1 (en) 2014-12-10 2016-06-16 Henkel Ag & Co. Kgaa Washing or cleaning agent with special a-amylase and defined viscosity
EP3234121A1 (en) 2014-12-15 2017-10-25 Henkel AG & Co. KGaA Detergent composition comprising subtilase variants
CN107002049A (en) 2014-12-16 2017-08-01 诺维信公司 Polypeptide with N acerylglucosamine oxidase actives
DE102014226293A1 (en) 2014-12-17 2016-06-23 Henkel Ag & Co. Kgaa Detergent with improved stain removal
EP3234123B1 (en) 2014-12-19 2020-06-03 Novozymes A/S Protease variants and polynucleotides encoding same
WO2016097352A1 (en) 2014-12-19 2016-06-23 Novozymes A/S Protease variants and polynucleotides encoding same
DE102014226681A1 (en) 2014-12-19 2016-06-23 Henkel Ag & Co. Kgaa Liquid surfactant composition with special surfactant combination and enzyme
WO2016107567A1 (en) 2014-12-31 2016-07-07 Novozymes A/S Method of treating polyester textile
BR112017014673B1 (en) 2015-01-09 2022-11-01 Unilever Ip Holdings B.V TREATMENT COMPOSITION FOR CLOTHES WASHING
ES2702768T3 (en) 2015-02-13 2019-03-05 Unilever Nv Liquid laundry washing composition
BR112017019942A2 (en) 2015-04-02 2018-06-12 Unilever Nv liquid laundry detergent composition and polymer release for dirt release
MY186688A (en) 2015-04-08 2021-08-06 Novozymes As Process for extraction of palm oil using enzymes
AU2016245218B2 (en) 2015-04-08 2018-11-08 Novozymes A/S Process for extraction of palm oil using enzymes
CN107567489A (en) 2015-04-10 2018-01-09 诺维信公司 The purposes of laundry process, DNA enzymatic and detergent composition
CN107636134A (en) 2015-04-10 2018-01-26 诺维信公司 Detergent composition
JP6586659B2 (en) 2015-05-19 2019-10-09 本田技研工業株式会社 Thermostable glycoside hydrolase
WO2016184944A1 (en) 2015-05-19 2016-11-24 Novozymes A/S Odor reduction
EP3303411B1 (en) 2015-06-01 2020-11-18 DuPont Industrial Biosciences USA, LLC Structured liquid compositions comprising colloidal dispersions of poly alpha-1,3-glucan
US10858637B2 (en) 2015-06-16 2020-12-08 Novozymes A/S Polypeptides with lipase activity and polynucleotides encoding same
EP3310688A1 (en) 2015-06-17 2018-04-25 Novozymes A/S Container
EP3310912B1 (en) 2015-06-18 2021-01-27 Novozymes A/S Subtilase variants and polynucleotides encoding same
EP3106508B1 (en) 2015-06-18 2019-11-20 Henkel AG & Co. KGaA Detergent composition comprising subtilase variants
WO2016135351A1 (en) 2015-06-30 2016-09-01 Novozymes A/S Laundry detergent composition, method for washing and use of composition
EP3317407B1 (en) 2015-07-01 2021-05-19 Novozymes A/S Methods of reducing odor
EP3950939A3 (en) 2015-07-06 2022-06-08 Novozymes A/S Lipase variants and polynucleotides encoding same
DE102015215160A1 (en) 2015-08-07 2017-02-09 Henkel Ag & Co. Kgaa New whitening-enhancing detergent
DE102015215158A1 (en) 2015-08-07 2017-02-09 Henkel Ag & Co. Kgaa New, whiteness-enhancing detergent
DE102015215163A1 (en) 2015-08-07 2017-02-09 Henkel Ag & Co. Kgaa Detergent with ironing aid
DE102015215591A1 (en) 2015-08-14 2017-02-16 Henkel Ag & Co. Kgaa Low-water, two-phase liquid detergent with acidic pH
JP6552098B2 (en) 2015-08-20 2019-07-31 本田技研工業株式会社 Super thermostable endoglucanase
JP6244597B2 (en) 2015-08-21 2017-12-13 本田技研工業株式会社 Super thermostable endoglucanase
CN107922897A (en) 2015-08-28 2018-04-17 荷兰联合利华有限公司 The liquid detergent compositions of enzyme comprising protease and non-protein enzyme
EP3350303B1 (en) 2015-09-17 2020-04-08 Henkel AG & Co. KGaA Detergent compositions comprising polypeptides having xanthan degrading activity
CA2991114A1 (en) 2015-09-17 2017-03-23 Novozymes A/S Polypeptides having xanthan degrading activity and polynucleotides encoding same
CA2994356A1 (en) 2015-10-07 2017-04-13 Novozymes A/S Compositions comprising dnase polypeptides
US10479981B2 (en) 2015-10-14 2019-11-19 Novozymes A/S DNase variants
US10675589B2 (en) 2015-10-14 2020-06-09 Novozymes A/S Cleaning of water filtration membranes
CN108291215A (en) 2015-10-14 2018-07-17 诺维信公司 Polypeptide with proteinase activity and encode their polynucleotides
JP6997082B2 (en) 2015-10-28 2022-02-03 ノボザイムス アクティーゼルスカブ Detergent composition containing protease and amylase mutant
JP2019504932A (en) 2015-11-13 2019-02-21 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ Glucan fiber composition for use in laundry and textile care
US10844324B2 (en) 2015-11-13 2020-11-24 Dupont Industrial Biosciences Usa, Llc Glucan fiber compositions for use in laundry care and fabric care
EP3374488B1 (en) 2015-11-13 2020-10-14 DuPont Industrial Biosciences USA, LLC Glucan fiber compositions for use in laundry care and fabric care
WO2017084560A1 (en) * 2015-11-16 2017-05-26 Novozymes A/S Cellulase variants and polynucleotides encoding same
EP3380608A1 (en) 2015-11-24 2018-10-03 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
WO2017093318A1 (en) 2015-12-01 2017-06-08 Novozymes A/S Methods for producing lipases
EP3387125B1 (en) 2015-12-07 2022-10-12 Henkel AG & Co. KGaA Dishwashing compositions comprising polypeptides having beta-glucanase activity and uses thereof
CN108779448B (en) 2015-12-09 2023-08-18 丹尼斯科美国公司 Alpha-amylase combination variants
BR112018011567A2 (en) 2015-12-09 2018-11-27 Basf Se purification method of a protein of interest, and, use of a method.
US20190002819A1 (en) 2015-12-28 2019-01-03 Novozymes Bioag A/S Heat priming of bacterial spores
EP3397761B1 (en) 2015-12-30 2022-11-09 Novozymes A/S Enzyme variants and polynucleotides encoding the same
TR201808208T4 (en) 2016-01-07 2018-07-23 Unilever Nv The bitter particle.
WO2017121714A1 (en) 2016-01-15 2017-07-20 Unilever Plc Dye
MX2018008051A (en) 2016-01-29 2018-08-23 Novozymes As Beta-glucanase variants and polynucleotides encoding same.
WO2017133879A1 (en) 2016-02-04 2017-08-10 Unilever Plc Detergent liquid
EP3417039B1 (en) 2016-02-17 2019-07-10 Unilever PLC Whitening composition
CN108603140B (en) 2016-02-17 2020-09-08 荷兰联合利华有限公司 Whitening composition
WO2017162378A1 (en) 2016-03-21 2017-09-28 Unilever Plc Laundry detergent composition
BR112018069220A2 (en) 2016-03-23 2019-01-22 Novozymes As use of polypeptide that has dnase activity for tissue treatment
WO2017173324A2 (en) 2016-04-01 2017-10-05 Danisco Us Inc. Alpha-amylases, compositions & methods
WO2017173190A2 (en) 2016-04-01 2017-10-05 Danisco Us Inc. Alpha-amylases, compositions & methods
WO2017174769A2 (en) 2016-04-08 2017-10-12 Novozymes A/S Detergent compositions and uses of the same
WO2017182666A1 (en) 2016-04-22 2017-10-26 Novozymes A/S Use of phospholipase c in palm oil milling
WO2017182667A1 (en) 2016-04-22 2017-10-26 Novozymes A/S Enzyme-assisted oil extraction using sterilizer condensate
JP6959259B2 (en) 2016-04-29 2021-11-02 ノボザイムス アクティーゼルスカブ Detergent composition and its use
EP3452497B1 (en) 2016-05-03 2021-02-17 Novozymes A/S Alpha-amylase variants and polynucleotides encoding the same
CA3022121A1 (en) 2016-05-09 2017-11-16 Novozymes A/S Variant polypeptides with improved performance and use of the same
EP3464538A1 (en) 2016-05-31 2019-04-10 Novozymes A/S Stabilized liquid peroxide compositions
CN109715792A (en) 2016-06-03 2019-05-03 诺维信公司 Subtilase variants and the polynucleotides that it is encoded
WO2017211697A1 (en) 2016-06-09 2017-12-14 Unilever Plc Laundry products
WO2017220422A1 (en) 2016-06-23 2017-12-28 Novozymes A/S Use of enzymes, composition and method for removing soil
MX2018016037A (en) 2016-06-30 2019-05-30 Novozymes As Lipase variants and compositions comprising surfactant and lipase variant.
WO2018002261A1 (en) 2016-07-01 2018-01-04 Novozymes A/S Detergent compositions
CA3028535A1 (en) 2016-07-05 2018-01-11 Novozymes A/S Pectate lyase variants and polynucleotides encoding same
WO2018007573A1 (en) 2016-07-08 2018-01-11 Novozymes A/S Detergent compositions with galactanase
WO2018011276A1 (en) 2016-07-13 2018-01-18 The Procter & Gamble Company Bacillus cibi dnase variants and uses thereof
US20190292514A1 (en) 2016-07-14 2019-09-26 Basf Se Fermentation medium comprising chelating agent
US11326152B2 (en) 2016-07-18 2022-05-10 Novozymes A/S Lipase variants, polynucleotides encoding same and the use thereof
DE102016213567A1 (en) 2016-07-25 2018-01-25 Henkel Ag & Co. Kgaa Propylene glycol esters as textilpflegende ingredients
DE102016213569A1 (en) 2016-07-25 2018-01-25 Henkel Ag & Co. Kgaa Acylglutamate as textilpflegende ingredients
DE102016213568A1 (en) 2016-07-25 2018-01-25 Henkel Ag & Co. Kgaa Polymers of vinylpyrrolidone and / or vinyl acetate as textilpflegende ingredients
AU2017317563B8 (en) 2016-08-24 2023-03-23 Henkel Ag & Co. Kgaa Detergent compositions comprising xanthan lyase variants I
WO2018037065A1 (en) 2016-08-24 2018-03-01 Henkel Ag & Co. Kgaa Detergent composition comprising gh9 endoglucanase variants i
US11072765B2 (en) 2016-08-24 2021-07-27 Novozymes A/S GH9 endoglucanase variants and polynucleotides encoding same
WO2018037061A1 (en) 2016-08-24 2018-03-01 Novozymes A/S Xanthan lyase variants and polynucleotides encoding same
DE102016216014A1 (en) 2016-08-25 2018-03-01 Henkel Ag & Co. Kgaa Method for evaluating cellulases for fiber care
WO2018060475A1 (en) 2016-09-29 2018-04-05 Novozymes A/S Spore containing granule
EP3519548A1 (en) 2016-09-29 2019-08-07 Novozymes A/S Use of enzyme for washing, method for washing and warewashing composition
WO2018072979A1 (en) 2016-10-18 2018-04-26 Unilever Plc Whitening composition
WO2018077938A1 (en) 2016-10-25 2018-05-03 Novozymes A/S Detergent compositions
EP3535377B1 (en) 2016-11-01 2022-02-09 Novozymes A/S Multi-core granules
DE102016221849A1 (en) 2016-11-08 2018-05-09 Henkel Ag & Co. Kgaa A surfactant composition containing an amylase
KR20190086540A (en) 2016-12-01 2019-07-22 바스프 에스이 Stabilization of enzymes in the composition
WO2018108865A1 (en) 2016-12-12 2018-06-21 Novozymes A/S Use of polypeptides
BR112019011999B1 (en) 2016-12-15 2022-11-08 Unilever Ip Holdings B.V COMPOSITION OF AQUEOUS LIQUID DETERGENT FOR WASHING CLOTHES AND DOMESTIC METHOD OF TREATMENT OF A FABRIC
US10385291B2 (en) 2016-12-22 2019-08-20 Henkel Ag & Co. Kgaa Liquid surfactant compositions and associated methods
US10047321B2 (en) 2016-12-22 2018-08-14 Henkel Ag & Co. Kgaa Liquid surfactant compositions having a modified oxo-alcohol derivative
EP3580316B1 (en) 2017-02-13 2023-11-01 Unilever IP Holdings B.V. Garment laundering system
BR112019019987A2 (en) 2017-03-24 2020-04-28 Unilever Nv detergent composition and white fabric washing method
DK3378936T3 (en) 2017-03-24 2021-08-16 Clariant Int Ltd Cellulase suitable for use in detergent compositions
CN110662836B (en) 2017-03-31 2024-04-12 丹尼斯科美国公司 Alpha-amylase combination variants
WO2018177938A1 (en) 2017-03-31 2018-10-04 Novozymes A/S Polypeptides having dnase activity
EP3601549A1 (en) 2017-03-31 2020-02-05 Novozymes A/S Polypeptides having dnase activity
US11149233B2 (en) 2017-03-31 2021-10-19 Novozymes A/S Polypeptides having RNase activity
CN114480034A (en) 2017-04-04 2022-05-13 诺维信公司 Glycosyl hydrolase
WO2018185150A1 (en) 2017-04-04 2018-10-11 Novozymes A/S Polypeptides
EP3607040A1 (en) 2017-04-04 2020-02-12 Novozymes A/S Polypeptide compositions and uses thereof
ES2728758T3 (en) 2017-04-05 2019-10-28 Henkel Ag & Co Kgaa Detergent compositions comprising bacterial mannanas
EP3385362A1 (en) 2017-04-05 2018-10-10 Henkel AG & Co. KGaA Detergent compositions comprising fungal mannanases
US20200190437A1 (en) 2017-04-06 2020-06-18 Novozymes A/S Cleaning compositions and uses thereof
EP3607042A1 (en) 2017-04-06 2020-02-12 Novozymes A/S Cleaning compositions and uses thereof
EP3626809A1 (en) 2017-04-06 2020-03-25 Novozymes A/S Cleaning compositions and uses thereof
WO2018184816A1 (en) 2017-04-06 2018-10-11 Novozymes A/S Cleaning compositions and uses thereof
CA3058520A1 (en) 2017-04-06 2018-10-11 Novozymes A/S Detergent compositions and uses thereof
MX2019011764A (en) 2017-04-06 2019-11-28 Novozymes As Cleaning compositions and uses thereof.
EP3607043A1 (en) 2017-04-06 2020-02-12 Novozymes A/S Cleaning compositions and uses thereof
WO2018184818A1 (en) 2017-04-06 2018-10-11 Novozymes A/S Cleaning compositions and uses thereof
EP3619304A1 (en) 2017-05-05 2020-03-11 Novozymes A/S Compositions comprising lipase and sulfite
EP3401385A1 (en) 2017-05-08 2018-11-14 Henkel AG & Co. KGaA Detergent composition comprising polypeptide comprising carbohydrate-binding domain
WO2018206535A1 (en) 2017-05-08 2018-11-15 Novozymes A/S Carbohydrate-binding domain and polynucleotides encoding the same
CN110741084B (en) 2017-05-08 2024-04-12 诺维信公司 Mannanase variants and polynucleotides encoding same
EP3622064A1 (en) 2017-05-08 2020-03-18 Novozymes A/S Mannanase variants and polynucleotides encoding same
EP3412761A1 (en) 2017-06-07 2018-12-12 Henkel AG & Co. KGaA Anti-pilling laundry sheet
WO2018234056A1 (en) 2017-06-20 2018-12-27 Unilever N.V. Particulate detergent composition comprising perfume
WO2018234003A1 (en) 2017-06-21 2018-12-27 Unilever Plc Packaging and dispensing of detergent compositions
CN111108183A (en) 2017-06-30 2020-05-05 诺维信公司 Enzyme slurry composition
EP3649221B8 (en) 2017-07-07 2024-05-29 Unilever IP Holdings B.V. Laundry cleaning composition
WO2019008036A1 (en) 2017-07-07 2019-01-10 Unilever Plc Whitening composition
DE102017115765A1 (en) 2017-07-13 2019-01-17 Miele & Cie. Kg Process for treating textiles and washing machine
CN111032856A (en) 2017-08-07 2020-04-17 诺维信公司 Use of FCA control based on pH
CN111212906B (en) 2017-08-18 2024-02-02 丹尼斯科美国公司 Alpha-amylase variants
CN111278971A (en) 2017-08-24 2020-06-12 诺维信公司 GH9 endoglucanase variants and polynucleotides encoding same
EP3673057A1 (en) 2017-08-24 2020-07-01 Novozymes A/S Xanthan lyase variants and polynucleotides encoding same
US11624059B2 (en) 2017-08-24 2023-04-11 Henkel Ag & Co. Kgaa Detergent compositions comprising GH9 endoglucanase variants II
WO2019038187A1 (en) 2017-08-24 2019-02-28 Unilever Plc Improvements relating to fabric cleaning
WO2019038060A1 (en) 2017-08-24 2019-02-28 Henkel Ag & Co. Kgaa Detergent composition comprising xanthan lyase variants ii
WO2019038186A1 (en) 2017-08-24 2019-02-28 Unilever Plc Improvements relating to fabric cleaning
DE102017215015A1 (en) 2017-08-28 2019-02-28 Henkel Ag & Co. Kgaa Method for improved expression of enzymes
EP3684897A1 (en) 2017-09-20 2020-07-29 Novozymes A/S Use of enzymes for improving water absorption and/or whiteness
US11414814B2 (en) 2017-09-22 2022-08-16 Novozymes A/S Polypeptides
EP3461892A3 (en) 2017-09-27 2019-06-12 The Procter & Gamble Company Detergent compositions comprising lipases
US11332725B2 (en) 2017-09-27 2022-05-17 Novozymes A/S Lipase variants and microcapsule compositions comprising such lipase variants
US11746310B2 (en) 2017-10-02 2023-09-05 Novozymes A/S Polypeptides having mannanase activity and polynucleotides encoding same
CN111373036A (en) 2017-10-02 2020-07-03 诺维信公司 Polypeptides having mannanase activity and polynucleotides encoding same
WO2019076800A1 (en) 2017-10-16 2019-04-25 Novozymes A/S Cleaning compositions and uses thereof
EP3697881A1 (en) 2017-10-16 2020-08-26 Novozymes A/S Low dusting granules
CN111542589A (en) 2017-10-16 2020-08-14 诺维信公司 Low dusting particles
WO2019081515A1 (en) 2017-10-24 2019-05-02 Novozymes A/S Compositions comprising polypeptides having mannanase activity
EP3701017A1 (en) 2017-10-27 2020-09-02 Novozymes A/S Dnase variants
EP3476936B1 (en) 2017-10-27 2022-02-09 The Procter & Gamble Company Detergent compositions comprising polypeptide variants
DE102017125559A1 (en) 2017-11-01 2019-05-02 Henkel Ag & Co. Kgaa CLEANSING COMPOSITIONS CONTAINING DISPERSINE II
EP3704219B1 (en) 2017-11-01 2024-01-10 Novozymes A/S Polypeptides and compositions comprising such polypeptides
DE102017125560A1 (en) 2017-11-01 2019-05-02 Henkel Ag & Co. Kgaa CLEANSING COMPOSITIONS CONTAINING DISPERSINE III
WO2019086532A1 (en) 2017-11-01 2019-05-09 Novozymes A/S Methods for cleaning medical devices
DE102017125558A1 (en) 2017-11-01 2019-05-02 Henkel Ag & Co. Kgaa CLEANING COMPOSITIONS CONTAINING DISPERSINE I
EP3704240A1 (en) 2017-11-01 2020-09-09 Novozymes A/S Polypeptides and compositions comprising such polypeptides
BR112020009093A2 (en) 2017-11-09 2020-10-13 Basf Se enzyme particle, washing or cleaning and food or feed compositions, and use of an organic white pigment
BR112020010648A2 (en) 2017-11-30 2021-02-02 Unilever N.V. detergent composition, laundry detergent composition, method to improve enzymatic cleaning in water and use of a protease enzyme
EP3720954A1 (en) 2017-12-04 2020-10-14 Novozymes A/S Lipase variants and polynucleotides encoding same
CN109988714B (en) * 2017-12-29 2021-12-28 青岛蔚蓝生物集团有限公司 Trichoderma reesei and application thereof
WO2019154954A1 (en) 2018-02-08 2019-08-15 Novozymes A/S Lipase variants and compositions thereof
WO2019154951A1 (en) 2018-02-08 2019-08-15 Novozymes A/S Lipases, lipase variants and compositions thereof
KR20200124258A (en) 2018-02-23 2020-11-02 헨켈 아게 운트 코. 카게아아 Detergent composition comprising xanthan lyase and endoglucanase variant
CN111770788B (en) 2018-03-13 2023-07-25 诺维信公司 Microencapsulation using amino sugar oligomers
WO2019180111A1 (en) 2018-03-23 2019-09-26 Novozymes A/S Subtilase variants and compositions comprising same
US11535837B2 (en) 2018-03-29 2022-12-27 Novozymes A/S Mannanase variants and polynucleotides encoding same
WO2019201793A1 (en) 2018-04-17 2019-10-24 Novozymes A/S Polypeptides comprising carbohydrate binding activity in detergent compositions and their use in reducing wrinkles in textile or fabric.
WO2019201785A1 (en) 2018-04-19 2019-10-24 Novozymes A/S Stabilized cellulase variants
US11661592B2 (en) 2018-04-19 2023-05-30 Novozymes A/S Stabilized endoglucanase variants
EP3775122A1 (en) 2018-05-17 2021-02-17 Unilever PLC Cleaning composition comprising rhamnolipid and alkyl ether carboxylate surfactants
WO2019219531A1 (en) 2018-05-17 2019-11-21 Unilever Plc Cleaning composition
WO2019241614A1 (en) 2018-06-14 2019-12-19 Ecolab Usa Inc. Compositions comprising enzyme and quaternary ammonium compounds
WO2019241615A1 (en) 2018-06-14 2019-12-19 Ecolab Usa Inc. Synergistic cellulase-surfactant interactions for degradation of bacterial cellulose
CN112368363A (en) 2018-06-28 2021-02-12 诺维信公司 Detergent composition and use thereof
EP3814489A1 (en) 2018-06-29 2021-05-05 Novozymes A/S Subtilase variants and compositions comprising same
EP3814473A1 (en) 2018-06-29 2021-05-05 Novozymes A/S Detergent compositions and uses thereof
CN112352039B (en) 2018-07-02 2022-11-15 诺维信公司 Cleaning composition and use thereof
EP3818138A1 (en) 2018-07-03 2021-05-12 Novozymes A/S Cleaning compositions and uses thereof
WO2020008024A1 (en) 2018-07-06 2020-01-09 Novozymes A/S Cleaning compositions and uses thereof
WO2020008043A1 (en) 2018-07-06 2020-01-09 Novozymes A/S Cleaning compositions and uses thereof
BR112021000294A2 (en) * 2018-07-10 2021-04-06 Novozymes A/S METHODS OF PRODUCTION OF PAPER OR CARDBOARD, IMPROVING AT LEAST ONE PROPERTY OF A SEMI-FLUID PULP FOLDER FOR USE IN A PAPER OR CARDBOARD MILL, OF PRODUCTION OF A PULP OR SEMI-FLUID PULP, PAPER OR CARDBOARD PRODUCTION OR FOR PRODUCTION OF A PULP OR SEMI-FLUID PULP, PULP OR SEMI-FLUID PULP OF TREATED PULP, PAPER OR CARD PRODUCT PRODUCED USING THE SEMI-FLUID PULP OF TREATED PULP, AND, MIXTURE
BR112021000774A2 (en) 2018-07-17 2021-04-13 Unilever Ip Holdings B.V. USE OF RAMNOLIPID IN A SURFACE SYSTEM FOR MANUAL WASHING DETERGENTS
CN112543801A (en) 2018-07-27 2021-03-23 荷兰联合利华有限公司 Laundry detergent
WO2020028443A1 (en) 2018-07-31 2020-02-06 Danisco Us Inc Variant alpha-amylases having amino acid substitutions that lower the pka of the general acid
BR112021004507A2 (en) 2018-09-17 2021-06-08 Unilever Ip Holdings B.V. detergent composition, method of treating a substrate with a detergent composition and use of a bacterial lipase enzyme
US20210340466A1 (en) 2018-10-01 2021-11-04 Novozymes A/S Detergent compositions and uses thereof
WO2020070014A1 (en) 2018-10-02 2020-04-09 Novozymes A/S Cleaning composition comprising anionic surfactant and a polypeptide having rnase activity
WO2020070011A1 (en) 2018-10-02 2020-04-09 Novozymes A/S Cleaning composition
WO2020070209A1 (en) 2018-10-02 2020-04-09 Novozymes A/S Cleaning composition
WO2020070249A1 (en) 2018-10-03 2020-04-09 Novozymes A/S Cleaning compositions
WO2020070199A1 (en) 2018-10-03 2020-04-09 Novozymes A/S Polypeptides having alpha-mannan degrading activity and polynucleotides encoding same
WO2020074499A1 (en) 2018-10-09 2020-04-16 Novozymes A/S Cleaning compositions and uses thereof
WO2020074498A1 (en) 2018-10-09 2020-04-16 Novozymes A/S Cleaning compositions and uses thereof
DE102018217397A1 (en) 2018-10-11 2020-04-16 Henkel Ag & Co. Kgaa Use of transition metal-free tinting dyes in combination with catechol derivatives
DE102018217392A1 (en) 2018-10-11 2020-04-16 Henkel Ag & Co. Kgaa Multi-component detergent with catechol metal complex
DE102018217398A1 (en) 2018-10-11 2020-04-16 Henkel Ag & Co. Kgaa Liquid detergent with dihydroxy terephthalic acid diamide compound
DE102018217399A1 (en) 2018-10-11 2020-04-16 Henkel Ag & Co. Kgaa Liquid composition with dihydroxy terephthalic acid diamide compound and high amount of surfactant
WO2020074545A1 (en) 2018-10-11 2020-04-16 Novozymes A/S Cleaning compositions and uses thereof
DE102018217393A1 (en) 2018-10-11 2020-04-16 Henkel Ag & Co. Kgaa Detergent composition with catechol metal complex compound
WO2020077331A2 (en) 2018-10-12 2020-04-16 Danisco Us Inc Alpha-amylases with mutations that improve stability in the presence of chelants
EP3647397A1 (en) 2018-10-31 2020-05-06 Henkel AG & Co. KGaA Cleaning compositions containing dispersins iv
EP3647398B1 (en) 2018-10-31 2024-05-15 Henkel AG & Co. KGaA Cleaning compositions containing dispersins v
CN113015781B (en) 2018-11-20 2022-09-13 联合利华知识产权控股有限公司 Detergent composition
CN113166689A (en) 2018-11-20 2021-07-23 联合利华知识产权控股有限公司 Detergent composition
CN113056549B (en) 2018-11-20 2023-03-10 联合利华知识产权控股有限公司 Detergent composition
BR112021009828A2 (en) 2018-11-20 2021-08-17 Unilever Ip Holdings B.V. liquid detergent composition, method of treating a fabric substrate and use of a sterol esterase enzyme
BR112021009789A2 (en) 2018-11-20 2021-08-17 Unilever Ip Holdings B.V. detergent composition, method of treating a fabric substrate and use of an esterase enzyme
EP3891264A1 (en) 2018-12-03 2021-10-13 Novozymes A/S LOW pH POWDER DETERGENT COMPOSITION
US20220056379A1 (en) 2018-12-03 2022-02-24 Novozymes A/S Powder Detergent Compositions
EP3666872B1 (en) 2018-12-12 2021-08-11 Henkel AG & Co. KGaA Phosphonated acrylic copolymers for surface hydrophilization
EP3898962A2 (en) 2018-12-21 2021-10-27 Novozymes A/S Polypeptides having peptidoglycan degrading activity and polynucleotides encoding same
WO2020127775A1 (en) 2018-12-21 2020-06-25 Novozymes A/S Detergent pouch comprising metalloproteases
WO2020150386A1 (en) 2019-01-16 2020-07-23 Fornia Biosolutions, Inc. Endoglucanase compositions and methods
BR112021014332A2 (en) 2019-01-22 2021-09-28 Unilever Ip Holdings B.V. LAUNDRY DETERGENT COMPOSITION, DOMESTIC METHOD TO TREAT A FABRIC AND NON-LEUCO BLUE OR VIOLET TONER DYE
US20220098520A1 (en) 2019-01-22 2022-03-31 Conopco, Inc., D/B/A Unilever Laundry detergent
EP3702452A1 (en) 2019-03-01 2020-09-02 Novozymes A/S Detergent compositions comprising two proteases
BR112021018731A2 (en) 2019-03-21 2021-12-21 Novozymes As Alpha-amylase variants and polynucleotides encoding them
EP3715445A1 (en) 2019-03-29 2020-09-30 Clariant Produkte (Deutschland) GmbH Cellulolytic enzyme comprising laundry product
CN113785039B (en) 2019-04-03 2024-06-18 诺维信公司 Polypeptides having beta-glucanase activity, polynucleotides encoding same and use thereof in cleaning and detergent compositions
DE102019204792A1 (en) 2019-04-04 2020-10-08 Henkel Ag & Co. Kgaa Use of mannanase enzyme in combination with catechol derivatives
WO2020207944A1 (en) 2019-04-10 2020-10-15 Novozymes A/S Polypeptide variants
US20220186151A1 (en) 2019-04-12 2022-06-16 Novozymes A/S Stabilized glycoside hydrolase variants
WO2020229535A1 (en) 2019-05-16 2020-11-19 Unilever Plc Laundry composition
EP3990603B1 (en) 2019-06-28 2022-12-07 Unilever Global Ip Limited Detergent composition
WO2020260006A1 (en) 2019-06-28 2020-12-30 Unilever Plc Detergent compositions
CN113891930A (en) 2019-06-28 2022-01-04 联合利华知识产权控股有限公司 Detergent composition
CN113906124B (en) 2019-06-28 2024-08-02 联合利华知识产权控股有限公司 Detergent composition
EP3990604B1 (en) 2019-06-28 2022-12-14 Unilever Global IP Limited Detergent composition
US20220372397A1 (en) 2019-06-28 2022-11-24 Conopco, Inc., D/B/A Unilever Detergent composition
MX2021015894A (en) 2019-07-01 2022-02-03 Basf Se Peptide acetals for stabilising enzymes.
EP3994255A1 (en) 2019-07-02 2022-05-11 Novozymes A/S Lipase variants and compositions thereof
CN114364778B (en) 2019-07-12 2024-08-13 诺维信公司 Enzymatic emulsions for detergents
US20240294852A1 (en) 2019-08-27 2024-09-05 Novozymes A/S Composition comprising a lipase
WO2021037895A1 (en) 2019-08-27 2021-03-04 Novozymes A/S Detergent composition
BR112022003050A2 (en) 2019-09-02 2022-05-17 Unilever Ip Holdings B V Aqueous laundry detergent composition and household method for treating a fabric
WO2021053127A1 (en) 2019-09-19 2021-03-25 Novozymes A/S Detergent composition
CN114423851A (en) 2019-09-19 2022-04-29 联合利华知识产权控股有限公司 Detergent composition
EP4038170A1 (en) 2019-10-03 2022-08-10 Novozymes A/S Polypeptides comprising at least two carbohydrate binding domains
AR120142A1 (en) 2019-10-07 2022-02-02 Unilever Nv DETERGENT COMPOSITION
US20220403359A1 (en) 2019-10-24 2022-12-22 Danisco Us Inc Variant maltopentaose/maltohexaose-forming alpha-amylases
BR112021021050A2 (en) 2019-11-29 2022-09-13 Basf Se COMPOSITION, USE OF A COMPOSITION, POLYMER, PROCESS FOR PREPARING POLYMERS, AND, METHOD FOR IMPROVING CLEANING PERFORMANCE OF A LIQUID DETERGENT COMPOSITION
EP4077619A1 (en) 2019-12-20 2022-10-26 Henkel AG & Co. KGaA Cleaning composition coprising a dispersin and a carbohydrase
CN114846128A (en) 2019-12-20 2022-08-02 汉高股份有限及两合公司 Cleaning compositions comprising disperse protein VIII
US20220411773A1 (en) 2019-12-20 2022-12-29 Novozymes A/S Polypeptides having proteolytic activity and use thereof
EP4077617A1 (en) 2019-12-20 2022-10-26 Novozymes A/S Stabilized liquid boron-free enzyme compositions
US20230048546A1 (en) 2019-12-20 2023-02-16 Henkel Ag & Co. Kgaa Cleaning compositions comprising dispersins vi
CN114761527A (en) 2019-12-23 2022-07-15 宝洁公司 Compositions comprising enzymes
WO2021130167A1 (en) 2019-12-23 2021-07-01 Novozymes A/S Enzyme compositions and uses thereof
US20230159861A1 (en) 2020-01-23 2023-05-25 Novozymes A/S Enzyme compositions and uses thereof
EP4097226A1 (en) 2020-01-31 2022-12-07 Novozymes A/S Mannanase variants and polynucleotides encoding same
CN115052981A (en) 2020-01-31 2022-09-13 诺维信公司 Mannanase variants and polynucleotides encoding same
EP3892708A1 (en) 2020-04-06 2021-10-13 Henkel AG & Co. KGaA Cleaning compositions comprising dispersin variants
MX2022011948A (en) 2020-04-08 2022-10-21 Novozymes As Carbohydrate binding module variants.
WO2021214059A1 (en) 2020-04-21 2021-10-28 Novozymes A/S Cleaning compositions comprising polypeptides having fructan degrading activity
EP3907271A1 (en) 2020-05-07 2021-11-10 Novozymes A/S Cleaning composition, use and method of cleaning
BR112022024537A2 (en) 2020-06-08 2022-12-27 Unilever Ip Holdings B V METHOD OF ENHANCEMENT OF PROTEASE ACTIVITY IN A DETERGENT COMPOSITION AND USE OF SAPONIN
WO2021259099A1 (en) 2020-06-24 2021-12-30 Novozymes A/S Use of cellulases for removing dust mite from textile
EP3936593A1 (en) 2020-07-08 2022-01-12 Henkel AG & Co. KGaA Cleaning compositions and uses thereof
CN111560363B (en) * 2020-07-16 2021-04-23 凯莱英生命科学技术(天津)有限公司 PVA film immobilized enzyme and preparation method thereof
WO2022023250A1 (en) 2020-07-27 2022-02-03 Unilever Ip Holdings B.V. Use of an enzyme and surfactant for inhibiting microorganisms
CN116323889A (en) 2020-08-25 2023-06-23 诺维信公司 Family 44 xyloglucanase variants
WO2022043042A1 (en) 2020-08-28 2022-03-03 Unilever Ip Holdings B.V. Detergent composition
CN116018396A (en) 2020-08-28 2023-04-25 联合利华知识产权控股有限公司 Detergent composition
EP4204526B1 (en) 2020-08-28 2024-04-24 Unilever IP Holdings B.V. Surfactant and detergent composition
WO2022042977A1 (en) 2020-08-28 2022-03-03 Unilever Ip Holdings B.V. Detergent composition
US20230287300A1 (en) 2020-08-28 2023-09-14 Conopco, Inc., D/B/A Unilever Surfactant and detergent composition
US20240240114A1 (en) 2020-09-22 2024-07-18 Basf Se Improved Combination of Protease and Protease Inhibitor with Secondary Enzyme
EP4225905A2 (en) 2020-10-07 2023-08-16 Novozymes A/S Alpha-amylase variants
EP4232539A2 (en) 2020-10-20 2023-08-30 Novozymes A/S Use of polypeptides having dnase activity
WO2022090361A2 (en) 2020-10-29 2022-05-05 Novozymes A/S Lipase variants and compositions comprising such lipase variants
WO2022103725A1 (en) 2020-11-13 2022-05-19 Novozymes A/S Detergent composition comprising a lipase
EP4256020A1 (en) 2020-12-07 2023-10-11 Unilever IP Holdings B.V. Detergent compositions
US20240010951A1 (en) 2020-12-07 2024-01-11 Conopco Inc., D/B/A Unilever Detergent compositions
US20240002751A1 (en) 2020-12-17 2024-01-04 Conopco, Inc., D/B/A Unilever Cleaning composition
CN116583583A (en) 2020-12-17 2023-08-11 联合利华知识产权控股有限公司 Use and cleaning composition
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
US11987824B2 (en) 2020-12-22 2024-05-21 Fornia Biosolutions, Inc. Additional endoglucanase variants and methods
DE102021100563A1 (en) 2021-01-13 2022-07-14 Henkel Ag & Co. Kgaa COMPOSITION CONTAINING PERFUMES AND ENZYMES
EP4032966A1 (en) 2021-01-22 2022-07-27 Novozymes A/S Liquid enzyme composition with sulfite scavenger
EP4039806A1 (en) 2021-02-04 2022-08-10 Henkel AG & Co. KGaA Detergent composition comprising xanthan lyase and endoglucanase variants with im-proved stability
EP4291646A2 (en) 2021-02-12 2023-12-20 Novozymes A/S Alpha-amylase variants
EP4291625A1 (en) 2021-02-12 2023-12-20 Novozymes A/S Stabilized biological detergents
EP4053256A1 (en) 2021-03-01 2022-09-07 Novozymes A/S Use of enzymes for improving fragrance deposition
WO2022189521A1 (en) 2021-03-12 2022-09-15 Novozymes A/S Polypeptide variants
US20240060061A1 (en) 2021-03-15 2024-02-22 Novozymes A/S Dnase variants
EP4060036A1 (en) 2021-03-15 2022-09-21 Novozymes A/S Polypeptide variants
WO2022199418A1 (en) 2021-03-26 2022-09-29 Novozymes A/S Detergent composition with reduced polymer content
EP4347772A1 (en) 2021-05-25 2024-04-10 Unilever IP Holdings B.V. Laundry method
WO2022268885A1 (en) 2021-06-23 2022-12-29 Novozymes A/S Alpha-amylase polypeptides
KR20240027619A (en) 2021-06-30 2024-03-04 헨켈 아게 운트 코. 카게아아 Compositions with improved moisture management performance
CN117580939A (en) 2021-06-30 2024-02-20 汉高股份有限及两合公司 Cleaning compositions having improved anti-dusting and/or anti-pilling properties
CN113785844A (en) * 2021-08-11 2021-12-14 中山市天图精细化工有限公司 Aerosol for toilet disinfection foam and preparation method thereof
WO2023041694A1 (en) 2021-09-20 2023-03-23 Unilever Ip Holdings B.V. Detergent composition
CA3241094A1 (en) 2021-12-16 2023-06-22 Jonathan LASSILA Variant maltopentaose/maltohexaose-forming alpha-amylases
WO2023117895A1 (en) 2021-12-21 2023-06-29 Basf Se Chemical product passport for production data
WO2023116569A1 (en) 2021-12-21 2023-06-29 Novozymes A/S Composition comprising a lipase and a booster
CN114032228B (en) * 2021-12-24 2024-03-08 武汉新华扬生物股份有限公司 Acid cellulase Cel-Bi and gene and application thereof
EP4206309A1 (en) 2021-12-30 2023-07-05 Novozymes A/S Protein particles with improved whiteness
CN118591614A (en) 2022-01-28 2024-09-03 联合利华知识产权控股有限公司 Laundry compositions
WO2023144071A1 (en) 2022-01-28 2023-08-03 Unilever Ip Holdings B.V. Laundry composition
EP4234664A1 (en) 2022-02-24 2023-08-30 Evonik Operations GmbH Composition comprising glucolipids and enzymes
CN118660951A (en) 2022-03-02 2024-09-17 诺维信公司 Use of xyloglucanase for improving the sustainability of detergents
AU2023228020A1 (en) 2022-03-04 2024-07-11 Novozymes A/S Dnase variants and compositions
AU2023250091A1 (en) 2022-04-08 2024-10-03 Novozymes A/S Hexosaminidase variants and compositions
CN114958898B (en) * 2022-05-20 2023-11-14 中国农业科学院郑州果树研究所 Establishment method and application of PEG-mediated genetic transformation system of aschersonia phaseoli
WO2023227375A1 (en) 2022-05-27 2023-11-30 Unilever Ip Holdings B.V. Laundry liquid composition comprising a surfactant, an aminocarboxylate, an organic acid and a fragrance
WO2023227331A1 (en) 2022-05-27 2023-11-30 Unilever Ip Holdings B.V. Composition comprising a specific methyl ester ethoxylate surfactant and a lipase
WO2023227335A1 (en) 2022-05-27 2023-11-30 Unilever Ip Holdings B.V. Liquid composition comprising linear alkyl benzene sulphonate, methyl ester ethoxylate and alkoxylated zwitterionic polyamine polymer
WO2023227421A1 (en) 2022-05-27 2023-11-30 Unilever Ip Holdings B.V. Laundry liquid composition comprising a surfactant, an alkoxylated zwitterionic polyamine polymer, and a fragrance
WO2023227356A1 (en) 2022-05-27 2023-11-30 Unilever Ip Holdings B.V. Composition containing enzyme
WO2023227332A1 (en) 2022-05-27 2023-11-30 Unilever Ip Holdings B.V. Laundry liquid composition comprising a surfactant, an alkoxylated zwitterionic polyamine polymer and a protease
DE102022205593A1 (en) 2022-06-01 2023-12-07 Henkel Ag & Co. Kgaa DETERGENT AND CLEANING AGENTS WITH IMPROVED ENZYME STABILITY
DE102022205591A1 (en) 2022-06-01 2023-12-07 Henkel Ag & Co. Kgaa DETERGENT AND CLEANING AGENTS WITH IMPROVED ENZYME STABILITY
DE102022205588A1 (en) 2022-06-01 2023-12-07 Henkel Ag & Co. Kgaa DETERGENT AND CLEANING AGENTS WITH IMPROVED ENZYME STABILITY
DE102022205594A1 (en) 2022-06-01 2023-12-07 Henkel Ag & Co. Kgaa PERFORMANCE-IMPROVED AND STORAGE-STABLE PROTEASE VARIANTS
WO2023247348A1 (en) 2022-06-21 2023-12-28 Novozymes A/S Mannanase variants and polynucleotides encoding same
WO2023247664A2 (en) 2022-06-24 2023-12-28 Novozymes A/S Lipase variants and compositions comprising such lipase variants
WO2024028160A1 (en) 2022-08-04 2024-02-08 Unilever Ip Holdings B.V. Packaged homecare product
WO2024028161A1 (en) 2022-08-04 2024-02-08 Unilever Ip Holdings B.V. Packaged homecare product
WO2024028159A1 (en) 2022-08-04 2024-02-08 Unilever Ip Holdings B.V. Packaged homecare product
WO2024033135A2 (en) 2022-08-11 2024-02-15 Basf Se Amylase variants
WO2024033136A1 (en) 2022-08-11 2024-02-15 Basf Se Amylase variants
WO2024037686A1 (en) 2022-08-16 2024-02-22 Henkel Ag & Co. Kgaa Performance-enhanced protease variants x
WO2024037685A1 (en) 2022-08-16 2024-02-22 Henkel Ag & Co. Kgaa Performance-enhanced protease variants ix
DE102022208891A1 (en) 2022-08-16 2024-02-22 Henkel Ag & Co. Kgaa PERFORMANCE IMPROVED PROTEASE VARIANTS
DE102022208890A1 (en) 2022-08-16 2024-02-22 Henkel Ag & Co. Kgaa PERFORMANCE IMPROVED PROTEASE VARIANTS IX
EP4324900A1 (en) 2022-08-17 2024-02-21 Henkel AG & Co. KGaA Detergent composition comprising enzymes
WO2024046952A1 (en) 2022-08-30 2024-03-07 Novozymes A/S Improvements in or relating to organic compounds
DE102022209245A1 (en) 2022-09-06 2024-03-07 Henkel Ag & Co. Kgaa DETERGENT AND CLEANING PRODUCT CONTAINING TANNASE I
DE102022209246A1 (en) 2022-09-06 2024-03-07 Henkel Ag & Co. Kgaa DETERGENT AND CLEANING PRODUCT CONTAINING TANNASE II
WO2024056332A1 (en) 2022-09-13 2024-03-21 Unilever Ip Holdings B.V. Washing machine and washing method
WO2024056334A1 (en) 2022-09-13 2024-03-21 Unilever Ip Holdings B.V. Washing machine and washing method
WO2024056333A1 (en) 2022-09-13 2024-03-21 Unilever Ip Holdings B.V. Washing machine and washing method
WO2024056278A1 (en) 2022-09-13 2024-03-21 Unilever Ip Holdings B.V. Washing machine and washing method
EP4349947A1 (en) 2022-10-05 2024-04-10 Unilever IP Holdings B.V. Laundry liquid composition
EP4349943A1 (en) 2022-10-05 2024-04-10 Unilever IP Holdings B.V. Laundry liquid composition
EP4349945A1 (en) 2022-10-05 2024-04-10 Unilever IP Holdings B.V. Laundry liquid composition
EP4349946A1 (en) 2022-10-05 2024-04-10 Unilever IP Holdings B.V. Unit dose fabric treatment product
EP4349944A1 (en) 2022-10-05 2024-04-10 Unilever IP Holdings B.V. Laundry liquid composition
EP4349942A1 (en) 2022-10-05 2024-04-10 Unilever IP Holdings B.V. Laundry liquid composition
EP4349948A1 (en) 2022-10-05 2024-04-10 Unilever IP Holdings B.V. Laundry liquid composition
WO2024088706A1 (en) 2022-10-25 2024-05-02 Unilever Ip Holdings B.V. Composition
WO2024088716A1 (en) 2022-10-25 2024-05-02 Unilever Ip Holdings B.V. Composition
EP4361239A1 (en) 2022-10-25 2024-05-01 Unilever IP Holdings B.V. Laundry liquid composition
DE102022211482A1 (en) 2022-10-28 2024-05-08 Henkel Ag & Co. Kgaa Natural dye coloring with improved color intensity
WO2024094735A1 (en) 2022-11-04 2024-05-10 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2024094732A1 (en) 2022-11-04 2024-05-10 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2024094733A1 (en) 2022-11-04 2024-05-10 Basf Se Polypeptides having protease activity for use in detergent compositions
DE102022211856A1 (en) 2022-11-09 2024-05-16 Henkel Ag & Co. Kgaa Two-stage dyeing process with natural dyes with improved color intensity
WO2024115106A1 (en) 2022-11-29 2024-06-06 Unilever Ip Holdings B.V. Composition
DE102022131732A1 (en) 2022-11-30 2024-06-06 Henkel Ag & Co. Kgaa Improved washing performance through the use of a protease fused with a special adhesion promoter peptide
WO2024121070A1 (en) 2022-12-05 2024-06-13 Novozymes A/S Protease variants and polynucleotides encoding same
WO2024121057A1 (en) 2022-12-05 2024-06-13 Novozymes A/S A composition for removing body grime
DE102022213538A1 (en) 2022-12-13 2024-06-13 Henkel Ag & Co. Kgaa WASHING AND CLEANING PRODUCTS CONTAINING PROTEASE
DE102022213537A1 (en) 2022-12-13 2024-06-13 Henkel Ag & Co. Kgaa WASHING AND CLEANING PRODUCTS CONTAINING PROTEASE
WO2024126483A1 (en) 2022-12-14 2024-06-20 Novozymes A/S Improved lipase (gcl1) variants
EP4389864A1 (en) 2022-12-20 2024-06-26 Basf Se Cutinases
WO2024131880A2 (en) 2022-12-23 2024-06-27 Novozymes A/S Detergent composition comprising catalase and amylase
DE102023200106A1 (en) 2023-01-10 2024-07-11 Henkel Ag & Co. Kgaa ENZYME-CONTAINING WASHING AND CLEANING AGENTS
WO2024156628A1 (en) 2023-01-23 2024-08-02 Novozymes A/S Cleaning compositions and uses thereof
EP4414443A1 (en) 2023-02-09 2024-08-14 Henkel AG & Co. KGaA Cleaning composition comprising polyesterase
DE102023201692A1 (en) 2023-02-24 2024-08-29 Henkel Ag & Co. Kgaa WASHING AND CLEANING PRODUCTS WITH DISPERSIN AND FRAGRANCE
DE102023201696A1 (en) 2023-02-24 2024-08-29 Henkel Ag & Co. Kgaa WASHING AND CLEANING PRODUCTS WITH DISPERSIN
DE102023201695A1 (en) 2023-02-24 2024-08-29 Henkel Ag & Co. Kgaa WASHING AND CLEANING PRODUCTS WITH DISPERSIN
WO2024194190A1 (en) 2023-03-17 2024-09-26 Unilever Ip Holdings B.V. Composition
WO2024194245A1 (en) 2023-03-21 2024-09-26 Novozymes A/S Detergent compositions based on biosurfactants
WO2024194098A1 (en) 2023-03-21 2024-09-26 Unilever Ip Holdings B.V. Detergent unit dose
CN117106023B (en) * 2023-10-25 2023-12-15 深圳市维琪科技股份有限公司 Pentapeptide compounds, compositions and uses thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4081328A (en) * 1975-10-23 1978-03-28 Stanford Research Institute Production of cellulase by a thermophilic thielavia terrestris
US4966850A (en) * 1987-01-21 1990-10-30 Forintek Canada Corp. Production of thermostable xylanase and cellulase
US5314637A (en) * 1990-07-24 1994-05-24 Showa Denko K.K. Detergent comprising isolated cellulase from bacillus ferm bp-3431 or a mutant strain thereof, surfactant and builder
US6001639A (en) * 1995-03-17 1999-12-14 Novo Nordisk A/S Endoglucanases

Family Cites Families (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1368599A (en) 1970-09-29 1974-10-02 Unilever Ltd Softening compositions
GB1483591A (en) 1973-07-23 1977-08-24 Novo Industri As Process for coating water soluble or water dispersible particles by means of the fluid bed technique
GB1590432A (en) 1976-07-07 1981-06-03 Novo Industri As Process for the production of an enzyme granulate and the enzyme granuate thus produced
US4081326A (en) * 1976-10-07 1978-03-28 The Board Of Trustees Of The University Of Alabama A method for removing maltotetraose and lower saccharides from solution
US4203570A (en) 1978-08-23 1980-05-20 The Western States Machine Company Power-operated loading gate for centrifugal machines incorporating an auxiliary drive device
GB2094826B (en) 1981-03-05 1985-06-12 Kao Corp Cellulase enzyme detergent composition
US4546082A (en) 1982-06-17 1985-10-08 Regents Of The Univ. Of California E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins
US4599311A (en) 1982-08-13 1986-07-08 Kawasaki Glenn H Glycolytic promotersfor regulated protein expression: protease inhibitor
JPS60501140A (en) 1983-04-22 1985-07-25 アムジエン Secretion of exogenous polypeptides by yeast
NZ207926A (en) 1983-04-25 1988-04-29 Genentech Inc Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast
US4870008A (en) 1983-08-12 1989-09-26 Chiron Corporation Secretory expression in eukaryotes
US4931373A (en) 1984-05-25 1990-06-05 Zymogenetics, Inc. Stable DNA constructs for expression of α-1 antitrypsin
DK263584D0 (en) 1984-05-29 1984-05-29 Novo Industri As ENZYMOUS GRANULATES USED AS DETERGENT ADDITIVES
DK58285D0 (en) 1984-05-30 1985-02-08 Novo Industri As PEPTIDES AND MANUFACTURING AND USING THEREOF
US4766073A (en) 1985-02-25 1988-08-23 Zymogenetics Inc. Expression of biologically active PDGF analogs in eucaryotic cells
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP0233910B1 (en) 1985-07-16 1990-09-19 INNOFINANCE Altalános Innovácios Pénzintézet Plant protecting composition
HU206897B (en) 1985-10-25 1993-01-28 Zymogenetics Inc Process for utilizing bar-1 gen for selecting strange proteins
US4882279A (en) 1985-10-25 1989-11-21 Phillips Petroleum Company Site selective genomic modification of yeast of the genus pichia
AU607690B2 (en) 1985-12-24 1991-03-14 Marion Laboratories, Inc. Use of synthetic sulfated saccharides to enhance wound healing
US4935349A (en) 1986-01-17 1990-06-19 Zymogenetics, Inc. Expression of higher eucaryotic genes in aspergillus
EG18543A (en) 1986-02-20 1993-07-30 Albright & Wilson Protected enzyme systems
DK122686D0 (en) 1986-03-17 1986-03-17 Novo Industri As PREPARATION OF PROTEINS
FR2604198B1 (en) 1986-09-22 1989-07-07 Du Pin Cellulose PROCESS FOR TREATING A PAPER PULP WITH AN ENZYMATIC SOLUTION.
DK463887D0 (en) 1987-09-07 1987-09-07 Novo Industri As GAERLEADER
US4832864A (en) 1987-09-15 1989-05-23 Ecolab Inc. Compositions and methods that introduce variations in color density into cellulosic fabrics, particularly indigo dyed denim
US5137819A (en) 1988-07-08 1992-08-11 University Of British Columbia Cellulose binding fusion proteins for immobilization and purification of polypeptides
US5037743A (en) 1988-08-05 1991-08-06 Zymogenetics, Inc. BAR1 secretion signal
US5006126A (en) 1988-09-15 1991-04-09 Ecolab Inc. Cellulase compositions and methods that introduce variations in color density into cellulosic fabrics, particularly indigo dyed denim
DK16490D0 (en) 1990-01-19 1990-01-19 Novo Nordisk As ENZYME
DK80390D0 (en) 1990-03-29 1990-03-29 Novo Nordisk As
DK80290D0 (en) 1990-03-29 1990-03-29 Novo Nordisk As
EP0531372B2 (en) 1990-05-09 2004-04-14 Novozymes A/S A cellulase preparation comprising an endoglucanase enzyme
DK115890D0 (en) 1990-05-09 1990-05-09 Novo Nordisk As ENZYME
SK46293A3 (en) 1990-09-28 1994-01-12 Procter & Gamble Polyhydroxy fatty acid amide surfactants to enhance enzyme performance
US5525507A (en) * 1990-10-05 1996-06-11 Genencor International, Inc. Methods for treating cotton-containing fabric with cellulase composition containing endoglucanase component and which is free of all CBH I component
US5475101A (en) 1990-10-05 1995-12-12 Genencor International, Inc. DNA sequence encoding endoglucanase III cellulase
WO1992006221A1 (en) 1990-10-05 1992-04-16 Genencor International, Inc. Methods for treating cotton-containing fabrics with cellulase
DK300090D0 (en) 1990-12-19 1990-12-19 Novo Nordisk As PROCEDURE FOR PREPARING LEADER SEQUENCES
EP0495257B1 (en) 1991-01-16 2002-06-12 The Procter & Gamble Company Compact detergent compositions with high activity cellulase
EP0507723A1 (en) 1991-04-02 1992-10-07 Novo Nordisk A/S Xylanase, corresponding recombinant DNA sequence, xylanase containing agent, and use of the agent
ES2083560T3 (en) * 1991-04-12 1996-04-16 Procter & Gamble COMPACT DETERGENT COMPOSITION CONTAINING POLYVINYLPYROLIDONE.
DK73891D0 (en) 1991-04-22 1991-04-22 Novo Nordisk As ENZYME TREATMENT
EP0511456A1 (en) 1991-04-30 1992-11-04 The Procter & Gamble Company Liquid detergents with aromatic borate ester to inhibit proteolytic enzyme
ES2085024T3 (en) 1991-04-30 1996-05-16 Procter & Gamble LIQUID DETERGENTS REINFORCED WITH BORICO-POLYOL ACID COMPLEX TO INHIBIT THE PROTEOLYTIC ENZYME.
ATE169678T1 (en) * 1991-05-01 1998-08-15 Novo Nordisk As STABILIZED ENZYMES
EP0540784B1 (en) * 1991-11-06 2000-01-19 The Procter & Gamble Company Dye transfer inhibiting compositions
MX9206979A (en) 1991-12-04 1993-07-01 Novo Nordisk As CLONING METHOD FOR PROTEINS IN YEAST
DK41992D0 (en) 1992-03-27 1992-03-27 Novo Nordisk As
DE69304520D1 (en) 1992-04-06 1996-10-10 Novo Nordisk As METHOD FOR UNLEASHING AND DEHARNING CELLULOSE TISSUE
ATE262035T1 (en) * 1992-10-06 2004-04-15 Novozymes As CELLULOSE VARIANTS
ES2202320T3 (en) 1992-12-23 2004-04-01 Novozymes A/S ENZYME WITH ENDOGLUCANASA ACTIVITY.
US5565006A (en) 1993-01-20 1996-10-15 Novo Nordisk A/S Method for the treatment of dyed fabric
HUT70470A (en) 1993-05-10 1995-10-30 Gist Brocades Nv Combined action of endoglucanases and cellobiohydrolases
DK81293D0 (en) * 1993-07-06 1993-07-06 Novo Nordisk As ENZYME
AU7069294A (en) * 1993-07-12 1995-02-13 Novo Nordisk A/S A detergent composition comprising two cellulase components
US5861271A (en) 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
SE502163C2 (en) 1993-12-21 1995-09-04 Sunds Defibrator Ind Ab Device for mixing treatment medium in a pulp suspension
DE69534513T2 (en) 1994-03-08 2006-07-27 Novozymes A/S NOVEL ALKALINE CELLULASES
AU1946895A (en) 1994-03-28 1995-10-17 Novo Nordisk A/S A modified cellulase and an enzyme preparation comprising a modified cellulase
HUT77195A (en) 1994-12-05 1998-03-02 Novo Nordisk A/S A method of obtaining a cellulosic textile fabric with reduced tendency to pilling formation
EP0843041A1 (en) * 1996-11-13 1998-05-20 Novo Nordisk A/S Garments with considerable variation in abrasion level and process for its production using cellulolytic enzymes
ES2637008T3 (en) 2005-12-22 2017-10-10 Ab Enzymes Oy New enzymes
CN101910405A (en) 2007-11-01 2010-12-08 诺维信股份有限公司 Methods of reducing the inhibitory effect of a tannin of the enzymatic hydrolysis of cellulosic material

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4081328A (en) * 1975-10-23 1978-03-28 Stanford Research Institute Production of cellulase by a thermophilic thielavia terrestris
US4966850A (en) * 1987-01-21 1990-10-30 Forintek Canada Corp. Production of thermostable xylanase and cellulase
US5314637A (en) * 1990-07-24 1994-05-24 Showa Denko K.K. Detergent comprising isolated cellulase from bacillus ferm bp-3431 or a mutant strain thereof, surfactant and builder
US6001639A (en) * 1995-03-17 1999-12-14 Novo Nordisk A/S Endoglucanases

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090298145A1 (en) * 2008-04-10 2009-12-03 Kabushiki Kaisha Toyota Chuo Kenkyusho Acid-resistance endoglucanase and the use of thereof
US7960511B2 (en) 2008-04-10 2011-06-14 Kabushiki Kaisha Toyota Chuo Kenkyusho Acid-resistance endoglucanase and the use of thereof
US8609387B2 (en) 2008-12-30 2013-12-17 Ab Enzymes Oy Fungal endoglucanases, their production and use
US8802423B2 (en) * 2010-12-30 2014-08-12 Novozymes A/S Method for treating textile with endoglucanase
WO2013138288A1 (en) 2012-03-16 2013-09-19 Monosol, Llc. Water soluble compositions incorporating enzymes, and method of making same
US10087401B2 (en) 2012-03-16 2018-10-02 Monosol, Llc Water soluble compositions incorporating enzymes, and method of making same
EP3354716A1 (en) 2012-03-16 2018-08-01 Monosol, LLC Water soluble compositions incorporating enzymes, and method of making same
US9908675B2 (en) 2012-04-16 2018-03-06 Monosol, Llc Powdered pouch and method of making same
US9394092B2 (en) 2012-04-16 2016-07-19 Monosol, Llc Powdered pouch and method of making same
WO2013158364A1 (en) 2012-04-16 2013-10-24 Monosol, Llc Powdered pouch and method of making same
US10696460B2 (en) 2012-04-16 2020-06-30 Monosol, Llc Powdered pouch and method of making same
US11753222B2 (en) 2012-04-16 2023-09-12 Monosol, Llc Powdered pouch and method of making same
US9850512B2 (en) 2013-03-15 2017-12-26 The Research Foundation For The State University Of New York Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield
US9951363B2 (en) 2014-03-14 2018-04-24 The Research Foundation for the State University of New York College of Environmental Science and Forestry Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects
US11104497B2 (en) 2014-10-03 2021-08-31 Monosol, Llc Degradable materials and packaging made from same
US11884467B2 (en) 2014-10-03 2024-01-30 Monosol, Llc Degradable materials and packaging made from same

Also Published As

Publication number Publication date
MX9706974A (en) 1997-11-29
CN102146362A (en) 2011-08-10
CN1182451A (en) 1998-05-20
US20030054539A1 (en) 2003-03-20
EP2431462A2 (en) 2012-03-21
AU4939496A (en) 1996-10-08
US6855531B2 (en) 2005-02-15
EP0815209B1 (en) 2006-01-04
EP2431462A3 (en) 2012-05-23
US7226773B2 (en) 2007-06-05
EP0815209B2 (en) 2015-02-25
DE815209T1 (en) 1998-06-25
CA2214116C (en) 2011-05-24
AR030675A2 (en) 2003-09-03
BRPI9607646B1 (en) 2016-07-05
CN102080070B (en) 2016-01-20
CN101955921A (en) 2011-01-26
DE69635700T3 (en) 2015-05-21
US6001639A (en) 1999-12-14
US6387690B1 (en) 2002-05-14
DE69635700D1 (en) 2006-03-30
EP1683860A3 (en) 2007-05-02
WO1996029397A1 (en) 1996-09-26
US20100107342A1 (en) 2010-05-06
EP0815209A1 (en) 1998-01-07
JPH11502701A (en) 1999-03-09
US9023620B2 (en) 2015-05-05
US8642730B2 (en) 2014-02-04
ATE315083T1 (en) 2006-02-15
BR9607646A (en) 1998-06-16
EP1683860B1 (en) 2013-10-23
CA2214116A1 (en) 1996-09-26
CN102080070A (en) 2011-06-01
AU715423B2 (en) 2000-02-03
JP3360830B2 (en) 2003-01-07
DE69635700T2 (en) 2006-09-14
US20130330781A1 (en) 2013-12-12
NZ303162A (en) 2000-01-28
EP1683860A2 (en) 2006-07-26
US20050070003A1 (en) 2005-03-31
CN101173263A (en) 2008-05-07

Similar Documents

Publication Publication Date Title
US9023620B2 (en) Method of providing color clarification of laundry
EP0788541B1 (en) Enzyme preparation with endoglucanase activity
ES2384276T3 (en) Cellulase fusion proteins and their use
JP5357514B2 (en) Chrysosporium cellulase and method of use
US5912157A (en) Alkaline cellulases
EP1632557A2 (en) Novel alkaline cellulases
BRPI0620318B1 (en) endoglycanase fusion protein, isolated polynucleotide, expression vector, trichoderma reesei host cell, enzyme preparation, detergent composition, escherichia coli strain, and processes for producing an endoglycanase polypeptide, for bioassay, for biofinishing, for textile processing containing cellulosic fiber, for treating pulp or wood-derived fiber, and for improving feed quality
CA2201871C (en) An enzyme and enzyme preparation with endoglucanase activity

Legal Events

Date Code Title Description
STCB Information on status: application discontinuation

Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION