MX9206979A - CLONING METHOD FOR PROTEINS IN YEAST - Google Patents

CLONING METHOD FOR PROTEINS IN YEAST

Info

Publication number
MX9206979A
MX9206979A MX9206979A MX9206979A MX9206979A MX 9206979 A MX9206979 A MX 9206979A MX 9206979 A MX9206979 A MX 9206979A MX 9206979 A MX9206979 A MX 9206979A MX 9206979 A MX9206979 A MX 9206979A
Authority
MX
Mexico
Prior art keywords
interest
proteins
protein
host cells
yeast
Prior art date
Application number
MX9206979A
Other languages
Spanish (es)
Inventor
Henrik Dalboge
Hans Peter Heldt-Hansen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of MX9206979A publication Critical patent/MX9206979A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38654—Preparations containing enzymes, e.g. protease or amylase containing oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

Abstract

Esta invención describe un método de selección para una secuencia de ADN que codifica para una proteína de interés, el método comprende: (a) Clonar, en vectores adecuados, una biblioteca de ADN de un organismo que se sospecha produce una o más proteínas de interes. (b) Transformar células huésped de levadura adecuadas con los vetores. (c) Cultivar las células huesped bajo condiciones adecuadas,para expresar cualquier proteína de interés codificada por un clon en la biblioteca ADN y (d) Seleccionar clones positivos, determinando cualquier actividad de una proteína expresada en el paso (c).This invention describes a selection method for a DNA sequence encoding a protein of interest, the method comprising: (a) Cloning, into suitable vectors, a DNA library from an organism suspected of producing one or more proteins of interest . (b) Transform suitable yeast host cells with the vetor cells. (c) Culture the host cells under suitable conditions, to express any protein of interest encoded by a clone in the DNA library and (d) Select positive clones, determining any activity of a protein expressed in step (c).

MX9206979A 1991-12-04 1991-12-04 CLONING METHOD FOR PROTEINS IN YEAST MX9206979A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK9100378 1991-12-04
DK9100379 1991-12-04

Publications (1)

Publication Number Publication Date
MX9206979A true MX9206979A (en) 1993-07-01

Family

ID=26068344

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9206979A MX9206979A (en) 1991-12-04 1991-12-04 CLONING METHOD FOR PROTEINS IN YEAST

Country Status (7)

Country Link
EP (1) EP0618974A1 (en)
JP (1) JPH08504560A (en)
BR (1) BR9206866A (en)
CA (1) CA2124939A1 (en)
FI (1) FI942644A0 (en)
MX (1) MX9206979A (en)
WO (1) WO1993011249A1 (en)

Families Citing this family (32)

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US5919691A (en) * 1994-10-06 1999-07-06 Novo Nordisk A/S Enzyme and enzyme preparation with endoglucanase activity
JPH10507642A (en) * 1994-10-26 1998-07-28 ノボ ノルディスク アクティーゼルスカブ Enzymes with lipolytic activity
MX9706974A (en) 1995-03-17 1997-11-29 Novo Nordisk As Novel endoglucanases.
US6410701B1 (en) * 1995-05-05 2002-06-25 Human Genome Sciences, Inc. Human neuropeptide receptor
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
WO1997013853A2 (en) * 1995-10-13 1997-04-17 Gist-Brocades B.V. Protein detection
US6723549B2 (en) 1995-10-17 2004-04-20 Ab Enzymes Oy Cellulases, the genes encoding them and uses thereof
US6184019B1 (en) 1995-10-17 2001-02-06 Röhm Enzyme Finland OY Cellulases, the genes encoding them and uses thereof
AU1438497A (en) * 1996-01-29 1997-08-22 Novo Nordisk A/S Process for removal or bleaching of soiling or stains from cellulosic fabric
CN1209838A (en) * 1996-01-29 1999-03-03 诺沃挪第克公司 Process for desizing cellulosic fabric
WO1997043409A2 (en) * 1996-05-10 1997-11-20 Novo Nordisk A/S Method of providing novel dna sequences
AU2887897A (en) 1996-05-21 1997-12-09 Novo Nordisk A/S Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast
DK1616580T3 (en) 1996-07-05 2008-01-14 Novozymes As The transcription factor
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
JP4463878B2 (en) * 1996-10-28 2010-05-19 ノボザイムス アクティーゼルスカブ Extracellular expression of cellulose binding domains using Bacillus bacteria
AU5187898A (en) 1996-12-09 1998-07-03 Novo Nordisk A/S Reduction of phosphorus containing components in edible oils comprising a high amount of non-hydratable phosphorus by use of a phospholipase, a phospholipase from a filamentous fungus having phospholipase a and/or b activity
ATE367439T1 (en) * 1997-12-22 2007-08-15 Dsm Ip Assets Bv EXPRESSION CLONING IN FILAMENTOUS FUNGI
DK1058738T3 (en) * 1998-03-06 2002-03-11 Novozymes As Method of screening based on fluorescence polarization
EP2287318B1 (en) 1998-06-10 2014-01-22 Novozymes A/S Mannanases
EP1124948A1 (en) * 1998-10-28 2001-08-22 Novozymes A/S Method for generating a gene library
US6723504B1 (en) 1998-10-28 2004-04-20 Novozymes A/S Method for generating a gene library
US7220542B2 (en) 2000-07-17 2007-05-22 Van Den Brink Johannes Maarten Expression cloning in filamentous fungi
EP1059351A1 (en) * 1999-06-11 2000-12-13 The Procter & Gamble Company Non-aqueous liquid detergent compositions comprising a borate-releasing compound and a mannanase
IL146935A0 (en) * 2000-04-13 2002-08-14 Emalfarb Mark Aaron High-throughput screening of expressed dna libraries in filamentous fungi
KR20020026456A (en) 2000-04-13 2002-04-10 에말파브 마크 아론 High-throughput screening of expressed dna libraries in filamentous fungi
NZ528260A (en) 2001-05-18 2005-09-30 Danisco Method of improving dough and bread quality with the addition of an enzyme that hydrolyses a glycolipid and a phospholipid and incapable of hydrolysing a triglyceride or monoglyceride
WO2003070957A2 (en) * 2002-02-20 2003-08-28 Novozymes A/S Plant polypeptide production
DK2305703T3 (en) 2004-01-30 2014-06-16 Novozymes Inc Polypeptides with cellulolytic enhancing activity and polynucleotides encoding them
MX2007000626A (en) 2004-07-16 2007-03-07 Danisco Protein.
WO2007109441A2 (en) 2006-03-20 2007-09-27 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
US8680252B2 (en) 2006-12-10 2014-03-25 Dyadic International (Usa), Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
CA2736661A1 (en) 2007-09-07 2009-03-12 Dyadic International, Inc. Novel fungal enzymes

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK187280A (en) * 1980-04-30 1981-10-31 Novo Industri As RUIT REDUCING AGENT FOR A COMPLETE LAUNDRY
WO1989009259A1 (en) * 1988-03-24 1989-10-05 Novo-Nordisk A/S A cellulase preparation
AU639570B2 (en) * 1990-05-09 1993-07-29 Novozymes A/S A cellulase preparation comprising an endoglucanase enzyme
DE69114500T2 (en) * 1990-07-13 1996-06-13 Transkaryotic Therapies Inc METHOD FOR SCANING A LIBRARY.

Also Published As

Publication number Publication date
FI942644A (en) 1994-06-03
WO1993011249A1 (en) 1993-06-10
FI942644A0 (en) 1994-06-03
EP0618974A1 (en) 1994-10-12
CA2124939A1 (en) 1993-06-10
JPH08504560A (en) 1996-05-21
BR9206866A (en) 1995-11-21

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