SK287865B6 - Hirudin precursor comprising signal sequence, method for the preparation of Leu-hirudin and use of precursor - Google Patents

Hirudin precursor comprising signal sequence, method for the preparation of Leu-hirudin and use of precursor Download PDF

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Publication number
SK287865B6
SK287865B6 SK362-2002A SK3622002A SK287865B6 SK 287865 B6 SK287865 B6 SK 287865B6 SK 3622002 A SK3622002 A SK 3622002A SK 287865 B6 SK287865 B6 SK 287865B6
Authority
SK
Slovakia
Prior art keywords
hirudin
leu
sequence
precursor
artificial sequence
Prior art date
Application number
SK362-2002A
Other languages
English (en)
Slovak (sk)
Other versions
SK3622002A3 (en
Inventor
Paul Habermann
Rudolf Bender
Original Assignee
Sanofi-Aventis Deutschland Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi-Aventis Deutschland Gmbh filed Critical Sanofi-Aventis Deutschland Gmbh
Publication of SK3622002A3 publication Critical patent/SK3622002A3/sk
Publication of SK287865B6 publication Critical patent/SK287865B6/sk

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • C12N15/625DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81Protease inhibitors
    • C07K14/815Protease inhibitors from leeches, e.g. hirudin, eglin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Hematology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Diabetes (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
SK362-2002A 1999-09-18 2000-09-01 Hirudin precursor comprising signal sequence, method for the preparation of Leu-hirudin and use of precursor SK287865B6 (sk)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19944870A DE19944870A1 (de) 1999-09-18 1999-09-18 Signalsequenzen zur Herstellung von Leu-Hirudin über Sekretion durch E. coli in das Kulturmedium
PCT/EP2000/008537 WO2001021662A1 (de) 1999-09-18 2000-09-01 Signalsequenzen zur herstellung von leu-hirudin über sekretion durch e. coli in das kulturmedium

Publications (2)

Publication Number Publication Date
SK3622002A3 SK3622002A3 (en) 2003-06-03
SK287865B6 true SK287865B6 (sk) 2012-02-03

Family

ID=7922547

Family Applications (1)

Application Number Title Priority Date Filing Date
SK362-2002A SK287865B6 (sk) 1999-09-18 2000-09-01 Hirudin precursor comprising signal sequence, method for the preparation of Leu-hirudin and use of precursor

Country Status (29)

Country Link
US (1) US7455987B1 (ko)
EP (1) EP1216259B1 (ko)
JP (1) JP4669181B2 (ko)
KR (1) KR100737189B1 (ko)
CN (2) CN1269838C (ko)
AR (1) AR025660A1 (ko)
AT (1) ATE357458T1 (ko)
AU (1) AU775923B2 (ko)
BR (1) BR0014519A (ko)
CA (1) CA2385287C (ko)
CZ (1) CZ2002899A3 (ko)
DE (2) DE19944870A1 (ko)
DK (1) DK1216259T3 (ko)
EE (1) EE05187B1 (ko)
HK (2) HK1049172A1 (ko)
HR (1) HRP20020226B1 (ko)
HU (1) HUP0202596A3 (ko)
IL (2) IL148624A0 (ko)
ME (1) MEP27908A (ko)
MX (1) MXPA02002469A (ko)
NO (1) NO329723B1 (ko)
NZ (1) NZ517825A (ko)
PL (1) PL205117B1 (ko)
RS (1) RS50435B (ko)
RU (1) RU2261867C2 (ko)
SK (1) SK287865B6 (ko)
TR (2) TR200200667T2 (ko)
WO (1) WO2001021662A1 (ko)
ZA (1) ZA200202106B (ko)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7638618B2 (en) 2001-02-20 2009-12-29 Sanofi-Aventis Deutschland Gmbh Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest
US7202059B2 (en) 2001-02-20 2007-04-10 Sanofi-Aventis Deutschland Gmbh Fusion proteins capable of being secreted into a fermentation medium
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
EP2327718B1 (en) 2003-11-21 2016-03-23 Pfenex, Inc. Improved expression systems with SEC-system secretion
CN101031655A (zh) 2004-07-26 2007-09-05 陶氏环球技术公司 通过株工程改进蛋白表达的方法
DE102005046113A1 (de) * 2005-09-27 2007-03-29 Sanofi-Aventis Deutschland Gmbh Verfahren zur Amidierung von Polypetiden mit C-terminalen basischen Aminosäuren unter Verwendung spezifischer Endoproteasen
DE102006044841A1 (de) 2006-09-22 2008-04-03 Wacker Chemie Ag Signalpeptid zur Produktion von rekombinanten Proteinen
KR101491867B1 (ko) 2007-01-31 2015-02-10 피페넥스 인크. 증가된 발현을 위한 박테리아 리더 서열
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
WO2008134461A2 (en) 2007-04-27 2008-11-06 Dow Global Technologies, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
KR20160029731A (ko) 2013-03-12 2016-03-15 더 제너럴 하스피탈 코포레이션 변형된 뮐러관 억제 물질(mis) 단백질 및 질환 치료를 위한 그 용도
EP3079712B1 (en) 2013-12-11 2022-02-02 The General Hospital Corporation Use of mullerian inhibiting substance (mis) proteins for contraception and ovarian reserve preservation
CN108220369B (zh) * 2017-12-04 2021-03-12 广东双骏生物科技有限公司 一种生产重组水蛭素的方法
CN108359689B (zh) * 2018-01-19 2021-03-23 华中农业大学 利用抗病基因识别无毒效应蛋白以鉴定具有分泌功能信号肽的方法
CN115572329B (zh) * 2021-06-21 2024-02-06 王大勇 一组活性增强代谢较慢的菲牛蛭基因重组水蛭素及其制备方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3429430A1 (de) * 1984-08-10 1986-02-20 Hoechst Ag, 6230 Frankfurt Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens
ES2055149T3 (es) * 1985-04-11 1994-08-16 Hoechst Ag Derivado de hirudina.
US5084384A (en) * 1987-04-23 1992-01-28 Monsanto Company Secretion of insulin-like growth factor-I
AU604925B2 (en) * 1988-02-23 1991-01-03 Schering Aktiengesellschaft A hirudin derivative
DE4009268A1 (de) * 1990-03-22 1991-09-26 Consortium Elektrochem Ind Sekretion von hirudinderivaten
CA2255396A1 (en) * 1990-11-08 1992-05-09 Nippon Mining Company Limited Hirudin analog, method of manufacturing thereof and anti-coagulant composition, and secretion vector, transformed microorganisms containing said vector and manufacturing method ofproducts which is produced from said microorganism
JPH07119237B2 (ja) * 1990-11-08 1995-12-20 株式会社ジャパンエナジー ヒルジン変異体,その製造法及び抗凝血剤
NZ243084A (en) * 1991-06-12 1995-08-28 Regeneron Pharma Production and recovery of recombinant neurotrophins, genes for a modified lamb signal sequence, fusion genes and plasmids
US5389529A (en) * 1991-06-12 1995-02-14 Regeneron Pharmaceuticals, Inc. Modified lamβ signal sequence and processes for producing recombinant neurotrophins
DE4140381A1 (de) 1991-12-07 1993-06-09 Hoechst Ag, 6230 Frankfurt, De Neue synthetische isohirudine mit verbesserter stabilitaet

Also Published As

Publication number Publication date
WO2001021662A1 (de) 2001-03-29
CZ2002899A3 (cs) 2002-06-12
DE50014189D1 (de) 2007-05-03
CN1269838C (zh) 2006-08-16
HRP20020226A2 (en) 2004-04-30
CA2385287C (en) 2010-10-19
AR025660A1 (es) 2002-12-11
CN1192037C (zh) 2005-03-09
DK1216259T3 (da) 2007-07-02
HUP0202596A2 (hu) 2002-12-28
KR20020034188A (ko) 2002-05-08
AU775923B2 (en) 2004-08-19
EP1216259B1 (de) 2007-03-21
AU6842100A (en) 2001-04-24
IL148624A0 (en) 2002-09-12
JP2003510041A (ja) 2003-03-18
ZA200202106B (en) 2003-01-09
SK3622002A3 (en) 2003-06-03
YU14602A (sh) 2005-07-19
BR0014519A (pt) 2002-06-11
NO20021254D0 (no) 2002-03-13
RS50435B (sr) 2009-12-31
US7455987B1 (en) 2008-11-25
DE19944870A1 (de) 2001-03-29
NO20021254L (no) 2002-05-07
PL354444A1 (en) 2004-01-12
PL205117B1 (pl) 2010-03-31
CN1550502A (zh) 2004-12-01
NO329723B1 (no) 2010-12-06
HK1068352A1 (en) 2005-04-29
EE05187B1 (et) 2009-06-15
JP4669181B2 (ja) 2011-04-13
RU2261867C2 (ru) 2005-10-10
TR200501541T2 (tr) 2005-06-21
KR100737189B1 (ko) 2007-07-10
EP1216259A1 (de) 2002-06-26
RU2002110278A (ru) 2004-03-27
CA2385287A1 (en) 2001-03-29
MEP27908A (en) 2010-10-10
ATE357458T1 (de) 2007-04-15
NZ517825A (en) 2004-07-30
HUP0202596A3 (en) 2005-01-28
MXPA02002469A (es) 2002-08-28
IL148624A (en) 2009-08-03
HRP20020226B1 (en) 2010-08-31
HK1049172A1 (en) 2003-05-02
EE200200139A (et) 2003-06-16
CN1374970A (zh) 2002-10-16
TR200200667T2 (tr) 2002-06-21

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MM4A Patent lapsed due to non-payment of maintenance fees

Effective date: 20120901