SK287865B6 - Hirudin precursor comprising signal sequence, method for the preparation of Leu-hirudin and use of precursor - Google Patents
Hirudin precursor comprising signal sequence, method for the preparation of Leu-hirudin and use of precursor Download PDFInfo
- Publication number
- SK287865B6 SK287865B6 SK362-2002A SK3622002A SK287865B6 SK 287865 B6 SK287865 B6 SK 287865B6 SK 3622002 A SK3622002 A SK 3622002A SK 287865 B6 SK287865 B6 SK 287865B6
- Authority
- SK
- Slovakia
- Prior art keywords
- hirudin
- leu
- sequence
- precursor
- artificial sequence
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Hematology (AREA)
- Animal Behavior & Ethology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Veterinary Medicine (AREA)
- Diabetes (AREA)
- Pharmacology & Pharmacy (AREA)
- General Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19944870A DE19944870A1 (de) | 1999-09-18 | 1999-09-18 | Signalsequenzen zur Herstellung von Leu-Hirudin über Sekretion durch E. coli in das Kulturmedium |
| PCT/EP2000/008537 WO2001021662A1 (de) | 1999-09-18 | 2000-09-01 | Signalsequenzen zur herstellung von leu-hirudin über sekretion durch e. coli in das kulturmedium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| SK3622002A3 SK3622002A3 (en) | 2003-06-03 |
| SK287865B6 true SK287865B6 (sk) | 2012-02-03 |
Family
ID=7922547
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SK362-2002A SK287865B6 (sk) | 1999-09-18 | 2000-09-01 | Hirudin precursor comprising signal sequence, method for the preparation of Leu-hirudin and use of precursor |
Country Status (29)
| Country | Link |
|---|---|
| US (1) | US7455987B1 (ko) |
| EP (1) | EP1216259B1 (ko) |
| JP (1) | JP4669181B2 (ko) |
| KR (1) | KR100737189B1 (ko) |
| CN (2) | CN1269838C (ko) |
| AR (1) | AR025660A1 (ko) |
| AT (1) | ATE357458T1 (ko) |
| AU (1) | AU775923B2 (ko) |
| BR (1) | BR0014519A (ko) |
| CA (1) | CA2385287C (ko) |
| CZ (1) | CZ2002899A3 (ko) |
| DE (2) | DE19944870A1 (ko) |
| DK (1) | DK1216259T3 (ko) |
| EE (1) | EE05187B1 (ko) |
| HK (2) | HK1049172A1 (ko) |
| HR (1) | HRP20020226B1 (ko) |
| HU (1) | HUP0202596A3 (ko) |
| IL (2) | IL148624A0 (ko) |
| ME (1) | MEP27908A (ko) |
| MX (1) | MXPA02002469A (ko) |
| NO (1) | NO329723B1 (ko) |
| NZ (1) | NZ517825A (ko) |
| PL (1) | PL205117B1 (ko) |
| RS (1) | RS50435B (ko) |
| RU (1) | RU2261867C2 (ko) |
| SK (1) | SK287865B6 (ko) |
| TR (2) | TR200200667T2 (ko) |
| WO (1) | WO2001021662A1 (ko) |
| ZA (1) | ZA200202106B (ko) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7638618B2 (en) | 2001-02-20 | 2009-12-29 | Sanofi-Aventis Deutschland Gmbh | Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest |
| US7202059B2 (en) | 2001-02-20 | 2007-04-10 | Sanofi-Aventis Deutschland Gmbh | Fusion proteins capable of being secreted into a fermentation medium |
| US9453251B2 (en) | 2002-10-08 | 2016-09-27 | Pfenex Inc. | Expression of mammalian proteins in Pseudomonas fluorescens |
| EP2327718B1 (en) | 2003-11-21 | 2016-03-23 | Pfenex, Inc. | Improved expression systems with SEC-system secretion |
| CN101031655A (zh) | 2004-07-26 | 2007-09-05 | 陶氏环球技术公司 | 通过株工程改进蛋白表达的方法 |
| DE102005046113A1 (de) * | 2005-09-27 | 2007-03-29 | Sanofi-Aventis Deutschland Gmbh | Verfahren zur Amidierung von Polypetiden mit C-terminalen basischen Aminosäuren unter Verwendung spezifischer Endoproteasen |
| DE102006044841A1 (de) | 2006-09-22 | 2008-04-03 | Wacker Chemie Ag | Signalpeptid zur Produktion von rekombinanten Proteinen |
| KR101491867B1 (ko) | 2007-01-31 | 2015-02-10 | 피페넥스 인크. | 증가된 발현을 위한 박테리아 리더 서열 |
| US9580719B2 (en) | 2007-04-27 | 2017-02-28 | Pfenex, Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
| WO2008134461A2 (en) | 2007-04-27 | 2008-11-06 | Dow Global Technologies, Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
| KR20160029731A (ko) | 2013-03-12 | 2016-03-15 | 더 제너럴 하스피탈 코포레이션 | 변형된 뮐러관 억제 물질(mis) 단백질 및 질환 치료를 위한 그 용도 |
| EP3079712B1 (en) | 2013-12-11 | 2022-02-02 | The General Hospital Corporation | Use of mullerian inhibiting substance (mis) proteins for contraception and ovarian reserve preservation |
| CN108220369B (zh) * | 2017-12-04 | 2021-03-12 | 广东双骏生物科技有限公司 | 一种生产重组水蛭素的方法 |
| CN108359689B (zh) * | 2018-01-19 | 2021-03-23 | 华中农业大学 | 利用抗病基因识别无毒效应蛋白以鉴定具有分泌功能信号肽的方法 |
| CN115572329B (zh) * | 2021-06-21 | 2024-02-06 | 王大勇 | 一组活性增强代谢较慢的菲牛蛭基因重组水蛭素及其制备方法 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3429430A1 (de) * | 1984-08-10 | 1986-02-20 | Hoechst Ag, 6230 Frankfurt | Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens |
| ES2055149T3 (es) * | 1985-04-11 | 1994-08-16 | Hoechst Ag | Derivado de hirudina. |
| US5084384A (en) * | 1987-04-23 | 1992-01-28 | Monsanto Company | Secretion of insulin-like growth factor-I |
| AU604925B2 (en) * | 1988-02-23 | 1991-01-03 | Schering Aktiengesellschaft | A hirudin derivative |
| DE4009268A1 (de) * | 1990-03-22 | 1991-09-26 | Consortium Elektrochem Ind | Sekretion von hirudinderivaten |
| CA2255396A1 (en) * | 1990-11-08 | 1992-05-09 | Nippon Mining Company Limited | Hirudin analog, method of manufacturing thereof and anti-coagulant composition, and secretion vector, transformed microorganisms containing said vector and manufacturing method ofproducts which is produced from said microorganism |
| JPH07119237B2 (ja) * | 1990-11-08 | 1995-12-20 | 株式会社ジャパンエナジー | ヒルジン変異体，その製造法及び抗凝血剤 |
| NZ243084A (en) * | 1991-06-12 | 1995-08-28 | Regeneron Pharma | Production and recovery of recombinant neurotrophins, genes for a modified lamb signal sequence, fusion genes and plasmids |
| US5389529A (en) * | 1991-06-12 | 1995-02-14 | Regeneron Pharmaceuticals, Inc. | Modified lamβ signal sequence and processes for producing recombinant neurotrophins |
| DE4140381A1 (de) | 1991-12-07 | 1993-06-09 | Hoechst Ag, 6230 Frankfurt, De | Neue synthetische isohirudine mit verbesserter stabilitaet |
-
1999
- 1999-09-18 DE DE19944870A patent/DE19944870A1/de not_active Withdrawn
-
2000
- 2000-09-01 CZ CZ2002899A patent/CZ2002899A3/cs unknown
- 2000-09-01 CN CNB2004100346716A patent/CN1269838C/zh not_active Expired - Fee Related
- 2000-09-01 EE EEP200200139A patent/EE05187B1/xx not_active IP Right Cessation
- 2000-09-01 BR BR0014519-0A patent/BR0014519A/pt not_active Application Discontinuation
- 2000-09-01 EP EP00956507A patent/EP1216259B1/de not_active Expired - Lifetime
- 2000-09-01 WO PCT/EP2000/008537 patent/WO2001021662A1/de active IP Right Grant
- 2000-09-01 AU AU68421/00A patent/AU775923B2/en not_active Ceased
- 2000-09-01 CA CA2385287A patent/CA2385287C/en not_active Expired - Fee Related
- 2000-09-01 ME MEP-279/08A patent/MEP27908A/xx unknown
- 2000-09-01 NZ NZ517825A patent/NZ517825A/en not_active IP Right Cessation
- 2000-09-01 KR KR1020027003577A patent/KR100737189B1/ko not_active IP Right Cessation
- 2000-09-01 CN CNB00812955XA patent/CN1192037C/zh not_active Expired - Fee Related
- 2000-09-01 RU RU2002110278/13A patent/RU2261867C2/ru not_active IP Right Cessation
- 2000-09-01 SK SK362-2002A patent/SK287865B6/sk not_active IP Right Cessation
- 2000-09-01 IL IL14862400A patent/IL148624A0/xx unknown
- 2000-09-01 MX MXPA02002469A patent/MXPA02002469A/es active IP Right Grant
- 2000-09-01 TR TR2002/00667T patent/TR200200667T2/xx unknown
- 2000-09-01 RS YUP-146/02A patent/RS50435B/sr unknown
- 2000-09-01 DK DK00956507T patent/DK1216259T3/da active
- 2000-09-01 PL PL354444A patent/PL205117B1/pl not_active IP Right Cessation
- 2000-09-01 AT AT00956507T patent/ATE357458T1/de not_active IP Right Cessation
- 2000-09-01 JP JP2001525235A patent/JP4669181B2/ja not_active Expired - Fee Related
- 2000-09-01 HU HU0202596A patent/HUP0202596A3/hu unknown
- 2000-09-01 TR TR2005/01541T patent/TR200501541T2/xx unknown
- 2000-09-01 DE DE50014189T patent/DE50014189D1/de not_active Expired - Lifetime
- 2000-09-14 AR ARP000104820A patent/AR025660A1/es active IP Right Grant
- 2000-09-18 US US09/664,326 patent/US7455987B1/en not_active Expired - Lifetime
-
2002
- 2002-03-11 IL IL148624A patent/IL148624A/en not_active IP Right Cessation
- 2002-03-13 NO NO20021254A patent/NO329723B1/no not_active IP Right Cessation
- 2002-03-14 ZA ZA200202106A patent/ZA200202106B/en unknown
- 2002-03-15 HR HR20020226A patent/HRP20020226B1/xx not_active IP Right Cessation
-
2003
- 2003-02-18 HK HK03101205A patent/HK1049172A1/xx not_active IP Right Cessation
-
2005
- 2005-01-13 HK HK05100336A patent/HK1068352A1/xx not_active IP Right Cessation
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | Patent lapsed due to non-payment of maintenance fees |
Effective date: 20120901 |