SG184833A1 - Methods of producing high titer, high purity virus stocks and methods of use thereof - Google Patents

Methods of producing high titer, high purity virus stocks and methods of use thereof Download PDF

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Publication number
SG184833A1
SG184833A1 SG2012075875A SG2012075875A SG184833A1 SG 184833 A1 SG184833 A1 SG 184833A1 SG 2012075875 A SG2012075875 A SG 2012075875A SG 2012075875 A SG2012075875 A SG 2012075875A SG 184833 A1 SG184833 A1 SG 184833A1
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SG
Singapore
Prior art keywords
virus
tcidso
less
tcids
sample
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SG2012075875A
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English (en)
Inventor
Damon R Asher
Amanda B Katz
Navid Z Khan
Ushma Mehta
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Emd Millipore Corp
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44148425&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=SG184833(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Emd Millipore Corp filed Critical Emd Millipore Corp
Publication of SG184833A1 publication Critical patent/SG184833A1/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02Recovery or purification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011Details
    • C12N2750/14011Parvoviridae
    • C12N2750/14311Parvovirus, e.g. minute virus of mice
    • C12N2750/14351Methods of production or purification of viral material

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG2012075875A 2010-04-14 2011-04-08 Methods of producing high titer, high purity virus stocks and methods of use thereof SG184833A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US32422010P 2010-04-14 2010-04-14
PCT/US2011/031747 WO2011130119A2 (en) 2010-04-14 2011-04-08 Methods of producing high titer, high purity virus stocks and methods of use thereof

Publications (1)

Publication Number Publication Date
SG184833A1 true SG184833A1 (en) 2012-11-29

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ID=44148425

Family Applications (1)

Application Number Title Priority Date Filing Date
SG2012075875A SG184833A1 (en) 2010-04-14 2011-04-08 Methods of producing high titer, high purity virus stocks and methods of use thereof

Country Status (9)

Country Link
US (1) US9644187B2 (enExample)
EP (1) EP2377927B1 (enExample)
JP (3) JP2013523175A (enExample)
KR (1) KR101549296B1 (enExample)
CN (1) CN102985536B (enExample)
BR (1) BR112012026095A2 (enExample)
ES (1) ES2736166T3 (enExample)
SG (1) SG184833A1 (enExample)
WO (1) WO2011130119A2 (enExample)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112012026095A2 (pt) 2010-04-14 2015-09-15 Emd Millipore Corp métodos de produção de estoques de vírus de alta pureza e altos títulos e métodos de uso dos mesmos.
DE102010046817A1 (de) * 2010-09-28 2012-03-29 Sartorius Stedim Biotech Gmbh Verfahren zur Abtrennung von Viren aus einem Kontaminanten enthaltenden flüssigen Medium
CN104160019A (zh) * 2012-03-13 2014-11-19 Emd密理博公司 使用离心辅助的感染以增加病毒滴度
CN104812893B (zh) * 2012-11-22 2018-08-03 旭化成医疗株式会社 高感染滴度的细小病毒的生产方法
EP2958995A4 (en) * 2013-02-22 2016-11-23 Agency Science Tech & Res CHROMATOGRAPHIC CLEANING OF VIRUS PREPARATIONS WITH NEGATIVELY LOADED PARTICLES
DK3044339T3 (da) 2013-09-10 2019-08-12 MockV Solutions Fremgangsmåder og sæt til kvantificering af fjernelsen af falske (MOCK) viruspartikler fra en renset opløsning
RU2701948C1 (ru) * 2015-11-06 2019-10-02 Асахи Касеи Медикал Ко., Лтд. Способ получения парвовируса, характеризующегося высоким титром инфекционности и высокой чистотой
DE102016005049B4 (de) 2016-04-26 2020-06-18 Sartorius Stedim Biotech Gmbh Verfahren zur Bestimmung des logarithmischen Reduktionswertes LRV eines Größenausschlussfilters
EP3299454A1 (en) * 2016-09-21 2018-03-28 Deutsches Krebsforschungszentrum Optimized method for large scale production of parvovirus h-1 in an essentially serum-free medium
EP3141611A3 (en) 2016-10-21 2017-05-10 Bayer Healthcare LLC Validation of continuous viral clearance
US10961512B2 (en) * 2017-04-17 2021-03-30 Dao-Yao He Compositions and kits for purification of viral particles from host cells
KR102453351B1 (ko) * 2017-07-05 2022-10-07 에스케이바이오사이언스(주) 인플루엔자 백신용 바이러스 씨드 스톡의 제조 방법
EP3446710A1 (en) * 2017-08-25 2019-02-27 Glenmark Pharmaceuticals S.A. Methods of inactivating viral contaminants
CN108841794B (zh) * 2018-06-08 2021-11-30 苏州良辰生物医药科技有限公司 一种病毒原液的制备方法
JP7588466B2 (ja) * 2020-01-22 2024-11-22 旭化成メディカル株式会社 パルボウイルスの生産方法
JP7390466B2 (ja) * 2020-02-28 2023-12-01 旭化成メディカル株式会社 ウイルスクリアランス性能の評価方法
US12403428B2 (en) 2020-06-24 2025-09-02 Asahi Kasei Life Science Corporation Evaluation method for protein-containing solution
CA3195893A1 (en) * 2020-09-21 2022-03-24 Bayer Healthcare Llc Pathogen clearance system and method
US12203914B2 (en) 2020-11-12 2025-01-21 Sartorius Bioanalytical Instruments, Inc. Viral clearance evaluation for biological medical product preparation processes
EP4317460A4 (en) 2021-03-26 2024-10-23 Asahi Kasei Medical Co., Ltd. Viral clearance test method
WO2022252098A1 (zh) * 2021-05-31 2022-12-08 中国科学院深圳先进技术研究院 噬菌体制剂的制备方法、药物组合物以及应用
JP7665143B2 (ja) * 2021-06-24 2025-04-21 株式会社島津製作所 ウイルス試料の濃縮方法

Family Cites Families (101)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3622663A (en) 1968-06-13 1971-11-23 American Home Prod Purification of viruses on treated calcium dihydrogen orthophosphate monohydrate adsorbent
US3919044A (en) 1973-03-28 1975-11-11 Armour Pharma Processes for concentrating and purifying viruses and viral antigens
SU579309A1 (ru) 1973-11-16 1977-11-05 Ленинградский Политехнический Институт Им.М.И.Калинина Способ получени вакцины
FR2475572A1 (fr) 1980-02-11 1981-08-14 Pasteur Institut Procede pour l'obtention de fragments de virus a enveloppe lipidique, en particulier d'antigenes utilisables comme vaccins, produits obtenus et applications
JPS6147186A (ja) 1984-08-09 1986-03-07 Chemo Sero Therapeut Res Inst インフルエンザウイルスの精製方法
JPS6147185A (ja) 1984-08-09 1986-03-07 Chemo Sero Therapeut Res Inst 日本脳炎ウイルスの精製方法
JPS6147187A (ja) 1984-08-10 1986-03-07 Chemo Sero Therapeut Res Inst 狂犬病ウイルスの精製方法
US6265149B1 (en) 1986-01-22 2001-07-24 Institut Pasteur In vitro diagnostic methods and kits for the detection of HIV-2-specific antibodies
US5824482A (en) 1986-06-23 1998-10-20 Institut Pasteur Purification, cloning, and characterization of a novel human immunodeficiency virus LAVMAL
US4814268A (en) 1986-10-06 1989-03-21 Kreider John W Methods for propagating fastidious human viruses and for producing purified suspensions thereof
US4859597A (en) * 1987-07-27 1989-08-22 Igene Biotechnology, Inc. Production of phage and phage-associated lysin
NZ225583A (en) 1987-08-06 1991-07-26 Merck & Co Inc Process for purifying hepatitis a virions (hav)
US5268292A (en) 1988-06-27 1993-12-07 Robertson Betty H Reproducible generation of high yields of hepatitis A virus by cell culture
GB8914968D0 (en) 1989-06-29 1989-08-23 Connaught Lab Production of virus and purification of viral envelope proteins for vaccine use
US5719051A (en) 1989-12-22 1998-02-17 Immuno Aktiengesellschaft Perfusion system and a method for the large scale production of virus or virus antigen
DE4003543A1 (de) 1990-02-06 1991-08-08 Orpegen Med Molekularbioforsch Verfahren zur abreicherung von viren in loesungen und zur bestimmung der abreicherungsrate von viren
US6709811B1 (en) 1990-05-24 2004-03-23 The United States Of America As Represented By The Department Of Health And Human Services Versatile reagent for detecting murine leukemia viruses
DE69009066T3 (de) 1990-06-15 2001-07-19 Innogenetics N.V., Gent SIV cpz-ant Retrovirus und seine Anwendungen.
IT1248075B (it) 1991-06-18 1995-01-05 Sclavo Spa Processo per la purificazione del virus dell'epatite a (hav), virus cosi` purificato e composizioni vaccinali che lo contengono.
FR2686097B1 (fr) 1992-01-14 1994-12-30 Rhone Merieux Preparation d'antigenes et de vaccins de virus de la mystery disease, antigenes et vaccins obtenus pour la prevention de cette maladie.
US5658779A (en) 1992-03-20 1997-08-19 Ligochem, Inc. Method of adsorbing viruses from fluid compositions
US5602023A (en) 1992-03-24 1997-02-11 Csatary; Laszlo K. Pharmaceutical product containing live, stabilized virus for the therapy of viral and malignant diseases and process for preparing the same
ES2126620T5 (es) 1992-10-06 2010-05-21 Siemens Healthcare Diagnostics Products Gmbh Retrovirus del grupo de los vih y su utilizacion.
WO1995025789A1 (en) 1994-03-22 1995-09-28 The Immune Response Corporation Highly efficient production and isolation of viral particles
FR2723740B1 (fr) 1994-08-16 1996-11-08 Pasteur Merieux Serums Vacc Procede de preparation d'antigenes du virus grippal, antigenes obtenus et leurs applications
CA2203809C (en) 1994-10-28 2008-06-03 James M. Wilson Recombinant adenovirus and methods of use thereof
US5756341A (en) 1994-11-10 1998-05-26 Immuno Ag Method for controlling the infectivity of viruses
US5837520A (en) 1995-03-07 1998-11-17 Canji, Inc. Method of purification of viral vectors
US6281010B1 (en) 1995-06-05 2001-08-28 The Trustees Of The University Of Pennsylvania Adenovirus gene therapy vehicle and cell line
FR2737730B1 (fr) 1995-08-10 1997-09-05 Pasteur Merieux Serums Vacc Procede de purification de virus par chromatographie
US5840565A (en) 1995-08-22 1998-11-24 The Regents Of The University Of California Methods for enhancing the production of viral vaccines in PKR-deficient cell culture
JPH11511326A (ja) 1995-08-30 1999-10-05 ジエンザイム コーポレイション アデノウィルスおよびaavの精製
AU1733697A (en) 1996-02-29 1997-09-16 Takara Shuzo Co., Ltd. Method of purifying and removing viruses
BR9708401A (pt) 1996-03-29 2000-01-04 Univ Otago Vetores de vìrus de para-varìola
IL127692A0 (en) 1996-07-01 1999-10-28 Rhone Poulenc Rorer Sa Method for producing recombinant adenovirus
AU732703B2 (en) 1996-11-20 2001-04-26 Crucell Holland B.V. An improved method for the production and purification of adenoviral vectors
US7732129B1 (en) 1998-12-01 2010-06-08 Crucell Holland B.V. Method for the production and purification of adenoviral vectors
FR2756297B1 (fr) 1996-11-22 1999-01-08 Centre Nat Rech Scient Procede de production de virus recombinants
US6261823B1 (en) 1996-12-13 2001-07-17 Schering Corporation Methods for purifying viruses
US6544769B1 (en) 1996-12-13 2003-04-08 Schering Corporation Compostions comprising viruses and methods for concentrating virus preparations
ZA982950B (en) 1997-04-08 1998-10-19 Merck & Co Inc Stabilized human papillomavirus formulations
US6989264B2 (en) 1997-09-05 2006-01-24 Targeted Genetics Corporation Methods for generating high titer helper-free preparations of released recombinant AAV vectors
US6410300B1 (en) 1998-01-12 2002-06-25 The University Of North Carolina At Chapel Hill Methods and formulations for mediating adeno-associated virus (AAV) attachment and infection and methods for purifying AAV
US6037456A (en) 1998-03-10 2000-03-14 Biosource Technologies, Inc. Process for isolating and purifying viruses, soluble proteins and peptides from plant sources
US5994134A (en) 1998-05-04 1999-11-30 Canji, Inc. Viral production process
US6146874A (en) 1998-05-27 2000-11-14 University Of Florida Method of preparing recombinant adeno-associated virus compositions
PL347472A1 (en) 1998-08-14 2002-04-08 Merck & Co Inc Process for purifying human papillomavirus virus-like particles
WO2000011149A1 (en) 1998-08-24 2000-03-02 Uab Research Foundation Methods of producing high titer recombinant adeno-associated virus
AU780231B2 (en) 1998-11-10 2005-03-10 University Of North Carolina At Chapel Hill, The Virus vectors and methods of making and administering the same
CN1087032C (zh) * 1998-12-14 2002-07-03 彭朝晖 一种生产重组腺病毒的方法
FR2787465A1 (fr) 1998-12-21 2000-06-23 Transgene Sa Procede d'inactivation des virus enveloppes dans une preparation virale de virus non enveloppes
CA2356373A1 (fr) * 1998-12-31 2000-07-13 Aventis Pharma S.A. Methode de separation de particules virales
US6387368B1 (en) 1999-02-08 2002-05-14 The Trustees Of The University Of Pennsylvania Hybrid adenovirus-AAV virus and methods of use thereof
US6793926B1 (en) 1999-05-27 2004-09-21 Genovo, Inc. Methods for production of a recombinant adeno-associated virus
US6316185B1 (en) 1999-09-29 2001-11-13 Mountain View Pharmaceuticals, Inc. Quantitation of viruses by light scattering
AU1811301A (en) 1999-12-02 2001-06-12 General Hospital Corporation, The Methods for removal, purification, and concentration of viruses, and methods of therapy based thereupon
CA2395820A1 (en) 1999-12-29 2001-07-05 Genzyme Corporation Method using filtration aids for the separation of virus vectors from nucleic acids and other cellular contaminants
TWI289158B (en) 2000-08-10 2007-11-01 Oncolytics Biotech Inc Method of producing infectious reovirus
WO2002018550A1 (en) 2000-08-25 2002-03-07 The General Hospital Corporation Selective precipitation of viruses
DE10144903A1 (de) 2001-09-12 2003-03-27 Chiron Behring Gmbh & Co Vermehrung von Viren in Zellkultur
US6825027B2 (en) 2001-12-10 2004-11-30 Baxter Healthcare S.A. Method of production of purified hepatitis a virus particles and vaccine preparation
US7195905B2 (en) 2001-12-10 2007-03-27 Baxter Healthcare S.A. Enveloped virus vaccine and method for production
US6951752B2 (en) 2001-12-10 2005-10-04 Bexter Healthcare S.A. Method for large scale production of virus antigen
WO2003049763A1 (en) 2001-12-12 2003-06-19 Fh Faulding & Co Limited Composition for the preservation of viruses
US20050118701A1 (en) 2002-03-29 2005-06-02 Weichang Zhou Large scale methods of producing adenovirus and adenovirus seed stocks
WO2003085138A1 (en) 2002-03-29 2003-10-16 Merck & Co., Inc. Methods of virus production
ATE427990T1 (de) 2002-04-30 2009-04-15 Oncolytics Biotech Inc Verbesserte reinigungsmethode fur viren
WO2003097797A2 (en) 2002-05-14 2003-11-27 Merck & Co., Inc. Methods of adenovirus purification
US7419817B2 (en) 2002-05-17 2008-09-02 The United States Of America As Represented By The Secretary Department Of Health And Human Services, Nih. Scalable purification of AAV2, AAV4 or AAV5 using ion-exchange chromatography
US20040033585A1 (en) 2002-06-07 2004-02-19 Mccormick Alison A. Flexible vaccine assembly and vaccine delivery platform
AU2003279240A1 (en) 2002-06-21 2004-01-06 Genetix Pharmaceuticals, Inc. Methods for purifying viral particles for gene therapy
CA2491805A1 (en) * 2002-07-10 2004-01-22 Transgene S.A. Modified adenoviral fiber with ablated binding to cellular receptors
UA85543C2 (ru) 2002-09-05 2009-02-10 Бавариан Нордик А/С Способ культивирования первичных клеток и амплификации вирусов в условиях безсывороточной среды, композиция и ее применение, и способ лечения или вакцинации животных
US20050009168A1 (en) 2003-05-12 2005-01-13 Robbins Joan Marie Methods and apparatus for Adeno associated virus purification
AU2004249199B2 (en) 2003-06-18 2008-07-24 Onyx Pharmaceuticals, Inc. Method for purifying virus
AU2003272868A1 (en) 2003-10-15 2005-04-27 Agtc Gene Technology Company Ltd. Method for large-scale production, isolation, purification and the uses of multi-type recombinant adeno-associated virus vectors
US20050153419A1 (en) 2003-12-23 2005-07-14 Schering Corporation Methods for producing cell lines stable in serum-free medium suspension culture
ES2329607T3 (es) 2004-02-23 2009-11-27 Crucell Holland B.V. Metodos de purificacion de virus.
NZ551640A (en) 2004-05-20 2010-05-28 Id Biomedical Corp Process for the production of an influenza vaccine
EP4170024A1 (en) 2004-06-01 2023-04-26 Genzyme Corporation Compositions and methods to prevent aav vector aggregation
DE102004049290A1 (de) 2004-10-09 2006-04-20 Bayer Healthcare Ag Verfahren zur Herstellung von Virusmaterial
US7901921B2 (en) * 2004-10-22 2011-03-08 Oncolytics Biotech Inc. Viral purification methods
CA2586107A1 (en) 2004-11-03 2006-05-18 Introgen Therapeutics Inc. A novel method for the production and purification of adenoviral vectors
US20060281075A1 (en) 2004-12-22 2006-12-14 Large Scale Biology Corporation Purification of viruses, proteins and nucleic acids
US7344839B2 (en) 2004-12-23 2008-03-18 Aurx, Inc. Virus preparations and methods
DK1869171T4 (en) 2005-04-11 2016-01-18 Crucell Holland Bv Virus cleaning using ultrafiltration
DE102005047301B4 (de) 2005-09-30 2009-04-16 Sartorius Stedim Biotech Gmbh Verfahren zum Nachweis der Virenabreicherung für die Validierung von Filtern und Filtrationsprozessen
WO2007059473A2 (en) 2005-11-12 2007-05-24 Introgen Therapeutics, Inc. Methods for the production and purification of adenoviral vectors
US7754421B2 (en) 2005-11-18 2010-07-13 National Research Council Of Canada Detection of intact recombinant viruses
KR101157176B1 (ko) 2005-12-20 2012-06-20 삼성전자주식회사 세포 또는 바이러스의 농축 또는 정제용 미세유동장치 및방법
EP2018421B1 (en) * 2006-04-28 2012-12-19 The Trustees of the University of Pennsylvania Scalable production method for aav
US7851196B2 (en) 2006-05-01 2010-12-14 The Regents Of The University Of California Methods for purifying adeno-associated virus particles
CN101516395B (zh) * 2006-09-01 2014-03-26 伯哈拉特生物技术国际有限公司 抗屈曲病毒感染的疫苗
CA2671869A1 (en) 2006-12-15 2008-06-26 Schering-Plough Ltd. Method for replicating influenza virus in culture
WO2008109721A1 (en) * 2007-03-06 2008-09-12 Introgen Therapeutics, Inc. Chromatographic methods for assessing adenovirus purity
KR20170001720A (ko) * 2007-03-14 2017-01-04 다케다 백신즈 인코포레이티드 바이러스 유사 입자 정제
CA2685497A1 (en) 2007-03-27 2008-10-02 University Of Maryland, College Park Imprinted polymeric materials for binding various targets such as viruses
KR101744430B1 (ko) 2007-05-04 2017-06-20 백스터 인터내셔널 인코포레이티드 바이러스 정제를 위한 연속 초원심분리용 당 용액의 제조
JP2011511640A (ja) * 2008-02-12 2011-04-14 サノフィ パストゥール リミテッド ポックス・ウイルスの精製方法
BR112012026095A2 (pt) 2010-04-14 2015-09-15 Emd Millipore Corp métodos de produção de estoques de vírus de alta pureza e altos títulos e métodos de uso dos mesmos.
US9651469B1 (en) * 2016-01-27 2017-05-16 General Electric Company Electrostatic particle sensor

Also Published As

Publication number Publication date
JP6522054B2 (ja) 2019-05-29
US20120088228A1 (en) 2012-04-12
JP2013523175A (ja) 2013-06-17
JP2015126757A (ja) 2015-07-09
CN102985536B (zh) 2017-12-05
US9644187B2 (en) 2017-05-09
WO2011130119A3 (en) 2012-03-29
KR20130020954A (ko) 2013-03-04
WO2011130119A8 (en) 2011-12-29
EP2377927A1 (en) 2011-10-19
CN102985536A (zh) 2013-03-20
BR112012026095A2 (pt) 2015-09-15
JP6284500B2 (ja) 2018-02-28
ES2736166T3 (es) 2019-12-26
KR101549296B1 (ko) 2015-09-01
EP2377927B1 (en) 2019-05-22
WO2011130119A2 (en) 2011-10-20
JP2017195897A (ja) 2017-11-02

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