RU2431671C2 - Химерные, гибридные и тандемные полипептиды менингококкового белка nmb1870 - Google Patents

Химерные, гибридные и тандемные полипептиды менингококкового белка nmb1870 Download PDF

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RU2431671C2
RU2431671C2 RU2008125838/10A RU2008125838A RU2431671C2 RU 2431671 C2 RU2431671 C2 RU 2431671C2 RU 2008125838/10 A RU2008125838/10 A RU 2008125838/10A RU 2008125838 A RU2008125838 A RU 2008125838A RU 2431671 C2 RU2431671 C2 RU 2431671C2
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polypeptide
polypeptide according
chimeric
hybrid
seq
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Вега МАЗИНЬЯНИ (IT)
Вега МАЗИНЬЯНИ
Мария СКАРСЕЛЛИ (IT)
Мария СКАРСЕЛЛИ
Рино РАППУОЛИ (IT)
Рино РАППУОЛИ
Марияграция ПИЦЦА (IT)
Марияграция ПИЦЦА
Марция ДЖУЛИАНИ (IT)
Марция ДЖУЛИАНИ
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Новартис Вэксинес Энд Дайэгностикс Срл
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/22—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Neisseriaceae (F)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/164—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/095—Neisseria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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Abstract

Изобретение относится к биотехнологии. Предложен полипептид, обладающий способностью индуцировать антитела, являющиеся бактерицидными в отношении штаммов в каждом из трех семейств NMB 1870 I-III. Предложена иммуногенная композиция, содержащая данный полипептид. Предложена нуклеиновая кислота, кодирующая данный полипептид. Полипептиды настоящего изобретения могут вызывать гуморальный иммунный ответ, являющийся бактерицидным в отношении менингококков. 3 н. и 5 з.п. ф-лы.

Description

Текст описания приведен в факсимильном виде.
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Claims (8)

1. Полипептид, имеющий аминокислотную последовательность, по меньшей мере, на 85% идентичную последовательности SEQ ID NO:57, и в которой один или более из следующих остатков, по сравнению с SEQ ID NO:57, замещен на другую аминокислоту: А67, K70, K85, Т147, N149,I39, K48, Е51, R54, Т56, А78, А79, D97, Р105, S114, S116, L1 18, N120, Q121, A 122 и где полипептид обладает способностью индуцировать антитела, являющиеся бактерицидными в отношении штаммов в каждом из трех семейств NMB 1870 I-III.
2. Полипептид по п.1, в котором, по меньшей мере, один из следующих остатков замещен: K48, Е51, R54, А79, K85, Р105, L118, N120, Q121, А122, Т147, N149.
3. Полипептид по п.1, в котором, по меньшей мере, один из следующих остатков замещен: I39, Т56, K70, А78, А79, K85, D97, S114, S116, L118.
4. Полипептид по п.2 или 3, содержащий замену, выбранную из: K70R, K85R, T147I, N149E, I39L, K48Q, R54K, Т56Е, А78Т, А79К, D97E, Р105А, Sl14L, Sl16D, L118R, N120G, Q121S, А122Е.
5. Полипептид по пп. 1-3, где аминокислотная последовательность, по меньшей мере, на 90% идентична последовательности SEQ ID NO:57.
6. Иммуногенная композиция, содержащая эффективное количество полипептида по любому из предшествующих пунктов.
7. Композиция по п.6, содержащая адъювант.
8. Нуклеиновая кислота, кодирующая полипептид по любому из пп.1-5.
RU2008125838/10A 2005-11-25 2006-11-27 Химерные, гибридные и тандемные полипептиды менингококкового белка nmb1870 RU2431671C2 (ru)

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