RU2012126077A - Событие 416 aad-12, родственные линии трансгенной сои и их событиеспецифичная идентификация - Google Patents
Событие 416 aad-12, родственные линии трансгенной сои и их событиеспецифичная идентификация Download PDFInfo
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Abstract
1. Растение трансгенной сои, которое имеет геном, включающий последовательность SEQ ID NO:1.2. Семя сои, которое имеет геном, включающий событие pDAB4468-0416 сои AAD-12, присутствующее в семени, депонированном в Американскую коллекцию типовых культур (АТСС) под номером доступа РТА-10442.3. Семя сои, которое имеет геном, включающий последовательность SEQ ID NO:1.4. Растение сои, полученное в результате выращивания семени по п.2, которое включает последовательность SEQ ID NO:1.5. Потомство растения сои по п.4, включающее событие pDAB4468-0416 сои AAD-12.6. Потомство растения сои по п.1, устойчивое к гербициду, которое включает последовательность SEQ ID NO:1.7. Растение трансгенной сои, включающее вставку трансгена на сайте-мишени, локализованном в хромосоме 4 между маркерами SNP последовательностей SEQ ID NO:29 и SEQ ID NO:30, где указанный сайт-мишень включает гетерологичную нуклеиновую кислоту.8. Способ получения растения трансгенной сои, который включает введение гетерологичной нуклеиновой кислоты в хромосому 4 в положение между маркерами SNP последовательностей SEQ ID NO:29 и SEQ ID NO:30, где указанный сайт-мишень включает гетерологичную нуклеиновую кислоту.9. Часть растения по п.4, выбираемая из группы, состоящей из пыльцы, семяпочки, цветков, семенных коробочек, линта, побегов, корней и листьев, которая включает последовательность SEQ ID NO:1.10. Растение трансгенной сои, включающее вставку трансгена в геномной последовательности или фланкированную геномной последовательностью, выбираемой из группы, состоящей из остатков 1-2730 SEQ ID NO:1 и остатков 9122-10212 SEQ ID NO:1.11. Выделенная полинуклеотидная молекула, которая включает по меньшей мере 15 нуклеотидов и остается гибридизированной в строгих
Claims (37)
1. Растение трансгенной сои, которое имеет геном, включающий последовательность SEQ ID NO:1.
2. Семя сои, которое имеет геном, включающий событие pDAB4468-0416 сои AAD-12, присутствующее в семени, депонированном в Американскую коллекцию типовых культур (АТСС) под номером доступа РТА-10442.
3. Семя сои, которое имеет геном, включающий последовательность SEQ ID NO:1.
4. Растение сои, полученное в результате выращивания семени по п.2, которое включает последовательность SEQ ID NO:1.
5. Потомство растения сои по п.4, включающее событие pDAB4468-0416 сои AAD-12.
6. Потомство растения сои по п.1, устойчивое к гербициду, которое включает последовательность SEQ ID NO:1.
7. Растение трансгенной сои, включающее вставку трансгена на сайте-мишени, локализованном в хромосоме 4 между маркерами SNP последовательностей SEQ ID NO:29 и SEQ ID NO:30, где указанный сайт-мишень включает гетерологичную нуклеиновую кислоту.
8. Способ получения растения трансгенной сои, который включает введение гетерологичной нуклеиновой кислоты в хромосому 4 в положение между маркерами SNP последовательностей SEQ ID NO:29 и SEQ ID NO:30, где указанный сайт-мишень включает гетерологичную нуклеиновую кислоту.
9. Часть растения по п.4, выбираемая из группы, состоящей из пыльцы, семяпочки, цветков, семенных коробочек, линта, побегов, корней и листьев, которая включает последовательность SEQ ID NO:1.
10. Растение трансгенной сои, включающее вставку трансгена в геномной последовательности или фланкированную геномной последовательностью, выбираемой из группы, состоящей из остатков 1-2730 SEQ ID NO:1 и остатков 9122-10212 SEQ ID NO:1.
11. Выделенная полинуклеотидная молекула, которая включает по меньшей мере 15 нуклеотидов и остается гибридизированной в строгих условиях промывки с последовательностью нуклеиновой кислоты, выбираемой из группы, состоящей из остатков 1-2730 SEQ ID NO:1 и остатков 9122-10212 SEQ ID NO:1.
12. Выделенный полинуклеотид, который включает нуклеотидную последовательность, выбираемую из группы, состоящей из SEQ ID NO:2-28.
13. Выделенный полинуклеотид, который гибридизирует в строгих условиях промывки с нуклеотидной последовательностью, выбираемой из группы, состоящей из остатков 2720-2740 SEQ ID NO:1, остатков 9112-9132 SEQ ID NO:1, и ее комплементами.
14. Полинуклеотид по п.13, который является ампликоном, полученным при помощи полимеразной цепной реакции.
15. Полинуклеотид, включающий SEQ ID NO:1.
16. Способ получения полинуклеотида по п.15.
17. Способ введения трансгена в сегмент ДНК генома сои, в котором 5'-конец включает остатки 1-2730 SEQ ID NO:1 и 3'-конец включает остатки 9122-10212 SEQ ID NO:1.
18. Способ селекции растения сои, который включает скрещивание первого растения сои, включающего SEQ ID NO:1, со вторым растением сои с целью получения третьего растения сои, имеющего геном, и анализ указанного третьего растения сои на наличие SEQ ID NO:1 в указанном геноме.
19. Способ интрогрессии признака устойчивости к гербициду в растение сои, который включает скрещивание первого растения сои, включающего SEQ ID NO:1, со вторым растением сои с целью получения третьего растения сои, имеющего геном, и анализ указанного третьего растения сои на наличие SEQ ID NO:1 в указанном геноме.
20. Способ уничтожения сорняков, который включает применение гербицида на основе арилоксиалканоата в поле, на котором произрастает растение по п.1.
21. Способ по п.20, в котором указанный гербицид выбирают из группы, состоящей из 2,4-D, 2,4-DB, MCPA и МСРВ.
22. Способ по п.20, который включает применение второго гербицида в указанном поле.
23. Способ по п.22, в котором указанный второй гербицид выбирают из группы, состоящей из глифосата и дикамба.
24. Способ уничтожения сорняков, который включает применение гербицида на основе арилоксиалканоата в поле и посев семени по п.3 на указанном поле в течение 14 дней после внесения гербицида.
25. Способ детекции события сои в образце, содержащем ДНК сои, который включает контактирование указанного образца по меньшей мере с одним полинуклеотидом, позволяющим диагностировать событие pDAB4468-0416 сои AAD-12, присутствующее в семени, депонированном в Американскую коллекцию типовых культур (АТСС) под номером доступа РТА-10442.
26. Способ по п.25, который включает контактирование указанного образца:
а) с первой затравкой, которая связывается с фланкирующей последовательностью, выбираемой из группы, состоящей из остатков 1-2730 SEQ ID NO:1, остатков 9122-10212 SEQ ID NO:1, и ее комплементами; и
b) со второй затравкой, которая связывается с вставочной последовательностью, включающей остатки 2731-9121 SEQ ID NO:1, или ее комплементом;
выполнение полимеразной цепной реакции указанного образца и анализ ампликона, полученного между указанными затравками.
27. Способ по п.26, в котором указанные затравки выбирают из группы, состоящей из SEQ ID NO:2-7.
28. Способ по п.25, в котором указанный полинуклеотид, включающий по меньшей мере 30 нуклеотидов, гибридизирует в строгих условиях с последовательностью, выбираемой из группы, состоящей из остатков 2720-2740 SEQ ID NO:1, остатков 9112-9132 SEQ ID NO:1, и ее комплементами; при этом указанный способ далее включает гибридизацию в строгих условиях указанного образца и указанного полинуклеотида; и анализ указанного образца на наличие гибридизации указанного полинуклеотида с указанной ДНК.
29. Набор для детекции ДНК, который включает первую затравку и вторую затравку по п.27.
30. Набор для детекции ДНК, который предназначен для осуществления способа по п.28.
31. Набор для детекции ДНК, включающий полинуклеотид, в котором указанный полинуклеотид включает по меньшей мере 30 нуклеотидов и гибридизирует в строгих условиях с последовательностью, выбираемой из группы, состоящей из остатков 2720-2740 SEQ ID NO:1, остатков 9112-9132 SEQ ID NO:1, и ее комплементами.
32. Способ по п.20, причем указанный способ используют для обработки сорняков, устойчивых к гербицидам, или предотвращения появления указанных сорняков.
33. Способ по п.20, в котором указанный гербицид вносят до посева указанного растения.
34. Способ уничтожения сорняков, устойчивых к глифосату, на участке произрастания по меньшей мере одного растения по п.1, имеющего признак устойчивости к глифосату, который включает применение гербицида на основе арилоксиалканоата по меньшей мере на части указанного участка.
35. Способ по п.34, в котором указанный гербицид является феноксиауксином.
36. Способ по п.34, в котором указанный гербицид вносят в виде смеси с глифосатом.
37. Способ по п.20, в котором по меньшей мере один из указанных сорняков является растением-самосевом, устойчивым к глифосату и относящимся к виду, отличному от вида указанного растения.
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PCT/US2010/058001 WO2011066384A1 (en) | 2009-11-24 | 2010-11-24 | Aad-12 event 416, related transgenic soybean lines, and event-specific identification thereof |
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ES2637948T3 (es) * | 2005-10-28 | 2017-10-18 | Dow Agrosciences Llc | Nuevos genes de resistencia a herbicidas |
US8685677B2 (en) * | 2009-11-24 | 2014-04-01 | Dow Agrosciences, Llc. | Detection of AAD-12 soybean event 416 |
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