RU2012126077A - Событие 416 aad-12, родственные линии трансгенной сои и их событиеспецифичная идентификация - Google Patents
Событие 416 aad-12, родственные линии трансгенной сои и их событиеспецифичная идентификация Download PDFInfo
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- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
- A01H1/122—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- A01H1/123—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/10—Seeds
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- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/54—Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
- A01H6/542—Glycine max [soybean]
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- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Abstract
1. Растение трансгенной сои, которое имеет геном, включающий последовательность SEQ ID NO:1.2. Семя сои, которое имеет геном, включающий событие pDAB4468-0416 сои AAD-12, присутствующее в семени, депонированном в Американскую коллекцию типовых культур (АТСС) под номером доступа РТА-10442.3. Семя сои, которое имеет геном, включающий последовательность SEQ ID NO:1.4. Растение сои, полученное в результате выращивания семени по п.2, которое включает последовательность SEQ ID NO:1.5. Потомство растения сои по п.4, включающее событие pDAB4468-0416 сои AAD-12.6. Потомство растения сои по п.1, устойчивое к гербициду, которое включает последовательность SEQ ID NO:1.7. Растение трансгенной сои, включающее вставку трансгена на сайте-мишени, локализованном в хромосоме 4 между маркерами SNP последовательностей SEQ ID NO:29 и SEQ ID NO:30, где указанный сайт-мишень включает гетерологичную нуклеиновую кислоту.8. Способ получения растения трансгенной сои, который включает введение гетерологичной нуклеиновой кислоты в хромосому 4 в положение между маркерами SNP последовательностей SEQ ID NO:29 и SEQ ID NO:30, где указанный сайт-мишень включает гетерологичную нуклеиновую кислоту.9. Часть растения по п.4, выбираемая из группы, состоящей из пыльцы, семяпочки, цветков, семенных коробочек, линта, побегов, корней и листьев, которая включает последовательность SEQ ID NO:1.10. Растение трансгенной сои, включающее вставку трансгена в геномной последовательности или фланкированную геномной последовательностью, выбираемой из группы, состоящей из остатков 1-2730 SEQ ID NO:1 и остатков 9122-10212 SEQ ID NO:1.11. Выделенная полинуклеотидная молекула, которая включает по меньшей мере 15 нуклеотидов и остается гибридизированной в строгих
Claims (37)
1. Растение трансгенной сои, которое имеет геном, включающий последовательность SEQ ID NO:1.
2. Семя сои, которое имеет геном, включающий событие pDAB4468-0416 сои AAD-12, присутствующее в семени, депонированном в Американскую коллекцию типовых культур (АТСС) под номером доступа РТА-10442.
3. Семя сои, которое имеет геном, включающий последовательность SEQ ID NO:1.
4. Растение сои, полученное в результате выращивания семени по п.2, которое включает последовательность SEQ ID NO:1.
5. Потомство растения сои по п.4, включающее событие pDAB4468-0416 сои AAD-12.
6. Потомство растения сои по п.1, устойчивое к гербициду, которое включает последовательность SEQ ID NO:1.
7. Растение трансгенной сои, включающее вставку трансгена на сайте-мишени, локализованном в хромосоме 4 между маркерами SNP последовательностей SEQ ID NO:29 и SEQ ID NO:30, где указанный сайт-мишень включает гетерологичную нуклеиновую кислоту.
8. Способ получения растения трансгенной сои, который включает введение гетерологичной нуклеиновой кислоты в хромосому 4 в положение между маркерами SNP последовательностей SEQ ID NO:29 и SEQ ID NO:30, где указанный сайт-мишень включает гетерологичную нуклеиновую кислоту.
9. Часть растения по п.4, выбираемая из группы, состоящей из пыльцы, семяпочки, цветков, семенных коробочек, линта, побегов, корней и листьев, которая включает последовательность SEQ ID NO:1.
10. Растение трансгенной сои, включающее вставку трансгена в геномной последовательности или фланкированную геномной последовательностью, выбираемой из группы, состоящей из остатков 1-2730 SEQ ID NO:1 и остатков 9122-10212 SEQ ID NO:1.
11. Выделенная полинуклеотидная молекула, которая включает по меньшей мере 15 нуклеотидов и остается гибридизированной в строгих условиях промывки с последовательностью нуклеиновой кислоты, выбираемой из группы, состоящей из остатков 1-2730 SEQ ID NO:1 и остатков 9122-10212 SEQ ID NO:1.
12. Выделенный полинуклеотид, который включает нуклеотидную последовательность, выбираемую из группы, состоящей из SEQ ID NO:2-28.
13. Выделенный полинуклеотид, который гибридизирует в строгих условиях промывки с нуклеотидной последовательностью, выбираемой из группы, состоящей из остатков 2720-2740 SEQ ID NO:1, остатков 9112-9132 SEQ ID NO:1, и ее комплементами.
14. Полинуклеотид по п.13, который является ампликоном, полученным при помощи полимеразной цепной реакции.
15. Полинуклеотид, включающий SEQ ID NO:1.
16. Способ получения полинуклеотида по п.15.
17. Способ введения трансгена в сегмент ДНК генома сои, в котором 5'-конец включает остатки 1-2730 SEQ ID NO:1 и 3'-конец включает остатки 9122-10212 SEQ ID NO:1.
18. Способ селекции растения сои, который включает скрещивание первого растения сои, включающего SEQ ID NO:1, со вторым растением сои с целью получения третьего растения сои, имеющего геном, и анализ указанного третьего растения сои на наличие SEQ ID NO:1 в указанном геноме.
19. Способ интрогрессии признака устойчивости к гербициду в растение сои, который включает скрещивание первого растения сои, включающего SEQ ID NO:1, со вторым растением сои с целью получения третьего растения сои, имеющего геном, и анализ указанного третьего растения сои на наличие SEQ ID NO:1 в указанном геноме.
20. Способ уничтожения сорняков, который включает применение гербицида на основе арилоксиалканоата в поле, на котором произрастает растение по п.1.
21. Способ по п.20, в котором указанный гербицид выбирают из группы, состоящей из 2,4-D, 2,4-DB, MCPA и МСРВ.
22. Способ по п.20, который включает применение второго гербицида в указанном поле.
23. Способ по п.22, в котором указанный второй гербицид выбирают из группы, состоящей из глифосата и дикамба.
24. Способ уничтожения сорняков, который включает применение гербицида на основе арилоксиалканоата в поле и посев семени по п.3 на указанном поле в течение 14 дней после внесения гербицида.
25. Способ детекции события сои в образце, содержащем ДНК сои, который включает контактирование указанного образца по меньшей мере с одним полинуклеотидом, позволяющим диагностировать событие pDAB4468-0416 сои AAD-12, присутствующее в семени, депонированном в Американскую коллекцию типовых культур (АТСС) под номером доступа РТА-10442.
26. Способ по п.25, который включает контактирование указанного образца:
а) с первой затравкой, которая связывается с фланкирующей последовательностью, выбираемой из группы, состоящей из остатков 1-2730 SEQ ID NO:1, остатков 9122-10212 SEQ ID NO:1, и ее комплементами; и
b) со второй затравкой, которая связывается с вставочной последовательностью, включающей остатки 2731-9121 SEQ ID NO:1, или ее комплементом;
выполнение полимеразной цепной реакции указанного образца и анализ ампликона, полученного между указанными затравками.
27. Способ по п.26, в котором указанные затравки выбирают из группы, состоящей из SEQ ID NO:2-7.
28. Способ по п.25, в котором указанный полинуклеотид, включающий по меньшей мере 30 нуклеотидов, гибридизирует в строгих условиях с последовательностью, выбираемой из группы, состоящей из остатков 2720-2740 SEQ ID NO:1, остатков 9112-9132 SEQ ID NO:1, и ее комплементами; при этом указанный способ далее включает гибридизацию в строгих условиях указанного образца и указанного полинуклеотида; и анализ указанного образца на наличие гибридизации указанного полинуклеотида с указанной ДНК.
29. Набор для детекции ДНК, который включает первую затравку и вторую затравку по п.27.
30. Набор для детекции ДНК, который предназначен для осуществления способа по п.28.
31. Набор для детекции ДНК, включающий полинуклеотид, в котором указанный полинуклеотид включает по меньшей мере 30 нуклеотидов и гибридизирует в строгих условиях с последовательностью, выбираемой из группы, состоящей из остатков 2720-2740 SEQ ID NO:1, остатков 9112-9132 SEQ ID NO:1, и ее комплементами.
32. Способ по п.20, причем указанный способ используют для обработки сорняков, устойчивых к гербицидам, или предотвращения появления указанных сорняков.
33. Способ по п.20, в котором указанный гербицид вносят до посева указанного растения.
34. Способ уничтожения сорняков, устойчивых к глифосату, на участке произрастания по меньшей мере одного растения по п.1, имеющего признак устойчивости к глифосату, который включает применение гербицида на основе арилоксиалканоата по меньшей мере на части указанного участка.
35. Способ по п.34, в котором указанный гербицид является феноксиауксином.
36. Способ по п.34, в котором указанный гербицид вносят в виде смеси с глифосатом.
37. Способ по п.20, в котором по меньшей мере один из указанных сорняков является растением-самосевом, устойчивым к глифосату и относящимся к виду, отличному от вида указанного растения.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US26395009P | 2009-11-24 | 2009-11-24 | |
| US61/263,950 | 2009-11-24 | ||
| PCT/US2010/058001 WO2011066384A1 (en) | 2009-11-24 | 2010-11-24 | Aad-12 event 416, related transgenic soybean lines, and event-specific identification thereof |
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| Publication Number | Publication Date |
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| RU2012126077A true RU2012126077A (ru) | 2013-12-27 |
| RU2603252C2 RU2603252C2 (ru) | 2016-11-27 |
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| RU2012126077/10A RU2603252C2 (ru) | 2009-11-24 | 2010-11-24 | Событие 416 aad-12, родственные линии трансгенной сои и их событиеспецифичная идентификация |
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| US (1) | US9944944B2 (ru) |
| EP (1) | EP2503871B1 (ru) |
| JP (2) | JP6261863B2 (ru) |
| KR (1) | KR101906605B1 (ru) |
| CN (2) | CN104928256A (ru) |
| AR (6) | AR079149A1 (ru) |
| AU (1) | AU2010324752B2 (ru) |
| BR (1) | BR112012012511A2 (ru) |
| CA (1) | CA2781375C (ru) |
| CL (1) | CL2012001341A1 (ru) |
| CO (1) | CO6640202A2 (ru) |
| HK (1) | HK1176516A1 (ru) |
| IL (1) | IL219933A (ru) |
| IN (1) | IN2012DN04843A (ru) |
| MX (1) | MX341974B (ru) |
| RU (1) | RU2603252C2 (ru) |
| UA (3) | UA112408C2 (ru) |
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| IN2012DN04901A (ru) * | 2009-11-24 | 2015-09-25 | Dow Agrosciences Llc | |
| WO2012072489A1 (de) | 2010-11-29 | 2012-06-07 | Bayer Cropscience Ag | Alpha-beta-ungesättigte imine |
| EP2460407A1 (de) | 2010-12-01 | 2012-06-06 | Bayer CropScience AG | Wirkstoffkombinationen umfassend Pyridylethylbenzamide und weitere Wirkstoffe |
| CN103281900A (zh) | 2010-12-01 | 2013-09-04 | 拜耳知识产权有限责任公司 | 氟吡菌酰胺用于防治作物中的线虫以及提高产量的用途 |
| EP3382028A1 (en) | 2010-12-03 | 2018-10-03 | Dow AgroSciences LLC | Stacked herbicide tolerance event 8264.44.06.1, related transgenic soybean lines, and detection thereof |
| US9540656B2 (en) | 2010-12-03 | 2017-01-10 | Dow Agrosciences Llc | Stacked herbicide tolerance event 8291.45.36.2, related transgenic soybean lines, and detection thereof |
| UY33860A (es) * | 2011-01-07 | 2012-08-31 | Dow Agrosciences Llc | Tolerancia aumentada de plantas habilitadas por dht a herbicidas auxínicos resultante de diferencias de porciones en estructuras moleculares del herbicida |
| CA2823999C (en) | 2011-03-10 | 2020-03-24 | Bayer Intellectual Property Gmbh | Use of lipochito-oligosaccharide compounds for safeguarding seed safety of treated seeds |
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