RS50488B - Vektori ekspresije koji sadrže mcmv ie2 promotor - Google Patents

Vektori ekspresije koji sadrže mcmv ie2 promotor

Info

Publication number
RS50488B
RS50488B YUP-2005/0687A YUP20050687A RS50488B RS 50488 B RS50488 B RS 50488B YU P20050687 A YUP20050687 A YU P20050687A RS 50488 B RS50488 B RS 50488B
Authority
RS
Serbia
Prior art keywords
mcmv
promoter
expression vectors
expression vector
expression
Prior art date
Application number
YUP-2005/0687A
Other languages
English (en)
Inventor
Markus Imhof
Philippe Chatellard
Original Assignee
Laboratoires Serono Sa.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Laboratoires Serono Sa. filed Critical Laboratoires Serono Sa.
Publication of RS20050687A publication Critical patent/RS20050687A/sr
Publication of RS50488B publication Critical patent/RS50488B/sr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/01—DNA viruses
    • C07K14/03—Herpetoviridae, e.g. pseudorabies virus
    • C07K14/04—Varicella-zoster virus
    • C07K14/045—Cytomegalovirus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16111—Cytomegalovirus, e.g. human herpesvirus 5
    • C12N2710/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16111—Cytomegalovirus, e.g. human herpesvirus 5
    • C12N2710/16141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/16143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/20—Vector systems having a special element relevant for transcription transcription of more than one cistron
    • C12N2830/205—Vector systems having a special element relevant for transcription transcription of more than one cistron bidirectional
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Veterinary Medicine (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Vektor ekspresije koji obuhvata promoter mCMV-IE2 koji se sastoji od fragmenta SEQ ID NO.1 za promociju funkcionalne ekspresije, naznačeno time da:(i) vektor ekspresije ne sadrži nijedan kompletan gen mCMV, i(ii) pomenuti promotor mCMV-IE2 je operabilno povezan sa DNK sekvencom koja kodira polipeptid.Prijava sadrži još 8 nezavisnih i 31 zavisnih patentnih zahteva.
YUP-2005/0687A 2003-03-11 2004-03-10 Vektori ekspresije koji sadrže mcmv ie2 promotor RS50488B (sr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP03100617 2003-03-11
PCT/EP2004/050280 WO2004081167A2 (en) 2003-03-11 2004-03-10 Expression vectors comprising the mcmv ie2 promoter

Publications (2)

Publication Number Publication Date
RS20050687A RS20050687A (en) 2007-11-15
RS50488B true RS50488B (sr) 2010-03-02

Family

ID=32981915

Family Applications (1)

Application Number Title Priority Date Filing Date
YUP-2005/0687A RS50488B (sr) 2003-03-11 2004-03-10 Vektori ekspresije koji sadrže mcmv ie2 promotor

Country Status (25)

Country Link
US (3) US7824907B2 (sr)
EP (1) EP1601776B1 (sr)
JP (3) JP5171033B2 (sr)
KR (1) KR101180139B1 (sr)
CN (1) CN100429315C (sr)
AT (1) ATE399872T1 (sr)
AU (1) AU2004219917B2 (sr)
BR (1) BRPI0408246A (sr)
CA (1) CA2516157C (sr)
CY (1) CY1108344T1 (sr)
DE (1) DE602004014738D1 (sr)
DK (1) DK1601776T3 (sr)
EA (1) EA010096B1 (sr)
ES (1) ES2309508T3 (sr)
HR (1) HRP20050705B1 (sr)
IL (1) IL170754A (sr)
MX (1) MXPA05009608A (sr)
NO (1) NO333818B1 (sr)
PL (1) PL1601776T4 (sr)
PT (1) PT1601776E (sr)
RS (1) RS50488B (sr)
SI (1) SI1601776T1 (sr)
UA (1) UA88138C2 (sr)
WO (1) WO2004081167A2 (sr)
ZA (1) ZA200506400B (sr)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0216648D0 (en) 2002-07-18 2002-08-28 Lonza Biologics Plc Method of expressing recombinant protein in CHO cells
DE602004014738D1 (de) * 2003-03-11 2008-08-14 Serono Lab Expressionvektoren, die den promotor des ie2-gens des maus cytomegalovirus enthalten
AU2004238524B2 (en) * 2003-05-13 2011-06-23 Merck Serono Sa Active variants of the IL-18 binding protein and medical uses thereof
WO2005040384A1 (en) * 2003-10-21 2005-05-06 Applied Research Systems Ars Holding N.V. Minimal dna sequence acting as a chromatin insulator and its use in protein expression
DE602004006157T2 (de) 2003-11-05 2008-01-03 Ares Trading S.A. Verfahren zur aufreinigung von il-18-bindendem protein
ES2292127T3 (es) * 2004-03-01 2008-03-01 Ares Trading S.A. Uso de un medio de cultivo celular exento de suero para la produccion de il-18bp en celulas de mamiferos.
CA2569795C (en) * 2004-06-29 2013-08-13 Ares Trading S.A. Process for the purification of il-18 binding protein
AU2005311246B2 (en) * 2004-12-02 2010-12-02 Merck Serono Sa The Lupac bifunctional marker and its use in protein production
US20090181424A1 (en) 2005-04-22 2009-07-16 Fernando Albericio Mammalian Expression Vector Comprising the MCMV Promoter and First Intron of HCMV Major Immediate Early Gene
WO2006124784A2 (en) * 2005-05-16 2006-11-23 Morphotek, Inc. Regulated vectors for selection of cells exhibiting desired phenotypes
CA2609060C (en) * 2005-06-03 2014-07-15 Urs Weber Production of recombinant il-18 binding protein
ATE529433T1 (de) * 2005-06-10 2011-11-15 Ares Trading Sa Verfahren zur reinigung eines il-18-bindenden proteins
EP1760092A1 (en) 2005-08-26 2007-03-07 Applied Research Systems ARS Holding N.V. System for screening cells for high expression of a protein of interest
PL2227549T3 (pl) * 2007-12-21 2015-12-31 Novartis Ag System selekcji do hodowli komórek eukariotycznych oparty na genie związanego z błoną receptora folianowego
JP2009276724A (ja) * 2008-05-19 2009-11-26 Nitto Denko Corp 光導波路装置の製造方法
MX2011009025A (es) 2009-02-27 2011-09-28 Novartis Ag Sistema de vector de expresion que comprende dos marcadores de seleccion.
JP5502406B2 (ja) * 2009-09-14 2014-05-28 ロンザ・バイオロジクス・ピーエルシー Ｃｈｏ細胞において組換えタンパク質を発現する方法
KR101038126B1 (ko) * 2010-11-30 2011-05-31 주식회사 엘지생명과학 새로운 융합 프로모터 및 이를 포함하는 재조합 벡터
US9249785B2 (en) * 2012-01-31 2016-02-02 Brightsource Industries (Isreal) Ltd. Method and system for operating a solar steam system during reduced-insolation events
EP2711426B1 (en) * 2012-09-24 2015-04-08 Lonza Biologics plc Expression vectors comprising chimeric cytomegalovirus promoter and enhancer sequences
ITFI20120221A1 (it) * 2012-10-22 2014-04-23 Nuovo Pignone Srl "exhaust gas collector and gas turbine"
WO2014133468A1 (en) * 2013-02-28 2014-09-04 Agency For Science, Technology And Research Chimeric promoters for high gene expression level and stability in mammalian cells
CN103173480B (zh) * 2013-03-01 2015-06-24 上海中医药大学附属曙光医院 一种利用双荧光素酶报告基因筛选多药耐药相关microRNA的方法
CN103173481A (zh) * 2013-03-01 2013-06-26 上海中医药大学附属普陀医院 一种含有abcb1基因3′utr序列和报告基因的质粒载体及其构建方法和用途
EP2970956A1 (en) 2013-03-11 2016-01-20 Novartis AG Method of screening cell clones
HUE058885T2 (hu) * 2013-07-31 2022-09-28 Novartis Ag Új szelekciós vektorok és eljárások eukarióta gazdasejtek szelektálására
LT3083677T (lt) 2013-12-20 2019-12-10 Novartis Ag Naujos eukariotinės ląstelės ir dominančio polipeptido rekombinantinės raiškos būdai
EP3604331A1 (en) 2013-12-20 2020-02-05 Novartis AG Novel eukaryotic cells and methods for recombinantly expressing a product of interest
JP6265020B2 (ja) * 2014-04-16 2018-01-24 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
MX368664B (es) 2014-04-29 2019-10-10 Novartis Ag Células novedosas de vertebrados y métodos para la expresión recombinante de un polipéptido de interés.
CN106255757B (zh) * 2014-05-19 2020-08-11 豪夫迈·罗氏有限公司 生产多肽的方法
KR20160068558A (ko) 2014-12-05 2016-06-15 삼성바이오에피스 주식회사 마우스 cmv 프로모터를 포함하는 융합 폴리뉴클레오타이드 및 이를 이용한 목적 폴리펩타이드의 생산 방법
AU2016227644B2 (en) 2015-03-05 2022-06-16 Ab2 Bio Sa IL-18 Binding Protein (IL-18BP) and antibodies in inflammatory diseases
WO2016166088A1 (en) 2015-04-14 2016-10-20 Janssen Vaccines & Prevention B.V. Recombinant adenovirus expressing two transgenes with a bidirectional promoter
ES2823173T3 (es) 2016-01-27 2021-05-06 Just Biotherapeutics Inc Promotor híbrido y usos del mismo
JP7046835B2 (ja) 2016-05-12 2022-04-04 ヤンセン ファッシンズ アンド プリベンション ベーフェー 強力でバランスのとれた双方向性プロモーター
US11001858B2 (en) 2016-06-20 2021-05-11 Janssen Vaccines & Prevention B.V. Potent and balanced bidirectional promoter
KR101864581B1 (ko) * 2016-11-04 2018-07-13 국립암센터 양방향 프로모터를 포함하는 렌티바이러스 발현 벡터 및 이의 용도
KR102111244B1 (ko) 2017-02-09 2020-05-15 얀센 백신스 앤드 프리벤션 비.브이. 이종 유전자의 발현을 위한 강력한 짧은 프로모터
EP3692153A1 (en) 2017-10-02 2020-08-12 Astrazeneca AB Cell lines and methods for increased protein production
BR112021010785A2 (pt) * 2018-12-04 2021-11-16 Catalent Pharma Solutions Llc Vetores para fabricação de proteínas
EP3980547A4 (en) * 2019-06-07 2023-06-28 Biocon Biologics Limited Mammalian expression vectors

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2007A (en) * 1841-03-16 Improvement in the mode of harvesting grain
JPS63502719A (ja) * 1985-12-18 1988-10-13 ジェネティックス・インスチチュ−ト・インコ−ポレ−テッド プロモ−タ−系
US4968615A (en) 1985-12-18 1990-11-06 Ciba-Geigy Corporation Deoxyribonucleic acid segment from a virus
US4963481A (en) * 1985-12-18 1990-10-16 Genetics Institute Inc Promoter system
US20020065213A1 (en) * 1998-01-16 2002-05-30 Robert J. Debs Methods and compositions for nonviral gene delivery
BR0108962A (pt) * 2000-03-03 2002-12-24 Valentis Inc Formulações de ácido nucléico para distribuição de genes e métodos de uso
KR100798545B1 (ko) * 2000-05-05 2008-01-28 어플라이드 리서치 시스템스 에이알에스 홀딩 엔.브이. 죽상경화증의 치료 또는 예방을 위한 ｉｌ-18 저해물질의용도
US7812148B2 (en) * 2001-04-05 2010-10-12 Millipore Corporation Vectors comprising CpG islands without position effect varigation and having increased expression
CA2463310A1 (en) * 2001-06-04 2002-12-12 Ml Laboratories Plc Compositions and methods for high-level, large-scale production of recombinant proteins
DE602004014738D1 (de) * 2003-03-11 2008-08-14 Serono Lab Expressionvektoren, die den promotor des ie2-gens des maus cytomegalovirus enthalten
AU2004238524B2 (en) * 2003-05-13 2011-06-23 Merck Serono Sa Active variants of the IL-18 binding protein and medical uses thereof
WO2005040384A1 (en) 2003-10-21 2005-05-06 Applied Research Systems Ars Holding N.V. Minimal dna sequence acting as a chromatin insulator and its use in protein expression
DE602004006157T2 (de) 2003-11-05 2008-01-03 Ares Trading S.A. Verfahren zur aufreinigung von il-18-bindendem protein
ES2292127T3 (es) 2004-03-01 2008-03-01 Ares Trading S.A. Uso de un medio de cultivo celular exento de suero para la produccion de il-18bp en celulas de mamiferos.
CA2569795C (en) 2004-06-29 2013-08-13 Ares Trading S.A. Process for the purification of il-18 binding protein
AU2005311246B2 (en) * 2004-12-02 2010-12-02 Merck Serono Sa The Lupac bifunctional marker and its use in protein production
CA2609060C (en) 2005-06-03 2014-07-15 Urs Weber Production of recombinant il-18 binding protein
ATE529433T1 (de) 2005-06-10 2011-11-15 Ares Trading Sa Verfahren zur reinigung eines il-18-bindenden proteins
KR101958033B1 (ko) * 2011-04-01 2019-03-13 추가이 세이야쿠 가부시키가이샤 재조합 폴리펩티드의 제조 방법

Also Published As

Publication number Publication date
US20110008839A1 (en) 2011-01-13
JP2006520589A (ja) 2006-09-14
US9051582B2 (en) 2015-06-09
ZA200506400B (en) 2006-12-27
AU2004219917A1 (en) 2004-09-23
BRPI0408246A (pt) 2006-03-01
CN100429315C (zh) 2008-10-29
US20150252383A1 (en) 2015-09-10
EA010096B1 (ru) 2008-06-30
NO20054642L (no) 2005-10-10
SI1601776T1 (sl) 2008-10-31
US7824907B2 (en) 2010-11-02
KR101180139B1 (ko) 2012-09-13
KR20050113193A (ko) 2005-12-01
IL170754A (en) 2010-11-30
RS20050687A (en) 2007-11-15
HRP20050705A2 (en) 2005-12-31
ES2309508T3 (es) 2008-12-16
ATE399872T1 (de) 2008-07-15
CN1759184A (zh) 2006-04-12
CA2516157C (en) 2012-09-18
UA88138C2 (en) 2009-09-25
DE602004014738D1 (de) 2008-08-14
CY1108344T1 (el) 2014-02-12
EP1601776A2 (en) 2005-12-07
EA200501444A1 (ru) 2006-02-24
EP1601776B1 (en) 2008-07-02
WO2004081167A3 (en) 2004-11-04
JP5171033B2 (ja) 2013-03-27
MXPA05009608A (es) 2006-03-21
HRP20050705B1 (hr) 2013-08-31
JP5848202B2 (ja) 2016-01-27
DK1601776T3 (da) 2008-10-13
HK1085766A1 (zh) 2006-09-01
NO333818B1 (no) 2013-09-23
PL1601776T3 (pl) 2008-12-31
JP2015180222A (ja) 2015-10-15
JP2012235787A (ja) 2012-12-06
CA2516157A1 (en) 2004-09-23
PT1601776E (pt) 2008-09-17
US20070258962A1 (en) 2007-11-08
PL1601776T4 (pl) 2009-05-29
AU2004219917B2 (en) 2008-01-31
WO2004081167A2 (en) 2004-09-23

Similar Documents

Publication Publication Date Title
RS50488B (sr) Vektori ekspresije koji sadrže mcmv ie2 promotor
BR0009392A (pt) Polipeptìdeo, sequência de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, métodos para produzir uma sequência de ácido nucleico mutante e para produzir um polipeptìdeo, mutante de um polipeptìdeo com atividade de alafa-amilase, e, uso do polipeptìdeo ou variante
DE60228212D1 (de) Neue expressionsvektoren
ATE352626T1 (de) Polypeptide mit galaktoseoxidaseaktivität und dafür kodierende nukleisäuren
DK0618976T3 (da) Syntetisk DNA-sekvens med forbedret insekticid virkning i majs
ATE319818T1 (de) Polypeptide mit aminopeptidaseaktivität und für diese kodierende nukleinsäuren
NZ595498A (en) Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
EA200900212A1 (ru) Элементы нуклеиновых кислот, обладающие регуляторной функцией
ATE414775T1 (de) Polypeptide mit prolyldipeptidylaminopeptidase- aktivität und dafür kodierende nukleinsäuren
ES2543203T3 (es) Grupo de genes NRPS-PKS, su manipulación y su utilidad
BR0110090A (pt) Proteìna, processos para hidrolisar ligações beta-glucosìdicas e beta-xilosìdicas e para produzir um polipeptìdeo, sequência de ácido nucleico, construção de ácido nucleico, cepa microbiana recombinante, e, sonda de oligonucleotìdeo
NZ580378A (en) Recombinant expression vector elements (reves) for enhancing expression of recombinant proteins in host cells
NO980021L (no) Rekombinant protein som har bakteriofag endosialidase-enzymatiske aktivitet
DE60043428D1 (de) POLYPEPTIDE MIT VERZWEIGUNGSENZYM-AKTIVITäT UND FüR DIESE KODIERENDE NUKLEINSäUREN
ES2376225T3 (es) Genes de elongasa y usos de los mismos.
DK0804558T3 (da) Tripeptidylaminopeptidase
ATE358725T1 (de) Polypeptide mit aminopeptidaseaktivität und dafür kodierende nukleinsäuren
ATE362544T1 (de) Polypeptide mit alpha-h alpha amino acid amide racemase aktivitität und nukleinsäuren kodierend dafür
BR0209551A (pt) Promotor de gene especìfico de folha de café
NZ590011A (en) Chloramphenicol acetyl transferase (cat)-defective somatostatin fusion protein and uses thereof
KR960023057A (ko) 니트릴라제 유전자 발현조절인자 및 그 유전자
DE60330579D1 (de) Mikrobielle trypsinvarianten mit chymotrypsinaktivität und diese codierende nukleinsäuren
ATE212060T1 (de) Vom desmingen abstammende amplifizierungssequenzen, vektoren, die diese enthalten und anwendungen derselben zur herstellung von proteinen
NZ592147A (en) Delta-8 desaturase genes, enzymes encoded thereby and uses thereof
WO2001088114A3 (en) Gene expression system