EA200501444A1 - Экспрессирующие векторы, содержащие ie2-промотор mcmv - Google Patents

Экспрессирующие векторы, содержащие ie2-промотор mcmv

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Publication number
EA200501444A1
EA200501444A1 EA200501444A EA200501444A EA200501444A1 EA 200501444 A1 EA200501444 A1 EA 200501444A1 EA 200501444 A EA200501444 A EA 200501444A EA 200501444 A EA200501444 A EA 200501444A EA 200501444 A1 EA200501444 A1 EA 200501444A1
Authority
EA
Eurasian Patent Office
Prior art keywords
mcmv
vectors containing
expressing vectors
gene
mcmv promoter
Prior art date
Application number
EA200501444A
Other languages
English (en)
Other versions
EA010096B1 (ru
Inventor
Филипп Шателлар
Маркус Имхоф
Original Assignee
Апплайд Резеч Системз Арс Холдинг Н.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Апплайд Резеч Системз Арс Холдинг Н.В. filed Critical Апплайд Резеч Системз Арс Холдинг Н.В.
Publication of EA200501444A1 publication Critical patent/EA200501444A1/ru
Publication of EA010096B1 publication Critical patent/EA010096B1/ru

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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/01—DNA viruses
    • C07K14/03—Herpetoviridae, e.g. pseudorabies virus
    • C07K14/04—Varicella-zoster virus
    • C07K14/045—Cytomegalovirus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16111—Cytomegalovirus, e.g. human herpesvirus 5
    • C12N2710/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16111—Cytomegalovirus, e.g. human herpesvirus 5
    • C12N2710/16141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/16143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/20—Vector systems having a special element relevant for transcription transcription of more than one cistron
    • C12N2830/205—Vector systems having a special element relevant for transcription transcription of more than one cistron bidirectional
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Настоящее изобретение относится к экспрессирующему вектору, содержащему IE2-промотор mCMV-гена, либо его фрагмент, стимулирующий функциональную экспрессию, и/или IE2-энхансер mCMV-гена, либо его фрагмент, усиливающий функциональную экспрессию, где указанный экспрессирующий вектор не содержит целого mCMV-гена.Отчет о международном поиске был опубликован 2004.11.04.
EA200501444A 2003-03-11 2004-03-10 Экспрессирующие векторы, содержащие ie2-промотор mcmv EA010096B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP03100617 2003-03-11
PCT/EP2004/050280 WO2004081167A2 (en) 2003-03-11 2004-03-10 Expression vectors comprising the mcmv ie2 promoter

Publications (2)

Publication Number Publication Date
EA200501444A1 true EA200501444A1 (ru) 2006-02-24
EA010096B1 EA010096B1 (ru) 2008-06-30

Family

ID=32981915

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200501444A EA010096B1 (ru) 2003-03-11 2004-03-10 Экспрессирующие векторы, содержащие ie2-промотор mcmv

Country Status (26)

Country Link
US (3) US7824907B2 (ru)
EP (1) EP1601776B1 (ru)
JP (3) JP5171033B2 (ru)
KR (1) KR101180139B1 (ru)
CN (1) CN100429315C (ru)
AT (1) ATE399872T1 (ru)
AU (1) AU2004219917B2 (ru)
BR (1) BRPI0408246A (ru)
CA (1) CA2516157C (ru)
CY (1) CY1108344T1 (ru)
DE (1) DE602004014738D1 (ru)
DK (1) DK1601776T3 (ru)
EA (1) EA010096B1 (ru)
ES (1) ES2309508T3 (ru)
HK (1) HK1085766A1 (ru)
HR (1) HRP20050705B1 (ru)
IL (1) IL170754A (ru)
MX (1) MXPA05009608A (ru)
NO (1) NO333818B1 (ru)
PL (1) PL1601776T4 (ru)
PT (1) PT1601776E (ru)
RS (1) RS50488B (ru)
SI (1) SI1601776T1 (ru)
UA (1) UA88138C2 (ru)
WO (1) WO2004081167A2 (ru)
ZA (1) ZA200506400B (ru)

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CN103173480B (zh) * 2013-03-01 2015-06-24 上海中医药大学附属曙光医院 一种利用双荧光素酶报告基因筛选多药耐药相关microRNA的方法
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KR102288857B1 (ko) 2013-12-20 2021-08-11 노파르티스 아게 신규한 진핵 세포 및 관심 생성물의 재조합 발현 방법
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Also Published As

Publication number Publication date
KR20050113193A (ko) 2005-12-01
HRP20050705A2 (en) 2005-12-31
KR101180139B1 (ko) 2012-09-13
ZA200506400B (en) 2006-12-27
CN100429315C (zh) 2008-10-29
EP1601776B1 (en) 2008-07-02
MXPA05009608A (es) 2006-03-21
RS50488B (sr) 2010-03-02
US20150252383A1 (en) 2015-09-10
NO20054642L (no) 2005-10-10
HRP20050705B1 (hr) 2013-08-31
JP5848202B2 (ja) 2016-01-27
CA2516157C (en) 2012-09-18
CN1759184A (zh) 2006-04-12
DK1601776T3 (da) 2008-10-13
US9051582B2 (en) 2015-06-09
PL1601776T4 (pl) 2009-05-29
JP2015180222A (ja) 2015-10-15
AU2004219917A1 (en) 2004-09-23
CA2516157A1 (en) 2004-09-23
UA88138C2 (en) 2009-09-25
JP2006520589A (ja) 2006-09-14
AU2004219917B2 (en) 2008-01-31
JP2012235787A (ja) 2012-12-06
JP5171033B2 (ja) 2013-03-27
CY1108344T1 (el) 2014-02-12
IL170754A (en) 2010-11-30
ES2309508T3 (es) 2008-12-16
PL1601776T3 (pl) 2008-12-31
DE602004014738D1 (de) 2008-08-14
BRPI0408246A (pt) 2006-03-01
WO2004081167A2 (en) 2004-09-23
US20110008839A1 (en) 2011-01-13
HK1085766A1 (en) 2006-09-01
US20070258962A1 (en) 2007-11-08
WO2004081167A3 (en) 2004-11-04
EA010096B1 (ru) 2008-06-30
EP1601776A2 (en) 2005-12-07
NO333818B1 (no) 2013-09-23
RS20050687A (en) 2007-11-15
ATE399872T1 (de) 2008-07-15
PT1601776E (pt) 2008-09-17
US7824907B2 (en) 2010-11-02
SI1601776T1 (sl) 2008-10-31

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